TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1346
AB  - Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T2  - Biomolecules
T1  - The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis
IS  - 10
SP  - 1437
VL  - 13
DO  - 10.3390/biom13101437
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules, Biomolecules",
title = "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis",
number = "10",
pages = "1437",
volume = "13",
doi = "10.3390/biom13101437"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 13(10), 1437.
https://doi.org/10.3390/biom13101437

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules. 2023;13(10):1437.
doi:10.3390/biom13101437 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis" in Biomolecules, 13, no. 10 (2023):1437,
https://doi.org/10.3390/biom13101437 . .

TY  - CONF
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1406
AB  - Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.
PB  - Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije
C3  - Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
T1  - Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1406
ER  - 

@conference{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.",
publisher = "Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije",
journal = "Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka",
title = "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1406"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije..
https://hdl.handle.net/21.15107/rcub_rimi_1406

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka. 2023;.
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma" in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka (2023),
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Živanović, Milena
AU  - Vujačić, Marko
AU  - Bogosavljević, Nikola
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1409
AB  - Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation
IS  - 3
SP  - P141
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1409
ER  - 

@conference{
author = "Trivanović, Drenka and Okić Đorđević, Ivana and Živanović, Milena and Vujačić, Marko and Bogosavljević, Nikola and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation",
number = "3",
pages = "P141",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1409"
}

Trivanović, D., Okić Đorđević, I., Živanović, M., Vujačić, M., Bogosavljević, N., Bugarski, D.,& Jauković, A.. (2023). Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409

Trivanović D, Okić Đorđević I, Živanović M, Vujačić M, Bogosavljević N, Bugarski D, Jauković A. Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

Trivanović, Drenka, Okić Đorđević, Ivana, Živanović, Milena, Vujačić, Marko, Bogosavljević, Nikola, Bugarski, Diana, Jauković, Aleksandra, "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P141,
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Miletić, Maja
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1184
AB  - Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
PB  - Srpsko biološko društvo
T2  - Archives of Biological Sciences
T1  - Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
EP  - 24
IS  - 1
SP  - 15
VL  - 74
DO  - 10.2298/ABS210929048O
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Trivanović, Drenka and Petrović, Anđelija and Mojsilović, Slavko and Miletić, Maja and Bugarski, Diana and Jauković, Aleksandra",
year = "2022",
abstract = "Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.",
publisher = "Srpsko biološko društvo",
journal = "Archives of Biological Sciences",
title = "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway",
pages = "24-15",
number = "1",
volume = "74",
doi = "10.2298/ABS210929048O"
}

Okić Đorđević, I., Kukolj, T., Obradović, H., Trivanović, D., Petrović, A., Mojsilović, S., Miletić, M., Bugarski, D.,& Jauković, A.. (2022). Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences
Srpsko biološko društvo., 74(1), 15-24.
https://doi.org/10.2298/ABS210929048O

Okić Đorđević I, Kukolj T, Obradović H, Trivanović D, Petrović A, Mojsilović S, Miletić M, Bugarski D, Jauković A. Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences. 2022;74(1):15-24.
doi:10.2298/ABS210929048O .

Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Trivanović, Drenka, Petrović, Anđelija, Mojsilović, Slavko, Miletić, Maja, Bugarski, Diana, Jauković, Aleksandra, "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway" in Archives of Biological Sciences, 74, no. 1 (2022):15-24,
https://doi.org/10.2298/ABS210929048O . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Miletić, Maja
AU  - Petrović, Vanja
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1149
AB  - Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
PB  - Wiley-Blackwell
T2  - Journal of Cellular Physiology
T1  - Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
EP  - 7341
IS  - 11
SP  - 7322
VL  - 236
DO  - 10.1002/jcp.30399
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Obradović, Hristina and Miletić, Maja and Petrović, Vanja and Mojsilović, Slavko and Bugarski, Diana",
year = "2021",
abstract = "Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.",
publisher = "Wiley-Blackwell",
journal = "Journal of Cellular Physiology",
title = "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF",
pages = "7341-7322",
number = "11",
volume = "236",
doi = "10.1002/jcp.30399"
}

Jauković, A., Kukolj, T., Trivanović, D., Okić Đorđević, I., Obradović, H., Miletić, M., Petrović, V., Mojsilović, S.,& Bugarski, D.. (2021). Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology
Wiley-Blackwell., 236(11), 7322-7341.
https://doi.org/10.1002/jcp.30399

Jauković A, Kukolj T, Trivanović D, Okić Đorđević I, Obradović H, Miletić M, Petrović V, Mojsilović S, Bugarski D. Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology. 2021;236(11):7322-7341.
doi:10.1002/jcp.30399 .

Jauković, Aleksandra, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Obradović, Hristina, Miletić, Maja, Petrović, Vanja, Mojsilović, Slavko, Bugarski, Diana, "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF" in Journal of Cellular Physiology, 236, no. 11 (2021):7322-7341,
https://doi.org/10.1002/jcp.30399 . .

TY  - JOUR
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Petrović, Anđelija
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1175
AB  - As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Journal of Personalized Medicine
T1  - Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy
IS  - 11
SP  - 1133
VL  - 11
DO  - 10.3390/jpm11111133
ER  - 

@article{
author = "Mojsilović, Slavko and Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Petrović, Anđelija and Bugarski, Diana",
year = "2021",
abstract = "As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Journal of Personalized Medicine",
title = "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy",
number = "11",
pages = "1133",
volume = "11",
doi = "10.3390/jpm11111133"
}

Mojsilović, S., Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Petrović, A.,& Bugarski, D.. (2021). Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine
Multidisciplinary Digital Publishing Institute (MDPI)., 11(11), 1133.
https://doi.org/10.3390/jpm11111133

Mojsilović S, Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Petrović A, Bugarski D. Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine. 2021;11(11):1133.
doi:10.3390/jpm11111133 .

Mojsilović, Slavko, Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Petrović, Anđelija, Bugarski, Diana, "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy" in Journal of Personalized Medicine, 11, no. 11 (2021):1133,
https://doi.org/10.3390/jpm11111133 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Kukolj, Tamara
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1183
AB  - Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.
PB  - Baishideng Publishing Group Inc
T2  - World Journal of Stem Cells
T1  - Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy
EP  - 1880
IS  - 12
SP  - 1863
VL  - 13
DO  - 10.4252/wjsc.v13.i12.1863
ER  - 

@article{
author = "Okić Đorđević, Ivana and Obradović, Hristina and Kukolj, Tamara and Petrović, Anđelija and Mojsilović, Slavko and Bugarski, Diana and Jauković, Aleksandra",
year = "2021",
abstract = "Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.",
publisher = "Baishideng Publishing Group Inc",
journal = "World Journal of Stem Cells",
title = "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy",
pages = "1880-1863",
number = "12",
volume = "13",
doi = "10.4252/wjsc.v13.i12.1863"
}

Okić Đorđević, I., Obradović, H., Kukolj, T., Petrović, A., Mojsilović, S., Bugarski, D.,& Jauković, A.. (2021). Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells
Baishideng Publishing Group Inc., 13(12), 1863-1880.
https://doi.org/10.4252/wjsc.v13.i12.1863

Okić Đorđević I, Obradović H, Kukolj T, Petrović A, Mojsilović S, Bugarski D, Jauković A. Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells. 2021;13(12):1863-1880.
doi:10.4252/wjsc.v13.i12.1863 .

Okić Đorđević, Ivana, Obradović, Hristina, Kukolj, Tamara, Petrović, Anđelija, Mojsilović, Slavko, Bugarski, Diana, Jauković, Aleksandra, "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy" in World Journal of Stem Cells, 13, no. 12 (2021):1863-1880,
https://doi.org/10.4252/wjsc.v13.i12.1863 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/993
AB  - Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Stem Cells
T1  - Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators
EP  - 937
IS  - 9
SP  - 922
VL  - 12
DO  - 10.4252/wjsc.v12.i9.922
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Bugarski, Diana",
year = "2020",
abstract = "Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Stem Cells",
title = "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators",
pages = "937-922",
number = "9",
volume = "12",
doi = "10.4252/wjsc.v12.i9.922"
}

Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S.,& Bugarski, D.. (2020). Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells
Baishideng Publishing Group Inc, Pleasanton., 12(9), 922-937.
https://doi.org/10.4252/wjsc.v12.i9.922

Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Bugarski D. Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells. 2020;12(9):922-937.
doi:10.4252/wjsc.v12.i9.922 .

Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Bugarski, Diana, "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators" in World Journal of Stem Cells, 12, no. 9 (2020):922-937,
https://doi.org/10.4252/wjsc.v12.i9.922 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Santibanez, Juan F.
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1003
AB  - Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function.
AB  - Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts
T1  - Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta
EP  - 33
IS  - 1
SP  - 18
VL  - 74
DO  - 10.2298/VETGL190414019M
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Stančić, Ana and Santibanez, Juan F. and Trivanović, Drenka and Drvenica, Ivana and Krstić, Jelena and Mojsilović, Slavko and Okić Đorđević, Ivana and Bugarski, Diana",
year = "2020",
abstract = "Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function., Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts, Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta",
pages = "33-18",
number = "1",
volume = "74",
doi = "10.2298/VETGL190414019M"
}

Maslovarić, I., Ilić, V., Stančić, A., Santibanez, J. F., Trivanović, D., Drvenica, I., Krstić, J., Mojsilović, S., Okić Đorđević, I.,& Bugarski, D.. (2020). Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(1), 18-33.
https://doi.org/10.2298/VETGL190414019M

Maslovarić I, Ilić V, Stančić A, Santibanez JF, Trivanović D, Drvenica I, Krstić J, Mojsilović S, Okić Đorđević I, Bugarski D. Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik. 2020;74(1):18-33.
doi:10.2298/VETGL190414019M .

Maslovarić, Irina, Ilić, Vesna, Stančić, Ana, Santibanez, Juan F., Trivanović, Drenka, Drvenica, Ivana, Krstić, Jelena, Mojsilović, Slavko, Okić Đorđević, Ivana, Bugarski, Diana, "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts" in Veterinarski glasnik, 74, no. 1 (2020):18-33,
https://doi.org/10.2298/VETGL190414019M . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Vignjević-Petrinović, Sanja
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/995
AB  - Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cell & Developmental Biology
T1  - Adipogenesis in Different Body Depots and Tumor Development
VL  - 8
DO  - 10.3389/fcell.2020.571648
ER  - 

@article{
author = "Trivanović, Drenka and Vignjević-Petrinović, Sanja and Okić Đorđević, Ivana and Kukolj, Tamara and Bugarski, Diana and Jauković, Aleksandra",
year = "2020",
abstract = "Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cell & Developmental Biology",
title = "Adipogenesis in Different Body Depots and Tumor Development",
volume = "8",
doi = "10.3389/fcell.2020.571648"
}

Trivanović, D., Vignjević-Petrinović, S., Okić Đorđević, I., Kukolj, T., Bugarski, D.,& Jauković, A.. (2020). Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fcell.2020.571648

Trivanović D, Vignjević-Petrinović S, Okić Đorđević I, Kukolj T, Bugarski D, Jauković A. Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology. 2020;8.
doi:10.3389/fcell.2020.571648 .

Trivanović, Drenka, Vignjević-Petrinović, Sanja, Okić Đorđević, Ivana, Kukolj, Tamara, Bugarski, Diana, Jauković, Aleksandra, "Adipogenesis in Different Body Depots and Tumor Development" in Frontiers in Cell & Developmental Biology, 8 (2020),
https://doi.org/10.3389/fcell.2020.571648 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/950
AB  - Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.
PB  - Wiley, Hoboken
T2  - Cell Proliferation
T1  - IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells
IS  - 1
VL  - 52
DO  - 10.1111/cpr.12533
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Mojsilović, Slavko and Okić Đorđević, Ivana and Obradović, Hristina and Krstić, Jelena and Jauković, Aleksandra and Bugarski, Diana",
year = "2019",
abstract = "Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.",
publisher = "Wiley, Hoboken",
journal = "Cell Proliferation",
title = "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells",
number = "1",
volume = "52",
doi = "10.1111/cpr.12533"
}

Kukolj, T., Trivanović, D., Mojsilović, S., Okić Đorđević, I., Obradović, H., Krstić, J., Jauković, A.,& Bugarski, D.. (2019). IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation
Wiley, Hoboken., 52(1).
https://doi.org/10.1111/cpr.12533

Kukolj T, Trivanović D, Mojsilović S, Okić Đorđević I, Obradović H, Krstić J, Jauković A, Bugarski D. IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation. 2019;52(1).
doi:10.1111/cpr.12533 .

Kukolj, Tamara, Trivanović, Drenka, Mojsilović, Slavko, Okić Đorđević, Ivana, Obradović, Hristina, Krstić, Jelena, Jauković, Aleksandra, Bugarski, Diana, "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells" in Cell Proliferation, 52, no. 1 (2019),
https://doi.org/10.1111/cpr.12533 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić, Ivana
AU  - Kukolj, Tamara
AU  - Ilić, Vesna
AU  - Jauković, Aleksandra
AU  - Terzić, Milan
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/969
AB  - Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.
PB  - W B Saunders Co Ltd, London
T2  - Placenta
T1  - Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche
EP  - 34
SP  - 25
VL  - 82
DO  - 10.1016/j.placenta.2019.05.005
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Trivanović, Drenka and Mojsilović, Slavko and Okić, Ivana and Kukolj, Tamara and Ilić, Vesna and Jauković, Aleksandra and Terzić, Milan and Bugarski, Diana",
year = "2019",
abstract = "Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.",
publisher = "W B Saunders Co Ltd, London",
journal = "Placenta",
title = "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche",
pages = "34-25",
volume = "82",
doi = "10.1016/j.placenta.2019.05.005"
}

Obradović, H., Krstić, J., Trivanović, D., Mojsilović, S., Okić, I., Kukolj, T., Ilić, V., Jauković, A., Terzić, M.,& Bugarski, D.. (2019). Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta
W B Saunders Co Ltd, London., 82, 25-34.
https://doi.org/10.1016/j.placenta.2019.05.005

Obradović H, Krstić J, Trivanović D, Mojsilović S, Okić I, Kukolj T, Ilić V, Jauković A, Terzić M, Bugarski D. Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta. 2019;82:25-34.
doi:10.1016/j.placenta.2019.05.005 .

Obradović, Hristina, Krstić, Jelena, Trivanović, Drenka, Mojsilović, Slavko, Okić, Ivana, Kukolj, Tamara, Ilić, Vesna, Jauković, Aleksandra, Terzić, Milan, Bugarski, Diana, "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche" in Placenta, 82 (2019):25-34,
https://doi.org/10.1016/j.placenta.2019.05.005 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/835
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine & Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine & Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine & Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Obradović, Hristina
AU  - Janković, Srdja
AU  - Santibanez, Juan F.
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/873
AB  - Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (Escherichia coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability, and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP, and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPAR mRNA expression. LPS promoted myofibroblast-like phenotype of PDLSCs, since it significantly enhanced PDLSC contractility, as well as protein and/or gene expression of TGF-beta, fibronectin (FN), alpha-SMA, and NG2. LPS also increased protein and gene expression levels of anti-inflammatory COX-2 and pro-inflammatory IL-6 molecules in PDLSCs. Inhibition of peripheral blood mononuclear cells (MNCs) transendothelial migration in presence of LPS-treated PDLSCs was accompanied by the reduction of CD29 expression within MNCs. However, LPS treatment did not change the inhibitory effect of PDLSCs on mitogen-stimulated proliferation of CD4(+) and the ratio of CD4(+)CD25(high)/CD4(+)CD25(low) lymphocytes. LPS-treated PDLSCs did not change the frequency of CD34(+) and CD45(+) cells, but decreased the frequency of CD33(+) and CD14(+) myeloid cells within MNCs. Moreover, LPS treatment attenuated the stimulatory effect of PDLSCs on CFC activity of MNCs, predominantly the CFU-GM number. The results indicated that LPS-activated ERK1,2 was at least partly involved in the observed effects on PDLSC differentiation capacity, acquisition of myofibroblastic attributes, and changes of their immunomodulatory features.
PB  - Wiley, Hoboken
T2  - Journal of Cellular Physiology
T1  - Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling
EP  - 462
IS  - 1
SP  - 447
VL  - 233
DO  - 10.1002/jcp.25904
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Obradović, Hristina and Janković, Srdja and Santibanez, Juan F. and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (Escherichia coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability, and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP, and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPAR mRNA expression. LPS promoted myofibroblast-like phenotype of PDLSCs, since it significantly enhanced PDLSC contractility, as well as protein and/or gene expression of TGF-beta, fibronectin (FN), alpha-SMA, and NG2. LPS also increased protein and gene expression levels of anti-inflammatory COX-2 and pro-inflammatory IL-6 molecules in PDLSCs. Inhibition of peripheral blood mononuclear cells (MNCs) transendothelial migration in presence of LPS-treated PDLSCs was accompanied by the reduction of CD29 expression within MNCs. However, LPS treatment did not change the inhibitory effect of PDLSCs on mitogen-stimulated proliferation of CD4(+) and the ratio of CD4(+)CD25(high)/CD4(+)CD25(low) lymphocytes. LPS-treated PDLSCs did not change the frequency of CD34(+) and CD45(+) cells, but decreased the frequency of CD33(+) and CD14(+) myeloid cells within MNCs. Moreover, LPS treatment attenuated the stimulatory effect of PDLSCs on CFC activity of MNCs, predominantly the CFU-GM number. The results indicated that LPS-activated ERK1,2 was at least partly involved in the observed effects on PDLSC differentiation capacity, acquisition of myofibroblastic attributes, and changes of their immunomodulatory features.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling",
pages = "462-447",
number = "1",
volume = "233",
doi = "10.1002/jcp.25904"
}

Kukolj, T., Trivanović, D., Okić Đorđević, I., Mojsilović, S., Krstić, J., Obradović, H., Janković, S., Santibanez, J. F., Jauković, A.,& Bugarski, D.. (2018). Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. in Journal of Cellular Physiology
Wiley, Hoboken., 233(1), 447-462.
https://doi.org/10.1002/jcp.25904

Kukolj T, Trivanović D, Okić Đorđević I, Mojsilović S, Krstić J, Obradović H, Janković S, Santibanez JF, Jauković A, Bugarski D. Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. in Journal of Cellular Physiology. 2018;233(1):447-462.
doi:10.1002/jcp.25904 .

Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Obradović, Hristina, Janković, Srdja, Santibanez, Juan F., Jauković, Aleksandra, Bugarski, Diana, "Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling" in Journal of Cellular Physiology, 233, no. 1 (2018):447-462,
https://doi.org/10.1002/jcp.25904 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Santibanez, Juan F.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/710
AB  - Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 inmyoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulateMMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.
PB  - Hindawi Ltd, London
T2  - Mediators of Inflammation
T1  - Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation
VL  - 2016
DO  - 10.1155/2016/2939658
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Mojsilović, Slavko and Jauković, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Santibanez, Juan F.",
year = "2016",
abstract = "Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 inmyoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulateMMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.",
publisher = "Hindawi Ltd, London",
journal = "Mediators of Inflammation",
title = "Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation",
volume = "2016",
doi = "10.1155/2016/2939658"
}

Obradović, H., Krstić, J., Kukolj, T., Trivanović, D., Okić Đorđević, I., Mojsilović, S., Jauković, A., Jovčić, G., Bugarski, D.,& Santibanez, J. F.. (2016). Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation. in Mediators of Inflammation
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/2939658

Obradović H, Krstić J, Kukolj T, Trivanović D, Okić Đorđević I, Mojsilović S, Jauković A, Jovčić G, Bugarski D, Santibanez JF. Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation. in Mediators of Inflammation. 2016;2016.
doi:10.1155/2016/2939658 .

Obradović, Hristina, Krstić, Jelena, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Mojsilović, Slavko, Jauković, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Santibanez, Juan F., "Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation" in Mediators of Inflammation, 2016 (2016),
https://doi.org/10.1155/2016/2939658 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Krstić, Jelena
AU  - Nikolić, Srdjan
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Santibanez, Juan F.
AU  - Bugarski, Diana
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/726
AB  - Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-gamma (IFN-gamma) and/or tumor necrosis factor-alpha (TNF-alpha) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro. IFN-gamma and/or TNF-alpha-primed hASCs produced conditioned media (CM) which induced epithelial to mesenchymal transition (EMT) of MCF-7 cells by reducing E-Cadherin and increasing Vimentin expression. Induced EMT was accompanied by increased invasion, migration, and urokinase type-plasminogen activator (uPA) expression in MCF-7 cells. These effects were mediated by increased expression of transforming growth factor-beta 1(TGF-beta 1) in cytokines-primed hASCs, since inhibition of type I TGF-beta 1 receptor on MCF-7 cells and neutralization of TGF-beta 1 disabled the CM from primed hASCs to increase EMT, cell migration, and uPA expression in MCF-7 cells. Obtained data suggested that IFN-gamma and/or TNF-alpha primed hASCs might enhance the malignancy of MCF-7 cell line by inducing EMT, cell motility and uPA expression in these cells via TGF-beta 1-Smad3 signalization, with potentially important implications in breast cancer progression.
PB  - Wiley, Hoboken
T2  - Iubmb Life
T1  - Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1
EP  - 200
IS  - 3
SP  - 190
VL  - 68
DO  - 10.1002/iub.1473
ER  - 

@article{
author = "Trivanović, Drenka and Jauković, Aleksandra and Krstić, Jelena and Nikolić, Srdjan and Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Mojsilović, Slavko and Ilić, Vesna and Santibanez, Juan F. and Bugarski, Diana",
year = "2016",
abstract = "Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-gamma (IFN-gamma) and/or tumor necrosis factor-alpha (TNF-alpha) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro. IFN-gamma and/or TNF-alpha-primed hASCs produced conditioned media (CM) which induced epithelial to mesenchymal transition (EMT) of MCF-7 cells by reducing E-Cadherin and increasing Vimentin expression. Induced EMT was accompanied by increased invasion, migration, and urokinase type-plasminogen activator (uPA) expression in MCF-7 cells. These effects were mediated by increased expression of transforming growth factor-beta 1(TGF-beta 1) in cytokines-primed hASCs, since inhibition of type I TGF-beta 1 receptor on MCF-7 cells and neutralization of TGF-beta 1 disabled the CM from primed hASCs to increase EMT, cell migration, and uPA expression in MCF-7 cells. Obtained data suggested that IFN-gamma and/or TNF-alpha primed hASCs might enhance the malignancy of MCF-7 cell line by inducing EMT, cell motility and uPA expression in these cells via TGF-beta 1-Smad3 signalization, with potentially important implications in breast cancer progression.",
publisher = "Wiley, Hoboken",
journal = "Iubmb Life",
title = "Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1",
pages = "200-190",
number = "3",
volume = "68",
doi = "10.1002/iub.1473"
}

Trivanović, D., Jauković, A., Krstić, J., Nikolić, S., Okić Đorđević, I., Kukolj, T., Obradović, H., Mojsilović, S., Ilić, V., Santibanez, J. F.,& Bugarski, D.. (2016). Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1. in Iubmb Life
Wiley, Hoboken., 68(3), 190-200.
https://doi.org/10.1002/iub.1473

Trivanović D, Jauković A, Krstić J, Nikolić S, Okić Đorđević I, Kukolj T, Obradović H, Mojsilović S, Ilić V, Santibanez JF, Bugarski D. Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1. in Iubmb Life. 2016;68(3):190-200.
doi:10.1002/iub.1473 .

Trivanović, Drenka, Jauković, Aleksandra, Krstić, Jelena, Nikolić, Srdjan, Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Mojsilović, Slavko, Ilić, Vesna, Santibanez, Juan F., Bugarski, Diana, "Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1" in Iubmb Life, 68, no. 3 (2016):190-200,
https://doi.org/10.1002/iub.1473 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Obradović, Hristina
AU  - Santibanez, Juan F.
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/731
AB  - Periodontal disease (PD), a degenerative bacterially induced disease of periodontium, can lead to bone resorption and teeth loss. Development of PD includes a strong inflammatory reaction, which involves multiple immune cells and their secreting factors including interleukin-17 (IL-17), which is not only an important modulator of immune and hematopoietic responses but also affects bone metabolism. In the present study we aimed to determine whether IL-17 affects the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) by investigating its ability to modulate osteogenic differentiation of these cells in vitro along with associated signaling pathways. Our results revealed that IL-17 inhibited both the proliferation and migration of PDLSCs and decreased their osteogenic differentiation by activating ERK1,2 and JNK mitogen-activated protein kinases. Obtained data suggested that IL-17 might contribute to alveolar bone loss in PD.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - International Journal of Biochemistry & Cell Biology
T1  - The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs
EP  - 101
SP  - 92
VL  - 71
DO  - 10.1016/j.biocel.2015.12.007
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Krstić, Jelena and Trivanović, Drenka and Obradović, Hristina and Santibanez, Juan F. and Mojsilović, Slavko and Ilić, Vesna and Bugarski, Diana and Jauković, Aleksandra",
year = "2016",
abstract = "Periodontal disease (PD), a degenerative bacterially induced disease of periodontium, can lead to bone resorption and teeth loss. Development of PD includes a strong inflammatory reaction, which involves multiple immune cells and their secreting factors including interleukin-17 (IL-17), which is not only an important modulator of immune and hematopoietic responses but also affects bone metabolism. In the present study we aimed to determine whether IL-17 affects the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) by investigating its ability to modulate osteogenic differentiation of these cells in vitro along with associated signaling pathways. Our results revealed that IL-17 inhibited both the proliferation and migration of PDLSCs and decreased their osteogenic differentiation by activating ERK1,2 and JNK mitogen-activated protein kinases. Obtained data suggested that IL-17 might contribute to alveolar bone loss in PD.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "International Journal of Biochemistry & Cell Biology",
title = "The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs",
pages = "101-92",
volume = "71",
doi = "10.1016/j.biocel.2015.12.007"
}

Okić Đorđević, I., Kukolj, T., Krstić, J., Trivanović, D., Obradović, H., Santibanez, J. F., Mojsilović, S., Ilić, V., Bugarski, D.,& Jauković, A.. (2016). The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs. in International Journal of Biochemistry & Cell Biology
Pergamon-Elsevier Science Ltd, Oxford., 71, 92-101.
https://doi.org/10.1016/j.biocel.2015.12.007

Okić Đorđević I, Kukolj T, Krstić J, Trivanović D, Obradović H, Santibanez JF, Mojsilović S, Ilić V, Bugarski D, Jauković A. The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs. in International Journal of Biochemistry & Cell Biology. 2016;71:92-101.
doi:10.1016/j.biocel.2015.12.007 .

Okić Đorđević, Ivana, Kukolj, Tamara, Krstić, Jelena, Trivanović, Drenka, Obradović, Hristina, Santibanez, Juan F., Mojsilović, Slavko, Ilić, Vesna, Bugarski, Diana, Jauković, Aleksandra, "The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs" in International Journal of Biochemistry & Cell Biology, 71 (2016):92-101,
https://doi.org/10.1016/j.biocel.2015.12.007 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Krstić, Jelena
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Santibanez, Juan F.
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/744
AB  - State of tumor microenvironment (TME) is closely linked to regulation of tumor growth and progression affecting the final outcome, refractoriness, and relapse of disease. Interactions of tumor, immune, and mesenchymal stromal/stem cells (MSCs) have been recognized as crucial for understanding tumorigenesis. Due to their outstanding features, stem cell-like properties, capacity to regulate immune response, and dynamic functional phenotype dependent on microenvironmental stimuli, MSCs have been perceived as important players in TME. Signals provided by tumor-associated chronic inflammation educate MSCs to alter their phenotype and immunomodulatory potential in favor of tumor-biased state of MSCs. Adjustment of phenotype to TME and acquisition of tumor-promoting ability byMSCs help tumor cells inmaintenance of permissive TME and suppression of antitumor immune response. Potential utilization of MSCs in treatment of tumor is based on their inherent ability to home tumor tissue that makes them suitable delivery vehicles for immune-stimulating factors and vectors for targeted antitumor therapy. Here, we review data regarding intrusive effects of inflammatory TME on MSCs capacity to affect tumor development through modification of their phenotype and interactions with immune system.
PB  - Hindawi Ltd, London
T2  - Mediators of Inflammation
T1  - The Roles of Mesenchymal Stromal/Stem Cells in Tumor Microenvironment Associated with Inflammation
VL  - 2016
DO  - 10.1155/2016/7314016
ER  - 

@article{
author = "Trivanović, Drenka and Krstić, Jelena and Okić Đorđević, Ivana and Mojsilović, Slavko and Santibanez, Juan F. and Bugarski, Diana and Jauković, Aleksandra",
year = "2016",
abstract = "State of tumor microenvironment (TME) is closely linked to regulation of tumor growth and progression affecting the final outcome, refractoriness, and relapse of disease. Interactions of tumor, immune, and mesenchymal stromal/stem cells (MSCs) have been recognized as crucial for understanding tumorigenesis. Due to their outstanding features, stem cell-like properties, capacity to regulate immune response, and dynamic functional phenotype dependent on microenvironmental stimuli, MSCs have been perceived as important players in TME. Signals provided by tumor-associated chronic inflammation educate MSCs to alter their phenotype and immunomodulatory potential in favor of tumor-biased state of MSCs. Adjustment of phenotype to TME and acquisition of tumor-promoting ability byMSCs help tumor cells inmaintenance of permissive TME and suppression of antitumor immune response. Potential utilization of MSCs in treatment of tumor is based on their inherent ability to home tumor tissue that makes them suitable delivery vehicles for immune-stimulating factors and vectors for targeted antitumor therapy. Here, we review data regarding intrusive effects of inflammatory TME on MSCs capacity to affect tumor development through modification of their phenotype and interactions with immune system.",
publisher = "Hindawi Ltd, London",
journal = "Mediators of Inflammation",
title = "The Roles of Mesenchymal Stromal/Stem Cells in Tumor Microenvironment Associated with Inflammation",
volume = "2016",
doi = "10.1155/2016/7314016"
}

Trivanović, D., Krstić, J., Okić Đorđević, I., Mojsilović, S., Santibanez, J. F., Bugarski, D.,& Jauković, A.. (2016). The Roles of Mesenchymal Stromal/Stem Cells in Tumor Microenvironment Associated with Inflammation. in Mediators of Inflammation
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/7314016

Trivanović D, Krstić J, Okić Đorđević I, Mojsilović S, Santibanez JF, Bugarski D, Jauković A. The Roles of Mesenchymal Stromal/Stem Cells in Tumor Microenvironment Associated with Inflammation. in Mediators of Inflammation. 2016;2016.
doi:10.1155/2016/7314016 .

Trivanović, Drenka, Krstić, Jelena, Okić Đorđević, Ivana, Mojsilović, Slavko, Santibanez, Juan F., Bugarski, Diana, Jauković, Aleksandra, "The Roles of Mesenchymal Stromal/Stem Cells in Tumor Microenvironment Associated with Inflammation" in Mediators of Inflammation, 2016 (2016),
https://doi.org/10.1155/2016/7314016 . .

TY  - THES
AU  - Okić Đorđević, Ivana
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4424
UR  - http://nardus.mpn.gov.rs/handle/123456789/7485
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:14411/bdef:Content/download
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:14567/bdef:Izvestaj/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025143986
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1109
AB  - In the last few decades regenerative and modulatory properties of mesenchymal stem cells (MSCs) have gained the attention of scientists since their characteristics indicated the possibility of their use in regenerative medicine and cell therapy. Although MSCs isolated from various adult and neonatal tissues exhibit the potential of self-renewal and multipotent differentiation (towards cells of mesodermal, ectodermal and endodermal origin), many aspects concerning their functional characteristics are yet to be explored. One of the recently discovered sources of MSCs is easily accessible tissue of periodontal ligament, obtained after tooth extraction for orthodontic reasons. Periodontal ligament tissue strengthens the root of the tooth in the alveolar bone. The regenerative potential of periodontal ligament MSCs (PDLSCs) is reflected in self-renewal and multipotent differentiation ability, as well as potency for the formation of tissues which support the teeth, including the periodontal ligament and cement. These features make the PDLSCs good candidates for use in the regeneration/reconstruction of supporting tissue of teeth in periodontal disease, a chronic inflammatory disease caused by bacterial plaque. The development of chronic periodontal disease includes the process of bone tissue remodeling governed by a number of factors, including osteoblasts and osteoclasts, which reciprocally interact with cellular and humoral compartments of the immune system, thus regulating inflammation, degradation and renewal of bone tissue. The role of the local microenvironment in the periodontal disease is one of the key factors in the context of periodontal ligament tissue response to persistent infection. The presence of T helper cells17 (Th17) cells, important regulators of tissue destruction during inflammatory processes has been confirmed in periodontal lesions. Th17 cells produce proinflammatory cytokine interleukin (IL)-17. In the context of periodontal disease, as well as in other inflammatory diseases, IL-17 stimulates the activation and migration of neutrophils to the site of inflammation. Additionally, IL-17 directly stimulates differentiation of osteoclasts and contributes to the alveolar bone resorption...
AB  - Istraživanja regenerativnih i modulatornih uloga mezenhimskih matičnih ćelija (MMĆ) u poslednjih nekoliko decenija su zaokupirala pažnju naučnika s obzirom da ove ćelije ispoljavaju karakteristike koje ukazuju na mogućnosti njihove primene u regenerativnoj medicini, kao i na polju ćelijske terapije. Iako MMĆ izolovane iz različitih adultnih i neonatalnih izvora ispoljavaju potencijal samoobnove i multipotentne diferencijacije (u ćelije mezodermalnog, ali i ektodermalnog i endodermalnog porekla), mnoga istraživanja koja se tiču funkcionalnih karakteristika ovih ćelija tek predstoje kako bi njihova primena bila efikasna i bezbedna. Jedan od novije otkrivenih izvora MMĆ je i tkivo periodoncijuma koje predstavlja bogat i lako dostupan izvor MMĆ, s obzirom da se ovo tkivo dobija nakon ekstrakcije zuba iz ortodontskih razloga. Periodoncijum je vezivno tkivo čija je glavna uloga uspostavljanje čvrste veze između korena zuba i alveolarne kosti. Regenerativni potencijal MMĆ periodoncijuma (PD-MMĆ) ogleda se u njihovom potencijalu samoobnove i multipotentne diferencijacije, kao i sposobnosti formiranja tkiva sličnih potpornom tkivu zuba, uključujući periodoncijum i cement. Ove osobine čine PD-MMĆ dobrim kandidatima za primenu u regeneraciji potpornog tkiva zuba u parodontopatiji, hroničnom inflamatornom oboljenju uzrokovanom bakterijama zubnog plaka. Razvoj hronične parodontopatije podrazumeva procese remodelovanja koštanog tkiva pod uticajem brojnih faktora u koje su uključeni osteoblasti i osteoklasti koji ostvaruju recipročne interakcije sa ćelijama imunskog odgovora regulišući inflamaciju i degradaciju koštanog tkiva. Uloga lokalne mikrosredine u parodontopatiji predstavlja jedan od ključnih faktora u kontekstu ćelijskog odgovora tkiva periodoncijuma na perzistentnu infekciju. U okviru parodontalnih lezija potvrđeno je prisustvo Th17 (od engl. T helper cells17) ćelija, značajnih regulatora tkivnog razaranja u toku zapaljenskih procesa, koje produkuju proinflamatorni citokin interleukin (IL)-17. U kontekstu parodontopatije, do sada je pokazano da IL-17 ostvaruje svoju ulogu stimulišući privlačenje neutrofila na mesto inflamacije kao i direktnim stimulatornim delovanjem Th17 ćelija na diferencijaciju osteoklasta...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Effects of interleukin-17 on functional properties of human periodontal ligament mesenchymal stem cells
T1  - Efekti interleukina-17 na funkcionalna svojstva humanih mezenhimskih matičnih ćelija periodoncijuma
UR  - https://hdl.handle.net/21.15107/rcub_nardus_7485
ER  - 

@phdthesis{
author = "Okić Đorđević, Ivana",
year = "2016",
abstract = "In the last few decades regenerative and modulatory properties of mesenchymal stem cells (MSCs) have gained the attention of scientists since their characteristics indicated the possibility of their use in regenerative medicine and cell therapy. Although MSCs isolated from various adult and neonatal tissues exhibit the potential of self-renewal and multipotent differentiation (towards cells of mesodermal, ectodermal and endodermal origin), many aspects concerning their functional characteristics are yet to be explored. One of the recently discovered sources of MSCs is easily accessible tissue of periodontal ligament, obtained after tooth extraction for orthodontic reasons. Periodontal ligament tissue strengthens the root of the tooth in the alveolar bone. The regenerative potential of periodontal ligament MSCs (PDLSCs) is reflected in self-renewal and multipotent differentiation ability, as well as potency for the formation of tissues which support the teeth, including the periodontal ligament and cement. These features make the PDLSCs good candidates for use in the regeneration/reconstruction of supporting tissue of teeth in periodontal disease, a chronic inflammatory disease caused by bacterial plaque. The development of chronic periodontal disease includes the process of bone tissue remodeling governed by a number of factors, including osteoblasts and osteoclasts, which reciprocally interact with cellular and humoral compartments of the immune system, thus regulating inflammation, degradation and renewal of bone tissue. The role of the local microenvironment in the periodontal disease is one of the key factors in the context of periodontal ligament tissue response to persistent infection. The presence of T helper cells17 (Th17) cells, important regulators of tissue destruction during inflammatory processes has been confirmed in periodontal lesions. Th17 cells produce proinflammatory cytokine interleukin (IL)-17. In the context of periodontal disease, as well as in other inflammatory diseases, IL-17 stimulates the activation and migration of neutrophils to the site of inflammation. Additionally, IL-17 directly stimulates differentiation of osteoclasts and contributes to the alveolar bone resorption..., Istraživanja regenerativnih i modulatornih uloga mezenhimskih matičnih ćelija (MMĆ) u poslednjih nekoliko decenija su zaokupirala pažnju naučnika s obzirom da ove ćelije ispoljavaju karakteristike koje ukazuju na mogućnosti njihove primene u regenerativnoj medicini, kao i na polju ćelijske terapije. Iako MMĆ izolovane iz različitih adultnih i neonatalnih izvora ispoljavaju potencijal samoobnove i multipotentne diferencijacije (u ćelije mezodermalnog, ali i ektodermalnog i endodermalnog porekla), mnoga istraživanja koja se tiču funkcionalnih karakteristika ovih ćelija tek predstoje kako bi njihova primena bila efikasna i bezbedna. Jedan od novije otkrivenih izvora MMĆ je i tkivo periodoncijuma koje predstavlja bogat i lako dostupan izvor MMĆ, s obzirom da se ovo tkivo dobija nakon ekstrakcije zuba iz ortodontskih razloga. Periodoncijum je vezivno tkivo čija je glavna uloga uspostavljanje čvrste veze između korena zuba i alveolarne kosti. Regenerativni potencijal MMĆ periodoncijuma (PD-MMĆ) ogleda se u njihovom potencijalu samoobnove i multipotentne diferencijacije, kao i sposobnosti formiranja tkiva sličnih potpornom tkivu zuba, uključujući periodoncijum i cement. Ove osobine čine PD-MMĆ dobrim kandidatima za primenu u regeneraciji potpornog tkiva zuba u parodontopatiji, hroničnom inflamatornom oboljenju uzrokovanom bakterijama zubnog plaka. Razvoj hronične parodontopatije podrazumeva procese remodelovanja koštanog tkiva pod uticajem brojnih faktora u koje su uključeni osteoblasti i osteoklasti koji ostvaruju recipročne interakcije sa ćelijama imunskog odgovora regulišući inflamaciju i degradaciju koštanog tkiva. Uloga lokalne mikrosredine u parodontopatiji predstavlja jedan od ključnih faktora u kontekstu ćelijskog odgovora tkiva periodoncijuma na perzistentnu infekciju. U okviru parodontalnih lezija potvrđeno je prisustvo Th17 (od engl. T helper cells17) ćelija, značajnih regulatora tkivnog razaranja u toku zapaljenskih procesa, koje produkuju proinflamatorni citokin interleukin (IL)-17. U kontekstu parodontopatije, do sada je pokazano da IL-17 ostvaruje svoju ulogu stimulišući privlačenje neutrofila na mesto inflamacije kao i direktnim stimulatornim delovanjem Th17 ćelija na diferencijaciju osteoklasta...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Effects of interleukin-17 on functional properties of human periodontal ligament mesenchymal stem cells, Efekti interleukina-17 na funkcionalna svojstva humanih mezenhimskih matičnih ćelija periodoncijuma",
url = "https://hdl.handle.net/21.15107/rcub_nardus_7485"
}

Okić Đorđević, I.. (2016). Effects of interleukin-17 on functional properties of human periodontal ligament mesenchymal stem cells. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_7485

Okić Đorđević I. Effects of interleukin-17 on functional properties of human periodontal ligament mesenchymal stem cells. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_7485 .

Okić Đorđević, Ivana, "Effects of interleukin-17 on functional properties of human periodontal ligament mesenchymal stem cells" (2016),
https://hdl.handle.net/21.15107/rcub_nardus_7485 .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Popović, Branka
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Santibanez, Juan F.
AU  - Bugarski, Diana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/617
AB  - Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression
EP  - 73
SP  - 61
VL  - 141
DO  - 10.1016/j.lfs.2015.09.019
ER  - 

@article{
author = "Trivanović, Drenka and Jauković, Aleksandra and Popović, Branka and Krstić, Jelena and Mojsilović, Slavko and Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Santibanez, Juan F. and Bugarski, Diana",
year = "2015",
abstract = "Aims: In vitro expansion changes replication and differentiation capacity of mesenchymal stem cells (MSCs), increasing challenges and risks, while limiting the sufficient number of MSCs required for cytotherapy. Here, we characterized and compared proliferation, differentiation, telomere length and pluripotency marker expression in MSCs of various origins. Main methods: Immunophenotyping, proliferation and differentiation assays were performed. Pluripotency marker (Nanog, Oct-4, SOX-2, SSEA-4) expression was determined by immunofluorescence. Quantitative PCR was performed for relative telomere length (RTL) analyses, while expression of relevant genes for pluripotency markers, differentiation state (Cbfa1, human placental alkaline phosphatase, peroxisome proliferator activated receptor, Sox9 and Collagen II a1), and telomerase reverse transcriptase (hTERT) was determined by semiquantitative RT-PCR. Key findings: Peripheral blood MSCs (PB-MSCs) and umbilical cord MSCs (UC-MSCs) showed the highest, while periodontal ligament MSCs (PDL-MSCs) and adipose tissue MSCs (AT-MSCs) the lowest values of both the replication potential and RTL. Although MSCs from exfoliated deciduous teeth (SHEDs), PDL-MSCs and AT-MSCs showed higher mRNA expression of pluripotency markers, all MSCs expressed pluripotency marker proteins. SHEDs and PDL-MSCs showed prominent capacity for osteogenesis, PB-MSCs and UC-MSCs showed strengthened adipogenic differentiation potential, while AT-MSCs displayed similar differentiation into both lines. Significance: The MSCs populations derived from different sources, although displaying similar phenotype, exhibited high degree of variability regarding biological properties related to their self-renewal and differentiation capacity. These data indicate that for more accurate use in cell therapy, individualities of MSCs isolated from different tissues should be identified and taken into consideration when planning their use in clinical protocols.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression",
pages = "73-61",
volume = "141",
doi = "10.1016/j.lfs.2015.09.019"
}

Trivanović, D., Jauković, A., Popović, B., Krstić, J., Mojsilović, S., Okić Đorđević, I., Kukolj, T., Obradović, H., Santibanez, J. F.,& Bugarski, D.. (2015). Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 141, 61-73.
https://doi.org/10.1016/j.lfs.2015.09.019

Trivanović D, Jauković A, Popović B, Krstić J, Mojsilović S, Okić Đorđević I, Kukolj T, Obradović H, Santibanez JF, Bugarski D. Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression. in Life Sciences. 2015;141:61-73.
doi:10.1016/j.lfs.2015.09.019 .

Trivanović, Drenka, Jauković, Aleksandra, Popović, Branka, Krstić, Jelena, Mojsilović, Slavko, Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Santibanez, Juan F., Bugarski, Diana, "Mesenchymal stem cells of different origin: Comparative evaluation of proliferative capacity, telomere length and pluripotency marker expression" in Life Sciences, 141 (2015):61-73,
https://doi.org/10.1016/j.lfs.2015.09.019 . .

TY  - JOUR
AU  - Krstić, Jelena
AU  - Obradović, Hristina
AU  - Jauković, Aleksandra
AU  - Okić Đorđević, Ivana
AU  - Trivanović, Drenka
AU  - Kukolj, Tamara
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Santibanez, Juan F.
AU  - Bugarski, Diana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/666
AB  - Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica et Biophysica Acta-Molecular Cell Research
T1  - Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration
EP  - 444
IS  - 2
SP  - 431
VL  - 1853
DO  - 10.1016/j.bbamcr.2014.11.025
ER  - 

@article{
author = "Krstić, Jelena and Obradović, Hristina and Jauković, Aleksandra and Okić Đorđević, Ivana and Trivanović, Drenka and Kukolj, Tamara and Mojsilović, Slavko and Ilić, Vesna and Santibanez, Juan F. and Bugarski, Diana",
year = "2015",
abstract = "Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica et Biophysica Acta-Molecular Cell Research",
title = "Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration",
pages = "444-431",
number = "2",
volume = "1853",
doi = "10.1016/j.bbamcr.2014.11.025"
}

Krstić, J., Obradović, H., Jauković, A., Okić Đorđević, I., Trivanović, D., Kukolj, T., Mojsilović, S., Ilić, V., Santibanez, J. F.,& Bugarski, D.. (2015). Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration. in Biochimica et Biophysica Acta-Molecular Cell Research
Elsevier Science Bv, Amsterdam., 1853(2), 431-444.
https://doi.org/10.1016/j.bbamcr.2014.11.025

Krstić J, Obradović H, Jauković A, Okić Đorđević I, Trivanović D, Kukolj T, Mojsilović S, Ilić V, Santibanez JF, Bugarski D. Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration. in Biochimica et Biophysica Acta-Molecular Cell Research. 2015;1853(2):431-444.
doi:10.1016/j.bbamcr.2014.11.025 .

Krstić, Jelena, Obradović, Hristina, Jauković, Aleksandra, Okić Đorđević, Ivana, Trivanović, Drenka, Kukolj, Tamara, Mojsilović, Slavko, Ilić, Vesna, Santibanez, Juan F., Bugarski, Diana, "Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration" in Biochimica et Biophysica Acta-Molecular Cell Research, 1853, no. 2 (2015):431-444,
https://doi.org/10.1016/j.bbamcr.2014.11.025 . .

TY  - JOUR
AU  - Krstić, Jelena
AU  - Obradović, Hristina
AU  - Kukolj, Tamara
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Bugarski, Diana
AU  - Santibanez, Juan F.
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/679
AB  - Interleukin-17A (IL-17A) and its receptor (IL-17RA) are prototype members of IL-17 ligand/receptor family firstly identified in CD4(+) T cells, which comprises six ligands (IL-17A to IL-17F) and five receptors (IL-17RA to IL-17RE). IL-17A is predominantly secreted by T helper 17 (Th17) cells, and plays important roles in the development of autoimmune and inflammatory diseases. IL-17RA is widely expressed, and forms a complex with IL-17RC. Binding of IL-17A to this receptor complex triggers the activation of several intracellular signaling pathways. In this review, we aimed to summarize literature data about molecular features of IL-17A and IL-17RA from gene to mature protein. We are also providing insight into regulatory mechanisms, protein structural conformation, including ligand-receptor interaction, and an overview of signaling pathways. Our aim was to compile the data on molecular characteristics of IL-17A and IL-17RA which may help in the understanding of their functions in health and disease.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Protein & Peptide Letters
T1  - An Overview of Interleukin-17A and Interleukin-17 Receptor A Structure, Interaction and Signaling
EP  - 578
IS  - 7
SP  - 570
VL  - 22
DO  - 10.2174/0929866522666150520145554
ER  - 

@article{
author = "Krstić, Jelena and Obradović, Hristina and Kukolj, Tamara and Mojsilović, Slavko and Okić Đorđević, Ivana and Bugarski, Diana and Santibanez, Juan F.",
year = "2015",
abstract = "Interleukin-17A (IL-17A) and its receptor (IL-17RA) are prototype members of IL-17 ligand/receptor family firstly identified in CD4(+) T cells, which comprises six ligands (IL-17A to IL-17F) and five receptors (IL-17RA to IL-17RE). IL-17A is predominantly secreted by T helper 17 (Th17) cells, and plays important roles in the development of autoimmune and inflammatory diseases. IL-17RA is widely expressed, and forms a complex with IL-17RC. Binding of IL-17A to this receptor complex triggers the activation of several intracellular signaling pathways. In this review, we aimed to summarize literature data about molecular features of IL-17A and IL-17RA from gene to mature protein. We are also providing insight into regulatory mechanisms, protein structural conformation, including ligand-receptor interaction, and an overview of signaling pathways. Our aim was to compile the data on molecular characteristics of IL-17A and IL-17RA which may help in the understanding of their functions in health and disease.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Protein & Peptide Letters",
title = "An Overview of Interleukin-17A and Interleukin-17 Receptor A Structure, Interaction and Signaling",
pages = "578-570",
number = "7",
volume = "22",
doi = "10.2174/0929866522666150520145554"
}

Krstić, J., Obradović, H., Kukolj, T., Mojsilović, S., Okić Đorđević, I., Bugarski, D.,& Santibanez, J. F.. (2015). An Overview of Interleukin-17A and Interleukin-17 Receptor A Structure, Interaction and Signaling. in Protein & Peptide Letters
Bentham Science Publ Ltd, Sharjah., 22(7), 570-578.
https://doi.org/10.2174/0929866522666150520145554

Krstić J, Obradović H, Kukolj T, Mojsilović S, Okić Đorđević I, Bugarski D, Santibanez JF. An Overview of Interleukin-17A and Interleukin-17 Receptor A Structure, Interaction and Signaling. in Protein & Peptide Letters. 2015;22(7):570-578.
doi:10.2174/0929866522666150520145554 .

Krstić, Jelena, Obradović, Hristina, Kukolj, Tamara, Mojsilović, Slavko, Okić Đorđević, Ivana, Bugarski, Diana, Santibanez, Juan F., "An Overview of Interleukin-17A and Interleukin-17 Receptor A Structure, Interaction and Signaling" in Protein & Peptide Letters, 22, no. 7 (2015):570-578,
https://doi.org/10.2174/0929866522666150520145554 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Nikolić, Srdjan
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Okić Đorđević, Ivana
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/602
AB  - Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.
PB  - Wiley, Hoboken
T2  - Cell Biology International
T1  - Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro
EP  - 265
IS  - 2
SP  - 254
VL  - 38
DO  - 10.1002/cbin.10198
ER  - 

@article{
author = "Trivanović, Drenka and Nikolić, Srdjan and Krstić, Jelena and Jauković, Aleksandra and Mojsilović, Slavko and Ilić, Vesna and Okić Đorđević, Ivana and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.",
publisher = "Wiley, Hoboken",
journal = "Cell Biology International",
title = "Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro",
pages = "265-254",
number = "2",
volume = "38",
doi = "10.1002/cbin.10198"
}

Trivanović, D., Nikolić, S., Krstić, J., Jauković, A., Mojsilović, S., Ilić, V., Okić Đorđević, I., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. in Cell Biology International
Wiley, Hoboken., 38(2), 254-265.
https://doi.org/10.1002/cbin.10198

Trivanović D, Nikolić S, Krstić J, Jauković A, Mojsilović S, Ilić V, Okić Đorđević I, Santibanez JF, Jovčić G, Bugarski D. Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. in Cell Biology International. 2014;38(2):254-265.
doi:10.1002/cbin.10198 .

Trivanović, Drenka, Nikolić, Srdjan, Krstić, Jelena, Jauković, Aleksandra, Mojsilović, Slavko, Ilić, Vesna, Okić Đorđević, Ivana, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro" in Cell Biology International, 38, no. 2 (2014):254-265,
https://doi.org/10.1002/cbin.10198 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Trivanović, Drenka
AU  - Jovanović, Miloš
AU  - Ignjatović, Marija
AU  - Secerov, Bojana
AU  - Mojović, Miloš
AU  - Bugarski, Diana
AU  - Bacić, Goran
AU  - Anđus, Pavle R.
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/592
AB  - Aim To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011) Methods Animals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs. Results In the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by similar to 60% - from 63 to 28 cells x10(6)/femur and the frequency of the CFU-F per femur by similar to 70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells x 10(6)/femur and 230 CFU-F/femur). Conclusion Although the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.
PB  - Medicinska Naklada, Zagreb
T2  - Croatian Medical Journal
T1  - Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector
EP  - 49
IS  - 1
SP  - 45
VL  - 55
DO  - 10.3325/cmj.2014.55.45
ER  - 

@article{
author = "Okić Đorđević, Ivana and Trivanović, Drenka and Jovanović, Miloš and Ignjatović, Marija and Secerov, Bojana and Mojović, Miloš and Bugarski, Diana and Bacić, Goran and Anđus, Pavle R.",
year = "2014",
abstract = "Aim To investigate the survival of laboratory rats after irradiation and to study the cellularity of their bone marrow and the multipotential mesenchymal stem cells (BM-MSCs) in groups treated with or without a new thiol-based radioprotector (GM2011) Methods Animals were irradiated by a Cobalt gamma source at 6.7 Gy. Treated animals were given i.p. GM2011 30 minutes before and 3 and 7 hours after irradiation. Controls consisted of sham irradiated animals without treatment and animals treated without irradiation. After 30 days post-irradiation, animals were sacrificed and bone marrow cells were prepared from isolated femurs. A colony forming unit-fibroblast (CFU-F) assay was performed to obtain the number of BM-MSCs. Results In the treated group, 87% of animals survived, compared to only 30% in the non-treated irradiated group. Irradiation induced significant changes in the bone marrow of the treated rats (total bone marrow cellularity was reduced by similar to 60% - from 63 to 28 cells x10(6)/femur and the frequency of the CFU-F per femur by similar to 70% - from 357 to 97), however GL2011 almost completely prevented the suppressive effect observed on day 30 post-irradiation (71 cells x 10(6)/femur and 230 CFU-F/femur). Conclusion Although the irradiation dosage was relatively high, GL2011 acted as a very effective new radioprotector. The recovery of the BN-MSCs and their counts support the effectiveness of the studied radioprotector.",
publisher = "Medicinska Naklada, Zagreb",
journal = "Croatian Medical Journal",
title = "Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector",
pages = "49-45",
number = "1",
volume = "55",
doi = "10.3325/cmj.2014.55.45"
}

Okić Đorđević, I., Trivanović, D., Jovanović, M., Ignjatović, M., Secerov, B., Mojović, M., Bugarski, D., Bacić, G.,& Anđus, P. R.. (2014). Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector. in Croatian Medical Journal
Medicinska Naklada, Zagreb., 55(1), 45-49.
https://doi.org/10.3325/cmj.2014.55.45

Okić Đorđević I, Trivanović D, Jovanović M, Ignjatović M, Secerov B, Mojović M, Bugarski D, Bacić G, Anđus PR. Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector. in Croatian Medical Journal. 2014;55(1):45-49.
doi:10.3325/cmj.2014.55.45 .

Okić Đorđević, Ivana, Trivanović, Drenka, Jovanović, Miloš, Ignjatović, Marija, Secerov, Bojana, Mojović, Miloš, Bugarski, Diana, Bacić, Goran, Anđus, Pavle R., "Increased survival after irradiation followed by regeneration of bone marrow stromal cells with a novel thiol-based radioprotector" in Croatian Medical Journal, 55, no. 1 (2014):45-49,
https://doi.org/10.3325/cmj.2014.55.45 . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Jauković, Aleksandra
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/599
AB  - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Mesenchymal stem cells isolated from human periodontal ligament
EP  - 271
IS  - 1
SP  - 261
VL  - 66
DO  - 10.2298/ABS1401261M
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okić Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cells isolated from human periodontal ligament",
pages = "271-261",
number = "1",
volume = "66",
doi = "10.2298/ABS1401261M"
}

Miletić, M., Mojsilović, S., Okić Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271.
https://doi.org/10.2298/ABS1401261M

Miletić M, Mojsilović S, Okić Đorđević I, Kukolj T, Jauković A, Santibanez JF, Jovčić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271.
doi:10.2298/ABS1401261M .

Miletić, Maja, Mojsilović, S., Okić Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271,
https://doi.org/10.2298/ABS1401261M . .