TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1038
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić V, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - THES
AU  - Stančić, Ana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1139
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7760
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22991/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=24874761
UR  - https://nardus.mpn.gov.rs/handle/123456789/17637
AB  - Истраживања обухваћена овом докторском дисертацијом имала су за циљ добијање података о утицају ванћелијског хемоглобина на функцијска својства мезенхимских ћелија in vitro. Свињски и говеђи хемоглобин коришћени су као модел ксеногених, лако доступних хемоглобина, који показују висок степен хомологије са хуманим хемоглобином. Као адекватни ћелијски модел системи одабрани су леукоцити периферне крви човека и мезенхимске матичне ћелије периферне крви човека (ПК-ММЋ), као ћелије са којима ванћелијски хемоглобин може доћи у контакт при инстраваскуларној хемолизи. Додатно, утицај хемоглобина испитан је и на четири ћелијске линије ATDC5, MC3T3-E1, 3T3-L1 и C2C12 које представљају униформније модел системе у односу на ПК-ММЋ за проучавање хондро-, остео-, адипо- и миогенезе. Добијени резултати показују да ванћелијски хемоглобин има значајан утицај на функцијска својства мезенхимских ћелија, који зависи од врсте организма из ког је хемоглобин изолован, примењене концентрације и типа ћелија на којима је ефекат испитиван. Ванћелијски хемоглобин модулише вијабилност, способност адхезије и продукције реактивних врста кисеоника испитиваних леукоцита, као и пролиферацију/диференцијацију опредељених матичних ћелија хематопоезе. Додатно, показано је да ванћелијски хемоглобин у концентрацији од 0,1 μM остварује ефекат на вијабилност, миграторни и имуномодулаторни капацитет мезенхимских ћелија и инхибира њихову диференцијацију ка хондрогеној, остеогеној, адипогеној и миогеној линији, модулишући експресију специфичних генских маркера. Ови резултати доприносе бољем сагледавању утицаја ванћелијског хемоглобина у условима интраваскуларне хемолизе на физиологију мезенхимских ћелија, као и његове потенцијалне примене као дијететског суплемента или адитива медијума за култивацију ћелија.
AB  - The  main  goal  of  this  doctoral  dissertation  was  the  investigation  of  the  effect  of extracellular hemoglobin on the functional properties of mesenchymal cells in vitro. Porcine and bovine hemoglobin were used as models of xenogeneic, readily available hemoglobins, showing a  high  degree  of  homology  with  human  hemoglobin.  Human  peripheral  blood  leukocytes  and human peripheral blood mesenchymal stem cells (PB-MSCs) were selected as adequate cell model systems,   since   extracellular   hemoglobin   may   come   into   contact   with   these   cells   during instravascular hemolysis. In addition, the effects of hemoglobin were also tested on four cell lines ATDC5, MC3T3-E1, 3T3-L1, and C2C12 as more uniform model systems compared to PB-MSCs to study chondro-, osteo-, adipo-, and myogenesis.The obtained results show that extracellular hemoglobin has major effect on the functional properties  of  mesenchymal  cells,which  depends  on  the  species  of  organism  from  which hemoglobin was isolated, hemoglobin concentration used and the type of cells tested. Extracellular hemoglobin  modulates  viability,  adhesion  and  synthesis  ability  of  reactive  oxygen  species  of leukocytes examined,  as  well  as  proliferation/differentiation  of  committed  hematopoietic  stem cells.  Furthermore,  at  a  concentration  of  0.1  μM  extracellular  hemoglobin  had  the  effect  on viability,  migratory  and  immunomodulatory  capacity  of  mesenchymal  cells  and  inhibited  their differentiation toward chondro-, osteo-, adipo-and myogenic lineages, modulating the expression of specific gene markers.These results contribute to a better understanding of the effects of extracellular hemoglobin during  intravascular  hemolysis  on  the  physiology  of  mesenchymal  cells,  as  well  as  its  potential applications as a dietary supplement or cell culture media additive.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Утицај ванћелијског хемоглобина ксеногеног порекла на функцијска својства мезенхимских ћелија in vitro
T1  - Effect of extracellular hemoglobin of xenogeneic origin on functional characteristics of mesenchymal cells in vitro
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17637
ER  - 

@phdthesis{
author = "Stančić, Ana",
year = "2020",
abstract = "Истраживања обухваћена овом докторском дисертацијом имала су за циљ добијање података о утицају ванћелијског хемоглобина на функцијска својства мезенхимских ћелија in vitro. Свињски и говеђи хемоглобин коришћени су као модел ксеногених, лако доступних хемоглобина, који показују висок степен хомологије са хуманим хемоглобином. Као адекватни ћелијски модел системи одабрани су леукоцити периферне крви човека и мезенхимске матичне ћелије периферне крви човека (ПК-ММЋ), као ћелије са којима ванћелијски хемоглобин може доћи у контакт при инстраваскуларној хемолизи. Додатно, утицај хемоглобина испитан је и на четири ћелијске линије ATDC5, MC3T3-E1, 3T3-L1 и C2C12 које представљају униформније модел системе у односу на ПК-ММЋ за проучавање хондро-, остео-, адипо- и миогенезе. Добијени резултати показују да ванћелијски хемоглобин има значајан утицај на функцијска својства мезенхимских ћелија, који зависи од врсте организма из ког је хемоглобин изолован, примењене концентрације и типа ћелија на којима је ефекат испитиван. Ванћелијски хемоглобин модулише вијабилност, способност адхезије и продукције реактивних врста кисеоника испитиваних леукоцита, као и пролиферацију/диференцијацију опредељених матичних ћелија хематопоезе. Додатно, показано је да ванћелијски хемоглобин у концентрацији од 0,1 μM остварује ефекат на вијабилност, миграторни и имуномодулаторни капацитет мезенхимских ћелија и инхибира њихову диференцијацију ка хондрогеној, остеогеној, адипогеној и миогеној линији, модулишући експресију специфичних генских маркера. Ови резултати доприносе бољем сагледавању утицаја ванћелијског хемоглобина у условима интраваскуларне хемолизе на физиологију мезенхимских ћелија, као и његове потенцијалне примене као дијететског суплемента или адитива медијума за култивацију ћелија., The  main  goal  of  this  doctoral  dissertation  was  the  investigation  of  the  effect  of extracellular hemoglobin on the functional properties of mesenchymal cells in vitro. Porcine and bovine hemoglobin were used as models of xenogeneic, readily available hemoglobins, showing a  high  degree  of  homology  with  human  hemoglobin.  Human  peripheral  blood  leukocytes  and human peripheral blood mesenchymal stem cells (PB-MSCs) were selected as adequate cell model systems,   since   extracellular   hemoglobin   may   come   into   contact   with   these   cells   during instravascular hemolysis. In addition, the effects of hemoglobin were also tested on four cell lines ATDC5, MC3T3-E1, 3T3-L1, and C2C12 as more uniform model systems compared to PB-MSCs to study chondro-, osteo-, adipo-, and myogenesis.The obtained results show that extracellular hemoglobin has major effect on the functional properties  of  mesenchymal  cells,which  depends  on  the  species  of  organism  from  which hemoglobin was isolated, hemoglobin concentration used and the type of cells tested. Extracellular hemoglobin  modulates  viability,  adhesion  and  synthesis  ability  of  reactive  oxygen  species  of leukocytes examined,  as  well  as  proliferation/differentiation  of  committed  hematopoietic  stem cells.  Furthermore,  at  a  concentration  of  0.1  μM  extracellular  hemoglobin  had  the  effect  on viability,  migratory  and  immunomodulatory  capacity  of  mesenchymal  cells  and  inhibited  their differentiation toward chondro-, osteo-, adipo-and myogenic lineages, modulating the expression of specific gene markers.These results contribute to a better understanding of the effects of extracellular hemoglobin during  intravascular  hemolysis  on  the  physiology  of  mesenchymal  cells,  as  well  as  its  potential applications as a dietary supplement or cell culture media additive.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Утицај ванћелијског хемоглобина ксеногеног порекла на функцијска својства мезенхимских ћелија in vitro, Effect of extracellular hemoglobin of xenogeneic origin on functional characteristics of mesenchymal cells in vitro",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17637"
}

Stančić, A.. (2020). Утицај ванћелијског хемоглобина ксеногеног порекла на функцијска својства мезенхимских ћелија in vitro. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_17637

Stančić A. Утицај ванћелијског хемоглобина ксеногеног порекла на функцијска својства мезенхимских ћелија in vitro. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_17637 .

Stančić, Ana, "Утицај ванћелијског хемоглобина ксеногеног порекла на функцијска својства мезенхимских ћелија in vitro" (2020),
https://hdl.handle.net/21.15107/rcub_nardus_17637 .

TY  - JOUR
AU  - Stojić, Milica
AU  - Ilić, Vesna
AU  - Kovačić, Marijana
AU  - Gvozdić, Dragan
AU  - Stajković, Silvana
AU  - Vejnović, Branislav
AU  - Savić, Olivera
AU  - Fratrić, Natalija
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1017
AB  - This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows
EP  - 435
IS  - 4
SP  - 429
VL  - 87
DO  - 10.1017/S0022029920001077
ER  - 

@article{
author = "Stojić, Milica and Ilić, Vesna and Kovačić, Marijana and Gvozdić, Dragan and Stajković, Silvana and Vejnović, Branislav and Savić, Olivera and Fratrić, Natalija",
year = "2020",
abstract = "This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows",
pages = "435-429",
number = "4",
volume = "87",
doi = "10.1017/S0022029920001077"
}

Stojić, M., Ilić, V., Kovačić, M., Gvozdić, D., Stajković, S., Vejnović, B., Savić, O.,& Fratrić, N.. (2020). Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research
Cambridge Univ Press, New York., 87(4), 429-435.
https://doi.org/10.1017/S0022029920001077

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research. 2020;87(4):429-435.
doi:10.1017/S0022029920001077 .

Stojić, Milica, Ilić, Vesna, Kovačić, Marijana, Gvozdić, Dragan, Stajković, Silvana, Vejnović, Branislav, Savić, Olivera, Fratrić, Natalija, "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows" in Journal of Dairy Research, 87, no. 4 (2020):429-435,
https://doi.org/10.1017/S0022029920001077 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Kalušević, Ana
AU  - Marković, Smilja B.
AU  - Dragišić-Maksimović, Jelena J.
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Ilić, Vesna
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/932
AB  - Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin
EP  - 1117
IS  - 10
SP  - 1105
VL  - 84
DO  - 10.2298/JSC190513067D
ER  - 

@article{
author = "Drvenica, Ivana and Stančić, Ana and Kalušević, Ana and Marković, Smilja B. and Dragišić-Maksimović, Jelena J. and Nedović, Viktor and Bugarski, Branko and Ilić, Vesna",
year = "2019",
abstract = "Slaughterhouse blood represents a valuable source of hemoglobin, which can be used in the production of heme-iron based supplements for the prevention/treatment of iron-deficiency anemia. In order to obtain a stable solid-state formulation, the effect of maltose addition (30 %) on the stability and storage of bovine and porcine hemoglobin in powders obtained by spray-and freeze-drying (without maltose: Hb; with maltose: HbM) were investigated. Differential scanning calorimetry of spray- and freeze-dried powders indicated satisfying quality of the formulation prepared with maltose on dissolving back into solution. After two-year storage at room temperature (20 +/- 5 degrees C) in solid forms, protected from moisture and light, rehydrated spray- and freeze- dried HbM were red, while Hb were brown. Dynamic light scattering showed the presence of native hemoglobin monomers in rehydrated spray- and freeze- dried HbM, but their agglomerates in Hb samples. UV-Vis spectrophotometry confirmed an absence of significant hemoglobin denaturation and methemoglobin formation in HbM freeze-dried powders. In spray-dried HbM, an increased level of methemoglobin was detected. The results confirmed the stabilizing effect of maltose, and suggested its use in the production of long-term stable solid-state formulations of hemoglobin, along with drying processes optimization.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin",
pages = "1117-1105",
number = "10",
volume = "84",
doi = "10.2298/JSC190513067D"
}

Drvenica, I., Stančić, A., Kalušević, A., Marković, S. B., Dragišić-Maksimović, J. J., Nedović, V., Bugarski, B.,& Ilić, V.. (2019). Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 84(10), 1105-1117.
https://doi.org/10.2298/JSC190513067D

Drvenica I, Stančić A, Kalušević A, Marković SB, Dragišić-Maksimović JJ, Nedović V, Bugarski B, Ilić V. Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin. in Journal of the Serbian Chemical Society. 2019;84(10):1105-1117.
doi:10.2298/JSC190513067D .

Drvenica, Ivana, Stančić, Ana, Kalušević, Ana, Marković, Smilja B., Dragišić-Maksimović, Jelena J., Nedović, Viktor, Bugarski, Branko, Ilić, Vesna, "Maltose-mediated, long-term stabilization of freeze- and spray-dried forms of bovine and porcine hemoglobin" in Journal of the Serbian Chemical Society, 84, no. 10 (2019):1105-1117,
https://doi.org/10.2298/JSC190513067D . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/960
AB  - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
EP  - 390
SP  - 384
VL  - 213
DO  - 10.1016/j.saa.2019.01.069
ER  - 

@article{
author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy",
pages = "390-384",
volume = "213",
doi = "10.1016/j.saa.2019.01.069"
}

Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390.
https://doi.org/10.1016/j.saa.2019.01.069

Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;213:384-390.
doi:10.1016/j.saa.2019.01.069 .

Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 213 (2019):384-390,
https://doi.org/10.1016/j.saa.2019.01.069 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Ralević, U.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/966
AB  - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
EP  - 178
SP  - 173
VL  - 216
DO  - 10.1016/j.saa.2019.03.012
ER  - 

@article{
author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy",
pages = "178-173",
volume = "216",
doi = "10.1016/j.saa.2019.03.012"
}

Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178.
https://doi.org/10.1016/j.saa.2019.03.012

Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;216:173-178.
doi:10.1016/j.saa.2019.03.012 .

Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 216 (2019):173-178,
https://doi.org/10.1016/j.saa.2019.03.012 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Mihailović, Marija
AU  - Čudina, Olivera
AU  - Bugarski, Diana
AU  - Ilić, Vesna
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/884
AB  - The biological activity of three previously synthesized 17 beta-carboxamide glucocorticoids (BG, BEG, and MPEA) was tested in vitro on mitogen stimulated and non-stimulated peripheral blood mononuclear cells (MNCs) and granulocytes from human healthy donors, and the results were compared to the conventional glucocorticoid dexamethasone. The tested 17 beta-carboxamide glucocorticoids did not induce decreases in MNC viability and proliferation, while modulation of reactive oxygen species (ROS) synthesis in granulocytes was dependent on the cell donor. The obtained results indicate the possibility of avoidance of strong lymphocyte suppression, which is generally recognized during administration of conventional glucocorticoids. Furthermore, the metabolism of the tested derivatives was predicted in silico. The predicted metabolites were synthesized and the in silico results were confirmed by in vitro evaluation of the metabolism of BG, BEG, and MPEA in human serum and in cultures of peripheral blood MNCs. The results of the biological activity and metabolism evaluation and of previous in vivo evaluations of biological activity indicate the soft drug nature of BG, BEG, and MPEA. In order to be fully considered as soft glucocorticoids, further investigations on the toxicity and activity of the formed metabolites are required.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Archiv der Pharmazie
T1  - Investigation of metabolic properties and effects of 17 beta-carboxamide glucocorticoids on human peripheral blood leukocytes
IS  - 5
SP  - 1700371
VL  - 351
DO  - 10.1002/ardp.201700371
ER  - 

@article{
author = "Dobričić, Vladimir and Drvenica, Ivana and Stančić, Ana and Mihailović, Marija and Čudina, Olivera and Bugarski, Diana and Ilić, Vesna",
year = "2018",
abstract = "The biological activity of three previously synthesized 17 beta-carboxamide glucocorticoids (BG, BEG, and MPEA) was tested in vitro on mitogen stimulated and non-stimulated peripheral blood mononuclear cells (MNCs) and granulocytes from human healthy donors, and the results were compared to the conventional glucocorticoid dexamethasone. The tested 17 beta-carboxamide glucocorticoids did not induce decreases in MNC viability and proliferation, while modulation of reactive oxygen species (ROS) synthesis in granulocytes was dependent on the cell donor. The obtained results indicate the possibility of avoidance of strong lymphocyte suppression, which is generally recognized during administration of conventional glucocorticoids. Furthermore, the metabolism of the tested derivatives was predicted in silico. The predicted metabolites were synthesized and the in silico results were confirmed by in vitro evaluation of the metabolism of BG, BEG, and MPEA in human serum and in cultures of peripheral blood MNCs. The results of the biological activity and metabolism evaluation and of previous in vivo evaluations of biological activity indicate the soft drug nature of BG, BEG, and MPEA. In order to be fully considered as soft glucocorticoids, further investigations on the toxicity and activity of the formed metabolites are required.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Archiv der Pharmazie",
title = "Investigation of metabolic properties and effects of 17 beta-carboxamide glucocorticoids on human peripheral blood leukocytes",
number = "5",
pages = "1700371",
volume = "351",
doi = "10.1002/ardp.201700371"
}

Dobričić, V., Drvenica, I., Stančić, A., Mihailović, M., Čudina, O., Bugarski, D.,& Ilić, V.. (2018). Investigation of metabolic properties and effects of 17 beta-carboxamide glucocorticoids on human peripheral blood leukocytes. in Archiv der Pharmazie
Wiley-V C H Verlag Gmbh, Weinheim., 351(5), 1700371.
https://doi.org/10.1002/ardp.201700371

Dobričić V, Drvenica I, Stančić A, Mihailović M, Čudina O, Bugarski D, Ilić V. Investigation of metabolic properties and effects of 17 beta-carboxamide glucocorticoids on human peripheral blood leukocytes. in Archiv der Pharmazie. 2018;351(5):1700371.
doi:10.1002/ardp.201700371 .

Dobričić, Vladimir, Drvenica, Ivana, Stančić, Ana, Mihailović, Marija, Čudina, Olivera, Bugarski, Diana, Ilić, Vesna, "Investigation of metabolic properties and effects of 17 beta-carboxamide glucocorticoids on human peripheral blood leukocytes" in Archiv der Pharmazie, 351, no. 5 (2018):1700371,
https://doi.org/10.1002/ardp.201700371 . .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Savikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/846
AB  - Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Phytomedicine
T1  - Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats
EP  - 45
SP  - 39
VL  - 46
DO  - 10.1016/j.phymed.2018.04.037
ER  - 

@article{
author = "Vajić, Una Jovana and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Ivanov, Milan and Karanović, Danijela and Savikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2018",
abstract = "Background: Urtica dioica L. (Stinging nettle) has been used for centuries for the treatment of numerous health issues. Purpose: This study investigates the antioxidant capacity and the most abundant phenolic compounds of Urtica dioica L. leaf extract (UE), and its antihypertensive and antioxidative effects in vivo. Study design: Spontaneously hypertensive rats were supplemented with 10, 50, and 200 mg/kg/day of UE and 10 mg/kg/day of losartan during 4-week period. Methods: In this study, HPLC analysis of UE was performed, as well as the determination of antioxidant capacity, superoxide radical scavenging activity, and metal chelating ability. Hemodynamic parameters were measured directly in anesthetized rats. Also, antioxidant enzyme activity and concentration in erythrocytes were determined, as well as systemic oxidative stress and plasma antioxidant status. Results: UE showed higher ferric reducing antioxidant power and Trolox equivalent antioxidant capacity than BHT, but lower than vitamin C. Furthermore, UE showed good metal chelating ability, but weak superoxide radical scavenging activity. All three tested UE doses managed to reduce systolic and diastolic blood pressure, as well as cardiac index, and to improve the antioxidative defense by increasing the activity of superoxide dismutase and catalase, without changing the concentration of the enzymes. Moreover, UE supplementation increased plasma antioxidant capacity and reduced systemic oxidative stress. Conclusion: Chronic UE dietary supplementation had beneficial effects in the experimental model of essential hypertension.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Phytomedicine",
title = "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats",
pages = "45-39",
volume = "46",
doi = "10.1016/j.phymed.2018.04.037"
}

Vajić, U. J., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Ivanov, M., Karanović, D., Savikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2018). Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 46, 39-45.
https://doi.org/10.1016/j.phymed.2018.04.037

Vajić UJ, Grujić-Milanović J, Miloradović Z, Jovović Đ, Ivanov M, Karanović D, Savikin K, Bugarski B, Mihailović-Stanojević N. Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats. in Phytomedicine. 2018;46:39-45.
doi:10.1016/j.phymed.2018.04.037 .

Vajić, Una Jovana, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Ivanov, Milan, Karanović, Danijela, Savikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Urtica dioica L. leaf extract modulates blood pressure and oxidative stress in spontaneously hypertensive rats" in Phytomedicine, 46 (2018):39-45,
https://doi.org/10.1016/j.phymed.2018.04.037 . .

Vajić, U. J.. (2018). Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 81-84.
https://doi.org/10.5937/leksir1838081V

Vajić UJ. Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension. in Lekovite sirovine. 2018;(38):81-84.
doi:10.5937/leksir1838081V .

Vajić, Una Jovana, "Optimization of extraction and characterization of Urtica dioica L. leaf extract for the evaluation of dose-dependent response in experimental hypertension" in Lekovite sirovine, no. 38 (2018):81-84,
https://doi.org/10.5937/leksir1838081V . .

TY  - JOUR
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Grujić-Milanović, Jelica
AU  - Karanović, Danijela
AU  - Dekanski, Dragana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/876
AB  - Haemodynamic alterations in carotid and renal arteries are associated with the severity of target organ damage in patients with hypertension. Dietary habits, such as the Mediterranean diet, regulate blood pressure and oxidative stress, thus reduce the mortality rate due to cardiovascular diseases. In this study, our aim was to evaluate the reducing activity, antioxidant capacity and metal chelating ability of standardized Olea europaea L. leaf extract (OLE), and to test its (5, 25, 50 mg/kg) acute in vivo effects, as well as oleuropein's (OP, 10 mg/kg) on oxidative stress, carotid, renal and systemic haemodynamic parameters (blood pressure, heart rate, cardiac output, peripheral resistance) in spontaneously hypertensive rats (SHR). OLE has a higher antioxidative capacity than BHT, higher reducing ability than vitamin C, and 23 times lower capacity for metal ion chelation than EDTA. All three doses of OLE, and OP, improved oxidative stress in SHR. OLE5 improved carotid and renal haemodynamics, without significant effects on systemic haemodynamics. Two different mechanisms of antihypertensive responses to OLE were observed, OLE25 was most effective in reducing cardiovascular risks by improving systemic and regional (carotid and renal) haemodynamics, peripheral and regional vascular resistance. OLE50 causes the improvement of blood pressure and cardiac performances, but tends to retain elevated vascular resistance, therefore, reducing the inflow of blood into the brain and kidneys of the SHR. The OP did not alter systemic or regional haemodynamics, suggesting others constituents responsible for changes of cardiac function, as well as carotid and renal haemodynamics in response to OLE50.
PB  - Excli Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein
EP  - 44
SP  - 29
VL  - 17
DO  - 10.17179/excli2017-1002
ER  - 

@article{
author = "Ivanov, Milan and Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Jovović, Đurđica and Grujić-Milanović, Jelica and Karanović, Danijela and Dekanski, Dragana",
year = "2018",
abstract = "Haemodynamic alterations in carotid and renal arteries are associated with the severity of target organ damage in patients with hypertension. Dietary habits, such as the Mediterranean diet, regulate blood pressure and oxidative stress, thus reduce the mortality rate due to cardiovascular diseases. In this study, our aim was to evaluate the reducing activity, antioxidant capacity and metal chelating ability of standardized Olea europaea L. leaf extract (OLE), and to test its (5, 25, 50 mg/kg) acute in vivo effects, as well as oleuropein's (OP, 10 mg/kg) on oxidative stress, carotid, renal and systemic haemodynamic parameters (blood pressure, heart rate, cardiac output, peripheral resistance) in spontaneously hypertensive rats (SHR). OLE has a higher antioxidative capacity than BHT, higher reducing ability than vitamin C, and 23 times lower capacity for metal ion chelation than EDTA. All three doses of OLE, and OP, improved oxidative stress in SHR. OLE5 improved carotid and renal haemodynamics, without significant effects on systemic haemodynamics. Two different mechanisms of antihypertensive responses to OLE were observed, OLE25 was most effective in reducing cardiovascular risks by improving systemic and regional (carotid and renal) haemodynamics, peripheral and regional vascular resistance. OLE50 causes the improvement of blood pressure and cardiac performances, but tends to retain elevated vascular resistance, therefore, reducing the inflow of blood into the brain and kidneys of the SHR. The OP did not alter systemic or regional haemodynamics, suggesting others constituents responsible for changes of cardiac function, as well as carotid and renal haemodynamics in response to OLE50.",
publisher = "Excli Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein",
pages = "44-29",
volume = "17",
doi = "10.17179/excli2017-1002"
}

Ivanov, M., Vajić, U. J., Mihailović-Stanojević, N., Miloradović, Z., Jovović, Đ., Grujić-Milanović, J., Karanović, D.,& Dekanski, D.. (2018). Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein. in EXCLI Journal
Excli Journal Managing Office, Dortmund., 17, 29-44.
https://doi.org/10.17179/excli2017-1002

Ivanov M, Vajić UJ, Mihailović-Stanojević N, Miloradović Z, Jovović Đ, Grujić-Milanović J, Karanović D, Dekanski D. Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein. in EXCLI Journal. 2018;17:29-44.
doi:10.17179/excli2017-1002 .

Ivanov, Milan, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Jovović, Đurđica, Grujić-Milanović, Jelica, Karanović, Danijela, Dekanski, Dragana, "Highly potent antioxidant olea europaea l. Leaf extract affects carotid and renal haemodynamics in experimental hypertension: the role of oleuropein" in EXCLI Journal, 17 (2018):29-44,
https://doi.org/10.17179/excli2017-1002 . .

TY  - JOUR
AU  - Ćujić, Nada
AU  - Savikin, Katarina
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Jovović, Đurđica
AU  - Mihailović-Stanojević, Nevena
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/894
AB  - The potential beneficial effects of chronic consumption of dried chokeberry fruit extract (DCE) were evaluated in the present in vivo study. The study concentrates on haemodynamic parameters, lipid profile and oxidative stress in spontaneously hypertensive rats. DCE was characterized by HPLC/DAD method and individual anthocyanins, phenolic acids, and flavonoids were determined. Four week administration of DCE, rich in polyphenols, especially anthocyanins, significantly reduced systolic (P  lt  0.05), pulse pressure (P  lt  0.05) compared to control group, probably via significantly increased diuresis (P  lt  0.05). Significant decrease of plasma and erythrocytes TBARS was also found in the treated group. DCE consumption reduced lipid peroxidation through improving plasma FRAP reducing (P  lt  0.01). The activity of SOD in the treated group had been significantly lower (P  lt  0.01) compared to the control group. These results suggest that DCE could exert beneficial effects on cardiovascular health and has a potential as a nutritional supplement in arterial hypertension.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats
EP  - 339
SP  - 330
VL  - 44
DO  - 10.1016/j.jff.2018.02.027
ER  - 

@article{
author = "Ćujić, Nada and Savikin, Katarina and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Karanović, Danijela and Grujić-Milanović, Jelica and Jovović, Đurđica and Mihailović-Stanojević, Nevena",
year = "2018",
abstract = "The potential beneficial effects of chronic consumption of dried chokeberry fruit extract (DCE) were evaluated in the present in vivo study. The study concentrates on haemodynamic parameters, lipid profile and oxidative stress in spontaneously hypertensive rats. DCE was characterized by HPLC/DAD method and individual anthocyanins, phenolic acids, and flavonoids were determined. Four week administration of DCE, rich in polyphenols, especially anthocyanins, significantly reduced systolic (P  lt  0.05), pulse pressure (P  lt  0.05) compared to control group, probably via significantly increased diuresis (P  lt  0.05). Significant decrease of plasma and erythrocytes TBARS was also found in the treated group. DCE consumption reduced lipid peroxidation through improving plasma FRAP reducing (P  lt  0.01). The activity of SOD in the treated group had been significantly lower (P  lt  0.01) compared to the control group. These results suggest that DCE could exert beneficial effects on cardiovascular health and has a potential as a nutritional supplement in arterial hypertension.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats",
pages = "339-330",
volume = "44",
doi = "10.1016/j.jff.2018.02.027"
}

Ćujić, N., Savikin, K., Miloradović, Z., Ivanov, M., Vajić, U. J., Karanović, D., Grujić-Milanović, J., Jovović, Đ.,& Mihailović-Stanojević, N.. (2018). Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 44, 330-339.
https://doi.org/10.1016/j.jff.2018.02.027

Ćujić N, Savikin K, Miloradović Z, Ivanov M, Vajić UJ, Karanović D, Grujić-Milanović J, Jovović Đ, Mihailović-Stanojević N. Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats. in Journal of Functional Foods. 2018;44:330-339.
doi:10.1016/j.jff.2018.02.027 .

Ćujić, Nada, Savikin, Katarina, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Karanović, Danijela, Grujić-Milanović, Jelica, Jovović, Đurđica, Mihailović-Stanojević, Nevena, "Characterization of dried chokeberry fruit extract and its chronic effects on blood pressure and oxidative stress in spontaneously hypertensive rats" in Journal of Functional Foods, 44 (2018):330-339,
https://doi.org/10.1016/j.jff.2018.02.027 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Jovanić, Svetlana
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Ilić, Vesna
AU  - Rabasović, Mihailo D.
AU  - Pantelić, Dejan
AU  - Jelenković, Branislav
AU  - Bugarski, Branko
AU  - Krmpot, Aleksandar J.
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/800
AB  - The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.
PB  - Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham
T2  - Journal of Biomedical Optics
T1  - Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy
IS  - 2
VL  - 22
DO  - 10.1117/1.JBO.22.2.026003
ER  - 

@article{
author = "Bukara, Katarina and Jovanić, Svetlana and Drvenica, Ivana and Stančić, Ana and Ilić, Vesna and Rabasović, Mihailo D. and Pantelić, Dejan and Jelenković, Branislav and Bugarski, Branko and Krmpot, Aleksandar J.",
year = "2017",
abstract = "The present study describes utilization of two photon excitation fluorescence (2PE) microscopy for visualization of the hemoglobin in human and porcine erythrocytes and their empty membranes (i.e., ghosts). High-quality, label-and fixation-free visualization of hemoglobin was achieved at excitation wavelength 730 nm by detecting visible autofluorescence. Localization in the suspension and spatial distribution (i.e., mapping) of residual hemoglobin in erythrocyte ghosts has been resolved by 2PE. Prior to the 2PE mapping, the presence of residual hemoglobin in the bulk suspension of erythrocyte ghosts was confirmed by cyanmethemoglobin assay. 2PE analysis revealed that the distribution of hemoglobin in intact erythrocytes follows the cells' shape. Two types of erythrocytes, human and porcine, characterized with discocyte and echinocyte morphology, respectively, showed significant differences in hemoglobin distribution. The 2PE images have revealed that despite an extensive washing out procedure after gradual hypotonic hemolysis, a certain amount of hemoglobin localized on the intracellular side always remains bound to the membrane and cannot be eliminated. The obtained results open the possibility to use 2PE microscopy to examine hemoglobin distribution in erythrocytes and estimate the purity level of erythrocyte ghosts in biotechnological processes.",
publisher = "Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham",
journal = "Journal of Biomedical Optics",
title = "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy",
number = "2",
volume = "22",
doi = "10.1117/1.JBO.22.2.026003"
}

Bukara, K., Jovanić, S., Drvenica, I., Stančić, A., Ilić, V., Rabasović, M. D., Pantelić, D., Jelenković, B., Bugarski, B.,& Krmpot, A. J.. (2017). Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics
Spie-Soc Photo-Optical Instrumentation Engineers, Bellingham., 22(2).
https://doi.org/10.1117/1.JBO.22.2.026003

Bukara K, Jovanić S, Drvenica I, Stančić A, Ilić V, Rabasović MD, Pantelić D, Jelenković B, Bugarski B, Krmpot AJ. Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy. in Journal of Biomedical Optics. 2017;22(2).
doi:10.1117/1.JBO.22.2.026003 .

Bukara, Katarina, Jovanić, Svetlana, Drvenica, Ivana, Stančić, Ana, Ilić, Vesna, Rabasović, Mihailo D., Pantelić, Dejan, Jelenković, Branislav, Bugarski, Branko, Krmpot, Aleksandar J., "Mapping of hemoglobin in erythrocytes and erythrocyte ghosts using two photon excitation fluorescence microscopy" in Journal of Biomedical Optics, 22, no. 2 (2017),
https://doi.org/10.1117/1.JBO.22.2.026003 . .

TY  - JOUR
AU  - Jakovetić-Tanasković, Sonja
AU  - Jokić, Bojan
AU  - Grbavcić, Sanja
AU  - Drvenica, Ivana
AU  - Prlainović, Nevena
AU  - Luković, Nevena
AU  - Knežević-Jugović, Zorica
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/801
AB  - Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Clay Science
T1  - Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants
EP  - 111
SP  - 103
VL  - 135
DO  - 10.1016/j.clay.2016.09.011
ER  - 

@article{
author = "Jakovetić-Tanasković, Sonja and Jokić, Bojan and Grbavcić, Sanja and Drvenica, Ivana and Prlainović, Nevena and Luković, Nevena and Knežević-Jugović, Zorica",
year = "2017",
abstract = "Lipase B from Candida antarctica was immobilized by adsorption onto laboratory prepared metakaolin. This support has great potential for enzyme immobilization since it has good mechanical characteristics and thermal stability and it is easily obtained from naturally abundant raw kaolin, as presented in this paper. Immobilization process was studied as a function of pH, ionic strength and protein concentration in terms of immobilization and activity yield. It was shown that immobilization occurred via the Langmuir model, and that electrostatic forces were major, but not exclusive contributor for the binding process. The optimum conditions were achieved in 10 mM acetic buffer pH 5. Biocatalyst obtained under optimum conditions was further successfully used in the synthesis of lipophilic antioxidants, where conversion yields as high as 100% were achieved.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Clay Science",
title = "Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants",
pages = "111-103",
volume = "135",
doi = "10.1016/j.clay.2016.09.011"
}

Jakovetić-Tanasković, S., Jokić, B., Grbavcić, S., Drvenica, I., Prlainović, N., Luković, N.,& Knežević-Jugović, Z.. (2017). Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants. in Applied Clay Science
Elsevier Science Bv, Amsterdam., 135, 103-111.
https://doi.org/10.1016/j.clay.2016.09.011

Jakovetić-Tanasković S, Jokić B, Grbavcić S, Drvenica I, Prlainović N, Luković N, Knežević-Jugović Z. Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants. in Applied Clay Science. 2017;135:103-111.
doi:10.1016/j.clay.2016.09.011 .

Jakovetić-Tanasković, Sonja, Jokić, Bojan, Grbavcić, Sanja, Drvenica, Ivana, Prlainović, Nevena, Luković, Nevena, Knežević-Jugović, Zorica, "Immobilization of Candida antarctica lipase B on kaolin and its application in synthesis of lipophilic antioxidants" in Applied Clay Science, 135 (2017):103-111,
https://doi.org/10.1016/j.clay.2016.09.011 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valcić, Olivera
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/779
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P lt 0.001 and P lt 0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin lt 10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
EP  - 524
IS  - 4
SP  - 508
VL  - 67
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valcić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P lt 0.001 and P lt 0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin lt 10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
pages = "524-508",
number = "4",
volume = "67",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovačić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valcić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovačić M, Ilić V, Gvozdić D, Savić O, Doković R, Valcić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valcić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .

TY  - JOUR
AU  - Bojkovski, Jovan
AU  - Ilić, Vesna
AU  - Vasić, Ana
AU  - Maletić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Savić, Sara
AU  - Suarez, Manuel
AU  - Zdravković, Nemanja
AU  - Tzika, E. D.
AU  - Nicluae, M.
AU  - Milanov, Dubravka
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/794
AB  - The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p lt 0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p gt 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms
EP  - 354
IS  - 3
SP  - 347
VL  - 68
DO  - 10.12681/jhvms.15488
ER  - 

@article{
author = "Bojkovski, Jovan and Ilić, Vesna and Vasić, Ana and Maletić, Jelena and Vakanjac, Slobodanka and Savić, Sara and Suarez, Manuel and Zdravković, Nemanja and Tzika, E. D. and Nicluae, M. and Milanov, Dubravka",
year = "2017",
abstract = "The aim of the present study was to determine the porcine circovirus type 2 (PCV2) prevalence in boars in 3 farms in Serbia and their possible relation with alterations of reproductive parameters and blood biochemical parameters [total protein, urea, creatinine and aspartate transaminase levels (AST)]. The prevalence of (PCV2) was evaluated by the presence of specific antibodies. An ELISA assay was used for the detection of PCV2 antibodies in 58 boars' blood sera from Serbian commercial farms, among which 48 were from and reared in the Serbian farms, and 10 were imported from different European Union (EU) countries. Anti PCV2 IgM and/or IgG were detected in sera of 51 (87.93%) boars. Based on the type of antibodies (IgM and IgG PCV2 antibodies), it was concluded that chronic PCV2 infection was the predominant type in tested boars. The imported boars did not have an active infection titer. Biochemical tests in blood didn't show significant differences between PCV2 positive and negative boars. The recorded high prevalence of PCV2 antibodies among boars indirectly proved that PCV2 has been wide spread in the examined farms. Significant difference in the frequency of active, recent and chronic PCV2 infection in boars was found among three farms (p lt 0.05). Imported boars did not show significant difference in terms of active, recent or chronic PCV2 infection in comparison with boars deriving from Serbian farms (p gt 0.05). Among boars of three different breeds (Landrace, Duroc and Yorkshire), a significantly higher prevalence of PCV2 antibodies was revealed in Landrace boars (p=0.003). Reproductive parameters of sows that were inseminated with PCV2 positive or negative boars' semen, showed similar farrowing rate, as well as percentage of live and stillborn piglets, thus indicating that the observed chronic PCV2 infection did not affect significantly the breeding capability of boars.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms",
pages = "354-347",
number = "3",
volume = "68",
doi = "10.12681/jhvms.15488"
}

Bojkovski, J., Ilić, V., Vasić, A., Maletić, J., Vakanjac, S., Savić, S., Suarez, M., Zdravković, N., Tzika, E. D., Nicluae, M.,& Milanov, D.. (2017). Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 68(3), 347-354.
https://doi.org/10.12681/jhvms.15488

Bojkovski J, Ilić V, Vasić A, Maletić J, Vakanjac S, Savić S, Suarez M, Zdravković N, Tzika ED, Nicluae M, Milanov D. Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms. in Journal of the Hellenic Veterinary Medical Society. 2017;68(3):347-354.
doi:10.12681/jhvms.15488 .

Bojkovski, Jovan, Ilić, Vesna, Vasić, Ana, Maletić, Jelena, Vakanjac, Slobodanka, Savić, Sara, Suarez, Manuel, Zdravković, Nemanja, Tzika, E. D., Nicluae, M., Milanov, Dubravka, "Investigation of Porcine Circovirus type 2 (PCV2) antibodies in clinically healthy boars from Serbian commercial farms" in Journal of the Hellenic Veterinary Medical Society, 68, no. 3 (2017):347-354,
https://doi.org/10.12681/jhvms.15488 . .

TY  - JOUR
AU  - Bjelica, Jelena
AU  - Jovanović, Ivan B.
AU  - Ilić, Vesna
AU  - Valcić, Olivera
AU  - Kirovski, Danijela
AU  - Gvozdić, Dragan
AU  - Milanović, Svetlana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/760
AB  - Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having "intermediate" concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3  lt = 1.55 nmol/L; T-4  lt = 40 nmol/L), while PTU-groups included euthyroid rats (T-3  lt = 1.70 nmol/L; T-4  lt = 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats
EP  - 355
IS  - 3
SP  - 340
VL  - 67
DO  - 10.1515/acve-2017-0028
ER  - 

@article{
author = "Bjelica, Jelena and Jovanović, Ivan B. and Ilić, Vesna and Valcić, Olivera and Kirovski, Danijela and Gvozdić, Dragan and Milanović, Svetlana",
year = "2017",
abstract = "Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having "intermediate" concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3  lt = 1.55 nmol/L; T-4  lt = 40 nmol/L), while PTU-groups included euthyroid rats (T-3  lt = 1.70 nmol/L; T-4  lt = 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats",
pages = "355-340",
number = "3",
volume = "67",
doi = "10.1515/acve-2017-0028"
}

Bjelica, J., Jovanović, I. B., Ilić, V., Valcić, O., Kirovski, D., Gvozdić, D.,& Milanović, S.. (2017). Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3), 340-355.
https://doi.org/10.1515/acve-2017-0028

Bjelica J, Jovanović IB, Ilić V, Valcić O, Kirovski D, Gvozdić D, Milanović S. Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd. 2017;67(3):340-355.
doi:10.1515/acve-2017-0028 .

Bjelica, Jelena, Jovanović, Ivan B., Ilić, Vesna, Valcić, Olivera, Kirovski, Danijela, Gvozdić, Dragan, Milanović, Svetlana, "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats" in Acta Veterinaria-Beograd, 67, no. 3 (2017):340-355,
https://doi.org/10.1515/acve-2017-0028 . .

TY  - JOUR
AU  - Bukara, Katarina
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Misić, Danijela M.
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš
AU  - Vučetić, Dušan
AU  - Kiekens, Filip
AU  - Bugarski, Branko
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/689
AB  - The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Biotechnology
T1  - Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts
EP  - 22
SP  - 14
VL  - 240
DO  - 10.1016/j.jbiotec.2016.10.017
ER  - 

@article{
author = "Bukara, Katarina and Drvenica, Ivana and Ilić, Vesna and Stančić, Ana and Misić, Danijela M. and Vasić, Borislav Z. and Gajić, Radoš and Vučetić, Dušan and Kiekens, Filip and Bugarski, Branko",
year = "2016",
abstract = "The objective of our study was to develop controlled drug delivery system based on erythrocyte ghosts for amphiphilic compound sodium diclofenac considering the differences between erythrocytes derived from two readily available materials - porcine slaughterhouse and outdated transfusion human blood. Starting erythrocytes, empty erythrocyte ghosts and diclofenac loaded ghosts were compared in terms of the encapsulation efficiency, drug releasing profiles, size distribution, surface charge, conductivity, surface roughness and morphology. The encapsulation of sodium diclofenac was performed by an osmosis based process - gradual hemolysis. During this process sodium diclofenac exerted mild and delayed antihemolytic effect and increased potassium efflux in porcine but not in outdated human erythrocytes. FTIR spectra revealed lack of any membrane lipid disorder and chemical reaction with sodium diclofenac in encapsulated ghosts. Outdated human erythrocyte ghosts with detected nanoscale damages and reduced ability to shrink had encapsulation efficiency of only 8%. On the other hand, porcine erythrocyte ghosts had encapsulation efficiency of 37% and relatively slow drug release rate. More preserved structure and functional properties of porcine erythrocytes related to their superior encapsulation and release performances, define them as more appropriate for the usage in sodium diclofenac encapsulation process.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Biotechnology",
title = "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts",
pages = "22-14",
volume = "240",
doi = "10.1016/j.jbiotec.2016.10.017"
}

Bukara, K., Drvenica, I., Ilić, V., Stančić, A., Misić, D. M., Vasić, B. Z., Gajić, R., Vučetić, D., Kiekens, F.,& Bugarski, B.. (2016). Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology
Elsevier Science Bv, Amsterdam., 240, 14-22.
https://doi.org/10.1016/j.jbiotec.2016.10.017

Bukara K, Drvenica I, Ilić V, Stančić A, Misić DM, Vasić BZ, Gajić R, Vučetić D, Kiekens F, Bugarski B. Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts. in Journal of Biotechnology. 2016;240:14-22.
doi:10.1016/j.jbiotec.2016.10.017 .

Bukara, Katarina, Drvenica, Ivana, Ilić, Vesna, Stančić, Ana, Misić, Danijela M., Vasić, Borislav Z., Gajić, Radoš, Vučetić, Dušan, Kiekens, Filip, Bugarski, Branko, "Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts" in Journal of Biotechnology, 240 (2016):14-22,
https://doi.org/10.1016/j.jbiotec.2016.10.017 . .

TY  - JOUR
AU  - Balanc, Bojana
AU  - Kalušević, Ana
AU  - Drvenica, Ivana
AU  - Coelho, Maria Teresa
AU  - Đorđević, Verica
AU  - Alves, Vitor D.
AU  - Sousa, Isabel
AU  - Moldao-Martins, Margarida
AU  - Rakić, Vesna
AU  - Nedović, Viktor
AU  - Bugarski, Branko
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/720
AB  - Carqueja (Pterospartum tridentatum) is an endemic species and various bioactive compounds have been identified in its aqueous extract. The aim of this study was to protect the natural antioxidants from the aqueous extract of carqueja by encapsulation in Ca-alginate microbeads and Ca-alginate microbeads containing 10% and 20% (w/v) of inulin. The microbeads produced by electrostatic extrusion technique had an average diameter from 625 mu m to 830 mu m depending on the portion of inulin. The sphericity factor of the hydrogel microbeads had values between 0.014 and 0.026, while freeze dried microbeads had irregular shape, especially those with no excipient. The reduction in microbeads size after freeze drying process (expressed as shrinkage factor) ranged from 0.338 (alginate microbeads with 20% (w/v) of inulin) to 0.523 (plain alginate microbeads). The expressed radical scavenging activity against ABTS and DPPH radicals was found to be between 30% and 40% for encapsulated extract, while the fresh extract showed around 47% and 57% of radical scavenging activity for ABTS and DPPH radicals, respectively. The correlation between antioxidant activity and the total phenolic content were found to be positive (in both assay methods, DPPH and ABTS), which indicate that the addition of inulin didn't have influence on antioxidant activity. The presence of inulin reduced stiffness of the hydrogel, and protected bead structure from collapse upon freeze-drying. Alginate-inulin beads are envisaged to be used for delivery of aqueous P. tridentatum extract in functional food products.
PB  - Wiley, Hoboken
T2  - Journal of Food Science
T1  - Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract
EP  - E75
IS  - 1
SP  - E65
VL  - 81
DO  - 10.1111/1750-3841.13167
ER  - 

@article{
author = "Balanc, Bojana and Kalušević, Ana and Drvenica, Ivana and Coelho, Maria Teresa and Đorđević, Verica and Alves, Vitor D. and Sousa, Isabel and Moldao-Martins, Margarida and Rakić, Vesna and Nedović, Viktor and Bugarski, Branko",
year = "2016",
abstract = "Carqueja (Pterospartum tridentatum) is an endemic species and various bioactive compounds have been identified in its aqueous extract. The aim of this study was to protect the natural antioxidants from the aqueous extract of carqueja by encapsulation in Ca-alginate microbeads and Ca-alginate microbeads containing 10% and 20% (w/v) of inulin. The microbeads produced by electrostatic extrusion technique had an average diameter from 625 mu m to 830 mu m depending on the portion of inulin. The sphericity factor of the hydrogel microbeads had values between 0.014 and 0.026, while freeze dried microbeads had irregular shape, especially those with no excipient. The reduction in microbeads size after freeze drying process (expressed as shrinkage factor) ranged from 0.338 (alginate microbeads with 20% (w/v) of inulin) to 0.523 (plain alginate microbeads). The expressed radical scavenging activity against ABTS and DPPH radicals was found to be between 30% and 40% for encapsulated extract, while the fresh extract showed around 47% and 57% of radical scavenging activity for ABTS and DPPH radicals, respectively. The correlation between antioxidant activity and the total phenolic content were found to be positive (in both assay methods, DPPH and ABTS), which indicate that the addition of inulin didn't have influence on antioxidant activity. The presence of inulin reduced stiffness of the hydrogel, and protected bead structure from collapse upon freeze-drying. Alginate-inulin beads are envisaged to be used for delivery of aqueous P. tridentatum extract in functional food products.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Science",
title = "Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract",
pages = "E75-E65",
number = "1",
volume = "81",
doi = "10.1111/1750-3841.13167"
}

Balanc, B., Kalušević, A., Drvenica, I., Coelho, M. T., Đorđević, V., Alves, V. D., Sousa, I., Moldao-Martins, M., Rakić, V., Nedović, V.,& Bugarski, B.. (2016). Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract. in Journal of Food Science
Wiley, Hoboken., 81(1), E65-E75.
https://doi.org/10.1111/1750-3841.13167

Balanc B, Kalušević A, Drvenica I, Coelho MT, Đorđević V, Alves VD, Sousa I, Moldao-Martins M, Rakić V, Nedović V, Bugarski B. Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract. in Journal of Food Science. 2016;81(1):E65-E75.
doi:10.1111/1750-3841.13167 .

Balanc, Bojana, Kalušević, Ana, Drvenica, Ivana, Coelho, Maria Teresa, Đorđević, Verica, Alves, Vitor D., Sousa, Isabel, Moldao-Martins, Margarida, Rakić, Vesna, Nedović, Viktor, Bugarski, Branko, "Calcium-Alginate-Inulin Microbeads as Carriers for Aqueous Carqueja Extract" in Journal of Food Science, 81, no. 1 (2016):E65-E75,
https://doi.org/10.1111/1750-3841.13167 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Bukara, Katarina
AU  - Ilić, Vesna
AU  - Misić, Danijela M.
AU  - Vasić, Borislav Z.
AU  - Gajić, Radoš
AU  - Đorđević Verica B.
AU  - Veljović, Đorđe N.
AU  - Belić, Aleksandar
AU  - Bugarski, Branko
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/736
AB  - The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.
PB  - Wiley, Hoboken
T2  - Biotechnology Progress
T1  - Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate
EP  - 1055
IS  - 4
SP  - 1046
VL  - 32
DO  - 10.1002/btpr.2304
ER  - 

@article{
author = "Drvenica, Ivana and Bukara, Katarina and Ilić, Vesna and Misić, Danijela M. and Vasić, Borislav Z. and Gajić, Radoš and Đorđević Verica B. and Veljović, Đorđe N. and Belić, Aleksandar and Bugarski, Branko",
year = "2016",
abstract = "The present study investigated preparation of bovine and porcine erythrocyte membranes from slaughterhouse blood as bio-derived materials for delivery of dexamethasone-sodium phosphate (DexP). The obtained biomembranes, i.e., ghosts were characterized in vitro in terms of morphological properties, loading parameters, and release behavior. For the last two, an UHPLC/-HESI-MS/MS based analytical procedure for absolute drug identification and quantification was developed. The results revealed that loading of DexP into both type of ghosts was directly proportional to the increase of drug concentration in the incubation medium, while incubation at 37 degrees C had statistically significant effect on loaded amount of DexP (P lt 0.05). The encapsulation efficiency was about fivefold higher in porcine compared to bovine ghosts. Insight into ghosts' surface morphology by field emission-scanning electron microscopy and atomic force microscopy confirmed that besides inevitable effects of osmosis, DexP inclusion itself had no observable additional effect on the morphology of the ghosts carriers. DexP release profiles were dependent on erythrocyte ghost type and amount of residual hemoglobin. However, sustained DexP release was achieved and shown over 3 days from porcine ghosts and 5 days from bovine erythrocyte ghosts.",
publisher = "Wiley, Hoboken",
journal = "Biotechnology Progress",
title = "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate",
pages = "1055-1046",
number = "4",
volume = "32",
doi = "10.1002/btpr.2304"
}

Drvenica, I., Bukara, K., Ilić, V., Misić, D. M., Vasić, B. Z., Gajić, R., Đorđević Verica B., Veljović, Đ. N., Belić, A.,& Bugarski, B.. (2016). Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress
Wiley, Hoboken., 32(4), 1046-1055.
https://doi.org/10.1002/btpr.2304

Drvenica I, Bukara K, Ilić V, Misić DM, Vasić BZ, Gajić R, Đorđević Verica B., Veljović ĐN, Belić A, Bugarski B. Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate. in Biotechnology Progress. 2016;32(4):1046-1055.
doi:10.1002/btpr.2304 .

Drvenica, Ivana, Bukara, Katarina, Ilić, Vesna, Misić, Danijela M., Vasić, Borislav Z., Gajić, Radoš, Đorđević Verica B., Veljović, Đorđe N., Belić, Aleksandar, Bugarski, Branko, "Biomembranes from slaughterhouse blood erythrocytes as prolonged release systems for dexamethasone sodium phosphate" in Biotechnology Progress, 32, no. 4 (2016):1046-1055,
https://doi.org/10.1002/btpr.2304 . .

TY  - JOUR
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Bugarski, Branko
AU  - Jovović, Đurđica
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Komes, Draženka
AU  - Grujić-Milanović, Jelica
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/694
AB  - High blood pressure is the most powerful contributor to the cardiovascular morbidity and mortality, and inverse correlation between consumption of polyphenol-rich foods or beverages and incidence of cardiovascular diseases gains more importance. Reactive oxygen species plays an important role in the development of hypertension. We found that wild thyme (a spice plant, rich in polyphenolic compounds) induced a significant decrease of blood pressure and vascular resistance in hypertensive rats. The inverse correlation between vascular resistance and plasma heme oxygenase-1 suggests that endogenous vasodilator carbon monoxide generated by heme oxidation could account for this normalization of blood pressure. Next product of heme oxidation, bilirubin (a chain-breaking antioxidant that acts as a lipid peroxyl radical scavenger), becomes significantly increased after wild thyme treatment and induces the reduction of plasma lipid peroxidation in hypertensive, but not in normotensive rats. The obtained results promote wild thyme as useful supplement for cardiovascular interventions.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine & Cellular Longevity
T1  - Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress
VL  - 2016
DO  - 10.1155/2016/1458793
ER  - 

@article{
author = "Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Bugarski, Branko and Jovović, Đurđica and Karanović, Danijela and Vajić, Una Jovana and Komes, Draženka and Grujić-Milanović, Jelica",
year = "2016",
abstract = "High blood pressure is the most powerful contributor to the cardiovascular morbidity and mortality, and inverse correlation between consumption of polyphenol-rich foods or beverages and incidence of cardiovascular diseases gains more importance. Reactive oxygen species plays an important role in the development of hypertension. We found that wild thyme (a spice plant, rich in polyphenolic compounds) induced a significant decrease of blood pressure and vascular resistance in hypertensive rats. The inverse correlation between vascular resistance and plasma heme oxygenase-1 suggests that endogenous vasodilator carbon monoxide generated by heme oxidation could account for this normalization of blood pressure. Next product of heme oxidation, bilirubin (a chain-breaking antioxidant that acts as a lipid peroxyl radical scavenger), becomes significantly increased after wild thyme treatment and induces the reduction of plasma lipid peroxidation in hypertensive, but not in normotensive rats. The obtained results promote wild thyme as useful supplement for cardiovascular interventions.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine & Cellular Longevity",
title = "Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress",
volume = "2016",
doi = "10.1155/2016/1458793"
}

Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Bugarski, B., Jovović, Đ., Karanović, D., Vajić, U. J., Komes, D.,& Grujić-Milanović, J.. (2016). Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress. in Oxidative Medicine & Cellular Longevity
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/1458793

Mihailović-Stanojević N, Miloradović Z, Ivanov M, Bugarski B, Jovović Đ, Karanović D, Vajić UJ, Komes D, Grujić-Milanović J. Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress. in Oxidative Medicine & Cellular Longevity. 2016;2016.
doi:10.1155/2016/1458793 .

Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Bugarski, Branko, Jovović, Đurđica, Karanović, Danijela, Vajić, Una Jovana, Komes, Draženka, Grujić-Milanović, Jelica, "Upregulation of Heme Oxygenase-1 in Response to Wild Thyme Treatment Protects against Hypertension and Oxidative Stress" in Oxidative Medicine & Cellular Longevity, 2016 (2016),
https://doi.org/10.1155/2016/1458793 . .

TY  - CHAP
AU  - Nedović, Viktor
AU  - Bugarski, Branko
AU  - Mantzouridou, F.
AU  - Paraskevopoulou, A.
AU  - Naziri, E.
AU  - Koupantsis, T.
AU  - Trifković, Kata
AU  - Drvenica, Ivana
AU  - Balanc, Bojana
AU  - Ðordević, V.
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/607
AB  - Encapsulation is a well-established process that allows formulation of a wide range of active agents within a defined carrier material using various techniques. This chapter describes the general concept and benefits of microbial and non-microbial active food ingredient encapsulation, existing encapsulation technologies and examples of the use of loaded capsules for the protection and enhanced delivery of these constituents in food products. We describe existing knowledge of the most important characteristics of food-specific materials, capsule morphologies and methods that are applied or that may be tailored to encapsulation of active ingredients, with emphasis on microbial cells, bioactive compounds and flavours, for use in food industry. Improvements made by encapsulated cell technology in fermentation processes and in the microbial production of high-value food ingredients are reviewed. Current trends and future perspectives are also discussed.
T2  - Advances in Food Biotechnology
T1  - Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture
EP  - 680
SP  - 635
DO  - 10.1002/9781118864463.ch39
ER  - 

@inbook{
author = "Nedović, Viktor and Bugarski, Branko and Mantzouridou, F. and Paraskevopoulou, A. and Naziri, E. and Koupantsis, T. and Trifković, Kata and Drvenica, Ivana and Balanc, Bojana and Ðordević, V.",
year = "2015",
abstract = "Encapsulation is a well-established process that allows formulation of a wide range of active agents within a defined carrier material using various techniques. This chapter describes the general concept and benefits of microbial and non-microbial active food ingredient encapsulation, existing encapsulation technologies and examples of the use of loaded capsules for the protection and enhanced delivery of these constituents in food products. We describe existing knowledge of the most important characteristics of food-specific materials, capsule morphologies and methods that are applied or that may be tailored to encapsulation of active ingredients, with emphasis on microbial cells, bioactive compounds and flavours, for use in food industry. Improvements made by encapsulated cell technology in fermentation processes and in the microbial production of high-value food ingredients are reviewed. Current trends and future perspectives are also discussed.",
journal = "Advances in Food Biotechnology",
booktitle = "Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture",
pages = "680-635",
doi = "10.1002/9781118864463.ch39"
}

Nedović, V., Bugarski, B., Mantzouridou, F., Paraskevopoulou, A., Naziri, E., Koupantsis, T., Trifković, K., Drvenica, I., Balanc, B.,& Ðordević, V.. (2015). Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture. in Advances in Food Biotechnology, 635-680.
https://doi.org/10.1002/9781118864463.ch39

Nedović V, Bugarski B, Mantzouridou F, Paraskevopoulou A, Naziri E, Koupantsis T, Trifković K, Drvenica I, Balanc B, Ðordević V. Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture. in Advances in Food Biotechnology. 2015;:635-680.
doi:10.1002/9781118864463.ch39 .

Nedović, Viktor, Bugarski, Branko, Mantzouridou, F., Paraskevopoulou, A., Naziri, E., Koupantsis, T., Trifković, Kata, Drvenica, Ivana, Balanc, Bojana, Ðordević, V., "Recent advances in and applications of encapsulated microbial and non-microbial active agents in food and beverage manufacture" in Advances in Food Biotechnology (2015):635-680,
https://doi.org/10.1002/9781118864463.ch39 . .

TY  - THES
AU  - Kostić, Ivana T.
PY  - 2015
UR  - http://nardus.mpn.gov.rs/handle/123456789/4566
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2413
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10420/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=47631375
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1102
AB  - In this doctoral dissertation, а process for production of empty erythrocyte membranes („ghosts“) originating from bovine and porcine wasted slaughterhouse blood was developed. In addition, with an aim of designing prolonged drug delivery vehicles, ghost-based drug delivery systems were formulated and in vitro characterized. In order to design the process by which hemoglobin would be released from erythrocytes, leaving preserved cell membranes, the first step was to define specific osmotic properties of bovine and porcine erythrocytes derived from slaughterhouse blood. The results showed that porcine erythrocytes were more osmotically susceptible compared to bovine ones, but neither of the two examined types of erythrocytes was homogeneous regarding their osmotic properties. This heterogeneity was detected among erythrocytes from different samples of one species, as well as among erythrocytes in one sample. The hypotonic sodiumphosphate/ NaCl buffer of 35 mM concentration circulating with a flow rate of 300 ml/h during 30 min was optimal for conducting gradual hypotonic hemolysis. Under these conditions the process of gradual hemolysis resulted with a high hemolysis extent (>90%). Biochemical analysis revealed that protein and cholesterol content of isolated ghosts were nearly the same as in intact erythrocytes. Compared to starting erythrocytes, average diameter of the ghosts from both type of erythrocytes decreased for only 10%. These results indicate that isolated membranes have possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, and this fact is of significance for drug diffusion and partition coefficients during encapsulation procedure. In the next step, the gradual hypotonic hemolysis was evaluated as an encapsulation procedure for a model drug into the ghosts which were obtained in the previous experimental stage. A synthetic glucocorticoid, widely used antiinflammatory drug, dexamethasone sodium phosphate (DexP), was selected as a model compound. The processing parameters of DexP encapsulation into the ghosts-temperature, initial drug concentration, and cross-linker addition- were optimized...
AB  - U ovoj doktorskoj disertaciji razvijen je proces za izolovanje goveđih i svinjskih eritrocitnih membrana („duhova”) iz otpadne klanične krvi. Pored toga, razvijeni su i detaljno in vitro okarakterisani sistemi za produženo oslobađanje aktivnih supstanci na bazi dobijenih eritrocitnih membrana. U cilju projektovanja procesa kojim bi se dobile goveđe i svinjske eritrocitne membrane koje su strukturno očuvane, a poreklom iz otpadne klanične krvi, definisane su specifične osmotske karakteristike ovih eritrocita. Pokazano je da su svinjski eritroci osmotski osetljiviji i da eritrociti nijedne od ispitivanih vrsta nisu osmotski homogeni. Ova heterogenost je detektovana između eritrocita različitih uzoraka iste vrste, ali i eritrocita jednog uzorka. Kao optimalni pufer za izvođenje hemolize goveđih i svinjskih eritrocita određen je 35 mM natrijum fosfatni/NaCl pufer čijom primenom se u procesu gradualne hipotonične hemolize, pri protoku pufera od 300 ml/h, tokom 30 min, oslobađa >90% hemoglobina iz velike zapremine (100 ml) obe vrste eritrocita. Biohemijska analiza dobijenih membrana eritrocita je pokazala da se sadržaj proteina i holesterola nije bitno razlikovao u odnosu na membrane intaktnih eritrocita. Takođe, prečnik eritrocitnih membrana obe vrste bio je za samo 10% manji od intaktnih eritrocita, a raspodela veličine uniformna. Ovi rezultati su pokazali da duhovi klaničnih eritrocita obe vrste životinja imaju sposobnost da „imitiraju" hemijsko i strukturno anizotropno okruženje ćelijskih membrana in vivo, što je od značaja za difuziju i particioni koeficijet aktivnih supstanci pri njihovoj inkapsulaciji. U sledećoj fazi istraživanja ispitana je mogućnosti primene procesa gradualne hipotonične hemolize za inkapsulaciju model supstance, sintetskog glukokortikoida i najšire korišćenog antiinflamatornog leka, deksametazon-natrijum-fosfata (DexP)...
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Preserved erythrocyte membranes produced from slaughterhouse blood as systems for prolonged delivery of active substances
T1  - Procesno očuvane membrane eritrocita dobijene iz klanične krvi kao sistemi za produženo oslobađanje aktivnih supstanci
UR  - https://hdl.handle.net/21.15107/rcub_nardus_4566
ER  - 

@phdthesis{
author = "Kostić, Ivana T.",
year = "2015",
abstract = "In this doctoral dissertation, а process for production of empty erythrocyte membranes („ghosts“) originating from bovine and porcine wasted slaughterhouse blood was developed. In addition, with an aim of designing prolonged drug delivery vehicles, ghost-based drug delivery systems were formulated and in vitro characterized. In order to design the process by which hemoglobin would be released from erythrocytes, leaving preserved cell membranes, the first step was to define specific osmotic properties of bovine and porcine erythrocytes derived from slaughterhouse blood. The results showed that porcine erythrocytes were more osmotically susceptible compared to bovine ones, but neither of the two examined types of erythrocytes was homogeneous regarding their osmotic properties. This heterogeneity was detected among erythrocytes from different samples of one species, as well as among erythrocytes in one sample. The hypotonic sodiumphosphate/ NaCl buffer of 35 mM concentration circulating with a flow rate of 300 ml/h during 30 min was optimal for conducting gradual hypotonic hemolysis. Under these conditions the process of gradual hemolysis resulted with a high hemolysis extent (>90%). Biochemical analysis revealed that protein and cholesterol content of isolated ghosts were nearly the same as in intact erythrocytes. Compared to starting erythrocytes, average diameter of the ghosts from both type of erythrocytes decreased for only 10%. These results indicate that isolated membranes have possibility to mimic the chemical and structural anisotropic environment of in vivo cell membranes, and this fact is of significance for drug diffusion and partition coefficients during encapsulation procedure. In the next step, the gradual hypotonic hemolysis was evaluated as an encapsulation procedure for a model drug into the ghosts which were obtained in the previous experimental stage. A synthetic glucocorticoid, widely used antiinflammatory drug, dexamethasone sodium phosphate (DexP), was selected as a model compound. The processing parameters of DexP encapsulation into the ghosts-temperature, initial drug concentration, and cross-linker addition- were optimized..., U ovoj doktorskoj disertaciji razvijen je proces za izolovanje goveđih i svinjskih eritrocitnih membrana („duhova”) iz otpadne klanične krvi. Pored toga, razvijeni su i detaljno in vitro okarakterisani sistemi za produženo oslobađanje aktivnih supstanci na bazi dobijenih eritrocitnih membrana. U cilju projektovanja procesa kojim bi se dobile goveđe i svinjske eritrocitne membrane koje su strukturno očuvane, a poreklom iz otpadne klanične krvi, definisane su specifične osmotske karakteristike ovih eritrocita. Pokazano je da su svinjski eritroci osmotski osetljiviji i da eritrociti nijedne od ispitivanih vrsta nisu osmotski homogeni. Ova heterogenost je detektovana između eritrocita različitih uzoraka iste vrste, ali i eritrocita jednog uzorka. Kao optimalni pufer za izvođenje hemolize goveđih i svinjskih eritrocita određen je 35 mM natrijum fosfatni/NaCl pufer čijom primenom se u procesu gradualne hipotonične hemolize, pri protoku pufera od 300 ml/h, tokom 30 min, oslobađa >90% hemoglobina iz velike zapremine (100 ml) obe vrste eritrocita. Biohemijska analiza dobijenih membrana eritrocita je pokazala da se sadržaj proteina i holesterola nije bitno razlikovao u odnosu na membrane intaktnih eritrocita. Takođe, prečnik eritrocitnih membrana obe vrste bio je za samo 10% manji od intaktnih eritrocita, a raspodela veličine uniformna. Ovi rezultati su pokazali da duhovi klaničnih eritrocita obe vrste životinja imaju sposobnost da „imitiraju" hemijsko i strukturno anizotropno okruženje ćelijskih membrana in vivo, što je od značaja za difuziju i particioni koeficijet aktivnih supstanci pri njihovoj inkapsulaciji. U sledećoj fazi istraživanja ispitana je mogućnosti primene procesa gradualne hipotonične hemolize za inkapsulaciju model supstance, sintetskog glukokortikoida i najšire korišćenog antiinflamatornog leka, deksametazon-natrijum-fosfata (DexP)...",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Preserved erythrocyte membranes produced from slaughterhouse blood as systems for prolonged delivery of active substances, Procesno očuvane membrane eritrocita dobijene iz klanične krvi kao sistemi za produženo oslobađanje aktivnih supstanci",
url = "https://hdl.handle.net/21.15107/rcub_nardus_4566"
}

Kostić, I. T.. (2015). Preserved erythrocyte membranes produced from slaughterhouse blood as systems for prolonged delivery of active substances. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_4566

Kostić IT. Preserved erythrocyte membranes produced from slaughterhouse blood as systems for prolonged delivery of active substances. 2015;.
https://hdl.handle.net/21.15107/rcub_nardus_4566 .

Kostić, Ivana T., "Preserved erythrocyte membranes produced from slaughterhouse blood as systems for prolonged delivery of active substances" (2015),
https://hdl.handle.net/21.15107/rcub_nardus_4566 .

TY  - JOUR
AU  - Kostić, Ivana
AU  - Ilić, Vesna
AU  - Bukara, Katarina
AU  - Mojsilović, Slavko
AU  - Đurić, Zorka Ž.
AU  - Draškovič, Petra
AU  - Bugarski, Branko
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/655
AB  - Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected.
AB  - Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery
T1  - Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova
EP  - 76
IS  - 1
SP  - 67
VL  - 69
DO  - 10.2298/HEMIND140124021K
ER  - 

@article{
author = "Kostić, Ivana and Ilić, Vesna and Bukara, Katarina and Mojsilović, Slavko and Đurić, Zorka Ž. and Draškovič, Petra and Bugarski, Branko",
year = "2015",
abstract = "Despite the fact that the methods based on the osmotic properties of the cells are the most widely used for loading of drugs in human and animal erythrocytes, data related to the osmotic properties of erythrocytes derived from animal blood are scarce. This work was performed with an aim to investigate the possibility of use the flow cytometry as a tool for determination the osmotic behaviour of porcine and bovine erythrocytes, and thus facilitates the engineering of erythrocytes from animal blood to be drug carriers. The method of flow cytometry successfully provided the information about bovine and porcine erythrocyte osmotic fragility, and made the initial steps in assessment of erythrocyte shape in a large number of erythrocytes. Although this method is not able to confirm the swelling of porcine erythrocytes, it indicated the differences in porcine erythrocytes that had basic hematological parameters inside and outside the reference values. In order to apply/use the porcine and bovine erythrocytes as drug carriers, the method of flow cytometry, confirming the presence of osmotically different fractions of red blood cells, indicated that various amounts of the encapsulated drug in porcine and bovine erythrocytes can be expected., Uprkos činjenici da su metode koje se baziraju na osmotskim osobinama ćelija najčešće korišćene metode za inkapsulaciju lekova u humane i životinjske eritrocite, podaci o osmotskim osobinama eritrocita životinjskog porekla su vrlo oskudni. Cilj ovog rada bio je ispitivanje mogućnosti korišćenja metode protočne citometrije za određivanje osmotskih osobina svinjskih i goveđih eritrocita, čime bi se olakšao inženjering pomenutih životinjskih eritrocita za otpuštanje lekova. Metodom protočne citometrije uspešno su dobijene informacije o osmotskoj fragilnosti svinjskih i goveđih eritrocita i načinjeni su početni koraci u proceni oblika velikog broja eritrocita. Iako ova metoda nije uspela da potvrdi bubrenje svinjskih eritrocita, ukazala je na razliku u uzorcima svinjskih eritrocita koji su imali osnovne hematološke parametre izvan i unutar referentnih vrednosti. U cilju primene svinjskih i goveđih eritrocita kao nosača lekova, metoda protočne citometrije je, potvrdivši prisustvo osmotski različitih frakcija eritrocita, ukazala na to da se različite količine inkapsuliranog leka u pojedinačnim, kako svinjskim, tako i goveđim eritrocitima mogu očekivati.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery, Određivanje osmotskih osobina životinjskih eritrocita protočnom citometrijom u cilju njihovog inženjeringa kao nosača lekova",
pages = "76-67",
number = "1",
volume = "69",
doi = "10.2298/HEMIND140124021K"
}

Kostić, I., Ilić, V., Bukara, K., Mojsilović, S., Đurić, Z. Ž., Draškovič, P.,& Bugarski, B.. (2015). Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 69(1), 67-76.
https://doi.org/10.2298/HEMIND140124021K

Kostić I, Ilić V, Bukara K, Mojsilović S, Đurić ZŽ, Draškovič P, Bugarski B. Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery. in Hemijska industrija. 2015;69(1):67-76.
doi:10.2298/HEMIND140124021K .

Kostić, Ivana, Ilić, Vesna, Bukara, Katarina, Mojsilović, Slavko, Đurić, Zorka Ž., Draškovič, Petra, Bugarski, Branko, "Flow cytometric determination of osmotic behaviour of animal erythrocytes toward their engineering for drug delivery" in Hemijska industrija, 69, no. 1 (2015):67-76,
https://doi.org/10.2298/HEMIND140124021K . .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Živković, Jelena
AU  - Savikin, Katarina
AU  - Gođevac, Dejan
AU  - Miloradović, Zoran
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/669
AB  - A full three level factorial design was implemented for optimization of extraction parameters in order to maximize total phenolic (TP) yield from stinging nettle leaf. Factors considered were percentage of methanol in solvent (X-1 : 50-100% methanol) and extraction time (X-2: 30-90 min), and maceration was used as extraction method. A second-order polynomial model was applied for fitting experimental data and predicting the response, and this mathematical model produced a satisfactory fit (R-2 = 0.993,p lt 0.01). The optimal extraction conditions were 54% aqueous methanol and 38 min extraction time, while maximal theoretical TP yield was 8.9 mg GAE/g DW. Solvent composition significantly affected extraction efficiency causing decrease of TP yield with increase of methanol percentage. On the other hand, extraction time did not influence significantly efficiency of extraction. Using LC/MS and HPLC analysis we detected and quantified three most abundant phenolic compounds: 2-O-caffeoyl malic acid, chlorogenic acid, and rutin. Comparison between maceration and ultrasound-assisted extraction (UAE) obtained extracts based on TP content as well as antiradical activity and HPLC results, showed that UAE have a better extraction capability affecting yield and time of extraction. Of all tested extracts, 54% aqueous methanolic extracts obtained with UAE and 38 min extraction time had the highest TP content.
PB  - Elsevier, Amsterdam
T2  - Industrial Crops & Products
T1  - Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology
EP  - 917
SP  - 912
VL  - 74
DO  - 10.1016/j.indcrop.2015.06.032
ER  - 

@article{
author = "Vajić, Una Jovana and Grujić-Milanović, Jelica and Živković, Jelena and Savikin, Katarina and Gođevac, Dejan and Miloradović, Zoran and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2015",
abstract = "A full three level factorial design was implemented for optimization of extraction parameters in order to maximize total phenolic (TP) yield from stinging nettle leaf. Factors considered were percentage of methanol in solvent (X-1 : 50-100% methanol) and extraction time (X-2: 30-90 min), and maceration was used as extraction method. A second-order polynomial model was applied for fitting experimental data and predicting the response, and this mathematical model produced a satisfactory fit (R-2 = 0.993,p lt 0.01). The optimal extraction conditions were 54% aqueous methanol and 38 min extraction time, while maximal theoretical TP yield was 8.9 mg GAE/g DW. Solvent composition significantly affected extraction efficiency causing decrease of TP yield with increase of methanol percentage. On the other hand, extraction time did not influence significantly efficiency of extraction. Using LC/MS and HPLC analysis we detected and quantified three most abundant phenolic compounds: 2-O-caffeoyl malic acid, chlorogenic acid, and rutin. Comparison between maceration and ultrasound-assisted extraction (UAE) obtained extracts based on TP content as well as antiradical activity and HPLC results, showed that UAE have a better extraction capability affecting yield and time of extraction. Of all tested extracts, 54% aqueous methanolic extracts obtained with UAE and 38 min extraction time had the highest TP content.",
publisher = "Elsevier, Amsterdam",
journal = "Industrial Crops & Products",
title = "Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology",
pages = "917-912",
volume = "74",
doi = "10.1016/j.indcrop.2015.06.032"
}

Vajić, U. J., Grujić-Milanović, J., Živković, J., Savikin, K., Gođevac, D., Miloradović, Z., Bugarski, B.,& Mihailović-Stanojević, N.. (2015). Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. in Industrial Crops & Products
Elsevier, Amsterdam., 74, 912-917.
https://doi.org/10.1016/j.indcrop.2015.06.032

Vajić UJ, Grujić-Milanović J, Živković J, Savikin K, Gođevac D, Miloradović Z, Bugarski B, Mihailović-Stanojević N. Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology. in Industrial Crops & Products. 2015;74:912-917.
doi:10.1016/j.indcrop.2015.06.032 .

Vajić, Una Jovana, Grujić-Milanović, Jelica, Živković, Jelena, Savikin, Katarina, Gođevac, Dejan, Miloradović, Zoran, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of extraction of stinging nettle leaf phenolic compounds using response surface methodology" in Industrial Crops & Products, 74 (2015):912-917,
https://doi.org/10.1016/j.indcrop.2015.06.032 . .

TY  - JOUR
AU  - Dordević, Verica
AU  - Balanc, Bojana
AU  - Belscak-Cvitanović, Ana
AU  - Lević, Steva
AU  - Trifković, Kata
AU  - Kalušević, Ana
AU  - Kostić, Ivana
AU  - Komes, Draženka
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/620
AB  - The food industry expects increasingly complex properties (such as delayed release, stability, thermal protection, and suitable sensorial profile) from food ingredients, which often would not be able to be achieved without microencapsulation. This paper presents the state of the art in encapsulation technology for delivery of bioactive compounds to food. It reviews common encapsulation technologies (emphasizing their advantages and limitations) versus novel, interesting approaches in emerging technologies. This review includes a presentation of benefits resulting from the use of microencapsulated ingredients in the food industry; these benefits are going to be illustrated via few case studies bringing innovative processing. Spray drying has been used for more than 60 years to protect flavor oils against degradation/oxidation/evaporation, but melt dispersion technique has been used lately to effectively stabilize an aroma compound. Microgels produced by extrusion and emulsification techniques are considered for delivering synergistic antioxidant effects of plant extract polyphenols, their off-taste masking, and improved handling. Apart from microgels, microemulsions (produced by microfluidization or micelle formation techniques) are taken into account for entrapment of extracts containing polyphenols and essential oils. Innovative and interesting coacervation processes are depicted here as they facilitate the commercialization of coacervated food ingredients. Liposomes are gaining increasing attention in the food sector as they can provide good stability even in a water surrounding and also targeted delivery. The new scalable manufacturing protocols for the production of liposomes evolved in recent years (e.g., proliposome method) are presented here. Fluidized bed technology has been offering a versatile possibility to produce encapsulates which should release ingredients at the right place and the right time. Complex systems such as lipids in hydrogels are newly developed structures for controlled release of bioactive compounds. Finally, the effect encapsulates have when incorporated into real food products will be discussed, in particular with regard to the production of innovative functional food products. As an example, textural, sensorial, and physical quality assessment of chocolates enriched with encapsulated polyphenolic antioxidants from yarrow (Achillea millefolium L.) will be reviewed.
PB  - Springer, New York
T2  - Food Engineering Reviews
T1  - Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds
EP  - 490
IS  - 4
SP  - 452
VL  - 7
DO  - 10.1007/s12393-014-9106-7
ER  - 

@article{
author = "Dordević, Verica and Balanc, Bojana and Belscak-Cvitanović, Ana and Lević, Steva and Trifković, Kata and Kalušević, Ana and Kostić, Ivana and Komes, Draženka and Bugarski, Branko and Nedović, Viktor",
year = "2015",
abstract = "The food industry expects increasingly complex properties (such as delayed release, stability, thermal protection, and suitable sensorial profile) from food ingredients, which often would not be able to be achieved without microencapsulation. This paper presents the state of the art in encapsulation technology for delivery of bioactive compounds to food. It reviews common encapsulation technologies (emphasizing their advantages and limitations) versus novel, interesting approaches in emerging technologies. This review includes a presentation of benefits resulting from the use of microencapsulated ingredients in the food industry; these benefits are going to be illustrated via few case studies bringing innovative processing. Spray drying has been used for more than 60 years to protect flavor oils against degradation/oxidation/evaporation, but melt dispersion technique has been used lately to effectively stabilize an aroma compound. Microgels produced by extrusion and emulsification techniques are considered for delivering synergistic antioxidant effects of plant extract polyphenols, their off-taste masking, and improved handling. Apart from microgels, microemulsions (produced by microfluidization or micelle formation techniques) are taken into account for entrapment of extracts containing polyphenols and essential oils. Innovative and interesting coacervation processes are depicted here as they facilitate the commercialization of coacervated food ingredients. Liposomes are gaining increasing attention in the food sector as they can provide good stability even in a water surrounding and also targeted delivery. The new scalable manufacturing protocols for the production of liposomes evolved in recent years (e.g., proliposome method) are presented here. Fluidized bed technology has been offering a versatile possibility to produce encapsulates which should release ingredients at the right place and the right time. Complex systems such as lipids in hydrogels are newly developed structures for controlled release of bioactive compounds. Finally, the effect encapsulates have when incorporated into real food products will be discussed, in particular with regard to the production of innovative functional food products. As an example, textural, sensorial, and physical quality assessment of chocolates enriched with encapsulated polyphenolic antioxidants from yarrow (Achillea millefolium L.) will be reviewed.",
publisher = "Springer, New York",
journal = "Food Engineering Reviews",
title = "Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds",
pages = "490-452",
number = "4",
volume = "7",
doi = "10.1007/s12393-014-9106-7"
}

Dordević, V., Balanc, B., Belscak-Cvitanović, A., Lević, S., Trifković, K., Kalušević, A., Kostić, I., Komes, D., Bugarski, B.,& Nedović, V.. (2015). Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds. in Food Engineering Reviews
Springer, New York., 7(4), 452-490.
https://doi.org/10.1007/s12393-014-9106-7

Dordević V, Balanc B, Belscak-Cvitanović A, Lević S, Trifković K, Kalušević A, Kostić I, Komes D, Bugarski B, Nedović V. Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds. in Food Engineering Reviews. 2015;7(4):452-490.
doi:10.1007/s12393-014-9106-7 .

Dordević, Verica, Balanc, Bojana, Belscak-Cvitanović, Ana, Lević, Steva, Trifković, Kata, Kalušević, Ana, Kostić, Ivana, Komes, Draženka, Bugarski, Branko, Nedović, Viktor, "Trends in Encapsulation Technologies for Delivery of Food Bioactive Compounds" in Food Engineering Reviews, 7, no. 4 (2015):452-490,
https://doi.org/10.1007/s12393-014-9106-7 . .