TY  - JOUR
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Bogosavljević, Nikola
AU  - Jurišić, Vladimir
AU  - Jauković, Aleksandra
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1460
AB  - Among multiple hemostasis components, platelets hyperactivity plays major roles in cancer progression by providing surface and internal components for intercellular crosstalk as well as by behaving like immune cells. Since platelets participate and regulate immunity in homeostatic and disease states, we assumed that revealing platelets profile might help in conceiving novel anti-cancer immune-based strategies. The goal of this review is to compile and discuss the most recent reports on the nature of cancer-associated platelets and their interference with immunotherapy. An increasing number of studies have emphasized active communication between cancer cells and platelets, with platelets promoting cancer cell survival, growth, and metastasis. The anti-cancer potential of platelet-directed therapy has been intensively investigated, and anti-platelet agents may prevent cancer progression and improve the survival of cancer patients. Platelets can (i) reduce antitumor activity; (ii) support immunoregulatory cells and factors generation; (iii) underpin metastasis and, (iv) interfere with immunotherapy by expressing ligands of immune checkpoint receptors. Mediators produced by tumor cell-induced platelet activation support vein thrombosis, constrain anti-tumor T- and natural killer cell response, while contributing to extravasation of tumor cells, metastatic potential, and neovascularization within the tumor. Recent studies showed that attenuation of immunothrombosis, modulation of platelets and their factors have a good perspective in immunotherapy optimization. Particularly, blockade of intra-tumoral platelet-associated programmed death-ligand 1 might promote anti-tumor T cell-induced cytotoxicity. Collectively, these findings suggest that platelets might represent the source of relevant cancer staging biomarkers, as well as promising targets and carriers in immunotherapeutic approaches for combating cancer.
PB  - Neoplasia Press, Inc.
T2  - Translational Oncology
T2  - Translational OncologyTranslational Oncology
T1  - Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development
SP  - 101871
VL  - 40
DO  - 10.1016/j.tranon.2023.101871
ER  - 

@article{
author = "Trivanović, Drenka and Mojsilović, Slavko and Bogosavljević, Nikola and Jurišić, Vladimir and Jauković, Aleksandra",
year = "2024",
abstract = "Among multiple hemostasis components, platelets hyperactivity plays major roles in cancer progression by providing surface and internal components for intercellular crosstalk as well as by behaving like immune cells. Since platelets participate and regulate immunity in homeostatic and disease states, we assumed that revealing platelets profile might help in conceiving novel anti-cancer immune-based strategies. The goal of this review is to compile and discuss the most recent reports on the nature of cancer-associated platelets and their interference with immunotherapy. An increasing number of studies have emphasized active communication between cancer cells and platelets, with platelets promoting cancer cell survival, growth, and metastasis. The anti-cancer potential of platelet-directed therapy has been intensively investigated, and anti-platelet agents may prevent cancer progression and improve the survival of cancer patients. Platelets can (i) reduce antitumor activity; (ii) support immunoregulatory cells and factors generation; (iii) underpin metastasis and, (iv) interfere with immunotherapy by expressing ligands of immune checkpoint receptors. Mediators produced by tumor cell-induced platelet activation support vein thrombosis, constrain anti-tumor T- and natural killer cell response, while contributing to extravasation of tumor cells, metastatic potential, and neovascularization within the tumor. Recent studies showed that attenuation of immunothrombosis, modulation of platelets and their factors have a good perspective in immunotherapy optimization. Particularly, blockade of intra-tumoral platelet-associated programmed death-ligand 1 might promote anti-tumor T cell-induced cytotoxicity. Collectively, these findings suggest that platelets might represent the source of relevant cancer staging biomarkers, as well as promising targets and carriers in immunotherapeutic approaches for combating cancer.",
publisher = "Neoplasia Press, Inc.",
journal = "Translational Oncology, Translational OncologyTranslational Oncology",
title = "Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development",
pages = "101871",
volume = "40",
doi = "10.1016/j.tranon.2023.101871"
}

Trivanović, D., Mojsilović, S., Bogosavljević, N., Jurišić, V.,& Jauković, A.. (2024). Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development. in Translational Oncology
Neoplasia Press, Inc.., 40, 101871.
https://doi.org/10.1016/j.tranon.2023.101871

Trivanović D, Mojsilović S, Bogosavljević N, Jurišić V, Jauković A. Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development. in Translational Oncology. 2024;40:101871.
doi:10.1016/j.tranon.2023.101871 .

Trivanović, Drenka, Mojsilović, Slavko, Bogosavljević, Nikola, Jurišić, Vladimir, Jauković, Aleksandra, "Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development" in Translational Oncology, 40 (2024):101871,
https://doi.org/10.1016/j.tranon.2023.101871 . .

TY  - CONF
AU  - Ristić, Biljana
AU  - Trpkov, Đorđe
AU  - Drvenica, Ivana
AU  - Dojčilović, Radovan
AU  - Đukić, Tamara
AU  - Tošić, Dragana
AU  - Pajović, Jelena
AU  - Božanić, Dušan K.
AU  - Trivanović, Drenka
AU  - Matić, Tamara
AU  - Sredojević, Dušan
AU  - Ilić, Vesna
AU  - Đoković, Vladimir
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1471
AB  - Nitrogen-doped carbon quantum dots (N-CQDs) are promising next
generation nanomaterials for potential biomedical applications such as bioimaging, biosensing,
and drug/gene delivery. However, N-CQDs biocompatibility has not been extensively
investigated. Here, we report physico-chemical characteristics of newly synthetized N-CQDs
and their effects on red blood cells (RBC), by analyzing their hemolytic activity, impact on
RBC rheology/morphology, and oxidative stress induction. N-CQDs were prepared by
hydrothermal method using citric acid and urea as precursors. Structural analyses of as prepared
N-GQDs, observed by HRTEM/EDS, showed that the lateral dimensions of the particles are in
the 10 to 20 nm range, as well as that the carbon, oxygen, and nitrogen are present in the
nanosystem. Based on AFM measurements, the average height of N-CQDs was 3.9±0.08 Å.
Photoluminescence emission (PLE) spectrum demonstrated that N-CQDs exhibit stable and
strong fluorescence in green (520 nm) region, upon 410 nm excitation. FTIR spectroscopy
indicated vibrational bands, characteristic for carbon structures and primary amines (Ndoping).
N-CQDs were negatively charged with an average Zeta potential of -15.3
mV as confirmed by DLS. To investigate hemocompatibility of N-CQDs, the RBC, the most
abundant cells in blood, were treated with different concentration of N-CQDs (10-400 ug/ml)
for 2h. Obtained results showed that there was no hemolytic activity. Moreover, ektacytometry
analysis demonstrated that N-CQDs did not affect deformability of RBC. Fluorescent
microscopy analyses revealed that treatment with N-CQDs did not induce significant
morphological aberrant forms of RBC which was also confirmed by SEM analyses. Flow
cytometry confirmed only slight RBC morphological changes based on FSC/SSC analysis.
Furthermore, using ROS sensitive dye flow cytometry analyses suggested that N-CQDs did not
induce oxidative stress in RBC. Taken together, our findings highlighted that exposure of RBC
to N-CQDs only led to the attachment of N-CQDs on RBC membranes, but there is no other
evidence of their nanotoxicity. These findings suggested that N-CQDs synthetized from ecofriendly
precursors are potentially biocompatible and safe for biomedical application.
PB  - Belgrade : Institute of Physics
C3  - Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
T1  - Hemocompatibility evaluation of N-doped carbon quantum dots
EP  - 47
SP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1471
ER  - 

@conference{
author = "Ristić, Biljana and Trpkov, Đorđe and Drvenica, Ivana and Dojčilović, Radovan and Đukić, Tamara and Tošić, Dragana and Pajović, Jelena and Božanić, Dušan K. and Trivanović, Drenka and Matić, Tamara and Sredojević, Dušan and Ilić, Vesna and Đoković, Vladimir",
year = "2024",
abstract = "Nitrogen-doped carbon quantum dots (N-CQDs) are promising next
generation nanomaterials for potential biomedical applications such as bioimaging, biosensing,
and drug/gene delivery. However, N-CQDs biocompatibility has not been extensively
investigated. Here, we report physico-chemical characteristics of newly synthetized N-CQDs
and their effects on red blood cells (RBC), by analyzing their hemolytic activity, impact on
RBC rheology/morphology, and oxidative stress induction. N-CQDs were prepared by
hydrothermal method using citric acid and urea as precursors. Structural analyses of as prepared
N-GQDs, observed by HRTEM/EDS, showed that the lateral dimensions of the particles are in
the 10 to 20 nm range, as well as that the carbon, oxygen, and nitrogen are present in the
nanosystem. Based on AFM measurements, the average height of N-CQDs was 3.9±0.08 Å.
Photoluminescence emission (PLE) spectrum demonstrated that N-CQDs exhibit stable and
strong fluorescence in green (520 nm) region, upon 410 nm excitation. FTIR spectroscopy
indicated vibrational bands, characteristic for carbon structures and primary amines (Ndoping).
N-CQDs were negatively charged with an average Zeta potential of -15.3
mV as confirmed by DLS. To investigate hemocompatibility of N-CQDs, the RBC, the most
abundant cells in blood, were treated with different concentration of N-CQDs (10-400 ug/ml)
for 2h. Obtained results showed that there was no hemolytic activity. Moreover, ektacytometry
analysis demonstrated that N-CQDs did not affect deformability of RBC. Fluorescent
microscopy analyses revealed that treatment with N-CQDs did not induce significant
morphological aberrant forms of RBC which was also confirmed by SEM analyses. Flow
cytometry confirmed only slight RBC morphological changes based on FSC/SSC analysis.
Furthermore, using ROS sensitive dye flow cytometry analyses suggested that N-CQDs did not
induce oxidative stress in RBC. Taken together, our findings highlighted that exposure of RBC
to N-CQDs only led to the attachment of N-CQDs on RBC membranes, but there is no other
evidence of their nanotoxicity. These findings suggested that N-CQDs synthetized from ecofriendly
precursors are potentially biocompatible and safe for biomedical application.",
publisher = "Belgrade : Institute of Physics",
journal = "Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024",
title = "Hemocompatibility evaluation of N-doped carbon quantum dots",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1471"
}

Ristić, B., Trpkov, Đ., Drvenica, I., Dojčilović, R., Đukić, T., Tošić, D., Pajović, J., Božanić, D. K., Trivanović, D., Matić, T., Sredojević, D., Ilić, V.,& Đoković, V.. (2024). Hemocompatibility evaluation of N-doped carbon quantum dots. in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024
Belgrade : Institute of Physics., 47-47.
https://hdl.handle.net/21.15107/rcub_rimi_1471

Ristić B, Trpkov Đ, Drvenica I, Dojčilović R, Đukić T, Tošić D, Pajović J, Božanić DK, Trivanović D, Matić T, Sredojević D, Ilić V, Đoković V. Hemocompatibility evaluation of N-doped carbon quantum dots. in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024. 2024;:47-47.
https://hdl.handle.net/21.15107/rcub_rimi_1471 .

Ristić, Biljana, Trpkov, Đorđe, Drvenica, Ivana, Dojčilović, Radovan, Đukić, Tamara, Tošić, Dragana, Pajović, Jelena, Božanić, Dušan K., Trivanović, Drenka, Matić, Tamara, Sredojević, Dušan, Ilić, Vesna, Đoković, Vladimir, "Hemocompatibility evaluation of N-doped carbon quantum dots" in Book of Abstracts: 17th Photonics Workshop, (Conference), Kopaonik, March 10-14, 2024 (2024):47-47,
https://hdl.handle.net/21.15107/rcub_rimi_1471 .

TY  - JOUR
AU  - Amorim, Tânia
AU  - Trivanović, Drenka
AU  - Benova, Andrea
AU  - Li, Hongshuai
AU  - Tencerova, Michaela
AU  - Palmisano, Biagio
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1472
AB  - Bone marrow adiposity (BMA) is a rapidly growing yet very young research field that is receiving worldwide attention based on its intimate relationship with skeletal and metabolic diseases, as well as hematology and cancer. Moreover, increasing numbers of young scientists and students are currently and actively working on BMA within their research projects. These developments led to the foundation of the International Bone Marrow Adiposity Society (BMAS), with the goal to promote BMA knowledge worldwide, and to train new generations of researchers interested in studying this field. Among the many initiatives supported by BMAS, there is the BMAS Summer School, inaugurated in 2021 and now at its second edition. The aim of the BMAS Summer School 2023 was to educate and train students by disseminating the latest advancement on BMA. Moreover, Summer School 2023 provided suggestions on how to write grants, deal with negative results in science, and start a laboratory, along with illustrations of alternative paths to academia. The event was animated by constructive and interactive discussions between early-career researchers and more senior scientists. In this report, we highlight key moments and lessons learned from the event.
PB  - Company of Biologists Ltd.
T2  - Biology Open
T1  - Young minds, deeper insights: a recap of the BMAS Summer School 2023, ranging from basic research to clinical implications of bone marrow adipose tissue
IS  - 2
SP  - bio060263
VL  - 13
DO  - 10.1242/bio.060263
ER  - 

@article{
author = "Amorim, Tânia and Trivanović, Drenka and Benova, Andrea and Li, Hongshuai and Tencerova, Michaela and Palmisano, Biagio",
year = "2024",
abstract = "Bone marrow adiposity (BMA) is a rapidly growing yet very young research field that is receiving worldwide attention based on its intimate relationship with skeletal and metabolic diseases, as well as hematology and cancer. Moreover, increasing numbers of young scientists and students are currently and actively working on BMA within their research projects. These developments led to the foundation of the International Bone Marrow Adiposity Society (BMAS), with the goal to promote BMA knowledge worldwide, and to train new generations of researchers interested in studying this field. Among the many initiatives supported by BMAS, there is the BMAS Summer School, inaugurated in 2021 and now at its second edition. The aim of the BMAS Summer School 2023 was to educate and train students by disseminating the latest advancement on BMA. Moreover, Summer School 2023 provided suggestions on how to write grants, deal with negative results in science, and start a laboratory, along with illustrations of alternative paths to academia. The event was animated by constructive and interactive discussions between early-career researchers and more senior scientists. In this report, we highlight key moments and lessons learned from the event.",
publisher = "Company of Biologists Ltd.",
journal = "Biology Open",
title = "Young minds, deeper insights: a recap of the BMAS Summer School 2023, ranging from basic research to clinical implications of bone marrow adipose tissue",
number = "2",
pages = "bio060263",
volume = "13",
doi = "10.1242/bio.060263"
}

Amorim, T., Trivanović, D., Benova, A., Li, H., Tencerova, M.,& Palmisano, B.. (2024). Young minds, deeper insights: a recap of the BMAS Summer School 2023, ranging from basic research to clinical implications of bone marrow adipose tissue. in Biology Open
Company of Biologists Ltd.., 13(2), bio060263.
https://doi.org/10.1242/bio.060263

Amorim T, Trivanović D, Benova A, Li H, Tencerova M, Palmisano B. Young minds, deeper insights: a recap of the BMAS Summer School 2023, ranging from basic research to clinical implications of bone marrow adipose tissue. in Biology Open. 2024;13(2):bio060263.
doi:10.1242/bio.060263 .

Amorim, Tânia, Trivanović, Drenka, Benova, Andrea, Li, Hongshuai, Tencerova, Michaela, Palmisano, Biagio, "Young minds, deeper insights: a recap of the BMAS Summer School 2023, ranging from basic research to clinical implications of bone marrow adipose tissue" in Biology Open, 13, no. 2 (2024):bio060263,
https://doi.org/10.1242/bio.060263 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1346
AB  - Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T2  - Biomolecules
T1  - The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis
IS  - 10
SP  - 1437
VL  - 13
DO  - 10.3390/biom13101437
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules, Biomolecules",
title = "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis",
number = "10",
pages = "1437",
volume = "13",
doi = "10.3390/biom13101437"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 13(10), 1437.
https://doi.org/10.3390/biom13101437

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules. 2023;13(10):1437.
doi:10.3390/biom13101437 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis" in Biomolecules, 13, no. 10 (2023):1437,
https://doi.org/10.3390/biom13101437 . .

TY  - CONF
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1406
AB  - Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.
PB  - Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije
C3  - Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
T1  - Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1406
ER  - 

@conference{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.",
publisher = "Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije",
journal = "Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka",
title = "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1406"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije..
https://hdl.handle.net/21.15107/rcub_rimi_1406

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka. 2023;.
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma" in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka (2023),
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Vujačić, Marko
AU  - Arsić, Aleksandra
AU  - Bogosavljević, Nikola
AU  - Kovačić, Marijana
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Baščarević, Zoran
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1408
AB  - Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients
IS  - 3
SP  - P140
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1408
ER  - 

@conference{
author = "Trivanović, Drenka and Vujačić, Marko and Arsić, Aleksandra and Bogosavljević, Nikola and Kovačić, Marijana and Drvenica, Ivana and Mojsilović, Slavko and Baščarević, Zoran and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients",
number = "3",
pages = "P140",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1408"
}

Trivanović, D., Vujačić, M., Arsić, A., Bogosavljević, N., Kovačić, M., Drvenica, I., Mojsilović, S., Baščarević, Z., Bugarski, D.,& Jauković, A.. (2023). Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408

Trivanović D, Vujačić M, Arsić A, Bogosavljević N, Kovačić M, Drvenica I, Mojsilović S, Baščarević Z, Bugarski D, Jauković A. Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

Trivanović, Drenka, Vujačić, Marko, Arsić, Aleksandra, Bogosavljević, Nikola, Kovačić, Marijana, Drvenica, Ivana, Mojsilović, Slavko, Baščarević, Zoran, Bugarski, Diana, Jauković, Aleksandra, "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P140,
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Labella, Rossella
AU  - Tratwal, Josefine
AU  - Bugarski, Diana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1268
PB  - Frontiers Media S.A.
T2  - Frontiers in Cell and Developmental Biology
T1  - Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease
SP  - 1095235
VL  - 10
DO  - 10.3389/fcell.2022.1095235
ER  - 

@article{
author = "Trivanović, Drenka and Labella, Rossella and Tratwal, Josefine and Bugarski, Diana",
year = "2023",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cell and Developmental Biology",
title = "Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease",
pages = "1095235",
volume = "10",
doi = "10.3389/fcell.2022.1095235"
}

Trivanović, D., Labella, R., Tratwal, J.,& Bugarski, D.. (2023). Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease. in Frontiers in Cell and Developmental Biology
Frontiers Media S.A.., 10, 1095235.
https://doi.org/10.3389/fcell.2022.1095235

Trivanović D, Labella R, Tratwal J, Bugarski D. Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease. in Frontiers in Cell and Developmental Biology. 2023;10:1095235.
doi:10.3389/fcell.2022.1095235 .

Trivanović, Drenka, Labella, Rossella, Tratwal, Josefine, Bugarski, Diana, "Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease" in Frontiers in Cell and Developmental Biology, 10 (2023):1095235,
https://doi.org/10.3389/fcell.2022.1095235 . .

TY  - JOUR
AU  - Labella, Rossella
AU  - Vujačić, Marko
AU  - Trivanović, Drenka
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1394
AB  - Bone marrow adipose tissue (BMAT) creates a specific microniche within multifunctional bone marrow (BM) ecosystem which imposes changes in surrounding cells and at systemic level. Moreover, BMAT contributes to spatial and temporal separation and metabolic compartmentalization of BM, thus regulating BM homeostasis and diseases. Recent findings have identified novel progenitor subsets of bone marrow adipocytes (BMAd)s recruited during the BM adipogenesis within different skeletal and hematopoietic stem cell niches. Potential of certain mesenchymal BM cells to differentiate into both osteogenic and adipogenic lineages, contributes to the complex interplay of BMAT with endosteal (osteoblastic) niche compartments as an important cellular player in bone tissue homeostasis. Targeting and ablation of BMAT cells at certain states might be an optional and promising strategy for improvement of bone health. Additionally, recent findings demonstrated spatial distribution of BMAds related to hematopoietic cells and pointed out important functional roles in the vital processes such as long-term hematopoiesis. BM adipogenesis appears to be an emergency phenomenon that follows the production of hematopoietic stem and progenitor cell niche factors, thus regulating physiological, stressed, and malignant hematopoiesis. Lipolytic and secretory activity of BMAds can influence survival and proliferation of hematopoietic cells at different maturation stages. Due to their different lipid status, constitutive and regulated BMAds are important determinants of normal and malignant hematopoietic cells. Further elucidation of cellular and molecular players involved in BMAT expansion and crosstalk with malignant cells is of paramount importance for conceiving the new therapies for improvement of BM health.
PB  - Springer Nature
T2  - Stem Cell Reviews and Reports
T1  - Bone Marrow Adipose Tissue: Regulation of Osteoblastic Niche, Hematopoiesis and Hematological Malignancies
EP  - 1151
IS  - 5
SP  - 1135
VL  - 19
DO  - 10.1007/s12015-023-10531-3
ER  - 

@article{
author = "Labella, Rossella and Vujačić, Marko and Trivanović, Drenka",
year = "2023",
abstract = "Bone marrow adipose tissue (BMAT) creates a specific microniche within multifunctional bone marrow (BM) ecosystem which imposes changes in surrounding cells and at systemic level. Moreover, BMAT contributes to spatial and temporal separation and metabolic compartmentalization of BM, thus regulating BM homeostasis and diseases. Recent findings have identified novel progenitor subsets of bone marrow adipocytes (BMAd)s recruited during the BM adipogenesis within different skeletal and hematopoietic stem cell niches. Potential of certain mesenchymal BM cells to differentiate into both osteogenic and adipogenic lineages, contributes to the complex interplay of BMAT with endosteal (osteoblastic) niche compartments as an important cellular player in bone tissue homeostasis. Targeting and ablation of BMAT cells at certain states might be an optional and promising strategy for improvement of bone health. Additionally, recent findings demonstrated spatial distribution of BMAds related to hematopoietic cells and pointed out important functional roles in the vital processes such as long-term hematopoiesis. BM adipogenesis appears to be an emergency phenomenon that follows the production of hematopoietic stem and progenitor cell niche factors, thus regulating physiological, stressed, and malignant hematopoiesis. Lipolytic and secretory activity of BMAds can influence survival and proliferation of hematopoietic cells at different maturation stages. Due to their different lipid status, constitutive and regulated BMAds are important determinants of normal and malignant hematopoietic cells. Further elucidation of cellular and molecular players involved in BMAT expansion and crosstalk with malignant cells is of paramount importance for conceiving the new therapies for improvement of BM health.",
publisher = "Springer Nature",
journal = "Stem Cell Reviews and Reports",
title = "Bone Marrow Adipose Tissue: Regulation of Osteoblastic Niche, Hematopoiesis and Hematological Malignancies",
pages = "1151-1135",
number = "5",
volume = "19",
doi = "10.1007/s12015-023-10531-3"
}

Labella, R., Vujačić, M.,& Trivanović, D.. (2023). Bone Marrow Adipose Tissue: Regulation of Osteoblastic Niche, Hematopoiesis and Hematological Malignancies. in Stem Cell Reviews and Reports
Springer Nature., 19(5), 1135-1151.
https://doi.org/10.1007/s12015-023-10531-3

Labella R, Vujačić M, Trivanović D. Bone Marrow Adipose Tissue: Regulation of Osteoblastic Niche, Hematopoiesis and Hematological Malignancies. in Stem Cell Reviews and Reports. 2023;19(5):1135-1151.
doi:10.1007/s12015-023-10531-3 .

Labella, Rossella, Vujačić, Marko, Trivanović, Drenka, "Bone Marrow Adipose Tissue: Regulation of Osteoblastic Niche, Hematopoiesis and Hematological Malignancies" in Stem Cell Reviews and Reports, 19, no. 5 (2023):1135-1151,
https://doi.org/10.1007/s12015-023-10531-3 . .

TY  - CONF
AU  - Drvenica, Ivana
AU  - Trivanović, Drenka
AU  - Radmilović, Mihajlo
AU  - Vučetić, Dušan
AU  - Krmpot, Aleksandar
AU  - Ilić, Vesna
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1405
AB  - Ektacytometry, a diffraction-based method, measures the deformability of entire erythrocyte populations and does not provide information on the altered deformability of individual erythrocytes or affected subpopulation induced by constant oxidative stress, physical stress, metabolic depletion, and loss of ion gradients during their 120 days life span. We introduced an approach based on ektacytometry coupled to flow cytometry analysis to monitor the deformability and heterogeneity of subpopulations of erythrocytes. The effects of in vitro changes of osmotic gradient (from 155 mM to 93 mM phosphate buffer) and treatment by oxidative agent (0.5 mM and 0.75 mM terc-bytil hydroperoxide (TBPH)) on human erythrocyte isolated from healthy male donors, were tested using RheoScan D 300 (RheoMeditech. Inc., Korea) and BD FACSCalibur flow cytometer (Becton Dickinson, USA).
A decrease in erythrocyte deformability by changes in osmolality or treatment with TBPH 
per se was demonstrated by ektacytometry. Nevertheless, this method could not analyze the 
erythrocytes that underwent both treatments due to their lysis by the shear stress in the 
device. The samples of an equal population of normal erythrocytes and erythrocytes 
rigidified by 0.5 mM TBPH (slightly rigid) showed elongation indices in the physiological 
range, i.e., the effect of the treatments was annulled. The same result was obtained by flow 
cytometry. On the other hand, an altered population of oxidized cells by 0.5 mM TBPH was 
detected in hypoosmotic 93 mM buffer based on their forward scatter (FSC) (Figure 1) and 
side scatter (SSC) parameters. The subtle changes in the erythrocyte’s subpopulation 
mechanobiology are essential to monitoring even in healthy people exposed to physical 
or/and environmental stress and stored erythrocytes, but some additional techniques are 
needed for the established optical-based approaches.
PB  - Belgrade: Institute of Physics
C3  - 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts
T1  - Optical methodologies in the analysis of erythrocyte deformability and heterogeneity
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1405
ER  - 

@conference{
author = "Drvenica, Ivana and Trivanović, Drenka and Radmilović, Mihajlo and Vučetić, Dušan and Krmpot, Aleksandar and Ilić, Vesna",
year = "2023",
abstract = "Ektacytometry, a diffraction-based method, measures the deformability of entire erythrocyte populations and does not provide information on the altered deformability of individual erythrocytes or affected subpopulation induced by constant oxidative stress, physical stress, metabolic depletion, and loss of ion gradients during their 120 days life span. We introduced an approach based on ektacytometry coupled to flow cytometry analysis to monitor the deformability and heterogeneity of subpopulations of erythrocytes. The effects of in vitro changes of osmotic gradient (from 155 mM to 93 mM phosphate buffer) and treatment by oxidative agent (0.5 mM and 0.75 mM terc-bytil hydroperoxide (TBPH)) on human erythrocyte isolated from healthy male donors, were tested using RheoScan D 300 (RheoMeditech. Inc., Korea) and BD FACSCalibur flow cytometer (Becton Dickinson, USA).
A decrease in erythrocyte deformability by changes in osmolality or treatment with TBPH 
per se was demonstrated by ektacytometry. Nevertheless, this method could not analyze the 
erythrocytes that underwent both treatments due to their lysis by the shear stress in the 
device. The samples of an equal population of normal erythrocytes and erythrocytes 
rigidified by 0.5 mM TBPH (slightly rigid) showed elongation indices in the physiological 
range, i.e., the effect of the treatments was annulled. The same result was obtained by flow 
cytometry. On the other hand, an altered population of oxidized cells by 0.5 mM TBPH was 
detected in hypoosmotic 93 mM buffer based on their forward scatter (FSC) (Figure 1) and 
side scatter (SSC) parameters. The subtle changes in the erythrocyte’s subpopulation 
mechanobiology are essential to monitoring even in healthy people exposed to physical 
or/and environmental stress and stored erythrocytes, but some additional techniques are 
needed for the established optical-based approaches.",
publisher = "Belgrade: Institute of Physics",
journal = "16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts",
title = "Optical methodologies in the analysis of erythrocyte deformability and heterogeneity",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1405"
}

Drvenica, I., Trivanović, D., Radmilović, M., Vučetić, D., Krmpot, A.,& Ilić, V.. (2023). Optical methodologies in the analysis of erythrocyte deformability and heterogeneity. in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts
Belgrade: Institute of Physics., 27-27.
https://hdl.handle.net/21.15107/rcub_rimi_1405

Drvenica I, Trivanović D, Radmilović M, Vučetić D, Krmpot A, Ilić V. Optical methodologies in the analysis of erythrocyte deformability and heterogeneity. in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts. 2023;:27-27.
https://hdl.handle.net/21.15107/rcub_rimi_1405 .

Drvenica, Ivana, Trivanović, Drenka, Radmilović, Mihajlo, Vučetić, Dušan, Krmpot, Aleksandar, Ilić, Vesna, "Optical methodologies in the analysis of erythrocyte deformability and heterogeneity" in 16th Photonics Workshop, (Conference) Kopaonik, March 12-15, 2023 - Book of Abstracts (2023):27-27,
https://hdl.handle.net/21.15107/rcub_rimi_1405 .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Živanović, Milena
AU  - Vujačić, Marko
AU  - Bogosavljević, Nikola
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1409
AB  - Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation
IS  - 3
SP  - P141
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1409
ER  - 

@conference{
author = "Trivanović, Drenka and Okić Đorđević, Ivana and Živanović, Milena and Vujačić, Marko and Bogosavljević, Nikola and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation",
number = "3",
pages = "P141",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1409"
}

Trivanović, D., Okić Đorđević, I., Živanović, M., Vujačić, M., Bogosavljević, N., Bugarski, D.,& Jauković, A.. (2023). Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409

Trivanović D, Okić Đorđević I, Živanović M, Vujačić M, Bogosavljević N, Bugarski D, Jauković A. Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

Trivanović, Drenka, Okić Đorđević, Ivana, Živanović, Milena, Vujačić, Marko, Bogosavljević, Nikola, Bugarski, Diana, Jauković, Aleksandra, "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P141,
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Miletić, Maja
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1184
AB  - Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
PB  - Srpsko biološko društvo
T2  - Archives of Biological Sciences
T1  - Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
EP  - 24
IS  - 1
SP  - 15
VL  - 74
DO  - 10.2298/ABS210929048O
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Trivanović, Drenka and Petrović, Anđelija and Mojsilović, Slavko and Miletić, Maja and Bugarski, Diana and Jauković, Aleksandra",
year = "2022",
abstract = "Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.",
publisher = "Srpsko biološko društvo",
journal = "Archives of Biological Sciences",
title = "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway",
pages = "24-15",
number = "1",
volume = "74",
doi = "10.2298/ABS210929048O"
}

Okić Đorđević, I., Kukolj, T., Obradović, H., Trivanović, D., Petrović, A., Mojsilović, S., Miletić, M., Bugarski, D.,& Jauković, A.. (2022). Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences
Srpsko biološko društvo., 74(1), 15-24.
https://doi.org/10.2298/ABS210929048O

Okić Đorđević I, Kukolj T, Obradović H, Trivanović D, Petrović A, Mojsilović S, Miletić M, Bugarski D, Jauković A. Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences. 2022;74(1):15-24.
doi:10.2298/ABS210929048O .

Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Trivanović, Drenka, Petrović, Anđelija, Mojsilović, Slavko, Miletić, Maja, Bugarski, Diana, Jauković, Aleksandra, "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway" in Archives of Biological Sciences, 74, no. 1 (2022):15-24,
https://doi.org/10.2298/ABS210929048O . .

TY  - JOUR
AU  - Borojević, Ana
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Mojsilović, Slavko
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Živanović, Milena
AU  - Zečević, Željko
AU  - Simić, Marija
AU  - Gobeljić, Borko
AU  - Vujić, Dragana
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1207
AB  - The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T1  - Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
IS  - 2
SP  - 323
VL  - 12
DO  - 10.3390/biom12020323
ER  - 

@article{
author = "Borojević, Ana and Jauković, Aleksandra and Kukolj, Tamara and Mojsilović, Slavko and Obradović, Hristina and Trivanović, Drenka and Živanović, Milena and Zečević, Željko and Simić, Marija and Gobeljić, Borko and Vujić, Dragana and Bugarski, Diana",
year = "2022",
abstract = "The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules",
title = "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling",
number = "2",
pages = "323",
volume = "12",
doi = "10.3390/biom12020323"
}

Borojević, A., Jauković, A., Kukolj, T., Mojsilović, S., Obradović, H., Trivanović, D., Živanović, M., Zečević, Ž., Simić, M., Gobeljić, B., Vujić, D.,& Bugarski, D.. (2022). Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 323.
https://doi.org/10.3390/biom12020323

Borojević A, Jauković A, Kukolj T, Mojsilović S, Obradović H, Trivanović D, Živanović M, Zečević Ž, Simić M, Gobeljić B, Vujić D, Bugarski D. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules. 2022;12(2):323.
doi:10.3390/biom12020323 .

Borojević, Ana, Jauković, Aleksandra, Kukolj, Tamara, Mojsilović, Slavko, Obradović, Hristina, Trivanović, Drenka, Živanović, Milena, Zečević, Željko, Simić, Marija, Gobeljić, Borko, Vujić, Dragana, Bugarski, Diana, "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling" in Biomolecules, 12, no. 2 (2022):323,
https://doi.org/10.3390/biom12020323 . .

TY  - JOUR
AU  - Trivanović, Drenka
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1242
PB  - MDPI
T2  - Journal of Personalized Medicine
T1  - Editorial: Adult Stem Cells in Aging
IS  - 5
SP  - 795
VL  - 12
DO  - 10.3390/jpm12050795
ER  - 

@article{
author = "Trivanović, Drenka",
year = "2022",
publisher = "MDPI",
journal = "Journal of Personalized Medicine",
title = "Editorial: Adult Stem Cells in Aging",
number = "5",
pages = "795",
volume = "12",
doi = "10.3390/jpm12050795"
}

Trivanović, D.. (2022). Editorial: Adult Stem Cells in Aging. in Journal of Personalized Medicine
MDPI., 12(5), 795.
https://doi.org/10.3390/jpm12050795

Trivanović D. Editorial: Adult Stem Cells in Aging. in Journal of Personalized Medicine. 2022;12(5):795.
doi:10.3390/jpm12050795 .

Trivanović, Drenka, "Editorial: Adult Stem Cells in Aging" in Journal of Personalized Medicine, 12, no. 5 (2022):795,
https://doi.org/10.3390/jpm12050795 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Maslovarić, Irina
AU  - Trivanović, Drenka
AU  - Ilić, Vesna
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1274
AB  - Hemoglobin is essential for maintaining cellular bioenergetic homeostasis through its ability to bind and transport oxygen to the tissues. Besides its ability to transport oxygen, hemoglobin within erythrocytes plays an important role in cellular signaling and modulation of the inflammatory response either directly by binding gas molecules (NO, CO, and CO2) or indirectly by acting as their source. Once hemoglobin reaches the extracellular environment, it acquires several secondary functions affecting surrounding cells and tissues. By modulating the cell functions, this macromolecule becomes involved in the etiology and pathophysiology of various diseases. The up-to-date results disclose the impact of extracellular hemoglobin on (i) redox status, (ii) inflammatory state of cells, (iii) proliferation and chemotaxis, (iv) mitochondrial dynamic, (v) chemoresistance and (vi) differentiation. This review pays special attention to applied biomedical research and the use of non-vertebrate and vertebrate extracellular hemoglobin as a promising candidate for hemoglobin-based oxygen carriers, as well as cell culture medium additive. Although recent experimental settings have some limitations, they provide additional insight into the modulatory activity of extracellular hemoglobin in various cellular microenvironments, such as stem or tumor cells niches.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T1  - Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects
IS  - 11
SP  - 1708
VL  - 12
DO  - 10.3390/biom12111708
ER  - 

@article{
author = "Drvenica, Ivana and Stančić, Ana and Maslovarić, Irina and Trivanović, Drenka and Ilić, Vesna",
year = "2022",
abstract = "Hemoglobin is essential for maintaining cellular bioenergetic homeostasis through its ability to bind and transport oxygen to the tissues. Besides its ability to transport oxygen, hemoglobin within erythrocytes plays an important role in cellular signaling and modulation of the inflammatory response either directly by binding gas molecules (NO, CO, and CO2) or indirectly by acting as their source. Once hemoglobin reaches the extracellular environment, it acquires several secondary functions affecting surrounding cells and tissues. By modulating the cell functions, this macromolecule becomes involved in the etiology and pathophysiology of various diseases. The up-to-date results disclose the impact of extracellular hemoglobin on (i) redox status, (ii) inflammatory state of cells, (iii) proliferation and chemotaxis, (iv) mitochondrial dynamic, (v) chemoresistance and (vi) differentiation. This review pays special attention to applied biomedical research and the use of non-vertebrate and vertebrate extracellular hemoglobin as a promising candidate for hemoglobin-based oxygen carriers, as well as cell culture medium additive. Although recent experimental settings have some limitations, they provide additional insight into the modulatory activity of extracellular hemoglobin in various cellular microenvironments, such as stem or tumor cells niches.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules",
title = "Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects",
number = "11",
pages = "1708",
volume = "12",
doi = "10.3390/biom12111708"
}

Drvenica, I., Stančić, A., Maslovarić, I., Trivanović, D.,& Ilić, V.. (2022). Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 12(11), 1708.
https://doi.org/10.3390/biom12111708

Drvenica I, Stančić A, Maslovarić I, Trivanović D, Ilić V. Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects. in Biomolecules. 2022;12(11):1708.
doi:10.3390/biom12111708 .

Drvenica, Ivana, Stančić, Ana, Maslovarić, Irina, Trivanović, Drenka, Ilić, Vesna, "Extracellular Hemoglobin: Modulation of Cellular Functions and Pathophysiological Effects" in Biomolecules, 12, no. 11 (2022):1708,
https://doi.org/10.3390/biom12111708 . .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Schiminski, Annika
AU  - Schlierf, Bianca
AU  - Horas, Konstantin
AU  - Rudert, Maximilian
AU  - Kozjak-Pavlović, Vera
AU  - Riedel, Angela
AU  - Herrmann, Marietta
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1410
PB  - Elsevier
C3  - Abstracts of the 49th (ECTS) European Calcified Tissue Society Congress,  7–10 May 2022, Helsinki, Finland
T1  - Microniche of human bone marrow adipose tissue hosts progenitor cells with specific mitochondrial bioenergetics and adipogenic potential
IS  - 101424
SP  - P149
VL  - 16 S
DO  - 10.1016/j.bonr.2022.101424
ER  - 

@conference{
author = "Trivanović, Drenka and Schiminski, Annika and Schlierf, Bianca and Horas, Konstantin and Rudert, Maximilian and Kozjak-Pavlović, Vera and Riedel, Angela and Herrmann, Marietta",
year = "2022",
publisher = "Elsevier",
journal = "Abstracts of the 49th (ECTS) European Calcified Tissue Society Congress,  7–10 May 2022, Helsinki, Finland",
title = "Microniche of human bone marrow adipose tissue hosts progenitor cells with specific mitochondrial bioenergetics and adipogenic potential",
number = "101424",
pages = "P149",
volume = "16 S",
doi = "10.1016/j.bonr.2022.101424"
}

Trivanović, D., Schiminski, A., Schlierf, B., Horas, K., Rudert, M., Kozjak-Pavlović, V., Riedel, A.,& Herrmann, M.. (2022). Microniche of human bone marrow adipose tissue hosts progenitor cells with specific mitochondrial bioenergetics and adipogenic potential. in Abstracts of the 49th (ECTS) European Calcified Tissue Society Congress,  7–10 May 2022, Helsinki, Finland
Elsevier., 16 S(101424), P149.
https://doi.org/10.1016/j.bonr.2022.101424

Trivanović D, Schiminski A, Schlierf B, Horas K, Rudert M, Kozjak-Pavlović V, Riedel A, Herrmann M. Microniche of human bone marrow adipose tissue hosts progenitor cells with specific mitochondrial bioenergetics and adipogenic potential. in Abstracts of the 49th (ECTS) European Calcified Tissue Society Congress,  7–10 May 2022, Helsinki, Finland. 2022;16 S(101424):P149.
doi:10.1016/j.bonr.2022.101424 .

Trivanović, Drenka, Schiminski, Annika, Schlierf, Bianca, Horas, Konstantin, Rudert, Maximilian, Kozjak-Pavlović, Vera, Riedel, Angela, Herrmann, Marietta, "Microniche of human bone marrow adipose tissue hosts progenitor cells with specific mitochondrial bioenergetics and adipogenic potential" in Abstracts of the 49th (ECTS) European Calcified Tissue Society Congress,  7–10 May 2022, Helsinki, Finland, 16 S, no. 101424 (2022):P149,
https://doi.org/10.1016/j.bonr.2022.101424 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Miletić, Maja
AU  - Petrović, Vanja
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1149
AB  - Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
PB  - Wiley-Blackwell
T2  - Journal of Cellular Physiology
T1  - Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
EP  - 7341
IS  - 11
SP  - 7322
VL  - 236
DO  - 10.1002/jcp.30399
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Obradović, Hristina and Miletić, Maja and Petrović, Vanja and Mojsilović, Slavko and Bugarski, Diana",
year = "2021",
abstract = "Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.",
publisher = "Wiley-Blackwell",
journal = "Journal of Cellular Physiology",
title = "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF",
pages = "7341-7322",
number = "11",
volume = "236",
doi = "10.1002/jcp.30399"
}

Jauković, A., Kukolj, T., Trivanović, D., Okić Đorđević, I., Obradović, H., Miletić, M., Petrović, V., Mojsilović, S.,& Bugarski, D.. (2021). Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology
Wiley-Blackwell., 236(11), 7322-7341.
https://doi.org/10.1002/jcp.30399

Jauković A, Kukolj T, Trivanović D, Okić Đorđević I, Obradović H, Miletić M, Petrović V, Mojsilović S, Bugarski D. Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology. 2021;236(11):7322-7341.
doi:10.1002/jcp.30399 .

Jauković, Aleksandra, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Obradović, Hristina, Miletić, Maja, Petrović, Vanja, Mojsilović, Slavko, Bugarski, Diana, "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF" in Journal of Cellular Physiology, 236, no. 11 (2021):7322-7341,
https://doi.org/10.1002/jcp.30399 . .

TY  - JOUR
AU  - Herrmann, Marietta
AU  - Diederichs, S.
AU  - Melnik, S.
AU  - Riegger, J.
AU  - Trivanović, Drenka
AU  - Li, S.
AU  - Jenei-Lanzl, Z.
AU  - Brenner, R.E.
AU  - Huber-Lang, M.
AU  - Zaucke, F.
AU  - Schildberg, F.A.
AU  - Grässel, S.
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1069
AB  - The incidence of musculoskeletal diseases is steadily increasing with aging of the population. In the past years, extracellular vesicles (EVs) have gained attention in musculoskeletal research. EVs have been associated with various musculoskeletal pathologies as well as suggested as treatment option. EVs play a pivotal role in communication between cells and their environment. Thereby, the EV cargo is highly dependent on their cellular origin. In this review, we summarize putative mechanisms by which EVs can contribute to musculoskeletal tissue homeostasis, regeneration and disease, in particular matrix remodeling and mineralization, pro-angiogenic effects and immunomodulatory activities. Mesenchymal stromal cells (MSCs) present the most frequently used cell source for EV generation for musculoskeletal applications, and herein we discuss how the MSC phenotype can influence the cargo and thus the regenerative potential of EVs. Induced pluripotent stem cell-derived mesenchymal progenitor cells (iMPs) may overcome current limitations of MSCs, and iMP-derived EVs are discussed as an alternative strategy. In the last part of the article, we focus on therapeutic applications of EVs and discuss both practical considerations for EV production and the current state of EV-based therapies.
T2  - Frontiers in Bioengineering & Biotechnology
T1  - Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration
VL  - 8
DO  - 10.3389/fbioe.2020.624096
ER  - 

@article{
author = "Herrmann, Marietta and Diederichs, S. and Melnik, S. and Riegger, J. and Trivanović, Drenka and Li, S. and Jenei-Lanzl, Z. and Brenner, R.E. and Huber-Lang, M. and Zaucke, F. and Schildberg, F.A. and Grässel, S.",
year = "2021",
abstract = "The incidence of musculoskeletal diseases is steadily increasing with aging of the population. In the past years, extracellular vesicles (EVs) have gained attention in musculoskeletal research. EVs have been associated with various musculoskeletal pathologies as well as suggested as treatment option. EVs play a pivotal role in communication between cells and their environment. Thereby, the EV cargo is highly dependent on their cellular origin. In this review, we summarize putative mechanisms by which EVs can contribute to musculoskeletal tissue homeostasis, regeneration and disease, in particular matrix remodeling and mineralization, pro-angiogenic effects and immunomodulatory activities. Mesenchymal stromal cells (MSCs) present the most frequently used cell source for EV generation for musculoskeletal applications, and herein we discuss how the MSC phenotype can influence the cargo and thus the regenerative potential of EVs. Induced pluripotent stem cell-derived mesenchymal progenitor cells (iMPs) may overcome current limitations of MSCs, and iMP-derived EVs are discussed as an alternative strategy. In the last part of the article, we focus on therapeutic applications of EVs and discuss both practical considerations for EV production and the current state of EV-based therapies.",
journal = "Frontiers in Bioengineering & Biotechnology",
title = "Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration",
volume = "8",
doi = "10.3389/fbioe.2020.624096"
}

Herrmann, M., Diederichs, S., Melnik, S., Riegger, J., Trivanović, D., Li, S., Jenei-Lanzl, Z., Brenner, R.E., Huber-Lang, M., Zaucke, F., Schildberg, F.A.,& Grässel, S.. (2021). Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration. in Frontiers in Bioengineering & Biotechnology, 8.
https://doi.org/10.3389/fbioe.2020.624096

Herrmann M, Diederichs S, Melnik S, Riegger J, Trivanović D, Li S, Jenei-Lanzl Z, Brenner R, Huber-Lang M, Zaucke F, Schildberg F, Grässel S. Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration. in Frontiers in Bioengineering & Biotechnology. 2021;8.
doi:10.3389/fbioe.2020.624096 .

Herrmann, Marietta, Diederichs, S., Melnik, S., Riegger, J., Trivanović, Drenka, Li, S., Jenei-Lanzl, Z., Brenner, R.E., Huber-Lang, M., Zaucke, F., Schildberg, F.A., Grässel, S., "Extracellular Vesicles in Musculoskeletal Pathologies and Regeneration" in Frontiers in Bioengineering & Biotechnology, 8 (2021),
https://doi.org/10.3389/fbioe.2020.624096 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Santibanez, Juan F.
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1003
AB  - Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function.
AB  - Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts
T1  - Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta
EP  - 33
IS  - 1
SP  - 18
VL  - 74
DO  - 10.2298/VETGL190414019M
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Stančić, Ana and Santibanez, Juan F. and Trivanović, Drenka and Drvenica, Ivana and Krstić, Jelena and Mojsilović, Slavko and Okić Đorđević, Ivana and Bugarski, Diana",
year = "2020",
abstract = "Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function., Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts, Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta",
pages = "33-18",
number = "1",
volume = "74",
doi = "10.2298/VETGL190414019M"
}

Maslovarić, I., Ilić, V., Stančić, A., Santibanez, J. F., Trivanović, D., Drvenica, I., Krstić, J., Mojsilović, S., Okić Đorđević, I.,& Bugarski, D.. (2020). Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(1), 18-33.
https://doi.org/10.2298/VETGL190414019M

Maslovarić I, Ilić V, Stančić A, Santibanez JF, Trivanović D, Drvenica I, Krstić J, Mojsilović S, Okić Đorđević I, Bugarski D. Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik. 2020;74(1):18-33.
doi:10.2298/VETGL190414019M .

Maslovarić, Irina, Ilić, Vesna, Stančić, Ana, Santibanez, Juan F., Trivanović, Drenka, Drvenica, Ivana, Krstić, Jelena, Mojsilović, Slavko, Okić Đorđević, Ivana, Bugarski, Diana, "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts" in Veterinarski glasnik, 74, no. 1 (2020):18-33,
https://doi.org/10.2298/VETGL190414019M . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Vignjević-Petrinović, Sanja
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/995
AB  - Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cell & Developmental Biology
T1  - Adipogenesis in Different Body Depots and Tumor Development
VL  - 8
DO  - 10.3389/fcell.2020.571648
ER  - 

@article{
author = "Trivanović, Drenka and Vignjević-Petrinović, Sanja and Okić Đorđević, Ivana and Kukolj, Tamara and Bugarski, Diana and Jauković, Aleksandra",
year = "2020",
abstract = "Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cell & Developmental Biology",
title = "Adipogenesis in Different Body Depots and Tumor Development",
volume = "8",
doi = "10.3389/fcell.2020.571648"
}

Trivanović, D., Vignjević-Petrinović, S., Okić Đorđević, I., Kukolj, T., Bugarski, D.,& Jauković, A.. (2020). Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fcell.2020.571648

Trivanović D, Vignjević-Petrinović S, Okić Đorđević I, Kukolj T, Bugarski D, Jauković A. Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology. 2020;8.
doi:10.3389/fcell.2020.571648 .

Trivanović, Drenka, Vignjević-Petrinović, Sanja, Okić Đorđević, Ivana, Kukolj, Tamara, Bugarski, Diana, Jauković, Aleksandra, "Adipogenesis in Different Body Depots and Tumor Development" in Frontiers in Cell & Developmental Biology, 8 (2020),
https://doi.org/10.3389/fcell.2020.571648 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Abadjieva, Desislava
AU  - Trivanović, Drenka
AU  - Stoyanova, Elena
AU  - Kostadinova, Milena
AU  - Pashova, Shina
AU  - Kestendjieva, Snejana
AU  - Kukolj, Tamara
AU  - Ješeta, Michal
AU  - Kistanova, Elena
AU  - Mourdjeva, Milena
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1055
AB  - Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.
PB  - Springer, New York
T2  - Stem Cell Reviews & Reports
T1  - Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties
EP  - 875
IS  - 5
SP  - 853
VL  - 16
DO  - 10.1007/s12015-020-10006-9
ER  - 

@article{
author = "Jauković, Aleksandra and Abadjieva, Desislava and Trivanović, Drenka and Stoyanova, Elena and Kostadinova, Milena and Pashova, Shina and Kestendjieva, Snejana and Kukolj, Tamara and Ješeta, Michal and Kistanova, Elena and Mourdjeva, Milena",
year = "2020",
abstract = "Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.",
publisher = "Springer, New York",
journal = "Stem Cell Reviews & Reports",
title = "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties",
pages = "875-853",
number = "5",
volume = "16",
doi = "10.1007/s12015-020-10006-9"
}

Jauković, A., Abadjieva, D., Trivanović, D., Stoyanova, E., Kostadinova, M., Pashova, S., Kestendjieva, S., Kukolj, T., Ješeta, M., Kistanova, E.,& Mourdjeva, M.. (2020). Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports
Springer, New York., 16(5), 853-875.
https://doi.org/10.1007/s12015-020-10006-9

Jauković A, Abadjieva D, Trivanović D, Stoyanova E, Kostadinova M, Pashova S, Kestendjieva S, Kukolj T, Ješeta M, Kistanova E, Mourdjeva M. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports. 2020;16(5):853-875.
doi:10.1007/s12015-020-10006-9 .

Jauković, Aleksandra, Abadjieva, Desislava, Trivanović, Drenka, Stoyanova, Elena, Kostadinova, Milena, Pashova, Shina, Kestendjieva, Snejana, Kukolj, Tamara, Ješeta, Michal, Kistanova, Elena, Mourdjeva, Milena, "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties" in Stem Cell Reviews & Reports, 16, no. 5 (2020):853-875,
https://doi.org/10.1007/s12015-020-10006-9 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/950
AB  - Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.
PB  - Wiley, Hoboken
T2  - Cell Proliferation
T1  - IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells
IS  - 1
VL  - 52
DO  - 10.1111/cpr.12533
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Mojsilović, Slavko and Okić Đorđević, Ivana and Obradović, Hristina and Krstić, Jelena and Jauković, Aleksandra and Bugarski, Diana",
year = "2019",
abstract = "Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.",
publisher = "Wiley, Hoboken",
journal = "Cell Proliferation",
title = "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells",
number = "1",
volume = "52",
doi = "10.1111/cpr.12533"
}

Kukolj, T., Trivanović, D., Mojsilović, S., Okić Đorđević, I., Obradović, H., Krstić, J., Jauković, A.,& Bugarski, D.. (2019). IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation
Wiley, Hoboken., 52(1).
https://doi.org/10.1111/cpr.12533

Kukolj T, Trivanović D, Mojsilović S, Okić Đorđević I, Obradović H, Krstić J, Jauković A, Bugarski D. IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation. 2019;52(1).
doi:10.1111/cpr.12533 .

Kukolj, Tamara, Trivanović, Drenka, Mojsilović, Slavko, Okić Đorđević, Ivana, Obradović, Hristina, Krstić, Jelena, Jauković, Aleksandra, Bugarski, Diana, "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells" in Cell Proliferation, 52, no. 1 (2019),
https://doi.org/10.1111/cpr.12533 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić, Ivana
AU  - Kukolj, Tamara
AU  - Ilić, Vesna
AU  - Jauković, Aleksandra
AU  - Terzić, Milan
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/969
AB  - Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.
PB  - W B Saunders Co Ltd, London
T2  - Placenta
T1  - Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche
EP  - 34
SP  - 25
VL  - 82
DO  - 10.1016/j.placenta.2019.05.005
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Trivanović, Drenka and Mojsilović, Slavko and Okić, Ivana and Kukolj, Tamara and Ilić, Vesna and Jauković, Aleksandra and Terzić, Milan and Bugarski, Diana",
year = "2019",
abstract = "Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.",
publisher = "W B Saunders Co Ltd, London",
journal = "Placenta",
title = "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche",
pages = "34-25",
volume = "82",
doi = "10.1016/j.placenta.2019.05.005"
}

Obradović, H., Krstić, J., Trivanović, D., Mojsilović, S., Okić, I., Kukolj, T., Ilić, V., Jauković, A., Terzić, M.,& Bugarski, D.. (2019). Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta
W B Saunders Co Ltd, London., 82, 25-34.
https://doi.org/10.1016/j.placenta.2019.05.005

Obradović H, Krstić J, Trivanović D, Mojsilović S, Okić I, Kukolj T, Ilić V, Jauković A, Terzić M, Bugarski D. Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta. 2019;82:25-34.
doi:10.1016/j.placenta.2019.05.005 .

Obradović, Hristina, Krstić, Jelena, Trivanović, Drenka, Mojsilović, Slavko, Okić, Ivana, Kukolj, Tamara, Ilić, Vesna, Jauković, Aleksandra, Terzić, Milan, Bugarski, Diana, "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche" in Placenta, 82 (2019):25-34,
https://doi.org/10.1016/j.placenta.2019.05.005 . .

TY  - JOUR
AU  - Pereira, A. R.
AU  - Trivanović, Drenka
AU  - Herrmann, Marietta
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/939
AB  - Mesenchymal stem/stromal cells (MSCs) are an essential element of most modem tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease - including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.
PB  - Ao Research Institute Davos-Ari, Davos
T2  - European Cells & Materials
T1  - Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro
EP  - 112
SP  - 88
VL  - 37
DO  - 10.22203/eCM.v037a07
ER  - 

@article{
author = "Pereira, A. R. and Trivanović, Drenka and Herrmann, Marietta",
year = "2019",
abstract = "Mesenchymal stem/stromal cells (MSCs) are an essential element of most modem tissue engineering and regenerative medicine approaches due to their multipotency and immunoregulatory functions. Despite the prospective value of MSCs for the clinics, the stem cells community is questioning their developmental origin, in vivo localization, identification, and regenerative potential after several years of far-reaching research in the field. Although several major progresses have been made in mimicking the complexity of the MSC niche in vitro, there is need for comprehensive studies of fundamental mechanisms triggered by microenvironmental cues before moving to regenerative medicine cell therapy applications. The present comprehensive review extensively discusses the microenvironmental cues that influence MSC phenotype and function in health and disease - including cellular, chemical and physical interactions. The most recent and relevant illustrative examples of novel bioengineering approaches to mimic biological, chemical, and mechanical microenvironmental signals present in the native MSC niche are summarized, with special emphasis on the forefront techniques to achieve bio-chemical complexity and dynamic cultures. In particular, the skeletal MSC niche and applications focusing on the bone regenerative potential of MSC are addressed. The aim of the review was to recognize the limitations of the current MSC niche in vitro models and to identify potential opportunities to fill the bridge between fundamental science and clinical application of MSCs.",
publisher = "Ao Research Institute Davos-Ari, Davos",
journal = "European Cells & Materials",
title = "Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro",
pages = "112-88",
volume = "37",
doi = "10.22203/eCM.v037a07"
}

Pereira, A. R., Trivanović, D.,& Herrmann, M.. (2019). Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro. in European Cells & Materials
Ao Research Institute Davos-Ari, Davos., 37, 88-112.
https://doi.org/10.22203/eCM.v037a07

Pereira AR, Trivanović D, Herrmann M. Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro. in European Cells & Materials. 2019;37:88-112.
doi:10.22203/eCM.v037a07 .

Pereira, A. R., Trivanović, Drenka, Herrmann, Marietta, "Approaches to mimic the complexity of the skeletal mesenchymal stem/stromal cell niche in vitro" in European Cells & Materials, 37 (2019):88-112,
https://doi.org/10.22203/eCM.v037a07 . .

TY  - JOUR
AU  - Trivanović, Drenka
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/979
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Stem Cell Research & Therapy
T1  - "Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above
EP  - 292
IS  - 4
SP  - 292
VL  - 14
DO  - 10.2174/1574888X1404190408124422
ER  - 

@article{
author = "Trivanović, Drenka",
year = "2019",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Stem Cell Research & Therapy",
title = ""Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above",
pages = "292-292",
number = "4",
volume = "14",
doi = "10.2174/1574888X1404190408124422"
}

Trivanović, D.. (2019). "Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above. in Current Stem Cell Research & Therapy
Bentham Science Publ Ltd, Sharjah., 14(4), 292-292.
https://doi.org/10.2174/1574888X1404190408124422

Trivanović D. "Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above. in Current Stem Cell Research & Therapy. 2019;14(4):292-292.
doi:10.2174/1574888X1404190408124422 .

Trivanović, Drenka, ""Mesenchymal Stem Cells": Blurred Image of Developmental Origin, Cellular Niche and Traits with Development of Regenerative Therapy Over and Above" in Current Stem Cell Research & Therapy, 14, no. 4 (2019):292-292,
https://doi.org/10.2174/1574888X1404190408124422 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/835
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine & Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine & Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine & Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .