Vučetić, Dušan

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  • Vučetić, Dušan (6)
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Author's Bibliography

Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi

Mihaljica, Darko; Marković, Dragana; Božić Nedeljković, Biljana; Veinović, Gorana; Sukara, Ratko; Vučetić, Dušan; Krstić, Milena; Mladenović, Jovan; Atanasievska, Sonja; Protić Đokić, Vesna; Đorđevski, Nikoleta; Ristanović, Elizabeta; Tomanović, Snežana

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2023)

TY  - CONF
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Božić Nedeljković, Biljana
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Vučetić, Dušan
AU  - Krstić, Milena
AU  - Mladenović, Jovan
AU  - Atanasievska, Sonja
AU  - Protić Đokić, Vesna
AU  - Đorđevski, Nikoleta
AU  - Ristanović, Elizabeta
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1446
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad
T1  - Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi
EP  - 71
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1446
ER  - 
@conference{
author = "Mihaljica, Darko and Marković, Dragana and Božić Nedeljković, Biljana and Veinović, Gorana and Sukara, Ratko and Vučetić, Dušan and Krstić, Milena and Mladenović, Jovan and Atanasievska, Sonja and Protić Đokić, Vesna and Đorđevski, Nikoleta and Ristanović, Elizabeta and Tomanović, Snežana",
year = "2023",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad",
title = "Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi",
pages = "71-70",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1446"
}
Mihaljica, D., Marković, D., Božić Nedeljković, B., Veinović, G., Sukara, R., Vučetić, D., Krstić, M., Mladenović, J., Atanasievska, S., Protić Đokić, V., Đorđevski, N., Ristanović, E.,& Tomanović, S.. (2023). Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 70-71.
https://hdl.handle.net/21.15107/rcub_rimi_1446
Mihaljica D, Marković D, Božić Nedeljković B, Veinović G, Sukara R, Vučetić D, Krstić M, Mladenović J, Atanasievska S, Protić Đokić V, Đorđevski N, Ristanović E, Tomanović S. Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad. 2023;:70-71.
https://hdl.handle.net/21.15107/rcub_rimi_1446 .
Mihaljica, Darko, Marković, Dragana, Božić Nedeljković, Biljana, Veinović, Gorana, Sukara, Ratko, Vučetić, Dušan, Krstić, Milena, Mladenović, Jovan, Atanasievska, Sonja, Protić Đokić, Vesna, Đorđevski, Nikoleta, Ristanović, Elizabeta, Tomanović, Snežana, "Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad (2023):70-71,
https://hdl.handle.net/21.15107/rcub_rimi_1446 .

Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients

Đukić, Tamara; Drvenica, Ivana; Kovačić, Marijana; Minić, Rajna; Vučetić, Dušan; Majerič, Dragana; Šefik-Bukilica, Mirjana; Savić, Olivera; Bugarski, Branko; Ilić, Vesna

(Elsevier, 2023)

TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Minić, Rajna
AU  - Vučetić, Dušan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Savić, Olivera
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1314
AB  - The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.
PB  - Elsevier
T2  - Analytical Biochemistry
T2  - Analytical BiochemistryAnalytical Biochemistry
T1  - Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients
SP  - 115194
VL  - 674
DO  - 10.1016/j.ab.2023.115194
ER  - 
@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Minić, Rajna and Vučetić, Dušan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Savić, Olivera and Bugarski, Branko and Ilić, Vesna",
year = "2023",
abstract = "The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.",
publisher = "Elsevier",
journal = "Analytical Biochemistry, Analytical BiochemistryAnalytical Biochemistry",
title = "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients",
pages = "115194",
volume = "674",
doi = "10.1016/j.ab.2023.115194"
}
Đukić, T., Drvenica, I., Kovačić, M., Minić, R., Vučetić, D., Majerič, D., Šefik-Bukilica, M., Savić, O., Bugarski, B.,& Ilić, V.. (2023). Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry
Elsevier., 674, 115194.
https://doi.org/10.1016/j.ab.2023.115194
Đukić T, Drvenica I, Kovačić M, Minić R, Vučetić D, Majerič D, Šefik-Bukilica M, Savić O, Bugarski B, Ilić V. Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry. 2023;674:115194.
doi:10.1016/j.ab.2023.115194 .
Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Minić, Rajna, Vučetić, Dušan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Savić, Olivera, Bugarski, Branko, Ilić, Vesna, "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients" in Analytical Biochemistry, 674 (2023):115194,
https://doi.org/10.1016/j.ab.2023.115194 . .
1

Optical methodologies in the analysis of erythrocyte deformability and heterogeneity

Drvenica, Ivana; Trivanović, Drenka; Radmilović, Mihajlo; Vučetić, Dušan; Krmpot, Aleksandar; Ilić, Vesna

(Belgrade: Institute of Physics, 2023)

TY  - CONF
AU  - Drvenica, Ivana
AU  - Trivanović, Drenka
AU  - Radmilović, Mihajlo
AU  - Vučetić, Dušan
AU  - Krmpot, Aleksandar
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1405
AB  - Ektacytometry, a diffraction-based method, measures the deformability of entire erythrocyte populations and does not provide information on the altered deformability of individual erythrocytes or affected subpopulation induced by constant oxidative stress, physical stress, metabolic depletion, and loss of ion gradients during their 120 days life span. We introduced an approach based on ektacytometry coupled to flow cytometry analysis to monitor the deformability and heterogeneity of subpopulations of erythrocytes. The effects of in vitro changes of osmotic gradient (from 155 mM to 93 mM phosphate buffer) and treatment by oxidative agent (0.5 mM and 0.75 mM terc-bytil hydroperoxide (TBPH)) on human erythrocyte isolated from healthy male donors, were tested using RheoScan D 300 (RheoMeditech. Inc., Korea) and BD FACSCalibur flow cytometer (Becton Dickinson, USA).
A decrease in erythrocyte deformability by changes in osmolality or treatment with TBPH 
per se was demonstrated by ektacytometry. Nevertheless, this method could not analyze the 
erythrocytes that underwent both treatments due to their lysis by the shear stress in the 
device. The samples of an equal population of normal erythrocytes and erythrocytes 
rigidified by 0.5 mM TBPH (slightly rigid) showed elongation indices in the physiological 
range, i.e., the effect of the treatments was annulled. The same result was obtained by flow 
cytometry. On the other hand, an altered population of oxidized cells by 0.5 mM TBPH was 
detected in hypoosmotic 93 mM buffer based on their forward scatter (FSC) (Figure 1) and 
side scatter (SSC) parameters. The subtle changes in the erythrocyte’s subpopulation 
mechanobiology are essential to monitoring even in healthy people exposed to physical 
or/and environmental stress and stored erythrocytes, but some additional techniques are 
needed for the established optical-based approaches.
PB  - Belgrade: Institute of Physics
C3  - 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts
T1  - Optical methodologies in the analysis of erythrocyte deformability and heterogeneity
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1405
ER  - 
@conference{
author = "Drvenica, Ivana and Trivanović, Drenka and Radmilović, Mihajlo and Vučetić, Dušan and Krmpot, Aleksandar and Ilić, Vesna",
year = "2023",
abstract = "Ektacytometry, a diffraction-based method, measures the deformability of entire erythrocyte populations and does not provide information on the altered deformability of individual erythrocytes or affected subpopulation induced by constant oxidative stress, physical stress, metabolic depletion, and loss of ion gradients during their 120 days life span. We introduced an approach based on ektacytometry coupled to flow cytometry analysis to monitor the deformability and heterogeneity of subpopulations of erythrocytes. The effects of in vitro changes of osmotic gradient (from 155 mM to 93 mM phosphate buffer) and treatment by oxidative agent (0.5 mM and 0.75 mM terc-bytil hydroperoxide (TBPH)) on human erythrocyte isolated from healthy male donors, were tested using RheoScan D 300 (RheoMeditech. Inc., Korea) and BD FACSCalibur flow cytometer (Becton Dickinson, USA).
A decrease in erythrocyte deformability by changes in osmolality or treatment with TBPH 
per se was demonstrated by ektacytometry. Nevertheless, this method could not analyze the 
erythrocytes that underwent both treatments due to their lysis by the shear stress in the 
device. The samples of an equal population of normal erythrocytes and erythrocytes 
rigidified by 0.5 mM TBPH (slightly rigid) showed elongation indices in the physiological 
range, i.e., the effect of the treatments was annulled. The same result was obtained by flow 
cytometry. On the other hand, an altered population of oxidized cells by 0.5 mM TBPH was 
detected in hypoosmotic 93 mM buffer based on their forward scatter (FSC) (Figure 1) and 
side scatter (SSC) parameters. The subtle changes in the erythrocyte’s subpopulation 
mechanobiology are essential to monitoring even in healthy people exposed to physical 
or/and environmental stress and stored erythrocytes, but some additional techniques are 
needed for the established optical-based approaches.",
publisher = "Belgrade: Institute of Physics",
journal = "16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts",
title = "Optical methodologies in the analysis of erythrocyte deformability and heterogeneity",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1405"
}
Drvenica, I., Trivanović, D., Radmilović, M., Vučetić, D., Krmpot, A.,& Ilić, V.. (2023). Optical methodologies in the analysis of erythrocyte deformability and heterogeneity. in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts
Belgrade: Institute of Physics., 27-27.
https://hdl.handle.net/21.15107/rcub_rimi_1405
Drvenica I, Trivanović D, Radmilović M, Vučetić D, Krmpot A, Ilić V. Optical methodologies in the analysis of erythrocyte deformability and heterogeneity. in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts. 2023;:27-27.
https://hdl.handle.net/21.15107/rcub_rimi_1405 .
Drvenica, Ivana, Trivanović, Drenka, Radmilović, Mihajlo, Vučetić, Dušan, Krmpot, Aleksandar, Ilić, Vesna, "Optical methodologies in the analysis of erythrocyte deformability and heterogeneity" in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts (2023):27-27,
https://hdl.handle.net/21.15107/rcub_rimi_1405 .

The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method

Drvenica, Ivana; Mojsilović, Slavko; Stančić, Ana; Marković, Dragana; Kovačić, Marijana; Maslovarić, Irina; Rapajić, Ivana; Vučetić, Dušan; Ilić, Vesna

(Springer Nature, 2021)

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Stančić, Ana
AU  - Marković, Dragana
AU  - Kovačić, Marijana
AU  - Maslovarić, Irina
AU  - Rapajić, Ivana
AU  - Vučetić, Dušan
AU  - Ilić, Vesna
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1176
AB  - Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.
PB  - Springer Nature
T2  - European Biophysics Journal
T1  - The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method
EP  - 846
IS  - 6
SP  - 829
VL  - 50
DO  - 10.1007/s00249-021-01527-3
ER  - 
@article{
author = "Drvenica, Ivana and Mojsilović, Slavko and Stančić, Ana and Marković, Dragana and Kovačić, Marijana and Maslovarić, Irina and Rapajić, Ivana and Vučetić, Dušan and Ilić, Vesna",
year = "2021",
abstract = "Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.",
publisher = "Springer Nature",
journal = "European Biophysics Journal",
title = "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method",
pages = "846-829",
number = "6",
volume = "50",
doi = "10.1007/s00249-021-01527-3"
}
Drvenica, I., Mojsilović, S., Stančić, A., Marković, D., Kovačić, M., Maslovarić, I., Rapajić, I., Vučetić, D.,& Ilić, V.. (2021). The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal
Springer Nature., 50(6), 829-846.
https://doi.org/10.1007/s00249-021-01527-3
Drvenica I, Mojsilović S, Stančić A, Marković D, Kovačić M, Maslovarić I, Rapajić I, Vučetić D, Ilić V. The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal. 2021;50(6):829-846.
doi:10.1007/s00249-021-01527-3 .
Drvenica, Ivana, Mojsilović, Slavko, Stančić, Ana, Marković, Dragana, Kovačić, Marijana, Maslovarić, Irina, Rapajić, Ivana, Vučetić, Dušan, Ilić, Vesna, "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method" in European Biophysics Journal, 50, no. 6 (2021):829-846,
https://doi.org/10.1007/s00249-021-01527-3 . .

Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates

Vučetić, Dušan; Ilić, Vesna; Vojvodić, Danilo; Subota, Vesna; Todorović, Milena; Balint, Bela

(Simti Servizi Srl, Milan, 2018)

TY  - JOUR
AU  - Vučetić, Dušan
AU  - Ilić, Vesna
AU  - Vojvodić, Danilo
AU  - Subota, Vesna
AU  - Todorović, Milena
AU  - Balint, Bela
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/869
AB  - Background. Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations. Materials and methods. Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5. Results. The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected. Discussion. Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation.
PB  - Simti Servizi Srl, Milan
T2  - Blood Transfusion
T1  - Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates
EP  - 92
IS  - 1
SP  - 83
VL  - 16
DO  - 10.2450/2016.0193-16
ER  - 
@article{
author = "Vučetić, Dušan and Ilić, Vesna and Vojvodić, Danilo and Subota, Vesna and Todorović, Milena and Balint, Bela",
year = "2018",
abstract = "Background. Early detection of the platelet storage lesion is still a challenge in transfusion practice. Using flow cytometry, we evaluated the appearance of the storage lesion, based on the expression of platelet activation markers, in total platelets and platelet populations. Materials and methods. Buffy-coat-derived platelet concentrates were stored under standard conditions for 5 days. The expression of activation antigens CD42b, CD36, CD62p and phosphatidylserine on total platelets and populations of small, medium-sized and large platelets was analysed by flow cytometry on storage days 1, 3 and 5. Results. The activation/lesion on total platelets and each platelet population was detected on storage day 3, by the increased expression of CD36. On the same day, increased expression of CD42b and CD62p was detected, but only on large platelets. Small and medium-sized platelets had increased CD62p expression only on day 5. Externalisation of phosphatidylserine was not detected. Discussion. Evaluation of the level of expression of various activation markers on different platelet populations could be an additional valid analysis in cell quality control of platelet concentrates, and in the assessment of novel approaches to platelet concentrate manipulation.",
publisher = "Simti Servizi Srl, Milan",
journal = "Blood Transfusion",
title = "Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates",
pages = "92-83",
number = "1",
volume = "16",
doi = "10.2450/2016.0193-16"
}
Vučetić, D., Ilić, V., Vojvodić, D., Subota, V., Todorović, M.,& Balint, B.. (2018). Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates. in Blood Transfusion
Simti Servizi Srl, Milan., 16(1), 83-92.
https://doi.org/10.2450/2016.0193-16
Vučetić D, Ilić V, Vojvodić D, Subota V, Todorović M, Balint B. Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates. in Blood Transfusion. 2018;16(1):83-92.
doi:10.2450/2016.0193-16 .
Vučetić, Dušan, Ilić, Vesna, Vojvodić, Danilo, Subota, Vesna, Todorović, Milena, Balint, Bela, "Flow cytometry analysis of platelet populations: usefulness for monitoring the storage lesion in pooled buffy-coat platelet concentrates" in Blood Transfusion, 16, no. 1 (2018):83-92,
https://doi.org/10.2450/2016.0193-16 . .
16
6
16

Blood donors' positivity for transfusion-transmissible infections: the Serbian Military Medical Academy experience

Vučetić, Dušan; Kecman, Gorica; Ilić, Vesna; Balint, Bela

(Simiti Servizi Srl, Milan, 2015)

TY  - JOUR
AU  - Vučetić, Dušan
AU  - Kecman, Gorica
AU  - Ilić, Vesna
AU  - Balint, Bela
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/647
AB  - Background. Members of armed forces worldwide are considered to be very susceptible to sexually transmitted infections, thus falling into a high-risk group of blood donors regarding transfusion-transmissible infections. In the Serbian Military Medical Academy a significant number (44% for the period 2005-2013) of blood donations were from members of the Serbian Army. The aim of this study was to determine the significance of military blood donors for the safety of blood transfusion. Material and methods. Between January 2005 and December 2013, a total of 155,479 blood donations were tested for hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV) and syphilis using serological assays (enzyme immunoassays, chemiluminescent microparticle immunoassay and western blot) and molecular testing (polymerase chain reaction analysis). Results. The percentage of blood donations positive for transfusion-transmissible infections in the estimated period was 0.38%, and the percentage of HBV, HCV, HIV and syphilis positive blood donations was 0.20%, 0.12%, 0.005% and 0.06%, respectively. During that period, the percentage of all transfusion-transmissible infections, and in particular of HBV and HCV, declined significantly. In contrast, the percentage of HIV and syphilis positive blood donations remained unchanged. Higher rates of positivity for transfusion-transmissible infections in blood donations from members of the Serbian Army were not found, especially after mandatory military service was abolished in 2009. Discussion. The reported rate of positivity for transfusion-transmissible infections in blood donations from the Military Medical Academy was considered low. This information is of great significance for further implementation of public health measures.
PB  - Simiti Servizi Srl, Milan
T2  - Blood Transfusion
T1  - Blood donors' positivity for transfusion-transmissible infections: the Serbian Military Medical Academy experience
EP  - 575
IS  - 4
SP  - 569
VL  - 13
DO  - 10.2450/2015.0314-14
ER  - 
@article{
author = "Vučetić, Dušan and Kecman, Gorica and Ilić, Vesna and Balint, Bela",
year = "2015",
abstract = "Background. Members of armed forces worldwide are considered to be very susceptible to sexually transmitted infections, thus falling into a high-risk group of blood donors regarding transfusion-transmissible infections. In the Serbian Military Medical Academy a significant number (44% for the period 2005-2013) of blood donations were from members of the Serbian Army. The aim of this study was to determine the significance of military blood donors for the safety of blood transfusion. Material and methods. Between January 2005 and December 2013, a total of 155,479 blood donations were tested for hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV) and syphilis using serological assays (enzyme immunoassays, chemiluminescent microparticle immunoassay and western blot) and molecular testing (polymerase chain reaction analysis). Results. The percentage of blood donations positive for transfusion-transmissible infections in the estimated period was 0.38%, and the percentage of HBV, HCV, HIV and syphilis positive blood donations was 0.20%, 0.12%, 0.005% and 0.06%, respectively. During that period, the percentage of all transfusion-transmissible infections, and in particular of HBV and HCV, declined significantly. In contrast, the percentage of HIV and syphilis positive blood donations remained unchanged. Higher rates of positivity for transfusion-transmissible infections in blood donations from members of the Serbian Army were not found, especially after mandatory military service was abolished in 2009. Discussion. The reported rate of positivity for transfusion-transmissible infections in blood donations from the Military Medical Academy was considered low. This information is of great significance for further implementation of public health measures.",
publisher = "Simiti Servizi Srl, Milan",
journal = "Blood Transfusion",
title = "Blood donors' positivity for transfusion-transmissible infections: the Serbian Military Medical Academy experience",
pages = "575-569",
number = "4",
volume = "13",
doi = "10.2450/2015.0314-14"
}
Vučetić, D., Kecman, G., Ilić, V.,& Balint, B.. (2015). Blood donors' positivity for transfusion-transmissible infections: the Serbian Military Medical Academy experience. in Blood Transfusion
Simiti Servizi Srl, Milan., 13(4), 569-575.
https://doi.org/10.2450/2015.0314-14
Vučetić D, Kecman G, Ilić V, Balint B. Blood donors' positivity for transfusion-transmissible infections: the Serbian Military Medical Academy experience. in Blood Transfusion. 2015;13(4):569-575.
doi:10.2450/2015.0314-14 .
Vučetić, Dušan, Kecman, Gorica, Ilić, Vesna, Balint, Bela, "Blood donors' positivity for transfusion-transmissible infections: the Serbian Military Medical Academy experience" in Blood Transfusion, 13, no. 4 (2015):569-575,
https://doi.org/10.2450/2015.0314-14 . .
4
4
5

Therapeutic use of immunoglobulins

Balint, Bela; Ilić, V.; Taseski, Jovan; Andrić, Z.; Vučetić, DušanVučetić, Dušan

(Vojnomedicinska akademija - Institut za naučne informacije, Beograd, 1999)

TY  - JOUR
AU  - Balint, Bela
AU  - Ilić, V.
AU  - Taseski, Jovan
AU  - Andrić, Z.
AU  - Vučetić, DušanVučetić, Dušan
PY  - 1999
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/55
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Therapeutic use of immunoglobulins
T1  - Terapijska primena imunoglobulina
EP  - 314
IS  - 3
SP  - 303
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_rimi_55
ER  - 
@article{
author = "Balint, Bela and Ilić, V. and Taseski, Jovan and Andrić, Z. and Vučetić, DušanVučetić, Dušan",
year = "1999",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Therapeutic use of immunoglobulins, Terapijska primena imunoglobulina",
pages = "314-303",
number = "3",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_rimi_55"
}
Balint, B., Ilić, V., Taseski, J., Andrić, Z.,& Vučetić, D. D.. (1999). Therapeutic use of immunoglobulins. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 56(3), 303-314.
https://hdl.handle.net/21.15107/rcub_rimi_55
Balint B, Ilić V, Taseski J, Andrić Z, Vučetić DD. Therapeutic use of immunoglobulins. in Vojnosanitetski pregled. 1999;56(3):303-314.
https://hdl.handle.net/21.15107/rcub_rimi_55 .
Balint, Bela, Ilić, V., Taseski, Jovan, Andrić, Z., Vučetić, DušanVučetić, Dušan, "Therapeutic use of immunoglobulins" in Vojnosanitetski pregled, 56, no. 3 (1999):303-314,
https://hdl.handle.net/21.15107/rcub_rimi_55 .