TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Gotić, Mirjana
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
AU  - Stošić-Grujičić, Stanislava
AU  - Ivanov, Milan
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/745
AB  - Macrophage migration inhibitory factor is a well-known proinflammatory cytokine that is released during systemic stress response. Although MIF can affect erythrocyte production, the role of this cytokine in stress-induced erythropoiesis is completely unknown. To extend our previous findings showing that chronic psychological stress stimulates extramedullary erythropoiesis, here we examined whether MIF is involved in the control of stress-induced erythropoietic response. Adult male C57BL/6 wild-type (WT) and MIF-KO (knock-out) mice were subjected to 2-h daily restraint stress for either 7 or 14 consecutive days. The number of erythroid progenitors and CD71/Ter119 profile of erythroid precursors were analyzed in the bone marrow and spleen. Additionally, MIF protein expression was assessed in WT mice. Our results demonstrated that chronic restraint stress enhanced the number of both erythroid progenitors and precursors in the spleen. Stress-induced increase in the number of splenic late erythroid progenitors as well as in the percentage of CD71(+)Ter119(+)-double-positive precursors was significantly more pronounced in MIF-KO mice compared to WT animals. Furthermore, repeatedly stressed WT animals demonstrated an augmented MIF expression in the spleen. Unlike the spleen, the bone marrow of chronically stressed WT mice exhibited less prominent changes in erythropoietic stress response and no significant alteration in MIF expression. In addition, MIF deficiency did not influence the bone marrow erythropoiesis in stressed animals. These findings suggest that MIF regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions.
PB  - Springer, New York
T2  - Histochemistry & Cell Biology
T1  - Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis
EP  - 324
IS  - 3
SP  - 311
VL  - 146
DO  - 10.1007/s00418-016-1442-7
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Budeč, Mirela and Marković, Dragana and Gotić, Mirjana and Mitrović-Ajtić, Olivera and Mojsilović, Slavko and Stošić-Grujičić, Stanislava and Ivanov, Milan and Jovčić, Gordana and Čokić, Vladan",
year = "2016",
abstract = "Macrophage migration inhibitory factor is a well-known proinflammatory cytokine that is released during systemic stress response. Although MIF can affect erythrocyte production, the role of this cytokine in stress-induced erythropoiesis is completely unknown. To extend our previous findings showing that chronic psychological stress stimulates extramedullary erythropoiesis, here we examined whether MIF is involved in the control of stress-induced erythropoietic response. Adult male C57BL/6 wild-type (WT) and MIF-KO (knock-out) mice were subjected to 2-h daily restraint stress for either 7 or 14 consecutive days. The number of erythroid progenitors and CD71/Ter119 profile of erythroid precursors were analyzed in the bone marrow and spleen. Additionally, MIF protein expression was assessed in WT mice. Our results demonstrated that chronic restraint stress enhanced the number of both erythroid progenitors and precursors in the spleen. Stress-induced increase in the number of splenic late erythroid progenitors as well as in the percentage of CD71(+)Ter119(+)-double-positive precursors was significantly more pronounced in MIF-KO mice compared to WT animals. Furthermore, repeatedly stressed WT animals demonstrated an augmented MIF expression in the spleen. Unlike the spleen, the bone marrow of chronically stressed WT mice exhibited less prominent changes in erythropoietic stress response and no significant alteration in MIF expression. In addition, MIF deficiency did not influence the bone marrow erythropoiesis in stressed animals. These findings suggest that MIF regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions.",
publisher = "Springer, New York",
journal = "Histochemistry & Cell Biology",
title = "Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis",
pages = "324-311",
number = "3",
volume = "146",
doi = "10.1007/s00418-016-1442-7"
}

Vignjević-Petrinović, S., Budeč, M., Marković, D., Gotić, M., Mitrović-Ajtić, O., Mojsilović, S., Stošić-Grujičić, S., Ivanov, M., Jovčić, G.,& Čokić, V.. (2016). Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. in Histochemistry & Cell Biology
Springer, New York., 146(3), 311-324.
https://doi.org/10.1007/s00418-016-1442-7

Vignjević-Petrinović S, Budeč M, Marković D, Gotić M, Mitrović-Ajtić O, Mojsilović S, Stošić-Grujičić S, Ivanov M, Jovčić G, Čokić V. Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. in Histochemistry & Cell Biology. 2016;146(3):311-324.
doi:10.1007/s00418-016-1442-7 .

Vignjević-Petrinović, Sanja, Budeč, Mirela, Marković, Dragana, Gotić, Mirjana, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, Stošić-Grujičić, Stanislava, Ivanov, Milan, Jovčić, Gordana, Čokić, Vladan, "Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis" in Histochemistry & Cell Biology, 146, no. 3 (2016):311-324,
https://doi.org/10.1007/s00418-016-1442-7 . .

TY  - CONF
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Marković, Dragana
AU  - Mitrović, Olivera
AU  - Mojsilović, S.
AU  - Stošić-Grujičić, Stanislava
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/564
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress
EP  - 179
SP  - 179
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimi_564
ER  - 

@conference{
author = "Budeč, Mirela and Vignjević, Sanja and Marković, Dragana and Mitrović, Olivera and Mojsilović, S. and Stošić-Grujičić, Stanislava and Čokić, Vladan and Jovčić, Gordana",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress",
pages = "179-179",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_rimi_564"
}

Budeč, M., Vignjević, S., Marković, D., Mitrović, O., Mojsilović, S., Stošić-Grujičić, S., Čokić, V.,& Jovčić, G.. (2014). Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 179-179.
https://hdl.handle.net/21.15107/rcub_rimi_564

Budeč M, Vignjević S, Marković D, Mitrović O, Mojsilović S, Stošić-Grujičić S, Čokić V, Jovčić G. Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress. in Haematologica. 2014;99:179-179.
https://hdl.handle.net/21.15107/rcub_rimi_564 .

Budeč, Mirela, Vignjević, Sanja, Marković, Dragana, Mitrović, Olivera, Mojsilović, S., Stošić-Grujičić, Stanislava, Čokić, Vladan, Jovčić, Gordana, "Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress" in Haematologica, 99 (2014):179-179,
https://hdl.handle.net/21.15107/rcub_rimi_564 .

TY  - JOUR
AU  - Jovčić, Gordana
AU  - Ivanović, Z
AU  - Biljanovic-Paunović, L
AU  - Bugarski, Diana
AU  - Stošić-Grujičić, Stanislava
AU  - Milenković, P.
PY  - 1996
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/25
AB  - The multiple effects of interleukin-1 (IL-1) on hematopoietic cells are mainly documented in disturbed hematopoiesis, but its production and participation during constitutive hematopoiesis are still unproven, To assess the involvement of IL-1 in the regulation of steady-state hematopoiesis in vivo, we have investigated the consequences of IL-1 receptor blockade by recombinant human IL-1 receptor antagonist (rhIL-1Ra) in normal CBA/H mice treated with two i.p. injections of rhIL-1Ra (2 x 50 mu g/mouse) seventeen and two hours before sacrifice. The cellularity, the number of granulocyte-macrophage (CFU-GM), the number of erythroid (BFU-E) progenitor cells and the percentage of these cells in S phase of the cell cycle, as well as the morphologically recognizable cells in bone marrow were estimated, In peripheral blood, hematocrit, the number and differential count of nucleated cells, the number of erythrocytes and the percentage of reticulocytes were determined, IL-1Ra treatment significantly reduced the number of femoral CFU-GM and BFU-E, while all the other analyzed parameters were not different from the level obtained in control, non-treated animals. These findings show that a number of bone marrow IL-l-responsive cells were affected by the IL-1 receptor blockade, indicating that the expression of IL-1 receptors and endogenous IL-1 secretion occur as part of constitutive hematopoiesis.
PB  - John Libbey Eurotext Ltd, Montrouge
T2  - European Cytokine Network
T1  - In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice
EP  - 74
IS  - 1
SP  - 71
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_25
ER  - 

@article{
author = "Jovčić, Gordana and Ivanović, Z and Biljanovic-Paunović, L and Bugarski, Diana and Stošić-Grujičić, Stanislava and Milenković, P.",
year = "1996",
abstract = "The multiple effects of interleukin-1 (IL-1) on hematopoietic cells are mainly documented in disturbed hematopoiesis, but its production and participation during constitutive hematopoiesis are still unproven, To assess the involvement of IL-1 in the regulation of steady-state hematopoiesis in vivo, we have investigated the consequences of IL-1 receptor blockade by recombinant human IL-1 receptor antagonist (rhIL-1Ra) in normal CBA/H mice treated with two i.p. injections of rhIL-1Ra (2 x 50 mu g/mouse) seventeen and two hours before sacrifice. The cellularity, the number of granulocyte-macrophage (CFU-GM), the number of erythroid (BFU-E) progenitor cells and the percentage of these cells in S phase of the cell cycle, as well as the morphologically recognizable cells in bone marrow were estimated, In peripheral blood, hematocrit, the number and differential count of nucleated cells, the number of erythrocytes and the percentage of reticulocytes were determined, IL-1Ra treatment significantly reduced the number of femoral CFU-GM and BFU-E, while all the other analyzed parameters were not different from the level obtained in control, non-treated animals. These findings show that a number of bone marrow IL-l-responsive cells were affected by the IL-1 receptor blockade, indicating that the expression of IL-1 receptors and endogenous IL-1 secretion occur as part of constitutive hematopoiesis.",
publisher = "John Libbey Eurotext Ltd, Montrouge",
journal = "European Cytokine Network",
title = "In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice",
pages = "74-71",
number = "1",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_25"
}

Jovčić, G., Ivanović, Z., Biljanovic-Paunović, L., Bugarski, D., Stošić-Grujičić, S.,& Milenković, P.. (1996). In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice. in European Cytokine Network
John Libbey Eurotext Ltd, Montrouge., 7(1), 71-74.
https://hdl.handle.net/21.15107/rcub_rimi_25

Jovčić G, Ivanović Z, Biljanovic-Paunović L, Bugarski D, Stošić-Grujičić S, Milenković P. In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice. in European Cytokine Network. 1996;7(1):71-74.
https://hdl.handle.net/21.15107/rcub_rimi_25 .

Jovčić, Gordana, Ivanović, Z, Biljanovic-Paunović, L, Bugarski, Diana, Stošić-Grujičić, Stanislava, Milenković, P., "In vivo effects of interleukin-1 receptor antagonist on hematopoietic bone marrow progenitor cells in normal mice" in European Cytokine Network, 7, no. 1 (1996):71-74,
https://hdl.handle.net/21.15107/rcub_rimi_25 .

TY  - CONF
AU  - Milenković, P.
AU  - Ivanović, Z
AU  - Iovejc, G
AU  - Bugarski, Diana
AU  - Stošić-Grujičić, Stanislava
PY  - 1996
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/29
PB  - Elsevier Science Inc, New York
C3  - Experimental Hematology
T1  - Interleukin-1 receptor antagonist as an inhibitor of hemopoietic stem cell proliferation.
EP  - 36
IS  - 9
SP  - 36
VL  - 24
UR  - https://hdl.handle.net/21.15107/rcub_rimi_29
ER  - 

@conference{
author = "Milenković, P. and Ivanović, Z and Iovejc, G and Bugarski, Diana and Stošić-Grujičić, Stanislava",
year = "1996",
publisher = "Elsevier Science Inc, New York",
journal = "Experimental Hematology",
title = "Interleukin-1 receptor antagonist as an inhibitor of hemopoietic stem cell proliferation.",
pages = "36-36",
number = "9",
volume = "24",
url = "https://hdl.handle.net/21.15107/rcub_rimi_29"
}

Milenković, P., Ivanović, Z., Iovejc, G., Bugarski, D.,& Stošić-Grujičić, S.. (1996). Interleukin-1 receptor antagonist as an inhibitor of hemopoietic stem cell proliferation.. in Experimental Hematology
Elsevier Science Inc, New York., 24(9), 36-36.
https://hdl.handle.net/21.15107/rcub_rimi_29

Milenković P, Ivanović Z, Iovejc G, Bugarski D, Stošić-Grujičić S. Interleukin-1 receptor antagonist as an inhibitor of hemopoietic stem cell proliferation.. in Experimental Hematology. 1996;24(9):36-36.
https://hdl.handle.net/21.15107/rcub_rimi_29 .

Milenković, P., Ivanović, Z, Iovejc, G, Bugarski, Diana, Stošić-Grujičić, Stanislava, "Interleukin-1 receptor antagonist as an inhibitor of hemopoietic stem cell proliferation." in Experimental Hematology, 24, no. 9 (1996):36-36,
https://hdl.handle.net/21.15107/rcub_rimi_29 .