TY  - JOUR
AU  - Miletić, Maja
AU  - Puač, Nevena
AU  - Škoro, Nikola
AU  - Brković, Božidar
AU  - Andrić, Miroslav
AU  - Prokić, Bogomir Bolka
AU  - Danilović, Vesna
AU  - Milutinović-Smiljanić, Sanja
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1464
AB  - In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.
T2  - Applied Sciences
T2  - Applied Sciences
T1  - Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment
IS  - 1
SP  - 16
VL  - 14
DO  - 10.3390/app14010016
ER  - 

@article{
author = "Miletić, Maja and Puač, Nevena and Škoro, Nikola and Brković, Božidar and Andrić, Miroslav and Prokić, Bogomir Bolka and Danilović, Vesna and Milutinović-Smiljanić, Sanja and Mitrović-Ajtić, Olivera and Mojsilović, Slavko",
year = "2024",
abstract = "In regenerative bone tissue medicine, combining artificial bone substitutes with progenitor cells is a prospective approach. Surface modification via cold atmospheric plasma (CAP) enhances biomaterial–cell interactions, which are crucial for successful bone regeneration. Using a rabbit calvarial critical-size defect model, we assessed the use of CAP-pretreated beta-tricalcium phosphate (β-TCP), alone or with periodontal ligament stem cells (PDLSCs), for bone regeneration. Histological and histomorphometric analyses at two and four weeks revealed significantly improved bone regeneration and reduced inflammation in the CAP-treated β-TCP with PDLSCs compared to β-TCP alone. Immunohistochemical analysis also showed an increase in the bone healing markers, including bone morphogenic proteins 2 and 4, runt-related transcription factor 2, collagen-1, and osteonectin, after two and four weeks in the CAP-treated β-TCP implants with PDLSC. This in vivo study demonstrates for the first time the superior bone regenerative capacity of CAP-pretreated β-TCP seeded with PDLSCs, highlighting the therapeutic potential of this combined approach in osteoregeneration.",
journal = "Applied Sciences, Applied Sciences",
title = "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment",
number = "1",
pages = "16",
volume = "14",
doi = "10.3390/app14010016"
}

Miletić, M., Puač, N., Škoro, N., Brković, B., Andrić, M., Prokić, B. B., Danilović, V., Milutinović-Smiljanić, S., Mitrović-Ajtić, O.,& Mojsilović, S.. (2024). Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences, 14(1), 16.
https://doi.org/10.3390/app14010016

Miletić M, Puač N, Škoro N, Brković B, Andrić M, Prokić BB, Danilović V, Milutinović-Smiljanić S, Mitrović-Ajtić O, Mojsilović S. Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment. in Applied Sciences. 2024;14(1):16.
doi:10.3390/app14010016 .

Miletić, Maja, Puač, Nevena, Škoro, Nikola, Brković, Božidar, Andrić, Miroslav, Prokić, Bogomir Bolka, Danilović, Vesna, Milutinović-Smiljanić, Sanja, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, "Bone Regeneration Potential of Periodontal Ligament Stem Cells in Combination with Cold Atmospheric Plasma-Pretreated Beta-Tricalcium Phosphate: An In Vivo Assessment" in Applied Sciences, 14, no. 1 (2024):16,
https://doi.org/10.3390/app14010016 . .

TY  - JOUR
AU  - Jakovljević, Aleksandar
AU  - Andrić, Miroslav
AU  - Miletić, Maja
AU  - Beljic-Ivanović, Katarina
AU  - Knežević, Aleksandra
AU  - Mojsilović, Slavko
AU  - Milasin, Jelena
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/741
AB  - Chronic inflammatory processes in periapical tissues caused by etiological agents of endodontic origin lead to apical periodontitis. Apart from bacteria, two herpesviruses, Epstein-Barr virus (EBV) and Human cytomegalovirus (HCMV) are recognized as putative pathogens in apical periodontitis. Although previous reports suggest the involvement of EBV in the pathogenesis of apical periodontitis, its exact role in periapical bone resorption has not yet been fully elucidated. We hypothesize that EBV infection in apical periodontitis is capable of inducing periapical bone resorption via stimulation of reactive oxygen species (ROS) overproduction. Increased levels of ROS induce expression of receptor activator of nuclear factor kappa B (NF-kappa B) ligand (RANKL). RANKL binding to receptor activator of nuclear factor kappa B (RANK) present on the surface of preosteoclasts induces their maturation and activation which consequently leads to bone resorption. The potential benefit of antiviral and antioxidant-based therapies in periapical bone resorption treatment remains to be assessed.
PB  - Churchill Livingstone, Edinburgh
T2  - Medical Hypotheses
T1  - Epstein-Barr virus infection induces bone resorption in apical periodontitis via increased production of reactive oxygen species
EP  - 42
SP  - 40
VL  - 94
DO  - 10.1016/j.mehy.2016.06.020
ER  - 

@article{
author = "Jakovljević, Aleksandar and Andrić, Miroslav and Miletić, Maja and Beljic-Ivanović, Katarina and Knežević, Aleksandra and Mojsilović, Slavko and Milasin, Jelena",
year = "2016",
abstract = "Chronic inflammatory processes in periapical tissues caused by etiological agents of endodontic origin lead to apical periodontitis. Apart from bacteria, two herpesviruses, Epstein-Barr virus (EBV) and Human cytomegalovirus (HCMV) are recognized as putative pathogens in apical periodontitis. Although previous reports suggest the involvement of EBV in the pathogenesis of apical periodontitis, its exact role in periapical bone resorption has not yet been fully elucidated. We hypothesize that EBV infection in apical periodontitis is capable of inducing periapical bone resorption via stimulation of reactive oxygen species (ROS) overproduction. Increased levels of ROS induce expression of receptor activator of nuclear factor kappa B (NF-kappa B) ligand (RANKL). RANKL binding to receptor activator of nuclear factor kappa B (RANK) present on the surface of preosteoclasts induces their maturation and activation which consequently leads to bone resorption. The potential benefit of antiviral and antioxidant-based therapies in periapical bone resorption treatment remains to be assessed.",
publisher = "Churchill Livingstone, Edinburgh",
journal = "Medical Hypotheses",
title = "Epstein-Barr virus infection induces bone resorption in apical periodontitis via increased production of reactive oxygen species",
pages = "42-40",
volume = "94",
doi = "10.1016/j.mehy.2016.06.020"
}

Jakovljević, A., Andrić, M., Miletić, M., Beljic-Ivanović, K., Knežević, A., Mojsilović, S.,& Milasin, J.. (2016). Epstein-Barr virus infection induces bone resorption in apical periodontitis via increased production of reactive oxygen species. in Medical Hypotheses
Churchill Livingstone, Edinburgh., 94, 40-42.
https://doi.org/10.1016/j.mehy.2016.06.020

Jakovljević A, Andrić M, Miletić M, Beljic-Ivanović K, Knežević A, Mojsilović S, Milasin J. Epstein-Barr virus infection induces bone resorption in apical periodontitis via increased production of reactive oxygen species. in Medical Hypotheses. 2016;94:40-42.
doi:10.1016/j.mehy.2016.06.020 .

Jakovljević, Aleksandar, Andrić, Miroslav, Miletić, Maja, Beljic-Ivanović, Katarina, Knežević, Aleksandra, Mojsilović, Slavko, Milasin, Jelena, "Epstein-Barr virus infection induces bone resorption in apical periodontitis via increased production of reactive oxygen species" in Medical Hypotheses, 94 (2016):40-42,
https://doi.org/10.1016/j.mehy.2016.06.020 . .