Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress
Само за регистроване кориснике
2020
Аутори
Vignjević Petrinović, SanjaBudeč, Mirela
Marković, Dragana
Mitrović-Ajtić, Olivera
Jovčić, Gordana
Milošević, Maja
Momčilović, Sanja
Čokić, Vladan
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress expos...ure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients.
Кључне речи:
Bone marrow / Erythroid progenitors / Nitric oxide / StressИзвор:
Histochemistry & Cell Biology, 2020, 153, 6, 457-468Издавач:
- Springer, New York
Финансирање / пројекти:
- Испитивање патогенезе хематолошких малигнитета (RS-MESTD-Basic Research (BR or ON)-175053)
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
DOI: 10.1007/s00418-020-01856-y
ISSN: 0948-6143
PubMed: 32144481
WoS: 000518343400001
Scopus: 2-s2.0-85081622035
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Vignjević Petrinović, Sanja AU - Budeč, Mirela AU - Marković, Dragana AU - Mitrović-Ajtić, Olivera AU - Jovčić, Gordana AU - Milošević, Maja AU - Momčilović, Sanja AU - Čokić, Vladan PY - 2020 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1029 AB - Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress exposure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients. PB - Springer, New York T2 - Histochemistry & Cell Biology T1 - Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress EP - 468 IS - 6 SP - 457 VL - 153 DO - 10.1007/s00418-020-01856-y ER -
@article{ author = "Vignjević Petrinović, Sanja and Budeč, Mirela and Marković, Dragana and Mitrović-Ajtić, Olivera and Jovčić, Gordana and Milošević, Maja and Momčilović, Sanja and Čokić, Vladan", year = "2020", abstract = "Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress exposure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients.", publisher = "Springer, New York", journal = "Histochemistry & Cell Biology", title = "Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress", pages = "468-457", number = "6", volume = "153", doi = "10.1007/s00418-020-01856-y" }
Vignjević Petrinović, S., Budeč, M., Marković, D., Mitrović-Ajtić, O., Jovčić, G., Milošević, M., Momčilović, S.,& Čokić, V.. (2020). Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress. in Histochemistry & Cell Biology Springer, New York., 153(6), 457-468. https://doi.org/10.1007/s00418-020-01856-y
Vignjević Petrinović S, Budeč M, Marković D, Mitrović-Ajtić O, Jovčić G, Milošević M, Momčilović S, Čokić V. Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress. in Histochemistry & Cell Biology. 2020;153(6):457-468. doi:10.1007/s00418-020-01856-y .
Vignjević Petrinović, Sanja, Budeč, Mirela, Marković, Dragana, Mitrović-Ajtić, Olivera, Jovčić, Gordana, Milošević, Maja, Momčilović, Sanja, Čokić, Vladan, "Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress" in Histochemistry & Cell Biology, 153, no. 6 (2020):457-468, https://doi.org/10.1007/s00418-020-01856-y . .