TY  - CHAP
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Bugarski, Branko
AU  - Pajić-Lijaković, Ivana
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/920
AB  - For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.
T2  - Erythrocytes: Structure, Functions & Clinical Aspects
T1  - Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems
EP  - 132
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_technorep_4194
ER  - 

@inbook{
author = "Drvenica, Ivana and Stančić, Ana and Bugarski, Branko and Pajić-Lijaković, Ivana and Maslovarić, Irina and Ilić, Vesna",
year = "2019",
abstract = "For many decades, the red blood cell membranes were in focus of research interest solely as a model system for investigation into the various membrane-related phenomena, composition/organization or membrane transport properties, as well as the comparative proteomic and lipidomic analyses in health and disease. During 50s, along with the first experimental steps in ATP encapsulation in erythrocytes membranes, these entities begin to fascinate clinicians and researchers by their super carrier capabilities for the controlled and targeted delivery (vascular, pulmonary, subcutaneous) of wide range of conventional drugs and biologicals. A relatively new realm for erythrocyte membrane is its application in targeted delivery of nanoparticles, like erythrocyte membrane cloaked nanoparticles, incorporating their most useful traits such as long circulation and stealth features. This chapter focuses on red blood cell membrane as unique constituent of drug delivery systems, including nano-sized ones (nanoerythrosomes) and ex vivo technologies for their preparation. Rheological characterization of membranes as well as the change induced by various experimental conditions is prerequisite for their application as drug carriers. The membrane viscoelasticity described by appropriate constitutive model is related to kinetic of drug release in order to whole process optimization. Furthermore, chapter will bring review of developed hybrid drug delivery vehicles of erythrocyte membranes as natural bio-derivative material, and nanoparticles, mainly made of synthetic material, whose combined advantages serve as immunologically non-invasive drug delivery platform. The advantages and drawbacks are specifically summarized to get critical point of view on existing and future medical applications of erythrocyte membrane as drug carriers. As an example of complexity in research toward development of such erythrocyte membrane based drug delivery systems starting from animal erythrocyte, morphological, biochemical and drug release profile assessment will be reviewed.",
journal = "Erythrocytes: Structure, Functions & Clinical Aspects",
booktitle = "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems",
pages = "132-57",
url = "https://hdl.handle.net/21.15107/rcub_technorep_4194"
}

Drvenica, I., Stančić, A., Bugarski, B., Pajić-Lijaković, I., Maslovarić, I.,& Ilić, V.. (2019). Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions & Clinical Aspects, 57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194

Drvenica I, Stančić A, Bugarski B, Pajić-Lijaković I, Maslovarić I, Ilić V. Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems. in Erythrocytes: Structure, Functions & Clinical Aspects. 2019;:57-132.
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

Drvenica, Ivana, Stančić, Ana, Bugarski, Branko, Pajić-Lijaković, Ivana, Maslovarić, Irina, Ilić, Vesna, "Erythrocyte membranes: Unique constituent of biological/hybri drug delivery systems" in Erythrocytes: Structure, Functions & Clinical Aspects (2019):57-132,
https://hdl.handle.net/21.15107/rcub_technorep_4194 .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Ilić, Vesna
AU  - Bugarski, Branko
AU  - Plavšić, Milenko
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/264
AB  - The complex phenomenon of rearrangement of band 3 molecules after erythrocyte swelling under hypotonic condition is considered. The rearrangement includes the increase of the mobile fraction and clustering of band 3. The self-associative tendency and the action of the elastic field generated within the lipid membrane after erythrocyte swelling result in equilibration of the number of molecules per cluster and the number of clusters. The local perturbation of the elastic field induces excitation of the cluster in the nearest neighbor and changes its packing state generating changes in the free volume within the cluster. The local perturbation could result in the reversible formation of osmotic hole. We formulated a model to predict changes of the cluster packing states generated by rearrangement of band 3 molecules on two time-scales. The phenomenon is examined on the basis of two experimental sets, i.e. low (5.2 mM Na3PO4 solution) and high (46.0 mM Na3PO4 solution) hypotonicities at 21A degrees C, from Golan and Veatch (Proc Natl Acad Sci 77(5):2537-2541, 1980). Modeling considerations suggested that lower hypotonic conditions resulted in higher values of: the driving force of agglomeration of band 3 as a measure of self-associative tendency, the specific rate of cluster breaking, the specific rate of increase of the mobile fraction of band 3, and the dispersion of cluster sizes. Lower hypotonic conditions ensure the generation of a higher average value of the free energy within the membrane after erythrocyte swelling, which enables more intensive rearrangement of band 3 molecules.
PB  - Springer, New York
T2  - European Biophysics Journal with Biophysics Letters
T1  - Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions
EP  - 800
IS  - 5
SP  - 789
VL  - 39
DO  - 10.1007/s00249-009-0554-6
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Ilić, Vesna and Bugarski, Branko and Plavšić, Milenko",
year = "2010",
abstract = "The complex phenomenon of rearrangement of band 3 molecules after erythrocyte swelling under hypotonic condition is considered. The rearrangement includes the increase of the mobile fraction and clustering of band 3. The self-associative tendency and the action of the elastic field generated within the lipid membrane after erythrocyte swelling result in equilibration of the number of molecules per cluster and the number of clusters. The local perturbation of the elastic field induces excitation of the cluster in the nearest neighbor and changes its packing state generating changes in the free volume within the cluster. The local perturbation could result in the reversible formation of osmotic hole. We formulated a model to predict changes of the cluster packing states generated by rearrangement of band 3 molecules on two time-scales. The phenomenon is examined on the basis of two experimental sets, i.e. low (5.2 mM Na3PO4 solution) and high (46.0 mM Na3PO4 solution) hypotonicities at 21A degrees C, from Golan and Veatch (Proc Natl Acad Sci 77(5):2537-2541, 1980). Modeling considerations suggested that lower hypotonic conditions resulted in higher values of: the driving force of agglomeration of band 3 as a measure of self-associative tendency, the specific rate of cluster breaking, the specific rate of increase of the mobile fraction of band 3, and the dispersion of cluster sizes. Lower hypotonic conditions ensure the generation of a higher average value of the free energy within the membrane after erythrocyte swelling, which enables more intensive rearrangement of band 3 molecules.",
publisher = "Springer, New York",
journal = "European Biophysics Journal with Biophysics Letters",
title = "Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions",
pages = "800-789",
number = "5",
volume = "39",
doi = "10.1007/s00249-009-0554-6"
}

Pajić-Lijaković, I., Ilić, V., Bugarski, B.,& Plavšić, M.. (2010). Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions. in European Biophysics Journal with Biophysics Letters
Springer, New York., 39(5), 789-800.
https://doi.org/10.1007/s00249-009-0554-6

Pajić-Lijaković I, Ilić V, Bugarski B, Plavšić M. Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions. in European Biophysics Journal with Biophysics Letters. 2010;39(5):789-800.
doi:10.1007/s00249-009-0554-6 .

Pajić-Lijaković, Ivana, Ilić, Vesna, Bugarski, Branko, Plavšić, Milenko, "Rearrangement of erythrocyte band 3 molecules and reversible formation of osmotic holes under hypotonic conditions" in European Biophysics Journal with Biophysics Letters, 39, no. 5 (2010):789-800,
https://doi.org/10.1007/s00249-009-0554-6 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Bugarski, Diana
AU  - Plavšić, Milenko
AU  - Bugarski, Branko
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/179
AB  - A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.
PB  - Elsevier Sci Ltd, Oxford
T2  - Process Biochemistry
T1  - Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule
EP  - 174
IS  - 2
SP  - 167
VL  - 42
DO  - 10.1016/j.procbio.2006.07.023
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Bugarski, Diana and Plavšić, Milenko and Bugarski, Branko",
year = "2007",
abstract = "A mathematical model was formulated to describe hybridoma cell growth within the alginate-poly-L-lysine (alginate-PLL) microcapsules during air-lift bioreactor cultivation. Model development was based on experimentally obtained data concerning the hybridoma cell counts, monoclonal antibody (mAb) production and the distribution of hybridoma cell growth within the microcapsules. The cell growth was modeled using a mean field approach expressed as Langevin class of equations for two different regions of alginate-PLL microcapsules, the alginate microcapsule core and the annular region between microcapsule core and membrane. In this paper we propose an influence of microenvironmental conditions on cell growth. The osmotic pressure changes in the Na-alginate liquefied annular region, as well as, the resistance effects of Ca-alginate hydrogel in the core region during the cell growth were incorporated into the model. Good agreement between the experimental data and model prediction values was obtained. The proposed model successfully predicted the impact of various microenvironmental restriction effects on the dynamics of cell growth and appears useful for further optimization of microcapsule design in order to achieve higher intra-capsule cell concentrations resulting in higher amounts of mAb produced.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Process Biochemistry",
title = "Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule",
pages = "174-167",
number = "2",
volume = "42",
doi = "10.1016/j.procbio.2006.07.023"
}

Pajić-Lijaković, I., Bugarski, D., Plavšić, M.,& Bugarski, B.. (2007). Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. in Process Biochemistry
Elsevier Sci Ltd, Oxford., 42(2), 167-174.
https://doi.org/10.1016/j.procbio.2006.07.023

Pajić-Lijaković I, Bugarski D, Plavšić M, Bugarski B. Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule. in Process Biochemistry. 2007;42(2):167-174.
doi:10.1016/j.procbio.2006.07.023 .

Pajić-Lijaković, Ivana, Bugarski, Diana, Plavšić, Milenko, Bugarski, Branko, "Influence of microenvironmental conditions on hybridoma cell growth inside the alginate-poly-L-lysine microcapsule" in Process Biochemistry, 42, no. 2 (2007):167-174,
https://doi.org/10.1016/j.procbio.2006.07.023 . .

TY  - JOUR
AU  - Pajić-Lijaković, Ivana
AU  - Bugarski, Branko
AU  - Obradović, Bojana
AU  - Plavšić, Milenko
AU  - Bugarski, Diana
PY  - 2003
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/84
AB  - Development of lipid-based fine particles as potential drug carriers requires detailed investigation of possible effects of these carriers on rheological properties of blood. In this study, we have investigated the influence of dynamic conditions on aggregate formation and stability in dispersions of lipid-based fine particles in whole blood under in vitro conditions. Rheological parameters of two concentrations of liposome dispersion and two concentrations of lipid emulsion in blood were studied by assessing shear stress/shear rate relationships. The magnitude of attractive interactions between aggregates and/or particles, A, and the effective-to-real volume fraction of particles, phi(f)/phi(p) , were estimated for rheological quantification of lipid-based fine particles-blood interactions and aggregate stability. Addition of lipid-based particles induced aggregate formation in blood, which was more pronounced at higher concentrations of lipid-based fine particles. Furthermore, larger and more stable aggregates were formed in liposome dispersions as compared to lipid emulsions in blood.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Chemical Engineering Communications
T1  - Examination of rheological properties of fine particles as carriers for controlled drug release
EP  - 93
IS  - 1
SP  - 83
VL  - 190
DO  - 10.1080/00986440302091
ER  - 

@article{
author = "Pajić-Lijaković, Ivana and Bugarski, Branko and Obradović, Bojana and Plavšić, Milenko and Bugarski, Diana",
year = "2003",
abstract = "Development of lipid-based fine particles as potential drug carriers requires detailed investigation of possible effects of these carriers on rheological properties of blood. In this study, we have investigated the influence of dynamic conditions on aggregate formation and stability in dispersions of lipid-based fine particles in whole blood under in vitro conditions. Rheological parameters of two concentrations of liposome dispersion and two concentrations of lipid emulsion in blood were studied by assessing shear stress/shear rate relationships. The magnitude of attractive interactions between aggregates and/or particles, A, and the effective-to-real volume fraction of particles, phi(f)/phi(p) , were estimated for rheological quantification of lipid-based fine particles-blood interactions and aggregate stability. Addition of lipid-based particles induced aggregate formation in blood, which was more pronounced at higher concentrations of lipid-based fine particles. Furthermore, larger and more stable aggregates were formed in liposome dispersions as compared to lipid emulsions in blood.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Chemical Engineering Communications",
title = "Examination of rheological properties of fine particles as carriers for controlled drug release",
pages = "93-83",
number = "1",
volume = "190",
doi = "10.1080/00986440302091"
}

Pajić-Lijaković, I., Bugarski, B., Obradović, B., Plavšić, M.,& Bugarski, D.. (2003). Examination of rheological properties of fine particles as carriers for controlled drug release. in Chemical Engineering Communications
Taylor & Francis Inc, Philadelphia., 190(1), 83-93.
https://doi.org/10.1080/00986440302091

Pajić-Lijaković I, Bugarski B, Obradović B, Plavšić M, Bugarski D. Examination of rheological properties of fine particles as carriers for controlled drug release. in Chemical Engineering Communications. 2003;190(1):83-93.
doi:10.1080/00986440302091 .

Pajić-Lijaković, Ivana, Bugarski, Branko, Obradović, Bojana, Plavšić, Milenko, Bugarski, Diana, "Examination of rheological properties of fine particles as carriers for controlled drug release" in Chemical Engineering Communications, 190, no. 1 (2003):83-93,
https://doi.org/10.1080/00986440302091 . .