TY  - JOUR
AU  - Stojić, Milica
AU  - Ilić, Vesna
AU  - Kovačić, Marijana
AU  - Gvozdić, Dragan
AU  - Stajković, Silvana
AU  - Vejnović, Branislav
AU  - Savić, Olivera
AU  - Fratrić, Natalija
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1017
AB  - This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows
EP  - 435
IS  - 4
SP  - 429
VL  - 87
DO  - 10.1017/S0022029920001077
ER  - 

@article{
author = "Stojić, Milica and Ilić, Vesna and Kovačić, Marijana and Gvozdić, Dragan and Stajković, Silvana and Vejnović, Branislav and Savić, Olivera and Fratrić, Natalija",
year = "2020",
abstract = "This research paper addresses the hypothesis that an oral supplementation with organically modified clinoptilolite will improve colostrum quality in primiparous dairy cows whilst having no adverse effects on the cows' health. A total of 36 pregnant Holstein primiparous dairy cattle were randomly assigned to receive daily oral drenching, two hours following morning feeding, with 1 l of water containing either 0 g/l (n = 16) or 150 g/l (n = 20) of clinoptilolite. Treatment lasted from 24 +/- 4 d prior to expected parturition until two days postpartum (pp). Colostrum was collected at 2 to 3 h, 12, 24 and 36 h pp and blood samples were collected at 24 +/- 4 and 4 +/- 2 d prior to parturition and 1, 2 and 7 d pp. Overall mean dry matter, fat and total protein percentage as well as IgG concentration and mass were significantly greater in colostrum collected from cattle drenched with clinoptilolite (total protein increased by 15% and IgG concentration and mass by 21 and 38% respectively at first sampling and further at second sampling). Total gamma globulin and most other blood serum biochemistry parameters did not differ between cattle treated and not treated with clinoptilolite, the only exception being the fast anionic gamma globulin fraction that was 17% greater at 4 +/- 2 d prior to parturition and 10% lower on the 1st day pp in treated cattle. These results showed that organically modified oral clinoptilolite supplementation at 150 g/d significantly increases the IgG concentration in colostrum and has no adverse effects on the energy status, protein, lipid, and mineral metabolism in primiparous dairy cattle during prepartum period.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows",
pages = "435-429",
number = "4",
volume = "87",
doi = "10.1017/S0022029920001077"
}

Stojić, M., Ilić, V., Kovačić, M., Gvozdić, D., Stajković, S., Vejnović, B., Savić, O.,& Fratrić, N.. (2020). Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research
Cambridge Univ Press, New York., 87(4), 429-435.
https://doi.org/10.1017/S0022029920001077

Stojić M, Ilić V, Kovačić M, Gvozdić D, Stajković S, Vejnović B, Savić O, Fratrić N. Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows. in Journal of Dairy Research. 2020;87(4):429-435.
doi:10.1017/S0022029920001077 .

Stojić, Milica, Ilić, Vesna, Kovačić, Marijana, Gvozdić, Dragan, Stajković, Silvana, Vejnović, Branislav, Savić, Olivera, Fratrić, Natalija, "Effects of oral supplementation with organically modified clinoptilolite during prepartum period on colostrum quality in primiparous dairy cows" in Journal of Dairy Research, 87, no. 4 (2020):429-435,
https://doi.org/10.1017/S0022029920001077 . .

TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valcić, Olivera
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/779
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P lt 0.001 and P lt 0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin lt 10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
EP  - 524
IS  - 4
SP  - 508
VL  - 67
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovačić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valcić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P lt 0.001 and P lt 0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin lt 10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
pages = "524-508",
number = "4",
volume = "67",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovačić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valcić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovačić M, Ilić V, Gvozdić D, Savić O, Doković R, Valcić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovačić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valcić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .

TY  - JOUR
AU  - Bjelica, Jelena
AU  - Jovanović, Ivan B.
AU  - Ilić, Vesna
AU  - Valcić, Olivera
AU  - Kirovski, Danijela
AU  - Gvozdić, Dragan
AU  - Milanović, Svetlana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/760
AB  - Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having "intermediate" concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3  lt = 1.55 nmol/L; T-4  lt = 40 nmol/L), while PTU-groups included euthyroid rats (T-3  lt = 1.70 nmol/L; T-4  lt = 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats
EP  - 355
IS  - 3
SP  - 340
VL  - 67
DO  - 10.1515/acve-2017-0028
ER  - 

@article{
author = "Bjelica, Jelena and Jovanović, Ivan B. and Ilić, Vesna and Valcić, Olivera and Kirovski, Danijela and Gvozdić, Dragan and Milanović, Svetlana",
year = "2017",
abstract = "Both selenium, as an effector and regulator of antioxidative enzymes activity, and thyroid hormones are potent immunomodulators. Besides, selenium incorporated into iodothyronine deiodinases is involved in the thyroid function and thus indirectly regulates the immune response. Studies of the mutual infl uence of selenium and thyroid hormones on the immune response are scarce, hence we analyzed the effects of an iodothyronine deiodinases blocker, propylthiouracil (PTU), and selenium defi ciency on the function of peritoneal macrophages, and titer of naturally occurring anti-sheep red blood cells (SRBC) IgM antibodies in juvenile rats. The experiment was carried out on 64 Wistar male rats allotted to 4 groups: controlselenium adequate PTU-group; selenium adequate, PTU+ group; selenium defi cient, PTU-group; and selenium defi cient, PTU+. The selenium adequate and selenium defi cient groups were fed a diet containing 0.334 and 0.031 mg Se/kg, respectively. PTU+ groups received PTU (150 mg/L) in drinking water. After 3 weeks, thyroxine (T-4), triiodothyronine (T-3), and thyroid stimulating hormone (TSH) were determined. The animals having "intermediate" concentrations of T-3 (1.56-1.69 nmol/L) and T 4 (41-50 nmol/L) were excluded from further analysis. Thus, PTU+ groups included hypothyroid animals (T-3  lt = 1.55 nmol/L; T-4  lt = 40 nmol/L), while PTU-groups included euthyroid rats (T-3  lt = 1.70 nmol/L; T-4  lt = 50 nmol/L). Both groups of selenium defi cient rats had, when compared to the control group, a signifi cantly lower activity of glutathione peroxidase GPx1 and GPx3. Neither selenium defi ciency nor PTU infl uenced the adherence of peritoneal macrophages. Selenium defi ciency signifi cantly decreased the peroxide synthesis in macrophages and signifi cantly increased the titer of anti-SRBC IgM. Hypotyroidism alone or in combination with selenium defi ciency had no infl uence on these parameters.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats",
pages = "355-340",
number = "3",
volume = "67",
doi = "10.1515/acve-2017-0028"
}

Bjelica, J., Jovanović, I. B., Ilić, V., Valcić, O., Kirovski, D., Gvozdić, D.,& Milanović, S.. (2017). Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(3), 340-355.
https://doi.org/10.1515/acve-2017-0028

Bjelica J, Jovanović IB, Ilić V, Valcić O, Kirovski D, Gvozdić D, Milanović S. Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats. in Acta Veterinaria-Beograd. 2017;67(3):340-355.
doi:10.1515/acve-2017-0028 .

Bjelica, Jelena, Jovanović, Ivan B., Ilić, Vesna, Valcić, Olivera, Kirovski, Danijela, Gvozdić, Dragan, Milanović, Svetlana, "Effects of selenium and thyroid hormone deficiency on peritoneal macrophages adhesion and occurrence of natural IGM antibodies in juvenile rats" in Acta Veterinaria-Beograd, 67, no. 3 (2017):340-355,
https://doi.org/10.1515/acve-2017-0028 . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Gvozdić, Dragan
AU  - Vuković, Dejan
AU  - Savić, Olivera
AU  - Kovačić, Marijana
AU  - Ilić, Vesna
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/399
AB  - Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology & Immunopathology
T1  - Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG
EP  - 168
IS  - 3-4
SP  - 161
VL  - 150
DO  - 10.1016/j.vetimm.2012.09.009
ER  - 

@article{
author = "Fratrić, Natalija and Gvozdić, Dragan and Vuković, Dejan and Savić, Olivera and Kovačić, Marijana and Ilić, Vesna",
year = "2012",
abstract = "Immune complexes (IC) could have an important role in the pathogenesis of pre-ruminant calves' bronchopneumonia. IC are potent activators of complement and neutrophils and they might be responsible for immune protection, as well as for pulmonary damage. Immunoglobulin G (IgG), as constituents of IC, initiates the effector phase of immune response through binding of Fc gamma and complement receptors. The oligosaccharide moieties expressed on IgG can modulate their antigen affinity and effector function. Structural characteristics of IgG molecules from IC in the pre-ruminant calves have not been studied in detail. The aim of our study was to determine if the glycosylation profile of IgG from circulating IC (CIC) in calves with bronchopneumonia differed from those of healthy control calves. A total number of 13 Holstein-Friesian calves, at the age of three months were included in the study. All calves were clinically examined by a veterinarian. Calves were classified by signs of respiratory disease in two groups: healthy (n = 6) and diseased (n = 7) calves. The CIC from calves' sera were isolated by the polyethylene glycol precipitation (PEG) method. IgG molecules were isolated from PEG precipitates by Protein G affinity method. The level of expression and localization N-acetylglucosamine, galactose, sialic acid, and fucose within the isolated IgG was determined by lectin blot assay. Calves with bronchopneumonia had a statistically significantly increased level of CIC. IgG molecule:, were isolated from CIC of both healthy and diseased calves. Several other proteins in complex with IgG were detected in both groups of animals. The isolated IgG heavy chains of healthy calves expressed N-acetylglucosamine, galactose, sialic acid, and fucose. The light chains of IgG expressed N-acetylglucosamine, sialic acid, and fucose whereas galactose was not detected in healthy calves. In diseased animals, galactose was detected on light chains, and both heavy and light IgG chains were more sialylated. Proteins in complex with IgG were also lectin reactive, and their glycosylation in diseased animals was different compared to healthy controls. Increased sialylation is a characteristic of anti-inflammatory IgG. The increased sialylation of IgG from CIC in bronchopneumonia might be an attempt of immune system of calves to protect lung tissues against damages provoked by activated cells and secreted pro-inflammatory cytokines. At the same time, increased IgG sialylation could explain the inability of calves' immune system to initiate the process of antigen elimination by activation of Fc gamma receptors.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology & Immunopathology",
title = "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG",
pages = "168-161",
number = "3-4",
volume = "150",
doi = "10.1016/j.vetimm.2012.09.009"
}

Fratrić, N., Gvozdić, D., Vuković, D., Savić, O., Kovačić, M.,& Ilić, V.. (2012). Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology
Elsevier, Amsterdam., 150(3-4), 161-168.
https://doi.org/10.1016/j.vetimm.2012.09.009

Fratrić N, Gvozdić D, Vuković D, Savić O, Kovačić M, Ilić V. Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG. in Veterinary Immunology & Immunopathology. 2012;150(3-4):161-168.
doi:10.1016/j.vetimm.2012.09.009 .

Fratrić, Natalija, Gvozdić, Dragan, Vuković, Dejan, Savić, Olivera, Kovačić, Marijana, Ilić, Vesna, "Evidence that calf bronchopneumonia may be accompanied by increased sialylation of circulating immune complexes' IgG" in Veterinary Immunology & Immunopathology, 150, no. 3-4 (2012):161-168,
https://doi.org/10.1016/j.vetimm.2012.09.009 . .