TY  - JOUR
AU  - Janev, Aleksandar
AU  - Ramuta, Taja Železnik
AU  - Tratnjek, Larisa
AU  - Sardoč, Žiga
AU  - Obradović, Hristina
AU  - Mojsilović, Slavko
AU  - Taskovska, Milena
AU  - Smrkolj, Tomaž
AU  - Kreft, Mateja Erdani
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1179
AB  - Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland non-tumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.
PB  - Frontiers Media S.A.
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models
SP  - 690358
VL  - 9
DO  - 10.3389/fbioe.2021.690358
ER  - 

@article{
author = "Janev, Aleksandar and Ramuta, Taja Železnik and Tratnjek, Larisa and Sardoč, Žiga and Obradović, Hristina and Mojsilović, Slavko and Taskovska, Milena and Smrkolj, Tomaž and Kreft, Mateja Erdani",
year = "2021",
abstract = "Despite being among the ten most common cancers with high recurrence rates worldwide, there have been no major breakthroughs in the standard treatment options for bladder cancer in recent years. The use of a human amniotic membrane (hAM) to treat cancer is one of the promising ideas that have emerged in recent years. This study aimed to investigate the anticancer activity of hAM homogenate on 2D and 3D cancer models. We evaluated the effects of hAM homogenates on the human muscle invasive bladder cancer urothelial (T24) cells, papillary cancer urothelial (RT4) cells and normal porcine urothelial (NPU) cells as well as on human mammary gland non-tumorigenic (MCF10a) cells and low-metastatic breast cancer (MCF7) cells. After 24 h, we observed a gradual detachment of cancerous cells from the culture surface, while the hAM homogenate did not affect the normal cells. The most pronounced effect hAM homogenate had on bladder cancer cells; however, the potency of their detachment was dependent on the treatment protocol and the preparation of hAM homogenate. We demonstrated that hAM homogenate significantly decreased the adhesion, growth, and proliferation of human bladder invasive and papillary cancer urothelial cells and did not affect normal urothelial cells even in 7-day treatment. By using light and electron microscopy we showed that hAM homogenate disrupted the architecture of 2D and 3D bladder cancer models. The information provided by our study highlights the detrimental effect of hAM homogenate on bladder cancer cells and strengthens the idea of the potential clinical application of hAM for bladder cancer treatment.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models",
pages = "690358",
volume = "9",
doi = "10.3389/fbioe.2021.690358"
}

Janev, A., Ramuta, T. Ž., Tratnjek, L., Sardoč, Ž., Obradović, H., Mojsilović, S., Taskovska, M., Smrkolj, T.,& Kreft, M. E.. (2021). Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models. in Frontiers in Bioengineering and Biotechnology
Frontiers Media S.A.., 9, 690358.
https://doi.org/10.3389/fbioe.2021.690358

Janev A, Ramuta TŽ, Tratnjek L, Sardoč Ž, Obradović H, Mojsilović S, Taskovska M, Smrkolj T, Kreft ME. Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models. in Frontiers in Bioengineering and Biotechnology. 2021;9:690358.
doi:10.3389/fbioe.2021.690358 .

Janev, Aleksandar, Ramuta, Taja Železnik, Tratnjek, Larisa, Sardoč, Žiga, Obradović, Hristina, Mojsilović, Slavko, Taskovska, Milena, Smrkolj, Tomaž, Kreft, Mateja Erdani, "Detrimental Effect of Various Preparations of the Human Amniotic Membrane Homogenate on the 2D and 3D Bladder Cancer In vitro Models" in Frontiers in Bioengineering and Biotechnology, 9 (2021):690358,
https://doi.org/10.3389/fbioe.2021.690358 . .