TY  - JOUR
AU  - Šefer, Dijana
AU  - Miljić, Predrag
AU  - Kraguljac-Kurtović, Nada
AU  - Bižić-Radulović, Sandra
AU  - Bogdanović, Andrija
AU  - Knežević, Vesna
AU  - Marković, Dragana
AU  - Beleslin-Čokić, Bojana
AU  - Novaković, Ivana
AU  - Marinković, Jelena
AU  - Leković, Danijela
AU  - Gotić, Mirjana
AU  - Čokić, Vladan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1231
AB  - Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA.
T2  - International Journal of Laboratory Hematology
T1  - Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms
EP  - 312
IS  - 2
SP  - 302
VL  - 44
DO  - 10.1111/ijlh.13754
ER  - 

@article{
author = "Šefer, Dijana and Miljić, Predrag and Kraguljac-Kurtović, Nada and Bižić-Radulović, Sandra and Bogdanović, Andrija and Knežević, Vesna and Marković, Dragana and Beleslin-Čokić, Bojana and Novaković, Ivana and Marinković, Jelena and Leković, Danijela and Gotić, Mirjana and Čokić, Vladan",
year = "2022",
abstract = "Introduction: The impact of activated blood and endothelial cells on the thrombosis in myeloproliferative neoplasms (MPN) has not yet been clarified. We prospectively analyzed correlation between circulating leukocyte-platelet aggregates and soluble selectins to thrombosis occurrence in MPN, in the context of standard and cardiovascular risk factors, and different clinical and biological characteristics. Methods: Flow cytometric analysis of neutrophil-platelet (Neu-Plt) and monocyte-platelet (Mo-Plt) aggregates in peripheral blood, as well as quantification of soluble E-/L-/P-selectins by enzyme immunoassay, was performed on 95 newly diagnosed MPN patients. Results: During the follow-up, thrombosis occurred in 12.6% MPN patients (arterial 9.4%, venous 3.2%), with a mean time of 39 months. The overall incidence rate of main thrombotic events was 4.36 per 100 patient-years. The incidence of arterial hypertension (HTA) was significantly higher in patients with thrombosis, compared to those without thrombosis (P <.05). The level of soluble P-selectin was significantly higher in patients with thrombosis compared to those without thrombosis (346.89 ng/mL vs 286.39 ng/mL, P =.034). The mean level of Neu-Plt (26.7% vs 22.4%) and Mo-Plt (17.8% vs 12.3%) aggregates did not differ significantly between the groups with and without thrombosis. A multivariate COX proportional hazard regression model confirmed an independent predictive significance of Mo-Plt aggregates (HR = 1.561, 95% CI: 1.007-2.420, P =.046), as well as the cumulative effect of Mo-Plt aggregates and HTA (HR = 1.975, 95%CI: 1.215-3.212, P =.006) for thrombosis occurrence. Conclusion: Monocyte-platelet aggregates represent an independent risk factor for thrombosis occurrence, further on supported by HTA.",
journal = "International Journal of Laboratory Hematology",
title = "Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms",
pages = "312-302",
number = "2",
volume = "44",
doi = "10.1111/ijlh.13754"
}

Šefer, D., Miljić, P., Kraguljac-Kurtović, N., Bižić-Radulović, S., Bogdanović, A., Knežević, V., Marković, D., Beleslin-Čokić, B., Novaković, I., Marinković, J., Leković, D., Gotić, M.,& Čokić, V.. (2022). Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms. in International Journal of Laboratory Hematology, 44(2), 302-312.
https://doi.org/10.1111/ijlh.13754

Šefer D, Miljić P, Kraguljac-Kurtović N, Bižić-Radulović S, Bogdanović A, Knežević V, Marković D, Beleslin-Čokić B, Novaković I, Marinković J, Leković D, Gotić M, Čokić V. Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms. in International Journal of Laboratory Hematology. 2022;44(2):302-312.
doi:10.1111/ijlh.13754 .

Šefer, Dijana, Miljić, Predrag, Kraguljac-Kurtović, Nada, Bižić-Radulović, Sandra, Bogdanović, Andrija, Knežević, Vesna, Marković, Dragana, Beleslin-Čokić, Bojana, Novaković, Ivana, Marinković, Jelena, Leković, Danijela, Gotić, Mirjana, Čokić, Vladan, "Correlation between leukocyte-platelet aggregates and thrombosis in myeloproliferative neoplasms" in International Journal of Laboratory Hematology, 44, no. 2 (2022):302-312,
https://doi.org/10.1111/ijlh.13754 . .