TY  - JOUR
AU  - Kovačić, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/717
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
EP  - 142
SP  - 135
VL  - 106
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 

@article{
author = "Kovačić, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
pages = "142-135",
volume = "106",
doi = "10.1016/j.rvsc.2016.04.002"
}

Kovačić, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002

Kovačić M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .

Kovačić, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/399
AB  - Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology & Immunopathology
T1  - Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG
EP  - 168
IS  - 3-4
SP  - 161
VL  - 150
DO  - 10.1016/j.vetimm.2012.09.009
ER  - 

@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vuković, Dejan and Savić, Olivera and Kovačić, Marijana and Ilić, Vesna",
year = "2012",
abstract = "Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology & Immunopathology",
title = "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG",
pages = "168-161",
number = "3-4",
volume = "150",
doi = "10.1016/j.vetimm.2012.09.009"
}

Fratrić, N., Gvozdić, D., Vuković, D., Savić, O., Kovačić, M.,& Ilić, V.. (2012). Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology
Elsevier, Amsterdam., 150(3-4), 161-168.
https://doi.org/10.1016/j.vetimm.2012.09.009

Fratrić N, Gvozdić D, Vuković D, Savić O, Kovačić M, Ilić V. Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology. 2012;150(3-4):161-168.
doi:10.1016/j.vetimm.2012.09.009 .

Fratrić, Natalija, Gvozdić, Dragan, Vuković, Dejan, Savić, Olivera, Kovačić, Marijana, Ilić, Vesna, "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG" in Veterinary Immunology & Immunopathology, 150, no. 3-4 (2012):161-168,
https://doi.org/10.1016/j.vetimm.2012.09.009 . .