TY  - JOUR
AU  - Momčilović, Sanja
AU  - Bogdanović, Andrija
AU  - Milošević, Maja
AU  - Mojsilović, Slavko
AU  - Marković, Dragana
AU  - Kočović, Dušica M.
AU  - Vignjević-Petrinović, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1329
AB  - Psychological stress is a significant contributor to various chronic diseases and affects multiple physiological processes including erythropoiesis. This study aimed to examine the tissue-specific contributions of macrophages and extracellular ATP, as a signal of disturbed tissue homeostasis, to erythropoiesis under conditions of repeated psychological stress. Adult male BALB/c mice were subjected to 2 h daily restraint stress for seven consecutive days. Clodronate-liposomes were used to deplete resident macrophages from the bone marrow and spleen two days prior to the first restraint procedure, as well as newly recruited macrophages, every third day for the duration of the experiment. Repeated stress induced a considerable increase in the number of erythroid progenitor cells as well as in the percentage of CD71+/Ter119+ and CD71−/Ter119+ cells in the bone marrow and spleen. Macrophage depletion completely abolished the stimulative effect of repeated stress on immature erythroid cells, and prevented stress-induced increases in ATP levels, P2X7 receptor (P2X7R) expression, and ectonucleotidase CD39 activity and expression in the bone marrow and spleen. The obtained results demonstrate the stimulative effects of repeated stress on erythroid cells, extracellular ATP levels, P2X7R expression, CD39 activity and expression within the bone marrow and spleen, as well as the essential role of macrophages in stress-induced changes.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress
IS  - 14
SP  - 11373
VL  - 24
DO  - 10.3390/ijms241411373
ER  - 

@article{
author = "Momčilović, Sanja and Bogdanović, Andrija and Milošević, Maja and Mojsilović, Slavko and Marković, Dragana and Kočović, Dušica M. and Vignjević-Petrinović, Sanja",
year = "2023",
abstract = "Psychological stress is a significant contributor to various chronic diseases and affects multiple physiological processes including erythropoiesis. This study aimed to examine the tissue-specific contributions of macrophages and extracellular ATP, as a signal of disturbed tissue homeostasis, to erythropoiesis under conditions of repeated psychological stress. Adult male BALB/c mice were subjected to 2 h daily restraint stress for seven consecutive days. Clodronate-liposomes were used to deplete resident macrophages from the bone marrow and spleen two days prior to the first restraint procedure, as well as newly recruited macrophages, every third day for the duration of the experiment. Repeated stress induced a considerable increase in the number of erythroid progenitor cells as well as in the percentage of CD71+/Ter119+ and CD71−/Ter119+ cells in the bone marrow and spleen. Macrophage depletion completely abolished the stimulative effect of repeated stress on immature erythroid cells, and prevented stress-induced increases in ATP levels, P2X7 receptor (P2X7R) expression, and ectonucleotidase CD39 activity and expression in the bone marrow and spleen. The obtained results demonstrate the stimulative effects of repeated stress on erythroid cells, extracellular ATP levels, P2X7R expression, CD39 activity and expression within the bone marrow and spleen, as well as the essential role of macrophages in stress-induced changes.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress",
number = "14",
pages = "11373",
volume = "24",
doi = "10.3390/ijms241411373"
}

Momčilović, S., Bogdanović, A., Milošević, M., Mojsilović, S., Marković, D., Kočović, D. M.,& Vignjević-Petrinović, S.. (2023). Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 24(14), 11373.
https://doi.org/10.3390/ijms241411373

Momčilović S, Bogdanović A, Milošević M, Mojsilović S, Marković D, Kočović DM, Vignjević-Petrinović S. Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress. in International Journal of Molecular Sciences. 2023;24(14):11373.
doi:10.3390/ijms241411373 .

Momčilović, Sanja, Bogdanović, Andrija, Milošević, Maja, Mojsilović, Slavko, Marković, Dragana, Kočović, Dušica M., Vignjević-Petrinović, Sanja, "Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress" in International Journal of Molecular Sciences, 24, no. 14 (2023):11373,
https://doi.org/10.3390/ijms241411373 . .

TY  - CONF
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Božić Nedeljković, Biljana
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Vučetić, Dušan
AU  - Krstić, Milena
AU  - Mladenović, Jovan
AU  - Atanasievska, Sonja
AU  - Protić Đokić, Vesna
AU  - Đorđevski, Nikoleta
AU  - Ristanović, Elizabeta
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1446
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad
T1  - Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi
EP  - 71
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1446
ER  - 

@conference{
author = "Mihaljica, Darko and Marković, Dragana and Božić Nedeljković, Biljana and Veinović, Gorana and Sukara, Ratko and Vučetić, Dušan and Krstić, Milena and Mladenović, Jovan and Atanasievska, Sonja and Protić Đokić, Vesna and Đorđevski, Nikoleta and Ristanović, Elizabeta and Tomanović, Snežana",
year = "2023",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad",
title = "Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi",
pages = "71-70",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1446"
}

Mihaljica, D., Marković, D., Božić Nedeljković, B., Veinović, G., Sukara, R., Vučetić, D., Krstić, M., Mladenović, J., Atanasievska, S., Protić Đokić, V., Đorđevski, N., Ristanović, E.,& Tomanović, S.. (2023). Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 70-71.
https://hdl.handle.net/21.15107/rcub_rimi_1446

Mihaljica D, Marković D, Božić Nedeljković B, Veinović G, Sukara R, Vučetić D, Krstić M, Mladenović J, Atanasievska S, Protić Đokić V, Đorđevski N, Ristanović E, Tomanović S. Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad. 2023;:70-71.
https://hdl.handle.net/21.15107/rcub_rimi_1446 .

Mihaljica, Darko, Marković, Dragana, Božić Nedeljković, Biljana, Veinović, Gorana, Sukara, Ratko, Vučetić, Dušan, Krstić, Milena, Mladenović, Jovan, Atanasievska, Sonja, Protić Đokić, Vesna, Đorđevski, Nikoleta, Ristanović, Elizabeta, Tomanović, Snežana, "Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad (2023):70-71,
https://hdl.handle.net/21.15107/rcub_rimi_1446 .

TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Milošević, Maja
AU  - Marković, Dragana
AU  - Momčilović, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1300
AB  - Stress is an integral part of life. While acute responses to stress are generally regarded as beneficial in dealing with immediate threats, chronic exposure to threatening stimuli exerts deleterious effects and can be either a contributing or an aggravating factor for many chronic diseases including cancer. Chronic psychological stress has been identified as a significant factor contributing to the development and progression of cancer, but the mechanisms that link chronic stress to cancer remain incompletely understood. Psychological stressors initiate multiple physiological responses that result in the activation of the hypothalamic-pituitary-adrenal (HPA) axis, sympathetic nervous system, and the subsequent changes in immune function. Chronic stress exposure disrupts the homeostatic communication between the neuroendocrine and immune systems, shifting immune signaling toward a proinflammatory state. Stress-induced chronic low-grade inflammation and a decline in immune surveillance are both implicated in cancer development and progression. Conversely, tumor-induced inflammatory cytokines, apart from driving a tumor-supportive inflammatory microenvironment, can also exert their biological actions distantly via circulation and therefore adversely affect the stress response. In this minireview, we summarize the current findings on the relationship between stress and cancer, focusing on the role of inflammation in stress-induced neuroendocrine-immune crosstalk. We also discuss the underlying mechanisms and their potential for cancer treatment and prevention.
PB  - Frontiers Media S.A.
T2  - Frontiers in Physiology
T1  - Interplay between stress and cancer—A focus on inflammation
SP  - 1119095
VL  - 14
DO  - 10.3389/fphys.2023.1119095
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Milošević, Maja and Marković, Dragana and Momčilović, Sanja",
year = "2023",
abstract = "Stress is an integral part of life. While acute responses to stress are generally regarded as beneficial in dealing with immediate threats, chronic exposure to threatening stimuli exerts deleterious effects and can be either a contributing or an aggravating factor for many chronic diseases including cancer. Chronic psychological stress has been identified as a significant factor contributing to the development and progression of cancer, but the mechanisms that link chronic stress to cancer remain incompletely understood. Psychological stressors initiate multiple physiological responses that result in the activation of the hypothalamic-pituitary-adrenal (HPA) axis, sympathetic nervous system, and the subsequent changes in immune function. Chronic stress exposure disrupts the homeostatic communication between the neuroendocrine and immune systems, shifting immune signaling toward a proinflammatory state. Stress-induced chronic low-grade inflammation and a decline in immune surveillance are both implicated in cancer development and progression. Conversely, tumor-induced inflammatory cytokines, apart from driving a tumor-supportive inflammatory microenvironment, can also exert their biological actions distantly via circulation and therefore adversely affect the stress response. In this minireview, we summarize the current findings on the relationship between stress and cancer, focusing on the role of inflammation in stress-induced neuroendocrine-immune crosstalk. We also discuss the underlying mechanisms and their potential for cancer treatment and prevention.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Physiology",
title = "Interplay between stress and cancer—A focus on inflammation",
pages = "1119095",
volume = "14",
doi = "10.3389/fphys.2023.1119095"
}

Vignjević-Petrinović, S., Milošević, M., Marković, D.,& Momčilović, S.. (2023). Interplay between stress and cancer—A focus on inflammation. in Frontiers in Physiology
Frontiers Media S.A.., 14, 1119095.
https://doi.org/10.3389/fphys.2023.1119095

Vignjević-Petrinović S, Milošević M, Marković D, Momčilović S. Interplay between stress and cancer—A focus on inflammation. in Frontiers in Physiology. 2023;14:1119095.
doi:10.3389/fphys.2023.1119095 .

Vignjević-Petrinović, Sanja, Milošević, Maja, Marković, Dragana, Momčilović, Sanja, "Interplay between stress and cancer—A focus on inflammation" in Frontiers in Physiology, 14 (2023):1119095,
https://doi.org/10.3389/fphys.2023.1119095 . .

TY  - JOUR
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Kovačić, Marijana
AU  - Vignjević-Petrinović, Sanja
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1288
AB  - Myeloproliferative neoplasms (MPNs) are hematologic malignancies characterized by gene mutations that promote myeloproliferation and resistance to apoptosis via constitutively active signaling pathways, with Janus kinase 2-signal transducers and the activators of transcription (JAK-STAT) axis as a core part. Chronic inflammation has been described as a pivot for the development and advancement of MPNs from early stage cancer to pronounced bone marrow fibrosis, but there are still unresolved questions regarding this issue. The MPN neutrophils are characterized by upregulation of JAK target genes, they are in a state of activation and with deregulated apoptotic machinery. Deregulated neutrophil apoptotic cell death supports inflammation and steers them towards secondary necrosis or neutrophil extracellular trap (NET) formation, a trigger of inflammation both ways. NETs in proinflammatory bone marrow microenvironment induce hematopoietic precursor proliferation, which has an impact on hematopoietic disorders. In MPNs, neutrophils are primed for NET formation, and even though it seems obvious for NETs to intervene in the disease progression by supporting inflammation, no reliable data are available. We discuss in this review the potential pathophysiological relevance of NET formation in MPNs, with the intention of contributing to a better understanding of how neutrophils and neutrophil clonality can orchestrate the evolution of a pathological microenvironment in MPNs.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms
IS  - 5
SP  - 4497
VL  - 24
DO  - 10.3390/ijms24054497
ER  - 

@article{
author = "Marković, Dragana and Maslovarić, Irina and Kovačić, Marijana and Vignjević-Petrinović, Sanja and Ilić, Vesna",
year = "2023",
abstract = "Myeloproliferative neoplasms (MPNs) are hematologic malignancies characterized by gene mutations that promote myeloproliferation and resistance to apoptosis via constitutively active signaling pathways, with Janus kinase 2-signal transducers and the activators of transcription (JAK-STAT) axis as a core part. Chronic inflammation has been described as a pivot for the development and advancement of MPNs from early stage cancer to pronounced bone marrow fibrosis, but there are still unresolved questions regarding this issue. The MPN neutrophils are characterized by upregulation of JAK target genes, they are in a state of activation and with deregulated apoptotic machinery. Deregulated neutrophil apoptotic cell death supports inflammation and steers them towards secondary necrosis or neutrophil extracellular trap (NET) formation, a trigger of inflammation both ways. NETs in proinflammatory bone marrow microenvironment induce hematopoietic precursor proliferation, which has an impact on hematopoietic disorders. In MPNs, neutrophils are primed for NET formation, and even though it seems obvious for NETs to intervene in the disease progression by supporting inflammation, no reliable data are available. We discuss in this review the potential pathophysiological relevance of NET formation in MPNs, with the intention of contributing to a better understanding of how neutrophils and neutrophil clonality can orchestrate the evolution of a pathological microenvironment in MPNs.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms",
number = "5",
pages = "4497",
volume = "24",
doi = "10.3390/ijms24054497"
}

Marković, D., Maslovarić, I., Kovačić, M., Vignjević-Petrinović, S.,& Ilić, V.. (2023). Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 24(5), 4497.
https://doi.org/10.3390/ijms24054497

Marković D, Maslovarić I, Kovačić M, Vignjević-Petrinović S, Ilić V. Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms. in International Journal of Molecular Sciences. 2023;24(5):4497.
doi:10.3390/ijms24054497 .

Marković, Dragana, Maslovarić, Irina, Kovačić, Marijana, Vignjević-Petrinović, Sanja, Ilić, Vesna, "Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms" in International Journal of Molecular Sciences, 24, no. 5 (2023):4497,
https://doi.org/10.3390/ijms24054497 . .

TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Bogdanović, Andrija
AU  - Marković, Dragana
AU  - Mitrović-Ajtić, Olivera
AU  - Subotički, Tijana
AU  - Diklić, Miloš
AU  - Vukotić, Milica
AU  - Dragojević, Teodora
AU  - Živković, Emilija
AU  - Santibanez, Juan F.
AU  - Čokić, Vladan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1202
AB  - Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the pro-liferation-related AKT/mTOR signaling pathway and the relationship with inflammatory factors in chronic myelogenous leukemia (CML). The activity of the antioxidant enzymes superoxide dis-mutase, glutathione peroxidase, and catalase is reduced in erythrocytes while levels of the oxidative stress markers malondialdehyde and protein carbonyl are elevated in the plasma of patients with CML. In addition, nitrogen species (nitrotyrosine, iNOS, eNOS) and inflammation markers (IL-6, NFkB, and S100 protein) were increased in granulocytes of CML while anti-inflammatory levels of IL-10 were decreased in plasma. CML granulocytes exhibited greater resistance to cytotoxic H2O2 activity compared to healthy subjects. Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H2O2) in granulocytes and erythroleukemic K562 cells. IL-6 caused oxidative stress and DNA damage that was mitigated using antioxidant or inhibition of inflammatory NFkB transcription factor in K562 cells. We demonstrated the presence of oxidative and ni-trosative stress in CML, with the former mediated by AKT/mTOR signaling and stimulated by in-flammation.
PB  - MDPI
T2  - Biomolecules
T1  - Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
IS  - 2
SP  - 247
VL  - 12
DO  - 10.3390/biom12020247
ER  - 

@article{
author = "Đikić, Dragoslava and Bogdanović, Andrija and Marković, Dragana and Mitrović-Ajtić, Olivera and Subotički, Tijana and Diklić, Miloš and Vukotić, Milica and Dragojević, Teodora and Živković, Emilija and Santibanez, Juan F. and Čokić, Vladan",
year = "2022",
abstract = "Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the pro-liferation-related AKT/mTOR signaling pathway and the relationship with inflammatory factors in chronic myelogenous leukemia (CML). The activity of the antioxidant enzymes superoxide dis-mutase, glutathione peroxidase, and catalase is reduced in erythrocytes while levels of the oxidative stress markers malondialdehyde and protein carbonyl are elevated in the plasma of patients with CML. In addition, nitrogen species (nitrotyrosine, iNOS, eNOS) and inflammation markers (IL-6, NFkB, and S100 protein) were increased in granulocytes of CML while anti-inflammatory levels of IL-10 were decreased in plasma. CML granulocytes exhibited greater resistance to cytotoxic H2O2 activity compared to healthy subjects. Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H2O2) in granulocytes and erythroleukemic K562 cells. IL-6 caused oxidative stress and DNA damage that was mitigated using antioxidant or inhibition of inflammatory NFkB transcription factor in K562 cells. We demonstrated the presence of oxidative and ni-trosative stress in CML, with the former mediated by AKT/mTOR signaling and stimulated by in-flammation.",
publisher = "MDPI",
journal = "Biomolecules",
title = "Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia",
number = "2",
pages = "247",
volume = "12",
doi = "10.3390/biom12020247"
}

Đikić, D., Bogdanović, A., Marković, D., Mitrović-Ajtić, O., Subotički, T., Diklić, M., Vukotić, M., Dragojević, T., Živković, E., Santibanez, J. F.,& Čokić, V.. (2022). Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia. in Biomolecules
MDPI., 12(2), 247.
https://doi.org/10.3390/biom12020247

Đikić D, Bogdanović A, Marković D, Mitrović-Ajtić O, Subotički T, Diklić M, Vukotić M, Dragojević T, Živković E, Santibanez JF, Čokić V. Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia. in Biomolecules. 2022;12(2):247.
doi:10.3390/biom12020247 .

Đikić, Dragoslava, Bogdanović, Andrija, Marković, Dragana, Mitrović-Ajtić, Olivera, Subotički, Tijana, Diklić, Miloš, Vukotić, Milica, Dragojević, Teodora, Živković, Emilija, Santibanez, Juan F., Čokić, Vladan, "Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia" in Biomolecules, 12, no. 2 (2022):247,
https://doi.org/10.3390/biom12020247 . .

TY  - JOUR
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Đikić, Dragoslava
AU  - Čokić, Vladan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1205
AB  - Neutrophils are an essential component of the innate immune response, but their prolonged activation can lead to chronic inflammation. Consequently, neutrophil homeostasis is tightly regulated through balance between granulopoiesis and clearance of dying cells. The bone marrow is both a site of neutrophil production and the place they return to and die. Myeloproliferative neoplasms (MPN) are clonal hematopoietic disorders characterized by the mutations in three types of molecular markers, with emphasis on Janus kinase 2 gene mutation (JAK2V617F). The MPN bone marrow stem cell niche is a site of chronic inflammation, with commonly increased cells of myeloid lineage, including neutrophils. The MPN neutrophils are characterized by the upregulation of JAK target genes. Additionally, MPN neutrophils display malignant nature, they are in a state of activation, and with deregulated apoptotic machinery. In other words, neutrophils deserve to be placed in the midst of major events in MPN. Our crucial interest in this review is better understanding of how neutrophils die in MPN mirrored by defects in apoptosis and to what possible extent they can contribute to MPN pathophysiology. We tend to expect that reduced neutrophil apoptosis will establish a pathogenic link to chronic inflammation in MPN.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Neutrophil Death in Myeloproliferative Neoplasms: Shedding More Light on Neutrophils as a Pathogenic Link to Chronic Inflammation
IS  - 3
SP  - 1490
VL  - 23
DO  - 10.3390/ijms23031490
ER  - 

@article{
author = "Marković, Dragana and Maslovarić, Irina and Đikić, Dragoslava and Čokić, Vladan",
year = "2022",
abstract = "Neutrophils are an essential component of the innate immune response, but their prolonged activation can lead to chronic inflammation. Consequently, neutrophil homeostasis is tightly regulated through balance between granulopoiesis and clearance of dying cells. The bone marrow is both a site of neutrophil production and the place they return to and die. Myeloproliferative neoplasms (MPN) are clonal hematopoietic disorders characterized by the mutations in three types of molecular markers, with emphasis on Janus kinase 2 gene mutation (JAK2V617F). The MPN bone marrow stem cell niche is a site of chronic inflammation, with commonly increased cells of myeloid lineage, including neutrophils. The MPN neutrophils are characterized by the upregulation of JAK target genes. Additionally, MPN neutrophils display malignant nature, they are in a state of activation, and with deregulated apoptotic machinery. In other words, neutrophils deserve to be placed in the midst of major events in MPN. Our crucial interest in this review is better understanding of how neutrophils die in MPN mirrored by defects in apoptosis and to what possible extent they can contribute to MPN pathophysiology. We tend to expect that reduced neutrophil apoptosis will establish a pathogenic link to chronic inflammation in MPN.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Neutrophil Death in Myeloproliferative Neoplasms: Shedding More Light on Neutrophils as a Pathogenic Link to Chronic Inflammation",
number = "3",
pages = "1490",
volume = "23",
doi = "10.3390/ijms23031490"
}

Marković, D., Maslovarić, I., Đikić, D.,& Čokić, V.. (2022). Neutrophil Death in Myeloproliferative Neoplasms: Shedding More Light on Neutrophils as a Pathogenic Link to Chronic Inflammation. in International Journal of Molecular Sciences
MDPI., 23(3), 1490.
https://doi.org/10.3390/ijms23031490

Marković D, Maslovarić I, Đikić D, Čokić V. Neutrophil Death in Myeloproliferative Neoplasms: Shedding More Light on Neutrophils as a Pathogenic Link to Chronic Inflammation. in International Journal of Molecular Sciences. 2022;23(3):1490.
doi:10.3390/ijms23031490 .

Marković, Dragana, Maslovarić, Irina, Đikić, Dragoslava, Čokić, Vladan, "Neutrophil Death in Myeloproliferative Neoplasms: Shedding More Light on Neutrophils as a Pathogenic Link to Chronic Inflammation" in International Journal of Molecular Sciences, 23, no. 3 (2022):1490,
https://doi.org/10.3390/ijms23031490 . .

TY  - JOUR
AU  - Šefer, Dijana
AU  - Miljić, Predrag
AU  - Kraguljac-Kurtović, Nada
AU  - Bižić-Radulović, Sandra
AU  - Bogdanović, Andrija
AU  - Knežević, Vesna
AU  - Marković, Dragana
AU  - Beleslin-Čokić, Bojana
AU  - Novaković, Ivana
AU  - Marinković, Jelena
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Čokić, Vladan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1231
AB  - Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA.
T2  - International Journal of Laboratory Hematology
T1  - Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms
EP  - 312
IS  - 2
SP  - 302
VL  - 44
DO  - 10.1111/ijlh.13754
ER  - 

@article{
author = "Šefer, Dijana and Miljić, Predrag and Kraguljac-Kurtović, Nada and Bižić-Radulović, Sandra and Bogdanović, Andrija and Knežević, Vesna and Marković, Dragana and Beleslin-Čokić, Bojana and Novaković, Ivana and Marinković, Jelena and Leković, Danijela and Gotić, Mirjana and Čokić, Vladan",
year = "2022",
abstract = "Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA.",
journal = "International Journal of Laboratory Hematology",
title = "Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms",
pages = "312-302",
number = "2",
volume = "44",
doi = "10.1111/ijlh.13754"
}

Šefer, D., Miljić, P., Kraguljac-Kurtović, N., Bižić-Radulović, S., Bogdanović, A., Knežević, V., Marković, D., Beleslin-Čokić, B., Novaković, I., Marinković, J., Leković, D., Gotić, M.,& Čokić, V.. (2022). Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms. in International Journal of Laboratory Hematology, 44(2), 302-312.
https://doi.org/10.1111/ijlh.13754

Šefer D, Miljić P, Kraguljac-Kurtović N, Bižić-Radulović S, Bogdanović A, Knežević V, Marković D, Beleslin-Čokić B, Novaković I, Marinković J, Leković D, Gotić M, Čokić V. Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms. in International Journal of Laboratory Hematology. 2022;44(2):302-312.
doi:10.1111/ijlh.13754 .

Šefer, Dijana, Miljić, Predrag, Kraguljac-Kurtović, Nada, Bižić-Radulović, Sandra, Bogdanović, Andrija, Knežević, Vesna, Marković, Dragana, Beleslin-Čokić, Bojana, Novaković, Ivana, Marinković, Jelena, Leković, Danijela, Gotić, Mirjana, Čokić, Vladan, "Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms" in International Journal of Laboratory Hematology, 44, no. 2 (2022):302-312,
https://doi.org/10.1111/ijlh.13754 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Grujić-Milanović, Jelica
AU  - Vejnović, Branislav
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1237
AB  - Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.
PB  - Taylor & Francis
T2  - Italian Journal of Animal Science
T1  - Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows
EP  - 858
IS  - 1
SP  - 847
VL  - 21
DO  - 10.1080/1828051X.2022.2070034
ER  - 

@article{
author = "Drvenica, Ivana and Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Grujić-Milanović, Jelica and Vejnović, Branislav and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna",
year = "2022",
abstract = "Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.",
publisher = "Taylor & Francis",
journal = "Italian Journal of Animal Science",
title = "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows",
pages = "858-847",
number = "1",
volume = "21",
doi = "10.1080/1828051X.2022.2070034"
}

Drvenica, I., Stojić, M., Fratrić, N., Kovačić, M., Grujić-Milanović, J., Vejnović, B., Marković, D., Gvozdić, D.,& Ilić, V.. (2022). Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science
Taylor & Francis., 21(1), 847-858.
https://doi.org/10.1080/1828051X.2022.2070034

Drvenica I, Stojić M, Fratrić N, Kovačić M, Grujić-Milanović J, Vejnović B, Marković D, Gvozdić D, Ilić V. Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science. 2022;21(1):847-858.
doi:10.1080/1828051X.2022.2070034 .

Drvenica, Ivana, Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Grujić-Milanović, Jelica, Vejnović, Branislav, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows" in Italian Journal of Animal Science, 21, no. 1 (2022):847-858,
https://doi.org/10.1080/1828051X.2022.2070034 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Stančić, Ana
AU  - Marković, Dragana
AU  - Kovačić, Marijana
AU  - Maslovarić, Irina
AU  - Rapajić, Ivana
AU  - Vučetić, Dušan
AU  - Ilić, Vesna
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1176
AB  - Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.
PB  - Springer Nature
T2  - European Biophysics Journal
T1  - The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method
EP  - 846
IS  - 6
SP  - 829
VL  - 50
DO  - 10.1007/s00249-021-01527-3
ER  - 

@article{
author = "Drvenica, Ivana and Mojsilović, Slavko and Stančić, Ana and Marković, Dragana and Kovačić, Marijana and Maslovarić, Irina and Rapajić, Ivana and Vučetić, Dušan and Ilić, Vesna",
year = "2021",
abstract = "Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.",
publisher = "Springer Nature",
journal = "European Biophysics Journal",
title = "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method",
pages = "846-829",
number = "6",
volume = "50",
doi = "10.1007/s00249-021-01527-3"
}

Drvenica, I., Mojsilović, S., Stančić, A., Marković, D., Kovačić, M., Maslovarić, I., Rapajić, I., Vučetić, D.,& Ilić, V.. (2021). The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal
Springer Nature., 50(6), 829-846.
https://doi.org/10.1007/s00249-021-01527-3

Drvenica I, Mojsilović S, Stančić A, Marković D, Kovačić M, Maslovarić I, Rapajić I, Vučetić D, Ilić V. The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal. 2021;50(6):829-846.
doi:10.1007/s00249-021-01527-3 .

Drvenica, Ivana, Mojsilović, Slavko, Stančić, Ana, Marković, Dragana, Kovačić, Marijana, Maslovarić, Irina, Rapajić, Ivana, Vučetić, Dušan, Ilić, Vesna, "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method" in European Biophysics Journal, 50, no. 6 (2021):829-846,
https://doi.org/10.1007/s00249-021-01527-3 . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Repac, Jelena
AU  - Božić, Bojan
AU  - Radulović, Željko
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Ristanović, Elizabeta
AU  - Chochlakis, Dimosthenis
AU  - Nedeljković Božić, Biljana 
AU  - Tomanović, Snežana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1160
AB  - In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.
PB  - Springer
T2  - Experimental and Applied Acarology
T1  - Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization
EP  - 99
IS  - 1
SP  - 83
VL  - 85
DO  - 10.1007/s10493-021-00653-z
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Repac, Jelena and Božić, Bojan and Radulović, Željko and Veinović, Gorana and Sukara, Ratko and Ristanović, Elizabeta and Chochlakis, Dimosthenis and Nedeljković Božić, Biljana  and Tomanović, Snežana",
year = "2021",
abstract = "In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.",
publisher = "Springer",
journal = "Experimental and Applied Acarology",
title = "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization",
pages = "99-83",
number = "1",
volume = "85",
doi = "10.1007/s10493-021-00653-z"
}

Mihaljica, D., Marković, D., Repac, J., Božić, B., Radulović, Ž., Veinović, G., Sukara, R., Ristanović, E., Chochlakis, D., Nedeljković Božić, B.,& Tomanović, S.. (2021). Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology
Springer., 85(1), 83-99.
https://doi.org/10.1007/s10493-021-00653-z

Mihaljica D, Marković D, Repac J, Božić B, Radulović Ž, Veinović G, Sukara R, Ristanović E, Chochlakis D, Nedeljković Božić B, Tomanović S. Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology. 2021;85(1):83-99.
doi:10.1007/s10493-021-00653-z .

Mihaljica, Darko, Marković, Dragana, Repac, Jelena, Božić, Bojan, Radulović, Željko, Veinović, Gorana, Sukara, Ratko, Ristanović, Elizabeta, Chochlakis, Dimosthenis, Nedeljković Božić, Biljana , Tomanović, Snežana, "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization" in Experimental and Applied Acarology, 85, no. 1 (2021):83-99,
https://doi.org/10.1007/s10493-021-00653-z . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Fratrić, Natalija
AU  - Arsić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Drvenica, Ivana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Ilić, Vesna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1010
AB  - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
EP  - 74
SP  - 63
VL  - 133
DO  - 10.1016/j.rvsc.2020.09.004
ER  - 

@article{
author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić-Milanović, Jelica and Ivanov, Milan and Ilić, Vesna",
year = "2020",
abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes",
pages = "74-63",
volume = "133",
doi = "10.1016/j.rvsc.2020.09.004"
}

Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić-Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 133, 63-74.
https://doi.org/10.1016/j.rvsc.2020.09.004

Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić-Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74.
doi:10.1016/j.rvsc.2020.09.004 .

Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić-Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74,
https://doi.org/10.1016/j.rvsc.2020.09.004 . .

TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Mitrović-Ajtić, Olivera
AU  - Jovčić, Gordana
AU  - Milošević, Maja
AU  - Momčilović, Sanja
AU  - Čokić, Vladan
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1029
AB  - Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress exposure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients.
PB  - Springer, New York
T2  - Histochemistry & Cell Biology
T1  - Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress
EP  - 468
IS  - 6
SP  - 457
VL  - 153
DO  - 10.1007/s00418-020-01856-y
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Budeč, Mirela and Marković, Dragana and Mitrović-Ajtić, Olivera and Jovčić, Gordana and Milošević, Maja and Momčilović, Sanja and Čokić, Vladan",
year = "2020",
abstract = "Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress exposure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients.",
publisher = "Springer, New York",
journal = "Histochemistry & Cell Biology",
title = "Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress",
pages = "468-457",
number = "6",
volume = "153",
doi = "10.1007/s00418-020-01856-y"
}

Vignjević-Petrinović, S., Budeč, M., Marković, D., Mitrović-Ajtić, O., Jovčić, G., Milošević, M., Momčilović, S.,& Čokić, V.. (2020). Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress. in Histochemistry & Cell Biology
Springer, New York., 153(6), 457-468.
https://doi.org/10.1007/s00418-020-01856-y

Vignjević-Petrinović S, Budeč M, Marković D, Mitrović-Ajtić O, Jovčić G, Milošević M, Momčilović S, Čokić V. Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress. in Histochemistry & Cell Biology. 2020;153(6):457-468.
doi:10.1007/s00418-020-01856-y .

Vignjević-Petrinović, Sanja, Budeč, Mirela, Marković, Dragana, Mitrović-Ajtić, Olivera, Jovčić, Gordana, Milošević, Maja, Momčilović, Sanja, Čokić, Vladan, "Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress" in Histochemistry & Cell Biology, 153, no. 6 (2020):457-468,
https://doi.org/10.1007/s00418-020-01856-y . .

TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Marković, Dragana
AU  - Bogdanović, Andrija
AU  - Mitrović-Ajtić, Olivera
AU  - Subotički, Tijana
AU  - Diklić, Miloš
AU  - Beleslin-Čokić, Bojana
AU  - Bjelica, Sunčica
AU  - Kovačić, Marijana
AU  - Čokić, Vladan
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/836
AB  - Purpose: A common feature of malignancies is increased reactive oxygen species (ROS) and reactive nitrogen species (RNS). We analyzed the influence of oxidative and nitrosative stress on the activation of AKT/mTOR signaling pathway in myeloproliferative neoplasms (MPN). Methods: Oxidative stress-induced gene expression in circulatory CD34(+) cells of MPN patients was studied by microarray analysis. Biomarkers of oxidative and nitrosative stress were determined using spectrophotometry in plasma and erythrocyte lysate. The levels of nitrotyrosine, inducible NO synthase (iNOS) and AKT/mTOR/p70S6K phosphorylation were determined by immunocytochemistry and immunoblotting in granulocytes of MPN patients. Results: Antioxidants superoxide dismutase 2 (SOD2) and glutathione peroxidase 1 (GPx1) gene expression were increased in circulatory CD34(+) cells, while SODI and GPx enzymes were reduced in the erythrocytes of MPN. Plasma malonyl-dialdehyde and protein carbonyl levels were elevated in MPN. The total antioxidant capacity in plasma and erythrocyte catalase (CT) activities was the most prominent in primary myelofibrosis (PMF) with JAK2V617F heterozygosity. The total nitrite/ nitrate (NOx) level was augmented in the plasma of PMF patients (p  lt 0.001), while nitrotyrosine and iNOS were generally increased in the granulocytes of MPN patients. Activation of AKT/m TOR signaling was the most significant in PMF (p lt 0.01), but demonstrated JAK2V617F dependence and consequent p70S6K phosphorylation in the granulocytes of essential thrombocytemia (ET) and polycythemia vera (PV) patients. Hydrogen peroxide stimulated mTOR pathway, iNOS and nitrotyrosine quantities, the last one prevented by the antioxidant nacetyl-cysteine (NAC) in the granulocytes of MPN. Conclusion: Our study showed increased levels of oxidative and nitrosative stress parameters in MPN with JAK2V617F dependence. The ROS enhanced the constitutive activation of AKT/mTOR signaling and nitrosative parameters in MPN.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway
EP  - 1491
IS  - 5
SP  - 1481
VL  - 23
UR  - https://hdl.handle.net/21.15107/rcub_rimi_836
ER  - 

@article{
author = "Đikić, Dragoslava and Marković, Dragana and Bogdanović, Andrija and Mitrović-Ajtić, Olivera and Subotički, Tijana and Diklić, Miloš and Beleslin-Čokić, Bojana and Bjelica, Sunčica and Kovačić, Marijana and Čokić, Vladan",
year = "2018",
abstract = "Purpose: A common feature of malignancies is increased reactive oxygen species (ROS) and reactive nitrogen species (RNS). We analyzed the influence of oxidative and nitrosative stress on the activation of AKT/mTOR signaling pathway in myeloproliferative neoplasms (MPN). Methods: Oxidative stress-induced gene expression in circulatory CD34(+) cells of MPN patients was studied by microarray analysis. Biomarkers of oxidative and nitrosative stress were determined using spectrophotometry in plasma and erythrocyte lysate. The levels of nitrotyrosine, inducible NO synthase (iNOS) and AKT/mTOR/p70S6K phosphorylation were determined by immunocytochemistry and immunoblotting in granulocytes of MPN patients. Results: Antioxidants superoxide dismutase 2 (SOD2) and glutathione peroxidase 1 (GPx1) gene expression were increased in circulatory CD34(+) cells, while SODI and GPx enzymes were reduced in the erythrocytes of MPN. Plasma malonyl-dialdehyde and protein carbonyl levels were elevated in MPN. The total antioxidant capacity in plasma and erythrocyte catalase (CT) activities was the most prominent in primary myelofibrosis (PMF) with JAK2V617F heterozygosity. The total nitrite/ nitrate (NOx) level was augmented in the plasma of PMF patients (p  lt 0.001), while nitrotyrosine and iNOS were generally increased in the granulocytes of MPN patients. Activation of AKT/m TOR signaling was the most significant in PMF (p lt 0.01), but demonstrated JAK2V617F dependence and consequent p70S6K phosphorylation in the granulocytes of essential thrombocytemia (ET) and polycythemia vera (PV) patients. Hydrogen peroxide stimulated mTOR pathway, iNOS and nitrotyrosine quantities, the last one prevented by the antioxidant nacetyl-cysteine (NAC) in the granulocytes of MPN. Conclusion: Our study showed increased levels of oxidative and nitrosative stress parameters in MPN with JAK2V617F dependence. The ROS enhanced the constitutive activation of AKT/mTOR signaling and nitrosative parameters in MPN.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway",
pages = "1491-1481",
number = "5",
volume = "23",
url = "https://hdl.handle.net/21.15107/rcub_rimi_836"
}

Đikić, D., Marković, D., Bogdanović, A., Mitrović-Ajtić, O., Subotički, T., Diklić, M., Beleslin-Čokić, B., Bjelica, S., Kovačić, M.,& Čokić, V.. (2018). Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(5), 1481-1491.
https://hdl.handle.net/21.15107/rcub_rimi_836

Đikić D, Marković D, Bogdanović A, Mitrović-Ajtić O, Subotički T, Diklić M, Beleslin-Čokić B, Bjelica S, Kovačić M, Čokić V. Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway. in Journal of BUON. 2018;23(5):1481-1491.
https://hdl.handle.net/21.15107/rcub_rimi_836 .

Đikić, Dragoslava, Marković, Dragana, Bogdanović, Andrija, Mitrović-Ajtić, Olivera, Subotički, Tijana, Diklić, Miloš, Beleslin-Čokić, Bojana, Bjelica, Sunčica, Kovačić, Marijana, Čokić, Vladan, "Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway" in Journal of BUON, 23, no. 5 (2018):1481-1491,
https://hdl.handle.net/21.15107/rcub_rimi_836 .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Stojković, Aleksandra
AU  - Kosanović, Dejana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Jovanova-Nešić, Katica
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/840
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis
T1  - Vakcinacija i autoimunost - vakcina protiv gripa i povezanost sa multiplom sklerozom
EP  - 729
IS  - 7
SP  - 721
VL  - 75
DO  - 10.2298/VSP160620002M
ER  - 

@article{
author = "Maslovarić, Irina and Stojković, Aleksandra and Kosanović, Dejana and Marković, Dragana and Ilić, Vesna and Jovanova-Nešić, Katica",
year = "2018",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis, Vakcinacija i autoimunost - vakcina protiv gripa i povezanost sa multiplom sklerozom",
pages = "729-721",
number = "7",
volume = "75",
doi = "10.2298/VSP160620002M"
}

Maslovarić, I., Stojković, A., Kosanović, D., Marković, D., Ilić, V.,& Jovanova-Nešić, K.. (2018). Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 75(7), 721-729.
https://doi.org/10.2298/VSP160620002M

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis. in Vojnosanitetski pregled. 2018;75(7):721-729.
doi:10.2298/VSP160620002M .

Maslovarić, Irina, Stojković, Aleksandra, Kosanović, Dejana, Marković, Dragana, Ilić, Vesna, Jovanova-Nešić, Katica, "Vaccination and autoimmunity: Influenza vaccination and association with multiple sclerosis" in Vojnosanitetski pregled, 75, no. 7 (2018):721-729,
https://doi.org/10.2298/VSP160620002M . .

TY  - JOUR
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Skoda, Radek C.
AU  - Beleslin-Čokić, Bojana
AU  - Milić, Nataša
AU  - Mitrović-Ajtić, Olivera
AU  - Nienhold, Ronny
AU  - Sefer, Dijana
AU  - Subotički, Tijana
AU  - Kovačić, Marijana
AU  - Marković, Dragana
AU  - Diklić, Miloš
AU  - Čokić, Vladan
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/804
AB  - Increased angiogenesis in BCR-ABL1 negative myeloproliferative neoplasms (MPNs) has been recognized, but its connection with clinical and molecular markers needs to be defined. The aims of study were to (1) assess bone marrow (BM) angiogenesis measured by microvessel density (MVD) using CD34 and CD105 antibodies; (2) analyze correlation of MVD with plasma angiogenic factors including vascular endothelial growth factor, basic fibroblast growth factor, and interleukin-8; (3) examine the association of MVD with clinicopathological and molecular markers. We examined 90 de novo MPN patients (30 polycythemia vera (PV), primary myelofibrosis (PMF), essential thrombocythemia (ET)) and 10 age-matched controls. MVD was analyzed by immunohistochemistry "hot spot" method, angiogenic factors by immunoassay and JAK2V617F, and CALR mutations by DNA sequencing and allelic PCR. MVD was significantly increased in MPNs compared to controls (PMF  gt  PV  gt  ET). Correlation between MVD and plasma angiogenic factors was found in MPNs. MVD was significantly increased in patients with JAK2V617F mutation and correlated with JAK2 mutant allele burden (CD34-MVD: rho = 0.491, p  lt  0.001; CD105-MVD: rho = 0.276, p = 0.02) but not with CALR mutation. MVD correlated with leukocyte count, serum lactate dehydrogenase, hepatomegaly, and splenomegaly. BM fibrosis was significantly associated with CD34-MVD, CD105-MVD, interleukin-8, and JAK2 mutant allele burden. JAK2 homozygote status had positive predictive value (100%) for BM fibrosis. Patients with prefibrotic PMF had significantly higher MVD than patients with ET, and we could recommend MVD to be additional histopathological marker to distinguish these two entities. This study also highlights the strong correlation of MVD with plasma angiogenic factors, JAK2 mutant allele burden, and BM fibrosis in MPNs.
PB  - Springer, New York
T2  - Annals of Hematology
T1  - Bone marrow microvessel density and plasma angiogenic factors in myeloproliferative neoplasms: clinicopathological and molecular correlations
EP  - 404
IS  - 3
SP  - 393
VL  - 96
DO  - 10.1007/s00277-016-2890-9
ER  - 

@article{
author = "Leković, Danijela and Gotić, Mirjana and Skoda, Radek C. and Beleslin-Čokić, Bojana and Milić, Nataša and Mitrović-Ajtić, Olivera and Nienhold, Ronny and Sefer, Dijana and Subotički, Tijana and Kovačić, Marijana and Marković, Dragana and Diklić, Miloš and Čokić, Vladan",
year = "2017",
abstract = "Increased angiogenesis in BCR-ABL1 negative myeloproliferative neoplasms (MPNs) has been recognized, but its connection with clinical and molecular markers needs to be defined. The aims of study were to (1) assess bone marrow (BM) angiogenesis measured by microvessel density (MVD) using CD34 and CD105 antibodies; (2) analyze correlation of MVD with plasma angiogenic factors including vascular endothelial growth factor, basic fibroblast growth factor, and interleukin-8; (3) examine the association of MVD with clinicopathological and molecular markers. We examined 90 de novo MPN patients (30 polycythemia vera (PV), primary myelofibrosis (PMF), essential thrombocythemia (ET)) and 10 age-matched controls. MVD was analyzed by immunohistochemistry "hot spot" method, angiogenic factors by immunoassay and JAK2V617F, and CALR mutations by DNA sequencing and allelic PCR. MVD was significantly increased in MPNs compared to controls (PMF  gt  PV  gt  ET). Correlation between MVD and plasma angiogenic factors was found in MPNs. MVD was significantly increased in patients with JAK2V617F mutation and correlated with JAK2 mutant allele burden (CD34-MVD: rho = 0.491, p  lt  0.001; CD105-MVD: rho = 0.276, p = 0.02) but not with CALR mutation. MVD correlated with leukocyte count, serum lactate dehydrogenase, hepatomegaly, and splenomegaly. BM fibrosis was significantly associated with CD34-MVD, CD105-MVD, interleukin-8, and JAK2 mutant allele burden. JAK2 homozygote status had positive predictive value (100%) for BM fibrosis. Patients with prefibrotic PMF had significantly higher MVD than patients with ET, and we could recommend MVD to be additional histopathological marker to distinguish these two entities. This study also highlights the strong correlation of MVD with plasma angiogenic factors, JAK2 mutant allele burden, and BM fibrosis in MPNs.",
publisher = "Springer, New York",
journal = "Annals of Hematology",
title = "Bone marrow microvessel density and plasma angiogenic factors in myeloproliferative neoplasms: clinicopathological and molecular correlations",
pages = "404-393",
number = "3",
volume = "96",
doi = "10.1007/s00277-016-2890-9"
}

Leković, D., Gotić, M., Skoda, R. C., Beleslin-Čokić, B., Milić, N., Mitrović-Ajtić, O., Nienhold, R., Sefer, D., Subotički, T., Kovačić, M., Marković, D., Diklić, M.,& Čokić, V.. (2017). Bone marrow microvessel density and plasma angiogenic factors in myeloproliferative neoplasms: clinicopathological and molecular correlations. in Annals of Hematology
Springer, New York., 96(3), 393-404.
https://doi.org/10.1007/s00277-016-2890-9

Leković D, Gotić M, Skoda RC, Beleslin-Čokić B, Milić N, Mitrović-Ajtić O, Nienhold R, Sefer D, Subotički T, Kovačić M, Marković D, Diklić M, Čokić V. Bone marrow microvessel density and plasma angiogenic factors in myeloproliferative neoplasms: clinicopathological and molecular correlations. in Annals of Hematology. 2017;96(3):393-404.
doi:10.1007/s00277-016-2890-9 .

Leković, Danijela, Gotić, Mirjana, Skoda, Radek C., Beleslin-Čokić, Bojana, Milić, Nataša, Mitrović-Ajtić, Olivera, Nienhold, Ronny, Sefer, Dijana, Subotički, Tijana, Kovačić, Marijana, Marković, Dragana, Diklić, Miloš, Čokić, Vladan, "Bone marrow microvessel density and plasma angiogenic factors in myeloproliferative neoplasms: clinicopathological and molecular correlations" in Annals of Hematology, 96, no. 3 (2017):393-404,
https://doi.org/10.1007/s00277-016-2890-9 . .

TY  - JOUR
AU  - Subotički, Tijana
AU  - Mitrović-Ajtić, Olivera
AU  - Beleslin-Čokić, Bojana
AU  - Nienhold, Ronny
AU  - Diklić, Miloš
AU  - Đikić, Dragoslava
AU  - Leković, Danijela
AU  - Bulat, Tanja
AU  - Marković, Dragana
AU  - Gotić, Mirjana
AU  - Noguchi, Constance T.
AU  - Schechter, Alan N.
AU  - Skoda, Radek C.
AU  - Čokić, Vladan
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/807
AB  - It has been shown that angiogenesis and inflammation play an important role in development of most hematological malignancies including the myeloproliferative neoplasm (MPN). The aim of this study was to investigate and correlate the levels of key angiogenic molecules such as hypoxia-inducible factor-1 (HIF-1), vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in peripheral blood and bone marrow cells of MPN patients, along with JAK2V617F mutation allele burden and effects of therapy. HIF-1 and VEGF gene expression were decreased, while eNOS mRNA levels were increased in granulocytes of MPN patients. Furthermore, positively correlated and increased VEGF and eNOS protein levels were in negative correlation with HIF-1 levels in granulocytes of MPN patients. According to immunoblotting, the generally augmented angiogenic factors demonstrated JAK2V617F allele burden dependence only in granulocytes of PMF. The angiogenic factors were largely reduced after hydroxyurea therapy in granulocytes of MPN patients. Levels of eNOS protein expression were stimulated by Calreticulin mutations in granulocytes of essential thrombocythemia. Immunocytochemical analyses of CD34(+) cells showed a more pronounced enhancement of angiogenic factors than in granulocytes. Increased gene expression linked to the proinflammatory TGF and MAPK signaling pathways were detected in CD34(+) cells of MPN patients. In conclusion, the angiogenesis is increased in several cell types of MPN patients supported by the transcriptional activation of inflammation-related target genes, and is not limited to bone marrow stroma cells. It also appears that some of the benefit of hydroxyurea therapy of the MPN is mediated by effects on angiogenic factors.
PB  - Wiley-Blackwell, Hoboken
T2  - Molecular Carcinogenesis
T1  - Angiogenic factors are increased in circulating granulocytes and CD34(+) cells of myeloproliferative neoplasms
EP  - 579
IS  - 2
SP  - 567
VL  - 56
DO  - 10.1002/mc.22517
ER  - 

@article{
author = "Subotički, Tijana and Mitrović-Ajtić, Olivera and Beleslin-Čokić, Bojana and Nienhold, Ronny and Diklić, Miloš and Đikić, Dragoslava and Leković, Danijela and Bulat, Tanja and Marković, Dragana and Gotić, Mirjana and Noguchi, Constance T. and Schechter, Alan N. and Skoda, Radek C. and Čokić, Vladan",
year = "2017",
abstract = "It has been shown that angiogenesis and inflammation play an important role in development of most hematological malignancies including the myeloproliferative neoplasm (MPN). The aim of this study was to investigate and correlate the levels of key angiogenic molecules such as hypoxia-inducible factor-1 (HIF-1), vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS) in peripheral blood and bone marrow cells of MPN patients, along with JAK2V617F mutation allele burden and effects of therapy. HIF-1 and VEGF gene expression were decreased, while eNOS mRNA levels were increased in granulocytes of MPN patients. Furthermore, positively correlated and increased VEGF and eNOS protein levels were in negative correlation with HIF-1 levels in granulocytes of MPN patients. According to immunoblotting, the generally augmented angiogenic factors demonstrated JAK2V617F allele burden dependence only in granulocytes of PMF. The angiogenic factors were largely reduced after hydroxyurea therapy in granulocytes of MPN patients. Levels of eNOS protein expression were stimulated by Calreticulin mutations in granulocytes of essential thrombocythemia. Immunocytochemical analyses of CD34(+) cells showed a more pronounced enhancement of angiogenic factors than in granulocytes. Increased gene expression linked to the proinflammatory TGF and MAPK signaling pathways were detected in CD34(+) cells of MPN patients. In conclusion, the angiogenesis is increased in several cell types of MPN patients supported by the transcriptional activation of inflammation-related target genes, and is not limited to bone marrow stroma cells. It also appears that some of the benefit of hydroxyurea therapy of the MPN is mediated by effects on angiogenic factors.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Molecular Carcinogenesis",
title = "Angiogenic factors are increased in circulating granulocytes and CD34(+) cells of myeloproliferative neoplasms",
pages = "579-567",
number = "2",
volume = "56",
doi = "10.1002/mc.22517"
}

Subotički, T., Mitrović-Ajtić, O., Beleslin-Čokić, B., Nienhold, R., Diklić, M., Đikić, D., Leković, D., Bulat, T., Marković, D., Gotić, M., Noguchi, C. T., Schechter, A. N., Skoda, R. C.,& Čokić, V.. (2017). Angiogenic factors are increased in circulating granulocytes and CD34(+) cells of myeloproliferative neoplasms. in Molecular Carcinogenesis
Wiley-Blackwell, Hoboken., 56(2), 567-579.
https://doi.org/10.1002/mc.22517

Subotički T, Mitrović-Ajtić O, Beleslin-Čokić B, Nienhold R, Diklić M, Đikić D, Leković D, Bulat T, Marković D, Gotić M, Noguchi CT, Schechter AN, Skoda RC, Čokić V. Angiogenic factors are increased in circulating granulocytes and CD34(+) cells of myeloproliferative neoplasms. in Molecular Carcinogenesis. 2017;56(2):567-579.
doi:10.1002/mc.22517 .

Subotički, Tijana, Mitrović-Ajtić, Olivera, Beleslin-Čokić, Bojana, Nienhold, Ronny, Diklić, Miloš, Đikić, Dragoslava, Leković, Danijela, Bulat, Tanja, Marković, Dragana, Gotić, Mirjana, Noguchi, Constance T., Schechter, Alan N., Skoda, Radek C., Čokić, Vladan, "Angiogenic factors are increased in circulating granulocytes and CD34(+) cells of myeloproliferative neoplasms" in Molecular Carcinogenesis, 56, no. 2 (2017):567-579,
https://doi.org/10.1002/mc.22517 . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Željko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Milanović, Zorana
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/762
AB  - Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts
EP  - 437
IS  - 4
SP  - 429
VL  - 72
DO  - 10.1007/s10493-017-0170-6
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Željko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Milanović, Zorana and Tomanović, Snežana",
year = "2017",
abstract = "Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts",
pages = "437-429",
number = "4",
volume = "72",
doi = "10.1007/s10493-017-0170-6"
}

Mihaljica, D., Marković, D., Radulović, Ž., Mulenga, A., Ćakić, S., Sukara, R., Milanović, Z.,& Tomanović, S.. (2017). Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology
Springer, Dordrecht., 72(4), 429-437.
https://doi.org/10.1007/s10493-017-0170-6

Mihaljica D, Marković D, Radulović Ž, Mulenga A, Ćakić S, Sukara R, Milanović Z, Tomanović S. Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology. 2017;72(4):429-437.
doi:10.1007/s10493-017-0170-6 .

Mihaljica, Darko, Marković, Dragana, Radulović, Željko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Milanović, Zorana, Tomanović, Snežana, "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts" in Experimental & Applied Acarology, 72, no. 4 (2017):429-437,
https://doi.org/10.1007/s10493-017-0170-6 . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Eljko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Samardzić, Jelena
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/816
AB  - Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks & Tick-Borne Diseases
T1  - Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation
EP  - 395
IS  - 3
SP  - 391
VL  - 8
DO  - 10.1016/j.ttbdis.2017.01.001
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Eljko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Samardzić, Jelena and Tomanović, Snežana",
year = "2017",
abstract = "Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks & Tick-Borne Diseases",
title = "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation",
pages = "395-391",
number = "3",
volume = "8",
doi = "10.1016/j.ttbdis.2017.01.001"
}

Mihaljica, D., Marković, D., Radulović, E., Mulenga, A., Ćakić, S., Sukara, R., Samardzić, J.,& Tomanović, S.. (2017). Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 8(3), 391-395.
https://doi.org/10.1016/j.ttbdis.2017.01.001

Mihaljica D, Marković D, Radulović E, Mulenga A, Ćakić S, Sukara R, Samardzić J, Tomanović S. Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases. 2017;8(3):391-395.
doi:10.1016/j.ttbdis.2017.01.001 .

Mihaljica, Darko, Marković, Dragana, Radulović, Eljko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Samardzić, Jelena, Tomanović, Snežana, "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation" in Ticks & Tick-Borne Diseases, 8, no. 3 (2017):391-395,
https://doi.org/10.1016/j.ttbdis.2017.01.001 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Stojković, A.
AU  - Kosanović, Dejana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Jovanova-Nešić, Katica
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/775
AB  - The names of the authors were indicated incorrectly (wrong using of diacritical marks). The names of the authors should be written and read as follows: I. MaslovariAc, A. StojkoviAc, D. KosanoviAc, D. MarkoviAc, V. IliAc, and K. Jovanova-NeiAc.
PB  - Springer, New York
T2  - Neurophysiology
T1  - Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (vol 49, pg 276, 2017)
EP  - 393
IS  - 5
SP  - 393
VL  - 49
DO  - 10.1007/s11062-018-9699-1
ER  - 

@article{
author = "Maslovarić, Irina and Stojković, A. and Kosanović, Dejana and Marković, Dragana and Ilić, Vesna and Jovanova-Nešić, Katica",
year = "2017",
abstract = "The names of the authors were indicated incorrectly (wrong using of diacritical marks). The names of the authors should be written and read as follows: I. MaslovariAc, A. StojkoviAc, D. KosanoviAc, D. MarkoviAc, V. IliAc, and K. Jovanova-NeiAc.",
publisher = "Springer, New York",
journal = "Neurophysiology",
title = "Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (vol 49, pg 276, 2017)",
pages = "393-393",
number = "5",
volume = "49",
doi = "10.1007/s11062-018-9699-1"
}

Maslovarić, I., Stojković, A., Kosanović, D., Marković, D., Ilić, V.,& Jovanova-Nešić, K.. (2017). Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (vol 49, pg 276, 2017). in Neurophysiology
Springer, New York., 49(5), 393-393.
https://doi.org/10.1007/s11062-018-9699-1

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (vol 49, pg 276, 2017). in Neurophysiology. 2017;49(5):393-393.
doi:10.1007/s11062-018-9699-1 .

Maslovarić, Irina, Stojković, A., Kosanović, Dejana, Marković, Dragana, Ilić, Vesna, Jovanova-Nešić, Katica, "Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus (vol 49, pg 276, 2017)" in Neurophysiology, 49, no. 5 (2017):393-393,
https://doi.org/10.1007/s11062-018-9699-1 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Stojković, A.
AU  - Kosanović, Dejana
AU  - Marković, Dragana
AU  - Ilić, Vesna
AU  - Jovanova-Nešić, Katica
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/787
AB  - We examined the accessibility of influenza virus and diphtheria-tetanus toxin (DiTe) antigens to the choroid plexus (CP) within the postvaccination period and the expression of CD11b molecules (by immunohistochemistry). Eighteen Dark Agouti (DA) rats were divided into three groups: (i) animals administered with influenza vaccine (Flu), (ii) animals administered with DiTe vaccine (DiTe), and (iii) nontreated (Contr) animals. The serum antibody titers following influenza and diphtheria-tetanus vaccination were detected by the ELISA test. Immunohistochemical staining revealed a great number of viral antigen-positive and CD11b-positive brain cells in Flu rats compared to a very small number of the respective cells in DiTe animals and no staining in the Contr group. DiTe- and Flu-rats showed significant increases in the serum anti-tetanus toxoid and anti-influenza virus antibody levels compared to those in the Contr group. The results obtained attract attention towards the dynamic role of the CP in the immunosurveillance of the CNS. Based on the viral antigen deposits accumulated in the CP, it has been proposed that the latter can play an active role in modulation of the immune response after influenza vaccine immunization.
PB  - Springer, New York
T2  - Neurophysiology
T1  - Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus
EP  - 282
IS  - 4
SP  - 276
VL  - 49
DO  - 10.1007/s11062-017-9682-2
ER  - 

@article{
author = "Maslovarić, Irina and Stojković, A. and Kosanović, Dejana and Marković, Dragana and Ilić, Vesna and Jovanova-Nešić, Katica",
year = "2017",
abstract = "We examined the accessibility of influenza virus and diphtheria-tetanus toxin (DiTe) antigens to the choroid plexus (CP) within the postvaccination period and the expression of CD11b molecules (by immunohistochemistry). Eighteen Dark Agouti (DA) rats were divided into three groups: (i) animals administered with influenza vaccine (Flu), (ii) animals administered with DiTe vaccine (DiTe), and (iii) nontreated (Contr) animals. The serum antibody titers following influenza and diphtheria-tetanus vaccination were detected by the ELISA test. Immunohistochemical staining revealed a great number of viral antigen-positive and CD11b-positive brain cells in Flu rats compared to a very small number of the respective cells in DiTe animals and no staining in the Contr group. DiTe- and Flu-rats showed significant increases in the serum anti-tetanus toxoid and anti-influenza virus antibody levels compared to those in the Contr group. The results obtained attract attention towards the dynamic role of the CP in the immunosurveillance of the CNS. Based on the viral antigen deposits accumulated in the CP, it has been proposed that the latter can play an active role in modulation of the immune response after influenza vaccine immunization.",
publisher = "Springer, New York",
journal = "Neurophysiology",
title = "Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus",
pages = "282-276",
number = "4",
volume = "49",
doi = "10.1007/s11062-017-9682-2"
}

Maslovarić, I., Stojković, A., Kosanović, D., Marković, D., Ilić, V.,& Jovanova-Nešić, K.. (2017). Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus. in Neurophysiology
Springer, New York., 49(4), 276-282.
https://doi.org/10.1007/s11062-017-9682-2

Maslovarić I, Stojković A, Kosanović D, Marković D, Ilić V, Jovanova-Nešić K. Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus. in Neurophysiology. 2017;49(4):276-282.
doi:10.1007/s11062-017-9682-2 .

Maslovarić, Irina, Stojković, A., Kosanović, Dejana, Marković, Dragana, Ilić, Vesna, Jovanova-Nešić, Katica, "Postvaccination Accumulation of the Influenza Virus Antigen in the Rat Choroid Plexus" in Neurophysiology, 49, no. 4 (2017):276-282,
https://doi.org/10.1007/s11062-017-9682-2 . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Obrenović, Sonja
AU  - Grujić-Milanović, Jelica
AU  - Arsić, Aleksandra
AU  - Milanović, Zorana
AU  - Savić, Olivera
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/770
AB  - Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers
EP  - 227
IS  - 2
SP  - 207
VL  - 67
DO  - 10.1515/acve-2017-0018
ER  - 

@article{
author = "Kovačić, Marijana and Marković, Dragana and Maslovarić, Irina and Obrenović, Sonja and Grujić-Milanović, Jelica and Arsić, Aleksandra and Milanović, Zorana and Savić, Olivera and Fratrić, Natalija and Ilić, Vesna",
year = "2017",
abstract = "Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers",
pages = "227-207",
number = "2",
volume = "67",
doi = "10.1515/acve-2017-0018"
}

Kovačić, M., Marković, D., Maslovarić, I., Obrenović, S., Grujić-Milanović, J., Arsić, A., Milanović, Z., Savić, O., Fratrić, N.,& Ilić, V.. (2017). Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 207-227.
https://doi.org/10.1515/acve-2017-0018

Kovačić M, Marković D, Maslovarić I, Obrenović S, Grujić-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd. 2017;67(2):207-227.
doi:10.1515/acve-2017-0018 .

Kovačić, Marijana, Marković, Dragana, Maslovarić, Irina, Obrenović, Sonja, Grujić-Milanović, Jelica, Arsić, Aleksandra, Milanović, Zorana, Savić, Olivera, Fratrić, Natalija, Ilić, Vesna, "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers" in Acta Veterinaria-Beograd, 67, no. 2 (2017):207-227,
https://doi.org/10.1515/acve-2017-0018 . .

TY  - CONF
AU  - Sefer, Dijana
AU  - Miljić, Predrag
AU  - Kraguljac-Kurtović, Nada
AU  - Bizic-Radulović, S.
AU  - Čokić, Vladan
AU  - Bogdanović, Andrija
AU  - Marković, Dragana
AU  - Knežević, V.
AU  - Leković, Danijela
AU  - Gotić, Mirjana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/811
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms
EP  - 546
SP  - 545
VL  - 102
UR  - https://hdl.handle.net/21.15107/rcub_rimi_811
ER  - 

@conference{
author = "Sefer, Dijana and Miljić, Predrag and Kraguljac-Kurtović, Nada and Bizic-Radulović, S. and Čokić, Vladan and Bogdanović, Andrija and Marković, Dragana and Knežević, V. and Leković, Danijela and Gotić, Mirjana",
year = "2017",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms",
pages = "546-545",
volume = "102",
url = "https://hdl.handle.net/21.15107/rcub_rimi_811"
}

Sefer, D., Miljić, P., Kraguljac-Kurtović, N., Bizic-Radulović, S., Čokić, V., Bogdanović, A., Marković, D., Knežević, V., Leković, D.,& Gotić, M.. (2017). Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 102, 545-546.
https://hdl.handle.net/21.15107/rcub_rimi_811

Sefer D, Miljić P, Kraguljac-Kurtović N, Bizic-Radulović S, Čokić V, Bogdanović A, Marković D, Knežević V, Leković D, Gotić M. Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms. in Haematologica. 2017;102:545-546.
https://hdl.handle.net/21.15107/rcub_rimi_811 .

Sefer, Dijana, Miljić, Predrag, Kraguljac-Kurtović, Nada, Bizic-Radulović, S., Čokić, Vladan, Bogdanović, Andrija, Marković, Dragana, Knežević, V., Leković, Danijela, Gotić, Mirjana, "Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms" in Haematologica, 102 (2017):545-546,
https://hdl.handle.net/21.15107/rcub_rimi_811 .

TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Gotić, Mirjana
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
AU  - Stošić-Grujičić, Stanislava
AU  - Ivanov, Milan
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/745
AB  - Macrophage migration inhibitory factor is a well-known proinflammatory cytokine that is released during systemic stress response. Although MIF can affect erythrocyte production, the role of this cytokine in stress-induced erythropoiesis is completely unknown. To extend our previous findings showing that chronic psychological stress stimulates extramedullary erythropoiesis, here we examined whether MIF is involved in the control of stress-induced erythropoietic response. Adult male C57BL/6 wild-type (WT) and MIF-KO (knock-out) mice were subjected to 2-h daily restraint stress for either 7 or 14 consecutive days. The number of erythroid progenitors and CD71/Ter119 profile of erythroid precursors were analyzed in the bone marrow and spleen. Additionally, MIF protein expression was assessed in WT mice. Our results demonstrated that chronic restraint stress enhanced the number of both erythroid progenitors and precursors in the spleen. Stress-induced increase in the number of splenic late erythroid progenitors as well as in the percentage of CD71(+)Ter119(+)-double-positive precursors was significantly more pronounced in MIF-KO mice compared to WT animals. Furthermore, repeatedly stressed WT animals demonstrated an augmented MIF expression in the spleen. Unlike the spleen, the bone marrow of chronically stressed WT mice exhibited less prominent changes in erythropoietic stress response and no significant alteration in MIF expression. In addition, MIF deficiency did not influence the bone marrow erythropoiesis in stressed animals. These findings suggest that MIF regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions.
PB  - Springer, New York
T2  - Histochemistry & Cell Biology
T1  - Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis
EP  - 324
IS  - 3
SP  - 311
VL  - 146
DO  - 10.1007/s00418-016-1442-7
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Budeč, Mirela and Marković, Dragana and Gotić, Mirjana and Mitrović-Ajtić, Olivera and Mojsilović, Slavko and Stošić-Grujičić, Stanislava and Ivanov, Milan and Jovčić, Gordana and Čokić, Vladan",
year = "2016",
abstract = "Macrophage migration inhibitory factor is a well-known proinflammatory cytokine that is released during systemic stress response. Although MIF can affect erythrocyte production, the role of this cytokine in stress-induced erythropoiesis is completely unknown. To extend our previous findings showing that chronic psychological stress stimulates extramedullary erythropoiesis, here we examined whether MIF is involved in the control of stress-induced erythropoietic response. Adult male C57BL/6 wild-type (WT) and MIF-KO (knock-out) mice were subjected to 2-h daily restraint stress for either 7 or 14 consecutive days. The number of erythroid progenitors and CD71/Ter119 profile of erythroid precursors were analyzed in the bone marrow and spleen. Additionally, MIF protein expression was assessed in WT mice. Our results demonstrated that chronic restraint stress enhanced the number of both erythroid progenitors and precursors in the spleen. Stress-induced increase in the number of splenic late erythroid progenitors as well as in the percentage of CD71(+)Ter119(+)-double-positive precursors was significantly more pronounced in MIF-KO mice compared to WT animals. Furthermore, repeatedly stressed WT animals demonstrated an augmented MIF expression in the spleen. Unlike the spleen, the bone marrow of chronically stressed WT mice exhibited less prominent changes in erythropoietic stress response and no significant alteration in MIF expression. In addition, MIF deficiency did not influence the bone marrow erythropoiesis in stressed animals. These findings suggest that MIF regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions.",
publisher = "Springer, New York",
journal = "Histochemistry & Cell Biology",
title = "Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis",
pages = "324-311",
number = "3",
volume = "146",
doi = "10.1007/s00418-016-1442-7"
}

Vignjević-Petrinović, S., Budeč, M., Marković, D., Gotić, M., Mitrović-Ajtić, O., Mojsilović, S., Stošić-Grujičić, S., Ivanov, M., Jovčić, G.,& Čokić, V.. (2016). Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. in Histochemistry & Cell Biology
Springer, New York., 146(3), 311-324.
https://doi.org/10.1007/s00418-016-1442-7

Vignjević-Petrinović S, Budeč M, Marković D, Gotić M, Mitrović-Ajtić O, Mojsilović S, Stošić-Grujičić S, Ivanov M, Jovčić G, Čokić V. Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. in Histochemistry & Cell Biology. 2016;146(3):311-324.
doi:10.1007/s00418-016-1442-7 .

Vignjević-Petrinović, Sanja, Budeč, Mirela, Marković, Dragana, Gotić, Mirjana, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, Stošić-Grujičić, Stanislava, Ivanov, Milan, Jovčić, Gordana, Čokić, Vladan, "Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis" in Histochemistry & Cell Biology, 146, no. 3 (2016):311-324,
https://doi.org/10.1007/s00418-016-1442-7 . .

TY  - CONF
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Sefer, Dijana
AU  - Beleslin-Čokić, Bojana
AU  - Milić, Nataša
AU  - Mitrović-Ajtić, Olivera
AU  - Subotički, Tijana
AU  - Marković, Dragana
AU  - Buac, M.
AU  - Diklić, Miloš
AU  - Colović, N.
AU  - Bodrozić, J.
AU  - Čokić, Vladan
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/749
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Bone marrow microvessel density and plasma angiogenic factors in BCR-ABL1 negative myeloproliferative neoplasms: a study of 90 patients with clinicopathological and molecular correlations
EP  - 268
SP  - 267
VL  - 101
UR  - https://hdl.handle.net/21.15107/rcub_rimi_749
ER  - 

@conference{
author = "Leković, Danijela and Gotić, Mirjana and Sefer, Dijana and Beleslin-Čokić, Bojana and Milić, Nataša and Mitrović-Ajtić, Olivera and Subotički, Tijana and Marković, Dragana and Buac, M. and Diklić, Miloš and Colović, N. and Bodrozić, J. and Čokić, Vladan",
year = "2016",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Bone marrow microvessel density and plasma angiogenic factors in BCR-ABL1 negative myeloproliferative neoplasms: a study of 90 patients with clinicopathological and molecular correlations",
pages = "268-267",
volume = "101",
url = "https://hdl.handle.net/21.15107/rcub_rimi_749"
}

Leković, D., Gotić, M., Sefer, D., Beleslin-Čokić, B., Milić, N., Mitrović-Ajtić, O., Subotički, T., Marković, D., Buac, M., Diklić, M., Colović, N., Bodrozić, J.,& Čokić, V.. (2016). Bone marrow microvessel density and plasma angiogenic factors in BCR-ABL1 negative myeloproliferative neoplasms: a study of 90 patients with clinicopathological and molecular correlations. in Haematologica
Ferrata Storti Foundation, Pavia., 101, 267-268.
https://hdl.handle.net/21.15107/rcub_rimi_749

Leković D, Gotić M, Sefer D, Beleslin-Čokić B, Milić N, Mitrović-Ajtić O, Subotički T, Marković D, Buac M, Diklić M, Colović N, Bodrozić J, Čokić V. Bone marrow microvessel density and plasma angiogenic factors in BCR-ABL1 negative myeloproliferative neoplasms: a study of 90 patients with clinicopathological and molecular correlations. in Haematologica. 2016;101:267-268.
https://hdl.handle.net/21.15107/rcub_rimi_749 .

Leković, Danijela, Gotić, Mirjana, Sefer, Dijana, Beleslin-Čokić, Bojana, Milić, Nataša, Mitrović-Ajtić, Olivera, Subotički, Tijana, Marković, Dragana, Buac, M., Diklić, Miloš, Colović, N., Bodrozić, J., Čokić, Vladan, "Bone marrow microvessel density and plasma angiogenic factors in BCR-ABL1 negative myeloproliferative neoplasms: a study of 90 patients with clinicopathological and molecular correlations" in Haematologica, 101 (2016):267-268,
https://hdl.handle.net/21.15107/rcub_rimi_749 .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/717
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
EP  - 142
SP  - 135
VL  - 106
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 

@article{
author = "Kovačić, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
pages = "142-135",
volume = "106",
doi = "10.1016/j.rvsc.2016.04.002"
}

Kovačić, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002

Kovačić M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .

Kovačić, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .