TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Željko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Milanović, Zorana
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/762
AB  - Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts
EP  - 437
IS  - 4
SP  - 429
VL  - 72
DO  - 10.1007/s10493-017-0170-6
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Željko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Milanović, Zorana and Tomanović, Snežana",
year = "2017",
abstract = "Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts",
pages = "437-429",
number = "4",
volume = "72",
doi = "10.1007/s10493-017-0170-6"
}

Mihaljica, D., Marković, D., Radulović, Ž., Mulenga, A., Ćakić, S., Sukara, R., Milanović, Z.,& Tomanović, S.. (2017). Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology
Springer, Dordrecht., 72(4), 429-437.
https://doi.org/10.1007/s10493-017-0170-6

Mihaljica D, Marković D, Radulović Ž, Mulenga A, Ćakić S, Sukara R, Milanović Z, Tomanović S. Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology. 2017;72(4):429-437.
doi:10.1007/s10493-017-0170-6 .

Mihaljica, Darko, Marković, Dragana, Radulović, Željko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Milanović, Zorana, Tomanović, Snežana, "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts" in Experimental & Applied Acarology, 72, no. 4 (2017):429-437,
https://doi.org/10.1007/s10493-017-0170-6 . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Obrenović, Sonja
AU  - Grujić-Milanović, Jelica
AU  - Arsić, Aleksandra
AU  - Milanović, Zorana
AU  - Savić, Olivera
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/770
AB  - Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers
EP  - 227
IS  - 2
SP  - 207
VL  - 67
DO  - 10.1515/acve-2017-0018
ER  - 

@article{
author = "Kovačić, Marijana and Marković, Dragana and Maslovarić, Irina and Obrenović, Sonja and Grujić-Milanović, Jelica and Arsić, Aleksandra and Milanović, Zorana and Savić, Olivera and Fratrić, Natalija and Ilić, Vesna",
year = "2017",
abstract = "Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers",
pages = "227-207",
number = "2",
volume = "67",
doi = "10.1515/acve-2017-0018"
}

Kovačić, M., Marković, D., Maslovarić, I., Obrenović, S., Grujić-Milanović, J., Arsić, A., Milanović, Z., Savić, O., Fratrić, N.,& Ilić, V.. (2017). Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 207-227.
https://doi.org/10.1515/acve-2017-0018

Kovačić M, Marković D, Maslovarić I, Obrenović S, Grujić-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd. 2017;67(2):207-227.
doi:10.1515/acve-2017-0018 .

Kovačić, Marijana, Marković, Dragana, Maslovarić, Irina, Obrenović, Sonja, Grujić-Milanović, Jelica, Arsić, Aleksandra, Milanović, Zorana, Savić, Olivera, Fratrić, Natalija, Ilić, Vesna, "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers" in Acta Veterinaria-Beograd, 67, no. 2 (2017):207-227,
https://doi.org/10.1515/acve-2017-0018 . .