TY  - JOUR
AU  - Miletić, Maja
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Brković, Božidar
AU  - Andrić, Miroslav
AU  - Prokić, Bogomir Bolka
AU  - Danilović, Vesna
AU  - Milutinović-Smiljanić, Sanja
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1464
AB  - In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.
T2  - Applied Sciences
T2  - Applied Sciences
T1  - Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment
IS  - 1
SP  - 16
VL  - 14
DO  - 10.3390/app14010016
ER  - 

@article{
author = "Miletić, Maja and Puač, Nevena and Škoro, Nikola and Brković, Božidar and Andrić, Miroslav and Prokić, Bogomir Bolka and Danilović, Vesna and Milutinović-Smiljanić, Sanja and Mitrović-Ajtić, Olivera and Mojsilović, Slavko",
year = "2024",
abstract = "In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.",
journal = "Applied Sciences, Applied Sciences",
title = "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment",
number = "1",
pages = "16",
volume = "14",
doi = "10.3390/app14010016"
}

Miletić, M., Puač, N., Škoro, N., Brković, B., Andrić, M., Prokić, B. B., Danilović, V., Milutinović-Smiljanić, S., Mitrović-Ajtić, O.,& Mojsilović, S.. (2024). Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences, 14(1), 16.
https://doi.org/10.3390/app14010016

Miletić M, Puač N, Škoro N, Brković B, Andrić M, Prokić BB, Danilović V, Milutinović-Smiljanić S, Mitrović-Ajtić O, Mojsilović S. Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences. 2024;14(1):16.
doi:10.3390/app14010016 .

Miletić, Maja, Puač, Nevena, Škoro, Nikola, Brković, Božidar, Andrić, Miroslav, Prokić, Bogomir Bolka, Danilović, Vesna, Milutinović-Smiljanić, Sanja, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment" in Applied Sciences, 14, no. 1 (2024):16,
https://doi.org/10.3390/app14010016 . .

TY  - CONF
AU  - Miletić, Maja
AU  - Pavlović, Ognjan
AU  - Škoro, Nikola
AU  - Lazarević, Miloš
AU  - Jakovljević, Aleksandar
AU  - Mojsilović, Slavko
AU  - Puač, Nevena
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1431
AB  - Oral squamous cell carcinoma (OSCC) is one of the most malignant neoplasms of the oral cavity, with a high mortality rate. Since the long-term survival rate of patients diagnosed with OSCC has remained unchanged over the past several decades, it is of utmost importance to discover new treatment modalities or enhance existing ones [1]. Since we previously demonstrated the antitumor efficacy of cold atmospheric pressure plasma (CAP) on OSCC cell lines in a two-dimensional (2D) monolayer cell model, we moved further and tried to explain the mechanism of these cytotoxic events as well as analyze the effect of plasma-activated medium (PAM) in combination with chemotherapy as a gold standard in carcinoma treatment. The modified plasma needle operating at 13.56MHz with He as working gas was used for PAM generation with the distance of 3 mm between liquid surface and the tube and applying different exposure intervals [2]. OSCC cell line (SCC25) was cultivated in 2D or 3D culture systems when regular culture medium was changed for PAM for 24 h before performing assays. PAM treatment showed cytotoxic effects on 2D-cultured OSCC by inducing apoptotic cell death through the activation of the intrinsic caspase pathway. To analyze the combined effect of cisplatin and PAM we used a 3D cell culture approach with OSCC spheroids, as this method reflects more closely the in vivo cellular response to chemotherapeutics [3]. When PAM was combined with the increasing concentrations of cisplatin, the results showed an almost linear dose dependent decrease in OSCC spheroid viability. These arepromising and encouraging results for a potential application of CAP in the treatment of oral carcinoma. By combining the effects of chemotherapeutics with PAM, we developed a new prospect for a possible cancer treatment in which the same or even better antitumor effects could be achieved with lower doses of cytotoxic drugs. Consequently, it means that with lower doses of chemotherapeutics, we could minimize potential side effects associated with the high-dose usage of cytostatics and improve the quality of life for patients with these malignancies.
C3  - 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy
T1  - Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1431
ER  - 

@conference{
author = "Miletić, Maja and Pavlović, Ognjan and Škoro, Nikola and Lazarević, Miloš and Jakovljević, Aleksandar and Mojsilović, Slavko and Puač, Nevena",
year = "2023",
abstract = "Oral squamous cell carcinoma (OSCC) is one of the most malignant neoplasms of the oral cavity, with a high mortality rate. Since the long-term survival rate of patients diagnosed with OSCC has remained unchanged over the past several decades, it is of utmost importance to discover new treatment modalities or enhance existing ones [1]. Since we previously demonstrated the antitumor efficacy of cold atmospheric pressure plasma (CAP) on OSCC cell lines in a two-dimensional (2D) monolayer cell model, we moved further and tried to explain the mechanism of these cytotoxic events as well as analyze the effect of plasma-activated medium (PAM) in combination with chemotherapy as a gold standard in carcinoma treatment. The modified plasma needle operating at 13.56MHz with He as working gas was used for PAM generation with the distance of 3 mm between liquid surface and the tube and applying different exposure intervals [2]. OSCC cell line (SCC25) was cultivated in 2D or 3D culture systems when regular culture medium was changed for PAM for 24 h before performing assays. PAM treatment showed cytotoxic effects on 2D-cultured OSCC by inducing apoptotic cell death through the activation of the intrinsic caspase pathway. To analyze the combined effect of cisplatin and PAM we used a 3D cell culture approach with OSCC spheroids, as this method reflects more closely the in vivo cellular response to chemotherapeutics [3]. When PAM was combined with the increasing concentrations of cisplatin, the results showed an almost linear dose dependent decrease in OSCC spheroid viability. These arepromising and encouraging results for a potential application of CAP in the treatment of oral carcinoma. By combining the effects of chemotherapeutics with PAM, we developed a new prospect for a possible cancer treatment in which the same or even better antitumor effects could be achieved with lower doses of cytotoxic drugs. Consequently, it means that with lower doses of chemotherapeutics, we could minimize potential side effects associated with the high-dose usage of cytostatics and improve the quality of life for patients with these malignancies.",
journal = "2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy",
title = "Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1431"
}

Miletić, M., Pavlović, O., Škoro, N., Lazarević, M., Jakovljević, A., Mojsilović, S.,& Puač, N.. (2023). Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models. in 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy, 82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1431

Miletić M, Pavlović O, Škoro N, Lazarević M, Jakovljević A, Mojsilović S, Puač N. Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models. in 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy. 2023;:82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1431 .

Miletić, Maja, Pavlović, Ognjan, Škoro, Nikola, Lazarević, Miloš, Jakovljević, Aleksandar, Mojsilović, Slavko, Puač, Nevena, "Modulating chemosensitivity of oral carcinoma to Cisplatin by combination with plasma activated medium on 3D cell models" in 2nd Annual Meeting of COST Action CA20114 PlasTHER “Therapeutical applications of cold plasmas”,  4-7 September 2023, Bologna, Italy (2023):82-82,
https://hdl.handle.net/21.15107/rcub_rimi_1431 .