TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Milanović, Slađan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Miletić, Maja
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1474
AB  - The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients
IS  - 6
SP  - 3138
VL  - 25
DO  - 10.3390/ijms25063138
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Milanović, Slađan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Miletić, Maja and Bugarski, Branko and Ilić, Vesna",
year = "2024",
abstract = "The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients",
number = "6",
pages = "3138",
volume = "25",
doi = "10.3390/ijms25063138"
}

Đukić, T., Drvenica, I., Kovačić, M., Milanović, S., Majerič, D., Šefik-Bukilica, M., Miletić, M., Bugarski, B.,& Ilić, V.. (2024). Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 25(6), 3138.
https://doi.org/10.3390/ijms25063138

Đukić T, Drvenica I, Kovačić M, Milanović S, Majerič D, Šefik-Bukilica M, Miletić M, Bugarski B, Ilić V. Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences. 2024;25(6):3138.
doi:10.3390/ijms25063138 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Milanović, Slađan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Miletić, Maja, Bugarski, Branko, Ilić, Vesna, "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients" in International Journal of Molecular Sciences, 25, no. 6 (2024):3138,
https://doi.org/10.3390/ijms25063138 . .

TY  - CONF
AU  - Ristić, Biljana
AU  - Trpkov, Đorđe
AU  - Drvenica, Ivana
AU  - Dojčilović, Radovan
AU  - Đukić, Tamara
AU  - Tošić, Dragana
AU  - Pajović, Jelena
AU  - Božanić, Dušan K.
AU  - Trivanović, Drenka
AU  - Matić, Tamara
AU  - Sredojević, Dušan
AU  - Ilić, Vesna
AU  - Đoković, Vladimir
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1471
AB  - Nitrogen-doped carbon quantum dots (N-CQDs) are promising next
generation nanomaterials for potential biomedical applications such as bioimaging, biosensing,
and drug/gene delivery. However, N-CQDs biocompatibility has not been extensively
investigated. Here, we report physico-chemical characteristics of newly synthetized N-CQDs
and their effects on red blood cells (RBC), by analyzing their hemolytic activity, impact on
RBC rheology/morphology, and oxidative stress induction. N-CQDs were prepared by
hydrothermal method using citric acid and urea as precursors. Structural analyses of as prepared
N-GQDs, observed by HRTEM/EDS, showed that the lateral dimensions of the particles are in
the 10 to 20 nm range, as well as that the carbon, oxygen, and nitrogen are present in the
nanosystem. Based on AFM measurements, the average height of N-CQDs was 3.9±0.08 Å.
Photoluminescence emission (PLE) spectrum demonstrated that N-CQDs exhibit stable and
strong fluorescence in green (520 nm) region, upon 410 nm excitation. FTIR spectroscopy
indicated vibrational bands, characteristic for carbon structures and primary amines (Ndoping).
N-CQDs were negatively charged with an average Zeta potential of -15.3
mV as confirmed by DLS. To investigate hemocompatibility of N-CQDs, the RBC, the most
abundant cells in blood, were treated with different concentration of N-CQDs (10-400 ug/ml)
for 2h. Obtained results showed that there was no hemolytic activity. Moreover, ektacytometry
analysis demonstrated that N-CQDs did not affect deformability of RBC. Fluorescent
microscopy analyses revealed that treatment with N-CQDs did not induce significant
morphological aberrant forms of RBC which was also confirmed by SEM analyses. Flow
cytometry confirmed only slight RBC morphological changes based on FSC/SSC analysis.
Furthermore, using ROS sensitive dye flow cytometry analyses suggested that N-CQDs did not
induce oxidative stress in RBC. Taken together, our findings highlighted that exposure of RBC
to N-CQDs only led to the attachment of N-CQDs on RBC membranes, but there is no other
evidence of their nanotoxicity. These findings suggested that N-CQDs synthetized from ecofriendly
precursors are potentially biocompatible and safe for biomedical application.
PB  - Belgrade : Institute of Physics
C3  - Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
T1  - Hemocompatibility evaluation of N-doped carbon quantum dots
EP  - 47
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1471
ER  - 

@conference{
author = "Ristić, Biljana and Trpkov, Đorđe and Drvenica, Ivana and Dojčilović, Radovan and Đukić, Tamara and Tošić, Dragana and Pajović, Jelena and Božanić, Dušan K. and Trivanović, Drenka and Matić, Tamara and Sredojević, Dušan and Ilić, Vesna and Đoković, Vladimir",
year = "2024",
abstract = "Nitrogen-doped carbon quantum dots (N-CQDs) are promising next
generation nanomaterials for potential biomedical applications such as bioimaging, biosensing,
and drug/gene delivery. However, N-CQDs biocompatibility has not been extensively
investigated. Here, we report physico-chemical characteristics of newly synthetized N-CQDs
and their effects on red blood cells (RBC), by analyzing their hemolytic activity, impact on
RBC rheology/morphology, and oxidative stress induction. N-CQDs were prepared by
hydrothermal method using citric acid and urea as precursors. Structural analyses of as prepared
N-GQDs, observed by HRTEM/EDS, showed that the lateral dimensions of the particles are in
the 10 to 20 nm range, as well as that the carbon, oxygen, and nitrogen are present in the
nanosystem. Based on AFM measurements, the average height of N-CQDs was 3.9±0.08 Å.
Photoluminescence emission (PLE) spectrum demonstrated that N-CQDs exhibit stable and
strong fluorescence in green (520 nm) region, upon 410 nm excitation. FTIR spectroscopy
indicated vibrational bands, characteristic for carbon structures and primary amines (Ndoping).
N-CQDs were negatively charged with an average Zeta potential of -15.3
mV as confirmed by DLS. To investigate hemocompatibility of N-CQDs, the RBC, the most
abundant cells in blood, were treated with different concentration of N-CQDs (10-400 ug/ml)
for 2h. Obtained results showed that there was no hemolytic activity. Moreover, ektacytometry
analysis demonstrated that N-CQDs did not affect deformability of RBC. Fluorescent
microscopy analyses revealed that treatment with N-CQDs did not induce significant
morphological aberrant forms of RBC which was also confirmed by SEM analyses. Flow
cytometry confirmed only slight RBC morphological changes based on FSC/SSC analysis.
Furthermore, using ROS sensitive dye flow cytometry analyses suggested that N-CQDs did not
induce oxidative stress in RBC. Taken together, our findings highlighted that exposure of RBC
to N-CQDs only led to the attachment of N-CQDs on RBC membranes, but there is no other
evidence of their nanotoxicity. These findings suggested that N-CQDs synthetized from ecofriendly
precursors are potentially biocompatible and safe for biomedical application.",
publisher = "Belgrade : Institute of Physics",
journal = "Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024",
title = "Hemocompatibility evaluation of N-doped carbon quantum dots",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1471"
}

Ristić, B., Trpkov, Đ., Drvenica, I., Dojčilović, R., Đukić, T., Tošić, D., Pajović, J., Božanić, D. K., Trivanović, D., Matić, T., Sredojević, D., Ilić, V.,& Đoković, V.. (2024). Hemocompatibility evaluation of N-doped carbon quantum dots. in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
Belgrade : Institute of Physics., 47-47.
https://hdl.handle.net/21.15107/rcub_rimi_1471

Ristić B, Trpkov Đ, Drvenica I, Dojčilović R, Đukić T, Tošić D, Pajović J, Božanić DK, Trivanović D, Matić T, Sredojević D, Ilić V, Đoković V. Hemocompatibility evaluation of N-doped carbon quantum dots. in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024. 2024;:47-47.
https://hdl.handle.net/21.15107/rcub_rimi_1471 .

Ristić, Biljana, Trpkov, Đorđe, Drvenica, Ivana, Dojčilović, Radovan, Đukić, Tamara, Tošić, Dragana, Pajović, Jelena, Božanić, Dušan K., Trivanović, Drenka, Matić, Tamara, Sredojević, Dušan, Ilić, Vesna, Đoković, Vladimir, "Hemocompatibility evaluation of N-doped carbon quantum dots" in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 (2024):47-47,
https://hdl.handle.net/21.15107/rcub_rimi_1471 .

TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Minić, Rajna
AU  - Vučetić, Dušan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Savić, Olivera
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1314
AB  - The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.
PB  - Elsevier
T2  - Analytical Biochemistry
T2  - Analytical BiochemistryAnalytical Biochemistry
T1  - Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients
SP  - 115194
VL  - 674
DO  - 10.1016/j.ab.2023.115194
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Minić, Rajna and Vučetić, Dušan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Savić, Olivera and Bugarski, Branko and Ilić, Vesna",
year = "2023",
abstract = "The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.",
publisher = "Elsevier",
journal = "Analytical Biochemistry, Analytical BiochemistryAnalytical Biochemistry",
title = "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients",
pages = "115194",
volume = "674",
doi = "10.1016/j.ab.2023.115194"
}

Đukić, T., Drvenica, I., Kovačić, M., Minić, R., Vučetić, D., Majerič, D., Šefik-Bukilica, M., Savić, O., Bugarski, B.,& Ilić, V.. (2023). Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry
Elsevier., 674, 115194.
https://doi.org/10.1016/j.ab.2023.115194

Đukić T, Drvenica I, Kovačić M, Minić R, Vučetić D, Majerič D, Šefik-Bukilica M, Savić O, Bugarski B, Ilić V. Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry. 2023;674:115194.
doi:10.1016/j.ab.2023.115194 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Minić, Rajna, Vučetić, Dušan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Savić, Olivera, Bugarski, Branko, Ilić, Vesna, "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients" in Analytical Biochemistry, 674 (2023):115194,
https://doi.org/10.1016/j.ab.2023.115194 . .

TY  - CONF
AU  - Drvenica, Ivana
AU  - Trivanović, Drenka
AU  - Radmilović, Mihajlo
AU  - Vučetić, Dušan
AU  - Krmpot, Aleksandar
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1405
AB  - Ektacytometry, a diffraction-based method, measures the deformability of entire erythrocyte populations and does not provide information on the altered deformability of individual erythrocytes or affected subpopulation induced by constant oxidative stress, physical stress, metabolic depletion, and loss of ion gradients during their 120 days life span. We introduced an approach based on ektacytometry coupled to flow cytometry analysis to monitor the deformability and heterogeneity of subpopulations of erythrocytes. The effects of in vitro changes of osmotic gradient (from 155 mM to 93 mM phosphate buffer) and treatment by oxidative agent (0.5 mM and 0.75 mM terc-bytil hydroperoxide (TBPH)) on human erythrocyte isolated from healthy male donors, were tested using RheoScan D 300 (RheoMeditech. Inc., Korea) and BD FACSCalibur flow cytometer (Becton Dickinson, USA).
A decrease in erythrocyte deformability by changes in osmolality or treatment with TBPH 
per se was demonstrated by ektacytometry. Nevertheless, this method could not analyze the 
erythrocytes that underwent both treatments due to their lysis by the shear stress in the 
device. The samples of an equal population of normal erythrocytes and erythrocytes 
rigidified by 0.5 mM TBPH (slightly rigid) showed elongation indices in the physiological 
range, i.e., the effect of the treatments was annulled. The same result was obtained by flow 
cytometry. On the other hand, an altered population of oxidized cells by 0.5 mM TBPH was 
detected in hypoosmotic 93 mM buffer based on their forward scatter (FSC) (Figure 1) and 
side scatter (SSC) parameters. The subtle changes in the erythrocyte’s subpopulation 
mechanobiology are essential to monitoring even in healthy people exposed to physical 
or/and environmental stress and stored erythrocytes, but some additional techniques are 
needed for the established optical-based approaches.
PB  - Belgrade: Institute of Physics
C3  - 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts
T1  - Optical methodologies in the analysis of erythrocyte deformability and heterogeneity
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1405
ER  - 

@conference{
author = "Drvenica, Ivana and Trivanović, Drenka and Radmilović, Mihajlo and Vučetić, Dušan and Krmpot, Aleksandar and Ilić, Vesna",
year = "2023",
abstract = "Ektacytometry, a diffraction-based method, measures the deformability of entire erythrocyte populations and does not provide information on the altered deformability of individual erythrocytes or affected subpopulation induced by constant oxidative stress, physical stress, metabolic depletion, and loss of ion gradients during their 120 days life span. We introduced an approach based on ektacytometry coupled to flow cytometry analysis to monitor the deformability and heterogeneity of subpopulations of erythrocytes. The effects of in vitro changes of osmotic gradient (from 155 mM to 93 mM phosphate buffer) and treatment by oxidative agent (0.5 mM and 0.75 mM terc-bytil hydroperoxide (TBPH)) on human erythrocyte isolated from healthy male donors, were tested using RheoScan D 300 (RheoMeditech. Inc., Korea) and BD FACSCalibur flow cytometer (Becton Dickinson, USA).
A decrease in erythrocyte deformability by changes in osmolality or treatment with TBPH 
per se was demonstrated by ektacytometry. Nevertheless, this method could not analyze the 
erythrocytes that underwent both treatments due to their lysis by the shear stress in the 
device. The samples of an equal population of normal erythrocytes and erythrocytes 
rigidified by 0.5 mM TBPH (slightly rigid) showed elongation indices in the physiological 
range, i.e., the effect of the treatments was annulled. The same result was obtained by flow 
cytometry. On the other hand, an altered population of oxidized cells by 0.5 mM TBPH was 
detected in hypoosmotic 93 mM buffer based on their forward scatter (FSC) (Figure 1) and 
side scatter (SSC) parameters. The subtle changes in the erythrocyte’s subpopulation 
mechanobiology are essential to monitoring even in healthy people exposed to physical 
or/and environmental stress and stored erythrocytes, but some additional techniques are 
needed for the established optical-based approaches.",
publisher = "Belgrade: Institute of Physics",
journal = "16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts",
title = "Optical methodologies in the analysis of erythrocyte deformability and heterogeneity",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1405"
}

Drvenica, I., Trivanović, D., Radmilović, M., Vučetić, D., Krmpot, A.,& Ilić, V.. (2023). Optical methodologies in the analysis of erythrocyte deformability and heterogeneity. in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts
Belgrade: Institute of Physics., 27-27.
https://hdl.handle.net/21.15107/rcub_rimi_1405

Drvenica I, Trivanović D, Radmilović M, Vučetić D, Krmpot A, Ilić V. Optical methodologies in the analysis of erythrocyte deformability and heterogeneity. in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts. 2023;:27-27.
https://hdl.handle.net/21.15107/rcub_rimi_1405 .

Drvenica, Ivana, Trivanović, Drenka, Radmilović, Mihajlo, Vučetić, Dušan, Krmpot, Aleksandar, Ilić, Vesna, "Optical methodologies in the analysis of erythrocyte deformability and heterogeneity" in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts (2023):27-27,
https://hdl.handle.net/21.15107/rcub_rimi_1405 .

TY  - JOUR
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Kovačić, Marijana
AU  - Vignjević-Petrinović, Sanja
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1288
AB  - Myeloproliferative neoplasms (MPNs) are hematologic malignancies characterized by gene mutations that promote myeloproliferation and resistance to apoptosis via constitutively active signaling pathways, with Janus kinase 2-signal transducers and the activators of transcription (JAK-STAT) axis as a core part. Chronic inflammation has been described as a pivot for the development and advancement of MPNs from early stage cancer to pronounced bone marrow fibrosis, but there are still unresolved questions regarding this issue. The MPN neutrophils are characterized by upregulation of JAK target genes, they are in a state of activation and with deregulated apoptotic machinery. Deregulated neutrophil apoptotic cell death supports inflammation and steers them towards secondary necrosis or neutrophil extracellular trap (NET) formation, a trigger of inflammation both ways. NETs in proinflammatory bone marrow microenvironment induce hematopoietic precursor proliferation, which has an impact on hematopoietic disorders. In MPNs, neutrophils are primed for NET formation, and even though it seems obvious for NETs to intervene in the disease progression by supporting inflammation, no reliable data are available. We discuss in this review the potential pathophysiological relevance of NET formation in MPNs, with the intention of contributing to a better understanding of how neutrophils and neutrophil clonality can orchestrate the evolution of a pathological microenvironment in MPNs.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms
IS  - 5
SP  - 4497
VL  - 24
DO  - 10.3390/ijms24054497
ER  - 

@article{
author = "Marković, Dragana and Maslovarić, Irina and Kovačić, Marijana and Vignjević-Petrinović, Sanja and Ilić, Vesna",
year = "2023",
abstract = "Myeloproliferative neoplasms (MPNs) are hematologic malignancies characterized by gene mutations that promote myeloproliferation and resistance to apoptosis via constitutively active signaling pathways, with Janus kinase 2-signal transducers and the activators of transcription (JAK-STAT) axis as a core part. Chronic inflammation has been described as a pivot for the development and advancement of MPNs from early stage cancer to pronounced bone marrow fibrosis, but there are still unresolved questions regarding this issue. The MPN neutrophils are characterized by upregulation of JAK target genes, they are in a state of activation and with deregulated apoptotic machinery. Deregulated neutrophil apoptotic cell death supports inflammation and steers them towards secondary necrosis or neutrophil extracellular trap (NET) formation, a trigger of inflammation both ways. NETs in proinflammatory bone marrow microenvironment induce hematopoietic precursor proliferation, which has an impact on hematopoietic disorders. In MPNs, neutrophils are primed for NET formation, and even though it seems obvious for NETs to intervene in the disease progression by supporting inflammation, no reliable data are available. We discuss in this review the potential pathophysiological relevance of NET formation in MPNs, with the intention of contributing to a better understanding of how neutrophils and neutrophil clonality can orchestrate the evolution of a pathological microenvironment in MPNs.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms",
number = "5",
pages = "4497",
volume = "24",
doi = "10.3390/ijms24054497"
}

Marković, D., Maslovarić, I., Kovačić, M., Vignjević-Petrinović, S.,& Ilić, V.. (2023). Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 24(5), 4497.
https://doi.org/10.3390/ijms24054497

Marković D, Maslovarić I, Kovačić M, Vignjević-Petrinović S, Ilić V. Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms. in International Journal of Molecular Sciences. 2023;24(5):4497.
doi:10.3390/ijms24054497 .

Marković, Dragana, Maslovarić, Irina, Kovačić, Marijana, Vignjević-Petrinović, Sanja, Ilić, Vesna, "Putative Role of Neutrophil Extracellular Trap Formation in Chronic Myeloproliferative Neoplasms" in International Journal of Molecular Sciences, 24, no. 5 (2023):4497,
https://doi.org/10.3390/ijms24054497 . .

TY  - CONF
AU  - Minić, Rajna
AU  - Đorđević, Brižita
AU  - Michaličkova, Danica
AU  - Živković, Irena
AU  - Živančević Simonović, Snežana
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1407
AB  - U ovom izlaganju biće predstavljeni naši dosadašnji rezultati na temu antimikrobnih antitela kod profesionalnih sportista, odraslih osoba koje se ne bave profesionalno sportom; promene nivoa antibakterijskih antitela pri starenju; poređenje specifičnosti serumskih i salivarnih IgA antitela kod mladih odraslih osoba, kao i nivoi ukupnih i antibakterijskih antitela u Covid-19 virusnoj infekciji i u sepsi. Nivoi i titri antimikrobnih antitela različitih klasa i potklasa određivani su ELISA testom iz uzoraka seruma, plazme ili salive. Ispitivano je vezivanje za izolovane antigene mikroorganizama (lipopolisaharid, peptidoglikan i zimozan) i za cele mikroorganizme, različitih vrsta i sojeva. Vezivanje prečišćenog salivarnog IgA za mikroorganizme praćeno je i protočnom citometrijom. Od rezultata izdvajamo da izlaganje fizičkom naporu visokog intenziteta dovodi do promena na nivou antibakterijskih antitela, i to u pravcu sveobuhvatnijeg prepoznavanja lipopolisaharida1. Suplementacija profesionalnih sportista određenim probioticima dovodi do održanja nivoa ukupnih salivarnih i serumskih IgA antitela, kao i IgG antitela specifičnih prema Enterococcus faecalis, koja su u ovoj populaciji pokazala sezonsku varijaciju. Pri starenju dolazi do smanjenja nivoa antipneumokoknih antitela i to zavisno od pola, pa su stariji mukarci naročito pogođeni ovim smanjenjem3. Salivarna IgA antitela razlikuju se po specifičnosti od serumskih IgA antitela i pružaju nespecifičnu zaštitu. Poređenje pacijenata sa sepsom, hospitalizovanih Covid-19 pacijenta i starosnih kontrola pokazalo je brojne razlike u nivoima kako ukupnih, tako i antibakterijskih antitela. Nivoi antibakterijskih antitela kod pacijentata koji nisu preživeli bili su niži nego kod onih koji su preživeli, što potvrđuje značaj ovih anititela za preživljavanje sepse.
PB  - Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije
C3  - Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
T1  - Antimikrobna antitela u fiziološkim i patološkim stanjima
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1407
ER  - 

@conference{
author = "Minić, Rajna and Đorđević, Brižita and Michaličkova, Danica and Živković, Irena and Živančević Simonović, Snežana and Ilić, Vesna",
year = "2023",
abstract = "U ovom izlaganju biće predstavljeni naši dosadašnji rezultati na temu antimikrobnih antitela kod profesionalnih sportista, odraslih osoba koje se ne bave profesionalno sportom; promene nivoa antibakterijskih antitela pri starenju; poređenje specifičnosti serumskih i salivarnih IgA antitela kod mladih odraslih osoba, kao i nivoi ukupnih i antibakterijskih antitela u Covid-19 virusnoj infekciji i u sepsi. Nivoi i titri antimikrobnih antitela različitih klasa i potklasa određivani su ELISA testom iz uzoraka seruma, plazme ili salive. Ispitivano je vezivanje za izolovane antigene mikroorganizama (lipopolisaharid, peptidoglikan i zimozan) i za cele mikroorganizme, različitih vrsta i sojeva. Vezivanje prečišćenog salivarnog IgA za mikroorganizme praćeno je i protočnom citometrijom. Od rezultata izdvajamo da izlaganje fizičkom naporu visokog intenziteta dovodi do promena na nivou antibakterijskih antitela, i to u pravcu sveobuhvatnijeg prepoznavanja lipopolisaharida1. Suplementacija profesionalnih sportista određenim probioticima dovodi do održanja nivoa ukupnih salivarnih i serumskih IgA antitela, kao i IgG antitela specifičnih prema Enterococcus faecalis, koja su u ovoj populaciji pokazala sezonsku varijaciju. Pri starenju dolazi do smanjenja nivoa antipneumokoknih antitela i to zavisno od pola, pa su stariji mukarci naročito pogođeni ovim smanjenjem3. Salivarna IgA antitela razlikuju se po specifičnosti od serumskih IgA antitela i pružaju nespecifičnu zaštitu. Poređenje pacijenata sa sepsom, hospitalizovanih Covid-19 pacijenta i starosnih kontrola pokazalo je brojne razlike u nivoima kako ukupnih, tako i antibakterijskih antitela. Nivoi antibakterijskih antitela kod pacijentata koji nisu preživeli bili su niži nego kod onih koji su preživeli, što potvrđuje značaj ovih anititela za preživljavanje sepse.",
publisher = "Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije",
journal = "Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka",
title = "Antimikrobna antitela u fiziološkim i patološkim stanjima",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1407"
}

Minić, R., Đorđević, B., Michaličkova, D., Živković, I., Živančević Simonović, S.,& Ilić, V.. (2023). Antimikrobna antitela u fiziološkim i patološkim stanjima. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije..
https://hdl.handle.net/21.15107/rcub_rimi_1407

Minić R, Đorđević B, Michaličkova D, Živković I, Živančević Simonović S, Ilić V. Antimikrobna antitela u fiziološkim i patološkim stanjima. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka. 2023;.
https://hdl.handle.net/21.15107/rcub_rimi_1407 .

Minić, Rajna, Đorđević, Brižita, Michaličkova, Danica, Živković, Irena, Živančević Simonović, Snežana, Ilić, Vesna, "Antimikrobna antitela u fiziološkim i patološkim stanjima" in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka (2023),
https://hdl.handle.net/21.15107/rcub_rimi_1407 .

TY  - JOUR
AU  - Radmilović, Mihajlo D.
AU  - Drvenica, Ivana
AU  - Rabasović, Mihailo D.
AU  - Ilić, Vesna
AU  - Pavlović, Danica
AU  - Oasa, Sho
AU  - Vukojević, Vladana
AU  - Perić, Mina
AU  - Nikolić, Stanko N.
AU  - Krmpot, Aleksandar J.
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1324
AB  - Hemoglobin (Hb), a life-sustaining and highly abundant erythrocyte protein, is not readily fluorescent. A few studies have already reported Two-Photon Excited Fluorescence (TPEF) of Hb, however, the mechanisms through which Hb becomes fluorescent upon interaction with ultrashort laser pulses are not completely understood. Here, we characterized photophysically this interaction on Hb thin film and erythrocytes using fluorescence spectroscopy upon single-photon/two-photon absorption, and UV-VIS single-photon absorption spectroscopy. A gradual increase of the fluorescence intensity, ending up with saturation, is observed upon prolonged exposure of Hb thin layer and erythrocytes to ultrashort laser pulses at 730 nm. When compared to protoporphyrin IX (PpIX) and oxidized Hb by H2O2, TPEF spectra from a thin Hb film and erythrocytes showed good mutual agreement, broad peaking at 550 nm, supporting hemoglobin undergoes degradation and that same fluorescent specie(s) originating from the heme moiety are generated. The uniform square shaped patterns of the fluorescent photoproduct exhibited the same level of the fluorescence intensity even after 12 weeks from the formation, indicating high photoproduct stability. We finally demonstrated the full potential of the formed Hb photoproduct with TPEF scanning microscopy towards spatiotemporally controlled micropatterning in HTF and single human erythrocyte labelling and tracking in the whole blood.
T2  - International Journal of Biological Macromolecules
T2  - International Journal of Biological Macromolecules
T1  - Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications
SP  - 125312
VL  - 244
DO  - 10.1016/j.ijbiomac.2023.125312
ER  - 

@article{
author = "Radmilović, Mihajlo D. and Drvenica, Ivana and Rabasović, Mihailo D. and Ilić, Vesna and Pavlović, Danica and Oasa, Sho and Vukojević, Vladana and Perić, Mina and Nikolić, Stanko N. and Krmpot, Aleksandar J.",
year = "2023",
abstract = "Hemoglobin (Hb), a life-sustaining and highly abundant erythrocyte protein, is not readily fluorescent. A few studies have already reported Two-Photon Excited Fluorescence (TPEF) of Hb, however, the mechanisms through which Hb becomes fluorescent upon interaction with ultrashort laser pulses are not completely understood. Here, we characterized photophysically this interaction on Hb thin film and erythrocytes using fluorescence spectroscopy upon single-photon/two-photon absorption, and UV-VIS single-photon absorption spectroscopy. A gradual increase of the fluorescence intensity, ending up with saturation, is observed upon prolonged exposure of Hb thin layer and erythrocytes to ultrashort laser pulses at 730 nm. When compared to protoporphyrin IX (PpIX) and oxidized Hb by H2O2, TPEF spectra from a thin Hb film and erythrocytes showed good mutual agreement, broad peaking at 550 nm, supporting hemoglobin undergoes degradation and that same fluorescent specie(s) originating from the heme moiety are generated. The uniform square shaped patterns of the fluorescent photoproduct exhibited the same level of the fluorescence intensity even after 12 weeks from the formation, indicating high photoproduct stability. We finally demonstrated the full potential of the formed Hb photoproduct with TPEF scanning microscopy towards spatiotemporally controlled micropatterning in HTF and single human erythrocyte labelling and tracking in the whole blood.",
journal = "International Journal of Biological Macromolecules, International Journal of Biological Macromolecules",
title = "Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications",
pages = "125312",
volume = "244",
doi = "10.1016/j.ijbiomac.2023.125312"
}

Radmilović, M. D., Drvenica, I., Rabasović, M. D., Ilić, V., Pavlović, D., Oasa, S., Vukojević, V., Perić, M., Nikolić, S. N.,& Krmpot, A. J.. (2023). Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications. in International Journal of Biological Macromolecules, 244, 125312.
https://doi.org/10.1016/j.ijbiomac.2023.125312

Radmilović MD, Drvenica I, Rabasović MD, Ilić V, Pavlović D, Oasa S, Vukojević V, Perić M, Nikolić SN, Krmpot AJ. Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications. in International Journal of Biological Macromolecules. 2023;244:125312.
doi:10.1016/j.ijbiomac.2023.125312 .

Radmilović, Mihajlo D., Drvenica, Ivana, Rabasović, Mihailo D., Ilić, Vesna, Pavlović, Danica, Oasa, Sho, Vukojević, Vladana, Perić, Mina, Nikolić, Stanko N., Krmpot, Aleksandar J., "Interactions of ultrashort laser pulses with hemoglobin: Photophysical aspects and potential applications" in International Journal of Biological Macromolecules, 244 (2023):125312,
https://doi.org/10.1016/j.ijbiomac.2023.125312 . .

TY  - JOUR
AU  - Knežević, Sanja
AU  - Kosanović, Dejana
AU  - Dragačević, Luka
AU  - Živković, Irena
AU  - Ilić, Vesna
AU  - Hajduković, Ljiljana
AU  - Savić, Olivera
AU  - Minić, Rajna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1255
AB  - S. pneumoniae is an important human pathogen which has a polysaccharide capsule with virulent properties. This work aims to estimate the titres of S. pneumoniae specific IgG and IgA isotypes, with respect to age and sex. An in-house whole bacterial cell ELISA was used for the determination of relative levels and endpoint titres of IgG subclasses and IgA1 subclass specific for S. pneumoniae serogroup 1, and to quantify specific IgG1 and IgG2 levels. Significantly lower anti-pneumococcus IgG1 titres were found in older individuals, which was more pronounced in men. Lower IgG2 titres were detected in men over 50 years of age, in comparison to women under 50 years of age. The levels of IgG3 and IgG4 did not differ between different sex and age groups. Lower IgA1 levels were detected in male individuals in both age groups in comparison to females under 50 years of age. The levels of IgG1 showed a moderate correlation with IgG4 in younger individuals of both sexes (r = 0.61 in men and 0.63 in women) which was not noted in the older age group. We highlight the deficiency in humoral immunity in older people, especially male and suggest immunization of this population with pneumococcal vaccines.
PB  - Elsevier
T2  - Comparative Immunology, Microbiology and Infectious Diseases
T1  - Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1
SP  - 101834
VL  - 87
DO  - 10.1016/j.cimid.2022.101834
ER  - 

@article{
author = "Knežević, Sanja and Kosanović, Dejana and Dragačević, Luka and Živković, Irena and Ilić, Vesna and Hajduković, Ljiljana and Savić, Olivera and Minić, Rajna",
year = "2022",
abstract = "S. pneumoniae is an important human pathogen which has a polysaccharide capsule with virulent properties. This work aims to estimate the titres of S. pneumoniae specific IgG and IgA isotypes, with respect to age and sex. An in-house whole bacterial cell ELISA was used for the determination of relative levels and endpoint titres of IgG subclasses and IgA1 subclass specific for S. pneumoniae serogroup 1, and to quantify specific IgG1 and IgG2 levels. Significantly lower anti-pneumococcus IgG1 titres were found in older individuals, which was more pronounced in men. Lower IgG2 titres were detected in men over 50 years of age, in comparison to women under 50 years of age. The levels of IgG3 and IgG4 did not differ between different sex and age groups. Lower IgA1 levels were detected in male individuals in both age groups in comparison to females under 50 years of age. The levels of IgG1 showed a moderate correlation with IgG4 in younger individuals of both sexes (r = 0.61 in men and 0.63 in women) which was not noted in the older age group. We highlight the deficiency in humoral immunity in older people, especially male and suggest immunization of this population with pneumococcal vaccines.",
publisher = "Elsevier",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
title = "Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1",
pages = "101834",
volume = "87",
doi = "10.1016/j.cimid.2022.101834"
}

Knežević, S., Kosanović, D., Dragačević, L., Živković, I., Ilić, V., Hajduković, L., Savić, O.,& Minić, R.. (2022). Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1. in Comparative Immunology, Microbiology and Infectious Diseases
Elsevier., 87, 101834.
https://doi.org/10.1016/j.cimid.2022.101834

Knežević S, Kosanović D, Dragačević L, Živković I, Ilić V, Hajduković L, Savić O, Minić R. Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1. in Comparative Immunology, Microbiology and Infectious Diseases. 2022;87:101834.
doi:10.1016/j.cimid.2022.101834 .

Knežević, Sanja, Kosanović, Dejana, Dragačević, Luka, Živković, Irena, Ilić, Vesna, Hajduković, Ljiljana, Savić, Olivera, Minić, Rajna, "Age and gender associated changes in immunoglobulin subclass levels specific to S. pneumoniae, serotype 1" in Comparative Immunology, Microbiology and Infectious Diseases, 87 (2022):101834,
https://doi.org/10.1016/j.cimid.2022.101834 . .

TY  - JOUR
AU  - Nikodijević, Slavomir
AU  - Blagojević, Veljko
AU  - Ćuruvija, Ivana
AU  - Kosanović, Dejana
AU  - Đukić, Tamara
AU  - Đorđević, Brižita
AU  - Ilić, Vesna
AU  - Minić, Rajna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1336
AB  - Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.
PB  - Wiley-Blackwell
T2  - Scandinavian Journal of Immunology
T1  - Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans
IS  - 6
SP  - e13223
VL  - 96
DO  - 10.1111/sji.13223
ER  - 

@article{
author = "Nikodijević, Slavomir and Blagojević, Veljko and Ćuruvija, Ivana and Kosanović, Dejana and Đukić, Tamara and Đorđević, Brižita and Ilić, Vesna and Minić, Rajna",
year = "2022",
abstract = "Increased interest in microbiota calls for the thorough analysis of antibody reactivity to different microorganisms. As salivary IgA represents the first line of defence against microorganisms contacting mucosal surfaces, we explored the binding and specificity of salivary IgA by testing the binding of purified, FITC-labelled salivary IgA to different microorganisms in flow cytometry and conclude that this kind of analysis enables the differentiation of species/strains with high IgA binding capacity, which should be corroborated on a larger sample size. Further we compare, with in-house ELISA, the binding of polyclonal salivary IgA with the binding of polyclonal serum IgA from the same individuals to whole microbial cells and to purified microbial components. High correlations were obtained in total salivary IgA binding to Lactobacillus rhamnosus and Escherichia coli, very distant bacterial species, as well as to isolated bacterial components (r =.70–.97). The binding of total salivary IgA resembled the binding of both salivary IgA1 and IgA2, with IgA2 predominating. For serum polyclonal IgA repertoire, substantially higher specificity was obtained. Serum IgA binding to E. coli correlated best with serum IgA binding to lipopolysaccharide (r =.86), and serum IgA against L. rhamnosus correlated best with the anti-peptidoglycan IgA levels (r =.88). We have also detected that total serum IgA response is governed by either IgA1 or IgA2 response, depending on the nature of the antigen/s. We conclude that steady state salivary IgA repertoire, unlike serum IgA repertoire, consists of polyreactive antibodies with innate specificity, questioning its capacity to select resident microbiota.",
publisher = "Wiley-Blackwell",
journal = "Scandinavian Journal of Immunology",
title = "Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans",
number = "6",
pages = "e13223",
volume = "96",
doi = "10.1111/sji.13223"
}

Nikodijević, S., Blagojević, V., Ćuruvija, I., Kosanović, D., Đukić, T., Đorđević, B., Ilić, V.,& Minić, R.. (2022). Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology
Wiley-Blackwell., 96(6), e13223.
https://doi.org/10.1111/sji.13223

Nikodijević S, Blagojević V, Ćuruvija I, Kosanović D, Đukić T, Đorđević B, Ilić V, Minić R. Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans. in Scandinavian Journal of Immunology. 2022;96(6):e13223.
doi:10.1111/sji.13223 .

Nikodijević, Slavomir, Blagojević, Veljko, Ćuruvija, Ivana, Kosanović, Dejana, Đukić, Tamara, Đorđević, Brižita, Ilić, Vesna, Minić, Rajna, "Selectivity of polyclonal repertoire of anti‐microbial IgA and its subclasses in saliva and serum in humans" in Scandinavian Journal of Immunology, 96, no. 6 (2022):e13223,
https://doi.org/10.1111/sji.13223 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Maslovarić, Irina
AU  - Trivanović, Drenka
AU  - Ilić, Vesna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1274
AB  - Hemoglobin is essential for maintaining cellular bioenergetic homeostasis through its ability to bind and transport oxygen to the tissues. Besides its ability to transport oxygen, hemoglobin within erythrocytes plays an important role in cellular signaling and modulation of the inflammatory response either directly by binding gas molecules (NO, CO, and CO2) or indirectly by acting as their source. Once hemoglobin reaches the extracellular environment, it acquires several secondary functions affecting surrounding cells and tissues. By modulating the cell functions, this macromolecule becomes involved in the etiology and pathophysiology of various diseases. The up-to-date results disclose the impact of extracellular hemoglobin on (i) redox status, (ii) inflammatory state of cells, (iii) proliferation and chemotaxis, (iv) mitochondrial dynamic, (v) chemoresistance and (vi) differentiation. This review pays special attention to applied biomedical research and the use of non-vertebrate and vertebrate extracellular hemoglobin as a promising candidate for hemoglobin-based oxygen carriers, as well as cell culture medium additive. Although recent experimental settings have some limitations, they provide additional insight into the modulatory activity of extracellular hemoglobin in various cellular microenvironments, such as stem or tumor cells niches.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T1  - Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects
IS  - 11
SP  - 1708
VL  - 12
DO  - 10.3390/biom12111708
ER  - 

@article{
author = "Drvenica, Ivana and Stančić, Ana and Maslovarić, Irina and Trivanović, Drenka and Ilić, Vesna",
year = "2022",
abstract = "Hemoglobin is essential for maintaining cellular bioenergetic homeostasis through its ability to bind and transport oxygen to the tissues. Besides its ability to transport oxygen, hemoglobin within erythrocytes plays an important role in cellular signaling and modulation of the inflammatory response either directly by binding gas molecules (NO, CO, and CO2) or indirectly by acting as their source. Once hemoglobin reaches the extracellular environment, it acquires several secondary functions affecting surrounding cells and tissues. By modulating the cell functions, this macromolecule becomes involved in the etiology and pathophysiology of various diseases. The up-to-date results disclose the impact of extracellular hemoglobin on (i) redox status, (ii) inflammatory state of cells, (iii) proliferation and chemotaxis, (iv) mitochondrial dynamic, (v) chemoresistance and (vi) differentiation. This review pays special attention to applied biomedical research and the use of non-vertebrate and vertebrate extracellular hemoglobin as a promising candidate for hemoglobin-based oxygen carriers, as well as cell culture medium additive. Although recent experimental settings have some limitations, they provide additional insight into the modulatory activity of extracellular hemoglobin in various cellular microenvironments, such as stem or tumor cells niches.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules",
title = "Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects",
number = "11",
pages = "1708",
volume = "12",
doi = "10.3390/biom12111708"
}

Drvenica, I., Stančić, A., Maslovarić, I., Trivanović, D.,& Ilić, V.. (2022). Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 12(11), 1708.
https://doi.org/10.3390/biom12111708

Drvenica I, Stančić A, Maslovarić I, Trivanović D, Ilić V. Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects. in Biomolecules. 2022;12(11):1708.
doi:10.3390/biom12111708 .

Drvenica, Ivana, Stančić, Ana, Maslovarić, Irina, Trivanović, Drenka, Ilić, Vesna, "Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects" in Biomolecules, 12, no. 11 (2022):1708,
https://doi.org/10.3390/biom12111708 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Grujić-Milanović, Jelica
AU  - Vejnović, Branislav
AU  - Marković, Dragana
AU  - Gvozdić, Dragan
AU  - Ilić, Vesna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1237
AB  - Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.
PB  - Taylor & Francis
T2  - Italian Journal of Animal Science
T1  - Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows
EP  - 858
IS  - 1
SP  - 847
VL  - 21
DO  - 10.1080/1828051X.2022.2070034
ER  - 

@article{
author = "Drvenica, Ivana and Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Grujić-Milanović, Jelica and Vejnović, Branislav and Marković, Dragana and Gvozdić, Dragan and Ilić, Vesna",
year = "2022",
abstract = "Redox imbalance in peripartum period influences health of dairy cows and their foetus and, through the colostrum, health of new-born calves. Oxidative stress in cattle can be suppressed by dietary supplementation with natural minerals, and we investigated the effect of supplementation with organically modified clinoptilolite on redox status parameters in healthy pregnant primiparous dairy cows. Holstein cows were randomly assigned to receive daily oral drenching, with 1 L of water containing either 0 g/L (n = 14; control group) or 150 g/L of clinoptilolite (n = 17; supplemented group). Treatment lasted from 24 ± 4 days prior to parturition until 2 days postpartum (pp). Blood samples were collected on days 24 ± 4 (–24 D) and 4 ± 2 (–4 D) prior to parturition and on days 1 (+1 D), 2 (+2 D), and 7 (+7 D) pp, and colostrum were collected at 2, 12, 24 and 36 h pp. Total antioxidant capacity, lipid peroxides, and advanced oxidation protein products (AOPP) levels were determined in peripheral blood plasma, erythrocytes, and colostrum whey. The concentration of antioxidants in the peripheral blood of supplemented cows was increased by 41% and 19% on (+2 D) and (+7 D), respectively, while the concentration of lipid peroxides on (+7 D) was lowered by 57% compared with the control group. In addition, this supplementation increased erythrocyte AOPP level on (–4 D) 61%) and colostral lipid peroxides level (90%) at 24 h pp. The results of this study showed that applied short-term supplementation with clinoptilolite influences redox homeostasis and may contribute to effective adaptation of primiparous cows to redox imbalance in the peripartum period. Highlights Short-term dietary supplementation with clinoptilolite in the prepartum period modulates redox homeostasis of the dairy cows’ blood plasma. Short-term dietary supplementation with clinoptilolite contributes to adaptation of dairy cows to redox imbalance in the peripartum period. Short-term dietary supplementation with clinoptilolite increase the level of lipid peroxides in colostrum of dairy cows.",
publisher = "Taylor & Francis",
journal = "Italian Journal of Animal Science",
title = "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows",
pages = "858-847",
number = "1",
volume = "21",
doi = "10.1080/1828051X.2022.2070034"
}

Drvenica, I., Stojić, M., Fratrić, N., Kovačić, M., Grujić-Milanović, J., Vejnović, B., Marković, D., Gvozdić, D.,& Ilić, V.. (2022). Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science
Taylor & Francis., 21(1), 847-858.
https://doi.org/10.1080/1828051X.2022.2070034

Drvenica I, Stojić M, Fratrić N, Kovačić M, Grujić-Milanović J, Vejnović B, Marković D, Gvozdić D, Ilić V. Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows. in Italian Journal of Animal Science. 2022;21(1):847-858.
doi:10.1080/1828051X.2022.2070034 .

Drvenica, Ivana, Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Grujić-Milanović, Jelica, Vejnović, Branislav, Marković, Dragana, Gvozdić, Dragan, Ilić, Vesna, "Oral supplementation with organically modified clinoptilolite during prepartum period influences the redox status of peripheral blood and colostrum of primiparous dairy cows" in Italian Journal of Animal Science, 21, no. 1 (2022):847-858,
https://doi.org/10.1080/1828051X.2022.2070034 . .

TY  - CONF
AU  - Radmilović, Mihajlo
AU  - Drvenica, Ivana
AU  - Rabasović, Mihailo
AU  - Ilić, Vesna
AU  - Pavlović, Danica
AU  - Oasa, Sho
AU  - Vukojević, Vladana
AU  - Perić, Mina
AU  - Krmpot, Aleksandar
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1439
AB  - Oslikavanje eritrocita bez obeležavanja pomoću konfokalne ili epifluorescentne mikroskopije je praktično nemoguće jer se fluorescencija od hemoglobina (Hb) ne može detektovati zbog brzih neradijativnih raspada koji su dominantni u odnosu na spontanu emisiju. Sa druge strane, demonstrirana je značajna dvofotonska apsorptivnost Hb u infracrvenoj oblasti1 kao i dvofotonski pobuđena fluorescencija (TPEF) od novoformiranog fotoprodukta2 nakon interakcije ultrakratkih laserskih impulsa sa Hb. TPEF mikroskopija je korišćena u brojnim studijama za oslikavanje Hb i eritrocita bez obeležavanja.3 U ovom radu prikazane su fotofizičke osobine i potencijalne primene novoformiranog fotoprodukta. Kreirane su fluorescentne mikrostrukture pomoću direktnog laserskog upisivanja na tankim Hb slojevima (micropatterning). Fotoprodukt je okarakterisan pomoću različitih (mikro)spektroskopskih tehnika: emisije nakon vofotonske apsorpcije, UV-VIS apsorpcije i spektralnog oslikavanja. Razmatrane su spektroskopske osobine protoporfirina IX (PpIX) pošto je on strukturni deo Hb i potencijalno može biti prekursor za formiranje fotoprodukta. Takođe, Hb je tretiran vodonik peroksidom da bi se uočila optička i spektroskopska sličnost tako dobijenog jedinjenja i fotoprodukta. Potvrđena je dugovremena fotostabilnost fotoprodukta u periodima od nekoliko meseci. Na osnovu toga što je fotoprodukt moguće dobiti na prostorno i vremenski kontrolisan način, izvršeno je fotoobeležavanje i praćenje kretanja pojedinačnih eritrocita u humanoj punoj krvi zdravog donora.
PB  - Beograd: Srpsko biološko društvo
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija
T1  - Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita
EP  - 19
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1439
ER  - 

@conference{
author = "Radmilović, Mihajlo and Drvenica, Ivana and Rabasović, Mihailo and Ilić, Vesna and Pavlović, Danica and Oasa, Sho and Vukojević, Vladana and Perić, Mina and Krmpot, Aleksandar",
year = "2022",
abstract = "Oslikavanje eritrocita bez obeležavanja pomoću konfokalne ili epifluorescentne mikroskopije je praktično nemoguće jer se fluorescencija od hemoglobina (Hb) ne može detektovati zbog brzih neradijativnih raspada koji su dominantni u odnosu na spontanu emisiju. Sa druge strane, demonstrirana je značajna dvofotonska apsorptivnost Hb u infracrvenoj oblasti1 kao i dvofotonski pobuđena fluorescencija (TPEF) od novoformiranog fotoprodukta2 nakon interakcije ultrakratkih laserskih impulsa sa Hb. TPEF mikroskopija je korišćena u brojnim studijama za oslikavanje Hb i eritrocita bez obeležavanja.3 U ovom radu prikazane su fotofizičke osobine i potencijalne primene novoformiranog fotoprodukta. Kreirane su fluorescentne mikrostrukture pomoću direktnog laserskog upisivanja na tankim Hb slojevima (micropatterning). Fotoprodukt je okarakterisan pomoću različitih (mikro)spektroskopskih tehnika: emisije nakon vofotonske apsorpcije, UV-VIS apsorpcije i spektralnog oslikavanja. Razmatrane su spektroskopske osobine protoporfirina IX (PpIX) pošto je on strukturni deo Hb i potencijalno može biti prekursor za formiranje fotoprodukta. Takođe, Hb je tretiran vodonik peroksidom da bi se uočila optička i spektroskopska sličnost tako dobijenog jedinjenja i fotoprodukta. Potvrđena je dugovremena fotostabilnost fotoprodukta u periodima od nekoliko meseci. Na osnovu toga što je fotoprodukt moguće dobiti na prostorno i vremenski kontrolisan način, izvršeno je fotoobeležavanje i praćenje kretanja pojedinačnih eritrocita u humanoj punoj krvi zdravog donora.",
publisher = "Beograd: Srpsko biološko društvo",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija",
title = "Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1439"
}

Radmilović, M., Drvenica, I., Rabasović, M., Ilić, V., Pavlović, D., Oasa, S., Vukojević, V., Perić, M.,& Krmpot, A.. (2022). Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija
Beograd: Srpsko biološko društvo., 19-19.
https://hdl.handle.net/21.15107/rcub_rimi_1439

Radmilović M, Drvenica I, Rabasović M, Ilić V, Pavlović D, Oasa S, Vukojević V, Perić M, Krmpot A. Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija. 2022;:19-19.
https://hdl.handle.net/21.15107/rcub_rimi_1439 .

Radmilović, Mihajlo, Drvenica, Ivana, Rabasović, Mihailo, Ilić, Vesna, Pavlović, Danica, Oasa, Sho, Vukojević, Vladana, Perić, Mina, Krmpot, Aleksandar, "Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija (2022):19-19,
https://hdl.handle.net/21.15107/rcub_rimi_1439 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Bugarski, Branko
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1187
AB  - Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.
PB  - MDPI
T2  - Processes
T1  - Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin
IS  - 1
SP  - 32
VL  - 10
DO  - 10.3390/pr10010032
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Ilić, Vesna and Bugarski, Branko and Bugarski, Diana",
year = "2022",
abstract = "Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.",
publisher = "MDPI",
journal = "Processes",
title = "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin",
number = "1",
pages = "32",
volume = "10",
doi = "10.3390/pr10010032"
}

Stančić, A., Drvenica, I., Ilić, V., Bugarski, B.,& Bugarski, D.. (2022). Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes
MDPI., 10(1), 32.
https://doi.org/10.3390/pr10010032

Stančić A, Drvenica I, Ilić V, Bugarski B, Bugarski D. Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes. 2022;10(1):32.
doi:10.3390/pr10010032 .

Stančić, Ana, Drvenica, Ivana, Ilić, Vesna, Bugarski, Branko, Bugarski, Diana, "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin" in Processes, 10, no. 1 (2022):32,
https://doi.org/10.3390/pr10010032 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Mojsilović, Slavko
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Saso, Luciano
AU  - Nicoletti, Marcello
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1086
AB  - Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration  gt = 500 mu g/mL. The antioxidant activity of hennosides (concentration  gt = 100 mu g/mL) was determined by FRAP and ABTS assays. At concentration of 500 mu g/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 +/- 0.08, 0.62 +/- 0.28 and 0.35 +/- 0.03 mM FeSO4 x 7H(2)O, and 0.15 +/- 0.01, 0.30 +/- 0.01 and 0.09 +/- 0.01 mM Trolox. Hennosides at 100 mu g/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.
PB  - MDPI
T2  - Plants-Basel
T1  - Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead
IS  - 2
SP  - 237
VL  - 10
DO  - 10.3390/plants10020237
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Drvenica, Ivana and Stančić, Ana and Mojsilović, Slavko and Kukolj, Tamara and Bugarski, Diana and Saso, Luciano and Nicoletti, Marcello",
year = "2021",
abstract = "Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration  gt = 500 mu g/mL. The antioxidant activity of hennosides (concentration  gt = 100 mu g/mL) was determined by FRAP and ABTS assays. At concentration of 500 mu g/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 +/- 0.08, 0.62 +/- 0.28 and 0.35 +/- 0.03 mM FeSO4 x 7H(2)O, and 0.15 +/- 0.01, 0.30 +/- 0.01 and 0.09 +/- 0.01 mM Trolox. Hennosides at 100 mu g/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.",
publisher = "MDPI",
journal = "Plants-Basel",
title = "Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead",
number = "2",
pages = "237",
volume = "10",
doi = "10.3390/plants10020237"
}

Maslovarić, I., Ilić, V., Drvenica, I., Stančić, A., Mojsilović, S., Kukolj, T., Bugarski, D., Saso, L.,& Nicoletti, M.. (2021). Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. in Plants-Basel
MDPI., 10(2), 237.
https://doi.org/10.3390/plants10020237

Maslovarić I, Ilić V, Drvenica I, Stančić A, Mojsilović S, Kukolj T, Bugarski D, Saso L, Nicoletti M. Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. in Plants-Basel. 2021;10(2):237.
doi:10.3390/plants10020237 .

Maslovarić, Irina, Ilić, Vesna, Drvenica, Ivana, Stančić, Ana, Mojsilović, Slavko, Kukolj, Tamara, Bugarski, Diana, Saso, Luciano, Nicoletti, Marcello, "Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead" in Plants-Basel, 10, no. 2 (2021):237,
https://doi.org/10.3390/plants10020237 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Stančić, Ana
AU  - Marković, Dragana
AU  - Kovačić, Marijana
AU  - Maslovarić, Irina
AU  - Rapajić, Ivana
AU  - Vučetić, Dušan
AU  - Ilić, Vesna
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1176
AB  - Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.
PB  - Springer Nature
T2  - European Biophysics Journal
T1  - The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method
EP  - 846
IS  - 6
SP  - 829
VL  - 50
DO  - 10.1007/s00249-021-01527-3
ER  - 

@article{
author = "Drvenica, Ivana and Mojsilović, Slavko and Stančić, Ana and Marković, Dragana and Kovačić, Marijana and Maslovarić, Irina and Rapajić, Ivana and Vučetić, Dušan and Ilić, Vesna",
year = "2021",
abstract = "Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.",
publisher = "Springer Nature",
journal = "European Biophysics Journal",
title = "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method",
pages = "846-829",
number = "6",
volume = "50",
doi = "10.1007/s00249-021-01527-3"
}

Drvenica, I., Mojsilović, S., Stančić, A., Marković, D., Kovačić, M., Maslovarić, I., Rapajić, I., Vučetić, D.,& Ilić, V.. (2021). The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal
Springer Nature., 50(6), 829-846.
https://doi.org/10.1007/s00249-021-01527-3

Drvenica I, Mojsilović S, Stančić A, Marković D, Kovačić M, Maslovarić I, Rapajić I, Vučetić D, Ilić V. The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal. 2021;50(6):829-846.
doi:10.1007/s00249-021-01527-3 .

Drvenica, Ivana, Mojsilović, Slavko, Stančić, Ana, Marković, Dragana, Kovačić, Marijana, Maslovarić, Irina, Rapajić, Ivana, Vučetić, Dušan, Ilić, Vesna, "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method" in European Biophysics Journal, 50, no. 6 (2021):829-846,
https://doi.org/10.1007/s00249-021-01527-3 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Santibanez, Juan F.
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1003
AB  - Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function.
AB  - Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts
T1  - Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta
EP  - 33
IS  - 1
SP  - 18
VL  - 74
DO  - 10.2298/VETGL190414019M
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Stančić, Ana and Santibanez, Juan F. and Trivanović, Drenka and Drvenica, Ivana and Krstić, Jelena and Mojsilović, Slavko and Okić Đorđević, Ivana and Bugarski, Diana",
year = "2020",
abstract = "Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function., Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts, Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta",
pages = "33-18",
number = "1",
volume = "74",
doi = "10.2298/VETGL190414019M"
}

Maslovarić, I., Ilić, V., Stančić, A., Santibanez, J. F., Trivanović, D., Drvenica, I., Krstić, J., Mojsilović, S., Okić Đorđević, I.,& Bugarski, D.. (2020). Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(1), 18-33.
https://doi.org/10.2298/VETGL190414019M

Maslovarić I, Ilić V, Stančić A, Santibanez JF, Trivanović D, Drvenica I, Krstić J, Mojsilović S, Okić Đorđević I, Bugarski D. Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik. 2020;74(1):18-33.
doi:10.2298/VETGL190414019M .

Maslovarić, Irina, Ilić, Vesna, Stančić, Ana, Santibanez, Juan F., Trivanović, Drenka, Drvenica, Ivana, Krstić, Jelena, Mojsilović, Slavko, Okić Đorđević, Ivana, Bugarski, Diana, "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts" in Veterinarski glasnik, 74, no. 1 (2020):18-33,
https://doi.org/10.2298/VETGL190414019M . .

TY  - JOUR
AU  - Kovačić, Marijana
AU  - Fratrić, Natalija
AU  - Arsić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Drvenica, Ivana
AU  - Marković, Dragana
AU  - Maslovarić, Irina
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Ilić, Vesna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1010
AB  - Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.
PB  - Elsevier Sci Ltd, Oxford
T2  - Research in Veterinary Science
T1  - Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes
EP  - 74
SP  - 63
VL  - 133
DO  - 10.1016/j.rvsc.2020.09.004
ER  - 

@article{
author = "Kovačić, Marijana and Fratrić, Natalija and Arsić, Aleksandra and Mojsilović, Slavko and Drvenica, Ivana and Marković, Dragana and Maslovarić, Irina and Grujić-Milanović, Jelica and Ivanov, Milan and Ilić, Vesna",
year = "2020",
abstract = "Calf bronchopneumonia is accompanied by increased level of circulating immune complexes (CIC), and we analysed size, and protein and lipid constituents of these CIC with an attempt to elucidate the connection between the CIC structural properties and their capacity to modulate leukocyte function. CIC of heathy calves (CICH) and calves with naturally occurring bronchopneumonia (CICD) were isolated by PEG precipitation and analysed by electrophoresis and chromatography. The predominant CIC proteins were IgG, albumin, and transferrin. Affinity isolated serum and CIC IgG coprecipitated several proteins, but only 75 and 80 kDa proteins bound CIC IgG, exclusively. 60 and 65 kDa proteins co-precipitated with CICD IgG, unlike CICH IgG. In both CICH and CICD, oleic acid-containing phospholipids predominated. In CICD, the content of oleic and vaccenic acid was higher than in CICH, while myristic, palmitic, stearic, linoleic and arachidonic acid showed lower content. Dynamic light scattering displayed difference in particle size distribution between CICH and CICD; 1280 nm large particles were present only in CICD. The effect of CICH and CICD on mononuclear cells (MNC) and granulocytes was analysed in vitro. CICH and CICD, with slight difference in intensity, stimulate MNC apoptosis, promote cell cycle arrest of unstimulated MNC, and cell cycle progression of PHA stimulated MNC. Both CIC reduced granulocyte apoptosis after 24 h while after 48 h this effect was detected for CICD only. These results indicate that structural differences of CICH and CICD might interfere with the CIC functional capacity, which we consider important for evaluation of CIC immunoregulatory function.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Research in Veterinary Science",
title = "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes",
pages = "74-63",
volume = "133",
doi = "10.1016/j.rvsc.2020.09.004"
}

Kovačić, M., Fratrić, N., Arsić, A., Mojsilović, S., Drvenica, I., Marković, D., Maslovarić, I., Grujić-Milanović, J., Ivanov, M.,& Ilić, V.. (2020). Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science
Elsevier Sci Ltd, Oxford., 133, 63-74.
https://doi.org/10.1016/j.rvsc.2020.09.004

Kovačić M, Fratrić N, Arsić A, Mojsilović S, Drvenica I, Marković D, Maslovarić I, Grujić-Milanović J, Ivanov M, Ilić V. Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes. in Research in Veterinary Science. 2020;133:63-74.
doi:10.1016/j.rvsc.2020.09.004 .

Kovačić, Marijana, Fratrić, Natalija, Arsić, Aleksandra, Mojsilović, Slavko, Drvenica, Ivana, Marković, Dragana, Maslovarić, Irina, Grujić-Milanović, Jelica, Ivanov, Milan, Ilić, Vesna, "Structural characteristics of circulating immune complexes in calves with bronchopneumonia: Impact on the quiescent leukocytes" in Research in Veterinary Science, 133 (2020):63-74,
https://doi.org/10.1016/j.rvsc.2020.09.004 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/983
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić V, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1038
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić V, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - CONF
AU  - Dragacević, Luka
AU  - Minić, Rajna
AU  - Michalickova, Danica
AU  - Ilić, Vesna
AU  - Đorđević, Brižita
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1022
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults
EP  - S18
SP  - S18
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1022
ER  - 

@conference{
author = "Dragacević, Luka and Minić, Rajna and Michalickova, Danica and Ilić, Vesna and Đorđević, Brižita",
year = "2020",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults",
pages = "S18-S18",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1022"
}

Dragacević, L., Minić, R., Michalickova, D., Ilić, V.,& Đorđević, B.. (2020). Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 54, S18-S18.
https://hdl.handle.net/21.15107/rcub_rimi_1022

Dragacević L, Minić R, Michalickova D, Ilić V, Đorđević B. Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults. in Journal of Clinical Gastroenterology. 2020;54:S18-S18.
https://hdl.handle.net/21.15107/rcub_rimi_1022 .

Dragacević, Luka, Minić, Rajna, Michalickova, Danica, Ilić, Vesna, Đorđević, Brižita, "Comparison of Specific IGG and IGA Subclass Levels to Lactobacillus and Streptococcus in Young Healthy Adults" in Journal of Clinical Gastroenterology, 54 (2020):S18-S18,
https://hdl.handle.net/21.15107/rcub_rimi_1022 .

TY  - CONF
AU  - Dragacević, Luka
AU  - Đorđević, Brižita
AU  - Gavrović-Jankulović, Marija
AU  - Ilić, Vesna
AU  - Minić, Rajna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1025
PB  - Lippincott Williams & Wilkins, Philadelphia
C3  - Journal of Clinical Gastroenterology
T1  - Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa
EP  - S13
SP  - S13
VL  - 54
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1025
ER  - 

@conference{
author = "Dragacević, Luka and Đorđević, Brižita and Gavrović-Jankulović, Marija and Ilić, Vesna and Minić, Rajna",
year = "2020",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Clinical Gastroenterology",
title = "Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa",
pages = "S13-S13",
volume = "54",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1025"
}

Dragacević, L., Đorđević, B., Gavrović-Jankulović, M., Ilić, V.,& Minić, R.. (2020). Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa. in Journal of Clinical Gastroenterology
Lippincott Williams & Wilkins, Philadelphia., 54, S13-S13.
https://hdl.handle.net/21.15107/rcub_rimi_1025

Dragacević L, Đorđević B, Gavrović-Jankulović M, Ilić V, Minić R. Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa. in Journal of Clinical Gastroenterology. 2020;54:S13-S13.
https://hdl.handle.net/21.15107/rcub_rimi_1025 .

Dragacević, Luka, Đorđević, Brižita, Gavrović-Jankulović, Marija, Ilić, Vesna, Minić, Rajna, "Typing of Surface Glycosilation of Microorganisms by Lectins with in House Elisa" in Journal of Clinical Gastroenterology, 54 (2020):S13-S13,
https://hdl.handle.net/21.15107/rcub_rimi_1025 .

TY  - JOUR
AU  - Dragačević, Luka
AU  - Đorđević, Brižita
AU  - Gavrović-Jankulović, Marija
AU  - Ilić, Vesna
AU  - Kanazir, Danijela
AU  - Minić, Rajna
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1035
AB  - The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA(120) - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA(120) which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ss-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ss-glucan from yeast with IC50 1.81 mu g mL(-1) and to P. roqueforti with 1.10 mu g mL(-1). This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ss-glucan in solutions.
PB  - Springer, Dordrecht
T2  - Glycoconjugate Journal
T1  - ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin
EP  - 105
IS  - 1
SP  - 95
VL  - 37
DO  - 10.1007/s10719-019-09898-8
ER  - 

@article{
author = "Dragačević, Luka and Đorđević, Brižita and Gavrović-Jankulović, Marija and Ilić, Vesna and Kanazir, Danijela and Minić, Rajna",
year = "2020",
abstract = "The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host's cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA(120) - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms' surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA(120) which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ss-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ss-glucan from yeast with IC50 1.81 mu g mL(-1) and to P. roqueforti with 1.10 mu g mL(-1). This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ss-glucan in solutions.",
publisher = "Springer, Dordrecht",
journal = "Glycoconjugate Journal",
title = "ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin",
pages = "105-95",
number = "1",
volume = "37",
doi = "10.1007/s10719-019-09898-8"
}

Dragačević, L., Đorđević, B., Gavrović-Jankulović, M., Ilić, V., Kanazir, D.,& Minić, R.. (2020). ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin. in Glycoconjugate Journal
Springer, Dordrecht., 37(1), 95-105.
https://doi.org/10.1007/s10719-019-09898-8

Dragačević L, Đorđević B, Gavrović-Jankulović M, Ilić V, Kanazir D, Minić R. ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin. in Glycoconjugate Journal. 2020;37(1):95-105.
doi:10.1007/s10719-019-09898-8 .

Dragačević, Luka, Đorđević, Brižita, Gavrović-Jankulović, Marija, Ilić, Vesna, Kanazir, Danijela, Minić, Rajna, "ELLSA based profiling of surface glycosylation in microorganisms reveals that ss-glucan rich yeasts' surfaces are selectively recognized with recombinant banana lectin" in Glycoconjugate Journal, 37, no. 1 (2020):95-105,
https://doi.org/10.1007/s10719-019-09898-8 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Ilić, Vesna
AU  - Kovačić, Marijana
AU  - Gvozdić, Dragan
AU  - Stajković, Silvana
AU  - Vejnović, Branislav
AU  - Savić, Olivera
AU  - Fratrić, Natalija
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1017
AB  - This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows
EP  - 435
IS  - 4
SP  - 429
VL  - 87
DO  - 10.1017/S0022029920001077
ER  - 

@article{
author = "Stojić, Milica and Ilić, Vesna and Kovačić, Marijana and Gvozdić, Dragan and Stajković, Silvana and Vejnović, Branislav and Savić, Olivera and Fratrić, Natalija",
year = "2020",
abstract = "This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows",
pages = "435-429",
number = "4",
volume = "87",
doi = "10.1017/S0022029920001077"
}

Stojić, M., Ilić, V., Kovačić, M., Gvozdić, D., Stajković, S., Vejnović, B., Savić, O.,& Fratrić, N.. (2020). Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research
Cambridge Univ Press, New York., 87(4), 429-435.
https://doi.org/10.1017/S0022029920001077

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research. 2020;87(4):429-435.
doi:10.1017/S0022029920001077 .

Stojić, Milica, Ilić, Vesna, Kovačić, Marijana, Gvozdić, Dragan, Stajković, Silvana, Vejnović, Branislav, Savić, Olivera, Fratrić, Natalija, "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows" in Journal of Dairy Research, 87, no. 4 (2020):429-435,
https://doi.org/10.1017/S0022029920001077 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić, Ivana
AU  - Kukolj, Tamara
AU  - Ilić, Vesna
AU  - Jauković, Aleksandra
AU  - Terzić, Milan
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/969
AB  - Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.
PB  - W B Saunders Co Ltd, London
T2  - Placenta
T1  - Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche
EP  - 34
SP  - 25
VL  - 82
DO  - 10.1016/j.placenta.2019.05.005
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Trivanović, Drenka and Mojsilović, Slavko and Okić, Ivana and Kukolj, Tamara and Ilić, Vesna and Jauković, Aleksandra and Terzić, Milan and Bugarski, Diana",
year = "2019",
abstract = "Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.",
publisher = "W B Saunders Co Ltd, London",
journal = "Placenta",
title = "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche",
pages = "34-25",
volume = "82",
doi = "10.1016/j.placenta.2019.05.005"
}

Obradović, H., Krstić, J., Trivanović, D., Mojsilović, S., Okić, I., Kukolj, T., Ilić, V., Jauković, A., Terzić, M.,& Bugarski, D.. (2019). Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta
W B Saunders Co Ltd, London., 82, 25-34.
https://doi.org/10.1016/j.placenta.2019.05.005

Obradović H, Krstić J, Trivanović D, Mojsilović S, Okić I, Kukolj T, Ilić V, Jauković A, Terzić M, Bugarski D. Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta. 2019;82:25-34.
doi:10.1016/j.placenta.2019.05.005 .

Obradović, Hristina, Krstić, Jelena, Trivanović, Drenka, Mojsilović, Slavko, Okić, Ivana, Kukolj, Tamara, Ilić, Vesna, Jauković, Aleksandra, Terzić, Milan, Bugarski, Diana, "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche" in Placenta, 82 (2019):25-34,
https://doi.org/10.1016/j.placenta.2019.05.005 . .

TY  - CHAP
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Bugarski, Branko
AU  - Pajić-Lijaković, Ivana
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/920
AB  - For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.
T2  - Erythrocytes: Structure, Functions & Clinical Aspects
T1  - Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems
EP  - 132
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4194
ER  - 

@inbook{
author = "Drvenica, Ivana and Stančić, Ana and Bugarski, Branko and Pajić-Lijaković, Ivana and Maslovarić, Irina and Ilić, Vesna",
year = "2019",
abstract = "For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.",
journal = "Erythrocytes: Structure, Functions & Clinical Aspects",
booktitle = "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems",
pages = "132-57",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4194"
}

Drvenica, I., Stančić, A., Bugarski, B., Pajić-Lijaković, I., Maslovarić, I.,& Ilić, V.. (2019). Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions & Clinical Aspects, 57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194

Drvenica I, Stančić A, Bugarski B, Pajić-Lijaković I, Maslovarić I, Ilić V. Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions & Clinical Aspects. 2019;:57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

Drvenica, Ivana, Stančić, Ana, Bugarski, Branko, Pajić-Lijaković, Ivana, Maslovarić, Irina, Ilić, Vesna, "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems" in Erythrocytes: Structure, Functions & Clinical Aspects (2019):57-132,
https://hdl.handle.net/21.15107/rcub_technorep_4194 .