TY  - JOUR
AU  - Momčilović, Sanja
AU  - Bogdanović, Andrija
AU  - Milošević, Maja
AU  - Mojsilović, Slavko
AU  - Marković, Dragana
AU  - Kočović, Dušica M.
AU  - Vignjević-Petrinović, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1329
AB  - Psychological stress is a significant contributor to various chronic diseases and affects multiple physiological processes including erythropoiesis. This study aimed to examine the tissue-specific contributions of macrophages and extracellular ATP, as a signal of disturbed tissue homeostasis, to erythropoiesis under conditions of repeated psychological stress. Adult male BALB/c mice were subjected to 2 h daily restraint stress for seven consecutive days. Clodronate-liposomes were used to deplete resident macrophages from the bone marrow and spleen two days prior to the first restraint procedure, as well as newly recruited macrophages, every third day for the duration of the experiment. Repeated stress induced a considerable increase in the number of erythroid progenitor cells as well as in the percentage of CD71+/Ter119+ and CD71−/Ter119+ cells in the bone marrow and spleen. Macrophage depletion completely abolished the stimulative effect of repeated stress on immature erythroid cells, and prevented stress-induced increases in ATP levels, P2X7 receptor (P2X7R) expression, and ectonucleotidase CD39 activity and expression in the bone marrow and spleen. The obtained results demonstrate the stimulative effects of repeated stress on erythroid cells, extracellular ATP levels, P2X7R expression, CD39 activity and expression within the bone marrow and spleen, as well as the essential role of macrophages in stress-induced changes.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress
IS  - 14
SP  - 11373
VL  - 24
DO  - 10.3390/ijms241411373
ER  - 

@article{
author = "Momčilović, Sanja and Bogdanović, Andrija and Milošević, Maja and Mojsilović, Slavko and Marković, Dragana and Kočović, Dušica M. and Vignjević-Petrinović, Sanja",
year = "2023",
abstract = "Psychological stress is a significant contributor to various chronic diseases and affects multiple physiological processes including erythropoiesis. This study aimed to examine the tissue-specific contributions of macrophages and extracellular ATP, as a signal of disturbed tissue homeostasis, to erythropoiesis under conditions of repeated psychological stress. Adult male BALB/c mice were subjected to 2 h daily restraint stress for seven consecutive days. Clodronate-liposomes were used to deplete resident macrophages from the bone marrow and spleen two days prior to the first restraint procedure, as well as newly recruited macrophages, every third day for the duration of the experiment. Repeated stress induced a considerable increase in the number of erythroid progenitor cells as well as in the percentage of CD71+/Ter119+ and CD71−/Ter119+ cells in the bone marrow and spleen. Macrophage depletion completely abolished the stimulative effect of repeated stress on immature erythroid cells, and prevented stress-induced increases in ATP levels, P2X7 receptor (P2X7R) expression, and ectonucleotidase CD39 activity and expression in the bone marrow and spleen. The obtained results demonstrate the stimulative effects of repeated stress on erythroid cells, extracellular ATP levels, P2X7R expression, CD39 activity and expression within the bone marrow and spleen, as well as the essential role of macrophages in stress-induced changes.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress",
number = "14",
pages = "11373",
volume = "24",
doi = "10.3390/ijms241411373"
}

Momčilović, S., Bogdanović, A., Milošević, M., Mojsilović, S., Marković, D., Kočović, D. M.,& Vignjević-Petrinović, S.. (2023). Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 24(14), 11373.
https://doi.org/10.3390/ijms241411373

Momčilović S, Bogdanović A, Milošević M, Mojsilović S, Marković D, Kočović DM, Vignjević-Petrinović S. Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress. in International Journal of Molecular Sciences. 2023;24(14):11373.
doi:10.3390/ijms241411373 .

Momčilović, Sanja, Bogdanović, Andrija, Milošević, Maja, Mojsilović, Slavko, Marković, Dragana, Kočović, Dušica M., Vignjević-Petrinović, Sanja, "Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress" in International Journal of Molecular Sciences, 24, no. 14 (2023):11373,
https://doi.org/10.3390/ijms241411373 . .

TY  - JOUR
AU  - Leković, Danijela
AU  - Bogdanović, Andrija
AU  - Sobas, Marta
AU  - Arsenović, Isidora
AU  - Smiljanić, Mihailo
AU  - Ivanović, Jelena
AU  - Bodrožić, Jelena
AU  - Čokić, Vladan
AU  - Milić, Nataša
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1344
AB  - Essential thrombocythemia (ET) and prefibrotic primary myelofibrosis (prePMF) initially have a similar phenotypic presentation with thrombocytosis. The aim of our study was to determine significant clinical-laboratory parameters at presentation to differentiate prePMF from ET as well as to develop and validate a predictive diagnostic prePMF model. This retrospective study included 464 patients divided into ET (289 pts) and prePMF (175 pts) groups. The model was built using data from a development cohort (229 pts; 143 ET, 86 prePMF), which was then tested in an internal validation cohort (235 pts; 146 ET, 89 prePMF). The most important prePMF predictors in the multivariate logistic model were age ≥ 60 years (RR = 2.2), splenomegaly (RR = 13.2), and increased lactat-dehidrogenase (RR = 2.8). Risk scores were assigned according to derived relative risk (RR) for age ≥ 60 years (1 point), splenomegaly (2 points), and increased lactat-dehidrogenase (1 point). Positive predictive value (PPV) for pre-PMF diagnosis with a score of ≥points was 69.8%, while for a score of ≥3 it was 88.2%. Diagnostic performance had similar values in the validation cohort. In MPN patients with thrombocytosis at presentation, the application of the new model enables differentiation of pre-PMF from ET, which is clinically relevant considering that these diseases have different prognoses and treatments.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Cancers
T2  - Cancers
T1  - Easily Applicable Predictive Score for Differential Diagnosis of Prefibrotic Primary Myelofibrosis from Essential Thrombocythemia
IS  - 16
SP  - 4180
VL  - 15
DO  - 10.3390/cancers15164180
ER  - 

@article{
author = "Leković, Danijela and Bogdanović, Andrija and Sobas, Marta and Arsenović, Isidora and Smiljanić, Mihailo and Ivanović, Jelena and Bodrožić, Jelena and Čokić, Vladan and Milić, Nataša",
year = "2023",
abstract = "Essential thrombocythemia (ET) and prefibrotic primary myelofibrosis (prePMF) initially have a similar phenotypic presentation with thrombocytosis. The aim of our study was to determine significant clinical-laboratory parameters at presentation to differentiate prePMF from ET as well as to develop and validate a predictive diagnostic prePMF model. This retrospective study included 464 patients divided into ET (289 pts) and prePMF (175 pts) groups. The model was built using data from a development cohort (229 pts; 143 ET, 86 prePMF), which was then tested in an internal validation cohort (235 pts; 146 ET, 89 prePMF). The most important prePMF predictors in the multivariate logistic model were age ≥ 60 years (RR = 2.2), splenomegaly (RR = 13.2), and increased lactat-dehidrogenase (RR = 2.8). Risk scores were assigned according to derived relative risk (RR) for age ≥ 60 years (1 point), splenomegaly (2 points), and increased lactat-dehidrogenase (1 point). Positive predictive value (PPV) for pre-PMF diagnosis with a score of ≥points was 69.8%, while for a score of ≥3 it was 88.2%. Diagnostic performance had similar values in the validation cohort. In MPN patients with thrombocytosis at presentation, the application of the new model enables differentiation of pre-PMF from ET, which is clinically relevant considering that these diseases have different prognoses and treatments.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Cancers, Cancers",
title = "Easily Applicable Predictive Score for Differential Diagnosis of Prefibrotic Primary Myelofibrosis from Essential Thrombocythemia",
number = "16",
pages = "4180",
volume = "15",
doi = "10.3390/cancers15164180"
}

Leković, D., Bogdanović, A., Sobas, M., Arsenović, I., Smiljanić, M., Ivanović, J., Bodrožić, J., Čokić, V.,& Milić, N.. (2023). Easily Applicable Predictive Score for Differential Diagnosis of Prefibrotic Primary Myelofibrosis from Essential Thrombocythemia. in Cancers
Multidisciplinary Digital Publishing Institute (MDPI)., 15(16), 4180.
https://doi.org/10.3390/cancers15164180

Leković D, Bogdanović A, Sobas M, Arsenović I, Smiljanić M, Ivanović J, Bodrožić J, Čokić V, Milić N. Easily Applicable Predictive Score for Differential Diagnosis of Prefibrotic Primary Myelofibrosis from Essential Thrombocythemia. in Cancers. 2023;15(16):4180.
doi:10.3390/cancers15164180 .

Leković, Danijela, Bogdanović, Andrija, Sobas, Marta, Arsenović, Isidora, Smiljanić, Mihailo, Ivanović, Jelena, Bodrožić, Jelena, Čokić, Vladan, Milić, Nataša, "Easily Applicable Predictive Score for Differential Diagnosis of Prefibrotic Primary Myelofibrosis from Essential Thrombocythemia" in Cancers, 15, no. 16 (2023):4180,
https://doi.org/10.3390/cancers15164180 . .

TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Bogdanović, Andrija
AU  - Marković, Dragana
AU  - Mitrović-Ajtić, Olivera
AU  - Subotički, Tijana
AU  - Diklić, Miloš
AU  - Vukotić, Milica
AU  - Dragojević, Teodora
AU  - Živković, Emilija
AU  - Santibanez, Juan F.
AU  - Čokić, Vladan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1202
AB  - Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the pro-liferation-related AKT/mTOR signaling pathway and the relationship with inflammatory factors in chronic myelogenous leukemia (CML). The activity of the antioxidant enzymes superoxide dis-mutase, glutathione peroxidase, and catalase is reduced in erythrocytes while levels of the oxidative stress markers malondialdehyde and protein carbonyl are elevated in the plasma of patients with CML. In addition, nitrogen species (nitrotyrosine, iNOS, eNOS) and inflammation markers (IL-6, NFkB, and S100 protein) were increased in granulocytes of CML while anti-inflammatory levels of IL-10 were decreased in plasma. CML granulocytes exhibited greater resistance to cytotoxic H2O2 activity compared to healthy subjects. Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H2O2) in granulocytes and erythroleukemic K562 cells. IL-6 caused oxidative stress and DNA damage that was mitigated using antioxidant or inhibition of inflammatory NFkB transcription factor in K562 cells. We demonstrated the presence of oxidative and ni-trosative stress in CML, with the former mediated by AKT/mTOR signaling and stimulated by in-flammation.
PB  - MDPI
T2  - Biomolecules
T1  - Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
IS  - 2
SP  - 247
VL  - 12
DO  - 10.3390/biom12020247
ER  - 

@article{
author = "Đikić, Dragoslava and Bogdanović, Andrija and Marković, Dragana and Mitrović-Ajtić, Olivera and Subotički, Tijana and Diklić, Miloš and Vukotić, Milica and Dragojević, Teodora and Živković, Emilija and Santibanez, Juan F. and Čokić, Vladan",
year = "2022",
abstract = "Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the pro-liferation-related AKT/mTOR signaling pathway and the relationship with inflammatory factors in chronic myelogenous leukemia (CML). The activity of the antioxidant enzymes superoxide dis-mutase, glutathione peroxidase, and catalase is reduced in erythrocytes while levels of the oxidative stress markers malondialdehyde and protein carbonyl are elevated in the plasma of patients with CML. In addition, nitrogen species (nitrotyrosine, iNOS, eNOS) and inflammation markers (IL-6, NFkB, and S100 protein) were increased in granulocytes of CML while anti-inflammatory levels of IL-10 were decreased in plasma. CML granulocytes exhibited greater resistance to cytotoxic H2O2 activity compared to healthy subjects. Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H2O2) in granulocytes and erythroleukemic K562 cells. IL-6 caused oxidative stress and DNA damage that was mitigated using antioxidant or inhibition of inflammatory NFkB transcription factor in K562 cells. We demonstrated the presence of oxidative and ni-trosative stress in CML, with the former mediated by AKT/mTOR signaling and stimulated by in-flammation.",
publisher = "MDPI",
journal = "Biomolecules",
title = "Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia",
number = "2",
pages = "247",
volume = "12",
doi = "10.3390/biom12020247"
}

Đikić, D., Bogdanović, A., Marković, D., Mitrović-Ajtić, O., Subotički, T., Diklić, M., Vukotić, M., Dragojević, T., Živković, E., Santibanez, J. F.,& Čokić, V.. (2022). Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia. in Biomolecules
MDPI., 12(2), 247.
https://doi.org/10.3390/biom12020247

Đikić D, Bogdanović A, Marković D, Mitrović-Ajtić O, Subotički T, Diklić M, Vukotić M, Dragojević T, Živković E, Santibanez JF, Čokić V. Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia. in Biomolecules. 2022;12(2):247.
doi:10.3390/biom12020247 .

Đikić, Dragoslava, Bogdanović, Andrija, Marković, Dragana, Mitrović-Ajtić, Olivera, Subotički, Tijana, Diklić, Miloš, Vukotić, Milica, Dragojević, Teodora, Živković, Emilija, Santibanez, Juan F., Čokić, Vladan, "Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia" in Biomolecules, 12, no. 2 (2022):247,
https://doi.org/10.3390/biom12020247 . .

TY  - JOUR
AU  - Šefer, Dijana
AU  - Miljić, Predrag
AU  - Kraguljac-Kurtović, Nada
AU  - Bižić-Radulović, Sandra
AU  - Bogdanović, Andrija
AU  - Knežević, Vesna
AU  - Marković, Dragana
AU  - Beleslin-Čokić, Bojana
AU  - Novaković, Ivana
AU  - Marinković, Jelena
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Čokić, Vladan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1231
AB  - Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA.
T2  - International Journal of Laboratory Hematology
T1  - Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms
EP  - 312
IS  - 2
SP  - 302
VL  - 44
DO  - 10.1111/ijlh.13754
ER  - 

@article{
author = "Šefer, Dijana and Miljić, Predrag and Kraguljac-Kurtović, Nada and Bižić-Radulović, Sandra and Bogdanović, Andrija and Knežević, Vesna and Marković, Dragana and Beleslin-Čokić, Bojana and Novaković, Ivana and Marinković, Jelena and Leković, Danijela and Gotić, Mirjana and Čokić, Vladan",
year = "2022",
abstract = "Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA.",
journal = "International Journal of Laboratory Hematology",
title = "Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms",
pages = "312-302",
number = "2",
volume = "44",
doi = "10.1111/ijlh.13754"
}

Šefer, D., Miljić, P., Kraguljac-Kurtović, N., Bižić-Radulović, S., Bogdanović, A., Knežević, V., Marković, D., Beleslin-Čokić, B., Novaković, I., Marinković, J., Leković, D., Gotić, M.,& Čokić, V.. (2022). Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms. in International Journal of Laboratory Hematology, 44(2), 302-312.
https://doi.org/10.1111/ijlh.13754

Šefer D, Miljić P, Kraguljac-Kurtović N, Bižić-Radulović S, Bogdanović A, Knežević V, Marković D, Beleslin-Čokić B, Novaković I, Marinković J, Leković D, Gotić M, Čokić V. Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms. in International Journal of Laboratory Hematology. 2022;44(2):302-312.
doi:10.1111/ijlh.13754 .

Šefer, Dijana, Miljić, Predrag, Kraguljac-Kurtović, Nada, Bižić-Radulović, Sandra, Bogdanović, Andrija, Knežević, Vesna, Marković, Dragana, Beleslin-Čokić, Bojana, Novaković, Ivana, Marinković, Jelena, Leković, Danijela, Gotić, Mirjana, Čokić, Vladan, "Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms" in International Journal of Laboratory Hematology, 44, no. 2 (2022):302-312,
https://doi.org/10.1111/ijlh.13754 . .

TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Marković, Dragana
AU  - Bogdanović, Andrija
AU  - Mitrović-Ajtić, Olivera
AU  - Subotički, Tijana
AU  - Diklić, Miloš
AU  - Beleslin-Čokić, Bojana
AU  - Bjelica, Sunčica
AU  - Kovačić, Marijana
AU  - Čokić, Vladan
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/836
AB  - Purpose: A common feature of malignancies is increased reactive oxygen species (ROS) and reactive nitrogen species (RNS). We analyzed the influence of oxidative and nitrosative stress on the activation of AKT/mTOR signaling pathway in myeloproliferative neoplasms (MPN). Methods: Oxidative stress-induced gene expression in circulatory CD34(+) cells of MPN patients was studied by microarray analysis. Biomarkers of oxidative and nitrosative stress were determined using spectrophotometry in plasma and erythrocyte lysate. The levels of nitrotyrosine, inducible NO synthase (iNOS) and AKT/mTOR/p70S6K phosphorylation were determined by immunocytochemistry and immunoblotting in granulocytes of MPN patients. Results: Antioxidants superoxide dismutase 2 (SOD2) and glutathione peroxidase 1 (GPx1) gene expression were increased in circulatory CD34(+) cells, while SODI and GPx enzymes were reduced in the erythrocytes of MPN. Plasma malonyl-dialdehyde and protein carbonyl levels were elevated in MPN. The total antioxidant capacity in plasma and erythrocyte catalase (CT) activities was the most prominent in primary myelofibrosis (PMF) with JAK2V617F heterozygosity. The total nitrite/ nitrate (NOx) level was augmented in the plasma of PMF patients (p  lt 0.001), while nitrotyrosine and iNOS were generally increased in the granulocytes of MPN patients. Activation of AKT/m TOR signaling was the most significant in PMF (p lt 0.01), but demonstrated JAK2V617F dependence and consequent p70S6K phosphorylation in the granulocytes of essential thrombocytemia (ET) and polycythemia vera (PV) patients. Hydrogen peroxide stimulated mTOR pathway, iNOS and nitrotyrosine quantities, the last one prevented by the antioxidant nacetyl-cysteine (NAC) in the granulocytes of MPN. Conclusion: Our study showed increased levels of oxidative and nitrosative stress parameters in MPN with JAK2V617F dependence. The ROS enhanced the constitutive activation of AKT/mTOR signaling and nitrosative parameters in MPN.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway
EP  - 1491
IS  - 5
SP  - 1481
VL  - 23
UR  - https://hdl.handle.net/21.15107/rcub_rimi_836
ER  - 

@article{
author = "Đikić, Dragoslava and Marković, Dragana and Bogdanović, Andrija and Mitrović-Ajtić, Olivera and Subotički, Tijana and Diklić, Miloš and Beleslin-Čokić, Bojana and Bjelica, Sunčica and Kovačić, Marijana and Čokić, Vladan",
year = "2018",
abstract = "Purpose: A common feature of malignancies is increased reactive oxygen species (ROS) and reactive nitrogen species (RNS). We analyzed the influence of oxidative and nitrosative stress on the activation of AKT/mTOR signaling pathway in myeloproliferative neoplasms (MPN). Methods: Oxidative stress-induced gene expression in circulatory CD34(+) cells of MPN patients was studied by microarray analysis. Biomarkers of oxidative and nitrosative stress were determined using spectrophotometry in plasma and erythrocyte lysate. The levels of nitrotyrosine, inducible NO synthase (iNOS) and AKT/mTOR/p70S6K phosphorylation were determined by immunocytochemistry and immunoblotting in granulocytes of MPN patients. Results: Antioxidants superoxide dismutase 2 (SOD2) and glutathione peroxidase 1 (GPx1) gene expression were increased in circulatory CD34(+) cells, while SODI and GPx enzymes were reduced in the erythrocytes of MPN. Plasma malonyl-dialdehyde and protein carbonyl levels were elevated in MPN. The total antioxidant capacity in plasma and erythrocyte catalase (CT) activities was the most prominent in primary myelofibrosis (PMF) with JAK2V617F heterozygosity. The total nitrite/ nitrate (NOx) level was augmented in the plasma of PMF patients (p  lt 0.001), while nitrotyrosine and iNOS were generally increased in the granulocytes of MPN patients. Activation of AKT/m TOR signaling was the most significant in PMF (p lt 0.01), but demonstrated JAK2V617F dependence and consequent p70S6K phosphorylation in the granulocytes of essential thrombocytemia (ET) and polycythemia vera (PV) patients. Hydrogen peroxide stimulated mTOR pathway, iNOS and nitrotyrosine quantities, the last one prevented by the antioxidant nacetyl-cysteine (NAC) in the granulocytes of MPN. Conclusion: Our study showed increased levels of oxidative and nitrosative stress parameters in MPN with JAK2V617F dependence. The ROS enhanced the constitutive activation of AKT/mTOR signaling and nitrosative parameters in MPN.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway",
pages = "1491-1481",
number = "5",
volume = "23",
url = "https://hdl.handle.net/21.15107/rcub_rimi_836"
}

Đikić, D., Marković, D., Bogdanović, A., Mitrović-Ajtić, O., Subotički, T., Diklić, M., Beleslin-Čokić, B., Bjelica, S., Kovačić, M.,& Čokić, V.. (2018). Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 23(5), 1481-1491.
https://hdl.handle.net/21.15107/rcub_rimi_836

Đikić D, Marković D, Bogdanović A, Mitrović-Ajtić O, Subotički T, Diklić M, Beleslin-Čokić B, Bjelica S, Kovačić M, Čokić V. Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway. in Journal of BUON. 2018;23(5):1481-1491.
https://hdl.handle.net/21.15107/rcub_rimi_836 .

Đikić, Dragoslava, Marković, Dragana, Bogdanović, Andrija, Mitrović-Ajtić, Olivera, Subotički, Tijana, Diklić, Miloš, Beleslin-Čokić, Bojana, Bjelica, Sunčica, Kovačić, Marijana, Čokić, Vladan, "Oxidative and nitrosative stress in myeloproliferative neoplasms: the impact on the AKT/mTOR signaling pathway" in Journal of BUON, 23, no. 5 (2018):1481-1491,
https://hdl.handle.net/21.15107/rcub_rimi_836 .

TY  - JOUR
AU  - Šefer, Dijana
AU  - Bižić-Radulović, Sandra
AU  - Kraguljac-Kurtović, Nada
AU  - Bogdanović, Andrija
AU  - Čokić, Vladan
AU  - Miljić, Predrag
AU  - Beleslin-Čokić, Bojana
AU  - Knežević, Vesna
AU  - Mitrović-Ajtić, Olivera
AU  - Leković, Danijela
AU  - Gotić, Mirjana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/795
AB  - Introduction: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are characterized by overproduction of one or more blood cell lines. Methods: We studied the proliferative characteristics of 91 patients with de novo Ph-MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34(+) cells was determined by flow cytometry. Results: The total number of PB CFC in primary myelofibrosis (PMF) was increased compared to the control sample (P  lt  0.01) and essential thrombocythemia (ET) (P  lt  0.05). The highest number of BM and PB EC was observed in polycythemia vera (PV) (P  lt  0.01). Increased levels of CD34(+) cells characterized early-prefibrotic (57%) and advanced-fibrotic PMF (90%) as compared to PV (34%) and ET (32%) (P  lt  0.01). In the whole Ph-MPN group, the total number of PB CFC (P  lt  0.01), PB EC (P  lt  0.05), and CD34+ cells (P  lt  0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD34(+) cells in PMF correlated with the total number of PB EC (P  lt  0.05) and degree of BM fibrosis (P  lt  0.01). Conclusions: Exploration of the PB proliferative characteristics of Ph-MPN on diagnosis may be helpful in revealing early-prefibrotic PMF. Monitoring the levels of circulating CD34(+) cells may provide a sensitive indicator of fibrotic evolution in PV and PMF.
PB  - Wiley, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications
EP  - 31
IS  - 1
SP  - 21
VL  - 39
DO  - 10.1111/ijlh.12564
ER  - 

@article{
author = "Šefer, Dijana and Bižić-Radulović, Sandra and Kraguljac-Kurtović, Nada and Bogdanović, Andrija and Čokić, Vladan and Miljić, Predrag and Beleslin-Čokić, Bojana and Knežević, Vesna and Mitrović-Ajtić, Olivera and Leković, Danijela and Gotić, Mirjana",
year = "2017",
abstract = "Introduction: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are characterized by overproduction of one or more blood cell lines. Methods: We studied the proliferative characteristics of 91 patients with de novo Ph-MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34(+) cells was determined by flow cytometry. Results: The total number of PB CFC in primary myelofibrosis (PMF) was increased compared to the control sample (P  lt  0.01) and essential thrombocythemia (ET) (P  lt  0.05). The highest number of BM and PB EC was observed in polycythemia vera (PV) (P  lt  0.01). Increased levels of CD34(+) cells characterized early-prefibrotic (57%) and advanced-fibrotic PMF (90%) as compared to PV (34%) and ET (32%) (P  lt  0.01). In the whole Ph-MPN group, the total number of PB CFC (P  lt  0.01), PB EC (P  lt  0.05), and CD34+ cells (P  lt  0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD34(+) cells in PMF correlated with the total number of PB EC (P  lt  0.05) and degree of BM fibrosis (P  lt  0.01). Conclusions: Exploration of the PB proliferative characteristics of Ph-MPN on diagnosis may be helpful in revealing early-prefibrotic PMF. Monitoring the levels of circulating CD34(+) cells may provide a sensitive indicator of fibrotic evolution in PV and PMF.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications",
pages = "31-21",
number = "1",
volume = "39",
doi = "10.1111/ijlh.12564"
}

Šefer, D., Bižić-Radulović, S., Kraguljac-Kurtović, N., Bogdanović, A., Čokić, V., Miljić, P., Beleslin-Čokić, B., Knežević, V., Mitrović-Ajtić, O., Leković, D.,& Gotić, M.. (2017). Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications. in International Journal of Laboratory Hematology
Wiley, Hoboken., 39(1), 21-31.
https://doi.org/10.1111/ijlh.12564

Šefer D, Bižić-Radulović S, Kraguljac-Kurtović N, Bogdanović A, Čokić V, Miljić P, Beleslin-Čokić B, Knežević V, Mitrović-Ajtić O, Leković D, Gotić M. Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications. in International Journal of Laboratory Hematology. 2017;39(1):21-31.
doi:10.1111/ijlh.12564 .

Šefer, Dijana, Bižić-Radulović, Sandra, Kraguljac-Kurtović, Nada, Bogdanović, Andrija, Čokić, Vladan, Miljić, Predrag, Beleslin-Čokić, Bojana, Knežević, Vesna, Mitrović-Ajtić, Olivera, Leković, Danijela, Gotić, Mirjana, "Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications" in International Journal of Laboratory Hematology, 39, no. 1 (2017):21-31,
https://doi.org/10.1111/ijlh.12564 . .

TY  - CONF
AU  - Sefer, Dijana
AU  - Miljić, Predrag
AU  - Kraguljac-Kurtović, Nada
AU  - Bizic-Radulović, S.
AU  - Čokić, Vladan
AU  - Bogdanović, Andrija
AU  - Marković, Dragana
AU  - Knežević, V.
AU  - Leković, Danijela
AU  - Gotić, Mirjana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/811
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms
EP  - 546
SP  - 545
VL  - 102
UR  - https://hdl.handle.net/21.15107/rcub_rimi_811
ER  - 

@conference{
author = "Sefer, Dijana and Miljić, Predrag and Kraguljac-Kurtović, Nada and Bizic-Radulović, S. and Čokić, Vladan and Bogdanović, Andrija and Marković, Dragana and Knežević, V. and Leković, Danijela and Gotić, Mirjana",
year = "2017",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms",
pages = "546-545",
volume = "102",
url = "https://hdl.handle.net/21.15107/rcub_rimi_811"
}

Sefer, D., Miljić, P., Kraguljac-Kurtović, N., Bizic-Radulović, S., Čokić, V., Bogdanović, A., Marković, D., Knežević, V., Leković, D.,& Gotić, M.. (2017). Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 102, 545-546.
https://hdl.handle.net/21.15107/rcub_rimi_811

Sefer D, Miljić P, Kraguljac-Kurtović N, Bizic-Radulović S, Čokić V, Bogdanović A, Marković D, Knežević V, Leković D, Gotić M. Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms. in Haematologica. 2017;102:545-546.
https://hdl.handle.net/21.15107/rcub_rimi_811 .

Sefer, Dijana, Miljić, Predrag, Kraguljac-Kurtović, Nada, Bizic-Radulović, S., Čokić, Vladan, Bogdanović, Andrija, Marković, Dragana, Knežević, V., Leković, Danijela, Gotić, Mirjana, "Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms" in Haematologica, 102 (2017):545-546,
https://hdl.handle.net/21.15107/rcub_rimi_811 .

TY  - JOUR
AU  - Čokić, Vladan
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Kraguljac-Kurtović, Nada
AU  - Mojsilović, Sonja
AU  - Sefer, Dijana
AU  - Mitrović-Ajtić, Olivera
AU  - Milošević, Violeta
AU  - Bogdanović, Andrija
AU  - Đikić, Dragoslava
AU  - Milenković, Pavle B.
AU  - Puri, Raj K.
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/631
AB  - Purpose: We compared the gene expression profile of peripheral blood CD34(+) cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34(+) cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012 +/- SD of CD34(+) cells/mu l in peripheral blood. Results: The gene expression profile was more prominent in CML CD34(+) cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34(+) cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34(+) cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34(+) cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Blood Cells Molecules & Diseases
T1  - Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia
EP  - 381
IS  - 4
SP  - 373
VL  - 55
DO  - 10.1016/j.bcmd.2015.08.002
ER  - 

@article{
author = "Čokić, Vladan and Mojsilović, Slavko and Jauković, Aleksandra and Kraguljac-Kurtović, Nada and Mojsilović, Sonja and Sefer, Dijana and Mitrović-Ajtić, Olivera and Milošević, Violeta and Bogdanović, Andrija and Đikić, Dragoslava and Milenković, Pavle B. and Puri, Raj K.",
year = "2015",
abstract = "Purpose: We compared the gene expression profile of peripheral blood CD34(+) cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34(+) cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012 +/- SD of CD34(+) cells/mu l in peripheral blood. Results: The gene expression profile was more prominent in CML CD34(+) cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34(+) cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34(+) cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34(+) cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Blood Cells Molecules & Diseases",
title = "Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia",
pages = "381-373",
number = "4",
volume = "55",
doi = "10.1016/j.bcmd.2015.08.002"
}

Čokić, V., Mojsilović, S., Jauković, A., Kraguljac-Kurtović, N., Mojsilović, S., Sefer, D., Mitrović-Ajtić, O., Milošević, V., Bogdanović, A., Đikić, D., Milenković, P. B.,& Puri, R. K.. (2015). Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia. in Blood Cells Molecules & Diseases
Academic Press Inc Elsevier Science, San Diego., 55(4), 373-381.
https://doi.org/10.1016/j.bcmd.2015.08.002

Čokić V, Mojsilović S, Jauković A, Kraguljac-Kurtović N, Mojsilović S, Sefer D, Mitrović-Ajtić O, Milošević V, Bogdanović A, Đikić D, Milenković PB, Puri RK. Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia. in Blood Cells Molecules & Diseases. 2015;55(4):373-381.
doi:10.1016/j.bcmd.2015.08.002 .

Čokić, Vladan, Mojsilović, Slavko, Jauković, Aleksandra, Kraguljac-Kurtović, Nada, Mojsilović, Sonja, Sefer, Dijana, Mitrović-Ajtić, Olivera, Milošević, Violeta, Bogdanović, Andrija, Đikić, Dragoslava, Milenković, Pavle B., Puri, Raj K., "Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia" in Blood Cells Molecules & Diseases, 55, no. 4 (2015):373-381,
https://doi.org/10.1016/j.bcmd.2015.08.002 . .

TY  - JOUR
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Peruničić-Jovanović, Maja
AU  - Vidović, Ana
AU  - Bogdanović, Andrija
AU  - Janković, Gradimir
AU  - Čokić, Vladan
AU  - Milić, Nataša
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/540
AB  - The widely used current International Prognostic Scoring System (IPSS) for primary myelofibrosis (PMF) is based on clinical parameters. The objective of this study was to identify additional prognostic factors at the time of diagnosis, which could have an impact on the future treatment of patients with PMF. We conducted a study of 131 consecutive PMF patients with median follow-up of 44 months. Data on baseline demographics, clinical and laboratory parameters, IPSS, grade of bone marrow fibrosis (MF), as well as influence of concomitant comorbidities were analyzed in terms of survival. Comorbidity was assessed using the Adult Comorbidity Evaluation-27 (ACE-27) score and the hematopoietic cell transplantation comorbidity index. An improved prognostic model of survival was obtained by deploying the MF and ACE-27 to the IPSS. A multivariable regression analyses confirmed the statistical significance of IPSS (P  lt  0.001, HR 3.754, 95 % CI 2.130-6.615), MF  gt  1 (P = 0.001, HR 2.694, 95 % CI 1.466-4.951) and ACE-27 (P  lt  0.001, HR 4.141, 95 % CI 2.322-7.386) in predicting the survival of patients with PMF. When the IPSS was modified with MF and ACE-27, the final prognostic model for overall survival was stratified as low (score 0-1), intermediate (score 2-3) and high risk (score 4-6) with median survival of not reached, 115 and 22 months, respectively (P  lt  0.001). Our findings indicate that the combination of histological changes, comorbidity assessment and clinical parameters at the time of diagnosis allows better discrimination of patients in survival prognostic groups and helps to identify high-risk patients for a poor outcome.
PB  - Humana Press Inc, Totowa
T2  - Medical Oncology
T1  - Contribution of comorbidities and grade of bone marrow fibrosis to the prognosis of survival in patients with primary myelofibrosis
IS  - 3
VL  - 31
DO  - 10.1007/s12032-014-0869-8
ER  - 

@article{
author = "Leković, Danijela and Gotić, Mirjana and Peruničić-Jovanović, Maja and Vidović, Ana and Bogdanović, Andrija and Janković, Gradimir and Čokić, Vladan and Milić, Nataša",
year = "2014",
abstract = "The widely used current International Prognostic Scoring System (IPSS) for primary myelofibrosis (PMF) is based on clinical parameters. The objective of this study was to identify additional prognostic factors at the time of diagnosis, which could have an impact on the future treatment of patients with PMF. We conducted a study of 131 consecutive PMF patients with median follow-up of 44 months. Data on baseline demographics, clinical and laboratory parameters, IPSS, grade of bone marrow fibrosis (MF), as well as influence of concomitant comorbidities were analyzed in terms of survival. Comorbidity was assessed using the Adult Comorbidity Evaluation-27 (ACE-27) score and the hematopoietic cell transplantation comorbidity index. An improved prognostic model of survival was obtained by deploying the MF and ACE-27 to the IPSS. A multivariable regression analyses confirmed the statistical significance of IPSS (P  lt  0.001, HR 3.754, 95 % CI 2.130-6.615), MF  gt  1 (P = 0.001, HR 2.694, 95 % CI 1.466-4.951) and ACE-27 (P  lt  0.001, HR 4.141, 95 % CI 2.322-7.386) in predicting the survival of patients with PMF. When the IPSS was modified with MF and ACE-27, the final prognostic model for overall survival was stratified as low (score 0-1), intermediate (score 2-3) and high risk (score 4-6) with median survival of not reached, 115 and 22 months, respectively (P  lt  0.001). Our findings indicate that the combination of histological changes, comorbidity assessment and clinical parameters at the time of diagnosis allows better discrimination of patients in survival prognostic groups and helps to identify high-risk patients for a poor outcome.",
publisher = "Humana Press Inc, Totowa",
journal = "Medical Oncology",
title = "Contribution of comorbidities and grade of bone marrow fibrosis to the prognosis of survival in patients with primary myelofibrosis",
number = "3",
volume = "31",
doi = "10.1007/s12032-014-0869-8"
}

Leković, D., Gotić, M., Peruničić-Jovanović, M., Vidović, A., Bogdanović, A., Janković, G., Čokić, V.,& Milić, N.. (2014). Contribution of comorbidities and grade of bone marrow fibrosis to the prognosis of survival in patients with primary myelofibrosis. in Medical Oncology
Humana Press Inc, Totowa., 31(3).
https://doi.org/10.1007/s12032-014-0869-8

Leković D, Gotić M, Peruničić-Jovanović M, Vidović A, Bogdanović A, Janković G, Čokić V, Milić N. Contribution of comorbidities and grade of bone marrow fibrosis to the prognosis of survival in patients with primary myelofibrosis. in Medical Oncology. 2014;31(3).
doi:10.1007/s12032-014-0869-8 .

Leković, Danijela, Gotić, Mirjana, Peruničić-Jovanović, Maja, Vidović, Ana, Bogdanović, Andrija, Janković, Gradimir, Čokić, Vladan, Milić, Nataša, "Contribution of comorbidities and grade of bone marrow fibrosis to the prognosis of survival in patients with primary myelofibrosis" in Medical Oncology, 31, no. 3 (2014),
https://doi.org/10.1007/s12032-014-0869-8 . .

TY  - CONF
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Milić, Nataša
AU  - Miljić, Predrag
AU  - Bogdanović, Andrija
AU  - Milošević, V.
AU  - Mitrović, M.
AU  - Čokić, Vladan
AU  - Elezović, Ivo
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/554
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - A prognostic model to predict thrombosis in essential thrombocythemia
EP  - 394
SP  - 394
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimi_554
ER  - 

@conference{
author = "Leković, Danijela and Gotić, Mirjana and Milić, Nataša and Miljić, Predrag and Bogdanović, Andrija and Milošević, V. and Mitrović, M. and Čokić, Vladan and Elezović, Ivo",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "A prognostic model to predict thrombosis in essential thrombocythemia",
pages = "394-394",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_rimi_554"
}

Leković, D., Gotić, M., Milić, N., Miljić, P., Bogdanović, A., Milošević, V., Mitrović, M., Čokić, V.,& Elezović, I.. (2014). A prognostic model to predict thrombosis in essential thrombocythemia. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 394-394.
https://hdl.handle.net/21.15107/rcub_rimi_554

Leković D, Gotić M, Milić N, Miljić P, Bogdanović A, Milošević V, Mitrović M, Čokić V, Elezović I. A prognostic model to predict thrombosis in essential thrombocythemia. in Haematologica. 2014;99:394-394.
https://hdl.handle.net/21.15107/rcub_rimi_554 .

Leković, Danijela, Gotić, Mirjana, Milić, Nataša, Miljić, Predrag, Bogdanović, Andrija, Milošević, V., Mitrović, M., Čokić, Vladan, Elezović, Ivo, "A prognostic model to predict thrombosis in essential thrombocythemia" in Haematologica, 99 (2014):394-394,
https://hdl.handle.net/21.15107/rcub_rimi_554 .

TY  - CONF
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Milić, Nataša
AU  - Vidović, Ana
AU  - Bogdanović, Andrija
AU  - Milošević, V.
AU  - Peruničić, Maja
AU  - Čokić, Vladan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/521
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Addition of the degree of bone marrow fibrosis to IPSS in new scoring system improve prediction of survival in primary myelofibrosis
EP  - 348
SP  - 347
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimi_521
ER  - 

@conference{
author = "Leković, Danijela and Gotić, Mirjana and Milić, Nataša and Vidović, Ana and Bogdanović, Andrija and Milošević, V. and Peruničić, Maja and Čokić, Vladan",
year = "2013",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Addition of the degree of bone marrow fibrosis to IPSS in new scoring system improve prediction of survival in primary myelofibrosis",
pages = "348-347",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_rimi_521"
}

Leković, D., Gotić, M., Milić, N., Vidović, A., Bogdanović, A., Milošević, V., Peruničić, M.,& Čokić, V.. (2013). Addition of the degree of bone marrow fibrosis to IPSS in new scoring system improve prediction of survival in primary myelofibrosis. in Haematologica
Ferrata Storti Foundation, Pavia., 98, 347-348.
https://hdl.handle.net/21.15107/rcub_rimi_521

Leković D, Gotić M, Milić N, Vidović A, Bogdanović A, Milošević V, Peruničić M, Čokić V. Addition of the degree of bone marrow fibrosis to IPSS in new scoring system improve prediction of survival in primary myelofibrosis. in Haematologica. 2013;98:347-348.
https://hdl.handle.net/21.15107/rcub_rimi_521 .

Leković, Danijela, Gotić, Mirjana, Milić, Nataša, Vidović, Ana, Bogdanović, Andrija, Milošević, V., Peruničić, Maja, Čokić, Vladan, "Addition of the degree of bone marrow fibrosis to IPSS in new scoring system improve prediction of survival in primary myelofibrosis" in Haematologica, 98 (2013):347-348,
https://hdl.handle.net/21.15107/rcub_rimi_521 .

TY  - CONF
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Bogdanović, Andrija
AU  - Miljić, Predrag
AU  - Novaković, V.
AU  - Bogunović, M.
AU  - Peruničić, Maja
AU  - Sefer, Dijana
AU  - Đorđević, V.
AU  - Čokić, Vladan
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/433
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Analyses of risk factors predicting the thrombotic complications in long-term follow up of 207 patients with essential thrombocythemia
EP  - 637
SP  - 637
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_rimi_433
ER  - 

@conference{
author = "Leković, Danijela and Gotić, Mirjana and Bogdanović, Andrija and Miljić, Predrag and Novaković, V. and Bogunović, M. and Peruničić, Maja and Sefer, Dijana and Đorđević, V. and Čokić, Vladan",
year = "2012",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Analyses of risk factors predicting the thrombotic complications in long-term follow up of 207 patients with essential thrombocythemia",
pages = "637-637",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_rimi_433"
}

Leković, D., Gotić, M., Bogdanović, A., Miljić, P., Novaković, V., Bogunović, M., Peruničić, M., Sefer, D., Đorđević, V.,& Čokić, V.. (2012). Analyses of risk factors predicting the thrombotic complications in long-term follow up of 207 patients with essential thrombocythemia. in Haematologica
Ferrata Storti Foundation, Pavia., 97, 637-637.
https://hdl.handle.net/21.15107/rcub_rimi_433

Leković D, Gotić M, Bogdanović A, Miljić P, Novaković V, Bogunović M, Peruničić M, Sefer D, Đorđević V, Čokić V. Analyses of risk factors predicting the thrombotic complications in long-term follow up of 207 patients with essential thrombocythemia. in Haematologica. 2012;97:637-637.
https://hdl.handle.net/21.15107/rcub_rimi_433 .

Leković, Danijela, Gotić, Mirjana, Bogdanović, Andrija, Miljić, Predrag, Novaković, V., Bogunović, M., Peruničić, Maja, Sefer, Dijana, Đorđević, V., Čokić, Vladan, "Analyses of risk factors predicting the thrombotic complications in long-term follow up of 207 patients with essential thrombocythemia" in Haematologica, 97 (2012):637-637,
https://hdl.handle.net/21.15107/rcub_rimi_433 .

TY  - CONF
AU  - Čokić, Vladan
AU  - Mojsilović, S.
AU  - Miloksević, V.
AU  - Kraguljac-Kurtović, Nada
AU  - Bogdanović, Andrija
AU  - Jovčić, Gordana
AU  - Milenković, P.
AU  - Gotić, Mirjana
AU  - Noguchi, Constance T.
AU  - Schechter, Alan N.
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/312
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia
EP  - 335
SP  - 334
VL  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rimi_312
ER  - 

@conference{
author = "Čokić, Vladan and Mojsilović, S. and Miloksević, V. and Kraguljac-Kurtović, Nada and Bogdanović, Andrija and Jovčić, Gordana and Milenković, P. and Gotić, Mirjana and Noguchi, Constance T. and Schechter, Alan N.",
year = "2010",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia",
pages = "335-334",
volume = "95",
url = "https://hdl.handle.net/21.15107/rcub_rimi_312"
}

Čokić, V., Mojsilović, S., Miloksević, V., Kraguljac-Kurtović, N., Bogdanović, A., Jovčić, G., Milenković, P., Gotić, M., Noguchi, C. T.,& Schechter, A. N.. (2010). Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia. in Haematologica
Ferrata Storti Foundation, Pavia., 95, 334-335.
https://hdl.handle.net/21.15107/rcub_rimi_312

Čokić V, Mojsilović S, Miloksević V, Kraguljac-Kurtović N, Bogdanović A, Jovčić G, Milenković P, Gotić M, Noguchi CT, Schechter AN. Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia. in Haematologica. 2010;95:334-335.
https://hdl.handle.net/21.15107/rcub_rimi_312 .

Čokić, Vladan, Mojsilović, S., Miloksević, V., Kraguljac-Kurtović, Nada, Bogdanović, Andrija, Jovčić, Gordana, Milenković, P., Gotić, Mirjana, Noguchi, Constance T., Schechter, Alan N., "Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia" in Haematologica, 95 (2010):334-335,
https://hdl.handle.net/21.15107/rcub_rimi_312 .