TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Grujić-Milanović, Jelica
AU  - Vejnović, Branislav
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1237
AB  - Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.
PB  - Taylor & Francis
T2  - Italian Journal of Animal Science
T1  - Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows
EP  - 858
IS  - 1
SP  - 847
VL  - 21
DO  - 10.1080/1828051X.2022.2070034
ER  - 

@article{
author = "Drvenica, Ivana and Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Grujić-Milanović, Jelica and Vejnović, Branislav and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna",
year = "2022",
abstract = "Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.",
publisher = "Taylor & Francis",
journal = "Italian Journal of Animal Science",
title = "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows",
pages = "858-847",
number = "1",
volume = "21",
doi = "10.1080/1828051X.2022.2070034"
}

Drvenica, I., Stojić, M., Fratrić, N., Kovačić, M., Grujić-Milanović, J., Vejnović, B., Marković, D., Gvozdić, D.,& Ilić, V.. (2022). Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science
Taylor & Francis., 21(1), 847-858.
https://doi.org/10.1080/1828051X.2022.2070034

Drvenica I, Stojić M, Fratrić N, Kovačić M, Grujić-Milanović J, Vejnović B, Marković D, Gvozdić D, Ilić V. Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science. 2022;21(1):847-858.
doi:10.1080/1828051X.2022.2070034 .

Drvenica, Ivana, Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Grujić-Milanović, Jelica, Vejnović, Branislav, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows" in Italian Journal of Animal Science, 21, no. 1 (2022):847-858,
https://doi.org/10.1080/1828051X.2022.2070034 . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Fratrić, Natalija
AU  - Arsić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Drvenica, Ivana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Ilić, Vesna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1010
AB  - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
EP  - 74
SP  - 63
VL  - 133
DO  - 10.1016/j.rvsc.2020.09.004
ER  - 

@article{
author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić-Milanović, Jelica and Ivanov, Milan and Ilić, Vesna",
year = "2020",
abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes",
pages = "74-63",
volume = "133",
doi = "10.1016/j.rvsc.2020.09.004"
}

Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić-Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 133, 63-74.
https://doi.org/10.1016/j.rvsc.2020.09.004

Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić-Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74.
doi:10.1016/j.rvsc.2020.09.004 .

Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić-Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74,
https://doi.org/10.1016/j.rvsc.2020.09.004 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Ilić, Vesna
AU  - Kovačić, Marijana
AU  - Gvozdić, Dragan
AU  - Stajković, Silvana
AU  - Vejnović, Branislav
AU  - Savić, Olivera
AU  - Fratrić, Natalija
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1017
AB  - This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows
EP  - 435
IS  - 4
SP  - 429
VL  - 87
DO  - 10.1017/S0022029920001077
ER  - 

@article{
author = "Stojić, Milica and Ilić, Vesna and Kovačić, Marijana and Gvozdić, Dragan and Stajković, Silvana and Vejnović, Branislav and Savić, Olivera and Fratrić, Natalija",
year = "2020",
abstract = "This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows",
pages = "435-429",
number = "4",
volume = "87",
doi = "10.1017/S0022029920001077"
}

Stojić, M., Ilić, V., Kovačić, M., Gvozdić, D., Stajković, S., Vejnović, B., Savić, O.,& Fratrić, N.. (2020). Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research
Cambridge Univ Press, New York., 87(4), 429-435.
https://doi.org/10.1017/S0022029920001077

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research. 2020;87(4):429-435.
doi:10.1017/S0022029920001077 .

Stojić, Milica, Ilić, Vesna, Kovačić, Marijana, Gvozdić, Dragan, Stajković, Silvana, Vejnović, Branislav, Savić, Olivera, Fratrić, Natalija, "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows" in Journal of Dairy Research, 87, no. 4 (2020):429-435,
https://doi.org/10.1017/S0022029920001077 . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Obrenović, Sonja
AU  - Grujić-Milanović, Jelica
AU  - Arsić, Aleksandra
AU  - Milanović, Zorana
AU  - Savić, Olivera
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/770
AB  - Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers
EP  - 227
IS  - 2
SP  - 207
VL  - 67
DO  - 10.1515/acve-2017-0018
ER  - 

@article{
author = "Kovačić, Marijana and Marković, Dragana and Maslovarić, Irina and Obrenović, Sonja and Grujić-Milanović, Jelica and Arsić, Aleksandra and Milanović, Zorana and Savić, Olivera and Fratrić, Natalija and Ilić, Vesna",
year = "2017",
abstract = "Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDSPAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and a globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total. globulins (IgG), due to an increased concentration of anionic. globulins (predominately IgG1), was detected. The increased concentration of anionic. globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers",
pages = "227-207",
number = "2",
volume = "67",
doi = "10.1515/acve-2017-0018"
}

Kovačić, M., Marković, D., Maslovarić, I., Obrenović, S., Grujić-Milanović, J., Arsić, A., Milanović, Z., Savić, O., Fratrić, N.,& Ilić, V.. (2017). Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 207-227.
https://doi.org/10.1515/acve-2017-0018

Kovačić M, Marković D, Maslovarić I, Obrenović S, Grujić-Milanović J, Arsić A, Milanović Z, Savić O, Fratrić N, Ilić V. Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers. in Acta Veterinaria-Beograd. 2017;67(2):207-227.
doi:10.1515/acve-2017-0018 .

Kovačić, Marijana, Marković, Dragana, Maslovarić, Irina, Obrenović, Sonja, Grujić-Milanović, Jelica, Arsić, Aleksandra, Milanović, Zorana, Savić, Olivera, Fratrić, Natalija, Ilić, Vesna, "Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers" in Acta Veterinaria-Beograd, 67, no. 2 (2017):207-227,
https://doi.org/10.1515/acve-2017-0018 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valcić, Olivera
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/779
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P lt 0.001 and P lt 0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin lt 10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
EP  - 524
IS  - 4
SP  - 508
VL  - 67
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valcić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P lt 0.001 and P lt 0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin lt 10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
pages = "524-508",
number = "4",
volume = "67",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovačić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valcić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovačić M, Ilić V, Gvozdić D, Savić O, Doković R, Valcić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valcić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Mojsilović, Slavko
AU  - Mišić, Dušan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Valčić, Olivera
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
AU  - Fratrić, Natalija
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/717
AB  - In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation
EP  - 142
SP  - 135
VL  - 106
DO  - 10.1016/j.rvsc.2016.04.002
ER  - 

@article{
author = "Kovačić, Marijana and Mojsilović, Slavko and Mišić, Dušan and Vuković, Dejan and Savić, Olivera and Valčić, Olivera and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna and Fratrić, Natalija",
year = "2016",
abstract = "In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows' granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes' viability. The strongest effect of inhibition of cows' granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves' MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves' MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation",
pages = "142-135",
volume = "106",
doi = "10.1016/j.rvsc.2016.04.002"
}

Kovačić, M., Mojsilović, S., Mišić, D., Vuković, D., Savić, O., Valčić, O., Marković, D., Gvozdić, D., Ilić, V.,& Fratrić, N.. (2016). Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 106, 135-142.
https://doi.org/10.1016/j.rvsc.2016.04.002

Kovačić M, Mojsilović S, Mišić D, Vuković D, Savić O, Valčić O, Marković D, Gvozdić D, Ilić V, Fratrić N. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation. in Research in Veterinary Science. 2016;106:135-142.
doi:10.1016/j.rvsc.2016.04.002 .

Kovačić, Marijana, Mojsilović, Slavko, Mišić, Dušan, Vuković, Dejan, Savić, Olivera, Valčić, Olivera, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, Fratrić, Natalija, "Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation" in Research in Veterinary Science, 106 (2016):135-142,
https://doi.org/10.1016/j.rvsc.2016.04.002 . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/399
AB  - Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology & Immunopathology
T1  - Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG
EP  - 168
IS  - 3-4
SP  - 161
VL  - 150
DO  - 10.1016/j.vetimm.2012.09.009
ER  - 

@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vuković, Dejan and Savić, Olivera and Kovačić, Marijana and Ilić, Vesna",
year = "2012",
abstract = "Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology & Immunopathology",
title = "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG",
pages = "168-161",
number = "3-4",
volume = "150",
doi = "10.1016/j.vetimm.2012.09.009"
}

Fratrić, N., Gvozdić, D., Vuković, D., Savić, O., Kovačić, M.,& Ilić, V.. (2012). Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology
Elsevier, Amsterdam., 150(3-4), 161-168.
https://doi.org/10.1016/j.vetimm.2012.09.009

Fratrić N, Gvozdić D, Vuković D, Savić O, Kovačić M, Ilić V. Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology. 2012;150(3-4):161-168.
doi:10.1016/j.vetimm.2012.09.009 .

Fratrić, Natalija, Gvozdić, Dragan, Vuković, Dejan, Savić, Olivera, Kovačić, Marijana, Ilić, Vesna, "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG" in Veterinary Immunology & Immunopathology, 150, no. 3-4 (2012):161-168,
https://doi.org/10.1016/j.vetimm.2012.09.009 . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Jagličić, Ivana
AU  - Ilić, Vesna
PY  - 2011
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/375
AB  - Pneumonia in preruminant calves is a multifactorial disease. Infectious agents, the environment, management and the immune status of the calves are all important factors in determining the outcome of an infection. Until today, the level and composition of circulating immune complexes in preruminant calves with pneumonia have not been studied in detail. We performed this work with the aim to determine whether pneumonia in three-month-old calves is followed by changes in the immune complex level and changes in the γ-globulin level as their possible constituents. Immune complexes from the calves' sera were isolated by polyethylene glycol (PEG) precipitation methods. Optical density at 350 nm (OD350) of redissolved precipitates was measured to determine the circulating immune complexes level. The OD350 level of PEG precipitates of calves with pneumonia at the time of diagnosis was 0.577±0.206 and it was statistically significantly higher (p lt 0.001) than OD350 the level of PEG precipitates of healthy calves (0.286±0.080). Electrophoretic analysis of sera and PEG precipitates showed that both slow and fast γ-globulins are found among serum and immune-complexes' γ-globulins, but the concentration of fast γ-globulins was significantly lower in sera of diseased calves. The level of PEG precipitable immune complexes was not correlated with the concentration of serum and PEG precipitable g-globulins. The results of this study have shown that by relatively simple PEG precipitation assay it is possible to detect an increased level of circulating immune complexes in calves with pneumonia. This can be used as an additional diagnostic parameter for the detection and follow up of the disease.
AB  - Oboljenja respiratornog trakta goveda su kompleksne etiologije, a javljaju se kao posledica interakcije imunskog sistema jedinke, stresogenih činilaca prisutnih u spoljašnjoj sredini i patogenih mikroorganizama. Nivo i uloga cirkulišućih imunskih kompleksa u serumima teladi obolelih od ove bolesti do sada nisu izučavani. Ovaj rad je urađen sa ciljem da se utvrdi da li su bolesti respiratornog trakta kod teladi u uzrastu od tri meseca praćene promenom nivoa imunskih kompleksa u serumu i promenom nivoa serumskih proteina, pre svega γ globulina, kao osnovnih konstituenata imunskih kompleksa. Imunski kompleksi iz seruma teladi su izolovani precipitacijom polietilen glikolom (PEG-om). Nivo PEG precipitabilnih imunskih kompleksa je određivan merenjem optičke gustine PEG precipitata na 350 nm (OD350). OD350 PEG precipitata obolele teladi je iznosila 0,577±0,206 i bila je statistički značajno viša (p lt 0,001) u odnosu na vrednosti dobijene kod zdrave teladi (OD350 = 0,286±0,080). U γ-globulinskoj frakciji seruma i PEG precipitata i zdrave i obolele teladi bili su prisutni i spori, katjonski, i brzi, anjonski γ-globulini, ali je koncentracija brzih γ-globulina bila značajno niža kod obolele teladi. Nivo ukupnih γ-globulina i γ-globulinskih frakcija nije statistički značajno korelirao sa nivoom PEG precipitabilnih imunskih kompleksa. Na osnovu dobijenih razultata može se zaključiti da se u serumima tri meseca starih teladi obolelih od bolesti respiratornog trakta detektuje povećan nivo PEG precipitabilnih imunskih kompleksa, a rezultati dobijeni ovim relativno jednostavnim testom mogu se koristiti kao dodatni parametar za dijagnozu i praćenje toka bolesti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia
T1  - Imunski kompleksi u krvnom serumu teladi sa kliničkim simptomima bronhopneumonije
EP  - 24
IS  - 1-2
SP  - 13
VL  - 65
DO  - 10.2298/VETGL1102013F
ER  - 

@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Savić, Olivera and Jagličić, Ivana and Ilić, Vesna",
year = "2011",
abstract = "Pneumonia in preruminant calves is a multifactorial disease. Infectious agents, the environment, management and the immune status of the calves are all important factors in determining the outcome of an infection. Until today, the level and composition of circulating immune complexes in preruminant calves with pneumonia have not been studied in detail. We performed this work with the aim to determine whether pneumonia in three-month-old calves is followed by changes in the immune complex level and changes in the γ-globulin level as their possible constituents. Immune complexes from the calves' sera were isolated by polyethylene glycol (PEG) precipitation methods. Optical density at 350 nm (OD350) of redissolved precipitates was measured to determine the circulating immune complexes level. The OD350 level of PEG precipitates of calves with pneumonia at the time of diagnosis was 0.577±0.206 and it was statistically significantly higher (p lt 0.001) than OD350 the level of PEG precipitates of healthy calves (0.286±0.080). Electrophoretic analysis of sera and PEG precipitates showed that both slow and fast γ-globulins are found among serum and immune-complexes' γ-globulins, but the concentration of fast γ-globulins was significantly lower in sera of diseased calves. The level of PEG precipitable immune complexes was not correlated with the concentration of serum and PEG precipitable g-globulins. The results of this study have shown that by relatively simple PEG precipitation assay it is possible to detect an increased level of circulating immune complexes in calves with pneumonia. This can be used as an additional diagnostic parameter for the detection and follow up of the disease., Oboljenja respiratornog trakta goveda su kompleksne etiologije, a javljaju se kao posledica interakcije imunskog sistema jedinke, stresogenih činilaca prisutnih u spoljašnjoj sredini i patogenih mikroorganizama. Nivo i uloga cirkulišućih imunskih kompleksa u serumima teladi obolelih od ove bolesti do sada nisu izučavani. Ovaj rad je urađen sa ciljem da se utvrdi da li su bolesti respiratornog trakta kod teladi u uzrastu od tri meseca praćene promenom nivoa imunskih kompleksa u serumu i promenom nivoa serumskih proteina, pre svega γ globulina, kao osnovnih konstituenata imunskih kompleksa. Imunski kompleksi iz seruma teladi su izolovani precipitacijom polietilen glikolom (PEG-om). Nivo PEG precipitabilnih imunskih kompleksa je određivan merenjem optičke gustine PEG precipitata na 350 nm (OD350). OD350 PEG precipitata obolele teladi je iznosila 0,577±0,206 i bila je statistički značajno viša (p lt 0,001) u odnosu na vrednosti dobijene kod zdrave teladi (OD350 = 0,286±0,080). U γ-globulinskoj frakciji seruma i PEG precipitata i zdrave i obolele teladi bili su prisutni i spori, katjonski, i brzi, anjonski γ-globulini, ali je koncentracija brzih γ-globulina bila značajno niža kod obolele teladi. Nivo ukupnih γ-globulina i γ-globulinskih frakcija nije statistički značajno korelirao sa nivoom PEG precipitabilnih imunskih kompleksa. Na osnovu dobijenih razultata može se zaključiti da se u serumima tri meseca starih teladi obolelih od bolesti respiratornog trakta detektuje povećan nivo PEG precipitabilnih imunskih kompleksa, a rezultati dobijeni ovim relativno jednostavnim testom mogu se koristiti kao dodatni parametar za dijagnozu i praćenje toka bolesti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia, Imunski kompleksi u krvnom serumu teladi sa kliničkim simptomima bronhopneumonije",
pages = "24-13",
number = "1-2",
volume = "65",
doi = "10.2298/VETGL1102013F"
}

Fratrić, N., Gvozdić, D., Savić, O., Jagličić, I.,& Ilić, V.. (2011). Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1-2), 13-24.
https://doi.org/10.2298/VETGL1102013F

Fratrić N, Gvozdić D, Savić O, Jagličić I, Ilić V. Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia. in Veterinarski glasnik. 2011;65(1-2):13-24.
doi:10.2298/VETGL1102013F .

Fratrić, Natalija, Gvozdić, Dragan, Savić, Olivera, Jagličić, Ivana, Ilić, Vesna, "Immune complexes in blood serum of calves with clinical symptoms of bronchopneumonia" in Veterinarski glasnik, 65, no. 1-2 (2011):13-24,
https://doi.org/10.2298/VETGL1102013F . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
AU  - Milošević-Jovčić, Nadežda
AU  - Stojić, V.
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/306
AB  - Preruminant calves encounter numerous antigens, and formation of immune complexes is necessary for antigens elimination. The capability of immunologically immature calves to form immune complexes has not yet been studied in detail. For immune complexes studies, selective precipitation by PEG was performed, in combination with agarose gel electrophoresis and immunoelectrophoresis, with an aim to determine some properties of IgG, as constituents of immune complexes. In our previous work it was shown that the level of PEG precipitable immune complexes increased in the period from birth to 48-hours of life, decreased at day 10, increased in one month old animals, and after that, stayed unchanged until 4th month of age. Electrophoretic and immunoelectrophoretic analysis showed that until one month of age, calves' sera and PEG precipitates contained only one part of IgG molecules which corresponded to fast, anionic γ globulins. Although at the age of one month, preruminant calves' sera contained all molecular forms of IgG molecules present in the sera of adult cattle, only one part of serum IgG (fast, anionic IgG) was precipitated by PEG. In older calves, all molecular forms of serum IgG had the capacity to form PEG precipitable immune complexes. Presented data, as well as the results of our previous work, can be used as parameters with reference to physicochemical and immunochemical characteristics of IgG immune complexes of preruminant calves under pathological conditions.
AB  - Telad od rođenja dolazi u kontakt sa velikim brojem antigena za čije je efikasno uklanjanje neophodno formiranje imunokompleksa. Sposobnost ovih, još uvek imunološki nezrelih, životinja da formiraju iminokomplekse, kao i sastav njihovih konstituenata, nisu detaljno ispitivani. U ovom radu smo imunokomplekse iz seruma teladi izolovali precipitacijom polietilen glikolom (PEGom), a osobine IgG, kao potencijalnih konstituenata imunokompleksa, analizirali metodama elektroforeze i imunoelektroforeze. U našem prethodnom radu dokazali smo da se nivo PEG precipitabilnih imunokompleksa menja sa uzrastom teladi. On raste od rođenja do 48. sata, opada desetog dana, ponovo raste u prvom mesecu, nakon čega ostaje nepromenjen do četvrtog meseca starosti. Elekroforetske i imunoelektroforetske analize su ukazale da se u serumu i PEG precipitatima seruma teladi do mesec dana starosti nalazi samo jedna molekulska frakcija IgG koja odgovara brzim, anjonskim IgG. Iako se u serumima teladi već u prvom mesecu starosti nalaze sve molekulske forme IgG koje se nalaze i u serumu odraslih krava, samo jedan njihov deo (brzi, anjonski γ globulini) ima sposobnost da formira imune komplekse. Kod starijih teladi, kao i kod odraslih krava, sve molekulske forme serumskih IgG mogu da formiraju imune komplekse. Ovi rezultati, zajedno sa rezultatima našeg predhodnog rada, se mogu koristiti za dobijanje referentnog sistema vrednosti nivoa i sastava imunokompleksa kod zdravih teladi, a koji bi se mogao koristiti za određivanje nivoa i sastava imunokompleksa kod teladi u patološkim uslovima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera
T1  - Elektroforetske i imunoelektroforetske karakteristike IgG kao konstituenata peg precipitabilnih imunokompleksa seruma kod teladi do četvrtog meseca starosti
EP  - 164
IS  - 2-3
SP  - 155
VL  - 60
DO  - 10.2298/AVB1003155F
ER  - 

@article{
author = "Fratrić, Natalija and Ilić, Vesna and Milošević-Jovčić, Nadežda and Stojić, V.",
year = "2010",
abstract = "Preruminant calves encounter numerous antigens, and formation of immune complexes is necessary for antigens elimination. The capability of immunologically immature calves to form immune complexes has not yet been studied in detail. For immune complexes studies, selective precipitation by PEG was performed, in combination with agarose gel electrophoresis and immunoelectrophoresis, with an aim to determine some properties of IgG, as constituents of immune complexes. In our previous work it was shown that the level of PEG precipitable immune complexes increased in the period from birth to 48-hours of life, decreased at day 10, increased in one month old animals, and after that, stayed unchanged until 4th month of age. Electrophoretic and immunoelectrophoretic analysis showed that until one month of age, calves' sera and PEG precipitates contained only one part of IgG molecules which corresponded to fast, anionic γ globulins. Although at the age of one month, preruminant calves' sera contained all molecular forms of IgG molecules present in the sera of adult cattle, only one part of serum IgG (fast, anionic IgG) was precipitated by PEG. In older calves, all molecular forms of serum IgG had the capacity to form PEG precipitable immune complexes. Presented data, as well as the results of our previous work, can be used as parameters with reference to physicochemical and immunochemical characteristics of IgG immune complexes of preruminant calves under pathological conditions., Telad od rođenja dolazi u kontakt sa velikim brojem antigena za čije je efikasno uklanjanje neophodno formiranje imunokompleksa. Sposobnost ovih, još uvek imunološki nezrelih, životinja da formiraju iminokomplekse, kao i sastav njihovih konstituenata, nisu detaljno ispitivani. U ovom radu smo imunokomplekse iz seruma teladi izolovali precipitacijom polietilen glikolom (PEGom), a osobine IgG, kao potencijalnih konstituenata imunokompleksa, analizirali metodama elektroforeze i imunoelektroforeze. U našem prethodnom radu dokazali smo da se nivo PEG precipitabilnih imunokompleksa menja sa uzrastom teladi. On raste od rođenja do 48. sata, opada desetog dana, ponovo raste u prvom mesecu, nakon čega ostaje nepromenjen do četvrtog meseca starosti. Elekroforetske i imunoelektroforetske analize su ukazale da se u serumu i PEG precipitatima seruma teladi do mesec dana starosti nalazi samo jedna molekulska frakcija IgG koja odgovara brzim, anjonskim IgG. Iako se u serumima teladi već u prvom mesecu starosti nalaze sve molekulske forme IgG koje se nalaze i u serumu odraslih krava, samo jedan njihov deo (brzi, anjonski γ globulini) ima sposobnost da formira imune komplekse. Kod starijih teladi, kao i kod odraslih krava, sve molekulske forme serumskih IgG mogu da formiraju imune komplekse. Ovi rezultati, zajedno sa rezultatima našeg predhodnog rada, se mogu koristiti za dobijanje referentnog sistema vrednosti nivoa i sastava imunokompleksa kod zdravih teladi, a koji bi se mogao koristiti za određivanje nivoa i sastava imunokompleksa kod teladi u patološkim uslovima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera, Elektroforetske i imunoelektroforetske karakteristike IgG kao konstituenata peg precipitabilnih imunokompleksa seruma kod teladi do četvrtog meseca starosti",
pages = "164-155",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003155F"
}

Fratrić, N., Ilić, V., Milošević-Jovčić, N.,& Stojić, V.. (2010). Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 155-164.
https://doi.org/10.2298/AVB1003155F

Fratrić N, Ilić V, Milošević-Jovčić N, Stojić V. Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera. in Acta Veterinaria-Beograd. 2010;60(2-3):155-164.
doi:10.2298/AVB1003155F .

Fratrić, Natalija, Ilić, Vesna, Milošević-Jovčić, Nadežda, Stojić, V., "Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):155-164,
https://doi.org/10.2298/AVB1003155F . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Milošević-Jovčić, Nadežda
AU  - Ilić, Vesna
AU  - Stojić, V.
PY  - 2006
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/153
AB  - In this work immune complexes (IC) in the sera of healthy calves from birth to four months of age, heifers and cows were investigated. Polyethylen glycol (PEG) precipitation assay was applied after previous standardization. Optical density (OD) of redissolved precipitates, formed after adding PEG to serum samples, was a measure of IC level. The upper-limit values for normal IC levels were established using isolated cattle IgG in different amounts and aggregated by heat as an IC model in vitro, in the same assay. In newborn calves before colostrum intake, OD of 0.047±0.024 was registered in redissolved PEG precipitates, far below the values obtained for native monomeric IgG. Therefore, these values can not be considered to refer to immune complexes. After colostrum intake, OD values for normal IC levels were higher, with significant interindividual differences: in calves at 6 24 and 48 hours after birth the mean OD values of solubilized PEG precipitates were 0.069±0.025, 0.148±0.079, and 0.136±0.062 respectively. In the sera of calves 10 days after birth mean OD value was slightly lower 0.089±0.053, whereas in the serum of 1, 2, 3 and 4 month old calves normal levels of IC rose to 0.165±0.067, 0.157±0.080, 0,231±0,124 and 0.261±0.092 respectively. OD values for IC levels for adult animals were found to be 0,456±0,150 for heifers, and 0,183±0,031 for cows. Together, the obtained data may be included in a reference values system for normal levels of IC at different ages, in relation to which the IC levels under pathological conditions could be estimated.
AB  - U radu je ispitivan nivo imunokompleksa (IC) u krvnom serumu teladi od rođenja do četiri meseca starosti, kao i kod junica i krava. Za analize je posle predhodne standardizacije korišćen polietilenglikol precipitacioni test (direktan PEG test). Meru nivoa IC predstavljale su vrednosti optičke gustine (OD) rastvorenog precipitata dobijenog nakon taloženja imunokompleksa iz seruma PEG-om. Gornja granica normalnih vrednosti za nivo IC utvrđena je prema vrednostima dobijenim za izolovane i toplotom agregirane kravlje IgG, u istom testu, gde su takvi IgG korišćeni kao modeli IC in vitro. U grupi novorođene teladi pre uzimanja kolostruma vrednosti OD su bile ekstremno niske (OD 0,047±0.024) i nisu mogle biti pripisane imunokompleksima. Vrednosti OD za normalan nivo IC u serumu teladi nakon uzimanja kolostruma su bile više: za telad 6, 24 i 48 sati nakon rođenja taj nivo je iznosio 0,069±0,025, 0,148±0,079 i 0,136±0,062 respektivno. U odnosu na ove vrednosti, nivo IC u serumu teladi 10 dana od rođenja je bio nešto niži i iznosio 0,089±0,053, da bi se, zatim, kontinuirano povećavao kod teladi uzrasta od 1 do 4 meseca i iznosio 0,165±0,067 za telad od 1-og meseca, 0,157±0,080 za telad od 2 meseca, 0,231±0,124 za telad od 3 meseca i 0,261±0,092 za telad od 4 meseca starosti. Nivo imunokompleksa u serumu odraslih goveda iznosio je kod junica 0,456±0,150 i krava 0,183±0,031. Dobijeni rezultati mogu biti uključeni u kontrolni sistem vrednosti za normalan nivo IC u raznim starosnim grupama goveda, koji se može koristiti pri određivanju nivoa IC PEG testom pod patološkim uslovima kod teladi junica i krava.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle
T1  - Imunokompleksi u krvnom serumu teladi u neonatalnom periodu i kod odraslih goveda
EP  - 110
IS  - 2-3
SP  - 103
VL  - 56
DO  - 10.2298/AVB0603103F
ER  - 

@article{
author = "Fratrić, Natalija and Milošević-Jovčić, Nadežda and Ilić, Vesna and Stojić, V.",
year = "2006",
abstract = "In this work immune complexes (IC) in the sera of healthy calves from birth to four months of age, heifers and cows were investigated. Polyethylen glycol (PEG) precipitation assay was applied after previous standardization. Optical density (OD) of redissolved precipitates, formed after adding PEG to serum samples, was a measure of IC level. The upper-limit values for normal IC levels were established using isolated cattle IgG in different amounts and aggregated by heat as an IC model in vitro, in the same assay. In newborn calves before colostrum intake, OD of 0.047±0.024 was registered in redissolved PEG precipitates, far below the values obtained for native monomeric IgG. Therefore, these values can not be considered to refer to immune complexes. After colostrum intake, OD values for normal IC levels were higher, with significant interindividual differences: in calves at 6 24 and 48 hours after birth the mean OD values of solubilized PEG precipitates were 0.069±0.025, 0.148±0.079, and 0.136±0.062 respectively. In the sera of calves 10 days after birth mean OD value was slightly lower 0.089±0.053, whereas in the serum of 1, 2, 3 and 4 month old calves normal levels of IC rose to 0.165±0.067, 0.157±0.080, 0,231±0,124 and 0.261±0.092 respectively. OD values for IC levels for adult animals were found to be 0,456±0,150 for heifers, and 0,183±0,031 for cows. Together, the obtained data may be included in a reference values system for normal levels of IC at different ages, in relation to which the IC levels under pathological conditions could be estimated., U radu je ispitivan nivo imunokompleksa (IC) u krvnom serumu teladi od rođenja do četiri meseca starosti, kao i kod junica i krava. Za analize je posle predhodne standardizacije korišćen polietilenglikol precipitacioni test (direktan PEG test). Meru nivoa IC predstavljale su vrednosti optičke gustine (OD) rastvorenog precipitata dobijenog nakon taloženja imunokompleksa iz seruma PEG-om. Gornja granica normalnih vrednosti za nivo IC utvrđena je prema vrednostima dobijenim za izolovane i toplotom agregirane kravlje IgG, u istom testu, gde su takvi IgG korišćeni kao modeli IC in vitro. U grupi novorođene teladi pre uzimanja kolostruma vrednosti OD su bile ekstremno niske (OD 0,047±0.024) i nisu mogle biti pripisane imunokompleksima. Vrednosti OD za normalan nivo IC u serumu teladi nakon uzimanja kolostruma su bile više: za telad 6, 24 i 48 sati nakon rođenja taj nivo je iznosio 0,069±0,025, 0,148±0,079 i 0,136±0,062 respektivno. U odnosu na ove vrednosti, nivo IC u serumu teladi 10 dana od rođenja je bio nešto niži i iznosio 0,089±0,053, da bi se, zatim, kontinuirano povećavao kod teladi uzrasta od 1 do 4 meseca i iznosio 0,165±0,067 za telad od 1-og meseca, 0,157±0,080 za telad od 2 meseca, 0,231±0,124 za telad od 3 meseca i 0,261±0,092 za telad od 4 meseca starosti. Nivo imunokompleksa u serumu odraslih goveda iznosio je kod junica 0,456±0,150 i krava 0,183±0,031. Dobijeni rezultati mogu biti uključeni u kontrolni sistem vrednosti za normalan nivo IC u raznim starosnim grupama goveda, koji se može koristiti pri određivanju nivoa IC PEG testom pod patološkim uslovima kod teladi junica i krava.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle, Imunokompleksi u krvnom serumu teladi u neonatalnom periodu i kod odraslih goveda",
pages = "110-103",
number = "2-3",
volume = "56",
doi = "10.2298/AVB0603103F"
}

Fratrić, N., Milošević-Jovčić, N., Ilić, V.,& Stojić, V.. (2006). The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(2-3), 103-110.
https://doi.org/10.2298/AVB0603103F

Fratrić N, Milošević-Jovčić N, Ilić V, Stojić V. The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle. in Acta Veterinaria-Beograd. 2006;56(2-3):103-110.
doi:10.2298/AVB0603103F .

Fratrić, Natalija, Milošević-Jovčić, Nadežda, Ilić, Vesna, Stojić, V., "The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle" in Acta Veterinaria-Beograd, 56, no. 2-3 (2006):103-110,
https://doi.org/10.2298/AVB0603103F . .