TY  - JOUR
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Bogosavljević, Nikola
AU  - Jurišić, Vladimir
AU  - Jauković, Aleksandra
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1460
AB  - Among multiple hemostasis components, platelets hyperactivity plays major roles in cancer progression by providing surface and internal components for intercellular crosstalk as well as by behaving like immune cells. Since platelets participate and regulate immunity in homeostatic and disease states, we assumed that revealing platelets profile might help in conceiving novel anti-cancer immune-based strategies. The goal of this review is to compile and discuss the most recent reports on the nature of cancer-associated platelets and their interference with immunotherapy. An increasing number of studies have emphasized active communication between cancer cells and platelets, with platelets promoting cancer cell survival, growth, and metastasis. The anti-cancer potential of platelet-directed therapy has been intensively investigated, and anti-platelet agents may prevent cancer progression and improve the survival of cancer patients. Platelets can (i) reduce antitumor activity; (ii) support immunoregulatory cells and factors generation; (iii) underpin metastasis and, (iv) interfere with immunotherapy by expressing ligands of immune checkpoint receptors. Mediators produced by tumor cell-induced platelet activation support vein thrombosis, constrain anti-tumor T- and natural killer cell response, while contributing to extravasation of tumor cells, metastatic potential, and neovascularization within the tumor. Recent studies showed that attenuation of immunothrombosis, modulation of platelets and their factors have a good perspective in immunotherapy optimization. Particularly, blockade of intra-tumoral platelet-associated programmed death-ligand 1 might promote anti-tumor T cell-induced cytotoxicity. Collectively, these findings suggest that platelets might represent the source of relevant cancer staging biomarkers, as well as promising targets and carriers in immunotherapeutic approaches for combating cancer.
PB  - Neoplasia Press, Inc.
T2  - Translational Oncology
T2  - Translational OncologyTranslational Oncology
T1  - Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development
SP  - 101871
VL  - 40
DO  - 10.1016/j.tranon.2023.101871
ER  - 

@article{
author = "Trivanović, Drenka and Mojsilović, Slavko and Bogosavljević, Nikola and Jurišić, Vladimir and Jauković, Aleksandra",
year = "2024",
abstract = "Among multiple hemostasis components, platelets hyperactivity plays major roles in cancer progression by providing surface and internal components for intercellular crosstalk as well as by behaving like immune cells. Since platelets participate and regulate immunity in homeostatic and disease states, we assumed that revealing platelets profile might help in conceiving novel anti-cancer immune-based strategies. The goal of this review is to compile and discuss the most recent reports on the nature of cancer-associated platelets and their interference with immunotherapy. An increasing number of studies have emphasized active communication between cancer cells and platelets, with platelets promoting cancer cell survival, growth, and metastasis. The anti-cancer potential of platelet-directed therapy has been intensively investigated, and anti-platelet agents may prevent cancer progression and improve the survival of cancer patients. Platelets can (i) reduce antitumor activity; (ii) support immunoregulatory cells and factors generation; (iii) underpin metastasis and, (iv) interfere with immunotherapy by expressing ligands of immune checkpoint receptors. Mediators produced by tumor cell-induced platelet activation support vein thrombosis, constrain anti-tumor T- and natural killer cell response, while contributing to extravasation of tumor cells, metastatic potential, and neovascularization within the tumor. Recent studies showed that attenuation of immunothrombosis, modulation of platelets and their factors have a good perspective in immunotherapy optimization. Particularly, blockade of intra-tumoral platelet-associated programmed death-ligand 1 might promote anti-tumor T cell-induced cytotoxicity. Collectively, these findings suggest that platelets might represent the source of relevant cancer staging biomarkers, as well as promising targets and carriers in immunotherapeutic approaches for combating cancer.",
publisher = "Neoplasia Press, Inc.",
journal = "Translational Oncology, Translational OncologyTranslational Oncology",
title = "Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development",
pages = "101871",
volume = "40",
doi = "10.1016/j.tranon.2023.101871"
}

Trivanović, D., Mojsilović, S., Bogosavljević, N., Jurišić, V.,& Jauković, A.. (2024). Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development. in Translational Oncology
Neoplasia Press, Inc.., 40, 101871.
https://doi.org/10.1016/j.tranon.2023.101871

Trivanović D, Mojsilović S, Bogosavljević N, Jurišić V, Jauković A. Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development. in Translational Oncology. 2024;40:101871.
doi:10.1016/j.tranon.2023.101871 .

Trivanović, Drenka, Mojsilović, Slavko, Bogosavljević, Nikola, Jurišić, Vladimir, Jauković, Aleksandra, "Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development" in Translational Oncology, 40 (2024):101871,
https://doi.org/10.1016/j.tranon.2023.101871 . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Brković, Božidar
AU  - Andrić, Miroslav
AU  - Prokić, Bogomir Bolka
AU  - Danilović, Vesna
AU  - Milutinović-Smiljanić, Sanja
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1464
AB  - In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.
T2  - Applied Sciences
T2  - Applied Sciences
T1  - Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment
IS  - 1
SP  - 16
VL  - 14
DO  - 10.3390/app14010016
ER  - 

@article{
author = "Miletić, Maja and Puač, Nevena and Škoro, Nikola and Brković, Božidar and Andrić, Miroslav and Prokić, Bogomir Bolka and Danilović, Vesna and Milutinović-Smiljanić, Sanja and Mitrović-Ajtić, Olivera and Mojsilović, Slavko",
year = "2024",
abstract = "In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.",
journal = "Applied Sciences, Applied Sciences",
title = "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment",
number = "1",
pages = "16",
volume = "14",
doi = "10.3390/app14010016"
}

Miletić, M., Puač, N., Škoro, N., Brković, B., Andrić, M., Prokić, B. B., Danilović, V., Milutinović-Smiljanić, S., Mitrović-Ajtić, O.,& Mojsilović, S.. (2024). Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences, 14(1), 16.
https://doi.org/10.3390/app14010016

Miletić M, Puač N, Škoro N, Brković B, Andrić M, Prokić BB, Danilović V, Milutinović-Smiljanić S, Mitrović-Ajtić O, Mojsilović S. Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences. 2024;14(1):16.
doi:10.3390/app14010016 .

Miletić, Maja, Puač, Nevena, Škoro, Nikola, Brković, Božidar, Andrić, Miroslav, Prokić, Bogomir Bolka, Danilović, Vesna, Milutinović-Smiljanić, Sanja, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment" in Applied Sciences, 14, no. 1 (2024):16,
https://doi.org/10.3390/app14010016 . .

TY  - JOUR
AU  - Pires, Ana Salomé
AU  - Bollini, Sveva
AU  - Botelho, Maria Filomena
AU  - Lang-Olip, Ingrid
AU  - Ponsaerts, Peter
AU  - Balbi, Carolina
AU  - Lange-Consiglio, Anna
AU  - Fénelon, Mathilde
AU  - Mojsilović, Slavko
AU  - Berishvili, Ekaterine
AU  - Cremonesi, Fausto
AU  - Gazouli, Maria
AU  - Bugarski, Diana
AU  - Gellhaus, Alexandra
AU  - Kerdjoudj, Halima
AU  - Schoeberlein, Andreina
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1297
AB  - The last 18 years have brought an increasing interest in the therapeutic use of perinatal derivatives (PnD). Preclinical studies used to assess the potential of PnD therapy include a broad range of study designs. The COST SPRINT Action (CA17116) aims to provide systematic and comprehensive reviews of preclinical studies for the understanding of the therapeutic potential and mechanisms of PnD in diseases and injuries that benefit from PnD therapy. Here we describe the publication search and data mining, extraction, and synthesis strategies employed to collect and prepare the published data selected for meta-analyses and reviews of the efficacy of PnD therapies for different diseases and injuries. A coordinated effort was made to prepare the data suitable to make statements for the treatment efficacy of the different types of PnD, routes, time points, and frequencies of administration, and the dosage based on clinically relevant effects resulting in clear increase, recovery or amelioration of the specific tissue or organ function. According to recently proposed guidelines, the harmonization of the nomenclature of PnD types will allow for the assessment of the most efficient treatments in various disease models. Experts within the COST SPRINT Action (CA17116), together with external collaborators, are doing the meta-analyses and reviews using the data prepared with the strategies presented here in the relevant disease or research fields. Our final aim is to provide standards to assess the safety and clinical benefit of PnD and to minimize redundancy in the use of animal models following the 3R principles for animal experimentation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Methods and Protocols
T1  - Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives
IS  - 3
SP  - 45
VL  - 6
DO  - 10.3390/mps6030045
ER  - 

@article{
author = "Pires, Ana Salomé and Bollini, Sveva and Botelho, Maria Filomena and Lang-Olip, Ingrid and Ponsaerts, Peter and Balbi, Carolina and Lange-Consiglio, Anna and Fénelon, Mathilde and Mojsilović, Slavko and Berishvili, Ekaterine and Cremonesi, Fausto and Gazouli, Maria and Bugarski, Diana and Gellhaus, Alexandra and Kerdjoudj, Halima and Schoeberlein, Andreina",
year = "2023",
abstract = "The last 18 years have brought an increasing interest in the therapeutic use of perinatal derivatives (PnD). Preclinical studies used to assess the potential of PnD therapy include a broad range of study designs. The COST SPRINT Action (CA17116) aims to provide systematic and comprehensive reviews of preclinical studies for the understanding of the therapeutic potential and mechanisms of PnD in diseases and injuries that benefit from PnD therapy. Here we describe the publication search and data mining, extraction, and synthesis strategies employed to collect and prepare the published data selected for meta-analyses and reviews of the efficacy of PnD therapies for different diseases and injuries. A coordinated effort was made to prepare the data suitable to make statements for the treatment efficacy of the different types of PnD, routes, time points, and frequencies of administration, and the dosage based on clinically relevant effects resulting in clear increase, recovery or amelioration of the specific tissue or organ function. According to recently proposed guidelines, the harmonization of the nomenclature of PnD types will allow for the assessment of the most efficient treatments in various disease models. Experts within the COST SPRINT Action (CA17116), together with external collaborators, are doing the meta-analyses and reviews using the data prepared with the strategies presented here in the relevant disease or research fields. Our final aim is to provide standards to assess the safety and clinical benefit of PnD and to minimize redundancy in the use of animal models following the 3R principles for animal experimentation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Methods and Protocols",
title = "Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives",
number = "3",
pages = "45",
volume = "6",
doi = "10.3390/mps6030045"
}

Pires, A. S., Bollini, S., Botelho, M. F., Lang-Olip, I., Ponsaerts, P., Balbi, C., Lange-Consiglio, A., Fénelon, M., Mojsilović, S., Berishvili, E., Cremonesi, F., Gazouli, M., Bugarski, D., Gellhaus, A., Kerdjoudj, H.,& Schoeberlein, A.. (2023). Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives. in Methods and Protocols
Multidisciplinary Digital Publishing Institute (MDPI)., 6(3), 45.
https://doi.org/10.3390/mps6030045

Pires AS, Bollini S, Botelho MF, Lang-Olip I, Ponsaerts P, Balbi C, Lange-Consiglio A, Fénelon M, Mojsilović S, Berishvili E, Cremonesi F, Gazouli M, Bugarski D, Gellhaus A, Kerdjoudj H, Schoeberlein A. Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives. in Methods and Protocols. 2023;6(3):45.
doi:10.3390/mps6030045 .

Pires, Ana Salomé, Bollini, Sveva, Botelho, Maria Filomena, Lang-Olip, Ingrid, Ponsaerts, Peter, Balbi, Carolina, Lange-Consiglio, Anna, Fénelon, Mathilde, Mojsilović, Slavko, Berishvili, Ekaterine, Cremonesi, Fausto, Gazouli, Maria, Bugarski, Diana, Gellhaus, Alexandra, Kerdjoudj, Halima, Schoeberlein, Andreina, "Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives" in Methods and Protocols, 6, no. 3 (2023):45,
https://doi.org/10.3390/mps6030045 . .

TY  - JOUR
AU  - Momčilović, Sanja
AU  - Bogdanović, Andrija
AU  - Milošević, Maja
AU  - Mojsilović, Slavko
AU  - Marković, Dragana
AU  - Kočović, Dušica M.
AU  - Vignjević-Petrinović, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1329
AB  - Psychological stress is a significant contributor to various chronic diseases and affects multiple physiological processes including erythropoiesis. This study aimed to examine the tissue-specific contributions of macrophages and extracellular ATP, as a signal of disturbed tissue homeostasis, to erythropoiesis under conditions of repeated psychological stress. Adult male BALB/c mice were subjected to 2 h daily restraint stress for seven consecutive days. Clodronate-liposomes were used to deplete resident macrophages from the bone marrow and spleen two days prior to the first restraint procedure, as well as newly recruited macrophages, every third day for the duration of the experiment. Repeated stress induced a considerable increase in the number of erythroid progenitor cells as well as in the percentage of CD71+/Ter119+ and CD71−/Ter119+ cells in the bone marrow and spleen. Macrophage depletion completely abolished the stimulative effect of repeated stress on immature erythroid cells, and prevented stress-induced increases in ATP levels, P2X7 receptor (P2X7R) expression, and ectonucleotidase CD39 activity and expression in the bone marrow and spleen. The obtained results demonstrate the stimulative effects of repeated stress on erythroid cells, extracellular ATP levels, P2X7R expression, CD39 activity and expression within the bone marrow and spleen, as well as the essential role of macrophages in stress-induced changes.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress
IS  - 14
SP  - 11373
VL  - 24
DO  - 10.3390/ijms241411373
ER  - 

@article{
author = "Momčilović, Sanja and Bogdanović, Andrija and Milošević, Maja and Mojsilović, Slavko and Marković, Dragana and Kočović, Dušica M. and Vignjević-Petrinović, Sanja",
year = "2023",
abstract = "Psychological stress is a significant contributor to various chronic diseases and affects multiple physiological processes including erythropoiesis. This study aimed to examine the tissue-specific contributions of macrophages and extracellular ATP, as a signal of disturbed tissue homeostasis, to erythropoiesis under conditions of repeated psychological stress. Adult male BALB/c mice were subjected to 2 h daily restraint stress for seven consecutive days. Clodronate-liposomes were used to deplete resident macrophages from the bone marrow and spleen two days prior to the first restraint procedure, as well as newly recruited macrophages, every third day for the duration of the experiment. Repeated stress induced a considerable increase in the number of erythroid progenitor cells as well as in the percentage of CD71+/Ter119+ and CD71−/Ter119+ cells in the bone marrow and spleen. Macrophage depletion completely abolished the stimulative effect of repeated stress on immature erythroid cells, and prevented stress-induced increases in ATP levels, P2X7 receptor (P2X7R) expression, and ectonucleotidase CD39 activity and expression in the bone marrow and spleen. The obtained results demonstrate the stimulative effects of repeated stress on erythroid cells, extracellular ATP levels, P2X7R expression, CD39 activity and expression within the bone marrow and spleen, as well as the essential role of macrophages in stress-induced changes.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress",
number = "14",
pages = "11373",
volume = "24",
doi = "10.3390/ijms241411373"
}

Momčilović, S., Bogdanović, A., Milošević, M., Mojsilović, S., Marković, D., Kočović, D. M.,& Vignjević-Petrinović, S.. (2023). Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress. in International Journal of Molecular Sciences
Multidisciplinary Digital Publishing Institute (MDPI)., 24(14), 11373.
https://doi.org/10.3390/ijms241411373

Momčilović S, Bogdanović A, Milošević M, Mojsilović S, Marković D, Kočović DM, Vignjević-Petrinović S. Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress. in International Journal of Molecular Sciences. 2023;24(14):11373.
doi:10.3390/ijms241411373 .

Momčilović, Sanja, Bogdanović, Andrija, Milošević, Maja, Mojsilović, Slavko, Marković, Dragana, Kočović, Dušica M., Vignjević-Petrinović, Sanja, "Macrophages Provide Essential Support for Erythropoiesis, and Extracellular ATP Contributes to a Erythropoiesis-Supportive Microenvironment during Repeated Psychological Stress" in International Journal of Molecular Sciences, 24, no. 14 (2023):11373,
https://doi.org/10.3390/ijms241411373 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1346
AB  - Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T2  - Biomolecules
T1  - The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis
IS  - 10
SP  - 1437
VL  - 13
DO  - 10.3390/biom13101437
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules, Biomolecules",
title = "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis",
number = "10",
pages = "1437",
volume = "13",
doi = "10.3390/biom13101437"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 13(10), 1437.
https://doi.org/10.3390/biom13101437

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules. 2023;13(10):1437.
doi:10.3390/biom13101437 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis" in Biomolecules, 13, no. 10 (2023):1437,
https://doi.org/10.3390/biom13101437 . .

TY  - JOUR
AU  - Petrović, Anđelija
AU  - Čanković, Miloš
AU  - Avramov, Miloš
AU  - Popović, Željko D.
AU  - Janković, Srđa
AU  - Mojsilović, Slavko
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1360
AB  - Background and Objectives: Oral squamous cell carcinoma (OSCC) accounts for about 95% of oral cancers. It represents a serious public health problem due to the high degree of morbidity and mortality, as well as multifactorial etiology. Human papillomavirus (HPV) infection is a well-documented risk factor for oropharyngeal carcinoma, but its role in oral carcinogenesis is still debatable. Our aim was to investigate the differences in the prevalence of high-risk HPV genotypes (HR-HPV) in patients with OSCC and oral potentially malignant disorders (OPMD) from that of healthy subjects. Materials and Methods: A total of 90 subjects were included in the cross-sectional study and divided into three groups of 30 patients each: (1) patients with OSCC, (2) patients with OPMD, and (3) healthy subjects. We examined the presence of 12 HR-HPV genotypes in the obtained biological material (oral swabs) using real-time PCR. Results: One or more of the 12 tested HR-HPV genotypes were detected in 5/30 patients with OSCC and 2/30 with OPMD, whereas no healthy subjects were positive for any of the tested genotypes. There was a statistically significant difference in nodal involvement between HPV-positive and HPV-negative patients with OSCC. Conclusions: Oral HR-HPV was detected in patients with oral premalignant and malignant lesions but not in healthy individuals, suggesting a possible role in oral carcinogenesis. Broad HR-HPV panel testing could increase the sensitivity of risk assessment and screening for OSCC.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Medicina
T2  - Medicina
T1  - High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study
IS  - 10
SP  - 1843
VL  - 59
DO  - 10.3390/medicina59101843
ER  - 

@article{
author = "Petrović, Anđelija and Čanković, Miloš and Avramov, Miloš and Popović, Željko D. and Janković, Srđa and Mojsilović, Slavko",
year = "2023",
abstract = "Background and Objectives: Oral squamous cell carcinoma (OSCC) accounts for about 95% of oral cancers. It represents a serious public health problem due to the high degree of morbidity and mortality, as well as multifactorial etiology. Human papillomavirus (HPV) infection is a well-documented risk factor for oropharyngeal carcinoma, but its role in oral carcinogenesis is still debatable. Our aim was to investigate the differences in the prevalence of high-risk HPV genotypes (HR-HPV) in patients with OSCC and oral potentially malignant disorders (OPMD) from that of healthy subjects. Materials and Methods: A total of 90 subjects were included in the cross-sectional study and divided into three groups of 30 patients each: (1) patients with OSCC, (2) patients with OPMD, and (3) healthy subjects. We examined the presence of 12 HR-HPV genotypes in the obtained biological material (oral swabs) using real-time PCR. Results: One or more of the 12 tested HR-HPV genotypes were detected in 5/30 patients with OSCC and 2/30 with OPMD, whereas no healthy subjects were positive for any of the tested genotypes. There was a statistically significant difference in nodal involvement between HPV-positive and HPV-negative patients with OSCC. Conclusions: Oral HR-HPV was detected in patients with oral premalignant and malignant lesions but not in healthy individuals, suggesting a possible role in oral carcinogenesis. Broad HR-HPV panel testing could increase the sensitivity of risk assessment and screening for OSCC.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Medicina, Medicina",
title = "High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study",
number = "10",
pages = "1843",
volume = "59",
doi = "10.3390/medicina59101843"
}

Petrović, A., Čanković, M., Avramov, M., Popović, Ž. D., Janković, S.,& Mojsilović, S.. (2023). High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study. in Medicina
Multidisciplinary Digital Publishing Institute (MDPI)., 59(10), 1843.
https://doi.org/10.3390/medicina59101843

Petrović A, Čanković M, Avramov M, Popović ŽD, Janković S, Mojsilović S. High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study. in Medicina. 2023;59(10):1843.
doi:10.3390/medicina59101843 .

Petrović, Anđelija, Čanković, Miloš, Avramov, Miloš, Popović, Željko D., Janković, Srđa, Mojsilović, Slavko, "High-Risk Human Papillomavirus in Patients with Oral Carcinoma and Oral Potentially Malignant Disorders in Serbia—A Pilot Study" in Medicina, 59, no. 10 (2023):1843,
https://doi.org/10.3390/medicina59101843 . .

TY  - CHAP
AU  - Kapor, Sunčica
AU  - Momčilović, Sanja
AU  - Kapor, Slobodan
AU  - Mojsilović, Slavko
AU  - Radojković, Milica
AU  - Apostolović, Milica
AU  - Filipović, Branka
AU  - Gotić, Mirjana
AU  - Čokić, Vladan
AU  - Santibanez, Juan F.
AU  - Simon, Felipe
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1391
AB  - The Philadelphia-negative myeloproliferative neoplasms (MPNs), defined as clonal disorders of the hematopoietic stem cells, are characterized by the proliferation of mature myeloid cells in the bone marrow and a chronic inflammatory status impacting the initiation, progression, and symptomatology of the malignancies. There are three main entities defined as essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF), and genetically classified by JAK2V617F, CALR, or MPL mutations. In MPNs, due to the overproduction of inflammatory cytokines by the neoplastic cells and non-transformed immune cells, chronic inflammation may provoke the generation and expansion of myeloid-derived suppressors cells (MDSCs) that highly influence the adaptive immune response. Although peripheral blood MDSC levels are elevated, their frequency in the bone marrow of MPNs patients is not well elucidated yet. Our results indicated increased levels of total (T)-MDSCs (CD33+HLA-DR−/low) and polymorphonuclear (PMN)-MDSCs (CD33+/HLA-DRlow/CD15+/CD14−) in the bone marrow and peripheral blood of all three types of MPNs malignancies. However, these bone marrow MDSCs-increased frequencies did not correlate with the clinical parameters, such as hepatomegaly, leukocytes, hemoglobin, or platelet levels, or with JAK2 and CALR mutations. Besides, bone marrow MDSCs, from ET, PV, and PMF patients, exhibited immunosuppressive function, determined as T-cell proliferation inhibition. Notably, the highest T-MDSCs and PMN-MDSC levels were found in PMF samples, and the increased MDSCs frequency strongly correlated with the degree of myelofibrosis. Thus, these data together indicate that the immunosuppressive MDSCs population is increased in the bone marrow of MPNs patients and may be implicated in generating a fibrotic microenvironment.
PB  - Springer Nature
T2  - Advances in Molecular Pathology
T1  - Increase in Frequency of Myeloid-Derived Suppressor Cells in the Bone Marrow of Myeloproliferative Neoplasm: Potential Implications in Myelofibrosis
EP  - 290
SP  - 273
VL  - 1408
DO  - 10.1007/978-3-031-26163-3_15
ER  - 

@inbook{
author = "Kapor, Sunčica and Momčilović, Sanja and Kapor, Slobodan and Mojsilović, Slavko and Radojković, Milica and Apostolović, Milica and Filipović, Branka and Gotić, Mirjana and Čokić, Vladan and Santibanez, Juan F. and Simon, Felipe",
year = "2023",
abstract = "The Philadelphia-negative myeloproliferative neoplasms (MPNs), defined as clonal disorders of the hematopoietic stem cells, are characterized by the proliferation of mature myeloid cells in the bone marrow and a chronic inflammatory status impacting the initiation, progression, and symptomatology of the malignancies. There are three main entities defined as essential thrombocythemia (ET), polycythemia vera (PV), and primary myelofibrosis (PMF), and genetically classified by JAK2V617F, CALR, or MPL mutations. In MPNs, due to the overproduction of inflammatory cytokines by the neoplastic cells and non-transformed immune cells, chronic inflammation may provoke the generation and expansion of myeloid-derived suppressors cells (MDSCs) that highly influence the adaptive immune response. Although peripheral blood MDSC levels are elevated, their frequency in the bone marrow of MPNs patients is not well elucidated yet. Our results indicated increased levels of total (T)-MDSCs (CD33+HLA-DR−/low) and polymorphonuclear (PMN)-MDSCs (CD33+/HLA-DRlow/CD15+/CD14−) in the bone marrow and peripheral blood of all three types of MPNs malignancies. However, these bone marrow MDSCs-increased frequencies did not correlate with the clinical parameters, such as hepatomegaly, leukocytes, hemoglobin, or platelet levels, or with JAK2 and CALR mutations. Besides, bone marrow MDSCs, from ET, PV, and PMF patients, exhibited immunosuppressive function, determined as T-cell proliferation inhibition. Notably, the highest T-MDSCs and PMN-MDSC levels were found in PMF samples, and the increased MDSCs frequency strongly correlated with the degree of myelofibrosis. Thus, these data together indicate that the immunosuppressive MDSCs population is increased in the bone marrow of MPNs patients and may be implicated in generating a fibrotic microenvironment.",
publisher = "Springer Nature",
journal = "Advances in Molecular Pathology",
booktitle = "Increase in Frequency of Myeloid-Derived Suppressor Cells in the Bone Marrow of Myeloproliferative Neoplasm: Potential Implications in Myelofibrosis",
pages = "290-273",
volume = "1408",
doi = "10.1007/978-3-031-26163-3_15"
}

Kapor, S., Momčilović, S., Kapor, S., Mojsilović, S., Radojković, M., Apostolović, M., Filipović, B., Gotić, M., Čokić, V., Santibanez, J. F.,& Simon, F.. (2023). Increase in Frequency of Myeloid-Derived Suppressor Cells in the Bone Marrow of Myeloproliferative Neoplasm: Potential Implications in Myelofibrosis. in Advances in Molecular Pathology
Springer Nature., 1408, 273-290.
https://doi.org/10.1007/978-3-031-26163-3_15

Kapor S, Momčilović S, Kapor S, Mojsilović S, Radojković M, Apostolović M, Filipović B, Gotić M, Čokić V, Santibanez JF, Simon F. Increase in Frequency of Myeloid-Derived Suppressor Cells in the Bone Marrow of Myeloproliferative Neoplasm: Potential Implications in Myelofibrosis. in Advances in Molecular Pathology. 2023;1408:273-290.
doi:10.1007/978-3-031-26163-3_15 .

Kapor, Sunčica, Momčilović, Sanja, Kapor, Slobodan, Mojsilović, Slavko, Radojković, Milica, Apostolović, Milica, Filipović, Branka, Gotić, Mirjana, Čokić, Vladan, Santibanez, Juan F., Simon, Felipe, "Increase in Frequency of Myeloid-Derived Suppressor Cells in the Bone Marrow of Myeloproliferative Neoplasm: Potential Implications in Myelofibrosis" in Advances in Molecular Pathology, 1408 (2023):273-290,
https://doi.org/10.1007/978-3-031-26163-3_15 . .

TY  - CONF
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1406
AB  - Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.
PB  - Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije
C3  - Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
T1  - Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1406
ER  - 

@conference{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.",
publisher = "Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije",
journal = "Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka",
title = "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1406"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije..
https://hdl.handle.net/21.15107/rcub_rimi_1406

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka. 2023;.
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma" in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka (2023),
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Vujačić, Marko
AU  - Arsić, Aleksandra
AU  - Bogosavljević, Nikola
AU  - Kovačić, Marijana
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Baščarević, Zoran
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1408
AB  - Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients
IS  - 3
SP  - P140
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1408
ER  - 

@conference{
author = "Trivanović, Drenka and Vujačić, Marko and Arsić, Aleksandra and Bogosavljević, Nikola and Kovačić, Marijana and Drvenica, Ivana and Mojsilović, Slavko and Baščarević, Zoran and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients",
number = "3",
pages = "P140",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1408"
}

Trivanović, D., Vujačić, M., Arsić, A., Bogosavljević, N., Kovačić, M., Drvenica, I., Mojsilović, S., Baščarević, Z., Bugarski, D.,& Jauković, A.. (2023). Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408

Trivanović D, Vujačić M, Arsić A, Bogosavljević N, Kovačić M, Drvenica I, Mojsilović S, Baščarević Z, Bugarski D, Jauković A. Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

Trivanović, Drenka, Vujačić, Marko, Arsić, Aleksandra, Bogosavljević, Nikola, Kovačić, Marijana, Drvenica, Ivana, Mojsilović, Slavko, Baščarević, Zoran, Bugarski, Diana, Jauković, Aleksandra, "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P140,
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

TY  - CONF
AU  - Miletić, Maja
AU  - Pavlović, Ognjan
AU  - Škoro, Nikola
AU  - Lazarević, Miloš
AU  - Jakovljević, Aleksandar
AU  - Mojsilović, Slavko
AU  - Puač, Nevena
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1431
AB  - Oral squamous cell carcinoma (OSCC) is one of the most malignant neoplasms of the oral cavity, with a high mortality rate. Since the long-term survival rate of patients diagnosed with OSCC has remained unchanged over the past several decades, it is of utmost importance to discover new treatment modalities or enhance existing ones [1]. Since we previously demonstrated the antitumor efficacy of cold atmospheric pressure plasma (CAP) on OSCC cell lines in a two-dimensional (2D) monolayer cell model, we moved further and tried to explain the mechanism of these cytotoxic events as well as analyze the effect of plasma-activated medium (PAM) in combination with chemotherapy as a gold standard in carcinoma treatment. The modified plasma needle operating at 13.56MHz with He as working gas was used for PAM generation with the distance of 3 mm between liquid surface and the tube and applying different exposure intervals [2]. OSCC cell line (SCC25) was cultivated in 2D or 3D culture systems when regular culture medium was changed for PAM for 24 h before performing assays. PAM treatment showed cytotoxic effects on 2D-cultured OSCC by inducing apoptotic cell death through the activation of the intrinsic caspase pathway. To analyze the combined effect of cisplatin and PAM we used a 3D cell culture approach with OSCC spheroids, as this method reflects more closely the in vivo cellular response to chemotherapeutics [3]. When PAM was combined with the increasing concentrations of cisplatin, the results showed an almost linear dose dependent decrease in OSCC spheroid viability. These arepromising and encouraging results for a potential application of CAP in the treatment of oral carcinoma. By combining the effects of chemotherapeutics with PAM, we developed a new prospect for a possible cancer treatment in which the same or even better antitumor effects could be achieved with lower doses of cytotoxic drugs. Consequently, it means that with lower doses of chemotherapeutics, we could minimize potential side effects associated with the high-dose usage of cytostatics and improve the quality of life for patients with these malignancies.
C3  - 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy
T1  - Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1431
ER  - 

@conference{
author = "Miletić, Maja and Pavlović, Ognjan and Škoro, Nikola and Lazarević, Miloš and Jakovljević, Aleksandar and Mojsilović, Slavko and Puač, Nevena",
year = "2023",
abstract = "Oral squamous cell carcinoma (OSCC) is one of the most malignant neoplasms of the oral cavity, with a high mortality rate. Since the long-term survival rate of patients diagnosed with OSCC has remained unchanged over the past several decades, it is of utmost importance to discover new treatment modalities or enhance existing ones [1]. Since we previously demonstrated the antitumor efficacy of cold atmospheric pressure plasma (CAP) on OSCC cell lines in a two-dimensional (2D) monolayer cell model, we moved further and tried to explain the mechanism of these cytotoxic events as well as analyze the effect of plasma-activated medium (PAM) in combination with chemotherapy as a gold standard in carcinoma treatment. The modified plasma needle operating at 13.56MHz with He as working gas was used for PAM generation with the distance of 3 mm between liquid surface and the tube and applying different exposure intervals [2]. OSCC cell line (SCC25) was cultivated in 2D or 3D culture systems when regular culture medium was changed for PAM for 24 h before performing assays. PAM treatment showed cytotoxic effects on 2D-cultured OSCC by inducing apoptotic cell death through the activation of the intrinsic caspase pathway. To analyze the combined effect of cisplatin and PAM we used a 3D cell culture approach with OSCC spheroids, as this method reflects more closely the in vivo cellular response to chemotherapeutics [3]. When PAM was combined with the increasing concentrations of cisplatin, the results showed an almost linear dose dependent decrease in OSCC spheroid viability. These arepromising and encouraging results for a potential application of CAP in the treatment of oral carcinoma. By combining the effects of chemotherapeutics with PAM, we developed a new prospect for a possible cancer treatment in which the same or even better antitumor effects could be achieved with lower doses of cytotoxic drugs. Consequently, it means that with lower doses of chemotherapeutics, we could minimize potential side effects associated with the high-dose usage of cytostatics and improve the quality of life for patients with these malignancies.",
journal = "2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy",
title = "Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1431"
}

Miletić, M., Pavlović, O., Škoro, N., Lazarević, M., Jakovljević, A., Mojsilović, S.,& Puač, N.. (2023). Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models. in 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy, 82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1431

Miletić M, Pavlović O, Škoro N, Lazarević M, Jakovljević A, Mojsilović S, Puač N. Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models. in 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy. 2023;:82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1431 .

Miletić, Maja, Pavlović, Ognjan, Škoro, Nikola, Lazarević, Miloš, Jakovljević, Aleksandar, Mojsilović, Slavko, Puač, Nevena, "Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models" in 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy (2023):82-82,
https://hdl.handle.net/21.15107/rcub_rimi_1431 .

TY  - JOUR
AU  - Mojsilović, Slavko
AU  - Mojsilović, Sonja
AU  - Bjelica, Sunčica
AU  - Santibanez, Juan F.
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1180
AB  - Transforming growth factor-beta1 (TGF-beta 1) plays a crucial role in tumor progression. It can inhibit early cancer stages but promotes tumor growth and development at the late stages of tumorigenesis. TGF-beta 1 has a potent immunosuppressive function within the tumor microenvironment that largely contributes to tumor cells' immune escape and reduction in cancer immunotherapy responses. Likewise, myeloid-derived suppressor cells (MDSCs) have been postulated as leading tumor promoters and a hallmark of cancer immune evasion mechanisms. This review attempts to analyze the prominent roles of both TGF-beta 1 and MDSCs and their interplay in cancer immunity. Furthermore, therapies against either TGF-beta 1 or MDSCs, and their potential synergistic combination with immunotherapies are discussed. Simultaneous TGF-beta 1 and MDSCs inhibition suggest a potential improvement in immunotherapy or subverted tumor immune resistance.
PB  - Wiley
T2  - Developmental Dinamics
T1  - Transforming growth factor-beta1 and myeloid-derived suppressor cells: A cancerous partnership
EP  - 124
IS  - 1
SP  - 105
DO  - 10.1002/dvdy.339
ER  - 

@article{
author = "Mojsilović, Slavko and Mojsilović, Sonja and Bjelica, Sunčica and Santibanez, Juan F.",
year = "2022",
abstract = "Transforming growth factor-beta1 (TGF-beta 1) plays a crucial role in tumor progression. It can inhibit early cancer stages but promotes tumor growth and development at the late stages of tumorigenesis. TGF-beta 1 has a potent immunosuppressive function within the tumor microenvironment that largely contributes to tumor cells' immune escape and reduction in cancer immunotherapy responses. Likewise, myeloid-derived suppressor cells (MDSCs) have been postulated as leading tumor promoters and a hallmark of cancer immune evasion mechanisms. This review attempts to analyze the prominent roles of both TGF-beta 1 and MDSCs and their interplay in cancer immunity. Furthermore, therapies against either TGF-beta 1 or MDSCs, and their potential synergistic combination with immunotherapies are discussed. Simultaneous TGF-beta 1 and MDSCs inhibition suggest a potential improvement in immunotherapy or subverted tumor immune resistance.",
publisher = "Wiley",
journal = "Developmental Dinamics",
title = "Transforming growth factor-beta1 and myeloid-derived suppressor cells: A cancerous partnership",
pages = "124-105",
number = "1",
doi = "10.1002/dvdy.339"
}

Mojsilović, S., Mojsilović, S., Bjelica, S.,& Santibanez, J. F.. (2022). Transforming growth factor-beta1 and myeloid-derived suppressor cells: A cancerous partnership. in Developmental Dinamics
Wiley.(1), 105-124.
https://doi.org/10.1002/dvdy.339

Mojsilović S, Mojsilović S, Bjelica S, Santibanez JF. Transforming growth factor-beta1 and myeloid-derived suppressor cells: A cancerous partnership. in Developmental Dinamics. 2022;(1):105-124.
doi:10.1002/dvdy.339 .

Mojsilović, Slavko, Mojsilović, Sonja, Bjelica, Sunčica, Santibanez, Juan F., "Transforming growth factor-beta1 and myeloid-derived suppressor cells: A cancerous partnership" in Developmental Dinamics, no. 1 (2022):105-124,
https://doi.org/10.1002/dvdy.339 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Miletić, Maja
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1184
AB  - Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
PB  - Srpsko biološko društvo
T2  - Archives of Biological Sciences
T1  - Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
EP  - 24
IS  - 1
SP  - 15
VL  - 74
DO  - 10.2298/ABS210929048O
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Trivanović, Drenka and Petrović, Anđelija and Mojsilović, Slavko and Miletić, Maja and Bugarski, Diana and Jauković, Aleksandra",
year = "2022",
abstract = "Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.",
publisher = "Srpsko biološko društvo",
journal = "Archives of Biological Sciences",
title = "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway",
pages = "24-15",
number = "1",
volume = "74",
doi = "10.2298/ABS210929048O"
}

Okić Đorđević, I., Kukolj, T., Obradović, H., Trivanović, D., Petrović, A., Mojsilović, S., Miletić, M., Bugarski, D.,& Jauković, A.. (2022). Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences
Srpsko biološko društvo., 74(1), 15-24.
https://doi.org/10.2298/ABS210929048O

Okić Đorđević I, Kukolj T, Obradović H, Trivanović D, Petrović A, Mojsilović S, Miletić M, Bugarski D, Jauković A. Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences. 2022;74(1):15-24.
doi:10.2298/ABS210929048O .

Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Trivanović, Drenka, Petrović, Anđelija, Mojsilović, Slavko, Miletić, Maja, Bugarski, Diana, Jauković, Aleksandra, "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway" in Archives of Biological Sciences, 74, no. 1 (2022):15-24,
https://doi.org/10.2298/ABS210929048O . .

TY  - JOUR
AU  - Borojević, Ana
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Mojsilović, Slavko
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Živanović, Milena
AU  - Zečević, Željko
AU  - Simić, Marija
AU  - Gobeljić, Borko
AU  - Vujić, Dragana
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1207
AB  - The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T1  - Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
IS  - 2
SP  - 323
VL  - 12
DO  - 10.3390/biom12020323
ER  - 

@article{
author = "Borojević, Ana and Jauković, Aleksandra and Kukolj, Tamara and Mojsilović, Slavko and Obradović, Hristina and Trivanović, Drenka and Živanović, Milena and Zečević, Željko and Simić, Marija and Gobeljić, Borko and Vujić, Dragana and Bugarski, Diana",
year = "2022",
abstract = "The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules",
title = "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling",
number = "2",
pages = "323",
volume = "12",
doi = "10.3390/biom12020323"
}

Borojević, A., Jauković, A., Kukolj, T., Mojsilović, S., Obradović, H., Trivanović, D., Živanović, M., Zečević, Ž., Simić, M., Gobeljić, B., Vujić, D.,& Bugarski, D.. (2022). Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 323.
https://doi.org/10.3390/biom12020323

Borojević A, Jauković A, Kukolj T, Mojsilović S, Obradović H, Trivanović D, Živanović M, Zečević Ž, Simić M, Gobeljić B, Vujić D, Bugarski D. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules. 2022;12(2):323.
doi:10.3390/biom12020323 .

Borojević, Ana, Jauković, Aleksandra, Kukolj, Tamara, Mojsilović, Slavko, Obradović, Hristina, Trivanović, Drenka, Živanović, Milena, Zečević, Željko, Simić, Marija, Gobeljić, Borko, Vujić, Dragana, Bugarski, Diana, "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling" in Biomolecules, 12, no. 2 (2022):323,
https://doi.org/10.3390/biom12020323 . .

TY  - JOUR
AU  - Teixo, Ricardo
AU  - Pires, Ana Salomé
AU  - Pereira, Eurico
AU  - Serambeque, Beatriz
AU  - Marques, Inês Alexandra
AU  - Laranjo, Mafalda
AU  - Mojsilović, Slavko
AU  - Gramignoli, Roberto
AU  - Ponsaerts, Peter
AU  - Schoeberlein, Andreina
AU  - Botelho, Maria Filomena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1252
AB  - The increasing cancer incidence has certified oncological management as one of the most critical challenges for the coming decades. New anticancer strategies are still needed, despite the significant advances brought to the forefront in the last decades. The most recent, promising therapeutic approaches have benefitted from the application of human perinatal derivatives (PnD), biological mediators with proven benefits in several fields beyond oncology. To elucidate preclinical results and clinic outcomes achieved in the oncological field, we present a narrative review of the studies resorting to animal models to assess specific outcomes of PnD products. Recent preclinical evidence points to promising anticancer effects offered by PnD mediators isolated from the placenta, amniotic membrane, amniotic fluid, and umbilical cord. Described effects include tumorigenesis prevention, uncontrolled growth or regrowth inhibition, tumor homing ability, and adequate cell-based delivery capacity. Furthermore, PnD treatments have been described as supportive of chemotherapy and radiological therapies, particularly when resistance has been reported. However, opposite effects of PnD products have also been observed, offering support and trophic effect to malignant cells. Such paradoxical and dichotomous roles need to be intensively investigated. Current hypotheses identify as explanatory some critical factors, such as the type of the PnD biological products used or the manufacturing procedure to prepare the tissue/cellular treatment, the experimental design (including human-relevant animal models), and intrinsic pathophysiological characteristics. The effective and safe translation of PnD treatments to clinical practice relies on the collaborative efforts of all researchers working with human-relevant oncological preclinical models. However, it requires proper guidelines and consensus compiled by experts and health workers who accurately describe the methodology of tissue collection, PnD isolation, manufacturing, preservation, and delivery to the final user.
T2  - International Journal of Molecular Sciences
T1  - Application of Perinatal Derivatives on Oncological Preclinical Models: A Review of Animal Studies
IS  - 15
SP  - 8570
VL  - 23
DO  - 10.3390/ijms23158570
ER  - 

@article{
author = "Teixo, Ricardo and Pires, Ana Salomé and Pereira, Eurico and Serambeque, Beatriz and Marques, Inês Alexandra and Laranjo, Mafalda and Mojsilović, Slavko and Gramignoli, Roberto and Ponsaerts, Peter and Schoeberlein, Andreina and Botelho, Maria Filomena",
year = "2022",
abstract = "The increasing cancer incidence has certified oncological management as one of the most critical challenges for the coming decades. New anticancer strategies are still needed, despite the significant advances brought to the forefront in the last decades. The most recent, promising therapeutic approaches have benefitted from the application of human perinatal derivatives (PnD), biological mediators with proven benefits in several fields beyond oncology. To elucidate preclinical results and clinic outcomes achieved in the oncological field, we present a narrative review of the studies resorting to animal models to assess specific outcomes of PnD products. Recent preclinical evidence points to promising anticancer effects offered by PnD mediators isolated from the placenta, amniotic membrane, amniotic fluid, and umbilical cord. Described effects include tumorigenesis prevention, uncontrolled growth or regrowth inhibition, tumor homing ability, and adequate cell-based delivery capacity. Furthermore, PnD treatments have been described as supportive of chemotherapy and radiological therapies, particularly when resistance has been reported. However, opposite effects of PnD products have also been observed, offering support and trophic effect to malignant cells. Such paradoxical and dichotomous roles need to be intensively investigated. Current hypotheses identify as explanatory some critical factors, such as the type of the PnD biological products used or the manufacturing procedure to prepare the tissue/cellular treatment, the experimental design (including human-relevant animal models), and intrinsic pathophysiological characteristics. The effective and safe translation of PnD treatments to clinical practice relies on the collaborative efforts of all researchers working with human-relevant oncological preclinical models. However, it requires proper guidelines and consensus compiled by experts and health workers who accurately describe the methodology of tissue collection, PnD isolation, manufacturing, preservation, and delivery to the final user.",
journal = "International Journal of Molecular Sciences",
title = "Application of Perinatal Derivatives on Oncological Preclinical Models: A Review of Animal Studies",
number = "15",
pages = "8570",
volume = "23",
doi = "10.3390/ijms23158570"
}

Teixo, R., Pires, A. S., Pereira, E., Serambeque, B., Marques, I. A., Laranjo, M., Mojsilović, S., Gramignoli, R., Ponsaerts, P., Schoeberlein, A.,& Botelho, M. F.. (2022). Application of Perinatal Derivatives on Oncological Preclinical Models: A Review of Animal Studies. in International Journal of Molecular Sciences, 23(15), 8570.
https://doi.org/10.3390/ijms23158570

Teixo R, Pires AS, Pereira E, Serambeque B, Marques IA, Laranjo M, Mojsilović S, Gramignoli R, Ponsaerts P, Schoeberlein A, Botelho MF. Application of Perinatal Derivatives on Oncological Preclinical Models: A Review of Animal Studies. in International Journal of Molecular Sciences. 2022;23(15):8570.
doi:10.3390/ijms23158570 .

Teixo, Ricardo, Pires, Ana Salomé, Pereira, Eurico, Serambeque, Beatriz, Marques, Inês Alexandra, Laranjo, Mafalda, Mojsilović, Slavko, Gramignoli, Roberto, Ponsaerts, Peter, Schoeberlein, Andreina, Botelho, Maria Filomena, "Application of Perinatal Derivatives on Oncological Preclinical Models: A Review of Animal Studies" in International Journal of Molecular Sciences, 23, no. 15 (2022):8570,
https://doi.org/10.3390/ijms23158570 . .

TY  - JOUR
AU  - Gađanski, Ivana
AU  - Mojsilović, Slavko
AU  - Herrmann, Marietta
AU  - Krstić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1262
PB  - Frontiers Media S.A.
T2  - Frontiers in Cell and Developmental Biology
T1  - Editorial: Microenvironment-derived stem cell plasticity—volume II
SP  - 967461
VL  - 10
DO  - 10.3389/fcell.2022.967461
ER  - 

@article{
author = "Gađanski, Ivana and Mojsilović, Slavko and Herrmann, Marietta and Krstić, Jelena",
year = "2022",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cell and Developmental Biology",
title = "Editorial: Microenvironment-derived stem cell plasticity—volume II",
pages = "967461",
volume = "10",
doi = "10.3389/fcell.2022.967461"
}

Gađanski, I., Mojsilović, S., Herrmann, M.,& Krstić, J.. (2022). Editorial: Microenvironment-derived stem cell plasticity—volume II. in Frontiers in Cell and Developmental Biology
Frontiers Media S.A.., 10, 967461.
https://doi.org/10.3389/fcell.2022.967461

Gađanski I, Mojsilović S, Herrmann M, Krstić J. Editorial: Microenvironment-derived stem cell plasticity—volume II. in Frontiers in Cell and Developmental Biology. 2022;10:967461.
doi:10.3389/fcell.2022.967461 .

Gađanski, Ivana, Mojsilović, Slavko, Herrmann, Marietta, Krstić, Jelena, "Editorial: Microenvironment-derived stem cell plasticity—volume II" in Frontiers in Cell and Developmental Biology, 10 (2022):967461,
https://doi.org/10.3389/fcell.2022.967461 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Mojsilović, Slavko
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Saso, Luciano
AU  - Nicoletti, Marcello
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1086
AB  - Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration  gt = 500 mu g/mL. The antioxidant activity of hennosides (concentration  gt = 100 mu g/mL) was determined by FRAP and ABTS assays. At concentration of 500 mu g/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 +/- 0.08, 0.62 +/- 0.28 and 0.35 +/- 0.03 mM FeSO4 x 7H(2)O, and 0.15 +/- 0.01, 0.30 +/- 0.01 and 0.09 +/- 0.01 mM Trolox. Hennosides at 100 mu g/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.
PB  - MDPI
T2  - Plants-Basel
T1  - Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead
IS  - 2
SP  - 237
VL  - 10
DO  - 10.3390/plants10020237
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Drvenica, Ivana and Stančić, Ana and Mojsilović, Slavko and Kukolj, Tamara and Bugarski, Diana and Saso, Luciano and Nicoletti, Marcello",
year = "2021",
abstract = "Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration  gt = 500 mu g/mL. The antioxidant activity of hennosides (concentration  gt = 100 mu g/mL) was determined by FRAP and ABTS assays. At concentration of 500 mu g/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 +/- 0.08, 0.62 +/- 0.28 and 0.35 +/- 0.03 mM FeSO4 x 7H(2)O, and 0.15 +/- 0.01, 0.30 +/- 0.01 and 0.09 +/- 0.01 mM Trolox. Hennosides at 100 mu g/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.",
publisher = "MDPI",
journal = "Plants-Basel",
title = "Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead",
number = "2",
pages = "237",
volume = "10",
doi = "10.3390/plants10020237"
}

Maslovarić, I., Ilić, V., Drvenica, I., Stančić, A., Mojsilović, S., Kukolj, T., Bugarski, D., Saso, L.,& Nicoletti, M.. (2021). Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. in Plants-Basel
MDPI., 10(2), 237.
https://doi.org/10.3390/plants10020237

Maslovarić I, Ilić V, Drvenica I, Stančić A, Mojsilović S, Kukolj T, Bugarski D, Saso L, Nicoletti M. Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. in Plants-Basel. 2021;10(2):237.
doi:10.3390/plants10020237 .

Maslovarić, Irina, Ilić, Vesna, Drvenica, Ivana, Stančić, Ana, Mojsilović, Slavko, Kukolj, Tamara, Bugarski, Diana, Saso, Luciano, Nicoletti, Marcello, "Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead" in Plants-Basel, 10, no. 2 (2021):237,
https://doi.org/10.3390/plants10020237 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Miletić, Maja
AU  - Petrović, Vanja
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1149
AB  - Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
PB  - Wiley-Blackwell
T2  - Journal of Cellular Physiology
T1  - Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
EP  - 7341
IS  - 11
SP  - 7322
VL  - 236
DO  - 10.1002/jcp.30399
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Obradović, Hristina and Miletić, Maja and Petrović, Vanja and Mojsilović, Slavko and Bugarski, Diana",
year = "2021",
abstract = "Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.",
publisher = "Wiley-Blackwell",
journal = "Journal of Cellular Physiology",
title = "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF",
pages = "7341-7322",
number = "11",
volume = "236",
doi = "10.1002/jcp.30399"
}

Jauković, A., Kukolj, T., Trivanović, D., Okić Đorđević, I., Obradović, H., Miletić, M., Petrović, V., Mojsilović, S.,& Bugarski, D.. (2021). Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology
Wiley-Blackwell., 236(11), 7322-7341.
https://doi.org/10.1002/jcp.30399

Jauković A, Kukolj T, Trivanović D, Okić Đorđević I, Obradović H, Miletić M, Petrović V, Mojsilović S, Bugarski D. Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology. 2021;236(11):7322-7341.
doi:10.1002/jcp.30399 .

Jauković, Aleksandra, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Obradović, Hristina, Miletić, Maja, Petrović, Vanja, Mojsilović, Slavko, Bugarski, Diana, "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF" in Journal of Cellular Physiology, 236, no. 11 (2021):7322-7341,
https://doi.org/10.1002/jcp.30399 . .

TY  - JOUR
AU  - Mojsilović, Sonja
AU  - Mojsilović, Slavko
AU  - Villar, Victor H.
AU  - Santibanez, Juan F.
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1167
AB  - Besides transformed cells, the tumors are composed of various cell types that contribute to undesirable tumor progression. Tumor-associated macrophages (TAMs) are the most abundant innate immune cells in the tumor microenvironment (TME). Within the TME, TAMs exhibit high plasticity and undergo specific functional metabolic alterations according to the availability of tumor tissue oxygen and nutrients, thus further contributing to tumorigenesis and cancer progression. Here, we review the main functional TAM metabolic patterns influenced by TME, including glycolysis, amino acid, and fatty acid metabolism. Moreover, this review discusses antitumor immunotherapies that affect TAM functionality by inducing cell repolarizing and metabolic profiles towards an antitumoral phenotype. Also, new macrophage-based cell therapeutic technologies recently developed using chimeric antigen receptor bioengineering are exposed, which may overcome all solid tumor physical barriers impeding the current adoptive cell therapies and contribute to developing novel cancer immunotherapies.
PB  - Hindawi
T2  - Analytical Cellular Pathology
T1  - The Metabolic Features of Tumor-Associated Macrophages: Opportunities for Immunotherapy?
SP  - e5523055
VL  - 2021
DO  - 10.1155/2021/5523055
ER  - 

@article{
author = "Mojsilović, Sonja and Mojsilović, Slavko and Villar, Victor H. and Santibanez, Juan F.",
year = "2021",
abstract = "Besides transformed cells, the tumors are composed of various cell types that contribute to undesirable tumor progression. Tumor-associated macrophages (TAMs) are the most abundant innate immune cells in the tumor microenvironment (TME). Within the TME, TAMs exhibit high plasticity and undergo specific functional metabolic alterations according to the availability of tumor tissue oxygen and nutrients, thus further contributing to tumorigenesis and cancer progression. Here, we review the main functional TAM metabolic patterns influenced by TME, including glycolysis, amino acid, and fatty acid metabolism. Moreover, this review discusses antitumor immunotherapies that affect TAM functionality by inducing cell repolarizing and metabolic profiles towards an antitumoral phenotype. Also, new macrophage-based cell therapeutic technologies recently developed using chimeric antigen receptor bioengineering are exposed, which may overcome all solid tumor physical barriers impeding the current adoptive cell therapies and contribute to developing novel cancer immunotherapies.",
publisher = "Hindawi",
journal = "Analytical Cellular Pathology",
title = "The Metabolic Features of Tumor-Associated Macrophages: Opportunities for Immunotherapy?",
pages = "e5523055",
volume = "2021",
doi = "10.1155/2021/5523055"
}

Mojsilović, S., Mojsilović, S., Villar, V. H.,& Santibanez, J. F.. (2021). The Metabolic Features of Tumor-Associated Macrophages: Opportunities for Immunotherapy?. in Analytical Cellular Pathology
Hindawi., 2021, e5523055.
https://doi.org/10.1155/2021/5523055

Mojsilović S, Mojsilović S, Villar VH, Santibanez JF. The Metabolic Features of Tumor-Associated Macrophages: Opportunities for Immunotherapy?. in Analytical Cellular Pathology. 2021;2021:e5523055.
doi:10.1155/2021/5523055 .

Mojsilović, Sonja, Mojsilović, Slavko, Villar, Victor H., Santibanez, Juan F., "The Metabolic Features of Tumor-Associated Macrophages: Opportunities for Immunotherapy?" in Analytical Cellular Pathology, 2021 (2021):e5523055,
https://doi.org/10.1155/2021/5523055 . .

TY  - JOUR
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Petrović, Anđelija
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1175
AB  - As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Journal of Personalized Medicine
T1  - Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy
IS  - 11
SP  - 1133
VL  - 11
DO  - 10.3390/jpm11111133
ER  - 

@article{
author = "Mojsilović, Slavko and Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Petrović, Anđelija and Bugarski, Diana",
year = "2021",
abstract = "As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Journal of Personalized Medicine",
title = "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy",
number = "11",
pages = "1133",
volume = "11",
doi = "10.3390/jpm11111133"
}

Mojsilović, S., Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Petrović, A.,& Bugarski, D.. (2021). Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine
Multidisciplinary Digital Publishing Institute (MDPI)., 11(11), 1133.
https://doi.org/10.3390/jpm11111133

Mojsilović S, Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Petrović A, Bugarski D. Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine. 2021;11(11):1133.
doi:10.3390/jpm11111133 .

Mojsilović, Slavko, Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Petrović, Anđelija, Bugarski, Diana, "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy" in Journal of Personalized Medicine, 11, no. 11 (2021):1133,
https://doi.org/10.3390/jpm11111133 . .

TY  - JOUR
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Stančić, Ana
AU  - Marković, Dragana
AU  - Kovačić, Marijana
AU  - Maslovarić, Irina
AU  - Rapajić, Ivana
AU  - Vučetić, Dušan
AU  - Ilić, Vesna
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1176
AB  - Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.
PB  - Springer Nature
T2  - European Biophysics Journal
T1  - The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method
EP  - 846
IS  - 6
SP  - 829
VL  - 50
DO  - 10.1007/s00249-021-01527-3
ER  - 

@article{
author = "Drvenica, Ivana and Mojsilović, Slavko and Stančić, Ana and Marković, Dragana and Kovačić, Marijana and Maslovarić, Irina and Rapajić, Ivana and Vučetić, Dušan and Ilić, Vesna",
year = "2021",
abstract = "Flow cytometry (FC) analysis of erythrocyte shape and related biomechanical properties, such as osmotic fragility, have not moved from a research tool to regular clinical testing. The main reason is existing evidence that various pre-analytical factors influence the mathematical interpretation of the data obtained. With an aim to contribute to the standardization and broaden the use of FC for human erythrocyte shape assessment, freshly prepared peripheral blood erythrocytes isolated from healthy donors were incubated in iso and hypo-osmotic solutions (pure saline, saline with potassium and calcium, and phosphate buffered saline) and examined by FC using values of forward scatter (FSC) and side scatter (SSC). Kurtosis, skewness, Pearson's second skewness coefficient of dissymmetry (PCD), and spherical index, calculated from FSC distributions, were used for the erythrocyte shape evaluation. In all isotonic media FSC distribution and FSC-based morphology parameters showed huge inter-individual and inter-medium variation. With decreasing osmolality, in all media and samples, the size of the erythrocytes increased, and swelling index and kurtosis decreased. However, changes in skewness and PCD were influenced by the medium used and the sample tested. Compared to FSC, SSC signal in isotonic and its change in hypotonic media showed lower inter-individual variation and was not influenced by the type of medium. We propose a spherical index and kurtosis as FSC-based indicators of erythrocyte shape. As more resistant to the influence of the preanalytical treatment, SSC data appeared to be unfairly neglected for the assessment of erythrocyte shape, in comparison to the usually employed FSC data.",
publisher = "Springer Nature",
journal = "European Biophysics Journal",
title = "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method",
pages = "846-829",
number = "6",
volume = "50",
doi = "10.1007/s00249-021-01527-3"
}

Drvenica, I., Mojsilović, S., Stančić, A., Marković, D., Kovačić, M., Maslovarić, I., Rapajić, I., Vučetić, D.,& Ilić, V.. (2021). The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal
Springer Nature., 50(6), 829-846.
https://doi.org/10.1007/s00249-021-01527-3

Drvenica I, Mojsilović S, Stančić A, Marković D, Kovačić M, Maslovarić I, Rapajić I, Vučetić D, Ilić V. The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method. in European Biophysics Journal. 2021;50(6):829-846.
doi:10.1007/s00249-021-01527-3 .

Drvenica, Ivana, Mojsilović, Slavko, Stančić, Ana, Marković, Dragana, Kovačić, Marijana, Maslovarić, Irina, Rapajić, Ivana, Vučetić, Dušan, Ilić, Vesna, "The effects of incubation media on the assessment of the shape of human erythrocytes by flow cytometry: a contribution to mathematical data interpretation to enable wider application of the method" in European Biophysics Journal, 50, no. 6 (2021):829-846,
https://doi.org/10.1007/s00249-021-01527-3 . .

TY  - JOUR
AU  - Pichlsberger, Melanie
AU  - Jerman, Urška Dragin
AU  - Obradović, Hristina
AU  - Tratnjek, Larisa
AU  - Macedo, Ana Sofia
AU  - Mendes, Francisca
AU  - Fonte, Pedro
AU  - Hoegler, Anja
AU  - Sundl, Monika
AU  - Fuchs, Julia
AU  - Schoeberlein, Andreina
AU  - Kreft, Mateja Erdani
AU  - Mojsilović, Slavko
AU  - Lang-Olip, Ingrid
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1177
AB  - Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific interest, as they can be obtained non-invasively, have anti-inflammatory, anti-cancer, and anti-fibrotic characteristics, and are immunologically tolerated in vivo. Many studies that apply PnD in pre-clinical cutaneous wound healing models show large variations in the choice of the animal species (e.g., large animals, rodents), the choice of diabetic or non-diabetic animals, the type of injury (full-thickness wounds, burns, radiation-induced wounds, skin flaps), the source and type of PnD (placenta, umbilical cord, fetal membranes, cells, secretomes, tissue extracts), the method of administration (topical application, intradermal/subcutaneous injection, intravenous or intraperitoneal injection, subcutaneous implantation), and the type of delivery systems (e.g., hydrogels, synthetic or natural biomaterials as carriers for transplanted cells, extracts or secretomes). This review provides a comprehensive and integrative overview of the application of PnD in wound healing to assess its efficacy in preclinical animal models. We highlight the advantages and limitations of the most commonly used animal models and evaluate the impact of the type of PnD, the route of administration, and the dose of cells/secretome application in correlation with the wound healing outcome. This review is a collaborative effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the preclinical application of PnD in wound healing.
PB  - Frontiers Media S.A.
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing
SP  - 742858
VL  - 9
DO  - 10.3389/fbioe.2021.742858
ER  - 

@article{
author = "Pichlsberger, Melanie and Jerman, Urška Dragin and Obradović, Hristina and Tratnjek, Larisa and Macedo, Ana Sofia and Mendes, Francisca and Fonte, Pedro and Hoegler, Anja and Sundl, Monika and Fuchs, Julia and Schoeberlein, Andreina and Kreft, Mateja Erdani and Mojsilović, Slavko and Lang-Olip, Ingrid",
year = "2021",
abstract = "Knowledge of the beneficial effects of perinatal derivatives (PnD) in wound healing goes back to the early 1900s when the human fetal amniotic membrane served as a biological dressing to treat burns and skin ulcerations. Since the twenty-first century, isolated cells from perinatal tissues and their secretomes have gained increasing scientific interest, as they can be obtained non-invasively, have anti-inflammatory, anti-cancer, and anti-fibrotic characteristics, and are immunologically tolerated in vivo. Many studies that apply PnD in pre-clinical cutaneous wound healing models show large variations in the choice of the animal species (e.g., large animals, rodents), the choice of diabetic or non-diabetic animals, the type of injury (full-thickness wounds, burns, radiation-induced wounds, skin flaps), the source and type of PnD (placenta, umbilical cord, fetal membranes, cells, secretomes, tissue extracts), the method of administration (topical application, intradermal/subcutaneous injection, intravenous or intraperitoneal injection, subcutaneous implantation), and the type of delivery systems (e.g., hydrogels, synthetic or natural biomaterials as carriers for transplanted cells, extracts or secretomes). This review provides a comprehensive and integrative overview of the application of PnD in wound healing to assess its efficacy in preclinical animal models. We highlight the advantages and limitations of the most commonly used animal models and evaluate the impact of the type of PnD, the route of administration, and the dose of cells/secretome application in correlation with the wound healing outcome. This review is a collaborative effort from the COST SPRINT Action (CA17116), which broadly aims at approaching consensus for different aspects of PnD research, such as providing inputs for future standards for the preclinical application of PnD in wound healing.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing",
pages = "742858",
volume = "9",
doi = "10.3389/fbioe.2021.742858"
}

Pichlsberger, M., Jerman, U. D., Obradović, H., Tratnjek, L., Macedo, A. S., Mendes, F., Fonte, P., Hoegler, A., Sundl, M., Fuchs, J., Schoeberlein, A., Kreft, M. E., Mojsilović, S.,& Lang-Olip, I.. (2021). Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing. in Frontiers in Bioengineering and Biotechnology
Frontiers Media S.A.., 9, 742858.
https://doi.org/10.3389/fbioe.2021.742858

Pichlsberger M, Jerman UD, Obradović H, Tratnjek L, Macedo AS, Mendes F, Fonte P, Hoegler A, Sundl M, Fuchs J, Schoeberlein A, Kreft ME, Mojsilović S, Lang-Olip I. Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing. in Frontiers in Bioengineering and Biotechnology. 2021;9:742858.
doi:10.3389/fbioe.2021.742858 .

Pichlsberger, Melanie, Jerman, Urška Dragin, Obradović, Hristina, Tratnjek, Larisa, Macedo, Ana Sofia, Mendes, Francisca, Fonte, Pedro, Hoegler, Anja, Sundl, Monika, Fuchs, Julia, Schoeberlein, Andreina, Kreft, Mateja Erdani, Mojsilović, Slavko, Lang-Olip, Ingrid, "Systematic Review of the Application of Perinatal Derivatives in Animal Models on Cutaneous Wound Healing" in Frontiers in Bioengineering and Biotechnology, 9 (2021):742858,
https://doi.org/10.3389/fbioe.2021.742858 . .

TY  - JOUR
AU  - Janev, Aleksandar
AU  - Ramuta, Taja Železnik
AU  - Tratnjek, Larisa
AU  - Sardoč, Žiga
AU  - Obradović, Hristina
AU  - Mojsilović, Slavko
AU  - Taskovska, Milena
AU  - Smrkolj, Tomaž
AU  - Kreft, Mateja Erdani
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1179
AB  - Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland non-tumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.
PB  - Frontiers Media S.A.
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models
SP  - 690358
VL  - 9
DO  - 10.3389/fbioe.2021.690358
ER  - 

@article{
author = "Janev, Aleksandar and Ramuta, Taja Železnik and Tratnjek, Larisa and Sardoč, Žiga and Obradović, Hristina and Mojsilović, Slavko and Taskovska, Milena and Smrkolj, Tomaž and Kreft, Mateja Erdani",
year = "2021",
abstract = "Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland non-tumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models",
pages = "690358",
volume = "9",
doi = "10.3389/fbioe.2021.690358"
}

Janev, A., Ramuta, T. Ž., Tratnjek, L., Sardoč, Ž., Obradović, H., Mojsilović, S., Taskovska, M., Smrkolj, T.,& Kreft, M. E.. (2021). Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models. in Frontiers in Bioengineering and Biotechnology
Frontiers Media S.A.., 9, 690358.
https://doi.org/10.3389/fbioe.2021.690358

Janev A, Ramuta TŽ, Tratnjek L, Sardoč Ž, Obradović H, Mojsilović S, Taskovska M, Smrkolj T, Kreft ME. Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models. in Frontiers in Bioengineering and Biotechnology. 2021;9:690358.
doi:10.3389/fbioe.2021.690358 .

Janev, Aleksandar, Ramuta, Taja Železnik, Tratnjek, Larisa, Sardoč, Žiga, Obradović, Hristina, Mojsilović, Slavko, Taskovska, Milena, Smrkolj, Tomaž, Kreft, Mateja Erdani, "Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models" in Frontiers in Bioengineering and Biotechnology, 9 (2021):690358,
https://doi.org/10.3389/fbioe.2021.690358 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Kukolj, Tamara
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1183
AB  - Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.
PB  - Baishideng Publishing Group Inc
T2  - World Journal of Stem Cells
T1  - Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy
EP  - 1880
IS  - 12
SP  - 1863
VL  - 13
DO  - 10.4252/wjsc.v13.i12.1863
ER  - 

@article{
author = "Okić Đorđević, Ivana and Obradović, Hristina and Kukolj, Tamara and Petrović, Anđelija and Mojsilović, Slavko and Bugarski, Diana and Jauković, Aleksandra",
year = "2021",
abstract = "Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.",
publisher = "Baishideng Publishing Group Inc",
journal = "World Journal of Stem Cells",
title = "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy",
pages = "1880-1863",
number = "12",
volume = "13",
doi = "10.4252/wjsc.v13.i12.1863"
}

Okić Đorđević, I., Obradović, H., Kukolj, T., Petrović, A., Mojsilović, S., Bugarski, D.,& Jauković, A.. (2021). Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells
Baishideng Publishing Group Inc., 13(12), 1863-1880.
https://doi.org/10.4252/wjsc.v13.i12.1863

Okić Đorđević I, Obradović H, Kukolj T, Petrović A, Mojsilović S, Bugarski D, Jauković A. Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells. 2021;13(12):1863-1880.
doi:10.4252/wjsc.v13.i12.1863 .

Okić Đorđević, Ivana, Obradović, Hristina, Kukolj, Tamara, Petrović, Anđelija, Mojsilović, Slavko, Bugarski, Diana, Jauković, Aleksandra, "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy" in World Journal of Stem Cells, 13, no. 12 (2021):1863-1880,
https://doi.org/10.4252/wjsc.v13.i12.1863 . .

TY  - JOUR
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Mojsilović, Sonja
AU  - Santibanez, Juan F.
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1220
AB  - Bone regeneration is a tightly regulated process that ensures proper repair and functionality after injury. The delicate balance between bone formation and resorption is governed by cytokines and signaling molecules released during the inflammatory response. Interleukin (IL)-17A, produced in the early phase of inflammation, influences the fate of osteoprogenitors. Due to their inherent capacity to differentiate into osteoblasts, mesenchymal stem/stromal cells (MSCs) contribute to bone healing and regeneration. This review presents an overview of IL-17A signaling and the leading cellular and molecular mechanisms by which it regulates the osteogenic differentiation of MSCs. The main findings demonstrating IL-17A’s influence on osteoblastogenesis are described. To this end, divergent information exists about the capacity of IL-17A to regulate MSCs’ osteogenic fate, depending on the tissue context and target cell type, along with contradictory findings in the same cell types. Therefore, we summarize the data showing both the pro-osteogenic and anti-osteogenic roles of IL-17, which may help in the understanding of IL-17A function in bone repair and regeneration.
PB  - Baishideng Publishing Group
T2  - World Journal of Stem Cells
T1  - Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation
EP  - 1713
IS  - 11
SP  - 1696
VL  - 13
DO  - 10.4252/wjsc.v13.i11.1696
ER  - 

@article{
author = "Krstić, Jelena and Mojsilović, Slavko and Mojsilović, Sonja and Santibanez, Juan F.",
year = "2021",
abstract = "Bone regeneration is a tightly regulated process that ensures proper repair and functionality after injury. The delicate balance between bone formation and resorption is governed by cytokines and signaling molecules released during the inflammatory response. Interleukin (IL)-17A, produced in the early phase of inflammation, influences the fate of osteoprogenitors. Due to their inherent capacity to differentiate into osteoblasts, mesenchymal stem/stromal cells (MSCs) contribute to bone healing and regeneration. This review presents an overview of IL-17A signaling and the leading cellular and molecular mechanisms by which it regulates the osteogenic differentiation of MSCs. The main findings demonstrating IL-17A’s influence on osteoblastogenesis are described. To this end, divergent information exists about the capacity of IL-17A to regulate MSCs’ osteogenic fate, depending on the tissue context and target cell type, along with contradictory findings in the same cell types. Therefore, we summarize the data showing both the pro-osteogenic and anti-osteogenic roles of IL-17, which may help in the understanding of IL-17A function in bone repair and regeneration.",
publisher = "Baishideng Publishing Group",
journal = "World Journal of Stem Cells",
title = "Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation",
pages = "1713-1696",
number = "11",
volume = "13",
doi = "10.4252/wjsc.v13.i11.1696"
}

Krstić, J., Mojsilović, S., Mojsilović, S.,& Santibanez, J. F.. (2021). Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation. in World Journal of Stem Cells
Baishideng Publishing Group., 13(11), 1696-1713.
https://doi.org/10.4252/wjsc.v13.i11.1696

Krstić J, Mojsilović S, Mojsilović S, Santibanez JF. Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation. in World Journal of Stem Cells. 2021;13(11):1696-1713.
doi:10.4252/wjsc.v13.i11.1696 .

Krstić, Jelena, Mojsilović, Slavko, Mojsilović, Sonja, Santibanez, Juan F., "Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation" in World Journal of Stem Cells, 13, no. 11 (2021):1696-1713,
https://doi.org/10.4252/wjsc.v13.i11.1696 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/993
AB  - Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Stem Cells
T1  - Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators
EP  - 937
IS  - 9
SP  - 922
VL  - 12
DO  - 10.4252/wjsc.v12.i9.922
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Bugarski, Diana",
year = "2020",
abstract = "Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Stem Cells",
title = "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators",
pages = "937-922",
number = "9",
volume = "12",
doi = "10.4252/wjsc.v12.i9.922"
}

Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S.,& Bugarski, D.. (2020). Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells
Baishideng Publishing Group Inc, Pleasanton., 12(9), 922-937.
https://doi.org/10.4252/wjsc.v12.i9.922

Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Bugarski D. Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells. 2020;12(9):922-937.
doi:10.4252/wjsc.v12.i9.922 .

Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Bugarski, Diana, "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators" in World Journal of Stem Cells, 12, no. 9 (2020):922-937,
https://doi.org/10.4252/wjsc.v12.i9.922 . .