TY  - JOUR
AU  - Šefer, Dijana
AU  - Bižić-Radulović, Sandra
AU  - Kraguljac-Kurtović, Nada
AU  - Bogdanović, Andrija
AU  - Čokić, Vladan
AU  - Miljić, Predrag
AU  - Beleslin-Čokić, Bojana
AU  - Knežević, Vesna
AU  - Mitrović-Ajtić, Olivera
AU  - Leković, Danijela
AU  - Gotić, Mirjana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/795
AB  - Introduction: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are characterized by overproduction of one or more blood cell lines. Methods: We studied the proliferative characteristics of 91 patients with de novo Ph-MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34(+) cells was determined by flow cytometry. Results: The total number of PB CFC in primary myelofibrosis (PMF) was increased compared to the control sample (P  lt  0.01) and essential thrombocythemia (ET) (P  lt  0.05). The highest number of BM and PB EC was observed in polycythemia vera (PV) (P  lt  0.01). Increased levels of CD34(+) cells characterized early-prefibrotic (57%) and advanced-fibrotic PMF (90%) as compared to PV (34%) and ET (32%) (P  lt  0.01). In the whole Ph-MPN group, the total number of PB CFC (P  lt  0.01), PB EC (P  lt  0.05), and CD34+ cells (P  lt  0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD34(+) cells in PMF correlated with the total number of PB EC (P  lt  0.05) and degree of BM fibrosis (P  lt  0.01). Conclusions: Exploration of the PB proliferative characteristics of Ph-MPN on diagnosis may be helpful in revealing early-prefibrotic PMF. Monitoring the levels of circulating CD34(+) cells may provide a sensitive indicator of fibrotic evolution in PV and PMF.
PB  - Wiley, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications
EP  - 31
IS  - 1
SP  - 21
VL  - 39
DO  - 10.1111/ijlh.12564
ER  - 

@article{
author = "Šefer, Dijana and Bižić-Radulović, Sandra and Kraguljac-Kurtović, Nada and Bogdanović, Andrija and Čokić, Vladan and Miljić, Predrag and Beleslin-Čokić, Bojana and Knežević, Vesna and Mitrović-Ajtić, Olivera and Leković, Danijela and Gotić, Mirjana",
year = "2017",
abstract = "Introduction: Philadelphia-negative myeloproliferative neoplasms (Ph-MPN) are characterized by overproduction of one or more blood cell lines. Methods: We studied the proliferative characteristics of 91 patients with de novo Ph-MPN. Colony-forming cells (CFC) and endogenous colonies (EC), from bone marrow (BM) and/or peripheral blood (PB), were analyzed by colony assay based on methylcellulose. The level of circulating CD34(+) cells was determined by flow cytometry. Results: The total number of PB CFC in primary myelofibrosis (PMF) was increased compared to the control sample (P  lt  0.01) and essential thrombocythemia (ET) (P  lt  0.05). The highest number of BM and PB EC was observed in polycythemia vera (PV) (P  lt  0.01). Increased levels of CD34(+) cells characterized early-prefibrotic (57%) and advanced-fibrotic PMF (90%) as compared to PV (34%) and ET (32%) (P  lt  0.01). In the whole Ph-MPN group, the total number of PB CFC (P  lt  0.01), PB EC (P  lt  0.05), and CD34+ cells (P  lt  0.01) correlated with the degree of BM fibrosis. Higher levels of circulating CD34(+) cells in PMF correlated with the total number of PB EC (P  lt  0.05) and degree of BM fibrosis (P  lt  0.01). Conclusions: Exploration of the PB proliferative characteristics of Ph-MPN on diagnosis may be helpful in revealing early-prefibrotic PMF. Monitoring the levels of circulating CD34(+) cells may provide a sensitive indicator of fibrotic evolution in PV and PMF.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications",
pages = "31-21",
number = "1",
volume = "39",
doi = "10.1111/ijlh.12564"
}

Šefer, D., Bižić-Radulović, S., Kraguljac-Kurtović, N., Bogdanović, A., Čokić, V., Miljić, P., Beleslin-Čokić, B., Knežević, V., Mitrović-Ajtić, O., Leković, D.,& Gotić, M.. (2017). Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications. in International Journal of Laboratory Hematology
Wiley, Hoboken., 39(1), 21-31.
https://doi.org/10.1111/ijlh.12564

Šefer D, Bižić-Radulović S, Kraguljac-Kurtović N, Bogdanović A, Čokić V, Miljić P, Beleslin-Čokić B, Knežević V, Mitrović-Ajtić O, Leković D, Gotić M. Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications. in International Journal of Laboratory Hematology. 2017;39(1):21-31.
doi:10.1111/ijlh.12564 .

Šefer, Dijana, Bižić-Radulović, Sandra, Kraguljac-Kurtović, Nada, Bogdanović, Andrija, Čokić, Vladan, Miljić, Predrag, Beleslin-Čokić, Bojana, Knežević, Vesna, Mitrović-Ajtić, Olivera, Leković, Danijela, Gotić, Mirjana, "Proliferative characteristics of Philadelphia-negative myeloproliferative neoplasms - clinical implications" in International Journal of Laboratory Hematology, 39, no. 1 (2017):21-31,
https://doi.org/10.1111/ijlh.12564 . .

TY  - CONF
AU  - Sefer, Dijana
AU  - Miljić, Predrag
AU  - Kraguljac-Kurtović, Nada
AU  - Bizic-Radulović, S.
AU  - Čokić, Vladan
AU  - Bogdanović, Andrija
AU  - Marković, Dragana
AU  - Knežević, V.
AU  - Leković, Danijela
AU  - Gotić, Mirjana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/811
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms
EP  - 546
SP  - 545
VL  - 102
UR  - https://hdl.handle.net/21.15107/rcub_rimi_811
ER  - 

@conference{
author = "Sefer, Dijana and Miljić, Predrag and Kraguljac-Kurtović, Nada and Bizic-Radulović, S. and Čokić, Vladan and Bogdanović, Andrija and Marković, Dragana and Knežević, V. and Leković, Danijela and Gotić, Mirjana",
year = "2017",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms",
pages = "546-545",
volume = "102",
url = "https://hdl.handle.net/21.15107/rcub_rimi_811"
}

Sefer, D., Miljić, P., Kraguljac-Kurtović, N., Bizic-Radulović, S., Čokić, V., Bogdanović, A., Marković, D., Knežević, V., Leković, D.,& Gotić, M.. (2017). Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 102, 545-546.
https://hdl.handle.net/21.15107/rcub_rimi_811

Sefer D, Miljić P, Kraguljac-Kurtović N, Bizic-Radulović S, Čokić V, Bogdanović A, Marković D, Knežević V, Leković D, Gotić M. Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms. in Haematologica. 2017;102:545-546.
https://hdl.handle.net/21.15107/rcub_rimi_811 .

Sefer, Dijana, Miljić, Predrag, Kraguljac-Kurtović, Nada, Bizic-Radulović, S., Čokić, Vladan, Bogdanović, Andrija, Marković, Dragana, Knežević, V., Leković, Danijela, Gotić, Mirjana, "Monitoring of leukocyte-platelet aggregates and selectin levels in patients with philadelphia-negative myeloproliferative neoplasms" in Haematologica, 102 (2017):545-546,
https://hdl.handle.net/21.15107/rcub_rimi_811 .