TY  - JOUR
AU  - Stojanović, Radoslava
AU  - Ilić, Vesna
AU  - Manojlović, Verica
AU  - Bugarski, Diana
AU  - Dević, Marija
AU  - Bugarski, Branko
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/431
AB  - In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system that provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts) was designed at a pilot scale. Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling, and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 88% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The hemoglobin purity of  gt 80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min. The dynamics of both steps, (1) transport through the membrane of erythrocytes during process of hemolysis and (2) transport through the reactor filters, were evaluated.
PB  - Springer, New York
T2  - Applied Biochemistry & Biotechnology
T1  - Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor
EP  - 1506
IS  - 6
SP  - 1491
VL  - 166
DO  - 10.1007/s12010-012-9543-9
ER  - 

@article{
author = "Stojanović, Radoslava and Ilić, Vesna and Manojlović, Verica and Bugarski, Diana and Dević, Marija and Bugarski, Branko",
year = "2012",
abstract = "In this work, we describe an optimized procedure based on gradual hemolysis for the isolation of hemoglobin derived from bovine slaughterhouse erythrocytes in a membrane bioreactor. The membrane bioreactor system that provided high yields of hemoglobin (mainly oxyhemoglobin derivate) and its separation from the empty erythrocyte membranes (ghosts) was designed at a pilot scale. Ten different concentrations of hypotonic media were assessed from the aspect of the extent of hemolysis, hematocrit values of the erythrocyte suspensions, cell swelling, and membrane deformations induced by decreased salt concentration. Effective gradual osmotic hemolysis with an extent of hemolysis of 88% was performed using 35 mM Na-phosphate/NaCl buffer of pH 7.2-7.4. Under these conditions most of the cell membranes presented the appearance of the normal ghosts under phase contrast microscope. The hemoglobin purity of  gt 80% was confirmed by SDS-PAGE. Kinetic studies showed that maximal concentration of hemoglobin was reached after 40 min, but the process cycle at which recovery of 83% was achieved lasted for 90 min. The dynamics of both steps, (1) transport through the membrane of erythrocytes during process of hemolysis and (2) transport through the reactor filters, were evaluated.",
publisher = "Springer, New York",
journal = "Applied Biochemistry & Biotechnology",
title = "Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor",
pages = "1506-1491",
number = "6",
volume = "166",
doi = "10.1007/s12010-012-9543-9"
}

Stojanović, R., Ilić, V., Manojlović, V., Bugarski, D., Dević, M.,& Bugarski, B.. (2012). Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor. in Applied Biochemistry & Biotechnology
Springer, New York., 166(6), 1491-1506.
https://doi.org/10.1007/s12010-012-9543-9

Stojanović R, Ilić V, Manojlović V, Bugarski D, Dević M, Bugarski B. Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor. in Applied Biochemistry & Biotechnology. 2012;166(6):1491-1506.
doi:10.1007/s12010-012-9543-9 .

Stojanović, Radoslava, Ilić, Vesna, Manojlović, Verica, Bugarski, Diana, Dević, Marija, Bugarski, Branko, "Isolation of Hemoglobin from Bovine Erythrocytes by Controlled Hemolysis in the Membrane Bioreactor" in Applied Biochemistry & Biotechnology, 166, no. 6 (2012):1491-1506,
https://doi.org/10.1007/s12010-012-9543-9 . .