TY  - JOUR
AU  - Miletić, Maja
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Brković, Božidar
AU  - Andrić, Miroslav
AU  - Prokić, Bogomir Bolka
AU  - Danilović, Vesna
AU  - Milutinović-Smiljanić, Sanja
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1464
AB  - In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.
T2  - Applied Sciences
T2  - Applied Sciences
T1  - Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment
IS  - 1
SP  - 16
VL  - 14
DO  - 10.3390/app14010016
ER  - 

@article{
author = "Miletić, Maja and Puač, Nevena and Škoro, Nikola and Brković, Božidar and Andrić, Miroslav and Prokić, Bogomir Bolka and Danilović, Vesna and Milutinović-Smiljanić, Sanja and Mitrović-Ajtić, Olivera and Mojsilović, Slavko",
year = "2024",
abstract = "In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.",
journal = "Applied Sciences, Applied Sciences",
title = "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment",
number = "1",
pages = "16",
volume = "14",
doi = "10.3390/app14010016"
}

Miletić, M., Puač, N., Škoro, N., Brković, B., Andrić, M., Prokić, B. B., Danilović, V., Milutinović-Smiljanić, S., Mitrović-Ajtić, O.,& Mojsilović, S.. (2024). Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences, 14(1), 16.
https://doi.org/10.3390/app14010016

Miletić M, Puač N, Škoro N, Brković B, Andrić M, Prokić BB, Danilović V, Milutinović-Smiljanić S, Mitrović-Ajtić O, Mojsilović S. Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences. 2024;14(1):16.
doi:10.3390/app14010016 .

Miletić, Maja, Puač, Nevena, Škoro, Nikola, Brković, Božidar, Andrić, Miroslav, Prokić, Bogomir Bolka, Danilović, Vesna, Milutinović-Smiljanić, Sanja, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment" in Applied Sciences, 14, no. 1 (2024):16,
https://doi.org/10.3390/app14010016 . .