The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis
Аутори
Okić Đorđević, IvanaKukolj, Tamara
Živanović, Milena
Momčilović, Sanja
Obradović, Hristina
Petrović, Anđelija
Mojsilović, Slavko
Trivanović, Drenka
Jauković, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibit...ory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.
Кључне речи:
doxycycline / periodontal ligament MSCs / Interleukin 17 / regenerative propertiesИзвор:
Biomolecules, 2023, 13, 10, 1437-Издавач:
- Multidisciplinary Digital Publishing Institute (MDPI)
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200015 (Универзитет у Београду, Институт за медицинска истраживања) (RS-MESTD-inst-2020-200015)
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Okić Đorđević, Ivana AU - Kukolj, Tamara AU - Živanović, Milena AU - Momčilović, Sanja AU - Obradović, Hristina AU - Petrović, Anđelija AU - Mojsilović, Slavko AU - Trivanović, Drenka AU - Jauković, Aleksandra PY - 2023 UR - http://rimi.imi.bg.ac.rs/handle/123456789/1346 AB - Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed. PB - Multidisciplinary Digital Publishing Institute (MDPI) T2 - Biomolecules T2 - Biomolecules T1 - The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis IS - 10 SP - 1437 VL - 13 DO - 10.3390/biom13101437 ER -
@article{ author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra", year = "2023", abstract = "Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.", publisher = "Multidisciplinary Digital Publishing Institute (MDPI)", journal = "Biomolecules, Biomolecules", title = "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis", number = "10", pages = "1437", volume = "13", doi = "10.3390/biom13101437" }
Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules Multidisciplinary Digital Publishing Institute (MDPI)., 13(10), 1437. https://doi.org/10.3390/biom13101437
Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules. 2023;13(10):1437. doi:10.3390/biom13101437 .
Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis" in Biomolecules, 13, no. 10 (2023):1437, https://doi.org/10.3390/biom13101437 . .