TY  - CONF
AU  - Ristić, Biljana
AU  - Trpkov, Đorđe
AU  - Drvenica, Ivana
AU  - Dojčilović, Radovan
AU  - Đukić, Tamara
AU  - Tošić, Dragana
AU  - Pajović, Jelena
AU  - Božanić, Dušan K.
AU  - Trivanović, Drenka
AU  - Matić, Tamara
AU  - Sredojević, Dušan
AU  - Ilić, Vesna
AU  - Đoković, Vladimir
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1471
AB  - Nitrogen-doped carbon quantum dots (N-CQDs) are promising next
generation nanomaterials for potential biomedical applications such as bioimaging, biosensing,
and drug/gene delivery. However, N-CQDs biocompatibility has not been extensively
investigated. Here, we report physico-chemical characteristics of newly synthetized N-CQDs
and their effects on red blood cells (RBC), by analyzing their hemolytic activity, impact on
RBC rheology/morphology, and oxidative stress induction. N-CQDs were prepared by
hydrothermal method using citric acid and urea as precursors. Structural analyses of as prepared
N-GQDs, observed by HRTEM/EDS, showed that the lateral dimensions of the particles are in
the 10 to 20 nm range, as well as that the carbon, oxygen, and nitrogen are present in the
nanosystem. Based on AFM measurements, the average height of N-CQDs was 3.9±0.08 Å.
Photoluminescence emission (PLE) spectrum demonstrated that N-CQDs exhibit stable and
strong fluorescence in green (520 nm) region, upon 410 nm excitation. FTIR spectroscopy
indicated vibrational bands, characteristic for carbon structures and primary amines (Ndoping).
N-CQDs were negatively charged with an average Zeta potential of -15.3
mV as confirmed by DLS. To investigate hemocompatibility of N-CQDs, the RBC, the most
abundant cells in blood, were treated with different concentration of N-CQDs (10-400 ug/ml)
for 2h. Obtained results showed that there was no hemolytic activity. Moreover, ektacytometry
analysis demonstrated that N-CQDs did not affect deformability of RBC. Fluorescent
microscopy analyses revealed that treatment with N-CQDs did not induce significant
morphological aberrant forms of RBC which was also confirmed by SEM analyses. Flow
cytometry confirmed only slight RBC morphological changes based on FSC/SSC analysis.
Furthermore, using ROS sensitive dye flow cytometry analyses suggested that N-CQDs did not
induce oxidative stress in RBC. Taken together, our findings highlighted that exposure of RBC
to N-CQDs only led to the attachment of N-CQDs on RBC membranes, but there is no other
evidence of their nanotoxicity. These findings suggested that N-CQDs synthetized from ecofriendly
precursors are potentially biocompatible and safe for biomedical application.
PB  - Belgrade : Institute of Physics
C3  - Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
T1  - Hemocompatibility evaluation of N-doped carbon quantum dots
EP  - 47
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1471
ER  - 

@conference{
author = "Ristić, Biljana and Trpkov, Đorđe and Drvenica, Ivana and Dojčilović, Radovan and Đukić, Tamara and Tošić, Dragana and Pajović, Jelena and Božanić, Dušan K. and Trivanović, Drenka and Matić, Tamara and Sredojević, Dušan and Ilić, Vesna and Đoković, Vladimir",
year = "2024",
abstract = "Nitrogen-doped carbon quantum dots (N-CQDs) are promising next
generation nanomaterials for potential biomedical applications such as bioimaging, biosensing,
and drug/gene delivery. However, N-CQDs biocompatibility has not been extensively
investigated. Here, we report physico-chemical characteristics of newly synthetized N-CQDs
and their effects on red blood cells (RBC), by analyzing their hemolytic activity, impact on
RBC rheology/morphology, and oxidative stress induction. N-CQDs were prepared by
hydrothermal method using citric acid and urea as precursors. Structural analyses of as prepared
N-GQDs, observed by HRTEM/EDS, showed that the lateral dimensions of the particles are in
the 10 to 20 nm range, as well as that the carbon, oxygen, and nitrogen are present in the
nanosystem. Based on AFM measurements, the average height of N-CQDs was 3.9±0.08 Å.
Photoluminescence emission (PLE) spectrum demonstrated that N-CQDs exhibit stable and
strong fluorescence in green (520 nm) region, upon 410 nm excitation. FTIR spectroscopy
indicated vibrational bands, characteristic for carbon structures and primary amines (Ndoping).
N-CQDs were negatively charged with an average Zeta potential of -15.3
mV as confirmed by DLS. To investigate hemocompatibility of N-CQDs, the RBC, the most
abundant cells in blood, were treated with different concentration of N-CQDs (10-400 ug/ml)
for 2h. Obtained results showed that there was no hemolytic activity. Moreover, ektacytometry
analysis demonstrated that N-CQDs did not affect deformability of RBC. Fluorescent
microscopy analyses revealed that treatment with N-CQDs did not induce significant
morphological aberrant forms of RBC which was also confirmed by SEM analyses. Flow
cytometry confirmed only slight RBC morphological changes based on FSC/SSC analysis.
Furthermore, using ROS sensitive dye flow cytometry analyses suggested that N-CQDs did not
induce oxidative stress in RBC. Taken together, our findings highlighted that exposure of RBC
to N-CQDs only led to the attachment of N-CQDs on RBC membranes, but there is no other
evidence of their nanotoxicity. These findings suggested that N-CQDs synthetized from ecofriendly
precursors are potentially biocompatible and safe for biomedical application.",
publisher = "Belgrade : Institute of Physics",
journal = "Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024",
title = "Hemocompatibility evaluation of N-doped carbon quantum dots",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1471"
}

Ristić, B., Trpkov, Đ., Drvenica, I., Dojčilović, R., Đukić, T., Tošić, D., Pajović, J., Božanić, D. K., Trivanović, D., Matić, T., Sredojević, D., Ilić, V.,& Đoković, V.. (2024). Hemocompatibility evaluation of N-doped carbon quantum dots. in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
Belgrade : Institute of Physics., 47-47.
https://hdl.handle.net/21.15107/rcub_rimi_1471

Ristić B, Trpkov Đ, Drvenica I, Dojčilović R, Đukić T, Tošić D, Pajović J, Božanić DK, Trivanović D, Matić T, Sredojević D, Ilić V, Đoković V. Hemocompatibility evaluation of N-doped carbon quantum dots. in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024. 2024;:47-47.
https://hdl.handle.net/21.15107/rcub_rimi_1471 .

Ristić, Biljana, Trpkov, Đorđe, Drvenica, Ivana, Dojčilović, Radovan, Đukić, Tamara, Tošić, Dragana, Pajović, Jelena, Božanić, Dušan K., Trivanović, Drenka, Matić, Tamara, Sredojević, Dušan, Ilić, Vesna, Đoković, Vladimir, "Hemocompatibility evaluation of N-doped carbon quantum dots" in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 (2024):47-47,
https://hdl.handle.net/21.15107/rcub_rimi_1471 .