TY  - JOUR
AU  - Sukara, Ratko
AU  - Juwaid, Salem
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Radojicić, Sonja
AU  - Hodzić, Adnan
AU  - Duscher, Gerhard Georg
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/929
AB  - Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia
EP  - 324
IS  - 3
SP  - 312
VL  - 69
DO  - 10.2478/acve-2019-0026
ER  - 

@article{
author = "Sukara, Ratko and Juwaid, Salem and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Veinović, Gorana and Radojicić, Sonja and Hodzić, Adnan and Duscher, Gerhard Georg and Tomanović, Snežana",
year = "2019",
abstract = "Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia",
pages = "324-312",
number = "3",
volume = "69",
doi = "10.2478/acve-2019-0026"
}

Sukara, R., Juwaid, S., Ćirović, D., Penezić, A., Mihaljica, D., Veinović, G., Radojicić, S., Hodzić, A., Duscher, G. G.,& Tomanović, S.. (2019). Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3), 312-324.
https://doi.org/10.2478/acve-2019-0026

Sukara R, Juwaid S, Ćirović D, Penezić A, Mihaljica D, Veinović G, Radojicić S, Hodzić A, Duscher GG, Tomanović S. Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd. 2019;69(3):312-324.
doi:10.2478/acve-2019-0026 .

Sukara, Ratko, Juwaid, Salem, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Veinović, Gorana, Radojicić, Sonja, Hodzić, Adnan, Duscher, Gerhard Georg, Tomanović, Snežana, "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia" in Acta Veterinaria-Beograd, 69, no. 3 (2019):312-324,
https://doi.org/10.2478/acve-2019-0026 . .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Veinović, Gorana
AU  - Cerar, T.
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ruzic-Sabljić, E.
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/934
AB  - Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.
PB  - Wiley, Hoboken
T2  - Medical & Veterinary Entomology
T1  - Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia
EP  - 520
IS  - 4
SP  - 512
VL  - 33
DO  - 10.1111/mve.12392
ER  - 

@article{
author = "Ćakić, Sanja and Veinović, Gorana and Cerar, T. and Mihaljica, Darko and Sukara, Ratko and Ruzic-Sabljić, E. and Tomanović, Snežana",
year = "2019",
abstract = "Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.",
publisher = "Wiley, Hoboken",
journal = "Medical & Veterinary Entomology",
title = "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia",
pages = "520-512",
number = "4",
volume = "33",
doi = "10.1111/mve.12392"
}

Ćakić, S., Veinović, G., Cerar, T., Mihaljica, D., Sukara, R., Ruzic-Sabljić, E.,& Tomanović, S.. (2019). Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology
Wiley, Hoboken., 33(4), 512-520.
https://doi.org/10.1111/mve.12392

Ćakić S, Veinović G, Cerar T, Mihaljica D, Sukara R, Ruzic-Sabljić E, Tomanović S. Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology. 2019;33(4):512-520.
doi:10.1111/mve.12392 .

Ćakić, Sanja, Veinović, Gorana, Cerar, T., Mihaljica, Darko, Sukara, Ratko, Ruzic-Sabljić, E., Tomanović, Snežana, "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia" in Medical & Veterinary Entomology, 33, no. 4 (2019):512-520,
https://doi.org/10.1111/mve.12392 . .

TY  - JOUR
AU  - Juwaid, Salem
AU  - Sukara, Ratko
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Kavallieratos, Nickolas G.
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/940
AB  - Tick-borne haematozoans cause severe diseases in domestic animals, and some of them have zoonotic potential. The results of previous studies in Europe point to the important role of foxes in natural endemic cycles of several tick-borne pathogens, including protozoa. The aim of the present research was to acquire information on the prevalence and distribution of tick-borne protozoan parasites among foxes in Serbia. Legally hunted foxes from 14 localities throughout Serbia were analysed. Spleen samples were collected from 129 animals and tested for the presence of Babesia spp. and Hepatozoon spp. by PCR. In total, 79/129 (61.2%) of the tested foxes were positive for H. canis, while the presence of two Babesia species was confirmed: B. vulpes (37/129, 28.7%) and B. canis (1/129, 0.8%). Co-infection with B. vulpes and H. canis was present in 26/129 (20.2%) foxes and one animal (1/129, 0.8%) was co-infected by B. canis and H. canis. The results of this study indicate the important role of foxes in the epizootiology of B. vulpes and H. canis in the Republic of Serbia and stress the need for further research to clarify all elements of the enzootic cycle of the detected pathogens, including other reservoirs, vectors, and transmission routes.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia
EP  - 80
IS  - 1
SP  - 70
VL  - 67
DO  - 10.1556/004.2019.008
ER  - 

@article{
author = "Juwaid, Salem and Sukara, Ratko and Penezić, Aleksandra and Mihaljica, Darko and Veinović, Gorana and Kavallieratos, Nickolas G. and Ćirović, Duško and Tomanović, Snežana",
year = "2019",
abstract = "Tick-borne haematozoans cause severe diseases in domestic animals, and some of them have zoonotic potential. The results of previous studies in Europe point to the important role of foxes in natural endemic cycles of several tick-borne pathogens, including protozoa. The aim of the present research was to acquire information on the prevalence and distribution of tick-borne protozoan parasites among foxes in Serbia. Legally hunted foxes from 14 localities throughout Serbia were analysed. Spleen samples were collected from 129 animals and tested for the presence of Babesia spp. and Hepatozoon spp. by PCR. In total, 79/129 (61.2%) of the tested foxes were positive for H. canis, while the presence of two Babesia species was confirmed: B. vulpes (37/129, 28.7%) and B. canis (1/129, 0.8%). Co-infection with B. vulpes and H. canis was present in 26/129 (20.2%) foxes and one animal (1/129, 0.8%) was co-infected by B. canis and H. canis. The results of this study indicate the important role of foxes in the epizootiology of B. vulpes and H. canis in the Republic of Serbia and stress the need for further research to clarify all elements of the enzootic cycle of the detected pathogens, including other reservoirs, vectors, and transmission routes.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia",
pages = "80-70",
number = "1",
volume = "67",
doi = "10.1556/004.2019.008"
}

Juwaid, S., Sukara, R., Penezić, A., Mihaljica, D., Veinović, G., Kavallieratos, N. G., Ćirović, D.,& Tomanović, S.. (2019). First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(1), 70-80.
https://doi.org/10.1556/004.2019.008

Juwaid S, Sukara R, Penezić A, Mihaljica D, Veinović G, Kavallieratos NG, Ćirović D, Tomanović S. First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia. in Acta Veterinaria Hungarica. 2019;67(1):70-80.
doi:10.1556/004.2019.008 .

Juwaid, Salem, Sukara, Ratko, Penezić, Aleksandra, Mihaljica, Darko, Veinović, Gorana, Kavallieratos, Nickolas G., Ćirović, Duško, Tomanović, Snežana, "First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia" in Acta Veterinaria Hungarica, 67, no. 1 (2019):70-80,
https://doi.org/10.1556/004.2019.008 . .

TY  - THES
AU  - Sukara, Ratko
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7280
UR  - http://nardus.mpn.gov.rs/handle/123456789/12114
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:21027/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=51824143
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1124
AB  - epizootiological and epidemiological role of the golden jackals (Canis aureus) as potential reservoirs for zoonotic vector-borne pathogens, for the first time in Serbia. Jackal spleens and collected ticks were tested by molecular methods (qPCR, conventional PCR, RFLP, sequencing) for the presence of vector-borne pathogens from the genera: Babesia spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp., Francisella spp., Bartonella spp., Leishmania spp., and species from Borrelia burgdorferi sensu lato complex and Coxiella burneti. A total of 216 jackals originating from 10 localities throughout the territory of the Republic of Serbia were analyzed. Beside that, 118 ticks collected from jackals were also tested. The collected ticks belonged to one of three species: Ixodes ricinus (54,2%), Dermacentor reticulatus (39,8%) i Haemaphysalis concinna (5,9%). The presence of DNA of Babesia canis with the prevalence of 4.2% (9/216), Anaplasma phagocytophilum (0.9%, 2/216) and Leishmania spp. (6.9%, 15/216) was confirmed by molecular methods in the spleen of the tested jackals. In ticks collected from jackals, the presence of DNA from two Babesia spp. (Babesia canis, Babesia microti), Anaplasma phagocytophilum and three species from the Borrelia burgdorferi s.l. complex (Borrelia garinii, Borrelia lusitaniae, Borrelia valaisiana) was confirmed...
AB  - Istraživanjem sprovedenim u okviru ove doktorske disertacije, po prvi put u Srbiji, analiziran je epizootiološko-epidemiološki značaj šakala (Canis aureus) kao potencijalnih rezervoara za uzročnike zoonoza koje se prenose vektorima. Molekularnim metodama (qPCR, konvencionalni PCR, RFLP, sekvenciranje) u slezinama šakala i krpeljima sakupljenim sa šakala, dokazivano je prisustvo vektorima prenosivih patogena iz rodova: Babesia spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp., Francisella spp., Bartonella spp., Leishmania spp., i vrsta u okviru Borrelia burgdorferi sensu lato kompleksa i Coxiella burneti. Ukupno je analizirano 216 šakala poreklom sa 10 lokaliteta širom teritorije Republike Srbije. Na prisustvo patogena ispitano je i 118 sakupljenih krpelja koji su pripadali jednoj od tri vrste: Ixodes ricinus (54,2%), Dermacentor reticulatus (39,8%) i Haemaphysalis concinna (5,9%). Molekularnim metodama u slezinama šakala potvrđeno je prisustvo DNK Babesia canis sa prevalencijom od 4,2% (9/216), Anaplasma phagocytophilum (0,9%, 2/216) i Leishmania spp. (6,9%, 15/216). U krpeljima sakupljenim sa šakala potvrđeno je prisustvo DNK dve vrste Babesia (Babesia canis, Babesia microti), Anaplasma phagocytophilum i tri vrste Borrelia burgdorferi s.l. kompleksa (Borrelia garinii, Borrelia lusitaniae, Borrelia valaisiana)...
PB  - Univerzitet u Beogradu, Fakultet veterinarske medicine
T1  - Еpizootiological-epidemiological importance of the golden jackal (Canis aureus) in the maintenance of vektor-borne zoonoses in the territory of the Republic of Serbia
T1  - Epizootiološko-epidemiološki značaj zlatnog šakala (Canis aureus) u održavanju vektorima prenosivih zoonoza na teritoriji Republike Srbije
UR  - https://hdl.handle.net/21.15107/rcub_nardus_12114
ER  - 

@phdthesis{
author = "Sukara, Ratko",
year = "2019",
abstract = "epizootiological and epidemiological role of the golden jackals (Canis aureus) as potential reservoirs for zoonotic vector-borne pathogens, for the first time in Serbia. Jackal spleens and collected ticks were tested by molecular methods (qPCR, conventional PCR, RFLP, sequencing) for the presence of vector-borne pathogens from the genera: Babesia spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp., Francisella spp., Bartonella spp., Leishmania spp., and species from Borrelia burgdorferi sensu lato complex and Coxiella burneti. A total of 216 jackals originating from 10 localities throughout the territory of the Republic of Serbia were analyzed. Beside that, 118 ticks collected from jackals were also tested. The collected ticks belonged to one of three species: Ixodes ricinus (54,2%), Dermacentor reticulatus (39,8%) i Haemaphysalis concinna (5,9%). The presence of DNA of Babesia canis with the prevalence of 4.2% (9/216), Anaplasma phagocytophilum (0.9%, 2/216) and Leishmania spp. (6.9%, 15/216) was confirmed by molecular methods in the spleen of the tested jackals. In ticks collected from jackals, the presence of DNA from two Babesia spp. (Babesia canis, Babesia microti), Anaplasma phagocytophilum and three species from the Borrelia burgdorferi s.l. complex (Borrelia garinii, Borrelia lusitaniae, Borrelia valaisiana) was confirmed..., Istraživanjem sprovedenim u okviru ove doktorske disertacije, po prvi put u Srbiji, analiziran je epizootiološko-epidemiološki značaj šakala (Canis aureus) kao potencijalnih rezervoara za uzročnike zoonoza koje se prenose vektorima. Molekularnim metodama (qPCR, konvencionalni PCR, RFLP, sekvenciranje) u slezinama šakala i krpeljima sakupljenim sa šakala, dokazivano je prisustvo vektorima prenosivih patogena iz rodova: Babesia spp., Rickettsia spp., Anaplasma spp., Ehrlichia spp., Francisella spp., Bartonella spp., Leishmania spp., i vrsta u okviru Borrelia burgdorferi sensu lato kompleksa i Coxiella burneti. Ukupno je analizirano 216 šakala poreklom sa 10 lokaliteta širom teritorije Republike Srbije. Na prisustvo patogena ispitano je i 118 sakupljenih krpelja koji su pripadali jednoj od tri vrste: Ixodes ricinus (54,2%), Dermacentor reticulatus (39,8%) i Haemaphysalis concinna (5,9%). Molekularnim metodama u slezinama šakala potvrđeno je prisustvo DNK Babesia canis sa prevalencijom od 4,2% (9/216), Anaplasma phagocytophilum (0,9%, 2/216) i Leishmania spp. (6,9%, 15/216). U krpeljima sakupljenim sa šakala potvrđeno je prisustvo DNK dve vrste Babesia (Babesia canis, Babesia microti), Anaplasma phagocytophilum i tri vrste Borrelia burgdorferi s.l. kompleksa (Borrelia garinii, Borrelia lusitaniae, Borrelia valaisiana)...",
publisher = "Univerzitet u Beogradu, Fakultet veterinarske medicine",
title = "Еpizootiological-epidemiological importance of the golden jackal (Canis aureus) in the maintenance of vektor-borne zoonoses in the territory of the Republic of Serbia, Epizootiološko-epidemiološki značaj zlatnog šakala (Canis aureus) u održavanju vektorima prenosivih zoonoza na teritoriji Republike Srbije",
url = "https://hdl.handle.net/21.15107/rcub_nardus_12114"
}

Sukara, R.. (2019). Еpizootiological-epidemiological importance of the golden jackal (Canis aureus) in the maintenance of vektor-borne zoonoses in the territory of the Republic of Serbia. 
Univerzitet u Beogradu, Fakultet veterinarske medicine..
https://hdl.handle.net/21.15107/rcub_nardus_12114

Sukara R. Еpizootiological-epidemiological importance of the golden jackal (Canis aureus) in the maintenance of vektor-borne zoonoses in the territory of the Republic of Serbia. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_12114 .

Sukara, Ratko, "Еpizootiological-epidemiological importance of the golden jackal (Canis aureus) in the maintenance of vektor-borne zoonoses in the territory of the Republic of Serbia" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_12114 .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Chochlakis, Dimosthenis
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Ćakić, Sanja
AU  - Valcić, Miroslav
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
AU  - Tomanović, Snežana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/893
AB  - The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia. Ticks, when present, were removed, and after necropsy, spleen samples were collected from each animal. All tick and spleen samples were tested for the DNA of bacterial and protozoan tick-borne pathogens (Borrelia species, Bartonella species, Rickettsia species, Anaplasma species, Coxiella burnetii, Francisella species and Babesia species) by multiplex real-time PCR, conventional PCR and sequencing analyses. The DNA of Babesia canis was detected in nine out of 216 (4.2%) spleen samples, and two samples (0.9%) tested positive for Anaplasma phagocytophilum. In 118 ticks collected from jackals, the DNA of two Babesia species (Ba. canis and Ba. microti), three Borrelia species (Bo. garinii, Bo. valaisiana, and Bo. lusitaniae) and A. marginale was detected. From the aspect of public health surveillance, the potential role of the golden jackal in the maintenance of vector-borne zoonotic pathogens in Serbia must be considered, and further eco-epidemiological studies should be performed to determine the precise role of this animal species in zoonotic disease transmission cycles.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks & Tick-Borne Diseases
T1  - Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia
EP  - 1097
IS  - 5
SP  - 1090
VL  - 9
DO  - 10.1016/j.ttbdis.2018.04.003
ER  - 

@article{
author = "Sukara, Ratko and Chochlakis, Dimosthenis and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Ćakić, Sanja and Valcić, Miroslav and Tselentis, Yannis and Psaroulaki, Anna and Tomanović, Snežana",
year = "2018",
abstract = "The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia. Ticks, when present, were removed, and after necropsy, spleen samples were collected from each animal. All tick and spleen samples were tested for the DNA of bacterial and protozoan tick-borne pathogens (Borrelia species, Bartonella species, Rickettsia species, Anaplasma species, Coxiella burnetii, Francisella species and Babesia species) by multiplex real-time PCR, conventional PCR and sequencing analyses. The DNA of Babesia canis was detected in nine out of 216 (4.2%) spleen samples, and two samples (0.9%) tested positive for Anaplasma phagocytophilum. In 118 ticks collected from jackals, the DNA of two Babesia species (Ba. canis and Ba. microti), three Borrelia species (Bo. garinii, Bo. valaisiana, and Bo. lusitaniae) and A. marginale was detected. From the aspect of public health surveillance, the potential role of the golden jackal in the maintenance of vector-borne zoonotic pathogens in Serbia must be considered, and further eco-epidemiological studies should be performed to determine the precise role of this animal species in zoonotic disease transmission cycles.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks & Tick-Borne Diseases",
title = "Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia",
pages = "1097-1090",
number = "5",
volume = "9",
doi = "10.1016/j.ttbdis.2018.04.003"
}

Sukara, R., Chochlakis, D., Ćirović, D., Penezić, A., Mihaljica, D., Ćakić, S., Valcić, M., Tselentis, Y., Psaroulaki, A.,& Tomanović, S.. (2018). Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 9(5), 1090-1097.
https://doi.org/10.1016/j.ttbdis.2018.04.003

Sukara R, Chochlakis D, Ćirović D, Penezić A, Mihaljica D, Ćakić S, Valcić M, Tselentis Y, Psaroulaki A, Tomanović S. Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia. in Ticks & Tick-Borne Diseases. 2018;9(5):1090-1097.
doi:10.1016/j.ttbdis.2018.04.003 .

Sukara, Ratko, Chochlakis, Dimosthenis, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Ćakić, Sanja, Valcić, Miroslav, Tselentis, Yannis, Psaroulaki, Anna, Tomanović, Snežana, "Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia" in Ticks & Tick-Borne Diseases, 9, no. 5 (2018):1090-1097,
https://doi.org/10.1016/j.ttbdis.2018.04.003 . .

TY  - JOUR
AU  - Burazerović, Jelena
AU  - Orlova, M.
AU  - Obradović, M.
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/889
AB  - Bats are hosts to a number of ectoparasites-acarines (ticks, chiggers, other mites), bat flies, and fleas. Bat ectoparasites might have significant ecological and public health importance as they may be potential vectors of zoonotic agents. It is important to identify their distribution, diversity, and host-parasite associations. Bat ectoparasites in the central Balkans have been largely understudied. The present research was conducted in 45 localities at the territory of Bosnia and Herzegovina, former Yugoslav Republic of Macedonia, Montenegro, and Serbia. In total, 1,143 individuals of 18 species of bats have been examined for the presence and abundance of ectoparasite species during 3 yr of research. In total, 21 ectoparasite species have been identified: three species of ticks, seven species of mites (including one species of chigger), eight species of bat flies, and three species of fleas. In total, 80 host-parasite associations have been identified. The largest number of ectoparasites parasitized primarily only one host species. The highest total number of hosts was identified for ectoparasite species Ixodes vespertilionis Koch, Nycteribia schmidlii Schiner, and Spinturnix myoti Kolenati. The spinturnicid mite Spinturnix psi Kolenati was the most abundant ectoparasite species and together with Penicilidia dufouri Westwood the most widely distributed species of bat ectoparasite, being present at 21 localities in the central Balkans. The presented data include the first systematic records of patterns of prevalence, mean intensity, mean abundance, and host specificity for bat ectoparasites in the central Balkans.
PB  - Oxford Univ Press Inc, Cary
T2  - Journal of Medical Entomology
T1  - Patterns of Abundance and Host Specificity of Bat Ectoparasites in the Central Balkans
EP  - 28
IS  - 1
SP  - 20
VL  - 55
DO  - 10.1093/jme/tjx189
ER  - 

@article{
author = "Burazerović, Jelena and Orlova, M. and Obradović, M. and Ćirović, Duško and Tomanović, Snežana",
year = "2018",
abstract = "Bats are hosts to a number of ectoparasites-acarines (ticks, chiggers, other mites), bat flies, and fleas. Bat ectoparasites might have significant ecological and public health importance as they may be potential vectors of zoonotic agents. It is important to identify their distribution, diversity, and host-parasite associations. Bat ectoparasites in the central Balkans have been largely understudied. The present research was conducted in 45 localities at the territory of Bosnia and Herzegovina, former Yugoslav Republic of Macedonia, Montenegro, and Serbia. In total, 1,143 individuals of 18 species of bats have been examined for the presence and abundance of ectoparasite species during 3 yr of research. In total, 21 ectoparasite species have been identified: three species of ticks, seven species of mites (including one species of chigger), eight species of bat flies, and three species of fleas. In total, 80 host-parasite associations have been identified. The largest number of ectoparasites parasitized primarily only one host species. The highest total number of hosts was identified for ectoparasite species Ixodes vespertilionis Koch, Nycteribia schmidlii Schiner, and Spinturnix myoti Kolenati. The spinturnicid mite Spinturnix psi Kolenati was the most abundant ectoparasite species and together with Penicilidia dufouri Westwood the most widely distributed species of bat ectoparasite, being present at 21 localities in the central Balkans. The presented data include the first systematic records of patterns of prevalence, mean intensity, mean abundance, and host specificity for bat ectoparasites in the central Balkans.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journal of Medical Entomology",
title = "Patterns of Abundance and Host Specificity of Bat Ectoparasites in the Central Balkans",
pages = "28-20",
number = "1",
volume = "55",
doi = "10.1093/jme/tjx189"
}

Burazerović, J., Orlova, M., Obradović, M., Ćirović, D.,& Tomanović, S.. (2018). Patterns of Abundance and Host Specificity of Bat Ectoparasites in the Central Balkans. in Journal of Medical Entomology
Oxford Univ Press Inc, Cary., 55(1), 20-28.
https://doi.org/10.1093/jme/tjx189

Burazerović J, Orlova M, Obradović M, Ćirović D, Tomanović S. Patterns of Abundance and Host Specificity of Bat Ectoparasites in the Central Balkans. in Journal of Medical Entomology. 2018;55(1):20-28.
doi:10.1093/jme/tjx189 .

Burazerović, Jelena, Orlova, M., Obradović, M., Ćirović, Duško, Tomanović, Snežana, "Patterns of Abundance and Host Specificity of Bat Ectoparasites in the Central Balkans" in Journal of Medical Entomology, 55, no. 1 (2018):20-28,
https://doi.org/10.1093/jme/tjx189 . .

TY  - JOUR
AU  - Hornok, Sandor
AU  - Sandor, Attila D.
AU  - Beck, Relja
AU  - Farkas, Robert
AU  - Beati, Lorenza
AU  - Kontschan, Jeno
AU  - Takacs, Nora
AU  - Foldavari, Gabor
AU  - Silaghi, Cornelia
AU  - Meyer-Kayser, Elisabeth
AU  - Hodzić, Adnan
AU  - Tomanović, Snežana
AU  - Abdullah, Swaid
AU  - Wall, Richard
AU  - Estrada-Pena, Agustin
AU  - Duscher, Georg Gerhard
AU  - Plantard, Olivier
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/783
AB  - Background: In Europe, hard ticks of the subgenus Pholeoixodes (Ixodidae: Ixodes) are usually associated with burrow-dwelling mammals and terrestrial birds. Reports of Pholeoixodes spp. from carnivores are frequently contradictory, and their identification is not based on key diagnostic characters. Therefore, the aims of the present study were to identify ticks collected from dogs, foxes and badgers in several European countries, and to reassess their systematic status with molecular analyses using two mitochondrial markers. Results: Between 2003 and 2017, 144 Pholeoixodes spp. ticks were collected in nine European countries. From accurate descriptions and comparison with type-materials, a simple illustrated identification key was compiled for adult females, by focusing on the shape of the anterior surface of basis capituli. Based on this key, 71 female ticks were identified as I. canisuga, 21 as I. kaiseri and 21 as I. hexagonus. DNA was extracted from these 113 female ticks, and from further 31 specimens. Fragments of two mitochondrial genes, cox1 (cytochrome c oxidase subunit 1) and 16S rRNA, were amplified and sequenced. Ixodes kaiseri had nine unique cox1 haplotypes, which showed 99.2-100% sequence identity, whereas I. canisuga and I. hexagonus had eleven and five cox1 haplotypes, respectively, with 99.5-100% sequence identity. The distribution of cox1 haplotypes reflected a geographical pattern. Pholeoixodes spp. ticks had fewer 16S rRNA haplotypes, with a lower degree of intraspecific divergence (99.5-100% sequence identity) and no geographical clustering. Phylogenetic analyses were in agreement with morphology: I. kaiseri and I. hexagonus (with the similar shape of the anterior surface of basis capituli) were genetically more closely related to each other than to I. canisuga. Phylogenetic analyses also showed that the subgenus Eschatocephalus (bat ticks) clustered within the subgenus Pholeoixodes. Conclusions: A simple, illustrated identification key is provided for female Pholeoixodes ticks of carnivores (including I. hexagonus and I. rugicollis) to prevent future misidentification of these species. It is also shown that I. kaiseri is more widespread in Europe than previously thought. Phylogenetic analyses suggest that the subgenus Pholeoixodes is not monophyletic: either the subgenus Eschatocephalus should be included in Pholeoixodes, or the latter subgenus should be divided, which is a task for future studies.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females
VL  - 10
DO  - 10.1186/s13071-017-2424-x
ER  - 

@article{
author = "Hornok, Sandor and Sandor, Attila D. and Beck, Relja and Farkas, Robert and Beati, Lorenza and Kontschan, Jeno and Takacs, Nora and Foldavari, Gabor and Silaghi, Cornelia and Meyer-Kayser, Elisabeth and Hodzić, Adnan and Tomanović, Snežana and Abdullah, Swaid and Wall, Richard and Estrada-Pena, Agustin and Duscher, Georg Gerhard and Plantard, Olivier",
year = "2017",
abstract = "Background: In Europe, hard ticks of the subgenus Pholeoixodes (Ixodidae: Ixodes) are usually associated with burrow-dwelling mammals and terrestrial birds. Reports of Pholeoixodes spp. from carnivores are frequently contradictory, and their identification is not based on key diagnostic characters. Therefore, the aims of the present study were to identify ticks collected from dogs, foxes and badgers in several European countries, and to reassess their systematic status with molecular analyses using two mitochondrial markers. Results: Between 2003 and 2017, 144 Pholeoixodes spp. ticks were collected in nine European countries. From accurate descriptions and comparison with type-materials, a simple illustrated identification key was compiled for adult females, by focusing on the shape of the anterior surface of basis capituli. Based on this key, 71 female ticks were identified as I. canisuga, 21 as I. kaiseri and 21 as I. hexagonus. DNA was extracted from these 113 female ticks, and from further 31 specimens. Fragments of two mitochondrial genes, cox1 (cytochrome c oxidase subunit 1) and 16S rRNA, were amplified and sequenced. Ixodes kaiseri had nine unique cox1 haplotypes, which showed 99.2-100% sequence identity, whereas I. canisuga and I. hexagonus had eleven and five cox1 haplotypes, respectively, with 99.5-100% sequence identity. The distribution of cox1 haplotypes reflected a geographical pattern. Pholeoixodes spp. ticks had fewer 16S rRNA haplotypes, with a lower degree of intraspecific divergence (99.5-100% sequence identity) and no geographical clustering. Phylogenetic analyses were in agreement with morphology: I. kaiseri and I. hexagonus (with the similar shape of the anterior surface of basis capituli) were genetically more closely related to each other than to I. canisuga. Phylogenetic analyses also showed that the subgenus Eschatocephalus (bat ticks) clustered within the subgenus Pholeoixodes. Conclusions: A simple, illustrated identification key is provided for female Pholeoixodes ticks of carnivores (including I. hexagonus and I. rugicollis) to prevent future misidentification of these species. It is also shown that I. kaiseri is more widespread in Europe than previously thought. Phylogenetic analyses suggest that the subgenus Pholeoixodes is not monophyletic: either the subgenus Eschatocephalus should be included in Pholeoixodes, or the latter subgenus should be divided, which is a task for future studies.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females",
volume = "10",
doi = "10.1186/s13071-017-2424-x"
}

Hornok, S., Sandor, A. D., Beck, R., Farkas, R., Beati, L., Kontschan, J., Takacs, N., Foldavari, G., Silaghi, C., Meyer-Kayser, E., Hodzić, A., Tomanović, S., Abdullah, S., Wall, R., Estrada-Pena, A., Duscher, G. G.,& Plantard, O.. (2017). Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females. in Parasites & Vectors
BMC, London., 10.
https://doi.org/10.1186/s13071-017-2424-x

Hornok S, Sandor AD, Beck R, Farkas R, Beati L, Kontschan J, Takacs N, Foldavari G, Silaghi C, Meyer-Kayser E, Hodzić A, Tomanović S, Abdullah S, Wall R, Estrada-Pena A, Duscher GG, Plantard O. Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females. in Parasites & Vectors. 2017;10.
doi:10.1186/s13071-017-2424-x .

Hornok, Sandor, Sandor, Attila D., Beck, Relja, Farkas, Robert, Beati, Lorenza, Kontschan, Jeno, Takacs, Nora, Foldavari, Gabor, Silaghi, Cornelia, Meyer-Kayser, Elisabeth, Hodzić, Adnan, Tomanović, Snežana, Abdullah, Swaid, Wall, Richard, Estrada-Pena, Agustin, Duscher, Georg Gerhard, Plantard, Olivier, "Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females" in Parasites & Vectors, 10 (2017),
https://doi.org/10.1186/s13071-017-2424-x . .

TY  - JOUR
AU  - Hornok, Sandor
AU  - Sandor, Attila D.
AU  - Tomanović, Snežana
AU  - Beck, Relja
AU  - D'Amico, Gianluca
AU  - Kontschan, Jeno
AU  - Takacs, Nora
AU  - Gorfol, Tamas
AU  - Bendjeddou, Mohammed Lamine
AU  - Foldvari, Gabor
AU  - Farkas, Robert
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/802
AB  - Background: Rhipicephalus sanguineus belongs to a complex of hard tick species with high veterinary-medical significance. Recently, new phylogenetic units have been discovered within R. sanguineus, which therefore needs taxonomic revision. The present study was initiated to provide new information on the phylogeography of relevant haplotypes from less studied regions of Europe and Africa. With this aim, molecular-phylogenetic analyses of two mitochondrial markers were performed on 50 ticks collected in Hungary, the Balkans, countries along the Mediterranean Sea, Kenya and Ivory Coast. Results: In the "temperate lineage" of R. sanguineus, based on cytochrome c oxidase subunit 1 (cox1) and 16S rRNA genes, Rhipicephalus sp. I was only found in the eastern part of the Mediterranean Basin (with relatively homogenous haplotypes), whereas Rhipicephalus sp. II occurred in the middle-to-western part of this region (with phylogenetically dichotomous haplotypes). Ticks identified as R. leporis (based on morphology and cox1 gene) were found in Kenya and Ivory Coast. These clustered phylogenetically within R. sanguineus (s.l.) ("tropical lineage"). Conclusions: In the Mediterranean Basin two mitochondrial lineages of R. sanguineus, i. e. Rhipicephalus sp. I and Rhipicephalus sp. II exist, which show different geographical distribution. Therefore, data from this study confirm limited gene flow between Rhipicephalus sp. I and Rhipicephalus sp. II, but more evidence (analyses of nuclear markers, extensive morphological and biological comparison etc.) are necessary to infer if they belong to different species or not. The phylogenetic relationships of eastern and western African ticks, which align with R. leporis, need to be studied further within R. sanguineus (s.l.) ("tropical lineage").
PB  - Biomed Central Ltd, London
T2  - Parasites & Vectors
T1  - East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin
VL  - 10
DO  - 10.1186/s13071-017-1985-z
ER  - 

@article{
author = "Hornok, Sandor and Sandor, Attila D. and Tomanović, Snežana and Beck, Relja and D'Amico, Gianluca and Kontschan, Jeno and Takacs, Nora and Gorfol, Tamas and Bendjeddou, Mohammed Lamine and Foldvari, Gabor and Farkas, Robert",
year = "2017",
abstract = "Background: Rhipicephalus sanguineus belongs to a complex of hard tick species with high veterinary-medical significance. Recently, new phylogenetic units have been discovered within R. sanguineus, which therefore needs taxonomic revision. The present study was initiated to provide new information on the phylogeography of relevant haplotypes from less studied regions of Europe and Africa. With this aim, molecular-phylogenetic analyses of two mitochondrial markers were performed on 50 ticks collected in Hungary, the Balkans, countries along the Mediterranean Sea, Kenya and Ivory Coast. Results: In the "temperate lineage" of R. sanguineus, based on cytochrome c oxidase subunit 1 (cox1) and 16S rRNA genes, Rhipicephalus sp. I was only found in the eastern part of the Mediterranean Basin (with relatively homogenous haplotypes), whereas Rhipicephalus sp. II occurred in the middle-to-western part of this region (with phylogenetically dichotomous haplotypes). Ticks identified as R. leporis (based on morphology and cox1 gene) were found in Kenya and Ivory Coast. These clustered phylogenetically within R. sanguineus (s.l.) ("tropical lineage"). Conclusions: In the Mediterranean Basin two mitochondrial lineages of R. sanguineus, i. e. Rhipicephalus sp. I and Rhipicephalus sp. II exist, which show different geographical distribution. Therefore, data from this study confirm limited gene flow between Rhipicephalus sp. I and Rhipicephalus sp. II, but more evidence (analyses of nuclear markers, extensive morphological and biological comparison etc.) are necessary to infer if they belong to different species or not. The phylogenetic relationships of eastern and western African ticks, which align with R. leporis, need to be studied further within R. sanguineus (s.l.) ("tropical lineage").",
publisher = "Biomed Central Ltd, London",
journal = "Parasites & Vectors",
title = "East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin",
volume = "10",
doi = "10.1186/s13071-017-1985-z"
}

Hornok, S., Sandor, A. D., Tomanović, S., Beck, R., D'Amico, G., Kontschan, J., Takacs, N., Gorfol, T., Bendjeddou, M. L., Foldvari, G.,& Farkas, R.. (2017). East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin. in Parasites & Vectors
Biomed Central Ltd, London., 10.
https://doi.org/10.1186/s13071-017-1985-z

Hornok S, Sandor AD, Tomanović S, Beck R, D'Amico G, Kontschan J, Takacs N, Gorfol T, Bendjeddou ML, Foldvari G, Farkas R. East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin. in Parasites & Vectors. 2017;10.
doi:10.1186/s13071-017-1985-z .

Hornok, Sandor, Sandor, Attila D., Tomanović, Snežana, Beck, Relja, D'Amico, Gianluca, Kontschan, Jeno, Takacs, Nora, Gorfol, Tamas, Bendjeddou, Mohammed Lamine, Foldvari, Gabor, Farkas, Robert, "East and west separation of Rhipicephalus sanguineus mitochondrial lineages in the Mediterranean Basin" in Parasites & Vectors, 10 (2017),
https://doi.org/10.1186/s13071-017-1985-z . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Željko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Milanović, Zorana
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/762
AB  - Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts
EP  - 437
IS  - 4
SP  - 429
VL  - 72
DO  - 10.1007/s10493-017-0170-6
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Željko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Milanović, Zorana and Tomanović, Snežana",
year = "2017",
abstract = "Due to the recorded spreading of ticks in past years, a higher incidence of tick-borne diseases (TBDs) can be expected in the future in endemic areas, but can also pose an emerging public health concern in areas where they have not yet been recognized. Assessment of the exposure of vulnerable hosts to ticks would be a very helpful tool for TBD epidemiological studies, as well as for their proper managing. To confirm previous tick bites, the method of choice is detection of antibodies in host serum as markers developed against injected tick saliva proteins during feeding. We recently showed that the recombinant form of Ixodes ricinus AV422 saliva protein (rIrAV422) can serve for detection of markers in experimentally infested rats. Here we examine whether it can be used in the same manner in naturally exposed hosts. We chose hunting dogs as good sentinel animals. The study group consisted of 15 dogs that varied in breed, age, sex, previous tick infestation history and repellent treatment. Western blot analysis with rIrAV422 as an antigen confirmed the presence of tick bite markers in all analysed dogs. For some of the dogs, their previous tick infestation history was unclear, which emphasizes the usefulness of rIrAV422 for revealing it. Since hunting dogs are naturally infested with different ticks, the potential of rIrAV422 in assessment of general exposure to ticks is highlighted. Use of rIrAV422 can also be helpful in veterinary practice and research as a tool for validation of the efficiency of tick repellent products.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts",
pages = "437-429",
number = "4",
volume = "72",
doi = "10.1007/s10493-017-0170-6"
}

Mihaljica, D., Marković, D., Radulović, Ž., Mulenga, A., Ćakić, S., Sukara, R., Milanović, Z.,& Tomanović, S.. (2017). Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology
Springer, Dordrecht., 72(4), 429-437.
https://doi.org/10.1007/s10493-017-0170-6

Mihaljica D, Marković D, Radulović Ž, Mulenga A, Ćakić S, Sukara R, Milanović Z, Tomanović S. Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts. in Experimental & Applied Acarology. 2017;72(4):429-437.
doi:10.1007/s10493-017-0170-6 .

Mihaljica, Darko, Marković, Dragana, Radulović, Željko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Milanović, Zorana, Tomanović, Snežana, "Assessment of using recombinant Ixodes ricinus AV422 saliva protein for confirmation of tick bites in hunting dogs as naturally infested hosts" in Experimental & Applied Acarology, 72, no. 4 (2017):429-437,
https://doi.org/10.1007/s10493-017-0170-6 . .

TY  - JOUR
AU  - Pajnik, Jelena
AU  - Stamenić, Marko
AU  - Radetić, Maja
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Mihaljica, Darko
AU  - Zizović, Irena
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/766
AB  - This study discusses a possibility of cotton fabric impregnation with pyrethrum extract as a tick repellent in supercritical carbon dioxide. A solubility of pyrethrum extract in supercritical carbon dioxide has been determined at 35 and 40 degrees C and pressures ranging from 8 to 20 MPa. The highest solubility of 48.69 kg/m(3) was obtained at temperature of 35 degrees C and pressure of 20 MPa. Density -based equations of Chrastil, Adachi-Lu and del Valle-Aguilera were employed to correlate the experimental data. Based on the solubility data, conditions for the impregnation of cotton fabric were selected. Conditions at 40 degrees C and 8 MPa provided targeted quantities of the impregnated extract of 0.5% and 1% after 1 h and 2 h of the impregnation, respectively. The presence of pyrethrins on the cotton fabric's surface was confirmed by FTIR analysis. Repellent activity of both impregnated cotton fabrics (pyrethrum extract contents of 0.5% and 1%) was proven against ticks.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide
EP  - 72
SP  - 66
VL  - 128
DO  - 10.1016/j.supflu.2017.05.006
ER  - 

@article{
author = "Pajnik, Jelena and Stamenić, Marko and Radetić, Maja and Tomanović, Snežana and Sukara, Ratko and Mihaljica, Darko and Zizović, Irena",
year = "2017",
abstract = "This study discusses a possibility of cotton fabric impregnation with pyrethrum extract as a tick repellent in supercritical carbon dioxide. A solubility of pyrethrum extract in supercritical carbon dioxide has been determined at 35 and 40 degrees C and pressures ranging from 8 to 20 MPa. The highest solubility of 48.69 kg/m(3) was obtained at temperature of 35 degrees C and pressure of 20 MPa. Density -based equations of Chrastil, Adachi-Lu and del Valle-Aguilera were employed to correlate the experimental data. Based on the solubility data, conditions for the impregnation of cotton fabric were selected. Conditions at 40 degrees C and 8 MPa provided targeted quantities of the impregnated extract of 0.5% and 1% after 1 h and 2 h of the impregnation, respectively. The presence of pyrethrins on the cotton fabric's surface was confirmed by FTIR analysis. Repellent activity of both impregnated cotton fabrics (pyrethrum extract contents of 0.5% and 1%) was proven against ticks.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide",
pages = "72-66",
volume = "128",
doi = "10.1016/j.supflu.2017.05.006"
}

Pajnik, J., Stamenić, M., Radetić, M., Tomanović, S., Sukara, R., Mihaljica, D.,& Zizović, I.. (2017). Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide. in Journal of Supercritical Fluids
Elsevier Science Bv, Amsterdam., 128, 66-72.
https://doi.org/10.1016/j.supflu.2017.05.006

Pajnik J, Stamenić M, Radetić M, Tomanović S, Sukara R, Mihaljica D, Zizović I. Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide. in Journal of Supercritical Fluids. 2017;128:66-72.
doi:10.1016/j.supflu.2017.05.006 .

Pajnik, Jelena, Stamenić, Marko, Radetić, Maja, Tomanović, Snežana, Sukara, Ratko, Mihaljica, Darko, Zizović, Irena, "Impregnation of cotton fabric with pyrethrum extract in supercritical carbon dioxide" in Journal of Supercritical Fluids, 128 (2017):66-72,
https://doi.org/10.1016/j.supflu.2017.05.006 . .

TY  - JOUR
AU  - Radulović, Željko
AU  - Mihaljica, Darko
AU  - Ćosić, Nada
AU  - Penezić, Aleksandra
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/777
AB  - Specimens of the endangered species Spermophilus citellus (European ground squirrel) from populations inhabiting 12 localities in Vojvodina (Serbia) were inspected for the presence of attached hard ticks. A total of 1009 ticks were found on 151 infested animals. With respect to the level of infestation, over 60% of the squirrels were found with five or less ticks attached, while the distribution of the number of animals with a higher level of infestation tended to follow the 80/20 rule. A notably higher level of infestation was detected in males as compared to females. Nymphs were the predominant developmental stage of ticks parasitizing the inspected European ground squirrel specimens. The majority of removed ticks belonged to the nidicolous species Ixodes laguri (79%), while the remaining ticks were identified as Haemaphysalis concinna. In seeking molecular confirmation of morphological identification of Ixodes specimens, COI gene sequences of I. laguri were obtained and deposited in GenBank.
PB  - Inst Zoology, Bas, Sofia
T2  - Acta Zoologica Bulgarica
T1  - Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia
EP  - 553
IS  - 4
SP  - 547
VL  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimi_777
ER  - 

@article{
author = "Radulović, Željko and Mihaljica, Darko and Ćosić, Nada and Penezić, Aleksandra and Ćakić, Sanja and Sukara, Ratko and Ćirović, Duško and Tomanović, Snežana",
year = "2017",
abstract = "Specimens of the endangered species Spermophilus citellus (European ground squirrel) from populations inhabiting 12 localities in Vojvodina (Serbia) were inspected for the presence of attached hard ticks. A total of 1009 ticks were found on 151 infested animals. With respect to the level of infestation, over 60% of the squirrels were found with five or less ticks attached, while the distribution of the number of animals with a higher level of infestation tended to follow the 80/20 rule. A notably higher level of infestation was detected in males as compared to females. Nymphs were the predominant developmental stage of ticks parasitizing the inspected European ground squirrel specimens. The majority of removed ticks belonged to the nidicolous species Ixodes laguri (79%), while the remaining ticks were identified as Haemaphysalis concinna. In seeking molecular confirmation of morphological identification of Ixodes specimens, COI gene sequences of I. laguri were obtained and deposited in GenBank.",
publisher = "Inst Zoology, Bas, Sofia",
journal = "Acta Zoologica Bulgarica",
title = "Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia",
pages = "553-547",
number = "4",
volume = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimi_777"
}

Radulović, Ž., Mihaljica, D., Ćosić, N., Penezić, A., Ćakić, S., Sukara, R., Ćirović, D.,& Tomanović, S.. (2017). Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia. in Acta Zoologica Bulgarica
Inst Zoology, Bas, Sofia., 69(4), 547-553.
https://hdl.handle.net/21.15107/rcub_rimi_777

Radulović Ž, Mihaljica D, Ćosić N, Penezić A, Ćakić S, Sukara R, Ćirović D, Tomanović S. Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia. in Acta Zoologica Bulgarica. 2017;69(4):547-553.
https://hdl.handle.net/21.15107/rcub_rimi_777 .

Radulović, Željko, Mihaljica, Darko, Ćosić, Nada, Penezić, Aleksandra, Ćakić, Sanja, Sukara, Ratko, Ćirović, Duško, Tomanović, Snežana, "Hard Ticks Parasitizing European Ground Squirrel, Spermophilus citellus (L.,1766) (Rodentia: Sciuridae) in Serbia" in Acta Zoologica Bulgarica, 69, no. 4 (2017):547-553,
https://hdl.handle.net/21.15107/rcub_rimi_777 .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Radulović, Eljko
AU  - Mulenga, Albert
AU  - Ćakić, Sanja
AU  - Sukara, Ratko
AU  - Samardzić, Jelena
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/816
AB  - Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks & Tick-Borne Diseases
T1  - Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation
EP  - 395
IS  - 3
SP  - 391
VL  - 8
DO  - 10.1016/j.ttbdis.2017.01.001
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Radulović, Eljko and Mulenga, Albert and Ćakić, Sanja and Sukara, Ratko and Samardzić, Jelena and Tomanović, Snežana",
year = "2017",
abstract = "Tick bites often go unnoticed, so specific reliable tests are needed to confirm them for prompt diagnosis and treatment of tick-borne diseases. One of the promising candidates for developing such a test is AV422, a tick saliva protein that has been conserved across tick genera. In this study, we demonstrate the potential of the AV422 homologue from Ixodes ricinus to be used for tick bite detection for both Prostriata and Metastriata. We expressed recombinant (r) I. ricinus (Ir) AV422 in E. coli and subjected it to Western blot analysis using rat antibodies to saliva proteins of both I. ricinus (Prostriata) and Dermacentor reticulatus (Metastriata) larvae. Our data demonstrate that rIrAV422 specifically bound to antibodies from sera of rats used for both I. ricinus and D. reticulatus larvae feeding, but not to antibodies from control serum, emphasizing its specificity since tick bites were the sole cause of sera reactivity.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks & Tick-Borne Diseases",
title = "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation",
pages = "395-391",
number = "3",
volume = "8",
doi = "10.1016/j.ttbdis.2017.01.001"
}

Mihaljica, D., Marković, D., Radulović, E., Mulenga, A., Ćakić, S., Sukara, R., Samardzić, J.,& Tomanović, S.. (2017). Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 8(3), 391-395.
https://doi.org/10.1016/j.ttbdis.2017.01.001

Mihaljica D, Marković D, Radulović E, Mulenga A, Ćakić S, Sukara R, Samardzić J, Tomanović S. Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation. in Ticks & Tick-Borne Diseases. 2017;8(3):391-395.
doi:10.1016/j.ttbdis.2017.01.001 .

Mihaljica, Darko, Marković, Dragana, Radulović, Eljko, Mulenga, Albert, Ćakić, Sanja, Sukara, Ratko, Samardzić, Jelena, Tomanović, Snežana, "Ixodes ricinus immunogenic saliva protein, homologue to Amblyomma americanum AV422: Determining its potential for use in tick bite confirmation" in Ticks & Tick-Borne Diseases, 8, no. 3 (2017):391-395,
https://doi.org/10.1016/j.ttbdis.2017.01.001 . .

TY  - JOUR
AU  - Radulović, Željko
AU  - Mihaljica, Darko
AU  - Ćosić, Nada
AU  - Penezić, Aleksandra
AU  - Ćakić, Sanja.
AU  - Sukara, Ratko
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/831
AB  - Specimens of the endangered species Spermophilus citellus (European ground squirrel) from populations inhabiting 12 localities in Vojvodina (Serbia) were inspected for the presence of attached hard ticks. A total of 1009 ticks were found on 151 infested animals. With respect to the level of infestation, over 60% of the squirrels were found with five or less ticks attached, while the distribution of the number of animals with a higher level of infestation tended to follow the 80/20 rule. A notably higher level of infestation was detected in males as compared to females. Nymphs were the predominant developmental stage of ticks parasitizing the inspected European ground squirrel specimens. The majority of removed ticks belonged to the nidicolous species Ixodes laguri (79%), while the remaining ticks were identified as Haemaphysalis concinna. In seeking molecular confirmation of morphological identification of Ixodes specimens, COI gene sequences of I. laguri were obtained and deposited in GenBank.
T2  - Acta Zoologica Bulgarica
T1  - Hard ticks parasitizing european ground squirrel, spermophilus citellus (L., 1766) (Rodentia: Sciuridae) in Serbia
EP  - 554
IS  - 4
SP  - 547
VL  - 69
UR  - https://hdl.handle.net/21.15107/rcub_rimi_831
ER  - 

@article{
author = "Radulović, Željko and Mihaljica, Darko and Ćosić, Nada and Penezić, Aleksandra and Ćakić, Sanja. and Sukara, Ratko and Ćirović, Duško and Tomanović, Snežana",
year = "2017",
abstract = "Specimens of the endangered species Spermophilus citellus (European ground squirrel) from populations inhabiting 12 localities in Vojvodina (Serbia) were inspected for the presence of attached hard ticks. A total of 1009 ticks were found on 151 infested animals. With respect to the level of infestation, over 60% of the squirrels were found with five or less ticks attached, while the distribution of the number of animals with a higher level of infestation tended to follow the 80/20 rule. A notably higher level of infestation was detected in males as compared to females. Nymphs were the predominant developmental stage of ticks parasitizing the inspected European ground squirrel specimens. The majority of removed ticks belonged to the nidicolous species Ixodes laguri (79%), while the remaining ticks were identified as Haemaphysalis concinna. In seeking molecular confirmation of morphological identification of Ixodes specimens, COI gene sequences of I. laguri were obtained and deposited in GenBank.",
journal = "Acta Zoologica Bulgarica",
title = "Hard ticks parasitizing european ground squirrel, spermophilus citellus (L., 1766) (Rodentia: Sciuridae) in Serbia",
pages = "554-547",
number = "4",
volume = "69",
url = "https://hdl.handle.net/21.15107/rcub_rimi_831"
}

Radulović, Ž., Mihaljica, D., Ćosić, N., Penezić, A., Ćakić, Sanja., Sukara, R., Ćirović, D.,& Tomanović, S.. (2017). Hard ticks parasitizing european ground squirrel, spermophilus citellus (L., 1766) (Rodentia: Sciuridae) in Serbia. in Acta Zoologica Bulgarica, 69(4), 547-554.
https://hdl.handle.net/21.15107/rcub_rimi_831

Radulović Ž, Mihaljica D, Ćosić N, Penezić A, Ćakić S, Sukara R, Ćirović D, Tomanović S. Hard ticks parasitizing european ground squirrel, spermophilus citellus (L., 1766) (Rodentia: Sciuridae) in Serbia. in Acta Zoologica Bulgarica. 2017;69(4):547-554.
https://hdl.handle.net/21.15107/rcub_rimi_831 .

Radulović, Željko, Mihaljica, Darko, Ćosić, Nada, Penezić, Aleksandra, Ćakić, Sanja., Sukara, Ratko, Ćirović, Duško, Tomanović, Snežana, "Hard ticks parasitizing european ground squirrel, spermophilus citellus (L., 1766) (Rodentia: Sciuridae) in Serbia" in Acta Zoologica Bulgarica, 69, no. 4 (2017):547-554,
https://hdl.handle.net/21.15107/rcub_rimi_831 .

TY  - THES
AU  - Mihaljica, Darko B.
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5722
UR  - http://nardus.mpn.gov.rs/handle/123456789/9382
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:17528/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025183410
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1111
AB  - Assessment of exposure to ticks is a significant data in epidemiological studies of tick-borne diseases, and confirmation of tick bite is important for their diagnosis. In this study, three saliva proteins, PA107, AV422 and calreticulin (CAL), of Ixodes ricinus tick, medically most important species in Europe, were examined in the context of their use for detection of specific antibodies in sera of hosts, as bite markers. Sequence variability analysis revealed high conservativity of all three proteins, with PA107 as the most conservative. Since CAL is ubiquitous in eukaryotes, the further focus of this study was on PA107 and AV422, as proteins that are specific for ticks. Homologues of these proteins from Ixodes ricinus were analyzed for the first time. Reactivity of sera of experimental animals, rats, which were infested separately with Ixodes ricinus or Dermacentor reticulatus larvae, with recombinant forms of IrPA107 and IrAV422 were examined. Specific antibodies were detected in sera of all infested rats using rIrAV422, which implied its usage for detection of bites of tick species that belong to different groups of ixodid ticks, with high sensitivity and specificity. Reactivity with IrPA107 was significantly lower. Hunting dogs, as good sentinels, were used for assessment of rIrAV422 usage for detection of markers in sera of hosts which are exposed to ticks in natural environment. Obtained seroreactivity in all dogs implies broad applicability of this saliva protein, in terms of assessment of exposure to different tick species and risk of infection with tick-borne pathogens.
AB  - Procena izloženosti krpeljima je značajna u epidemiološkim studijama oboljenja čije uzročnike prenose krpelji, a potvrda uboda krpelja je važna za njihovu dijagnostiku. U ovom radu su ispitana tri proteina pljuvačke, PA107, AV422 i kalretikulin (CAL), krpelja Ixodes ricinus, medicinski najznačajnije vrste u Evropi, u kontekstu upotrebe za detekciju specifičnih antitela u serumu domaćina, kao markera uboda. Analiza varijabilnosti njihovih sekvenci je ukazala na visoku konzerviranost sva tri proteina, pri čemu se PA107 pokazao kao najkonzerviraniji. S obzirom na ubikvitarnu zastupljenost CAL kod eukariota, dalji fokus u ovoj studiji je bio na PA107 i AV422, kao proteinima koji su specifični za krpelje i čiji homolozi su prvi put analizirani kod vrste Ixodes ricinus. Ispitivana je reaktivnost seruma eksperimentalnih životinja, pacova, na kojima su hranjene larve Ixodes ricinus ili Dermacentor reticulatus, sa rekombinantnim formama IrPA107 i IrAV422. Korišćenjem rIrAV422, specifična antitela su detektovana kod svih infestiranih pacova, što ukazuje da se rIrAV422 može upotrebiti za detekciju markera uboda različitih grupa tvrdih krpelja uz visoku senzitivnost i specifičnost. Reaktivnost u slučaju IrPA107 je bila značajno manja. Za ispitivanje upotrebe rIrAV422 u detekciji markera uboda u serumima domaćina izloženih krpeljima u prirodnom okruženju, korišćeni su lovački psi kao adekvatna indikatorska grupa. Dobijena seroreaktivnost kod svih analiziranih pasa u studiji ukazuje na širok potencijal primene ovog proteina pljuvačke u proceni izloženosti različitim vrstama krpelja i rizika od infekcije patogenima koje oni prenose.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite
T1  - Potencijal rekombinantnih proteina pljuvačke krpelja Ixodes ricinus (Linnaeus, 1758) za detekciju specifičnih antitela kao markera uboda krpelja
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9382
ER  - 

@phdthesis{
author = "Mihaljica, Darko B.",
year = "2017",
abstract = "Assessment of exposure to ticks is a significant data in epidemiological studies of tick-borne diseases, and confirmation of tick bite is important for their diagnosis. In this study, three saliva proteins, PA107, AV422 and calreticulin (CAL), of Ixodes ricinus tick, medically most important species in Europe, were examined in the context of their use for detection of specific antibodies in sera of hosts, as bite markers. Sequence variability analysis revealed high conservativity of all three proteins, with PA107 as the most conservative. Since CAL is ubiquitous in eukaryotes, the further focus of this study was on PA107 and AV422, as proteins that are specific for ticks. Homologues of these proteins from Ixodes ricinus were analyzed for the first time. Reactivity of sera of experimental animals, rats, which were infested separately with Ixodes ricinus or Dermacentor reticulatus larvae, with recombinant forms of IrPA107 and IrAV422 were examined. Specific antibodies were detected in sera of all infested rats using rIrAV422, which implied its usage for detection of bites of tick species that belong to different groups of ixodid ticks, with high sensitivity and specificity. Reactivity with IrPA107 was significantly lower. Hunting dogs, as good sentinels, were used for assessment of rIrAV422 usage for detection of markers in sera of hosts which are exposed to ticks in natural environment. Obtained seroreactivity in all dogs implies broad applicability of this saliva protein, in terms of assessment of exposure to different tick species and risk of infection with tick-borne pathogens., Procena izloženosti krpeljima je značajna u epidemiološkim studijama oboljenja čije uzročnike prenose krpelji, a potvrda uboda krpelja je važna za njihovu dijagnostiku. U ovom radu su ispitana tri proteina pljuvačke, PA107, AV422 i kalretikulin (CAL), krpelja Ixodes ricinus, medicinski najznačajnije vrste u Evropi, u kontekstu upotrebe za detekciju specifičnih antitela u serumu domaćina, kao markera uboda. Analiza varijabilnosti njihovih sekvenci je ukazala na visoku konzerviranost sva tri proteina, pri čemu se PA107 pokazao kao najkonzerviraniji. S obzirom na ubikvitarnu zastupljenost CAL kod eukariota, dalji fokus u ovoj studiji je bio na PA107 i AV422, kao proteinima koji su specifični za krpelje i čiji homolozi su prvi put analizirani kod vrste Ixodes ricinus. Ispitivana je reaktivnost seruma eksperimentalnih životinja, pacova, na kojima su hranjene larve Ixodes ricinus ili Dermacentor reticulatus, sa rekombinantnim formama IrPA107 i IrAV422. Korišćenjem rIrAV422, specifična antitela su detektovana kod svih infestiranih pacova, što ukazuje da se rIrAV422 može upotrebiti za detekciju markera uboda različitih grupa tvrdih krpelja uz visoku senzitivnost i specifičnost. Reaktivnost u slučaju IrPA107 je bila značajno manja. Za ispitivanje upotrebe rIrAV422 u detekciji markera uboda u serumima domaćina izloženih krpeljima u prirodnom okruženju, korišćeni su lovački psi kao adekvatna indikatorska grupa. Dobijena seroreaktivnost kod svih analiziranih pasa u studiji ukazuje na širok potencijal primene ovog proteina pljuvačke u proceni izloženosti različitim vrstama krpelja i rizika od infekcije patogenima koje oni prenose.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite, Potencijal rekombinantnih proteina pljuvačke krpelja Ixodes ricinus (Linnaeus, 1758) za detekciju specifičnih antitela kao markera uboda krpelja",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9382"
}

Mihaljica, D. B.. (2017). Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_9382

Mihaljica DB. Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_9382 .

Mihaljica, Darko B., "Potential of recombinant Ixodes ricinus (Linnaeus, 1758) tick saliva proteins for detection of specific antibodies as markers of tick bite" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_9382 .

TY  - JOUR
AU  - Burazerović, Jelena
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/654
AB  - Ticks parasitizing bats have been largely understudied, especially in the central part of the Balkan Peninsula, where the last data from the field research date from almost 25 years ago. Bats are hosts to a large number of ectoparasites, including ticks, which can act as vectors of zoonotic agents. For this reason, it is important to identify the distribution of ticks and their relationship to different hosts, including wild animals, bats in particular. The present research was conducted at 16 localities throughout Serbia, Montenegro, Bosnia and Herzegovina, and Former Yugoslav Republic of Macedonia (FYROM). We examined 475 individuals of bats belonging to 13 species. A total of three tick species were identified, I. simplex being the most numerous and widespread, followed by I. vespertilionis and A. vespertilionis. To the best of our knowledge, the presented data include the first records of I. simplex in Serbia and Montenegro, I. vespertilionis for Montenegro and A. vespertilionis in FYROM. Also, we identify a new possible host/parasite association between I. simplex and Rhinolophus euryale.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans
EP  - 291
IS  - 2
SP  - 281
VL  - 66
DO  - 10.1007/s10493-015-9891-6
ER  - 

@article{
author = "Burazerović, Jelena and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Ćirović, Duško and Tomanović, Snežana",
year = "2015",
abstract = "Ticks parasitizing bats have been largely understudied, especially in the central part of the Balkan Peninsula, where the last data from the field research date from almost 25 years ago. Bats are hosts to a large number of ectoparasites, including ticks, which can act as vectors of zoonotic agents. For this reason, it is important to identify the distribution of ticks and their relationship to different hosts, including wild animals, bats in particular. The present research was conducted at 16 localities throughout Serbia, Montenegro, Bosnia and Herzegovina, and Former Yugoslav Republic of Macedonia (FYROM). We examined 475 individuals of bats belonging to 13 species. A total of three tick species were identified, I. simplex being the most numerous and widespread, followed by I. vespertilionis and A. vespertilionis. To the best of our knowledge, the presented data include the first records of I. simplex in Serbia and Montenegro, I. vespertilionis for Montenegro and A. vespertilionis in FYROM. Also, we identify a new possible host/parasite association between I. simplex and Rhinolophus euryale.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans",
pages = "291-281",
number = "2",
volume = "66",
doi = "10.1007/s10493-015-9891-6"
}

Burazerović, J., Ćakić, S., Mihaljica, D., Sukara, R., Ćirović, D.,& Tomanović, S.. (2015). Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans. in Experimental & Applied Acarology
Springer, Dordrecht., 66(2), 281-291.
https://doi.org/10.1007/s10493-015-9891-6

Burazerović J, Ćakić S, Mihaljica D, Sukara R, Ćirović D, Tomanović S. Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans. in Experimental & Applied Acarology. 2015;66(2):281-291.
doi:10.1007/s10493-015-9891-6 .

Burazerović, Jelena, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Ćirović, Duško, Tomanović, Snežana, "Ticks (Acari: Argasidae, Ixodidae) parasitizing bats in the central Balkans" in Experimental & Applied Acarology, 66, no. 2 (2015):281-291,
https://doi.org/10.1007/s10493-015-9891-6 . .

TY  - JOUR
AU  - Hornok, Sandor
AU  - Estrada-Pena, Agustin
AU  - Kontschan, Jeno
AU  - Plantard, Olivier
AU  - Kunz, Bernd
AU  - Mihalca, Andrei D.
AU  - Thabah, Adora
AU  - Tomanović, Snežana
AU  - Burazerović, Jelena
AU  - Takacs, Nora
AU  - Goerfoel, Tamas
AU  - Estok, Peter
AU  - Vuong, Tan Tu
AU  - Szoke, Krisztina
AU  - Fernandez de Mera, Isabel G.
AU  - de la Fuente, Jose
AU  - Takahashi, Mamoru
AU  - Yamauchi, Takeo
AU  - Takano, Ai
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/629
AB  - Background: Phylogeographical studies allow precise genetic comparison of specimens, which were collected over large geographical ranges and belong to the same or closely related animal species. These methods have also been used to compare ticks of veterinary-medical importance. However, relevant data are missing in the case of ixodid ticks of bats, despite (1) the vast geographical range of both Ixodes vespertilionis and Ixodes simplex, and (2) the considerable uncertainty in their taxonomy, which is currently unresolvable by morphological clues. Methods: In the present study 21 ticks were selected from collections or were freshly removed from bats or cave walls in six European and four Asian countries. The DNA was extracted and PCRs were performed to amplify part of the cytochrome oxidase I (COI), 16S and 12S rDNA genes, followed by sequencing for identification and molecular-phylogenetic comparison. Results: No morphological differences were observed between Ixodes vespertilionis specimens from Spain and from other parts of Europe, but corresponding genotypes had only 94.6 % COI sequence identity. An I. vespertilionis specimen collected in Vietnam was different both morphologically and genetically (i.e. with only 84.1 % COI sequence identity in comparison with I. vespertilionis from Europe). Two ticks (collected in Vietnam and in Japan) formed a monophyletic clade and shared morphological features with I. ariadnae, recently described and hitherto only reported in Europe. In addition, two Asiatic specimens of I. simplex were shown to differ markedly from European genotypes of the same species. Phylogenetic relationships of ticks showed similar clustering patterns with those of their associated bat host species. Conclusions: Although all three ixodid bat tick species evaluated in the present study appear to be widespread in Eurasia, they exhibit pronounced genetic differences. Data of this study also reflect that I. vespertilionis may represent a species complex.
PB  - Biomed Central Ltd, London
T2  - Parasites & Vectors
T1  - High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia
VL  - 8
DO  - 10.1186/s13071-015-1056-2
ER  - 

@article{
author = "Hornok, Sandor and Estrada-Pena, Agustin and Kontschan, Jeno and Plantard, Olivier and Kunz, Bernd and Mihalca, Andrei D. and Thabah, Adora and Tomanović, Snežana and Burazerović, Jelena and Takacs, Nora and Goerfoel, Tamas and Estok, Peter and Vuong, Tan Tu and Szoke, Krisztina and Fernandez de Mera, Isabel G. and de la Fuente, Jose and Takahashi, Mamoru and Yamauchi, Takeo and Takano, Ai",
year = "2015",
abstract = "Background: Phylogeographical studies allow precise genetic comparison of specimens, which were collected over large geographical ranges and belong to the same or closely related animal species. These methods have also been used to compare ticks of veterinary-medical importance. However, relevant data are missing in the case of ixodid ticks of bats, despite (1) the vast geographical range of both Ixodes vespertilionis and Ixodes simplex, and (2) the considerable uncertainty in their taxonomy, which is currently unresolvable by morphological clues. Methods: In the present study 21 ticks were selected from collections or were freshly removed from bats or cave walls in six European and four Asian countries. The DNA was extracted and PCRs were performed to amplify part of the cytochrome oxidase I (COI), 16S and 12S rDNA genes, followed by sequencing for identification and molecular-phylogenetic comparison. Results: No morphological differences were observed between Ixodes vespertilionis specimens from Spain and from other parts of Europe, but corresponding genotypes had only 94.6 % COI sequence identity. An I. vespertilionis specimen collected in Vietnam was different both morphologically and genetically (i.e. with only 84.1 % COI sequence identity in comparison with I. vespertilionis from Europe). Two ticks (collected in Vietnam and in Japan) formed a monophyletic clade and shared morphological features with I. ariadnae, recently described and hitherto only reported in Europe. In addition, two Asiatic specimens of I. simplex were shown to differ markedly from European genotypes of the same species. Phylogenetic relationships of ticks showed similar clustering patterns with those of their associated bat host species. Conclusions: Although all three ixodid bat tick species evaluated in the present study appear to be widespread in Eurasia, they exhibit pronounced genetic differences. Data of this study also reflect that I. vespertilionis may represent a species complex.",
publisher = "Biomed Central Ltd, London",
journal = "Parasites & Vectors",
title = "High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia",
volume = "8",
doi = "10.1186/s13071-015-1056-2"
}

Hornok, S., Estrada-Pena, A., Kontschan, J., Plantard, O., Kunz, B., Mihalca, A. D., Thabah, A., Tomanović, S., Burazerović, J., Takacs, N., Goerfoel, T., Estok, P., Vuong, T. T., Szoke, K., Fernandez de Mera, I. G., de la Fuente, J., Takahashi, M., Yamauchi, T.,& Takano, A.. (2015). High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia. in Parasites & Vectors
Biomed Central Ltd, London., 8.
https://doi.org/10.1186/s13071-015-1056-2

Hornok S, Estrada-Pena A, Kontschan J, Plantard O, Kunz B, Mihalca AD, Thabah A, Tomanović S, Burazerović J, Takacs N, Goerfoel T, Estok P, Vuong TT, Szoke K, Fernandez de Mera IG, de la Fuente J, Takahashi M, Yamauchi T, Takano A. High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia. in Parasites & Vectors. 2015;8.
doi:10.1186/s13071-015-1056-2 .

Hornok, Sandor, Estrada-Pena, Agustin, Kontschan, Jeno, Plantard, Olivier, Kunz, Bernd, Mihalca, Andrei D., Thabah, Adora, Tomanović, Snežana, Burazerović, Jelena, Takacs, Nora, Goerfoel, Tamas, Estok, Peter, Vuong, Tan Tu, Szoke, Krisztina, Fernandez de Mera, Isabel G., de la Fuente, Jose, Takahashi, Mamoru, Yamauchi, Takeo, Takano, Ai, "High degree of mitochondrial gene heterogeneity in the bat tick species Ixodes vespertilionis, I. ariadnae and I. simplex from Eurasia" in Parasites & Vectors, 8 (2015),
https://doi.org/10.1186/s13071-015-1056-2 . .

TY  - JOUR
AU  - Hornok, Sandor
AU  - Kontschan, Jeno
AU  - Estrada-Pena, Agustin
AU  - Fernandez de Mera, Isabel G.
AU  - Tomanović, Snežana
AU  - de la Fuente, Jose
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/676
AB  - Background: Recently a new hard tick species, Ixodes ariadnae has been discovered, adding to the two known ixodid tick species (I. vespertilionis and I. simplex) of bats in Europe. Findings: Scanning electron microscopic comparison of adult females of these species shows morphological differences concerning the palps, the scutum, the Haller's organ, the coxae, as well as the arrangement and fine structure of setae. Molecular analysis of 10 geographically different isolates revealed 90-95% sequence homology in the 12S and 16S rDNA genes of bat tick species. Based on 12S rDNA sequences, genotypes of I. ariadnae clustered closest to I. simplex, whereas according to their 16S rDNA gene they were closest to I. vespertilionis. The subolesin gene of I. ariadnae had only 91% sequence homology with that of I. ricinus, and is the longest known among hard tick species. Conclusions: The present study illustrates the morphology and clarifies the phylogenetic relationships of the three known bat tick species that occur in Europe. According to its subolesin gene I. ariadnae may have a long evolutionary history.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Contributions to the morphology and phylogeny of the newly discovered bat tick species, Ixodes ariadnae in comparison with I. vespertilionis and I. simplex
VL  - 8
DO  - 10.1186/s13071-015-0665-0
ER  - 

@article{
author = "Hornok, Sandor and Kontschan, Jeno and Estrada-Pena, Agustin and Fernandez de Mera, Isabel G. and Tomanović, Snežana and de la Fuente, Jose",
year = "2015",
abstract = "Background: Recently a new hard tick species, Ixodes ariadnae has been discovered, adding to the two known ixodid tick species (I. vespertilionis and I. simplex) of bats in Europe. Findings: Scanning electron microscopic comparison of adult females of these species shows morphological differences concerning the palps, the scutum, the Haller's organ, the coxae, as well as the arrangement and fine structure of setae. Molecular analysis of 10 geographically different isolates revealed 90-95% sequence homology in the 12S and 16S rDNA genes of bat tick species. Based on 12S rDNA sequences, genotypes of I. ariadnae clustered closest to I. simplex, whereas according to their 16S rDNA gene they were closest to I. vespertilionis. The subolesin gene of I. ariadnae had only 91% sequence homology with that of I. ricinus, and is the longest known among hard tick species. Conclusions: The present study illustrates the morphology and clarifies the phylogenetic relationships of the three known bat tick species that occur in Europe. According to its subolesin gene I. ariadnae may have a long evolutionary history.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Contributions to the morphology and phylogeny of the newly discovered bat tick species, Ixodes ariadnae in comparison with I. vespertilionis and I. simplex",
volume = "8",
doi = "10.1186/s13071-015-0665-0"
}

Hornok, S., Kontschan, J., Estrada-Pena, A., Fernandez de Mera, I. G., Tomanović, S.,& de la Fuente, J.. (2015). Contributions to the morphology and phylogeny of the newly discovered bat tick species, Ixodes ariadnae in comparison with I. vespertilionis and I. simplex. in Parasites & Vectors
BMC, London., 8.
https://doi.org/10.1186/s13071-015-0665-0

Hornok S, Kontschan J, Estrada-Pena A, Fernandez de Mera IG, Tomanović S, de la Fuente J. Contributions to the morphology and phylogeny of the newly discovered bat tick species, Ixodes ariadnae in comparison with I. vespertilionis and I. simplex. in Parasites & Vectors. 2015;8.
doi:10.1186/s13071-015-0665-0 .

Hornok, Sandor, Kontschan, Jeno, Estrada-Pena, Agustin, Fernandez de Mera, Isabel G., Tomanović, Snežana, de la Fuente, Jose, "Contributions to the morphology and phylogeny of the newly discovered bat tick species, Ixodes ariadnae in comparison with I. vespertilionis and I. simplex" in Parasites & Vectors, 8 (2015),
https://doi.org/10.1186/s13071-015-0665-0 . .

TY  - JOUR
AU  - Obrenović, Sonja
AU  - Ristanović, Elizabeta
AU  - Cekanac, Radovan
AU  - Radulović, Željko
AU  - Ilić, Vesna
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/657
AB  - In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA
EP  - 110
IS  - 1
SP  - 99
VL  - 65
DO  - 10.1515/acve-2015-0008
ER  - 

@article{
author = "Obrenović, Sonja and Ristanović, Elizabeta and Cekanac, Radovan and Radulović, Željko and Ilić, Vesna",
year = "2015",
abstract = "In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA",
pages = "110-99",
number = "1",
volume = "65",
doi = "10.1515/acve-2015-0008"
}

Obrenović, S., Ristanović, E., Cekanac, R., Radulović, Ž.,& Ilić, V.. (2015). SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 99-110.
https://doi.org/10.1515/acve-2015-0008

Obrenović S, Ristanović E, Cekanac R, Radulović Ž, Ilić V. SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA. in Acta Veterinaria-Beograd. 2015;65(1):99-110.
doi:10.1515/acve-2015-0008 .

Obrenović, Sonja, Ristanović, Elizabeta, Cekanac, Radovan, Radulović, Željko, Ilić, Vesna, "SEROPREVALENCE OF IgG ANTIBODIES AGAINST BORRELIA BURGDORFERI IN DOGS IN BELGRADE AREA, SERBIA" in Acta Veterinaria-Beograd, 65, no. 1 (2015):99-110,
https://doi.org/10.1515/acve-2015-0008 . .

TY  - JOUR
AU  - Rakhshani, Ehsan
AU  - Stary, Petr
AU  - Perez Hidalgo, Nicolas
AU  - Ckrkić, Jelisaveta
AU  - Moghaddam, Mostafa Ghafouri
AU  - Tomanović, Snežana
AU  - Petrović, Anđeljko
AU  - Tomanović, Željko
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/606
AB  - The present paper represents a contribution to the knowledge of the taxonomy of Monoctonia Stary aphid parasitoids obtained using the barcoding region of the mitochondrial COI gene. We discuss the phylogenetic position of the genus within the subtribe Monoctonina, redescribe known species, and describe Monoctonia japonica sp. n. from Japan in the association Pemphigus matsumurai Monzen/Populus maximowiczii. A key for species identification is provided. Also, we review and discuss the host records, origin, and geographical distribution of Monoctonia species. It is hypothesized that the genus Monoctonia evolved in Paleogene forests of the temperate (and subtropical) belt, most probably in the European part of the Mediterranean region, which is also the center of origin of their host plants.
PB  - Magnolia Press, Auckland
T2  - Zootaxa
T1  - Revision of the world Monoctonia Stary, parasitoids of gall aphids: taxonomy, distribution, host range, and phylogeny (Hymenoptera, Braconidae: Aphidiinae)
EP  - 488
IS  - 4
SP  - 474
VL  - 3905
DO  - 10.11646/zootaxa.3905.4.2
ER  - 

@article{
author = "Rakhshani, Ehsan and Stary, Petr and Perez Hidalgo, Nicolas and Ckrkić, Jelisaveta and Moghaddam, Mostafa Ghafouri and Tomanović, Snežana and Petrović, Anđeljko and Tomanović, Željko",
year = "2015",
abstract = "The present paper represents a contribution to the knowledge of the taxonomy of Monoctonia Stary aphid parasitoids obtained using the barcoding region of the mitochondrial COI gene. We discuss the phylogenetic position of the genus within the subtribe Monoctonina, redescribe known species, and describe Monoctonia japonica sp. n. from Japan in the association Pemphigus matsumurai Monzen/Populus maximowiczii. A key for species identification is provided. Also, we review and discuss the host records, origin, and geographical distribution of Monoctonia species. It is hypothesized that the genus Monoctonia evolved in Paleogene forests of the temperate (and subtropical) belt, most probably in the European part of the Mediterranean region, which is also the center of origin of their host plants.",
publisher = "Magnolia Press, Auckland",
journal = "Zootaxa",
title = "Revision of the world Monoctonia Stary, parasitoids of gall aphids: taxonomy, distribution, host range, and phylogeny (Hymenoptera, Braconidae: Aphidiinae)",
pages = "488-474",
number = "4",
volume = "3905",
doi = "10.11646/zootaxa.3905.4.2"
}

Rakhshani, E., Stary, P., Perez Hidalgo, N., Ckrkić, J., Moghaddam, M. G., Tomanović, S., Petrović, A.,& Tomanović, Ž.. (2015). Revision of the world Monoctonia Stary, parasitoids of gall aphids: taxonomy, distribution, host range, and phylogeny (Hymenoptera, Braconidae: Aphidiinae). in Zootaxa
Magnolia Press, Auckland., 3905(4), 474-488.
https://doi.org/10.11646/zootaxa.3905.4.2

Rakhshani E, Stary P, Perez Hidalgo N, Ckrkić J, Moghaddam MG, Tomanović S, Petrović A, Tomanović Ž. Revision of the world Monoctonia Stary, parasitoids of gall aphids: taxonomy, distribution, host range, and phylogeny (Hymenoptera, Braconidae: Aphidiinae). in Zootaxa. 2015;3905(4):474-488.
doi:10.11646/zootaxa.3905.4.2 .

Rakhshani, Ehsan, Stary, Petr, Perez Hidalgo, Nicolas, Ckrkić, Jelisaveta, Moghaddam, Mostafa Ghafouri, Tomanović, Snežana, Petrović, Anđeljko, Tomanović, Željko, "Revision of the world Monoctonia Stary, parasitoids of gall aphids: taxonomy, distribution, host range, and phylogeny (Hymenoptera, Braconidae: Aphidiinae)" in Zootaxa, 3905, no. 4 (2015):474-488,
https://doi.org/10.11646/zootaxa.3905.4.2 . .

TY  - JOUR
AU  - Ćirović, Duško
AU  - Chochlakis, Dimosthenis
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Penezić, Aleksandra
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/596
AB  - The golden jackal Canis aureus occurs in south-eastern Europe, Asia, the Middle East, the Caucasus, and Africa. In Serbia, jackals neared extinction; however, during the last 30 years, the species started to spread quickly and to increase in number. Few studies in the past have revealed their potential role as carriers of zoonotic diseases. Animal samples were collected over a three-year period (01/2010-02/2013) from 12 sites all over Serbia. Of the tissue samples collected, spleen was chosen as the tissue to proceed; all samples were tested for Leishmania species and Brucella species by real-time PCR. Of the 216 samples collected, 15 (6.9%) were positive for Leishmania species, while four (1.9%) were positive for B. canis. The potential epidemiologic role of the golden jackal in carrying and dispersing zoonotic diseases in Serbia should be taken under consideration when applying surveillance monitoring schemes.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - Presence of Leishmania and Brucella Species in the Golden Jackal Canis aureus in Serbia
VL  - 2014
DO  - 10.1155/2014/728516
ER  - 

@article{
author = "Ćirović, Duško and Chochlakis, Dimosthenis and Tomanović, Snežana and Sukara, Ratko and Penezić, Aleksandra and Tselentis, Yannis and Psaroulaki, Anna",
year = "2014",
abstract = "The golden jackal Canis aureus occurs in south-eastern Europe, Asia, the Middle East, the Caucasus, and Africa. In Serbia, jackals neared extinction; however, during the last 30 years, the species started to spread quickly and to increase in number. Few studies in the past have revealed their potential role as carriers of zoonotic diseases. Animal samples were collected over a three-year period (01/2010-02/2013) from 12 sites all over Serbia. Of the tissue samples collected, spleen was chosen as the tissue to proceed; all samples were tested for Leishmania species and Brucella species by real-time PCR. Of the 216 samples collected, 15 (6.9%) were positive for Leishmania species, while four (1.9%) were positive for B. canis. The potential epidemiologic role of the golden jackal in carrying and dispersing zoonotic diseases in Serbia should be taken under consideration when applying surveillance monitoring schemes.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "Presence of Leishmania and Brucella Species in the Golden Jackal Canis aureus in Serbia",
volume = "2014",
doi = "10.1155/2014/728516"
}

Ćirović, D., Chochlakis, D., Tomanović, S., Sukara, R., Penezić, A., Tselentis, Y.,& Psaroulaki, A.. (2014). Presence of Leishmania and Brucella Species in the Golden Jackal Canis aureus in Serbia. in Biomed Research International
Hindawi Ltd, London., 2014.
https://doi.org/10.1155/2014/728516

Ćirović D, Chochlakis D, Tomanović S, Sukara R, Penezić A, Tselentis Y, Psaroulaki A. Presence of Leishmania and Brucella Species in the Golden Jackal Canis aureus in Serbia. in Biomed Research International. 2014;2014.
doi:10.1155/2014/728516 .

Ćirović, Duško, Chochlakis, Dimosthenis, Tomanović, Snežana, Sukara, Ratko, Penezić, Aleksandra, Tselentis, Yannis, Psaroulaki, Anna, "Presence of Leishmania and Brucella Species in the Golden Jackal Canis aureus in Serbia" in Biomed Research International, 2014 (2014),
https://doi.org/10.1155/2014/728516 . .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Mojsilović, Miljana
AU  - Mihaljica, Darko
AU  - Milutinović, Marija
AU  - Petrović, Anđeljko
AU  - Tomanović, Snežana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/551
AB  - The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia
EP  - 690
IS  - 2
SP  - 683
VL  - 66
DO  - 10.2298/ABS1402683C
ER  - 

@article{
author = "Ćakić, Sanja and Mojsilović, Miljana and Mihaljica, Darko and Milutinović, Marija and Petrović, Anđeljko and Tomanović, Snežana",
year = "2014",
abstract = "The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia",
pages = "690-683",
number = "2",
volume = "66",
doi = "10.2298/ABS1402683C"
}

Ćakić, S., Mojsilović, M., Mihaljica, D., Milutinović, M., Petrović, A.,& Tomanović, S.. (2014). Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 683-690.
https://doi.org/10.2298/ABS1402683C

Ćakić S, Mojsilović M, Mihaljica D, Milutinović M, Petrović A, Tomanović S. Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia. in Archives of Biological Sciences. 2014;66(2):683-690.
doi:10.2298/ABS1402683C .

Ćakić, Sanja, Mojsilović, Miljana, Mihaljica, Darko, Milutinović, Marija, Petrović, Anđeljko, Tomanović, Snežana, "Molecular characterization of coi gene of ixodes ricinus (linnaeus, 1758) from Serbia" in Archives of Biological Sciences, 66, no. 2 (2014):683-690,
https://doi.org/10.2298/ABS1402683C . .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Mojsilović, Miljana
AU  - Mihaljica, Darko
AU  - Milutinović, Marija
AU  - Petrović, Anđeljko
AU  - Tomanović, Snežana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/578
AB  - The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia
EP  - 1251
IS  - 3
SP  - 1243
VL  - 66
DO  - 10.2298/ABS1403243C
ER  - 

@article{
author = "Ćakić, Sanja and Mojsilović, Miljana and Mihaljica, Darko and Milutinović, Marija and Petrović, Anđeljko and Tomanović, Snežana",
year = "2014",
abstract = "The Ixodes ricinus tick is common in the central part of the Balkan Peninsula. It is a vector of pathogenic agents causing diseases in humans and animals. Little is known about the genetic structure of I. ricinus in this region. We have investigated intraspecific variability of the COI gene among I. ricinus ticks collected from different regions of Serbia, and the correlation between the various types of habitat and genetic variability of ticks. The obtained COI gene sequences are the first barcoding sequences of I. ricinus ticks collected at localities in Serbia. Intraspecific variability of these COI gene sequences was very low, and there was no correlation between the various types of habitat and genetic variability of ticks. Samples from isolated localities (canyon/gorge) showed no genetic differentiations from the majority of samples from open areas.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia",
pages = "1251-1243",
number = "3",
volume = "66",
doi = "10.2298/ABS1403243C"
}

Ćakić, S., Mojsilović, M., Mihaljica, D., Milutinović, M., Petrović, A.,& Tomanović, S.. (2014). Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(3), 1243-1251.
https://doi.org/10.2298/ABS1403243C

Ćakić S, Mojsilović M, Mihaljica D, Milutinović M, Petrović A, Tomanović S. Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia. in Archives of Biological Sciences. 2014;66(3):1243-1251.
doi:10.2298/ABS1403243C .

Ćakić, Sanja, Mojsilović, Miljana, Mihaljica, Darko, Milutinović, Marija, Petrović, Anđeljko, Tomanović, Snežana, "Molecular characterization of COI gene of Ixodes ricinus (Linnaeus, 1758) from Serbia" in Archives of Biological Sciences, 66, no. 3 (2014):1243-1251,
https://doi.org/10.2298/ABS1403243C . .

TY  - JOUR
AU  - Stary, Petr
AU  - Kavallieratos, Nickolas G.
AU  - Petrović, Anđeljko
AU  - Zikić, Vladimir
AU  - Rakhshani, Ehsan
AU  - Tomanović, Snežana
AU  - Tomanović, Željko
AU  - Havelka, Jan
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/526
AB  - This study provides evidence on integrating the morphological, field, and laboratory data, and application of the cytochrome oxidase subunit I (COI) barcoding gene to the three asexual or sexual Lysiphlebus spp., i.e., Lysiphlebus cardui (Marshall), Lysiphlebus confusus Tremblay and Eady and Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae). New aphid-invasive plant association, Aphis fabae Scopoli (Hemipreta: Aphididae) on Impatiens glandulifera Royle, has been used in the same model area in the Czech Republic under the same sampling and rearing method for several consecutive years and throughout the season. For molecular identification of these three species, we used DNA sequences of the barcoding region of the mitochondrial COI gene. Although our results confirmed ecological and morphological differences among L. cardui, L. confusus, and L. fabarum, genetic analysis on the basis of COI mitochondrial barcoding gene does not support species status of the mentioned Lysiphlebus taxa. The level of morphological differentiation in these Lysiphlebus Forster species is in accordance with the usual species variability within subfamily Aphidiinae. However, it should be examined how appearance of asexual lineages affects the morphological or genetical variability.
PB  - Oxford Univ Press Inc, Cary
T2  - Journal of Insect Science
T1  - Interference of Field Evidence, Morphology, and DNA Analyses of Three Related Lysiphlebus Aphid Parasitoids (Hymenoptera: Braconidae: Aphidiinae)
VL  - 14
DO  - 10.1093/jisesa/ieu033
ER  - 

@article{
author = "Stary, Petr and Kavallieratos, Nickolas G. and Petrović, Anđeljko and Zikić, Vladimir and Rakhshani, Ehsan and Tomanović, Snežana and Tomanović, Željko and Havelka, Jan",
year = "2014",
abstract = "This study provides evidence on integrating the morphological, field, and laboratory data, and application of the cytochrome oxidase subunit I (COI) barcoding gene to the three asexual or sexual Lysiphlebus spp., i.e., Lysiphlebus cardui (Marshall), Lysiphlebus confusus Tremblay and Eady and Lysiphlebus fabarum (Marshall) (Hymenoptera: Braconidae: Aphidiinae). New aphid-invasive plant association, Aphis fabae Scopoli (Hemipreta: Aphididae) on Impatiens glandulifera Royle, has been used in the same model area in the Czech Republic under the same sampling and rearing method for several consecutive years and throughout the season. For molecular identification of these three species, we used DNA sequences of the barcoding region of the mitochondrial COI gene. Although our results confirmed ecological and morphological differences among L. cardui, L. confusus, and L. fabarum, genetic analysis on the basis of COI mitochondrial barcoding gene does not support species status of the mentioned Lysiphlebus taxa. The level of morphological differentiation in these Lysiphlebus Forster species is in accordance with the usual species variability within subfamily Aphidiinae. However, it should be examined how appearance of asexual lineages affects the morphological or genetical variability.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journal of Insect Science",
title = "Interference of Field Evidence, Morphology, and DNA Analyses of Three Related Lysiphlebus Aphid Parasitoids (Hymenoptera: Braconidae: Aphidiinae)",
volume = "14",
doi = "10.1093/jisesa/ieu033"
}

Stary, P., Kavallieratos, N. G., Petrović, A., Zikić, V., Rakhshani, E., Tomanović, S., Tomanović, Ž.,& Havelka, J.. (2014). Interference of Field Evidence, Morphology, and DNA Analyses of Three Related Lysiphlebus Aphid Parasitoids (Hymenoptera: Braconidae: Aphidiinae). in Journal of Insect Science
Oxford Univ Press Inc, Cary., 14.
https://doi.org/10.1093/jisesa/ieu033

Stary P, Kavallieratos NG, Petrović A, Zikić V, Rakhshani E, Tomanović S, Tomanović Ž, Havelka J. Interference of Field Evidence, Morphology, and DNA Analyses of Three Related Lysiphlebus Aphid Parasitoids (Hymenoptera: Braconidae: Aphidiinae). in Journal of Insect Science. 2014;14.
doi:10.1093/jisesa/ieu033 .

Stary, Petr, Kavallieratos, Nickolas G., Petrović, Anđeljko, Zikić, Vladimir, Rakhshani, Ehsan, Tomanović, Snežana, Tomanović, Željko, Havelka, Jan, "Interference of Field Evidence, Morphology, and DNA Analyses of Three Related Lysiphlebus Aphid Parasitoids (Hymenoptera: Braconidae: Aphidiinae)" in Journal of Insect Science, 14 (2014),
https://doi.org/10.1093/jisesa/ieu033 . .

TY  - JOUR
AU  - Tomanović, Snežana
AU  - Chochlakis, Dimosthenis
AU  - Radulović, Željko
AU  - Milutinović, Marija
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/516
AB  - Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia
EP  - 376
IS  - 3
SP  - 367
VL  - 59
DO  - 10.1007/s10493-012-9597-y
ER  - 

@article{
author = "Tomanović, Snežana and Chochlakis, Dimosthenis and Radulović, Željko and Milutinović, Marija and Ćakić, Sanja and Mihaljica, Darko and Tselentis, Yannis and Psaroulaki, Anna",
year = "2013",
abstract = "Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia",
pages = "376-367",
number = "3",
volume = "59",
doi = "10.1007/s10493-012-9597-y"
}

Tomanović, S., Chochlakis, D., Radulović, Ž., Milutinović, M., Ćakić, S., Mihaljica, D., Tselentis, Y.,& Psaroulaki, A.. (2013). Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia. in Experimental & Applied Acarology
Springer, Dordrecht., 59(3), 367-376.
https://doi.org/10.1007/s10493-012-9597-y

Tomanović S, Chochlakis D, Radulović Ž, Milutinović M, Ćakić S, Mihaljica D, Tselentis Y, Psaroulaki A. Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia. in Experimental & Applied Acarology. 2013;59(3):367-376.
doi:10.1007/s10493-012-9597-y .

Tomanović, Snežana, Chochlakis, Dimosthenis, Radulović, Željko, Milutinović, Marija, Ćakić, Sanja, Mihaljica, Darko, Tselentis, Yannis, Psaroulaki, Anna, "Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia" in Experimental & Applied Acarology, 59, no. 3 (2013):367-376,
https://doi.org/10.1007/s10493-012-9597-y . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Radulović, Željko
AU  - Tomanović, Snežana
AU  - Ćakić, Sanja
AU  - Penezić, Aleksandra
AU  - Milutinović, Marija
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/407
AB  - In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Molecular detection of Babesia spp. in ticks in northern Serbia
EP  - 1598
IS  - 4
SP  - 1591
VL  - 64
DO  - 10.2298/ABS1204591M
ER  - 

@article{
author = "Mihaljica, Darko and Radulović, Željko and Tomanović, Snežana and Ćakić, Sanja and Penezić, Aleksandra and Milutinović, Marija",
year = "2012",
abstract = "In order to evaluate the prevalence rate of Babesia spp. in ticks collected from vegetation at seven localities in northern Serbia, tick samples were subjected to molecular analysis. A total of 132 unfed adult ticks of five different species (Dermacentor marginatus, Dermacentor reticulatus, Ixodes ricinus, Haemaphysalis concinna and Haemaphysalis punctata), were examined by PCR for the presence of Babesia spp. Out of the analyzed ticks, 10.61% (14/132) were positive for babesial DNA. The presence of babesiae was found at the localities Pančevački Rit, Titov Gaj, Makiš, PKB and Kljajićevo. Prevalence in D. reticulatus ticks was 21.57% (11/51) and in H. concinna ticks, 8.57% (3/35). Sequencing and phylogenetic analysis showed a clustering of the obtained sequences with those of B. canis from the GenBank database. These results add to the knowledge of the distribution of babesial pathogens and their vectors in Serbia.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Molecular detection of Babesia spp. in ticks in northern Serbia",
pages = "1598-1591",
number = "4",
volume = "64",
doi = "10.2298/ABS1204591M"
}

Mihaljica, D., Radulović, Ž., Tomanović, S., Ćakić, S., Penezić, A.,& Milutinović, M.. (2012). Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1591-1598.
https://doi.org/10.2298/ABS1204591M

Mihaljica D, Radulović Ž, Tomanović S, Ćakić S, Penezić A, Milutinović M. Molecular detection of Babesia spp. in ticks in northern Serbia. in Archives of Biological Sciences. 2012;64(4):1591-1598.
doi:10.2298/ABS1204591M .

Mihaljica, Darko, Radulović, Željko, Tomanović, Snežana, Ćakić, Sanja, Penezić, Aleksandra, Milutinović, Marija, "Molecular detection of Babesia spp. in ticks in northern Serbia" in Archives of Biological Sciences, 64, no. 4 (2012):1591-1598,
https://doi.org/10.2298/ABS1204591M . .