TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Milanović, Slađan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Miletić, Maja
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1474
AB  - The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T1  - Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients
IS  - 6
SP  - 3138
VL  - 25
DO  - 10.3390/ijms25063138
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Milanović, Slađan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Miletić, Maja and Bugarski, Branko and Ilić, Vesna",
year = "2024",
abstract = "The function of immune complexes in rheumatoid arthritis (RA) is related to their composition and size. Using dynamic light scattering (DLS), we investigated the link between the RA circulating immune complex (CIC) particles’ size and the CIC immunoglobulin level. In this study, 30 RA patients and 30 healthy individuals were included. IgA, IgG, and IgM were found in all analyzed CICs, but more IgA and IgG were found in RA than in control CICs. In both control and RA CICs, DLS detected 50 particles that differed in size and clustered around two size groups: with a 7.5–164 nm radius and with a 342–1718 nm radius. An increased level of IgA in RA CICs, compared to control ones, was associated with more than 50% of CIC particles. In RA, compared to the control, a higher number of CICs with 28.2 nm, 531 nm, 712 nm, and 1718 nm particles and a lower number of CICs with 78.8 nm particles were detected. This particle distribution pattern did not reflect the changes in the CIC immunoglobulin level. Thus, RA elevated CIC IgA was linked with all these particles (except the 1718 nm particle), the IgM increase was linked with 43.8 nm and 712 nm particles, and the IgG increase was linked with the 712 nm particle only. This study provides the very first data on the association between CIC particles’ size, CIC immunoglobulin level, and RA. It opens the possibility that the size of CICs determined by DLS can be used as a criterion in RA diagnosis or monitoring after a large-scale study confirmation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences",
title = "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients",
number = "6",
pages = "3138",
volume = "25",
doi = "10.3390/ijms25063138"
}

Đukić, T., Drvenica, I., Kovačić, M., Milanović, S., Majerič, D., Šefik-Bukilica, M., Miletić, M., Bugarski, B.,& Ilić, V.. (2024). Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 25(6), 3138.
https://doi.org/10.3390/ijms25063138

Đukić T, Drvenica I, Kovačić M, Milanović S, Majerič D, Šefik-Bukilica M, Miletić M, Bugarski B, Ilić V. Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients. in International Journal of Molecular Sciences. 2024;25(6):3138.
doi:10.3390/ijms25063138 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Milanović, Slađan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Miletić, Maja, Bugarski, Branko, Ilić, Vesna, "Exploring the Link between Hydrodynamic Size and Immunoglobulins of Circulating Immune Complexes in Rheumatoid Arthritis Patients" in International Journal of Molecular Sciences, 25, no. 6 (2024):3138,
https://doi.org/10.3390/ijms25063138 . .

TY  - JOUR
AU  - Đukić, Tamara
AU  - Drvenica, Ivana
AU  - Kovačić, Marijana
AU  - Minić, Rajna
AU  - Vučetić, Dušan
AU  - Majerič, Dragana
AU  - Šefik-Bukilica, Mirjana
AU  - Savić, Olivera
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1314
AB  - The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.
PB  - Elsevier
T2  - Analytical Biochemistry
T2  - Analytical BiochemistryAnalytical Biochemistry
T1  - Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients
SP  - 115194
VL  - 674
DO  - 10.1016/j.ab.2023.115194
ER  - 

@article{
author = "Đukić, Tamara and Drvenica, Ivana and Kovačić, Marijana and Minić, Rajna and Vučetić, Dušan and Majerič, Dragana and Šefik-Bukilica, Mirjana and Savić, Olivera and Bugarski, Branko and Ilić, Vesna",
year = "2023",
abstract = "The size of circulating immune complexes (CICs) in rheumatoid arthritis (RA) could be an emerging criterion in disease diagnosis. This study analyzed size and electrokinetic potential of CICs from RA patients, healthy young adults, and RA patients age-matched controls aiming to establish their unique CIC features. Pooled CIC of 30 RA patients, 30 young adults, and 30 RA group's age-matched controls (middle-aged and oldеr healthy adults), and in vitro IgG aggregates from pooled sera of 300 healthy volunteers were tested using dynamic light scattering (DLS). Size distribution of CIC in healthy young adults exhibited high polydispersity. RA CIC patients and their age-matched control showed distinctly narrower size distributions compared with young adults. In these groups, particles clustered around two well-defined peaks. Particles of peak 1 were 36.1 ± 6.8 nm in RA age-matched control, and 30.8 ± 4.2 nm in RA patients. Particles of peak 2 of the RA age-matched control's CIC was 251.7 ± 41.2 nm, while RA CIC contained larger particles (359.9 ± 50.5 nm). The lower zeta potential of RA CIC, compared to control, indicated a disease-related decrease in colloidal stability. DLS identified RA-specific, but also age-specific distribution of CIC size and opened possibility of becoming a method for CIC size analysis in IC-mediated diseases.",
publisher = "Elsevier",
journal = "Analytical Biochemistry, Analytical BiochemistryAnalytical Biochemistry",
title = "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients",
pages = "115194",
volume = "674",
doi = "10.1016/j.ab.2023.115194"
}

Đukić, T., Drvenica, I., Kovačić, M., Minić, R., Vučetić, D., Majerič, D., Šefik-Bukilica, M., Savić, O., Bugarski, B.,& Ilić, V.. (2023). Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry
Elsevier., 674, 115194.
https://doi.org/10.1016/j.ab.2023.115194

Đukić T, Drvenica I, Kovačić M, Minić R, Vučetić D, Majerič D, Šefik-Bukilica M, Savić O, Bugarski B, Ilić V. Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients. in Analytical Biochemistry. 2023;674:115194.
doi:10.1016/j.ab.2023.115194 .

Đukić, Tamara, Drvenica, Ivana, Kovačić, Marijana, Minić, Rajna, Vučetić, Dušan, Majerič, Dragana, Šefik-Bukilica, Mirjana, Savić, Olivera, Bugarski, Branko, Ilić, Vesna, "Dynamic light scattering analysis of immune complexes in sera of rheumatoid arthritis patients" in Analytical Biochemistry, 674 (2023):115194,
https://doi.org/10.1016/j.ab.2023.115194 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Bugarski, Branko
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1187
AB  - Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.
PB  - MDPI
T2  - Processes
T1  - Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin
IS  - 1
SP  - 32
VL  - 10
DO  - 10.3390/pr10010032
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Ilić, Vesna and Bugarski, Branko and Bugarski, Diana",
year = "2022",
abstract = "Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.",
publisher = "MDPI",
journal = "Processes",
title = "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin",
number = "1",
pages = "32",
volume = "10",
doi = "10.3390/pr10010032"
}

Stančić, A., Drvenica, I., Ilić, V., Bugarski, B.,& Bugarski, D.. (2022). Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes
MDPI., 10(1), 32.
https://doi.org/10.3390/pr10010032

Stančić A, Drvenica I, Ilić V, Bugarski B, Bugarski D. Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes. 2022;10(1):32.
doi:10.3390/pr10010032 .

Stančić, Ana, Drvenica, Ivana, Ilić, Vesna, Bugarski, Branko, Bugarski, Diana, "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin" in Processes, 10, no. 1 (2022):32,
https://doi.org/10.3390/pr10010032 . .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Živković, Jelena
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1269
AB  - The  aim  of  this  study  was  to  optimize  the  parameters  for  the  extraction  of  total  flavonoids  from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless  of  extraction  time,  aqueous  methanolicextracts  had  higher  total  flavonoid  content  than  did aqueous ethanolic extracts. So, full factorial design and response  surface  methodology (RSM)  were  em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol  content  in  the  solvent  influenced  significantly  total  flavonoid  content  and FRAP  (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous  methanol  and  67  min,  and  65%  aqueous  methanol  and  83  min  for  maximal FRAPantioxidantcapacity.  DPPH  (2,2-diphenyl-1-picrylhydrazyl)  antioxidant  capacity  was  not  significantly  affected  by extraction time or methanol percentage in the solvent.
PB  - Society of Chemists and Technologists of Macedonia and  Ss. Cyril and Methodius University in Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
EP  - 128
IS  - 1
SP  - 119
VL  - 41
DO  - 10.20450/mjcce.2022.2238
ER  - 

@article{
author = "Vajić, Una Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "The  aim  of  this  study  was  to  optimize  the  parameters  for  the  extraction  of  total  flavonoids  from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless  of  extraction  time,  aqueous  methanolicextracts  had  higher  total  flavonoid  content  than  did aqueous ethanolic extracts. So, full factorial design and response  surface  methodology (RSM)  were  em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol  content  in  the  solvent  influenced  significantly  total  flavonoid  content  and FRAP  (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous  methanol  and  67  min,  and  65%  aqueous  methanol  and  83  min  for  maximal FRAPantioxidantcapacity.  DPPH  (2,2-diphenyl-1-picrylhydrazyl)  antioxidant  capacity  was  not  significantly  affected  by extraction time or methanol percentage in the solvent.",
publisher = "Society of Chemists and Technologists of Macedonia and  Ss. Cyril and Methodius University in Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology",
pages = "128-119",
number = "1",
volume = "41",
doi = "10.20450/mjcce.2022.2238"
}

Vajić, U. J., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering
Society of Chemists and Technologists of Macedonia and  Ss. Cyril and Methodius University in Skopje., 41(1), 119-128.
https://doi.org/10.20450/mjcce.2022.2238

Vajić UJ, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128.
doi:10.20450/mjcce.2022.2238 .

Vajić, Una Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128,
https://doi.org/10.20450/mjcce.2022.2238 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Blažević, Ivica
AU  - Bošković, Perica
AU  - Bratanić, Andre
AU  - Bugarski, Branko
AU  - Bilušić, Tea
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1218
AB  - Encapsulation of sinigrin in liposomes with the proliposomal method was performed in order to evaluate the effect of this process on in vitro simulated digestion and antioxidant potential of sinigrin. The recovery of sinigrin after simulated gastric and duodenal digestion of its free and liposomal forms was determined with HPLC-UV using human digestive juices. The antioxidant potential of sinigrin and sinigrin-loaded liposomes was determined with the Rancimat test as their ability to prolong oxidative stability of edible oil. The efficiency of 62% was obtained by encapsulating sinigrin in liposomes. The values of mean diameter, polydispersity index and zeta potential showed satisfactory size uniformity and physical stability of the liposomes containing sinigrin. Liposomes were shown to inhibit the digestion of sinigrin in both human gastric and intestinal juices, clearly enabling its prolonged release. Moreover, sinigrin in the liposomal form significantly prolonged the induction time of edible oil oxidation compared to its free form. The results obtained are encouraging from the point of view of a possible incorporation of the sinigrin-loaded liposomes in real functional food systems or their use as nutraceuticals.
PB  - Polish Academy Sciences. Institute of Animal Reproduction and Food Research
T2  - Polish Journal of Food and Nutrition Sciences
T1  - Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential
EP  - 449
IS  - 4
SP  - 441
VL  - 71
DO  - 10.31883/pjfns/143574
ER  - 

@article{
author = "Drvenica, Ivana and Blažević, Ivica and Bošković, Perica and Bratanić, Andre and Bugarski, Branko and Bilušić, Tea",
year = "2021",
abstract = "Encapsulation of sinigrin in liposomes with the proliposomal method was performed in order to evaluate the effect of this process on in vitro simulated digestion and antioxidant potential of sinigrin. The recovery of sinigrin after simulated gastric and duodenal digestion of its free and liposomal forms was determined with HPLC-UV using human digestive juices. The antioxidant potential of sinigrin and sinigrin-loaded liposomes was determined with the Rancimat test as their ability to prolong oxidative stability of edible oil. The efficiency of 62% was obtained by encapsulating sinigrin in liposomes. The values of mean diameter, polydispersity index and zeta potential showed satisfactory size uniformity and physical stability of the liposomes containing sinigrin. Liposomes were shown to inhibit the digestion of sinigrin in both human gastric and intestinal juices, clearly enabling its prolonged release. Moreover, sinigrin in the liposomal form significantly prolonged the induction time of edible oil oxidation compared to its free form. The results obtained are encouraging from the point of view of a possible incorporation of the sinigrin-loaded liposomes in real functional food systems or their use as nutraceuticals.",
publisher = "Polish Academy Sciences. Institute of Animal Reproduction and Food Research",
journal = "Polish Journal of Food and Nutrition Sciences",
title = "Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential",
pages = "449-441",
number = "4",
volume = "71",
doi = "10.31883/pjfns/143574"
}

Drvenica, I., Blažević, I., Bošković, P., Bratanić, A., Bugarski, B.,& Bilušić, T.. (2021). Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential. in Polish Journal of Food and Nutrition Sciences
Polish Academy Sciences. Institute of Animal Reproduction and Food Research., 71(4), 441-449.
https://doi.org/10.31883/pjfns/143574

Drvenica I, Blažević I, Bošković P, Bratanić A, Bugarski B, Bilušić T. Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential. in Polish Journal of Food and Nutrition Sciences. 2021;71(4):441-449.
doi:10.31883/pjfns/143574 .

Drvenica, Ivana, Blažević, Ivica, Bošković, Perica, Bratanić, Andre, Bugarski, Branko, Bilušić, Tea, "Sinigrin Encapsulation in Liposomes: Influence on In Vitro Digestion and Antioxidant Potential" in Polish Journal of Food and Nutrition Sciences, 71, no. 4 (2021):441-449,
https://doi.org/10.31883/pjfns/143574 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/983
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić V, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1038
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić V, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - CHAP
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Bugarski, Branko
AU  - Pajić-Lijaković, Ivana
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/920
AB  - For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.
T2  - Erythrocytes: Structure, Functions & Clinical Aspects
T1  - Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems
EP  - 132
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4194
ER  - 

@inbook{
author = "Drvenica, Ivana and Stančić, Ana and Bugarski, Branko and Pajić-Lijaković, Ivana and Maslovarić, Irina and Ilić, Vesna",
year = "2019",
abstract = "For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.",
journal = "Erythrocytes: Structure, Functions & Clinical Aspects",
booktitle = "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems",
pages = "132-57",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4194"
}

Drvenica, I., Stančić, A., Bugarski, B., Pajić-Lijaković, I., Maslovarić, I.,& Ilić, V.. (2019). Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions & Clinical Aspects, 57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194

Drvenica I, Stančić A, Bugarski B, Pajić-Lijaković I, Maslovarić I, Ilić V. Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions & Clinical Aspects. 2019;:57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

Drvenica, Ivana, Stančić, Ana, Bugarski, Branko, Pajić-Lijaković, Ivana, Maslovarić, Irina, Ilić, Vesna, "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems" in Erythrocytes: Structure, Functions & Clinical Aspects (2019):57-132,
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Kalušević, Ana
AU  - Marković, Smilja B.
AU  - Dragišić-Maksimović, Jelena J.
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/932
AB  - Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin
EP  - 1117
IS  - 10
SP  - 1105
VL  - 84
DO  - 10.2298/JSC190513067D
ER  - 

@article{
author = "Drvenica, Ivana and Stančić, Ana and Kalušević, Ana and Marković, Smilja B. and Dragišić-Maksimović, Jelena J. and Nedović, Viktor and Bugarski, Branko and Ilić, Vesna",
year = "2019",
abstract = "Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin",
pages = "1117-1105",
number = "10",
volume = "84",
doi = "10.2298/JSC190513067D"
}

Drvenica, I., Stančić, A., Kalušević, A., Marković, S. B., Dragišić-Maksimović, J. J., Nedović, V., Bugarski, B.,& Ilić, V.. (2019). Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(10), 1105-1117.
https://doi.org/10.2298/JSC190513067D

Drvenica I, Stančić A, Kalušević A, Marković SB, Dragišić-Maksimović JJ, Nedović V, Bugarski B, Ilić V. Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society. 2019;84(10):1105-1117.
doi:10.2298/JSC190513067D .

Drvenica, Ivana, Stančić, Ana, Kalušević, Ana, Marković, Smilja B., Dragišić-Maksimović, Jelena J., Nedović, Viktor, Bugarski, Branko, Ilić, Vesna, "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin" in Journal of the Serbian Chemical Society, 84, no. 10 (2019):1105-1117,
https://doi.org/10.2298/JSC190513067D . .

TY  - JOUR
AU  - Liović, Nikolina
AU  - Bošković, Perica
AU  - Drvenica, Ivana
AU  - Jambrak, Anet Rezek
AU  - Dropulić, Ana Marija
AU  - Kresić, Greta
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Zorić, Zoran
AU  - Pedisić, Sandra
AU  - Bilusić, Tea
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/974
AB  - Natural phenolic compounds are recognized as bioactive ingredients in food but can also have a role as effective alternatives to synthetic antioxidants in stability improvement of foods prone to oxidation, such as edible oils. This study aimed at the preparation and HPLC-DAD characterization of phenolic extracts from lisccinium corymbosum L. (raw, pasteurized, freeze-dried and treated with high-intensity ultrasound), and at testing their antioxidant potential in the prevention of olive oil oxidation in the native state and encapsulated into microemulsions and liposomes systems. Water-in-oil structured microemulsions used in this study were prepared using mechanical, ultrasonic, and high pressure homogenization. Liposomes with the average size of 589.1 +/- 2.9 nm were produced with the proliposome method using commercially available phosphatidylcholine - Phospolipon 90G. The obtained results showed significant prolongation of the oxidative stability of extra virgin olive oil enriched with encapsulated blueberry phenolic extracts than with native phenolic extracts, regardless of the method used for blueberry processing. Phenolic extracts encapsulated in microemulsions had a stronger effect on the prolongation of olive oil oxidative stability in comparison with the extracts encapsulated in liposomes. The average prolongation rate of oxidative stability was 45.65% by phenolic extracts encapsulated in microemulsions prepared by mechanical homogenization (p=0.012), and 58.72% by microemulsions prepared by ultrasound homogenization (p=0.011). Phenolic extracts encapsulated in microemulsions prepared by high pressure homogenization had no effect on oil oxidative stability prolongation.
PB  - De Gruyter Poland Sp Zoo, Warsaw
T2  - Polish Journal of Food & Nutrition Sciences
T1  - Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability
EP  - 33
IS  - 1
SP  - 23
VL  - 69
DO  - 10.31883/pjfns-2019-0003
ER  - 

@article{
author = "Liović, Nikolina and Bošković, Perica and Drvenica, Ivana and Jambrak, Anet Rezek and Dropulić, Ana Marija and Kresić, Greta and Nedović, Viktor and Bugarski, Branko and Zorić, Zoran and Pedisić, Sandra and Bilusić, Tea",
year = "2019",
abstract = "Natural phenolic compounds are recognized as bioactive ingredients in food but can also have a role as effective alternatives to synthetic antioxidants in stability improvement of foods prone to oxidation, such as edible oils. This study aimed at the preparation and HPLC-DAD characterization of phenolic extracts from lisccinium corymbosum L. (raw, pasteurized, freeze-dried and treated with high-intensity ultrasound), and at testing their antioxidant potential in the prevention of olive oil oxidation in the native state and encapsulated into microemulsions and liposomes systems. Water-in-oil structured microemulsions used in this study were prepared using mechanical, ultrasonic, and high pressure homogenization. Liposomes with the average size of 589.1 +/- 2.9 nm were produced with the proliposome method using commercially available phosphatidylcholine - Phospolipon 90G. The obtained results showed significant prolongation of the oxidative stability of extra virgin olive oil enriched with encapsulated blueberry phenolic extracts than with native phenolic extracts, regardless of the method used for blueberry processing. Phenolic extracts encapsulated in microemulsions had a stronger effect on the prolongation of olive oil oxidative stability in comparison with the extracts encapsulated in liposomes. The average prolongation rate of oxidative stability was 45.65% by phenolic extracts encapsulated in microemulsions prepared by mechanical homogenization (p=0.012), and 58.72% by microemulsions prepared by ultrasound homogenization (p=0.011). Phenolic extracts encapsulated in microemulsions prepared by high pressure homogenization had no effect on oil oxidative stability prolongation.",
publisher = "De Gruyter Poland Sp Zoo, Warsaw",
journal = "Polish Journal of Food & Nutrition Sciences",
title = "Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability",
pages = "33-23",
number = "1",
volume = "69",
doi = "10.31883/pjfns-2019-0003"
}

Liović, N., Bošković, P., Drvenica, I., Jambrak, A. R., Dropulić, A. M., Kresić, G., Nedović, V., Bugarski, B., Zorić, Z., Pedisić, S.,& Bilusić, T.. (2019). Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability. in Polish Journal of Food & Nutrition Sciences
De Gruyter Poland Sp Zoo, Warsaw., 69(1), 23-33.
https://doi.org/10.31883/pjfns-2019-0003

Liović N, Bošković P, Drvenica I, Jambrak AR, Dropulić AM, Kresić G, Nedović V, Bugarski B, Zorić Z, Pedisić S, Bilusić T. Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability. in Polish Journal of Food & Nutrition Sciences. 2019;69(1):23-33.
doi:10.31883/pjfns-2019-0003 .

Liović, Nikolina, Bošković, Perica, Drvenica, Ivana, Jambrak, Anet Rezek, Dropulić, Ana Marija, Kresić, Greta, Nedović, Viktor, Bugarski, Branko, Zorić, Zoran, Pedisić, Sandra, Bilusić, Tea, "Phenolic Extracts from Vaccinium corymbosum L. Loaded in Microemulsions and Liposomes as Enhancers of Olive Oil Oxidative Stability" in Polish Journal of Food & Nutrition Sciences, 69, no. 1 (2019):23-33,
https://doi.org/10.31883/pjfns-2019-0003 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/960
AB  - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
EP  - 390
SP  - 384
VL  - 213
DO  - 10.1016/j.saa.2019.01.069
ER  - 

@article{
author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy",
pages = "390-384",
volume = "213",
doi = "10.1016/j.saa.2019.01.069"
}

Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390.
https://doi.org/10.1016/j.saa.2019.01.069

Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;213:384-390.
doi:10.1016/j.saa.2019.01.069 .

Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 213 (2019):384-390,
https://doi.org/10.1016/j.saa.2019.01.069 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Ralević, U.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/966
AB  - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
EP  - 178
SP  - 173
VL  - 216
DO  - 10.1016/j.saa.2019.03.012
ER  - 

@article{
author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy",
pages = "178-173",
volume = "216",
doi = "10.1016/j.saa.2019.03.012"
}

Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178.
https://doi.org/10.1016/j.saa.2019.03.012

Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;216:173-178.
doi:10.1016/j.saa.2019.03.012 .

Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 216 (2019):173-178,
https://doi.org/10.1016/j.saa.2019.03.012 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/835
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine & Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine & Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine & Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Savikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/846
AB  - Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Phytomedicine
T1  - Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats
EP  - 45
SP  - 39
VL  - 46
DO  - 10.1016/j.phymed.2018.04.037
ER  - 

@article{
author = "Vajić, Una Jovana and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Ivanov, Milan and Karanović, Danijela and Savikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2018",
abstract = "Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Phytomedicine",
title = "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats",
pages = "45-39",
volume = "46",
doi = "10.1016/j.phymed.2018.04.037"
}

Vajić, U. J., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Ivanov, M., Karanović, D., Savikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2018). Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 46, 39-45.
https://doi.org/10.1016/j.phymed.2018.04.037

Vajić UJ, Grujić-Milanović J, Miloradović Z, Jovović Đ, Ivanov M, Karanović D, Savikin K, Bugarski B, Mihailović-Stanojević N. Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine. 2018;46:39-45.
doi:10.1016/j.phymed.2018.04.037 .

Vajić, Una Jovana, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Ivanov, Milan, Karanović, Danijela, Savikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats" in Phytomedicine, 46 (2018):39-45,
https://doi.org/10.1016/j.phymed.2018.04.037 . .

TY  - CONF
AU  - Bošković, Perica
AU  - Mrduljas, Nikolina
AU  - Jambrak, Anet Rezek
AU  - Drvenica, Ivana
AU  - Kresić, Greta
AU  - BeluZić, Robert
AU  - Bugarski, Branko
AU  - Bilusić, Tea
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/785
PB  - Elsevier Science Bv, Amsterdam
C3  - Journal of Biotechnology
T1  - Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes
EP  - S72
SP  - S72
VL  - 256
DO  - 10.1016/j.jbiotec.2017.06.1046
ER  - 

@conference{
author = "Bošković, Perica and Mrduljas, Nikolina and Jambrak, Anet Rezek and Drvenica, Ivana and Kresić, Greta and BeluZić, Robert and Bugarski, Branko and Bilusić, Tea",
year = "2017",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes",
pages = "S72-S72",
volume = "256",
doi = "10.1016/j.jbiotec.2017.06.1046"
}

Bošković, P., Mrduljas, N., Jambrak, A. R., Drvenica, I., Kresić, G., BeluZić, R., Bugarski, B.,& Bilusić, T.. (2017). Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 256, S72-S72.
https://doi.org/10.1016/j.jbiotec.2017.06.1046

Bošković P, Mrduljas N, Jambrak AR, Drvenica I, Kresić G, BeluZić R, Bugarski B, Bilusić T. Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes. in Journal of Biotechnology. 2017;256:S72-S72.
doi:10.1016/j.jbiotec.2017.06.1046 .

Bošković, Perica, Mrduljas, Nikolina, Jambrak, Anet Rezek, Drvenica, Ivana, Kresić, Greta, BeluZić, Robert, Bugarski, Branko, Bilusić, Tea, "Enhancement of the olive oil oxidative stability by phenolics extracts from cultivated blueberry in microemulsions and in liposomes" in Journal of Biotechnology, 256 (2017):S72-S72,
https://doi.org/10.1016/j.jbiotec.2017.06.1046 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Jovanić, Svetlana
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Ilić, Vesna
AU  - Rabasović, Mihailo D.
AU  - Pantelić, Dejan
AU  - Jelenković, Branislav
AU  - Bugarski, Branko
AU  - Krmpot, Aleksandar J.
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/800
AB  - The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.
PB  - Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham
T2  - Journal of Biomedical Optics
T1  - Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy
IS  - 2
VL  - 22
DO  - 10.1117/1.JBO.22.2.026003
ER  - 

@article{
author = "Bukara, Katarina and Jovanić, Svetlana and Drvenica, Ivana and Stančić, Ana and Ilić, Vesna and Rabasović, Mihailo D. and Pantelić, Dejan and Jelenković, Branislav and Bugarski, Branko and Krmpot, Aleksandar J.",
year = "2017",
abstract = "The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.",
publisher = "Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham",
journal = "Journal of Biomedical Optics",
title = "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy",
number = "2",
volume = "22",
doi = "10.1117/1.JBO.22.2.026003"
}

Bukara, K., Jovanić, S., Drvenica, I., Stančić, A., Ilić, V., Rabasović, M. D., Pantelić, D., Jelenković, B., Bugarski, B.,& Krmpot, A. J.. (2017). Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics
Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham., 22(2).
https://doi.org/10.1117/1.JBO.22.2.026003

Bukara K, Jovanić S, Drvenica I, Stančić A, Ilić V, Rabasović MD, Pantelić D, Jelenković B, Bugarski B, Krmpot AJ. Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics. 2017;22(2).
doi:10.1117/1.JBO.22.2.026003 .

Bukara, Katarina, Jovanić, Svetlana, Drvenica, Ivana, Stančić, Ana, Ilić, Vesna, Rabasović, Mihailo D., Pantelić, Dejan, Jelenković, Branislav, Bugarski, Branko, Krmpot, Aleksandar J., "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy" in Journal of Biomedical Optics, 22, no. 2 (2017),
https://doi.org/10.1117/1.JBO.22.2.026003 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Misić, Danijela M.
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/689
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
EP  - 22
SP  - 14
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna and Stančić, Ana and Misić, Danijela M. and Vasić, Borislav Z. and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
pages = "22-14",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017"
}

Bukara, K., Drvenica, I., Ilić, V., Stančić, A., Misić, D. M., Vasić, B. Z., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić V, Stančić A, Misić DM, Vasić BZ, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna, Stančić, Ana, Misić, Danijela M., Vasić, Borislav Z., Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Balanc, Bojana
AU  - Kalušević, Ana
AU  - Drvenica, Ivana
AU  - Coelho, Maria Teresa
AU  - Đorđević, Verica
AU  - Alves, Vitor D.
AU  - Sousa, Isabel
AU  - Moldao-Martins, Margarida
AU  - Rakić, Vesna
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/720
AB  - Carqueja (Pterospartum tridentatum) is an endemic species and various bioactive compounds have been identified in its aqueous extract. The aim of this study was to protect the natural antioxidants from the aqueous extract of carqueja by encapsulation in Ca-alginate microbeads and Ca-alginate microbeads containing 10% and 20% (w/v) of inulin. The microbeads produced by electrostatic extrusion technique had an average diameter from 625 mu m to 830 mu m depending on the portion of inulin. The sphericity factor of the hydrogel microbeads had values between 0.014 and 0.026, while freeze dried microbeads had irregular shape, especially those with no excipient. The reduction in microbeads size after freeze drying process (expressed as shrinkage factor) ranged from 0.338 (alginate microbeads with 20% (w/v) of inulin) to 0.523 (plain alginate microbeads). The expressed radical scavenging activity against ABTS and DPPH radicals was found to be between 30% and 40% for encapsulated extract, while the fresh extract showed around 47% and 57% of radical scavenging activity for ABTS and DPPH radicals, respectively. The correlation between antioxidant activity and the total phenolic content were found to be positive (in both assay methods, DPPH and ABTS), which indicate that the addition of inulin didn't have influence on antioxidant activity. The presence of inulin reduced stiffness of the hydrogel, and protected bead structure from collapse upon freeze-drying. Alginate-inulin beads are envisaged to be used for delivery of aqueous P. tridentatum extract in functional food products.
PB  - Wiley, Hoboken
T2  - Journal of Food Science
T1  - Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract
EP  - E75
IS  - 1
SP  - E65
VL  - 81
DO  - 10.1111/1750-3841.13167
ER  - 

@article{
author = "Balanc, Bojana and Kalušević, Ana and Drvenica, Ivana and Coelho, Maria Teresa and Đorđević, Verica and Alves, Vitor D. and Sousa, Isabel and Moldao-Martins, Margarida and Rakić, Vesna and Nedović, Viktor and Bugarski, Branko",
year = "2016",
abstract = "Carqueja (Pterospartum tridentatum) is an endemic species and various bioactive compounds have been identified in its aqueous extract. The aim of this study was to protect the natural antioxidants from the aqueous extract of carqueja by encapsulation in Ca-alginate microbeads and Ca-alginate microbeads containing 10% and 20% (w/v) of inulin. The microbeads produced by electrostatic extrusion technique had an average diameter from 625 mu m to 830 mu m depending on the portion of inulin. The sphericity factor of the hydrogel microbeads had values between 0.014 and 0.026, while freeze dried microbeads had irregular shape, especially those with no excipient. The reduction in microbeads size after freeze drying process (expressed as shrinkage factor) ranged from 0.338 (alginate microbeads with 20% (w/v) of inulin) to 0.523 (plain alginate microbeads). The expressed radical scavenging activity against ABTS and DPPH radicals was found to be between 30% and 40% for encapsulated extract, while the fresh extract showed around 47% and 57% of radical scavenging activity for ABTS and DPPH radicals, respectively. The correlation between antioxidant activity and the total phenolic content were found to be positive (in both assay methods, DPPH and ABTS), which indicate that the addition of inulin didn't have influence on antioxidant activity. The presence of inulin reduced stiffness of the hydrogel, and protected bead structure from collapse upon freeze-drying. Alginate-inulin beads are envisaged to be used for delivery of aqueous P. tridentatum extract in functional food products.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Science",
title = "Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract",
pages = "E75-E65",
number = "1",
volume = "81",
doi = "10.1111/1750-3841.13167"
}

Balanc, B., Kalušević, A., Drvenica, I., Coelho, M. T., Đorđević, V., Alves, V. D., Sousa, I., Moldao-Martins, M., Rakić, V., Nedović, V.,& Bugarski, B.. (2016). Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract. in Journal of Food Science
Wiley, Hoboken., 81(1), E65-E75.
https://doi.org/10.1111/1750-3841.13167

Balanc B, Kalušević A, Drvenica I, Coelho MT, Đorđević V, Alves VD, Sousa I, Moldao-Martins M, Rakić V, Nedović V, Bugarski B. Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract. in Journal of Food Science. 2016;81(1):E65-E75.
doi:10.1111/1750-3841.13167 .

Balanc, Bojana, Kalušević, Ana, Drvenica, Ivana, Coelho, Maria Teresa, Đorđević, Verica, Alves, Vitor D., Sousa, Isabel, Moldao-Martins, Margarida, Rakić, Vesna, Nedović, Viktor, Bugarski, Branko, "Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract" in Journal of Food Science, 81, no. 1 (2016):E65-E75,
https://doi.org/10.1111/1750-3841.13167 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Bukara, Katarina
AU  - Ilić, Vesna
AU  - Misić, Danijela M.
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš
AU  - Đorđević Verica B.
AU  - Veljović, Đorđe N.
AU  - Belić, Aleksandar
AU  - Bugarski, Branko
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/736
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
EP  - 1055
IS  - 4
SP  - 1046
VL  - 32
DO  - 10.1002/btpr.2304
ER  - 

@article{
author = "Drvenica, Ivana and Bukara, Katarina and Ilić, Vesna and Misić, Danijela M. and Vasić, Borislav Z. and Gajić, Radoš and Đorđević Verica B. and Veljović, Đorđe N. and Belić, Aleksandar and Bugarski, Branko",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
pages = "1055-1046",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304"
}

Drvenica, I., Bukara, K., Ilić, V., Misić, D. M., Vasić, B. Z., Gajić, R., Đorđević Verica B., Veljović, Đ. N., Belić, A.,& Bugarski, B.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress
Wiley, Hoboken., 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica I, Bukara K, Ilić V, Misić DM, Vasić BZ, Gajić R, Đorđević Verica B., Veljović ĐN, Belić A, Bugarski B. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana, Bukara, Katarina, Ilić, Vesna, Misić, Danijela M., Vasić, Borislav Z., Gajić, Radoš, Đorđević Verica B., Veljović, Đorđe N., Belić, Aleksandar, Bugarski, Branko, "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .

TY  - JOUR
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Bugarski, Branko
AU  - Jovović, Đurđica
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Komes, Draženka
AU  - Grujić-Milanović, Jelica
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/694
AB  - High blood pressure is the most powerful contributor to the cardiovascular morbidity and mortality, and inverse correlation between consumption of polyphenol-rich foods or beverages and incidence of cardiovascular diseases gains more importance. Reactive oxygen species plays an important role in the development of hypertension. We found that wild thyme (a spice plant, rich in polyphenolic compounds) induced a significant decrease of blood pressure and vascular resistance in hypertensive rats. The inverse correlation between vascular resistance and plasma heme oxygenase-1 suggests that endogenous vasodilator carbon monoxide generated by heme oxidation could account for this normalization of blood pressure. Next product of heme oxidation, bilirubin (a chain-breaking antioxidant that acts as a lipid peroxyl radical scavenger), becomes significantly increased after wild thyme treatment and induces the reduction of plasma lipid peroxidation in hypertensive, but not in normotensive rats. The obtained results promote wild thyme as useful supplement for cardiovascular interventions.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine & Cellular Longevity
T1  - Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress
VL  - 2016
DO  - 10.1155/2016/1458793
ER  - 

@article{
author = "Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Bugarski, Branko and Jovović, Đurđica and Karanović, Danijela and Vajić, Una Jovana and Komes, Draženka and Grujić-Milanović, Jelica",
year = "2016",
abstract = "High blood pressure is the most powerful contributor to the cardiovascular morbidity and mortality, and inverse correlation between consumption of polyphenol-rich foods or beverages and incidence of cardiovascular diseases gains more importance. Reactive oxygen species plays an important role in the development of hypertension. We found that wild thyme (a spice plant, rich in polyphenolic compounds) induced a significant decrease of blood pressure and vascular resistance in hypertensive rats. The inverse correlation between vascular resistance and plasma heme oxygenase-1 suggests that endogenous vasodilator carbon monoxide generated by heme oxidation could account for this normalization of blood pressure. Next product of heme oxidation, bilirubin (a chain-breaking antioxidant that acts as a lipid peroxyl radical scavenger), becomes significantly increased after wild thyme treatment and induces the reduction of plasma lipid peroxidation in hypertensive, but not in normotensive rats. The obtained results promote wild thyme as useful supplement for cardiovascular interventions.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine & Cellular Longevity",
title = "Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress",
volume = "2016",
doi = "10.1155/2016/1458793"
}

Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Bugarski, B., Jovović, Đ., Karanović, D., Vajić, U. J., Komes, D.,& Grujić-Milanović, J.. (2016). Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress. in Oxidative Medicine & Cellular Longevity
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/1458793

Mihailović-Stanojević N, Miloradović Z, Ivanov M, Bugarski B, Jovović Đ, Karanović D, Vajić UJ, Komes D, Grujić-Milanović J. Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress. in Oxidative Medicine & Cellular Longevity. 2016;2016.
doi:10.1155/2016/1458793 .

Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Bugarski, Branko, Jovović, Đurđica, Karanović, Danijela, Vajić, Una Jovana, Komes, Draženka, Grujić-Milanović, Jelica, "Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress" in Oxidative Medicine & Cellular Longevity, 2016 (2016),
https://doi.org/10.1155/2016/1458793 . .

TY  - CHAP
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Mantzouridou, F.
AU  - Paraskevopoulou, A.
AU  - Naziri, E.
AU  - Koupantsis, T.
AU  - Trifković, Kata
AU  - Drvenica, Ivana
AU  - Balanc, Bojana
AU  - Ðordević, V.
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/607
AB  - Encapsulation is a well-established process that allows formulation of a wide range of active agents within a defined carrier material using various techniques. This chapter describes the general concept and benefits of microbial and non-microbial active food ingredient encapsulation, existing encapsulation technologies and examples of the use of loaded capsules for the protection and enhanced delivery of these constituents in food products. We describe existing knowledge of the most important characteristics of food-specific materials, capsule morphologies and methods that are applied or that may be tailored to encapsulation of active ingredients, with emphasis on microbial cells, bioactive compounds and flavours, for use in food industry. Improvements made by encapsulated cell technology in fermentation processes and in the microbial production of high-value food ingredients are reviewed. Current trends and future perspectives are also discussed.
T2  - Advances in Food Biotechnology
T1  - Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture
EP  - 680
SP  - 635
DO  - 10.1002/9781118864463.ch39
ER  - 

@inbook{
author = "Nedović, Viktor and Bugarski, Branko and Mantzouridou, F. and Paraskevopoulou, A. and Naziri, E. and Koupantsis, T. and Trifković, Kata and Drvenica, Ivana and Balanc, Bojana and Ðordević, V.",
year = "2015",
abstract = "Encapsulation is a well-established process that allows formulation of a wide range of active agents within a defined carrier material using various techniques. This chapter describes the general concept and benefits of microbial and non-microbial active food ingredient encapsulation, existing encapsulation technologies and examples of the use of loaded capsules for the protection and enhanced delivery of these constituents in food products. We describe existing knowledge of the most important characteristics of food-specific materials, capsule morphologies and methods that are applied or that may be tailored to encapsulation of active ingredients, with emphasis on microbial cells, bioactive compounds and flavours, for use in food industry. Improvements made by encapsulated cell technology in fermentation processes and in the microbial production of high-value food ingredients are reviewed. Current trends and future perspectives are also discussed.",
journal = "Advances in Food Biotechnology",
booktitle = "Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture",
pages = "680-635",
doi = "10.1002/9781118864463.ch39"
}

Nedović, V., Bugarski, B., Mantzouridou, F., Paraskevopoulou, A., Naziri, E., Koupantsis, T., Trifković, K., Drvenica, I., Balanc, B.,& Ðordević, V.. (2015). Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture. in Advances in Food Biotechnology, 635-680.
https://doi.org/10.1002/9781118864463.ch39

Nedović V, Bugarski B, Mantzouridou F, Paraskevopoulou A, Naziri E, Koupantsis T, Trifković K, Drvenica I, Balanc B, Ðordević V. Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture. in Advances in Food Biotechnology. 2015;:635-680.
doi:10.1002/9781118864463.ch39 .

Nedović, Viktor, Bugarski, Branko, Mantzouridou, F., Paraskevopoulou, A., Naziri, E., Koupantsis, T., Trifković, Kata, Drvenica, Ivana, Balanc, Bojana, Ðordević, V., "Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture" in Advances in Food Biotechnology (2015):635-680,
https://doi.org/10.1002/9781118864463.ch39 . .

TY  - JOUR
AU  - Kostić, Ivana
AU  - Ilić, Vesna
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko
AU  - Đurić, Zorka Ž.
AU  - Draškovič, Petra
AU  - Bugarski, Branko
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/655
AB  - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected.
AB  - Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
T1  - Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova
EP  - 76
IS  - 1
SP  - 67
VL  - 69
DO  - 10.2298/HEMIND140124021K
ER  - 

@article{
author = "Kostić, Ivana and Ilić, Vesna and Bukara, Katarina and Mojsilović, Slavko and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko",
year = "2015",
abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected., Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery, Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova",
pages = "76-67",
number = "1",
volume = "69",
doi = "10.2298/HEMIND140124021K"
}

Kostić, I., Ilić, V., Bukara, K., Mojsilović, S., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 69(1), 67-76.
https://doi.org/10.2298/HEMIND140124021K

Kostić I, Ilić V, Bukara K, Mojsilović S, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76.
doi:10.2298/HEMIND140124021K .

Kostić, Ivana, Ilić, Vesna, Bukara, Katarina, Mojsilović, Slavko, Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76,
https://doi.org/10.2298/HEMIND140124021K . .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Živković, Jelena
AU  - Savikin, Katarina
AU  - Gođevac, Dejan
AU  - Miloradović, Zoran
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/669
AB  - A full three level factorial design was implemented for optimization of extraction parameters in order to maximize total phenolic (TP) yield from stinging nettle leaf. Factors considered were percentage of methanol in solvent (X-1 : 50-100% methanol) and extraction time (X-2: 30-90 min), and maceration was used as extraction method. A second-order polynomial model was applied for fitting experimental data and predicting the response, and this mathematical model produced a satisfactory fit (R-2 = 0.993,p lt 0.01). The optimal extraction conditions were 54% aqueous methanol and 38 min extraction time, while maximal theoretical TP yield was 8.9 mg GAE/g DW. Solvent composition significantly affected extraction efficiency causing decrease of TP yield with increase of methanol percentage. On the other hand, extraction time did not influence significantly efficiency of extraction. Using LC/MS and HPLC analysis we detected and quantified three most abundant phenolic compounds: 2-O-caffeoyl malic acid, chlorogenic acid, and rutin. Comparison between maceration and ultrasound-assisted extraction (UAE) obtained extracts based on TP content as well as antiradical activity and HPLC results, showed that UAE have a better extraction capability affecting yield and time of extraction. Of all tested extracts, 54% aqueous methanolic extracts obtained with UAE and 38 min extraction time had the highest TP content.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops & Products
T1  - Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology
EP  - 917
SP  - 912
VL  - 74
DO  - 10.1016/j.indcrop.2015.06.032
ER  - 

@article{
author = "Vajić, Una Jovana and Grujić-Milanović, Jelica and Živković, Jelena and Savikin, Katarina and Gođevac, Dejan and Miloradović, Zoran and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2015",
abstract = "A full three level factorial design was implemented for optimization of extraction parameters in order to maximize total phenolic (TP) yield from stinging nettle leaf. Factors considered were percentage of methanol in solvent (X-1 : 50-100% methanol) and extraction time (X-2: 30-90 min), and maceration was used as extraction method. A second-order polynomial model was applied for fitting experimental data and predicting the response, and this mathematical model produced a satisfactory fit (R-2 = 0.993,p lt 0.01). The optimal extraction conditions were 54% aqueous methanol and 38 min extraction time, while maximal theoretical TP yield was 8.9 mg GAE/g DW. Solvent composition significantly affected extraction efficiency causing decrease of TP yield with increase of methanol percentage. On the other hand, extraction time did not influence significantly efficiency of extraction. Using LC/MS and HPLC analysis we detected and quantified three most abundant phenolic compounds: 2-O-caffeoyl malic acid, chlorogenic acid, and rutin. Comparison between maceration and ultrasound-assisted extraction (UAE) obtained extracts based on TP content as well as antiradical activity and HPLC results, showed that UAE have a better extraction capability affecting yield and time of extraction. Of all tested extracts, 54% aqueous methanolic extracts obtained with UAE and 38 min extraction time had the highest TP content.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops & Products",
title = "Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology",
pages = "917-912",
volume = "74",
doi = "10.1016/j.indcrop.2015.06.032"
}

Vajić, U. J., Grujić-Milanović, J., Živković, J., Savikin, K., Gođevac, D., Miloradović, Z., Bugarski, B.,& Mihailović-Stanojević, N.. (2015). Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. in Industrial Crops & Products
Elsevier, Amsterdam., 74, 912-917.
https://doi.org/10.1016/j.indcrop.2015.06.032

Vajić UJ, Grujić-Milanović J, Živković J, Savikin K, Gođevac D, Miloradović Z, Bugarski B, Mihailović-Stanojević N. Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. in Industrial Crops & Products. 2015;74:912-917.
doi:10.1016/j.indcrop.2015.06.032 .

Vajić, Una Jovana, Grujić-Milanović, Jelica, Živković, Jelena, Savikin, Katarina, Gođevac, Dejan, Miloradović, Zoran, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology" in Industrial Crops & Products, 74 (2015):912-917,
https://doi.org/10.1016/j.indcrop.2015.06.032 . .

TY  - JOUR
AU  - Dordević, Verica
AU  - Balanc, Bojana
AU  - Belscak-Cvitanović, Ana
AU  - Lević, Steva
AU  - Trifković, Kata
AU  - Kalušević, Ana
AU  - Kostić, Ivana
AU  - Komes, Draženka
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/620
AB  - The food industry expects increasingly complex properties (such as delayed release, stability, thermal protection, and suitable sensorial profile) from food ingredients, which often would not be able to be achieved without microencapsulation. This paper presents the state of the art in encapsulation technology for delivery of bioactive compounds to food. It reviews common encapsulation technologies (emphasizing their advantages and limitations) versus novel, interesting approaches in emerging technologies. This review includes a presentation of benefits resulting from the use of microencapsulated ingredients in the food industry; these benefits are going to be illustrated via few case studies bringing innovative processing. Spray drying has been used for more than 60 years to protect flavor oils against degradation/oxidation/evaporation, but melt dispersion technique has been used lately to effectively stabilize an aroma compound. Microgels produced by extrusion and emulsification techniques are considered for delivering synergistic antioxidant effects of plant extract polyphenols, their off-taste masking, and improved handling. Apart from microgels, microemulsions (produced by microfluidization or micelle formation techniques) are taken into account for entrapment of extracts containing polyphenols and essential oils. Innovative and interesting coacervation processes are depicted here as they facilitate the commercialization of coacervated food ingredients. Liposomes are gaining increasing attention in the food sector as they can provide good stability even in a water surrounding and also targeted delivery. The new scalable manufacturing protocols for the production of liposomes evolved in recent years (e.g., proliposome method) are presented here. Fluidized bed technology has been offering a versatile possibility to produce encapsulates which should release ingredients at the right place and the right time. Complex systems such as lipids in hydrogels are newly developed structures for controlled release of bioactive compounds. Finally, the effect encapsulates have when incorporated into real food products will be discussed, in particular with regard to the production of innovative functional food products. As an example, textural, sensorial, and physical quality assessment of chocolates enriched with encapsulated polyphenolic antioxidants from yarrow (Achillea millefolium L.) will be reviewed.
PB  - Springer, New York
T2  - Food Engineering Reviews
T1  - Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds
EP  - 490
IS  - 4
SP  - 452
VL  - 7
DO  - 10.1007/s12393-014-9106-7
ER  - 

@article{
author = "Dordević, Verica and Balanc, Bojana and Belscak-Cvitanović, Ana and Lević, Steva and Trifković, Kata and Kalušević, Ana and Kostić, Ivana and Komes, Draženka and Bugarski, Branko and Nedović, Viktor",
year = "2015",
abstract = "The food industry expects increasingly complex properties (such as delayed release, stability, thermal protection, and suitable sensorial profile) from food ingredients, which often would not be able to be achieved without microencapsulation. This paper presents the state of the art in encapsulation technology for delivery of bioactive compounds to food. It reviews common encapsulation technologies (emphasizing their advantages and limitations) versus novel, interesting approaches in emerging technologies. This review includes a presentation of benefits resulting from the use of microencapsulated ingredients in the food industry; these benefits are going to be illustrated via few case studies bringing innovative processing. Spray drying has been used for more than 60 years to protect flavor oils against degradation/oxidation/evaporation, but melt dispersion technique has been used lately to effectively stabilize an aroma compound. Microgels produced by extrusion and emulsification techniques are considered for delivering synergistic antioxidant effects of plant extract polyphenols, their off-taste masking, and improved handling. Apart from microgels, microemulsions (produced by microfluidization or micelle formation techniques) are taken into account for entrapment of extracts containing polyphenols and essential oils. Innovative and interesting coacervation processes are depicted here as they facilitate the commercialization of coacervated food ingredients. Liposomes are gaining increasing attention in the food sector as they can provide good stability even in a water surrounding and also targeted delivery. The new scalable manufacturing protocols for the production of liposomes evolved in recent years (e.g., proliposome method) are presented here. Fluidized bed technology has been offering a versatile possibility to produce encapsulates which should release ingredients at the right place and the right time. Complex systems such as lipids in hydrogels are newly developed structures for controlled release of bioactive compounds. Finally, the effect encapsulates have when incorporated into real food products will be discussed, in particular with regard to the production of innovative functional food products. As an example, textural, sensorial, and physical quality assessment of chocolates enriched with encapsulated polyphenolic antioxidants from yarrow (Achillea millefolium L.) will be reviewed.",
publisher = "Springer, New York",
journal = "Food Engineering Reviews",
title = "Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds",
pages = "490-452",
number = "4",
volume = "7",
doi = "10.1007/s12393-014-9106-7"
}

Dordević, V., Balanc, B., Belscak-Cvitanović, A., Lević, S., Trifković, K., Kalušević, A., Kostić, I., Komes, D., Bugarski, B.,& Nedović, V.. (2015). Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds. in Food Engineering Reviews
Springer, New York., 7(4), 452-490.
https://doi.org/10.1007/s12393-014-9106-7

Dordević V, Balanc B, Belscak-Cvitanović A, Lević S, Trifković K, Kalušević A, Kostić I, Komes D, Bugarski B, Nedović V. Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds. in Food Engineering Reviews. 2015;7(4):452-490.
doi:10.1007/s12393-014-9106-7 .

Dordević, Verica, Balanc, Bojana, Belscak-Cvitanović, Ana, Lević, Steva, Trifković, Kata, Kalušević, Ana, Kostić, Ivana, Komes, Draženka, Bugarski, Branko, Nedović, Viktor, "Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds" in Food Engineering Reviews, 7, no. 4 (2015):452-490,
https://doi.org/10.1007/s12393-014-9106-7 . .

TY  - JOUR
AU  - Kostić, Ivana
AU  - Ilić, Vesna
AU  - Đorđević Verica B.
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko
AU  - Nedović, Viktor
AU  - Bugarski, Diana
AU  - Veljović, Đorđe N.
AU  - Misić, Danijela M.
AU  - Bugarski, Branko
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/559
AB  - The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.
PB  - Elsevier, Amsterdam
T2  - Colloids & Surfaces B-Biointerfaces
T1  - Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization
EP  - 259
SP  - 250
VL  - 122
DO  - 10.1016/j.colsurfb.2014.06.043
ER  - 

@article{
author = "Kostić, Ivana and Ilić, Vesna and Đorđević Verica B. and Bukara, Katarina and Mojsilović, Slavko and Nedović, Viktor and Bugarski, Diana and Veljović, Đorđe N. and Misić, Danijela M. and Bugarski, Branko",
year = "2014",
abstract = "The present study was aimed at investigating the effect of isolation process-gradual hypotonic hemolysis on chosen parameters of the erythrocyte membranes (ghosts) originating from bovine and porcine slaughterhouse blood. The estimation of the gradual hypotonic hemolysis as a drug loading procedure for the erythrocyte ghosts was performed as well. Based on the results derived from analysis of the osmotic properties of the erythrocytes, the gradual hemolysis was performed with high volume of erythrocytes and 35 mM hypotonic sodium-phosphate/NaCl, enabling  gt 90% of hemolysis for both types of erythrocytes. Detailed insight into ghosts' morphology by field emission-scanning electron microscopy revealed a distortion from erythrocyte shape and an altered surface texture with increased bilayer curvature for both samples. Compared to erythrocytes, an average diameter of ghosts from both type of erythrocytes decreased for only about 10%. The reported unidispersity of the isolated ghosts is of great importance for their potential application as vehicles of active compounds. Gradual hemolysis did not lead to substantial loss of cholesterol and membrane/cytoskeleton proteins. This result indicated the ghosts' possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, which is of significance for drug diffusion and partition coefficients. Induced shift of phosphatidylserine to external surface of the ghosts demonstrated their potential application as vehicles for targeted drug delivery to cells of reticuloendothelial system. Ultra high-performance liquid chromatography and Fourier transform infrared spectroscopy revealed the presence of a drug model - dexamethasone-sodium phosphate, and its interaction with structural components in both types of erythrocyte ghosts.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids & Surfaces B-Biointerfaces",
title = "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization",
pages = "259-250",
volume = "122",
doi = "10.1016/j.colsurfb.2014.06.043"
}

Kostić, I., Ilić, V., Đorđević Verica B., Bukara, K., Mojsilović, S., Nedović, V., Bugarski, D., Veljović, Đ. N., Misić, D. M.,& Bugarski, B.. (2014). Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids & Surfaces B-Biointerfaces
Elsevier, Amsterdam., 122, 250-259.
https://doi.org/10.1016/j.colsurfb.2014.06.043

Kostić I, Ilić V, Đorđević Verica B., Bukara K, Mojsilović S, Nedović V, Bugarski D, Veljović ĐN, Misić DM, Bugarski B. Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization. in Colloids & Surfaces B-Biointerfaces. 2014;122:250-259.
doi:10.1016/j.colsurfb.2014.06.043 .

Kostić, Ivana, Ilić, Vesna, Đorđević Verica B., Bukara, Katarina, Mojsilović, Slavko, Nedović, Viktor, Bugarski, Diana, Veljović, Đorđe N., Misić, Danijela M., Bugarski, Branko, "Erythrocyte membranes from slaughterhouse blood as potential drug vehicles: Isolation by gradual hypotonic hemolysis and biochemical and morphological characterization" in Colloids & Surfaces B-Biointerfaces, 122 (2014):250-259,
https://doi.org/10.1016/j.colsurfb.2014.06.043 . .