TY  - JOUR
AU  - Ušanović, Lejla
AU  - Lasić, Lejla
AU  - Hanjalić, Jasna
AU  - Ćakić, Sanja
AU  - Destanović, Dalila
AU  - Kalamujić Stroil, Belma
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1165
AB  - Since ticks have been recognized as one of the most important vectors of pathogens causing serious diseases in humans, a number of studies have focused on identifying the pathogen composition as well as transmission and infection mechanisms. Although a plethora of detection methods is available today, PCR-based approach is regarded as the most sensitive and rapid. However, common challenges in molecular analyses conducted on ticks are weak amplification results because of present inhibitors, either from a mammalian bloodmeal or a male DNA in female reproductive organs. Present study aimed to evaluate which body part of an engorged tick is the most preferable as a starting material in DNA extraction for molecular detection of Borrelia burgdorferi sensu lato, causative agent of Lyme borreliosis. We analyzed 58 Ixodes ricinus ticks removed from patients in The Center for Emergency Medical Assistance of the Sarajevo Canton, Bosnia and Herzegovina. Our findings suggest using the anterior half of semi-engorged and fully-engorged ticks for DNA extraction with the purpose of Borrelia detection.
PB  - Acarological Society of Iran
T2  - Persian Journal of Acarology
T1  - Determination of an efficient and reliable method for PCR detection of borrelial DNA from engorged ticks
EP  - 230
IS  - 2
SP  - 223
VL  - 10
DO  - 10.22073/pja.v10i2.66704
ER  - 

@article{
author = "Ušanović, Lejla and Lasić, Lejla and Hanjalić, Jasna and Ćakić, Sanja and Destanović, Dalila and Kalamujić Stroil, Belma",
year = "2021",
abstract = "Since ticks have been recognized as one of the most important vectors of pathogens causing serious diseases in humans, a number of studies have focused on identifying the pathogen composition as well as transmission and infection mechanisms. Although a plethora of detection methods is available today, PCR-based approach is regarded as the most sensitive and rapid. However, common challenges in molecular analyses conducted on ticks are weak amplification results because of present inhibitors, either from a mammalian bloodmeal or a male DNA in female reproductive organs. Present study aimed to evaluate which body part of an engorged tick is the most preferable as a starting material in DNA extraction for molecular detection of Borrelia burgdorferi sensu lato, causative agent of Lyme borreliosis. We analyzed 58 Ixodes ricinus ticks removed from patients in The Center for Emergency Medical Assistance of the Sarajevo Canton, Bosnia and Herzegovina. Our findings suggest using the anterior half of semi-engorged and fully-engorged ticks for DNA extraction with the purpose of Borrelia detection.",
publisher = "Acarological Society of Iran",
journal = "Persian Journal of Acarology",
title = "Determination of an efficient and reliable method for PCR detection of borrelial DNA from engorged ticks",
pages = "230-223",
number = "2",
volume = "10",
doi = "10.22073/pja.v10i2.66704"
}

Ušanović, L., Lasić, L., Hanjalić, J., Ćakić, S., Destanović, D.,& Kalamujić Stroil, B.. (2021). Determination of an efficient and reliable method for PCR detection of borrelial DNA from engorged ticks. in Persian Journal of Acarology
Acarological Society of Iran., 10(2), 223-230.
https://doi.org/10.22073/pja.v10i2.66704

Ušanović L, Lasić L, Hanjalić J, Ćakić S, Destanović D, Kalamujić Stroil B. Determination of an efficient and reliable method for PCR detection of borrelial DNA from engorged ticks. in Persian Journal of Acarology. 2021;10(2):223-230.
doi:10.22073/pja.v10i2.66704 .

Ušanović, Lejla, Lasić, Lejla, Hanjalić, Jasna, Ćakić, Sanja, Destanović, Dalila, Kalamujić Stroil, Belma, "Determination of an efficient and reliable method for PCR detection of borrelial DNA from engorged ticks" in Persian Journal of Acarology, 10, no. 2 (2021):223-230,
https://doi.org/10.22073/pja.v10i2.66704 . .

TY  - JOUR
AU  - Veinović, Gorana
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Tomanović, Snežana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1044
AB  - Barbour-Stoenner-Kelly II (BSK-II) and BSK-H media were used for cultivation and isolation of fastidiousBorreliaspecies - the causative agents of Lyme borreliosis. Culture media have a limited shelf life and require adequate storage. Our goal was to assess how the growth ofBorreliawould be affected by prolonged storage of media and inadequate storage conditions (BSK-H stored at +4 degrees C for 2.5 years and BSK-II stored at -20 degrees C for 11 years). Growth of differentBorrelia afzelii,Borrelia garinii,Borrelia lusitaniaeandBorrelia valaisianastrains was assessed during 2 weeks of incubation at 33 degrees C. Monitored parameters included cell count per mL, morphology and motility. The results of this study have shown weaker growth of borrelia strains in BSK-H at +4 degrees C (median final cell number of 1.5 x 10(6)/mL) than in BSK-II at -20 degrees C (median final cell number of 7.75 x 10(6)/mL) and in fresh BSK-H media (median final cell number of 8.95 x 10(6)/mL). Duration of storage of media had no impact onBorreliamorphology and motility. Our results indicate that temperature of -20 degrees C is optimal for long-term storage of medium, BSK-II stored for 11 years provided effective support to growth ofBorreliaand may be employed for cultivation.
PB  - Wiley, Hoboken
T2  - APMIS
T1  - Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods
EP  - 557
IS  - 10
SP  - 552
VL  - 128
DO  - 10.1111/apm.13069
ER  - 

@article{
author = "Veinović, Gorana and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Tomanović, Snežana",
year = "2020",
abstract = "Barbour-Stoenner-Kelly II (BSK-II) and BSK-H media were used for cultivation and isolation of fastidiousBorreliaspecies - the causative agents of Lyme borreliosis. Culture media have a limited shelf life and require adequate storage. Our goal was to assess how the growth ofBorreliawould be affected by prolonged storage of media and inadequate storage conditions (BSK-H stored at +4 degrees C for 2.5 years and BSK-II stored at -20 degrees C for 11 years). Growth of differentBorrelia afzelii,Borrelia garinii,Borrelia lusitaniaeandBorrelia valaisianastrains was assessed during 2 weeks of incubation at 33 degrees C. Monitored parameters included cell count per mL, morphology and motility. The results of this study have shown weaker growth of borrelia strains in BSK-H at +4 degrees C (median final cell number of 1.5 x 10(6)/mL) than in BSK-II at -20 degrees C (median final cell number of 7.75 x 10(6)/mL) and in fresh BSK-H media (median final cell number of 8.95 x 10(6)/mL). Duration of storage of media had no impact onBorreliamorphology and motility. Our results indicate that temperature of -20 degrees C is optimal for long-term storage of medium, BSK-II stored for 11 years provided effective support to growth ofBorreliaand may be employed for cultivation.",
publisher = "Wiley, Hoboken",
journal = "APMIS",
title = "Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods",
pages = "557-552",
number = "10",
volume = "128",
doi = "10.1111/apm.13069"
}

Veinović, G., Ćakić, S., Mihaljica, D., Sukara, R.,& Tomanović, S.. (2020). Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods. in APMIS
Wiley, Hoboken., 128(10), 552-557.
https://doi.org/10.1111/apm.13069

Veinović G, Ćakić S, Mihaljica D, Sukara R, Tomanović S. Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods. in APMIS. 2020;128(10):552-557.
doi:10.1111/apm.13069 .

Veinović, Gorana, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Tomanović, Snežana, "Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods" in APMIS, 128, no. 10 (2020):552-557,
https://doi.org/10.1111/apm.13069 . .

TY  - CONF
AU  - Veinović, Gorana
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Tomanović, Snežana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1414
PB  - Federation of European Microbiological Societies (FEMS)
C3  - FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book
T1  - Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis
SP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1414
ER  - 

@conference{
author = "Veinović, Gorana and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Tomanović, Snežana",
year = "2020",
publisher = "Federation of European Microbiological Societies (FEMS)",
journal = "FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book",
title = "Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis",
pages = "220",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1414"
}

Veinović, G., Ćakić, S., Mihaljica, D., Sukara, R.,& Tomanović, S.. (2020). Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis. in FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book
Federation of European Microbiological Societies (FEMS)., 220.
https://hdl.handle.net/21.15107/rcub_rimi_1414

Veinović G, Ćakić S, Mihaljica D, Sukara R, Tomanović S. Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis. in FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book. 2020;:220.
https://hdl.handle.net/21.15107/rcub_rimi_1414 .

Veinović, Gorana, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Tomanović, Snežana, "Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis" in FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book (2020):220,
https://hdl.handle.net/21.15107/rcub_rimi_1414 .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Veinović, Gorana
AU  - Cerar, T.
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ruzic-Sabljić, E.
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/934
AB  - Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.
PB  - Wiley, Hoboken
T2  - Medical & Veterinary Entomology
T1  - Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia
EP  - 520
IS  - 4
SP  - 512
VL  - 33
DO  - 10.1111/mve.12392
ER  - 

@article{
author = "Ćakić, Sanja and Veinović, Gorana and Cerar, T. and Mihaljica, Darko and Sukara, Ratko and Ruzic-Sabljić, E. and Tomanović, Snežana",
year = "2019",
abstract = "Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.",
publisher = "Wiley, Hoboken",
journal = "Medical & Veterinary Entomology",
title = "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia",
pages = "520-512",
number = "4",
volume = "33",
doi = "10.1111/mve.12392"
}

Ćakić, S., Veinović, G., Cerar, T., Mihaljica, D., Sukara, R., Ruzic-Sabljić, E.,& Tomanović, S.. (2019). Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology
Wiley, Hoboken., 33(4), 512-520.
https://doi.org/10.1111/mve.12392

Ćakić S, Veinović G, Cerar T, Mihaljica D, Sukara R, Ruzic-Sabljić E, Tomanović S. Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology. 2019;33(4):512-520.
doi:10.1111/mve.12392 .

Ćakić, Sanja, Veinović, Gorana, Cerar, T., Mihaljica, Darko, Sukara, Ratko, Ruzic-Sabljić, E., Tomanović, Snežana, "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia" in Medical & Veterinary Entomology, 33, no. 4 (2019):512-520,
https://doi.org/10.1111/mve.12392 . .

TY  - CONF
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ćakić, Sanja
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1411
PB  - Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)
C3  - International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts
T1  - Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia
EP  - 112
SP  - P77(112)
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1411
ER  - 

@conference{
author = "Veinović, Gorana and Mihaljica, Darko and Sukara, Ratko and Ćakić, Sanja and Tomanović, Snežana",
year = "2019",
publisher = "Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)",
journal = "International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts",
title = "Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia",
pages = "112-P77(112)",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1411"
}

Veinović, G., Mihaljica, D., Sukara, R., Ćakić, S.,& Tomanović, S.. (2019). Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia. in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts
Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)., P77(112)-112.
https://hdl.handle.net/21.15107/rcub_rimi_1411

Veinović G, Mihaljica D, Sukara R, Ćakić S, Tomanović S. Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia. in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts. 2019;:P77(112)-112.
https://hdl.handle.net/21.15107/rcub_rimi_1411 .

Veinović, Gorana, Mihaljica, Darko, Sukara, Ratko, Ćakić, Sanja, Tomanović, Snežana, "Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia" in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts (2019):P77(112)-112,
https://hdl.handle.net/21.15107/rcub_rimi_1411 .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Chochlakis, Dimosthenis
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Ćakić, Sanja
AU  - Valcić, Miroslav
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
AU  - Tomanović, Snežana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/893
AB  - The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia. Ticks, when present, were removed, and after necropsy, spleen samples were collected from each animal. All tick and spleen samples were tested for the DNA of bacterial and protozoan tick-borne pathogens (Borrelia species, Bartonella species, Rickettsia species, Anaplasma species, Coxiella burnetii, Francisella species and Babesia species) by multiplex real-time PCR, conventional PCR and sequencing analyses. The DNA of Babesia canis was detected in nine out of 216 (4.2%) spleen samples, and two samples (0.9%) tested positive for Anaplasma phagocytophilum. In 118 ticks collected from jackals, the DNA of two Babesia species (Ba. canis and Ba. microti), three Borrelia species (Bo. garinii, Bo. valaisiana, and Bo. lusitaniae) and A. marginale was detected. From the aspect of public health surveillance, the potential role of the golden jackal in the maintenance of vector-borne zoonotic pathogens in Serbia must be considered, and further eco-epidemiological studies should be performed to determine the precise role of this animal species in zoonotic disease transmission cycles.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks & Tick-Borne Diseases
T1  - Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia
EP  - 1097
IS  - 5
SP  - 1090
VL  - 9
DO  - 10.1016/j.ttbdis.2018.04.003
ER  - 

@article{
author = "Sukara, Ratko and Chochlakis, Dimosthenis and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Ćakić, Sanja and Valcić, Miroslav and Tselentis, Yannis and Psaroulaki, Anna and Tomanović, Snežana",
year = "2018",
abstract = "The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia. Ticks, when present, were removed, and after necropsy, spleen samples were collected from each animal. All tick and spleen samples were tested for the DNA of bacterial and protozoan tick-borne pathogens (Borrelia species, Bartonella species, Rickettsia species, Anaplasma species, Coxiella burnetii, Francisella species and Babesia species) by multiplex real-time PCR, conventional PCR and sequencing analyses. The DNA of Babesia canis was detected in nine out of 216 (4.2%) spleen samples, and two samples (0.9%) tested positive for Anaplasma phagocytophilum. In 118 ticks collected from jackals, the DNA of two Babesia species (Ba. canis and Ba. microti), three Borrelia species (Bo. garinii, Bo. valaisiana, and Bo. lusitaniae) and A. marginale was detected. From the aspect of public health surveillance, the potential role of the golden jackal in the maintenance of vector-borne zoonotic pathogens in Serbia must be considered, and further eco-epidemiological studies should be performed to determine the precise role of this animal species in zoonotic disease transmission cycles.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks & Tick-Borne Diseases",
title = "Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia",
pages = "1097-1090",
number = "5",
volume = "9",
doi = "10.1016/j.ttbdis.2018.04.003"
}

Sukara, R., Chochlakis, D., Ćirović, D., Penezić, A., Mihaljica, D., Ćakić, S., Valcić, M., Tselentis, Y., Psaroulaki, A.,& Tomanović, S.. (2018). Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 9(5), 1090-1097.
https://doi.org/10.1016/j.ttbdis.2018.04.003

Sukara R, Chochlakis D, Ćirović D, Penezić A, Mihaljica D, Ćakić S, Valcić M, Tselentis Y, Psaroulaki A, Tomanović S. Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia. in Ticks & Tick-Borne Diseases. 2018;9(5):1090-1097.
doi:10.1016/j.ttbdis.2018.04.003 .

Sukara, Ratko, Chochlakis, Dimosthenis, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Ćakić, Sanja, Valcić, Miroslav, Tselentis, Yannis, Psaroulaki, Anna, Tomanović, Snežana, "Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia" in Ticks & Tick-Borne Diseases, 9, no. 5 (2018):1090-1097,
https://doi.org/10.1016/j.ttbdis.2018.04.003 . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Željko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Milanović, Zorana
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/762
AB  - Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts
EP  - 437
IS  - 4
SP  - 429
VL  - 72
DO  - 10.1007/s10493-017-0170-6
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Željko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Milanović, Zorana and Tomanović, Snežana",
year = "2017",
abstract = "Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts",
pages = "437-429",
number = "4",
volume = "72",
doi = "10.1007/s10493-017-0170-6"
}

Mihaljica, D., Marković, D., Radulović, Ž., Mulenga, A., Ćakić, S., Sukara, R., Milanović, Z.,& Tomanović, S.. (2017). Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology
Springer, Dordrecht., 72(4), 429-437.
https://doi.org/10.1007/s10493-017-0170-6

Mihaljica D, Marković D, Radulović Ž, Mulenga A, Ćakić S, Sukara R, Milanović Z, Tomanović S. Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology. 2017;72(4):429-437.
doi:10.1007/s10493-017-0170-6 .

Mihaljica, Darko, Marković, Dragana, Radulović, Željko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Milanović, Zorana, Tomanović, Snežana, "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts" in Experimental & Applied Acarology, 72, no. 4 (2017):429-437,
https://doi.org/10.1007/s10493-017-0170-6 . .

TY  - JOUR
AU  - Radulović, Željko
AU  - Mihaljica, Darko
AU  - Ćosić, Nada
AU  - Penezić, Aleksandra
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/777
AB  - Specimens of the endangered species Spermophilus citellus (European ground squirrel) from populations inhabiting 12 localities in Vojvodina (Serbia) were inspected for the presence of attached hard ticks. A total of 1009 ticks were found on 151 infested animals. With respect to the level of infestation, over 60% of the squirrels were found with five or less ticks attached, while the distribution of the number of animals with a higher level of infestation tended to follow the 80/20 rule. A notably higher level of infestation was detected in males as compared to females. Nymphs were the predominant developmental stage of ticks parasitizing the inspected European ground squirrel specimens. The majority of removed ticks belonged to the nidicolous species Ixodes laguri (79%), while the remaining ticks were identified as Haemaphysalis concinna. In seeking molecular confirmation of morphological identification of Ixodes specimens, COI gene sequences of I. laguri were obtained and deposited in GenBank.
PB  - Inst Zoology, Bas, Sofia
T2  - Acta Zoologica Bulgarica
T1  - Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia
EP  - 553
IS  - 4
SP  - 547
VL  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimi_777
ER  - 

@article{
author = "Radulović, Željko and Mihaljica, Darko and Ćosić, Nada and Penezić, Aleksandra and Ćakić, Sanja and Sukara, Ratko and Ćirović, Duško and Tomanović, Snežana",
year = "2017",
abstract = "Specimens of the endangered species Spermophilus citellus (European ground squirrel) from populations inhabiting 12 localities in Vojvodina (Serbia) were inspected for the presence of attached hard ticks. A total of 1009 ticks were found on 151 infested animals. With respect to the level of infestation, over 60% of the squirrels were found with five or less ticks attached, while the distribution of the number of animals with a higher level of infestation tended to follow the 80/20 rule. A notably higher level of infestation was detected in males as compared to females. Nymphs were the predominant developmental stage of ticks parasitizing the inspected European ground squirrel specimens. The majority of removed ticks belonged to the nidicolous species Ixodes laguri (79%), while the remaining ticks were identified as Haemaphysalis concinna. In seeking molecular confirmation of morphological identification of Ixodes specimens, COI gene sequences of I. laguri were obtained and deposited in GenBank.",
publisher = "Inst Zoology, Bas, Sofia",
journal = "Acta Zoologica Bulgarica",
title = "Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia",
pages = "553-547",
number = "4",
volume = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimi_777"
}

Radulović, Ž., Mihaljica, D., Ćosić, N., Penezić, A., Ćakić, S., Sukara, R., Ćirović, D.,& Tomanović, S.. (2017). Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia. in Acta Zoologica Bulgarica
Inst Zoology, Bas, Sofia., 69(4), 547-553.
https://hdl.handle.net/21.15107/rcub_rimi_777

Radulović Ž, Mihaljica D, Ćosić N, Penezić A, Ćakić S, Sukara R, Ćirović D, Tomanović S. Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia. in Acta Zoologica Bulgarica. 2017;69(4):547-553.
https://hdl.handle.net/21.15107/rcub_rimi_777 .

Radulović, Željko, Mihaljica, Darko, Ćosić, Nada, Penezić, Aleksandra, Ćakić, Sanja, Sukara, Ratko, Ćirović, Duško, Tomanović, Snežana, "Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia" in Acta Zoologica Bulgarica, 69, no. 4 (2017):547-553,
https://hdl.handle.net/21.15107/rcub_rimi_777 .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Eljko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Samardzić, Jelena
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/816
AB  - Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks & Tick-Borne Diseases
T1  - Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation
EP  - 395
IS  - 3
SP  - 391
VL  - 8
DO  - 10.1016/j.ttbdis.2017.01.001
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Eljko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Samardzić, Jelena and Tomanović, Snežana",
year = "2017",
abstract = "Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks & Tick-Borne Diseases",
title = "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation",
pages = "395-391",
number = "3",
volume = "8",
doi = "10.1016/j.ttbdis.2017.01.001"
}

Mihaljica, D., Marković, D., Radulović, E., Mulenga, A., Ćakić, S., Sukara, R., Samardzić, J.,& Tomanović, S.. (2017). Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 8(3), 391-395.
https://doi.org/10.1016/j.ttbdis.2017.01.001

Mihaljica D, Marković D, Radulović E, Mulenga A, Ćakić S, Sukara R, Samardzić J, Tomanović S. Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases. 2017;8(3):391-395.
doi:10.1016/j.ttbdis.2017.01.001 .

Mihaljica, Darko, Marković, Dragana, Radulović, Eljko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Samardzić, Jelena, Tomanović, Snežana, "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation" in Ticks & Tick-Borne Diseases, 8, no. 3 (2017):391-395,
https://doi.org/10.1016/j.ttbdis.2017.01.001 . .

TY  - JOUR
AU  - Burazerović, Jelena
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/654
AB  - Ticks parasitizing bats have been largely understudied, especially in the central part of the Balkan Peninsula, where the last data from the field research date from almost 25 years ago. Bats are hosts to a large number of ectoparasites, including ticks, which can act as vectors of zoonotic agents. For this reason, it is important to identify the distribution of ticks and their relationship to different hosts, including wild animals, bats in particular. The present research was conducted at 16 localities throughout Serbia, Montenegro, Bosnia and Herzegovina, and Former Yugoslav Republic of Macedonia (FYROM). We examined 475 individuals of bats belonging to 13 species. A total of three tick species were identified, I. simplex being the most numerous and widespread, followed by I. vespertilionis and A. vespertilionis. To the best of our knowledge, the presented data include the first records of I. simplex in Serbia and Montenegro, I. vespertilionis for Montenegro and A. vespertilionis in FYROM. Also, we identify a new possible host/parasite association between I. simplex and Rhinolophus euryale.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans
EP  - 291
IS  - 2
SP  - 281
VL  - 66
DO  - 10.1007/s10493-015-9891-6
ER  - 

@article{
author = "Burazerović, Jelena and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Ćirović, Duško and Tomanović, Snežana",
year = "2015",
abstract = "Ticks parasitizing bats have been largely understudied, especially in the central part of the Balkan Peninsula, where the last data from the field research date from almost 25 years ago. Bats are hosts to a large number of ectoparasites, including ticks, which can act as vectors of zoonotic agents. For this reason, it is important to identify the distribution of ticks and their relationship to different hosts, including wild animals, bats in particular. The present research was conducted at 16 localities throughout Serbia, Montenegro, Bosnia and Herzegovina, and Former Yugoslav Republic of Macedonia (FYROM). We examined 475 individuals of bats belonging to 13 species. A total of three tick species were identified, I. simplex being the most numerous and widespread, followed by I. vespertilionis and A. vespertilionis. To the best of our knowledge, the presented data include the first records of I. simplex in Serbia and Montenegro, I. vespertilionis for Montenegro and A. vespertilionis in FYROM. Also, we identify a new possible host/parasite association between I. simplex and Rhinolophus euryale.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans",
pages = "291-281",
number = "2",
volume = "66",
doi = "10.1007/s10493-015-9891-6"
}

Burazerović, J., Ćakić, S., Mihaljica, D., Sukara, R., Ćirović, D.,& Tomanović, S.. (2015). Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans. in Experimental & Applied Acarology
Springer, Dordrecht., 66(2), 281-291.
https://doi.org/10.1007/s10493-015-9891-6

Burazerović J, Ćakić S, Mihaljica D, Sukara R, Ćirović D, Tomanović S. Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans. in Experimental & Applied Acarology. 2015;66(2):281-291.
doi:10.1007/s10493-015-9891-6 .

Burazerović, Jelena, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Ćirović, Duško, Tomanović, Snežana, "Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans" in Experimental & Applied Acarology, 66, no. 2 (2015):281-291,
https://doi.org/10.1007/s10493-015-9891-6 . .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Mojsilović, Miljana
AU  - Mihaljica, Darko
AU  - Milutinović, Marija
AU  - Petrović, Anđeljko
AU  - Tomanović, Snežana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/551
AB  - The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia
EP  - 690
IS  - 2
SP  - 683
VL  - 66
DO  - 10.2298/ABS1402683C
ER  - 

@article{
author = "Ćakić, Sanja and Mojsilović, Miljana and Mihaljica, Darko and Milutinović, Marija and Petrović, Anđeljko and Tomanović, Snežana",
year = "2014",
abstract = "The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia",
pages = "690-683",
number = "2",
volume = "66",
doi = "10.2298/ABS1402683C"
}

Ćakić, S., Mojsilović, M., Mihaljica, D., Milutinović, M., Petrović, A.,& Tomanović, S.. (2014). Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 683-690.
https://doi.org/10.2298/ABS1402683C

Ćakić S, Mojsilović M, Mihaljica D, Milutinović M, Petrović A, Tomanović S. Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia. in Archives of Biological Sciences. 2014;66(2):683-690.
doi:10.2298/ABS1402683C .

Ćakić, Sanja, Mojsilović, Miljana, Mihaljica, Darko, Milutinović, Marija, Petrović, Anđeljko, Tomanović, Snežana, "Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia" in Archives of Biological Sciences, 66, no. 2 (2014):683-690,
https://doi.org/10.2298/ABS1402683C . .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Mojsilović, Miljana
AU  - Mihaljica, Darko
AU  - Milutinović, Marija
AU  - Petrović, Anđeljko
AU  - Tomanović, Snežana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/578
AB  - The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia
EP  - 1251
IS  - 3
SP  - 1243
VL  - 66
DO  - 10.2298/ABS1403243C
ER  - 

@article{
author = "Ćakić, Sanja and Mojsilović, Miljana and Mihaljica, Darko and Milutinović, Marija and Petrović, Anđeljko and Tomanović, Snežana",
year = "2014",
abstract = "The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia",
pages = "1251-1243",
number = "3",
volume = "66",
doi = "10.2298/ABS1403243C"
}

Ćakić, S., Mojsilović, M., Mihaljica, D., Milutinović, M., Petrović, A.,& Tomanović, S.. (2014). Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(3), 1243-1251.
https://doi.org/10.2298/ABS1403243C

Ćakić S, Mojsilović M, Mihaljica D, Milutinović M, Petrović A, Tomanović S. Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia. in Archives of Biological Sciences. 2014;66(3):1243-1251.
doi:10.2298/ABS1403243C .

Ćakić, Sanja, Mojsilović, Miljana, Mihaljica, Darko, Milutinović, Marija, Petrović, Anđeljko, Tomanović, Snežana, "Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia" in Archives of Biological Sciences, 66, no. 3 (2014):1243-1251,
https://doi.org/10.2298/ABS1403243C . .

TY  - JOUR
AU  - Tomanović, Snežana
AU  - Chochlakis, Dimosthenis
AU  - Radulović, Željko
AU  - Milutinović, Marija
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/516
AB  - Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia
EP  - 376
IS  - 3
SP  - 367
VL  - 59
DO  - 10.1007/s10493-012-9597-y
ER  - 

@article{
author = "Tomanović, Snežana and Chochlakis, Dimosthenis and Radulović, Željko and Milutinović, Marija and Ćakić, Sanja and Mihaljica, Darko and Tselentis, Yannis and Psaroulaki, Anna",
year = "2013",
abstract = "Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia",
pages = "376-367",
number = "3",
volume = "59",
doi = "10.1007/s10493-012-9597-y"
}

Tomanović, S., Chochlakis, D., Radulović, Ž., Milutinović, M., Ćakić, S., Mihaljica, D., Tselentis, Y.,& Psaroulaki, A.. (2013). Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia. in Experimental & Applied Acarology
Springer, Dordrecht., 59(3), 367-376.
https://doi.org/10.1007/s10493-012-9597-y

Tomanović S, Chochlakis D, Radulović Ž, Milutinović M, Ćakić S, Mihaljica D, Tselentis Y, Psaroulaki A. Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia. in Experimental & Applied Acarology. 2013;59(3):367-376.
doi:10.1007/s10493-012-9597-y .

Tomanović, Snežana, Chochlakis, Dimosthenis, Radulović, Željko, Milutinović, Marija, Ćakić, Sanja, Mihaljica, Darko, Tselentis, Yannis, Psaroulaki, Anna, "Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia" in Experimental & Applied Acarology, 59, no. 3 (2013):367-376,
https://doi.org/10.1007/s10493-012-9597-y . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Radulović, Željko
AU  - Tomanović, Snežana
AU  - Ćakić, Sanja
AU  - Penezić, Aleksandra
AU  - Milutinović, Marija
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/407
AB  - In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Molecular detection of Babesia spp. in ticks in northern Serbia
EP  - 1598
IS  - 4
SP  - 1591
VL  - 64
DO  - 10.2298/ABS1204591M
ER  - 

@article{
author = "Mihaljica, Darko and Radulović, Željko and Tomanović, Snežana and Ćakić, Sanja and Penezić, Aleksandra and Milutinović, Marija",
year = "2012",
abstract = "In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular detection of Babesia spp. in ticks in northern Serbia",
pages = "1598-1591",
number = "4",
volume = "64",
doi = "10.2298/ABS1204591M"
}

Mihaljica, D., Radulović, Ž., Tomanović, S., Ćakić, S., Penezić, A.,& Milutinović, M.. (2012). Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1591-1598.
https://doi.org/10.2298/ABS1204591M

Mihaljica D, Radulović Ž, Tomanović S, Ćakić S, Penezić A, Milutinović M. Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences. 2012;64(4):1591-1598.
doi:10.2298/ABS1204591M .

Mihaljica, Darko, Radulović, Željko, Tomanović, Snežana, Ćakić, Sanja, Penezić, Aleksandra, Milutinović, Marija, "Molecular detection of Babesia spp. in ticks in northern Serbia" in Archives of Biological Sciences, 64, no. 4 (2012):1591-1598,
https://doi.org/10.2298/ABS1204591M . .

TY  - JOUR
AU  - Radulović, Željko
AU  - Milutinović, Marija
AU  - Tomanović, Snežana
AU  - Mihaljica, Darko
AU  - Ćakić, Sanja
AU  - Stamenkovic-Radak, M.
AU  - Anđelković, M.
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/413
AB  - Previous results indicate that glycerol-3-phosphate dehydrogenase variability represents the adaptation of Ixodes ricinus L. (Acari: Ixodidae) to fluctuations of environmental conditions, particularly to temperature. Analysis of crucial polymorphisms in I. ricinus Gpdh gene was done by the restriction method, and three different haplotypes were obtained (GPDH(441) 1, GPDH(441) 2, and GPDH(441) 3), corresponding to GPDH alleles detected by allozyme electrophoresis. Differences in GPDH(441) haplotype and genotype frequencies were found between samples from open and forest habitats. Significant seasonal variations of GPDH(441) haplotype and genotype frequencies were detected in samples from the open habitats. No seasonal variations were observed at forest localities, probably because of the less pronounced amplitude of environmental factors. The possible role of host availability was discussed as an important factor that affects seasonal dynamics and genetic composition of tick populations.
PB  - Entomological Soc Amer, Lanham
T2  - Journal of Medical Entomology
T1  - Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability in Ixodes ricinus (Acari: Ixodidae) Populations
EP  - 503
IS  - 3
SP  - 497
VL  - 49
DO  - 10.1603/ME11101
ER  - 

@article{
author = "Radulović, Željko and Milutinović, Marija and Tomanović, Snežana and Mihaljica, Darko and Ćakić, Sanja and Stamenkovic-Radak, M. and Anđelković, M.",
year = "2012",
abstract = "Previous results indicate that glycerol-3-phosphate dehydrogenase variability represents the adaptation of Ixodes ricinus L. (Acari: Ixodidae) to fluctuations of environmental conditions, particularly to temperature. Analysis of crucial polymorphisms in I. ricinus Gpdh gene was done by the restriction method, and three different haplotypes were obtained (GPDH(441) 1, GPDH(441) 2, and GPDH(441) 3), corresponding to GPDH alleles detected by allozyme electrophoresis. Differences in GPDH(441) haplotype and genotype frequencies were found between samples from open and forest habitats. Significant seasonal variations of GPDH(441) haplotype and genotype frequencies were detected in samples from the open habitats. No seasonal variations were observed at forest localities, probably because of the less pronounced amplitude of environmental factors. The possible role of host availability was discussed as an important factor that affects seasonal dynamics and genetic composition of tick populations.",
publisher = "Entomological Soc Amer, Lanham",
journal = "Journal of Medical Entomology",
title = "Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability in Ixodes ricinus (Acari: Ixodidae) Populations",
pages = "503-497",
number = "3",
volume = "49",
doi = "10.1603/ME11101"
}

Radulović, Ž., Milutinović, M., Tomanović, S., Mihaljica, D., Ćakić, S., Stamenkovic-Radak, M.,& Anđelković, M.. (2012). Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability in Ixodes ricinus (Acari: Ixodidae) Populations. in Journal of Medical Entomology
Entomological Soc Amer, Lanham., 49(3), 497-503.
https://doi.org/10.1603/ME11101

Radulović Ž, Milutinović M, Tomanović S, Mihaljica D, Ćakić S, Stamenkovic-Radak M, Anđelković M. Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability in Ixodes ricinus (Acari: Ixodidae) Populations. in Journal of Medical Entomology. 2012;49(3):497-503.
doi:10.1603/ME11101 .

Radulović, Željko, Milutinović, Marija, Tomanović, Snežana, Mihaljica, Darko, Ćakić, Sanja, Stamenkovic-Radak, M., Anđelković, M., "Seasonal and Spatial Occurrence of Glycerol-3-Phosphate Dehydrogenase Variability in Ixodes ricinus (Acari: Ixodidae) Populations" in Journal of Medical Entomology, 49, no. 3 (2012):497-503,
https://doi.org/10.1603/ME11101 . .