TY  - JOUR
AU  - Simin, Stanislav
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Stefanov, Marijana
AU  - Savović, Milan
AU  - Gajić, Bojan
AU  - Lalošević, Vesna
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1477
AB  - The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Microorganisms
T1  - Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans
IS  - 2
SP  - 233
VL  - 12
DO  - 10.3390/microorganisms12020233
ER  - 

@article{
author = "Simin, Stanislav and Tomanović, Snežana and Sukara, Ratko and Stefanov, Marijana and Savović, Milan and Gajić, Bojan and Lalošević, Vesna",
year = "2024",
abstract = "The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Microorganisms",
title = "Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans",
number = "2",
pages = "233",
volume = "12",
doi = "10.3390/microorganisms12020233"
}

Simin, S., Tomanović, S., Sukara, R., Stefanov, M., Savović, M., Gajić, B.,& Lalošević, V.. (2024). Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans. in Microorganisms
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 233.
https://doi.org/10.3390/microorganisms12020233

Simin S, Tomanović S, Sukara R, Stefanov M, Savović M, Gajić B, Lalošević V. Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans. in Microorganisms. 2024;12(2):233.
doi:10.3390/microorganisms12020233 .

Simin, Stanislav, Tomanović, Snežana, Sukara, Ratko, Stefanov, Marijana, Savović, Milan, Gajić, Bojan, Lalošević, Vesna, "Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans" in Microorganisms, 12, no. 2 (2024):233,
https://doi.org/10.3390/microorganisms12020233 . .

TY  - JOUR
AU  - Kuručki, Milica
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Ćirović, Duško
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1334
AB  - Wild canids are globally recognised as hosts and reservoirs of a large number of ecto- and endoparasites. Data that reveal the importance of the grey wolf (Canis lupus L.1758) in the spread of hepatozoonosis are very scarce. There are a large number of different potential host species that can be infected by Hepatozoon canis, but the most common are domestic and wild carnivores, such as dogs, jackals, foxes, and wolves. In this study, the epidemiological significance of the grey wolf as a host for the pathogen was analysed for the first time in Serbia, as well as the genetic variability of H. canis. The presence of H. canis in wolf spleens has been demonstrated using molecular methods. A total of 107 wolf spleen samples from 30 localities in Serbia were analysed. The presence of H. canis was confirmed in 62 (57.94%) individuals from 26 out of 30 localities. According to the analysis, the sampled H. canis sequences were found to be characterised by a certain heterogeneity. Based on five mutated nucleotide sites in the sequences, H. canis could be divided into five sequence types, S1 to S5. The five sequence types can potentially circulate in grey wolf populations as well as among other domestic and wild canids. This study is the first confirmation of the presence of H. canis in grey wolf populations in Serbia. Considering that the role of this vector-borne disease is poorly researched in wild carnivores, it is very important to indicate the role of this species in the circulation of this pathogen in natural ecosystems.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Animals
T2  - Animals
T1  - High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia
IS  - 23
SP  - 3335
VL  - 12
DO  - 10.3390/ani12233335
ER  - 

@article{
author = "Kuručki, Milica and Tomanović, Snežana and Sukara, Ratko and Ćirović, Duško",
year = "2022",
abstract = "Wild canids are globally recognised as hosts and reservoirs of a large number of ecto- and endoparasites. Data that reveal the importance of the grey wolf (Canis lupus L.1758) in the spread of hepatozoonosis are very scarce. There are a large number of different potential host species that can be infected by Hepatozoon canis, but the most common are domestic and wild carnivores, such as dogs, jackals, foxes, and wolves. In this study, the epidemiological significance of the grey wolf as a host for the pathogen was analysed for the first time in Serbia, as well as the genetic variability of H. canis. The presence of H. canis in wolf spleens has been demonstrated using molecular methods. A total of 107 wolf spleen samples from 30 localities in Serbia were analysed. The presence of H. canis was confirmed in 62 (57.94%) individuals from 26 out of 30 localities. According to the analysis, the sampled H. canis sequences were found to be characterised by a certain heterogeneity. Based on five mutated nucleotide sites in the sequences, H. canis could be divided into five sequence types, S1 to S5. The five sequence types can potentially circulate in grey wolf populations as well as among other domestic and wild canids. This study is the first confirmation of the presence of H. canis in grey wolf populations in Serbia. Considering that the role of this vector-borne disease is poorly researched in wild carnivores, it is very important to indicate the role of this species in the circulation of this pathogen in natural ecosystems.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Animals, Animals",
title = "High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia",
number = "23",
pages = "3335",
volume = "12",
doi = "10.3390/ani12233335"
}

Kuručki, M., Tomanović, S., Sukara, R.,& Ćirović, D.. (2022). High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia. in Animals
Multidisciplinary Digital Publishing Institute (MDPI)., 12(23), 3335.
https://doi.org/10.3390/ani12233335

Kuručki M, Tomanović S, Sukara R, Ćirović D. High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia. in Animals. 2022;12(23):3335.
doi:10.3390/ani12233335 .

Kuručki, Milica, Tomanović, Snežana, Sukara, Ratko, Ćirović, Duško, "High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia" in Animals, 12, no. 23 (2022):3335,
https://doi.org/10.3390/ani12233335 . .

TY  - CONF
AU  - Radmilović, Mihajlo
AU  - Drvenica, Ivana
AU  - Rabasović, Mihailo
AU  - Ilić, Vesna
AU  - Pavlović, Danica
AU  - Oasa, Sho
AU  - Vukojević, Vladana
AU  - Perić, Mina
AU  - Krmpot, Aleksandar
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1439
AB  - Oslikavanje eritrocita bez obeležavanja pomoću konfokalne ili epifluorescentne mikroskopije je praktično nemoguće jer se fluorescencija od hemoglobina (Hb) ne može detektovati zbog brzih neradijativnih raspada koji su dominantni u odnosu na spontanu emisiju. Sa druge strane, demonstrirana je značajna dvofotonska apsorptivnost Hb u infracrvenoj oblasti1 kao i dvofotonski pobuđena fluorescencija (TPEF) od novoformiranog fotoprodukta2 nakon interakcije ultrakratkih laserskih impulsa sa Hb. TPEF mikroskopija je korišćena u brojnim studijama za oslikavanje Hb i eritrocita bez obeležavanja.3 U ovom radu prikazane su fotofizičke osobine i potencijalne primene novoformiranog fotoprodukta. Kreirane su fluorescentne mikrostrukture pomoću direktnog laserskog upisivanja na tankim Hb slojevima (micropatterning). Fotoprodukt je okarakterisan pomoću različitih (mikro)spektroskopskih tehnika: emisije nakon vofotonske apsorpcije, UV-VIS apsorpcije i spektralnog oslikavanja. Razmatrane su spektroskopske osobine protoporfirina IX (PpIX) pošto je on strukturni deo Hb i potencijalno može biti prekursor za formiranje fotoprodukta. Takođe, Hb je tretiran vodonik peroksidom da bi se uočila optička i spektroskopska sličnost tako dobijenog jedinjenja i fotoprodukta. Potvrđena je dugovremena fotostabilnost fotoprodukta u periodima od nekoliko meseci. Na osnovu toga što je fotoprodukt moguće dobiti na prostorno i vremenski kontrolisan način, izvršeno je fotoobeležavanje i praćenje kretanja pojedinačnih eritrocita u humanoj punoj krvi zdravog donora.
PB  - Beograd: Srpsko biološko društvo
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija
T1  - Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita
EP  - 19
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1439
ER  - 

@conference{
author = "Radmilović, Mihajlo and Drvenica, Ivana and Rabasović, Mihailo and Ilić, Vesna and Pavlović, Danica and Oasa, Sho and Vukojević, Vladana and Perić, Mina and Krmpot, Aleksandar",
year = "2022",
abstract = "Oslikavanje eritrocita bez obeležavanja pomoću konfokalne ili epifluorescentne mikroskopije je praktično nemoguće jer se fluorescencija od hemoglobina (Hb) ne može detektovati zbog brzih neradijativnih raspada koji su dominantni u odnosu na spontanu emisiju. Sa druge strane, demonstrirana je značajna dvofotonska apsorptivnost Hb u infracrvenoj oblasti1 kao i dvofotonski pobuđena fluorescencija (TPEF) od novoformiranog fotoprodukta2 nakon interakcije ultrakratkih laserskih impulsa sa Hb. TPEF mikroskopija je korišćena u brojnim studijama za oslikavanje Hb i eritrocita bez obeležavanja.3 U ovom radu prikazane su fotofizičke osobine i potencijalne primene novoformiranog fotoprodukta. Kreirane su fluorescentne mikrostrukture pomoću direktnog laserskog upisivanja na tankim Hb slojevima (micropatterning). Fotoprodukt je okarakterisan pomoću različitih (mikro)spektroskopskih tehnika: emisije nakon vofotonske apsorpcije, UV-VIS apsorpcije i spektralnog oslikavanja. Razmatrane su spektroskopske osobine protoporfirina IX (PpIX) pošto je on strukturni deo Hb i potencijalno može biti prekursor za formiranje fotoprodukta. Takođe, Hb je tretiran vodonik peroksidom da bi se uočila optička i spektroskopska sličnost tako dobijenog jedinjenja i fotoprodukta. Potvrđena je dugovremena fotostabilnost fotoprodukta u periodima od nekoliko meseci. Na osnovu toga što je fotoprodukt moguće dobiti na prostorno i vremenski kontrolisan način, izvršeno je fotoobeležavanje i praćenje kretanja pojedinačnih eritrocita u humanoj punoj krvi zdravog donora.",
publisher = "Beograd: Srpsko biološko društvo",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija",
title = "Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1439"
}

Radmilović, M., Drvenica, I., Rabasović, M., Ilić, V., Pavlović, D., Oasa, S., Vukojević, V., Perić, M.,& Krmpot, A.. (2022). Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija
Beograd: Srpsko biološko društvo., 19-19.
https://hdl.handle.net/21.15107/rcub_rimi_1439

Radmilović M, Drvenica I, Rabasović M, Ilić V, Pavlović D, Oasa S, Vukojević V, Perić M, Krmpot A. Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija. 2022;:19-19.
https://hdl.handle.net/21.15107/rcub_rimi_1439 .

Radmilović, Mihajlo, Drvenica, Ivana, Rabasović, Mihailo, Ilić, Vesna, Pavlović, Danica, Oasa, Sho, Vukojević, Vladana, Perić, Mina, Krmpot, Aleksandar, "Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija (2022):19-19,
https://hdl.handle.net/21.15107/rcub_rimi_1439 .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Repac, Jelena
AU  - Božić, Bojan
AU  - Radulović, Željko
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Ristanović, Elizabeta
AU  - Chochlakis, Dimosthenis
AU  - Nedeljković Božić, Biljana 
AU  - Tomanović, Snežana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1160
AB  - In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.
PB  - Springer
T2  - Experimental and Applied Acarology
T1  - Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization
EP  - 99
IS  - 1
SP  - 83
VL  - 85
DO  - 10.1007/s10493-021-00653-z
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Repac, Jelena and Božić, Bojan and Radulović, Željko and Veinović, Gorana and Sukara, Ratko and Ristanović, Elizabeta and Chochlakis, Dimosthenis and Nedeljković Božić, Biljana  and Tomanović, Snežana",
year = "2021",
abstract = "In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.",
publisher = "Springer",
journal = "Experimental and Applied Acarology",
title = "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization",
pages = "99-83",
number = "1",
volume = "85",
doi = "10.1007/s10493-021-00653-z"
}

Mihaljica, D., Marković, D., Repac, J., Božić, B., Radulović, Ž., Veinović, G., Sukara, R., Ristanović, E., Chochlakis, D., Nedeljković Božić, B.,& Tomanović, S.. (2021). Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology
Springer., 85(1), 83-99.
https://doi.org/10.1007/s10493-021-00653-z

Mihaljica D, Marković D, Repac J, Božić B, Radulović Ž, Veinović G, Sukara R, Ristanović E, Chochlakis D, Nedeljković Božić B, Tomanović S. Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology. 2021;85(1):83-99.
doi:10.1007/s10493-021-00653-z .

Mihaljica, Darko, Marković, Dragana, Repac, Jelena, Božić, Bojan, Radulović, Željko, Veinović, Gorana, Sukara, Ratko, Ristanović, Elizabeta, Chochlakis, Dimosthenis, Nedeljković Božić, Biljana , Tomanović, Snežana, "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization" in Experimental and Applied Acarology, 85, no. 1 (2021):83-99,
https://doi.org/10.1007/s10493-021-00653-z . .

TY  - CONF
AU  - Repac, Jelena
AU  - Mihaljica, Darko
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
AU  - Tomanović, Snežana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1416
PB  - Belgrade:Serbian Society for Parasitology
C3  - 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts
T1  - In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity
EP  - 112
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1416
ER  - 

@conference{
author = "Repac, Jelena and Mihaljica, Darko and Božić, Bojan and Božić Nedeljković, Biljana and Tomanović, Snežana",
year = "2021",
publisher = "Belgrade:Serbian Society for Parasitology",
journal = "13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts",
title = "In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity",
pages = "112-112",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1416"
}

Repac, J., Mihaljica, D., Božić, B., Božić Nedeljković, B.,& Tomanović, S.. (2021). In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity. in 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts
Belgrade:Serbian Society for Parasitology., 112-112.
https://hdl.handle.net/21.15107/rcub_rimi_1416

Repac J, Mihaljica D, Božić B, Božić Nedeljković B, Tomanović S. In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity. in 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts. 2021;:112-112.
https://hdl.handle.net/21.15107/rcub_rimi_1416 .

Repac, Jelena, Mihaljica, Darko, Božić, Bojan, Božić Nedeljković, Biljana, Tomanović, Snežana, "In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity" in 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts (2021):112-112,
https://hdl.handle.net/21.15107/rcub_rimi_1416 .

TY  - JOUR
AU  - Dakić, Tamara
AU  - Lakić, Iva
AU  - Zec, Manja
AU  - Takić, Marija
AU  - Stojiljković, Mojca
AU  - Jevđović, Tanja
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1197
AB  - BACKGROUND: Nutritional modulations may be considered a strategy to protect mental health. Neuronal homeostasis is highly dependent on the availability of glucose, which represents the primary energy source for the brain. In this study, we evaluated the effects of walnut intake and fructose-rich diet on the expression of glucose transporters (GLUTs) in two rat brain regions: hypothalamus and hippocampus. RESULTS: Our results show that walnut supplementation of fructose-fed animals restored the hypothalamic content of GLUT1 and GLUT3 protein. Furthermore, walnut intake did not affect increased hypothalamic GLUT2 content upon fructose consumption. These effects were accompanied by distinctive alterations of hippocampal GLUTs levels. Specifically, walnut intake increased GLUT1 content, whereas GLUT2 protein was decreased within the rat hippocampus after both individual and combined treatments. CONCLUSION: Overall, our study suggests that walnut supplementation exerted modulatory effects on the glucose transporters within specific brain regions in the presence of developed metabolic disorder.
PB  - Wiley
T2  - Journal of the Science of Food and Agriculture
T1  - Fructose-rich diet and walnut supplementation differently regulate rat hypothalamic and hippocampal glucose transporters expression
EP  - 5991
IS  - 14
SP  - 5984
VL  - 101
DO  - 10.1002/jsfa.11252
ER  - 

@article{
author = "Dakić, Tamara and Lakić, Iva and Zec, Manja and Takić, Marija and Stojiljković, Mojca and Jevđović, Tanja",
year = "2021",
abstract = "BACKGROUND: Nutritional modulations may be considered a strategy to protect mental health. Neuronal homeostasis is highly dependent on the availability of glucose, which represents the primary energy source for the brain. In this study, we evaluated the effects of walnut intake and fructose-rich diet on the expression of glucose transporters (GLUTs) in two rat brain regions: hypothalamus and hippocampus. RESULTS: Our results show that walnut supplementation of fructose-fed animals restored the hypothalamic content of GLUT1 and GLUT3 protein. Furthermore, walnut intake did not affect increased hypothalamic GLUT2 content upon fructose consumption. These effects were accompanied by distinctive alterations of hippocampal GLUTs levels. Specifically, walnut intake increased GLUT1 content, whereas GLUT2 protein was decreased within the rat hippocampus after both individual and combined treatments. CONCLUSION: Overall, our study suggests that walnut supplementation exerted modulatory effects on the glucose transporters within specific brain regions in the presence of developed metabolic disorder.",
publisher = "Wiley",
journal = "Journal of the Science of Food and Agriculture",
title = "Fructose-rich diet and walnut supplementation differently regulate rat hypothalamic and hippocampal glucose transporters expression",
pages = "5991-5984",
number = "14",
volume = "101",
doi = "10.1002/jsfa.11252"
}

Dakić, T., Lakić, I., Zec, M., Takić, M., Stojiljković, M.,& Jevđović, T.. (2021). Fructose-rich diet and walnut supplementation differently regulate rat hypothalamic and hippocampal glucose transporters expression. in Journal of the Science of Food and Agriculture
Wiley., 101(14), 5984-5991.
https://doi.org/10.1002/jsfa.11252

Dakić T, Lakić I, Zec M, Takić M, Stojiljković M, Jevđović T. Fructose-rich diet and walnut supplementation differently regulate rat hypothalamic and hippocampal glucose transporters expression. in Journal of the Science of Food and Agriculture. 2021;101(14):5984-5991.
doi:10.1002/jsfa.11252 .

Dakić, Tamara, Lakić, Iva, Zec, Manja, Takić, Marija, Stojiljković, Mojca, Jevđović, Tanja, "Fructose-rich diet and walnut supplementation differently regulate rat hypothalamic and hippocampal glucose transporters expression" in Journal of the Science of Food and Agriculture, 101, no. 14 (2021):5984-5991,
https://doi.org/10.1002/jsfa.11252 . .

TY  - JOUR
AU  - Kočović, Dušica M.
AU  - Bajuk-Bogdanović, Danica
AU  - Maslovarić, Irina
AU  - Božić-Nedeljković, Biljana
AU  - Anđus, Pavle R.
AU  - Daković, Marko
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1157
AB  - The search for an effective and non-toxic radioprotector is ongoing. We tested a novel, natural aminothiol-based radioprotector, GL2011, that was applied 30 min, 3 h or 6 h after the exposure of male albino Wistar rats to a 6.7 Gy mild dose of gamma radiation. The molecular signatures of radioprotection were investigated with Raman microspectroscopy of brainstem tissue samples. Morphological changes and activation of astrocytes and microglia were assessed by immunohistochemistry. Global markers of neuroinflammation were followed by ELISA to monitor blood plasma levels of proinflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines. A thirty-day follow-up determined survival of unprotected animals 37.5%. A survival increase was observed after radioprotection (75%, irrespective of the time of application). Raman spectra revealed a slightly deleterious effect of radiation on nucleic acids in surviving animals that was mitigated with the radioprotector, as GL2011 preserved the morphology of both astrocytes and microglia, with reduced microglial infiltration. Cytokine assessment revealed an immunomodulatory effect of the novel radioprotector. The overall results point out the positive effects of a single dose of GL2011 applied at different times. The molecular and cellular changes in the brainstem indicate that the radioprotector applied after radiation conferred better protection, which underlines its translation to cure radiation accidents.
PB  - Serbian Biological Society, Institute for Biological Research "Siniša Stanković", Faculty of Biology, University of Belgrade, Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Institute for the Application of Nuclear Energy
T2  - Archives of Biological Sciences
T1  - Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011
EP  - 173
IS  - 2
SP  - 161
VL  - 73
DO  - 10.2298/ABS210129011K
ER  - 

@article{
author = "Kočović, Dušica M. and Bajuk-Bogdanović, Danica and Maslovarić, Irina and Božić-Nedeljković, Biljana and Anđus, Pavle R. and Daković, Marko",
year = "2021",
abstract = "The search for an effective and non-toxic radioprotector is ongoing. We tested a novel, natural aminothiol-based radioprotector, GL2011, that was applied 30 min, 3 h or 6 h after the exposure of male albino Wistar rats to a 6.7 Gy mild dose of gamma radiation. The molecular signatures of radioprotection were investigated with Raman microspectroscopy of brainstem tissue samples. Morphological changes and activation of astrocytes and microglia were assessed by immunohistochemistry. Global markers of neuroinflammation were followed by ELISA to monitor blood plasma levels of proinflammatory (IL-6 and TNF-α) and anti-inflammatory (IL-10) cytokines. A thirty-day follow-up determined survival of unprotected animals 37.5%. A survival increase was observed after radioprotection (75%, irrespective of the time of application). Raman spectra revealed a slightly deleterious effect of radiation on nucleic acids in surviving animals that was mitigated with the radioprotector, as GL2011 preserved the morphology of both astrocytes and microglia, with reduced microglial infiltration. Cytokine assessment revealed an immunomodulatory effect of the novel radioprotector. The overall results point out the positive effects of a single dose of GL2011 applied at different times. The molecular and cellular changes in the brainstem indicate that the radioprotector applied after radiation conferred better protection, which underlines its translation to cure radiation accidents.",
publisher = "Serbian Biological Society, Institute for Biological Research "Siniša Stanković", Faculty of Biology, University of Belgrade, Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Institute for the Application of Nuclear Energy",
journal = "Archives of Biological Sciences",
title = "Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011",
pages = "173-161",
number = "2",
volume = "73",
doi = "10.2298/ABS210129011K"
}

Kočović, D. M., Bajuk-Bogdanović, D., Maslovarić, I., Božić-Nedeljković, B., Anđus, P. R.,& Daković, M.. (2021). Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011. in Archives of Biological Sciences
Serbian Biological Society, Institute for Biological Research "Siniša Stanković", Faculty of Biology, University of Belgrade, Department of Biology and Ecology, Faculty of Science, University of Novi Sad, Institute for the Application of Nuclear Energy., 73(2), 161-173.
https://doi.org/10.2298/ABS210129011K

Kočović DM, Bajuk-Bogdanović D, Maslovarić I, Božić-Nedeljković B, Anđus PR, Daković M. Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011. in Archives of Biological Sciences. 2021;73(2):161-173.
doi:10.2298/ABS210129011K .

Kočović, Dušica M., Bajuk-Bogdanović, Danica, Maslovarić, Irina, Božić-Nedeljković, Biljana, Anđus, Pavle R., Daković, Marko, "Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011" in Archives of Biological Sciences, 73, no. 2 (2021):161-173,
https://doi.org/10.2298/ABS210129011K . .