TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Betić, Nikola
AU  - Karabasil, Neđeljko
AU  - Ćirković, Vladimir
AU  - Đurković-Đaković, Olgica
AU  - Klun, Ivana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1328
AB  - As pork is an important source for Toxoplasma gondii infection, we have analyzed T. gondii genotypes and toxoplasmosis prevalence in pigs in Serbia in the context of production statistics and economics to assess the specific risk to public health. Genotyping was performed using MnPCR-RFLP; T. gondii-specific IgG antibodies were detected using a modified agglutination test (MAT); and statistical data were extracted from official records and provided by government authorities. The results indicate that, from 2006 to 2021, the median number of annually slaughtered pigs was 5.6 million, yet only 36.1% were processed by abattoirs. The remainder were “backyard pigs” slaughtered on family farms and homesteads. Toxoplasmosis seroprevalence in market-weight (MW) pigs prior to 2006 was 15.2%, and was 15.1% in 2019. The seroprevalence in owned city cats, likely infected by livestock meat, was 33.2%. ToxoDB#1 was identified in pig tissues. The results indicate that backyard pigs are the backbone of the industry and provide as much as 60% of the pork in Serbia. The seroprevalence in pigs and city cats shows that farms are reservoirs for the parasite. Thus, innovative means of reducing T. gondii infection designed with backyard farmers in mind are needed to reduce the risk to public health.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Microorganisms
T2  - Microorganisms
T1  - A Pork Industry in the Backyard: An Analysis of Toxoplasma gondii Infection in Serbia’s Pigs
IS  - 7
SP  - 1857
VL  - 11
DO  - 10.3390/microorganisms11071857
ER  - 

@article{
author = "Uzelac, Aleksandra and Betić, Nikola and Karabasil, Neđeljko and Ćirković, Vladimir and Đurković-Đaković, Olgica and Klun, Ivana",
year = "2023",
abstract = "As pork is an important source for Toxoplasma gondii infection, we have analyzed T. gondii genotypes and toxoplasmosis prevalence in pigs in Serbia in the context of production statistics and economics to assess the specific risk to public health. Genotyping was performed using MnPCR-RFLP; T. gondii-specific IgG antibodies were detected using a modified agglutination test (MAT); and statistical data were extracted from official records and provided by government authorities. The results indicate that, from 2006 to 2021, the median number of annually slaughtered pigs was 5.6 million, yet only 36.1% were processed by abattoirs. The remainder were “backyard pigs” slaughtered on family farms and homesteads. Toxoplasmosis seroprevalence in market-weight (MW) pigs prior to 2006 was 15.2%, and was 15.1% in 2019. The seroprevalence in owned city cats, likely infected by livestock meat, was 33.2%. ToxoDB#1 was identified in pig tissues. The results indicate that backyard pigs are the backbone of the industry and provide as much as 60% of the pork in Serbia. The seroprevalence in pigs and city cats shows that farms are reservoirs for the parasite. Thus, innovative means of reducing T. gondii infection designed with backyard farmers in mind are needed to reduce the risk to public health.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Microorganisms, Microorganisms",
title = "A Pork Industry in the Backyard: An Analysis of Toxoplasma gondii Infection in Serbia’s Pigs",
number = "7",
pages = "1857",
volume = "11",
doi = "10.3390/microorganisms11071857"
}

Uzelac, A., Betić, N., Karabasil, N., Ćirković, V., Đurković-Đaković, O.,& Klun, I.. (2023). A Pork Industry in the Backyard: An Analysis of Toxoplasma gondii Infection in Serbia’s Pigs. in Microorganisms
Multidisciplinary Digital Publishing Institute (MDPI)., 11(7), 1857.
https://doi.org/10.3390/microorganisms11071857

Uzelac A, Betić N, Karabasil N, Ćirković V, Đurković-Đaković O, Klun I. A Pork Industry in the Backyard: An Analysis of Toxoplasma gondii Infection in Serbia’s Pigs. in Microorganisms. 2023;11(7):1857.
doi:10.3390/microorganisms11071857 .

Uzelac, Aleksandra, Betić, Nikola, Karabasil, Neđeljko, Ćirković, Vladimir, Đurković-Đaković, Olgica, Klun, Ivana, "A Pork Industry in the Backyard: An Analysis of Toxoplasma gondii Infection in Serbia’s Pigs" in Microorganisms, 11, no. 7 (2023):1857,
https://doi.org/10.3390/microorganisms11071857 . .

TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Betić, Nikola
AU  - Karabasil, Nedjeljko
AU  - Ćirković, Vladimir
AU  - Đurković-Đaković, Olgica
AU  - Klun, Ivana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1402
AB  - Toxoplasma gondii is a common zoonotic intracellular parasite in livestock raised forhuman consumption and is a public health concern. The mode of transmission is ingestion,and meat is considered to be a major vehicle for human and animal infection. AsT. gondii is environmentally transmissible, other important vehicles in particular foranimals include vegetation, soil and water. The seroprevalence of T. gondii infection inpigs is Serbia has been determined in several studies over the past two decades. It hasbeen established that it varies considerably, primarily based on husbandry, with strictlyto mostly indoor animals having a lower prevalence (below 20%) than animals raisedoutdoors, where prevalence exceeds 60%. Experimental data suggests that differentgenotypes of the parasite vary in virulence, but the significance of virulence in terms ofpathology and disease manifestations is still being investigated. Genotypes of T. gondiiisolated from pig tissues in Serbia to date are ToxoDB#1 (archetype II) and ToxoDB#2(archetype III). Archetype II is predominant and, based on historical reports and recentfindings, low to intermediately virulent. The virulence phenotype and mechanisms ofarchetype III, however, have not been extensively studied, but recent data suggests thatits virulence may vary considerably. This review will also summarize the current knowledgeregarding the virulence of archetypes II and III and evaluate it in the context ofthe pig host.
PB  - Institute of Meat Hygiene and Technology
T2  - Meat Technology
T1  - Toxoplasma gondii infection in pigs in Serbia
EP  - 88
IS  - 2
SP  - 82
VL  - 64
DO  - 10.18485/meattech.2023.64.2.14
ER  - 

@article{
author = "Uzelac, Aleksandra and Betić, Nikola and Karabasil, Nedjeljko and Ćirković, Vladimir and Đurković-Đaković, Olgica and Klun, Ivana",
year = "2023",
abstract = "Toxoplasma gondii is a common zoonotic intracellular parasite in livestock raised forhuman consumption and is a public health concern. The mode of transmission is ingestion,and meat is considered to be a major vehicle for human and animal infection. AsT. gondii is environmentally transmissible, other important vehicles in particular foranimals include vegetation, soil and water. The seroprevalence of T. gondii infection inpigs is Serbia has been determined in several studies over the past two decades. It hasbeen established that it varies considerably, primarily based on husbandry, with strictlyto mostly indoor animals having a lower prevalence (below 20%) than animals raisedoutdoors, where prevalence exceeds 60%. Experimental data suggests that differentgenotypes of the parasite vary in virulence, but the significance of virulence in terms ofpathology and disease manifestations is still being investigated. Genotypes of T. gondiiisolated from pig tissues in Serbia to date are ToxoDB#1 (archetype II) and ToxoDB#2(archetype III). Archetype II is predominant and, based on historical reports and recentfindings, low to intermediately virulent. The virulence phenotype and mechanisms ofarchetype III, however, have not been extensively studied, but recent data suggests thatits virulence may vary considerably. This review will also summarize the current knowledgeregarding the virulence of archetypes II and III and evaluate it in the context ofthe pig host.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology",
title = "Toxoplasma gondii infection in pigs in Serbia",
pages = "88-82",
number = "2",
volume = "64",
doi = "10.18485/meattech.2023.64.2.14"
}

Uzelac, A., Betić, N., Karabasil, N., Ćirković, V., Đurković-Đaković, O.,& Klun, I.. (2023). Toxoplasma gondii infection in pigs in Serbia. in Meat Technology
Institute of Meat Hygiene and Technology., 64(2), 82-88.
https://doi.org/10.18485/meattech.2023.64.2.14

Uzelac A, Betić N, Karabasil N, Ćirković V, Đurković-Đaković O, Klun I. Toxoplasma gondii infection in pigs in Serbia. in Meat Technology. 2023;64(2):82-88.
doi:10.18485/meattech.2023.64.2.14 .

Uzelac, Aleksandra, Betić, Nikola, Karabasil, Nedjeljko, Ćirković, Vladimir, Đurković-Đaković, Olgica, Klun, Ivana, "Toxoplasma gondii infection in pigs in Serbia" in Meat Technology, 64, no. 2 (2023):82-88,
https://doi.org/10.18485/meattech.2023.64.2.14 . .

TY  - JOUR
AU  - Čokić, Vladan
AU  - Popovska, Zorana
AU  - Lijeskić, Olivera
AU  - Šabić, Ljiljana
AU  - Đurković-Đaković, Olgica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1317
AB  - Older people in nursing homes (NH) have been hit particularly hard by the COVID-19 pandemic. We conducted a retrospective study of three outbreaks of COVID-19, occurring during the waves of the initial pre-Alpha, Delta and Omicron SARS-CoV-2 variants, in one NH in suburban Belgrade, Serbia. All staff and 95% residents were vaccinated in February 2021, mostly with BBIBP-CorV, and two thirds were boosted with a third dose in August 2021. COVID-19 was diagnosed by positive PCR and/or antigen test. After the first outbreak, 80 affected individuals were tested for SARS-CoV-2 specific antibodies. The first outbreak involved 64/126 (50.8%) residents and 45/64 (70.3%) staff, the second 22/75 (29.3%) residents and 3/40 (7.5%) staff, and the third involved 36/110 (32.7%) residents and 19/56 (33.9%) staff. Clinical presentation ranged from asymptomatic to severe, with severe cases referred to hospital ICUs. Deaths occurred only in residents, and the case fatality rate was 31.2%, 9.1% and 0%, respectively in outbreaks 1, 2 and 3. Specific IgG antibodies were detected in all 35 residents and 44 of the 45 staff, and higher IgG levels were detected in the residents (417.3±273.5) than in the staff (201.9±192.9, p<0.0001) despite a double difference in age (79.0±7.4 vs. 40.1±11.5 years). Outbreaks 2 and 3 involved four and 23 breakthrough infections, respectively. Older individuals mounted a good immunological response to SARS-CoV-2 infection and vaccination, which prevented significant mortality and severe morbidity in the subsequent outbreaks, despite a significant number of breakthrough infections.
PB  - International Society on Aging and Disease
T2  - Aging and disease
T1  - Three Outbreaks of COVID-19 in a Single Nursing Home over Two Years of the SARS-CoV-2 Pandemic
EP  - 111
IS  - 1
SP  - 99
VL  - 14
DO  - 10.14336/AD.2022.0624
ER  - 

@article{
author = "Čokić, Vladan and Popovska, Zorana and Lijeskić, Olivera and Šabić, Ljiljana and Đurković-Đaković, Olgica",
year = "2023",
abstract = "Older people in nursing homes (NH) have been hit particularly hard by the COVID-19 pandemic. We conducted a retrospective study of three outbreaks of COVID-19, occurring during the waves of the initial pre-Alpha, Delta and Omicron SARS-CoV-2 variants, in one NH in suburban Belgrade, Serbia. All staff and 95% residents were vaccinated in February 2021, mostly with BBIBP-CorV, and two thirds were boosted with a third dose in August 2021. COVID-19 was diagnosed by positive PCR and/or antigen test. After the first outbreak, 80 affected individuals were tested for SARS-CoV-2 specific antibodies. The first outbreak involved 64/126 (50.8%) residents and 45/64 (70.3%) staff, the second 22/75 (29.3%) residents and 3/40 (7.5%) staff, and the third involved 36/110 (32.7%) residents and 19/56 (33.9%) staff. Clinical presentation ranged from asymptomatic to severe, with severe cases referred to hospital ICUs. Deaths occurred only in residents, and the case fatality rate was 31.2%, 9.1% and 0%, respectively in outbreaks 1, 2 and 3. Specific IgG antibodies were detected in all 35 residents and 44 of the 45 staff, and higher IgG levels were detected in the residents (417.3±273.5) than in the staff (201.9±192.9, p<0.0001) despite a double difference in age (79.0±7.4 vs. 40.1±11.5 years). Outbreaks 2 and 3 involved four and 23 breakthrough infections, respectively. Older individuals mounted a good immunological response to SARS-CoV-2 infection and vaccination, which prevented significant mortality and severe morbidity in the subsequent outbreaks, despite a significant number of breakthrough infections.",
publisher = "International Society on Aging and Disease",
journal = "Aging and disease",
title = "Three Outbreaks of COVID-19 in a Single Nursing Home over Two Years of the SARS-CoV-2 Pandemic",
pages = "111-99",
number = "1",
volume = "14",
doi = "10.14336/AD.2022.0624"
}

Čokić, V., Popovska, Z., Lijeskić, O., Šabić, L.,& Đurković-Đaković, O.. (2023). Three Outbreaks of COVID-19 in a Single Nursing Home over Two Years of the SARS-CoV-2 Pandemic. in Aging and disease
International Society on Aging and Disease., 14(1), 99-111.
https://doi.org/10.14336/AD.2022.0624

Čokić V, Popovska Z, Lijeskić O, Šabić L, Đurković-Đaković O. Three Outbreaks of COVID-19 in a Single Nursing Home over Two Years of the SARS-CoV-2 Pandemic. in Aging and disease. 2023;14(1):99-111.
doi:10.14336/AD.2022.0624 .

Čokić, Vladan, Popovska, Zorana, Lijeskić, Olivera, Šabić, Ljiljana, Đurković-Đaković, Olgica, "Three Outbreaks of COVID-19 in a Single Nursing Home over Two Years of the SARS-CoV-2 Pandemic" in Aging and disease, 14, no. 1 (2023):99-111,
https://doi.org/10.14336/AD.2022.0624 . .

TY  - JOUR
AU  - Kokić, Zoran
AU  - Kon, Predrag
AU  - Đurković-Đaković, Olgica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1306
AB  - The course of the COVID-19 pandemic has been critically altered by the availability of vaccines. To assess the risk of COVID-19 in the vaccinated, as compared to the unvaccinated population, as well as the comparative effectiveness of the BBIBP-CorV (Sinopharm), BNT162b2 (Pfizer/BioNTech), Gam-COVID-Vac (Sputnik V) and ChAdOx1 (AstraZeneca) vaccines in the prevention of clinical infection, we carried out a retrospective study of the incidence of clinical COVID-19 in the Belgrade city municipality of Voždovac among both vaccinated and unvaccinated individuals during a 4-month period between 1 July and 31 October 2021. The study included all individuals with a symptomatic infection confirmed by a positive PCR and/or antigen test. Only those who received two vaccine doses were considered as vaccinated. The results showed that of the Voždovac population of 169,567, a total of 81,447 (48%) individuals were vaccinated by the end of the study. Vaccination coverage increased with age, ranging from 1.06% in those below age 18, to even 78.8% in those above 65 years of age. More than one half (57.5%) of all those vaccinated received BBIBP-CorV, while 25.2% received BNT162b2, 11.7% Gam-COVID-Vac and 5.6% ChAdOx1. The overall risk of infection of the vaccinated vs. the unvaccinated was 0.53 (95% CI 0.45–0.61). Compared to the incidence of COVID-19 of 8.05 per 1000 in the unvaccinated population, the relative risk in the vaccinated was 0.35 (95% CI 0.3–0.41). The overall VE was 65%, differing widely among age groups and by vaccine. VE was 79% for BNT162b2, 62% for BBIBP-CorV, 60% for ChAdOx1 and 54% for Gam-COVID-Vac. The VE for BBIBP-CorV and BNT162b2 increased with age. The obtained results demonstrate a significant overall effectiveness of anti-COVID-19 vaccination, which, however, varied significantly among the analyzed vaccines, and was the highest for BNT162b2.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Vaccines
T1  - Effectiveness of Vaccination in Preventing COVID-19: A Community Study Comparing Four Vaccines
IS  - 3
SP  - 544
VL  - 11
DO  - 10.3390/vaccines11030544
ER  - 

@article{
author = "Kokić, Zoran and Kon, Predrag and Đurković-Đaković, Olgica",
year = "2023",
abstract = "The course of the COVID-19 pandemic has been critically altered by the availability of vaccines. To assess the risk of COVID-19 in the vaccinated, as compared to the unvaccinated population, as well as the comparative effectiveness of the BBIBP-CorV (Sinopharm), BNT162b2 (Pfizer/BioNTech), Gam-COVID-Vac (Sputnik V) and ChAdOx1 (AstraZeneca) vaccines in the prevention of clinical infection, we carried out a retrospective study of the incidence of clinical COVID-19 in the Belgrade city municipality of Voždovac among both vaccinated and unvaccinated individuals during a 4-month period between 1 July and 31 October 2021. The study included all individuals with a symptomatic infection confirmed by a positive PCR and/or antigen test. Only those who received two vaccine doses were considered as vaccinated. The results showed that of the Voždovac population of 169,567, a total of 81,447 (48%) individuals were vaccinated by the end of the study. Vaccination coverage increased with age, ranging from 1.06% in those below age 18, to even 78.8% in those above 65 years of age. More than one half (57.5%) of all those vaccinated received BBIBP-CorV, while 25.2% received BNT162b2, 11.7% Gam-COVID-Vac and 5.6% ChAdOx1. The overall risk of infection of the vaccinated vs. the unvaccinated was 0.53 (95% CI 0.45–0.61). Compared to the incidence of COVID-19 of 8.05 per 1000 in the unvaccinated population, the relative risk in the vaccinated was 0.35 (95% CI 0.3–0.41). The overall VE was 65%, differing widely among age groups and by vaccine. VE was 79% for BNT162b2, 62% for BBIBP-CorV, 60% for ChAdOx1 and 54% for Gam-COVID-Vac. The VE for BBIBP-CorV and BNT162b2 increased with age. The obtained results demonstrate a significant overall effectiveness of anti-COVID-19 vaccination, which, however, varied significantly among the analyzed vaccines, and was the highest for BNT162b2.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Vaccines",
title = "Effectiveness of Vaccination in Preventing COVID-19: A Community Study Comparing Four Vaccines",
number = "3",
pages = "544",
volume = "11",
doi = "10.3390/vaccines11030544"
}

Kokić, Z., Kon, P.,& Đurković-Đaković, O.. (2023). Effectiveness of Vaccination in Preventing COVID-19: A Community Study Comparing Four Vaccines. in Vaccines
Multidisciplinary Digital Publishing Institute (MDPI)., 11(3), 544.
https://doi.org/10.3390/vaccines11030544

Kokić Z, Kon P, Đurković-Đaković O. Effectiveness of Vaccination in Preventing COVID-19: A Community Study Comparing Four Vaccines. in Vaccines. 2023;11(3):544.
doi:10.3390/vaccines11030544 .

Kokić, Zoran, Kon, Predrag, Đurković-Đaković, Olgica, "Effectiveness of Vaccination in Preventing COVID-19: A Community Study Comparing Four Vaccines" in Vaccines, 11, no. 3 (2023):544,
https://doi.org/10.3390/vaccines11030544 . .

TY  - JOUR
AU  - Stopić, Milena
AU  - Štajner, Tijana
AU  - Marković-Denić, Ljiljana
AU  - Nikolić, Vladimir
AU  - Đilas, Iva
AU  - Jovanović Srzentić, Snežana
AU  - Đurković-Đaković, Olgica
AU  - Bobić, Branko
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1224
AB  - Toxoplasmosis is a globally distributed parasitic zoonosis, affecting approximately one third of the human population. Epidemiological studies on toxoplasmosis conducted in Serbia so far have been focused on women of childbearing age, without a clear insight into the prevalence in the general population. We conducted a cross-sectional study in a representative sample of the healthy adult population consisting of 1095 blood donors of both genders to establish the prevalence and risk factors for Toxoplasma gondii infection. Data on the demographic and clinical characteristics of all study participants, as well as on their lifestyle habits, were collected by means of a questionnaire. The overall prevalence of infection was 20.5% (224/1095) and the avidity of the specific IgG antibodies detected was high in a vast majority of the seropositive donors (98.2%). Interestingly, the remaining 1.8% of the specific IgG positive samples were of borderline avidity (4/224), in complete absence of specific IgM. The multivariate logistic regression analysis showed that independent risk factors included age (from OR (95% CI) 1.9 (1.13–3.28) in the 30–39 age group, to 6.8 (3.27–14.24) in the age group of >60 years), suburban living (OR (95% CI) 2.2 (1.43–3.34)) and contact with soil (OR (95% CI) 1.4 (1.01–1.94)). This first large-scale study on toxoplasmosis in the general population in Serbia shows the lowest prevalence ever reported in this country. Moreover, the novel perspective on risk factors provides an updated basis for future prevention programs.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Microorganisms
T1  - Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors
IS  - 3
SP  - 492
VL  - 10
DO  - 10.3390/microorganisms10030492
ER  - 

@article{
author = "Stopić, Milena and Štajner, Tijana and Marković-Denić, Ljiljana and Nikolić, Vladimir and Đilas, Iva and Jovanović Srzentić, Snežana and Đurković-Đaković, Olgica and Bobić, Branko",
year = "2022",
abstract = "Toxoplasmosis is a globally distributed parasitic zoonosis, affecting approximately one third of the human population. Epidemiological studies on toxoplasmosis conducted in Serbia so far have been focused on women of childbearing age, without a clear insight into the prevalence in the general population. We conducted a cross-sectional study in a representative sample of the healthy adult population consisting of 1095 blood donors of both genders to establish the prevalence and risk factors for Toxoplasma gondii infection. Data on the demographic and clinical characteristics of all study participants, as well as on their lifestyle habits, were collected by means of a questionnaire. The overall prevalence of infection was 20.5% (224/1095) and the avidity of the specific IgG antibodies detected was high in a vast majority of the seropositive donors (98.2%). Interestingly, the remaining 1.8% of the specific IgG positive samples were of borderline avidity (4/224), in complete absence of specific IgM. The multivariate logistic regression analysis showed that independent risk factors included age (from OR (95% CI) 1.9 (1.13–3.28) in the 30–39 age group, to 6.8 (3.27–14.24) in the age group of >60 years), suburban living (OR (95% CI) 2.2 (1.43–3.34)) and contact with soil (OR (95% CI) 1.4 (1.01–1.94)). This first large-scale study on toxoplasmosis in the general population in Serbia shows the lowest prevalence ever reported in this country. Moreover, the novel perspective on risk factors provides an updated basis for future prevention programs.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Microorganisms",
title = "Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors",
number = "3",
pages = "492",
volume = "10",
doi = "10.3390/microorganisms10030492"
}

Stopić, M., Štajner, T., Marković-Denić, L., Nikolić, V., Đilas, I., Jovanović Srzentić, S., Đurković-Đaković, O.,& Bobić, B.. (2022). Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors. in Microorganisms
Multidisciplinary Digital Publishing Institute (MDPI)., 10(3), 492.
https://doi.org/10.3390/microorganisms10030492

Stopić M, Štajner T, Marković-Denić L, Nikolić V, Đilas I, Jovanović Srzentić S, Đurković-Đaković O, Bobić B. Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors. in Microorganisms. 2022;10(3):492.
doi:10.3390/microorganisms10030492 .

Stopić, Milena, Štajner, Tijana, Marković-Denić, Ljiljana, Nikolić, Vladimir, Đilas, Iva, Jovanović Srzentić, Snežana, Đurković-Đaković, Olgica, Bobić, Branko, "Epidemiology of Toxoplasmosis in SERBIA: A Cross-Sectional Study on Blood Donors" in Microorganisms, 10, no. 3 (2022):492,
https://doi.org/10.3390/microorganisms10030492 . .

TY  - JOUR
AU  - Betić, Nikola
AU  - Karabasil, Neđeljko
AU  - Đurković-Đaković, Olgica
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Branković Lazić, Ivana
AU  - Đorđević, Vesna
AU  - Klun, Ivana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1240
AB  - Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.
T2  - Microorganisms
T1  - Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures
IS  - 5
SP  - 1069
VL  - 10
DO  - 10.3390/microorganisms10051069
ER  - 

@article{
author = "Betić, Nikola and Karabasil, Neđeljko and Đurković-Đaković, Olgica and Ćirković, Vladimir and Bobić, Branko and Branković Lazić, Ivana and Đorđević, Vesna and Klun, Ivana",
year = "2022",
abstract = "Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.",
journal = "Microorganisms",
title = "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures",
number = "5",
pages = "1069",
volume = "10",
doi = "10.3390/microorganisms10051069"
}

Betić, N., Karabasil, N., Đurković-Đaković, O., Ćirković, V., Bobić, B., Branković Lazić, I., Đorđević, V.,& Klun, I.. (2022). Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms, 10(5), 1069.
https://doi.org/10.3390/microorganisms10051069

Betić N, Karabasil N, Đurković-Đaković O, Ćirković V, Bobić B, Branković Lazić I, Đorđević V, Klun I. Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms. 2022;10(5):1069.
doi:10.3390/microorganisms10051069 .

Betić, Nikola, Karabasil, Neđeljko, Đurković-Đaković, Olgica, Ćirković, Vladimir, Bobić, Branko, Branković Lazić, Ivana, Đorđević, Vesna, Klun, Ivana, "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures" in Microorganisms, 10, no. 5 (2022):1069,
https://doi.org/10.3390/microorganisms10051069 . .

TY  - CONF
AU  - Čokić, Vladan
AU  - Popovska, Zorana
AU  - Lijeskić, Olivera
AU  - Šabić, Ljiljana
AU  - Đurković-Đaković, Olgica
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1455
AB  - Background: Over the two years of the COVID-19 pandemic, the elderly in nursing
homes (NH) have been hit particularly hard.
Methods: We conducted a retrospective study of 3 episodes of COVID-19 in one NH in
suburban Belgrade, Serbia, at the time of pre-alpha (Nov 2020), delta (Nov 2021) and
omicron (Jan 2022) variants of SARS-CoV-2. All staff and 95% of residents were vaccinated
in the early spring of 2021, with BBIBP-CorV, Gam-COVID-Vac and BNT162b2
vaccines. COVID-19 was diagnosed by positive PCR and/or antigen test. Specific IgG
antibodies against SARS-CoV-2 S glycoprotein RBD were assessed by ELISA.
Results: The 3 episodes involved a total of 188 infections. The first involved 65/126
(51.9%) residents and 44/64 (68.7%) staff, the second 22/75 (29.3%) residents and 3/40
(7.5%) staff, and the third 36/110 (32.7%) residents and 18/56 (32.1%) staff. Clinical presentation
ranged from asymptomatic to severe, with severe cases being referred to
hospital ICUs. Mortality per episode was 19.8%, 2.7% and 0%, respectively, and involved
residents only. After the first episode, all 36 examined residents and 43 of the
44 staff had specific antibodies. Interestingly, higher levels (20.45±13.27) were detected
in the residents than in the staff (9.74±9.52) (p<0.001) despite a double difference
in age (79.6±7.48 vs. 40.8±11.43) (p<0.001). Episodes 2 and 3 involved 4 (1 resident, 3
staff) and 22 (13 residents, 9 staff) breakthrough infections.
Conclusions: Elderly individuals mounted a good immunological response to the
vaccines, which prevented significant mortality in the next episodes, despite a significant
number of omicron-induced breakthrough infections.
PB  - Belgrade: MEDAPP Association
C3  - The First World Conference Fighting COVID-19 Pandemic - Health Challenges, 26-28 March 2022, Belgrade, Serbia -Abstract book
T1  - Three Episodes of COVID-19 in a Nursing Home in Belgrade, Serbia
EP  - 94
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1455
ER  - 

@conference{
author = "Čokić, Vladan and Popovska, Zorana and Lijeskić, Olivera and Šabić, Ljiljana and Đurković-Đaković, Olgica",
year = "2022",
abstract = "Background: Over the two years of the COVID-19 pandemic, the elderly in nursing
homes (NH) have been hit particularly hard.
Methods: We conducted a retrospective study of 3 episodes of COVID-19 in one NH in
suburban Belgrade, Serbia, at the time of pre-alpha (Nov 2020), delta (Nov 2021) and
omicron (Jan 2022) variants of SARS-CoV-2. All staff and 95% of residents were vaccinated
in the early spring of 2021, with BBIBP-CorV, Gam-COVID-Vac and BNT162b2
vaccines. COVID-19 was diagnosed by positive PCR and/or antigen test. Specific IgG
antibodies against SARS-CoV-2 S glycoprotein RBD were assessed by ELISA.
Results: The 3 episodes involved a total of 188 infections. The first involved 65/126
(51.9%) residents and 44/64 (68.7%) staff, the second 22/75 (29.3%) residents and 3/40
(7.5%) staff, and the third 36/110 (32.7%) residents and 18/56 (32.1%) staff. Clinical presentation
ranged from asymptomatic to severe, with severe cases being referred to
hospital ICUs. Mortality per episode was 19.8%, 2.7% and 0%, respectively, and involved
residents only. After the first episode, all 36 examined residents and 43 of the
44 staff had specific antibodies. Interestingly, higher levels (20.45±13.27) were detected
in the residents than in the staff (9.74±9.52) (p<0.001) despite a double difference
in age (79.6±7.48 vs. 40.8±11.43) (p<0.001). Episodes 2 and 3 involved 4 (1 resident, 3
staff) and 22 (13 residents, 9 staff) breakthrough infections.
Conclusions: Elderly individuals mounted a good immunological response to the
vaccines, which prevented significant mortality in the next episodes, despite a significant
number of omicron-induced breakthrough infections.",
publisher = "Belgrade: MEDAPP Association",
journal = "The First World Conference Fighting COVID-19 Pandemic - Health Challenges, 26-28 March 2022, Belgrade, Serbia -Abstract book",
title = "Three Episodes of COVID-19 in a Nursing Home in Belgrade, Serbia",
pages = "94-94",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1455"
}

Čokić, V., Popovska, Z., Lijeskić, O., Šabić, L.,& Đurković-Đaković, O.. (2022). Three Episodes of COVID-19 in a Nursing Home in Belgrade, Serbia. in The First World Conference Fighting COVID-19 Pandemic - Health Challenges, 26-28 March 2022, Belgrade, Serbia -Abstract book
Belgrade: MEDAPP Association., 94-94.
https://hdl.handle.net/21.15107/rcub_rimi_1455

Čokić V, Popovska Z, Lijeskić O, Šabić L, Đurković-Đaković O. Three Episodes of COVID-19 in a Nursing Home in Belgrade, Serbia. in The First World Conference Fighting COVID-19 Pandemic - Health Challenges, 26-28 March 2022, Belgrade, Serbia -Abstract book. 2022;:94-94.
https://hdl.handle.net/21.15107/rcub_rimi_1455 .

Čokić, Vladan, Popovska, Zorana, Lijeskić, Olivera, Šabić, Ljiljana, Đurković-Đaković, Olgica, "Three Episodes of COVID-19 in a Nursing Home in Belgrade, Serbia" in The First World Conference Fighting COVID-19 Pandemic - Health Challenges, 26-28 March 2022, Belgrade, Serbia -Abstract book (2022):94-94,
https://hdl.handle.net/21.15107/rcub_rimi_1455 .

Opsenica, I., Selaković, M., Tot, M., Verbić, T., Srbljanović, J., Štajner, T., Đurković-Đaković, O.,& Šolaja, B.. (2021). New 4-aminoquinolines as moderate inhibitors of P. falciparum malaria. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 86(2), 115-123.
https://doi.org/10.2298/JSC201225005O

Opsenica I, Selaković M, Tot M, Verbić T, Srbljanović J, Štajner T, Đurković-Đaković O, Šolaja B. New 4-aminoquinolines as moderate inhibitors of P. falciparum malaria. in Journal of the Serbian Chemical Society. 2021;86(2):115-123.
doi:10.2298/JSC201225005O .

Opsenica, Igor, Selaković, Milica, Tot, Mikloš, Verbić, Tatjana, Srbljanović, Jelena, Štajner, Tijana, Đurković-Đaković, Olgica, Šolaja, Bogdan, "New 4-aminoquinolines as moderate inhibitors of P. falciparum malaria" in Journal of the Serbian Chemical Society, 86, no. 2 (2021):115-123,
https://doi.org/10.2298/JSC201225005O . .

TY  - JOUR
AU  - Guegan, Helene
AU  - Štajner, Tijana
AU  - Bobić, Branko
AU  - Press, Cindy
AU  - Olariu, Rares T.
AU  - Olson, Kjerstie
AU  - Srbljanović, Jelena
AU  - Montoya, Jose G.
AU  - Đurković-Đaković, Olgica
AU  - Robert-Gangneux, Florence
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1129
AB  - Neonatal diagnosis of congenital toxoplasmosis is based on a combination of serological and molecular tests. Maternal screening and treatment differ according to national policies and may impact the sensitivity of diagnostic methods in infants at birth. In this multicenter study, 115 neonates born to 61 treated (53%) and 54 (47%) untreated women were retrospectively included in three centers (France, Serbia, and the United States) to assess the impact of maternal anti-Toxoplasma treatment on the performance of neonatal workup at birth (neosynthesized anti-Toxoplasma IgM, IgA, and IgG and quantitative PCR [qPCR]) using univariate and multivariate approaches. Independently of the time of maternal seroconversion, the serological techniques were impacted differently by maternal treatment. The detection of IgM by immunosorbent agglutination assay (ISAGA) and Western blotting (WB) dropped from 90.7% and 88.2% in untreated neonates to 533% and 51.9% in treated neonates (P  lt  0.05), whereas IgM enzyme-linked immunosorbent assay (ELISA) and IgA ISAGA were not significantly affected by maternal treatment. A 2-fold reduction in the sensitivity of neosynthesized IgG by WB was also observed in the case of treatment during pregnancy (37.7% versus 82.3%). Interestingly, the effect of treatment was shown to be duration dependent, especially for IgM detection, when the treatment course exceeded 8 weeks, whatever the therapy. The sensitivity of Toxoplasma PCR in blood was also lowered by maternal treatment from 39.1% to 23.2%. These results highlight that anti-Toxoplasma therapy during pregnancy may set back biological evidence of neonatal infection at birth and underline the need for a careful serological follow-up of infants with normal workup.
PB  - Amer Soc Microbiology, Washington
T2  - Journal of Clinical Microbiology
T1  - Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate
IS  - 2
VL  - 59
DO  - 10.1128/JCM.01368-20
ER  - 

@article{
author = "Guegan, Helene and Štajner, Tijana and Bobić, Branko and Press, Cindy and Olariu, Rares T. and Olson, Kjerstie and Srbljanović, Jelena and Montoya, Jose G. and Đurković-Đaković, Olgica and Robert-Gangneux, Florence",
year = "2021",
abstract = "Neonatal diagnosis of congenital toxoplasmosis is based on a combination of serological and molecular tests. Maternal screening and treatment differ according to national policies and may impact the sensitivity of diagnostic methods in infants at birth. In this multicenter study, 115 neonates born to 61 treated (53%) and 54 (47%) untreated women were retrospectively included in three centers (France, Serbia, and the United States) to assess the impact of maternal anti-Toxoplasma treatment on the performance of neonatal workup at birth (neosynthesized anti-Toxoplasma IgM, IgA, and IgG and quantitative PCR [qPCR]) using univariate and multivariate approaches. Independently of the time of maternal seroconversion, the serological techniques were impacted differently by maternal treatment. The detection of IgM by immunosorbent agglutination assay (ISAGA) and Western blotting (WB) dropped from 90.7% and 88.2% in untreated neonates to 533% and 51.9% in treated neonates (P  lt  0.05), whereas IgM enzyme-linked immunosorbent assay (ELISA) and IgA ISAGA were not significantly affected by maternal treatment. A 2-fold reduction in the sensitivity of neosynthesized IgG by WB was also observed in the case of treatment during pregnancy (37.7% versus 82.3%). Interestingly, the effect of treatment was shown to be duration dependent, especially for IgM detection, when the treatment course exceeded 8 weeks, whatever the therapy. The sensitivity of Toxoplasma PCR in blood was also lowered by maternal treatment from 39.1% to 23.2%. These results highlight that anti-Toxoplasma therapy during pregnancy may set back biological evidence of neonatal infection at birth and underline the need for a careful serological follow-up of infants with normal workup.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Journal of Clinical Microbiology",
title = "Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate",
number = "2",
volume = "59",
doi = "10.1128/JCM.01368-20"
}

Guegan, H., Štajner, T., Bobić, B., Press, C., Olariu, R. T., Olson, K., Srbljanović, J., Montoya, J. G., Đurković-Đaković, O.,& Robert-Gangneux, F.. (2021). Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate. in Journal of Clinical Microbiology
Amer Soc Microbiology, Washington., 59(2).
https://doi.org/10.1128/JCM.01368-20

Guegan H, Štajner T, Bobić B, Press C, Olariu RT, Olson K, Srbljanović J, Montoya JG, Đurković-Đaković O, Robert-Gangneux F. Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate. in Journal of Clinical Microbiology. 2021;59(2).
doi:10.1128/JCM.01368-20 .

Guegan, Helene, Štajner, Tijana, Bobić, Branko, Press, Cindy, Olariu, Rares T., Olson, Kjerstie, Srbljanović, Jelena, Montoya, Jose G., Đurković-Đaković, Olgica, Robert-Gangneux, Florence, "Maternal Anti-Toxoplasma Treatment during Pregnancy Is Associated with Reduced Sensitivity of Diagnostic Tests for Congenital Infection in the Neonate" in Journal of Clinical Microbiology, 59, no. 2 (2021),
https://doi.org/10.1128/JCM.01368-20 . .

TY  - JOUR
AU  - Lijeskić, Olivera
AU  - Klun, Ivana
AU  - Stamenov Đaković, Marija
AU  - Gligorić, Nenad
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1168
AB  - Real-life data on the performance of vaccines against SARS-CoV-2 are still limited. We here present the rates of detection and levels of antibodies specific for the SARS-CoV-2 spike protein RBD (receptor binding domain) elicited by four vaccines available in Serbia, including BNT-162b2 (BioNTech/Pfizer), BBIBP-CorV (Sinopharm), Gam-COVID-Vac (Gamaleya Research Institute) and ChAdOx1-S (AstraZeneca), compared with those after documented COVID-19, at 6 weeks and 3 months post first vaccine dose or post-infection. Six weeks post first vaccine dose, specific IgG antibodies were detected in 100% of individuals fully vaccinated with BNT-162b2 (n = 100) and Gam-COVID-Vac (n = 12) and in 81.7% of BBIBP-CorV recipients (n = 148), while one dose of ChAdOx1-S (n = 24) induced specific antibodies in 75%. Antibody levels elicited by BNT-162b2 were higher, while those elicited by BBIBP-CorV were lower, than after SARS-CoV-2 infection. By 3 months post-vaccination, antibody levels decreased but remained ≥20-fold above the cut-off in BNT-162b2 but not in BBIBP-CorV recipients, when an additional 30% were seronegative. For all vaccines, antibody levels were higher in individuals with past COVID-19 than in naïve individuals. A total of twelve new infections occurred within the first 3 months post-vaccination, eight after the first dose of BNT-162b2 and ChAdOx1-S (one each) and BBIBP-CorV (six), and four after full vaccination with BBIBP-CorV, but none required hospitalization.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Vaccines
T1  - Prospective Cohort Study of the Kinetics of Specific Antibodies to SARS-CoV-2 Infection and to Four SARS-CoV-2 Vaccines Available in Serbia, and Vaccine Effectiveness: A 3-Month Interim Report
IS  - 9
SP  - 1031
VL  - 9
DO  - 10.3390/vaccines9091031
ER  - 

@article{
author = "Lijeskić, Olivera and Klun, Ivana and Stamenov Đaković, Marija and Gligorić, Nenad and Štajner, Tijana and Srbljanović, Jelena and Đurković-Đaković, Olgica",
year = "2021",
abstract = "Real-life data on the performance of vaccines against SARS-CoV-2 are still limited. We here present the rates of detection and levels of antibodies specific for the SARS-CoV-2 spike protein RBD (receptor binding domain) elicited by four vaccines available in Serbia, including BNT-162b2 (BioNTech/Pfizer), BBIBP-CorV (Sinopharm), Gam-COVID-Vac (Gamaleya Research Institute) and ChAdOx1-S (AstraZeneca), compared with those after documented COVID-19, at 6 weeks and 3 months post first vaccine dose or post-infection. Six weeks post first vaccine dose, specific IgG antibodies were detected in 100% of individuals fully vaccinated with BNT-162b2 (n = 100) and Gam-COVID-Vac (n = 12) and in 81.7% of BBIBP-CorV recipients (n = 148), while one dose of ChAdOx1-S (n = 24) induced specific antibodies in 75%. Antibody levels elicited by BNT-162b2 were higher, while those elicited by BBIBP-CorV were lower, than after SARS-CoV-2 infection. By 3 months post-vaccination, antibody levels decreased but remained ≥20-fold above the cut-off in BNT-162b2 but not in BBIBP-CorV recipients, when an additional 30% were seronegative. For all vaccines, antibody levels were higher in individuals with past COVID-19 than in naïve individuals. A total of twelve new infections occurred within the first 3 months post-vaccination, eight after the first dose of BNT-162b2 and ChAdOx1-S (one each) and BBIBP-CorV (six), and four after full vaccination with BBIBP-CorV, but none required hospitalization.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Vaccines",
title = "Prospective Cohort Study of the Kinetics of Specific Antibodies to SARS-CoV-2 Infection and to Four SARS-CoV-2 Vaccines Available in Serbia, and Vaccine Effectiveness: A 3-Month Interim Report",
number = "9",
pages = "1031",
volume = "9",
doi = "10.3390/vaccines9091031"
}

Lijeskić, O., Klun, I., Stamenov Đaković, M., Gligorić, N., Štajner, T., Srbljanović, J.,& Đurković-Đaković, O.. (2021). Prospective Cohort Study of the Kinetics of Specific Antibodies to SARS-CoV-2 Infection and to Four SARS-CoV-2 Vaccines Available in Serbia, and Vaccine Effectiveness: A 3-Month Interim Report. in Vaccines
Multidisciplinary Digital Publishing Institute (MDPI)., 9(9), 1031.
https://doi.org/10.3390/vaccines9091031

Lijeskić O, Klun I, Stamenov Đaković M, Gligorić N, Štajner T, Srbljanović J, Đurković-Đaković O. Prospective Cohort Study of the Kinetics of Specific Antibodies to SARS-CoV-2 Infection and to Four SARS-CoV-2 Vaccines Available in Serbia, and Vaccine Effectiveness: A 3-Month Interim Report. in Vaccines. 2021;9(9):1031.
doi:10.3390/vaccines9091031 .

Lijeskić, Olivera, Klun, Ivana, Stamenov Đaković, Marija, Gligorić, Nenad, Štajner, Tijana, Srbljanović, Jelena, Đurković-Đaković, Olgica, "Prospective Cohort Study of the Kinetics of Specific Antibodies to SARS-CoV-2 Infection and to Four SARS-CoV-2 Vaccines Available in Serbia, and Vaccine Effectiveness: A 3-Month Interim Report" in Vaccines, 9, no. 9 (2021):1031,
https://doi.org/10.3390/vaccines9091031 . .

TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Ćirković, Vladimir
AU  - Bauman, Neda
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Lijeskić, Olivera
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1190
AB  - In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.
PB  - MDPI
T2  - Microorganisms
T1  - Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange
IS  - 12
SP  - 2526
VL  - 9
DO  - 10.3390/microorganisms9122526
ER  - 

@article{
author = "Uzelac, Aleksandra and Klun, Ivana and Ćirković, Vladimir and Bauman, Neda and Bobić, Branko and Štajner, Tijana and Srbljanović, Jelena and Lijeskić, Olivera and Đurković-Đaković, Olgica",
year = "2021",
abstract = "In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange",
number = "12",
pages = "2526",
volume = "9",
doi = "10.3390/microorganisms9122526"
}

Uzelac, A., Klun, I., Ćirković, V., Bauman, N., Bobić, B., Štajner, T., Srbljanović, J., Lijeskić, O.,& Đurković-Đaković, O.. (2021). Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms
MDPI., 9(12), 2526.
https://doi.org/10.3390/microorganisms9122526

Uzelac A, Klun I, Ćirković V, Bauman N, Bobić B, Štajner T, Srbljanović J, Lijeskić O, Đurković-Đaković O. Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms. 2021;9(12):2526.
doi:10.3390/microorganisms9122526 .

Uzelac, Aleksandra, Klun, Ivana, Ćirković, Vladimir, Bauman, Neda, Bobić, Branko, Štajner, Tijana, Srbljanović, Jelena, Lijeskić, Olivera, Đurković-Đaković, Olgica, "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange" in Microorganisms, 9, no. 12 (2021):2526,
https://doi.org/10.3390/microorganisms9122526 . .

TY  - JOUR
AU  - Lijeskić, Olivera
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Radosavljević, Aleksandra
AU  - Bobić, Branko
AU  - Klun, Ivana
AU  - Stanojević-Paović, Anka
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1198
AB  - Introduction: Ocular toxoplasmosis is the most common cause of infectious posterior uveitis worldwide. It can be prenatal or postnatal in origin. Despite estimations that postnatal ocular toxoplasmosis is more prevalent, only several cases of proven postnatal ocular toxoplasmosis have been reported in non-epidemic settings. Here, the clinical evolution of ocular toxoplasmosis of conclusively proven postnatal origin in immunocompetent patients is reported.Methodology: Postnatal ocular toxoplasmosis was diagnosed based on clinical diagnosis supported by the longitudinal detection of Toxoplasma gondii-specific IgG, IgM and IgA antibodies in the serum as well as by direct detection of the parasite (bioassay) and/or its DNA (real-time PCR) in aqueous humor.Results: Three cases involved adults in whom ocular toxoplasmosis developed during primary T. gondii infection, as part of the clinical presentation in two and as the sole manifestation in one patient. The fourth patient was a case of inactive ocular toxoplasmosis in a 14-year-old boy, where postnatal infection was confirmed by exclusion of maternal infection. The causative parasite strain was genotyped in only one case and it belonged to genotype II, the dominant type in Europe. One patient acquired the infection in Africa, suggesting an atypical strain.Conclusions: The distinction between prenatal and postnatal ocular toxoplasmosis is only possible in particular clinical situations, and requires extensive laboratory investigation. Genotyping of the parasite strain involved may be important, particularly if atypical strains are suspected, requiring tailored treatment approaches.
T2  - The Journal of Infection in Developing Countries
T1  - Postnatal ocular toxoplasmosis in immunocompetent patients
EP  - 1522
IS  - 10
SP  - 1515
VL  - 15
DO  - 10.3855/jidc.14824
ER  - 

@article{
author = "Lijeskić, Olivera and Štajner, Tijana and Srbljanović, Jelena and Radosavljević, Aleksandra and Bobić, Branko and Klun, Ivana and Stanojević-Paović, Anka and Đurković-Đaković, Olgica",
year = "2021",
abstract = "Introduction: Ocular toxoplasmosis is the most common cause of infectious posterior uveitis worldwide. It can be prenatal or postnatal in origin. Despite estimations that postnatal ocular toxoplasmosis is more prevalent, only several cases of proven postnatal ocular toxoplasmosis have been reported in non-epidemic settings. Here, the clinical evolution of ocular toxoplasmosis of conclusively proven postnatal origin in immunocompetent patients is reported.Methodology: Postnatal ocular toxoplasmosis was diagnosed based on clinical diagnosis supported by the longitudinal detection of Toxoplasma gondii-specific IgG, IgM and IgA antibodies in the serum as well as by direct detection of the parasite (bioassay) and/or its DNA (real-time PCR) in aqueous humor.Results: Three cases involved adults in whom ocular toxoplasmosis developed during primary T. gondii infection, as part of the clinical presentation in two and as the sole manifestation in one patient. The fourth patient was a case of inactive ocular toxoplasmosis in a 14-year-old boy, where postnatal infection was confirmed by exclusion of maternal infection. The causative parasite strain was genotyped in only one case and it belonged to genotype II, the dominant type in Europe. One patient acquired the infection in Africa, suggesting an atypical strain.Conclusions: The distinction between prenatal and postnatal ocular toxoplasmosis is only possible in particular clinical situations, and requires extensive laboratory investigation. Genotyping of the parasite strain involved may be important, particularly if atypical strains are suspected, requiring tailored treatment approaches.",
journal = "The Journal of Infection in Developing Countries",
title = "Postnatal ocular toxoplasmosis in immunocompetent patients",
pages = "1522-1515",
number = "10",
volume = "15",
doi = "10.3855/jidc.14824"
}

Lijeskić, O., Štajner, T., Srbljanović, J., Radosavljević, A., Bobić, B., Klun, I., Stanojević-Paović, A.,& Đurković-Đaković, O.. (2021). Postnatal ocular toxoplasmosis in immunocompetent patients. in The Journal of Infection in Developing Countries, 15(10), 1515-1522.
https://doi.org/10.3855/jidc.14824

Lijeskić O, Štajner T, Srbljanović J, Radosavljević A, Bobić B, Klun I, Stanojević-Paović A, Đurković-Đaković O. Postnatal ocular toxoplasmosis in immunocompetent patients. in The Journal of Infection in Developing Countries. 2021;15(10):1515-1522.
doi:10.3855/jidc.14824 .

Lijeskić, Olivera, Štajner, Tijana, Srbljanović, Jelena, Radosavljević, Aleksandra, Bobić, Branko, Klun, Ivana, Stanojević-Paović, Anka, Đurković-Đaković, Olgica, "Postnatal ocular toxoplasmosis in immunocompetent patients" in The Journal of Infection in Developing Countries, 15, no. 10 (2021):1515-1522,
https://doi.org/10.3855/jidc.14824 . .

TY  - JOUR
AU  - Bobić, Branko
AU  - Ćirković, Vladimir
AU  - Klun, Ivana
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1267
AB  - Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.
PB  - Cambridge University Press
T2  - Journal of Helminthology
T1  - Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review
SP  - e49
VL  - 95
DO  - 10.1017/S0022149X21000432
ER  - 

@article{
author = "Bobić, Branko and Ćirković, Vladimir and Klun, Ivana and Štajner, Tijana and Srbljanović, Jelena and Bauman, Neda and Đurković-Đaković, Olgica",
year = "2021",
abstract = "Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.",
publisher = "Cambridge University Press",
journal = "Journal of Helminthology",
title = "Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review",
pages = "e49",
volume = "95",
doi = "10.1017/S0022149X21000432"
}

Bobić, B., Ćirković, V., Klun, I., Štajner, T., Srbljanović, J., Bauman, N.,& Đurković-Đaković, O.. (2021). Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review. in Journal of Helminthology
Cambridge University Press., 95, e49.
https://doi.org/10.1017/S0022149X21000432

Bobić B, Ćirković V, Klun I, Štajner T, Srbljanović J, Bauman N, Đurković-Đaković O. Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review. in Journal of Helminthology. 2021;95:e49.
doi:10.1017/S0022149X21000432 .

Bobić, Branko, Ćirković, Vladimir, Klun, Ivana, Štajner, Tijana, Srbljanović, Jelena, Bauman, Neda, Đurković-Đaković, Olgica, "Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review" in Journal of Helminthology, 95 (2021):e49,
https://doi.org/10.1017/S0022149X21000432 . .

TY  - JOUR
AU  - Deng, Huifang
AU  - Cummins, Rachel
AU  - Schares, Gereon
AU  - Trevisan, Chiara
AU  - Enemark, Heidi
AU  - Waap, Helga
AU  - Srbljanović, Jelena
AU  - Đurković-Đaković, Olgica
AU  - Pires, Sara Monteiro
AU  - van der Giessen, Joke
AU  - Opsteegh, Marieke
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1080
AB  - Background: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect virtually all warm-blooded animals. It is the causative agent of toxoplasmosis, a significant public health issue worldwide. Mathematical models are useful to study the transmission dynamics of T. gondii infection in different settings, and may be used to compare the effectiveness of prevention measures. Methods: To obtain an overview of existing mathematical models for transmission of T. gondii, a systematic review was undertaken. The review was conducted according to an a priori protocol and the results were reported according to the PRISMA guidelines. Specific search terms were developed and used in the search of three databases (Scopus, PubMed, and Embase). Results: In total, 484 unique records were retrieved from the systematic search. Among them, 15 studies that used mathematical models to study the transmission of T. gondii. These studies were categorized into four groups based on the primary aims: dynamics of transmission (n = 8), intervention (n = 5), spatial distribution (n = 1), and outbreak investigation (n = 1). Conclusions: Considering the high disease burden caused by T. gondii, the number of studies using mathematical models to understand the transmission dynamics of this parasite and to evaluate the effectiveness of intervention measures was only 15. This systematic review provides an overview of existing mathematical models and identifies the data gaps for model building. The results from this study can be helpful for further development of mathematical models and improved understanding of the transmission dynamics of T. gondii infection.
T2  - Food & Waterborne Parasitology
T1  - Mathematical modelling of Toxoplasma gondii transmission: A systematic review
SP  - e00102
VL  - 22
DO  - 10.1016/j.fawpar.2020.e00102
ER  - 

@article{
author = "Deng, Huifang and Cummins, Rachel and Schares, Gereon and Trevisan, Chiara and Enemark, Heidi and Waap, Helga and Srbljanović, Jelena and Đurković-Đaković, Olgica and Pires, Sara Monteiro and van der Giessen, Joke and Opsteegh, Marieke",
year = "2021",
abstract = "Background: Toxoplasma gondii is a ubiquitous protozoan parasite that can infect virtually all warm-blooded animals. It is the causative agent of toxoplasmosis, a significant public health issue worldwide. Mathematical models are useful to study the transmission dynamics of T. gondii infection in different settings, and may be used to compare the effectiveness of prevention measures. Methods: To obtain an overview of existing mathematical models for transmission of T. gondii, a systematic review was undertaken. The review was conducted according to an a priori protocol and the results were reported according to the PRISMA guidelines. Specific search terms were developed and used in the search of three databases (Scopus, PubMed, and Embase). Results: In total, 484 unique records were retrieved from the systematic search. Among them, 15 studies that used mathematical models to study the transmission of T. gondii. These studies were categorized into four groups based on the primary aims: dynamics of transmission (n = 8), intervention (n = 5), spatial distribution (n = 1), and outbreak investigation (n = 1). Conclusions: Considering the high disease burden caused by T. gondii, the number of studies using mathematical models to understand the transmission dynamics of this parasite and to evaluate the effectiveness of intervention measures was only 15. This systematic review provides an overview of existing mathematical models and identifies the data gaps for model building. The results from this study can be helpful for further development of mathematical models and improved understanding of the transmission dynamics of T. gondii infection.",
journal = "Food & Waterborne Parasitology",
title = "Mathematical modelling of Toxoplasma gondii transmission: A systematic review",
pages = "e00102",
volume = "22",
doi = "10.1016/j.fawpar.2020.e00102"
}

Deng, H., Cummins, R., Schares, G., Trevisan, C., Enemark, H., Waap, H., Srbljanović, J., Đurković-Đaković, O., Pires, S. M., van der Giessen, J.,& Opsteegh, M.. (2021). Mathematical modelling of Toxoplasma gondii transmission: A systematic review. in Food & Waterborne Parasitology, 22, e00102.
https://doi.org/10.1016/j.fawpar.2020.e00102

Deng H, Cummins R, Schares G, Trevisan C, Enemark H, Waap H, Srbljanović J, Đurković-Đaković O, Pires SM, van der Giessen J, Opsteegh M. Mathematical modelling of Toxoplasma gondii transmission: A systematic review. in Food & Waterborne Parasitology. 2021;22:e00102.
doi:10.1016/j.fawpar.2020.e00102 .

Deng, Huifang, Cummins, Rachel, Schares, Gereon, Trevisan, Chiara, Enemark, Heidi, Waap, Helga, Srbljanović, Jelena, Đurković-Đaković, Olgica, Pires, Sara Monteiro, van der Giessen, Joke, Opsteegh, Marieke, "Mathematical modelling of Toxoplasma gondii transmission: A systematic review" in Food & Waterborne Parasitology, 22 (2021):e00102,
https://doi.org/10.1016/j.fawpar.2020.e00102 . .

TY  - JOUR
AU  - Nedisan, Maria E.
AU  - Gyorke, Adriana
AU  - Stefanut, Cristina L.
AU  - Kalmar, Zsuzsa
AU  - Friss, Zsuzsa
AU  - Blaga, Radu
AU  - Blaizot, Amandine
AU  - Toma-Naić, Andra
AU  - Mircean, Viorica
AU  - Schares, Gereon
AU  - Đurković-Đaković, Olgica
AU  - Klun, Ivana
AU  - Villena, Isabelle
AU  - Cozma, Vasile
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1078
AB  - Toxoplasma gondii is a widespread zoonotic protozoan that infects most species of mammals and birds, including poultry. This study aimed to investigate the course of T. gondii infection and the efficacy of diclazuril and Artemisia annua in preventing infection in experimentally infected chickens. Seventy-five 1-month-old chickens, female and male, were randomly divided into five groups (n = 15 each) as follows: (1) uninfected untreated (negative control, NC); (2) infected with T. gondii genotype II/III isolated from a wild cat (group WC); (3) infected with T. gondii genotype II isolated from a domestic cat (group DC); (4) infected with T. gondii domestic cat strain and treated with the anticoccidial diclazuril (group DC-D); and (5) infected with T. gondii domestic cat strain and treated with the medicinal plant Artemisia annua (group DC-A). Clinical signs, body temperature, mortality rate, weight gain, feed conversion ratio, hematological parameters, and the presence of T. gondii-specific IgY antibodies were recorded in all groups. Five chickens per group were euthanized 28 days post-infection (p.i.) and their brains, hearts, and breast muscle tested for T. gondii by mouse bioassay and polymerase chain reaction (PCR). No clinical signs related to the experimental infection were observed throughout the study period. T. gondii-specific antibodies were detected by day 28 p.i., but not in all infected chickens. Overall, T. gondii DNA was detected (bioassay or tissue digests) in all infected and untreated chickens (10/10), while viable parasite (bioassay) was isolated from 7 out of 10 chickens. The parasite was most frequently identified in the brain (7/10). There were no differences in the T. gondii strains regarding clinical infection and the rate of T. gondii detection in tissues. However, higher antibody titers were obtained in chickens infected with T. gondii WC strain (1:192) comparing with T. gondii DC strain (1:48). A. annua reduced replication of the parasite in 3 out of 5 chickens, while diclazuril did not. In conclusion, broiler chickens were resistant to clinical toxoplasmosis, irrespective of the strain (domestic or wild cat strain). The herb A. annua presented prophylactic efficacy by reduced parasite replication. However, further studies are required aiming at the efficacy of diclazuril and A. annua for the prevention of T. gondii infection in chickens using quantitative analysis methods.
PB  - Springer, New York
T2  - Parasitology Research
T1  - Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis
EP  - 603
IS  - 2
SP  - 593
VL  - 120
DO  - 10.1007/s00436-020-06984-x
ER  - 

@article{
author = "Nedisan, Maria E. and Gyorke, Adriana and Stefanut, Cristina L. and Kalmar, Zsuzsa and Friss, Zsuzsa and Blaga, Radu and Blaizot, Amandine and Toma-Naić, Andra and Mircean, Viorica and Schares, Gereon and Đurković-Đaković, Olgica and Klun, Ivana and Villena, Isabelle and Cozma, Vasile",
year = "2021",
abstract = "Toxoplasma gondii is a widespread zoonotic protozoan that infects most species of mammals and birds, including poultry. This study aimed to investigate the course of T. gondii infection and the efficacy of diclazuril and Artemisia annua in preventing infection in experimentally infected chickens. Seventy-five 1-month-old chickens, female and male, were randomly divided into five groups (n = 15 each) as follows: (1) uninfected untreated (negative control, NC); (2) infected with T. gondii genotype II/III isolated from a wild cat (group WC); (3) infected with T. gondii genotype II isolated from a domestic cat (group DC); (4) infected with T. gondii domestic cat strain and treated with the anticoccidial diclazuril (group DC-D); and (5) infected with T. gondii domestic cat strain and treated with the medicinal plant Artemisia annua (group DC-A). Clinical signs, body temperature, mortality rate, weight gain, feed conversion ratio, hematological parameters, and the presence of T. gondii-specific IgY antibodies were recorded in all groups. Five chickens per group were euthanized 28 days post-infection (p.i.) and their brains, hearts, and breast muscle tested for T. gondii by mouse bioassay and polymerase chain reaction (PCR). No clinical signs related to the experimental infection were observed throughout the study period. T. gondii-specific antibodies were detected by day 28 p.i., but not in all infected chickens. Overall, T. gondii DNA was detected (bioassay or tissue digests) in all infected and untreated chickens (10/10), while viable parasite (bioassay) was isolated from 7 out of 10 chickens. The parasite was most frequently identified in the brain (7/10). There were no differences in the T. gondii strains regarding clinical infection and the rate of T. gondii detection in tissues. However, higher antibody titers were obtained in chickens infected with T. gondii WC strain (1:192) comparing with T. gondii DC strain (1:48). A. annua reduced replication of the parasite in 3 out of 5 chickens, while diclazuril did not. In conclusion, broiler chickens were resistant to clinical toxoplasmosis, irrespective of the strain (domestic or wild cat strain). The herb A. annua presented prophylactic efficacy by reduced parasite replication. However, further studies are required aiming at the efficacy of diclazuril and A. annua for the prevention of T. gondii infection in chickens using quantitative analysis methods.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis",
pages = "603-593",
number = "2",
volume = "120",
doi = "10.1007/s00436-020-06984-x"
}

Nedisan, M. E., Gyorke, A., Stefanut, C. L., Kalmar, Z., Friss, Z., Blaga, R., Blaizot, A., Toma-Naić, A., Mircean, V., Schares, G., Đurković-Đaković, O., Klun, I., Villena, I.,& Cozma, V.. (2021). Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis. in Parasitology Research
Springer, New York., 120(2), 593-603.
https://doi.org/10.1007/s00436-020-06984-x

Nedisan ME, Gyorke A, Stefanut CL, Kalmar Z, Friss Z, Blaga R, Blaizot A, Toma-Naić A, Mircean V, Schares G, Đurković-Đaković O, Klun I, Villena I, Cozma V. Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis. in Parasitology Research. 2021;120(2):593-603.
doi:10.1007/s00436-020-06984-x .

Nedisan, Maria E., Gyorke, Adriana, Stefanut, Cristina L., Kalmar, Zsuzsa, Friss, Zsuzsa, Blaga, Radu, Blaizot, Amandine, Toma-Naić, Andra, Mircean, Viorica, Schares, Gereon, Đurković-Đaković, Olgica, Klun, Ivana, Villena, Isabelle, Cozma, Vasile, "Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis" in Parasitology Research, 120, no. 2 (2021):593-603,
https://doi.org/10.1007/s00436-020-06984-x . .

TY  - JOUR
AU  - van der Giessen, Joke
AU  - Deksne, Gunita
AU  - Gómez-Morales, Maria Angeles
AU  - Troell, Karin
AU  - Gomes, Jacinto
AU  - Sotiraki, Smaragda
AU  - Rozycki, Miroslaw
AU  - Kucsera, Istvan
AU  - Đurković-Đaković, Olgica
AU  - Robertson, Lucy J.
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1088
AB  - In 2012, WHO/FAO ranked 24 foodborne parasites (FBP) using multicriteria decision analysis (MCDA) to provide risk assessors with a basis for prioritising control of highly ranked FBP on the global level. One conclusion was that ranking may differ substantially per region. In Europe, the same methodology was used to rank FBP of relevance for Europe. Of the 24 FBP, the top-five prioritised FBP were identified for Europe as Echinococcus multilocularis, Toxoplasma gondii, Trichinella spiralis, E. granulosus, and Cryptosporidium spp., all of which are zoonotic. The objective of the present study was to provide an overview of surveillance and reporting systems in Europe for these top five prioritised FBP in the human and animal populations, to identify gaps, and give recommendations for improvement. Information on the surveillance systems was collected from 35 European countries and analysed according to the five different regions. For most FBP, human surveillance is passive in most countries and regions in Europe and notification differs between countries and regions. Adequate surveillance programmes for these FBP are lacking, except for T. spiralis, which is notifiable in 34 countries with active surveillance in susceptible animals under EU directive. Although human and animal surveillance data are available for the five prioritised FBP, we identified a lack of consistency in surveillance and reporting requirements between national experts and European bodies. Recommendations for improved surveillance systems are discussed.
T2  - Parasite Epidemiology & Control
T1  - Surveillance of foodborne parasitic diseases in Europe in a One Health approach
VL  - 13
DO  - 10.1016/j.parepi.2021.e00205
ER  - 

@article{
author = "van der Giessen, Joke and Deksne, Gunita and Gómez-Morales, Maria Angeles and Troell, Karin and Gomes, Jacinto and Sotiraki, Smaragda and Rozycki, Miroslaw and Kucsera, Istvan and Đurković-Đaković, Olgica and Robertson, Lucy J.",
year = "2021",
abstract = "In 2012, WHO/FAO ranked 24 foodborne parasites (FBP) using multicriteria decision analysis (MCDA) to provide risk assessors with a basis for prioritising control of highly ranked FBP on the global level. One conclusion was that ranking may differ substantially per region. In Europe, the same methodology was used to rank FBP of relevance for Europe. Of the 24 FBP, the top-five prioritised FBP were identified for Europe as Echinococcus multilocularis, Toxoplasma gondii, Trichinella spiralis, E. granulosus, and Cryptosporidium spp., all of which are zoonotic. The objective of the present study was to provide an overview of surveillance and reporting systems in Europe for these top five prioritised FBP in the human and animal populations, to identify gaps, and give recommendations for improvement. Information on the surveillance systems was collected from 35 European countries and analysed according to the five different regions. For most FBP, human surveillance is passive in most countries and regions in Europe and notification differs between countries and regions. Adequate surveillance programmes for these FBP are lacking, except for T. spiralis, which is notifiable in 34 countries with active surveillance in susceptible animals under EU directive. Although human and animal surveillance data are available for the five prioritised FBP, we identified a lack of consistency in surveillance and reporting requirements between national experts and European bodies. Recommendations for improved surveillance systems are discussed.",
journal = "Parasite Epidemiology & Control",
title = "Surveillance of foodborne parasitic diseases in Europe in a One Health approach",
volume = "13",
doi = "10.1016/j.parepi.2021.e00205"
}

van der Giessen, J., Deksne, G., Gómez-Morales, M. A., Troell, K., Gomes, J., Sotiraki, S., Rozycki, M., Kucsera, I., Đurković-Đaković, O.,& Robertson, L. J.. (2021). Surveillance of foodborne parasitic diseases in Europe in a One Health approach. in Parasite Epidemiology & Control, 13.
https://doi.org/10.1016/j.parepi.2021.e00205

van der Giessen J, Deksne G, Gómez-Morales MA, Troell K, Gomes J, Sotiraki S, Rozycki M, Kucsera I, Đurković-Đaković O, Robertson LJ. Surveillance of foodborne parasitic diseases in Europe in a One Health approach. in Parasite Epidemiology & Control. 2021;13.
doi:10.1016/j.parepi.2021.e00205 .

van der Giessen, Joke, Deksne, Gunita, Gómez-Morales, Maria Angeles, Troell, Karin, Gomes, Jacinto, Sotiraki, Smaragda, Rozycki, Miroslaw, Kucsera, Istvan, Đurković-Đaković, Olgica, Robertson, Lucy J., "Surveillance of foodborne parasitic diseases in Europe in a One Health approach" in Parasite Epidemiology & Control, 13 (2021),
https://doi.org/10.1016/j.parepi.2021.e00205 . .

TY  - JOUR
AU  - Srbljanović, Jelena
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Uzelac, Aleksandra
AU  - Opsenica, Igor
AU  - Terzić-Jovanović, Nataša
AU  - Bauman, Neda
AU  - Šolaja, Bogdan
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/998
AB  - Objectives: Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. Methods: In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 and CQ-resistant (CQ(R)) P. falciparum strain Dd2. In vivo activity was evaluated by a modified Thompson test using C57BL/6 mice infected with Plasmodium berghei ANKA strain. Results: Nine of the ten compounds had a lower 50% inhibitory concentration (IC50) than CQ against the CQ(R) strain Dd2. Five of these compounds that were available for in vivo evaluation were shown to be nontoxic. All five compounds administered at a dose of 160 mg/kg/day for 3 days prolonged the survival of treated compared with untreated mice. Untreated control mice died by Day 7 with a mean parasitaemia of 15%. Among treated mice, a dichotomous outcome was observed, with a two-third majority of treated mice dying by Day 17 with a low mean parasitaemia of 5%, whilst one-third survived longer with a mean hyperparasitaemia of 70%; specifically, five of these mice survived a mean of 25 days, whilst two even survived past Day 31. Conclusions: The significant antimalarial potential of this aminoquinoline series is illustrated by its excellent in vitro activity against the CQ(R) P. falciparum strain and significant in vivo activity. Interestingly, compounds CIAQ7, CIAQ9 and CIAQ11 were able to confer resistance to cerebral malaria and afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Global Antimicrobial Resistance
T1  - Aminoquinolines afford resistance to cerebral malaria in susceptible mice
EP  - 25
SP  - 20
VL  - 23
DO  - 10.1016/j.jgar.2020.07.027
ER  - 

@article{
author = "Srbljanović, Jelena and Bobić, Branko and Štajner, Tijana and Uzelac, Aleksandra and Opsenica, Igor and Terzić-Jovanović, Nataša and Bauman, Neda and Šolaja, Bogdan and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Objectives: Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. Methods: In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 and CQ-resistant (CQ(R)) P. falciparum strain Dd2. In vivo activity was evaluated by a modified Thompson test using C57BL/6 mice infected with Plasmodium berghei ANKA strain. Results: Nine of the ten compounds had a lower 50% inhibitory concentration (IC50) than CQ against the CQ(R) strain Dd2. Five of these compounds that were available for in vivo evaluation were shown to be nontoxic. All five compounds administered at a dose of 160 mg/kg/day for 3 days prolonged the survival of treated compared with untreated mice. Untreated control mice died by Day 7 with a mean parasitaemia of 15%. Among treated mice, a dichotomous outcome was observed, with a two-third majority of treated mice dying by Day 17 with a low mean parasitaemia of 5%, whilst one-third survived longer with a mean hyperparasitaemia of 70%; specifically, five of these mice survived a mean of 25 days, whilst two even survived past Day 31. Conclusions: The significant antimalarial potential of this aminoquinoline series is illustrated by its excellent in vitro activity against the CQ(R) P. falciparum strain and significant in vivo activity. Interestingly, compounds CIAQ7, CIAQ9 and CIAQ11 were able to confer resistance to cerebral malaria and afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Global Antimicrobial Resistance",
title = "Aminoquinolines afford resistance to cerebral malaria in susceptible mice",
pages = "25-20",
volume = "23",
doi = "10.1016/j.jgar.2020.07.027"
}

Srbljanović, J., Bobić, B., Štajner, T., Uzelac, A., Opsenica, I., Terzić-Jovanović, N., Bauman, N., Šolaja, B.,& Đurković-Đaković, O.. (2020). Aminoquinolines afford resistance to cerebral malaria in susceptible mice. in Journal of Global Antimicrobial Resistance
Elsevier Sci Ltd, Oxford., 23, 20-25.
https://doi.org/10.1016/j.jgar.2020.07.027

Srbljanović J, Bobić B, Štajner T, Uzelac A, Opsenica I, Terzić-Jovanović N, Bauman N, Šolaja B, Đurković-Đaković O. Aminoquinolines afford resistance to cerebral malaria in susceptible mice. in Journal of Global Antimicrobial Resistance. 2020;23:20-25.
doi:10.1016/j.jgar.2020.07.027 .

Srbljanović, Jelena, Bobić, Branko, Štajner, Tijana, Uzelac, Aleksandra, Opsenica, Igor, Terzić-Jovanović, Nataša, Bauman, Neda, Šolaja, Bogdan, Đurković-Đaković, Olgica, "Aminoquinolines afford resistance to cerebral malaria in susceptible mice" in Journal of Global Antimicrobial Resistance, 23 (2020):20-25,
https://doi.org/10.1016/j.jgar.2020.07.027 . .

TY  - JOUR
AU  - Bauman, Neda
AU  - Ilić, Anđelija
AU  - Lijeskić, Olivera
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Srbljanović, Jelena
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1046
AB  - Toxoplasma gondiiis an obligate intracellular parasite infecting up to one third of the human population. The central event in the pathogenesis of toxoplasmosis is the conversion of tachyzoites into encysted bradyzoites. A novel approach to analyze the structure ofin vivo-derived tissue cysts may be the increasingly used computational image analysis. The objective of this study was to quantify the geometrical complexity ofT.gondiicysts by morphological, particle, and fractal analysis, as well as to determine if it is impacted by parasite strain, cyst age, and host type. A total of 31 images ofT.gondiibrain cysts of four type-2 strains (Me49, and local isolates BGD1, BGD14, and BGD26) was analyzed using ImageJ software. The parameters of interest included diameter, circularity, packing density (PD), fractal dimension (FD), and lacunarity. Although cyst diameter varied widely, its negative correlation with PD was observed. Circularity was remarkably close to 1, indicating a perfectly round shape of the cysts. PD and FD did not vary among cysts of different strains, age, and derived from mice of different genetic background. Conversely, lacunarity, which is a measure of heterogeneity, was significantly lower for BGD1 strain vs. all other strains, and higher for Me49 vs. BGD14 and BGD26, but did not differ among Me49 cysts of different age, or those derived from genetically different mice. The results indicate a highly uniform structure and occupancy of the differentT.gondiitissue cysts. This study furthers the use of image analysis in describing the structural complexity ofT.gondiicyst morphology, and presents the first application of fractal analysis for this purpose. The presented results show that use of a freely available software is a cost-effective approach to advance automated image scoring forT.gondiicysts.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts
IS  - 8
SP  - e0234169
VL  - 15
DO  - 10.1371/journal.pone.0234169
ER  - 

@article{
author = "Bauman, Neda and Ilić, Anđelija and Lijeskić, Olivera and Uzelac, Aleksandra and Klun, Ivana and Srbljanović, Jelena and Ćirković, Vladimir and Bobić, Branko and Štajner, Tijana and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Toxoplasma gondiiis an obligate intracellular parasite infecting up to one third of the human population. The central event in the pathogenesis of toxoplasmosis is the conversion of tachyzoites into encysted bradyzoites. A novel approach to analyze the structure ofin vivo-derived tissue cysts may be the increasingly used computational image analysis. The objective of this study was to quantify the geometrical complexity ofT.gondiicysts by morphological, particle, and fractal analysis, as well as to determine if it is impacted by parasite strain, cyst age, and host type. A total of 31 images ofT.gondiibrain cysts of four type-2 strains (Me49, and local isolates BGD1, BGD14, and BGD26) was analyzed using ImageJ software. The parameters of interest included diameter, circularity, packing density (PD), fractal dimension (FD), and lacunarity. Although cyst diameter varied widely, its negative correlation with PD was observed. Circularity was remarkably close to 1, indicating a perfectly round shape of the cysts. PD and FD did not vary among cysts of different strains, age, and derived from mice of different genetic background. Conversely, lacunarity, which is a measure of heterogeneity, was significantly lower for BGD1 strain vs. all other strains, and higher for Me49 vs. BGD14 and BGD26, but did not differ among Me49 cysts of different age, or those derived from genetically different mice. The results indicate a highly uniform structure and occupancy of the differentT.gondiitissue cysts. This study furthers the use of image analysis in describing the structural complexity ofT.gondiicyst morphology, and presents the first application of fractal analysis for this purpose. The presented results show that use of a freely available software is a cost-effective approach to advance automated image scoring forT.gondiicysts.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts",
number = "8",
pages = "e0234169",
volume = "15",
doi = "10.1371/journal.pone.0234169"
}

Bauman, N., Ilić, A., Lijeskić, O., Uzelac, A., Klun, I., Srbljanović, J., Ćirković, V., Bobić, B., Štajner, T.,& Đurković-Đaković, O.. (2020). Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts. in PLoS One
Public Library Science, San Francisco., 15(8), e0234169.
https://doi.org/10.1371/journal.pone.0234169

Bauman N, Ilić A, Lijeskić O, Uzelac A, Klun I, Srbljanović J, Ćirković V, Bobić B, Štajner T, Đurković-Đaković O. Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts. in PLoS One. 2020;15(8):e0234169.
doi:10.1371/journal.pone.0234169 .

Bauman, Neda, Ilić, Anđelija, Lijeskić, Olivera, Uzelac, Aleksandra, Klun, Ivana, Srbljanović, Jelena, Ćirković, Vladimir, Bobić, Branko, Štajner, Tijana, Đurković-Đaković, Olgica, "Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts" in PLoS One, 15, no. 8 (2020):e0234169,
https://doi.org/10.1371/journal.pone.0234169 . .

TY  - JOUR
AU  - Ćirković, Vladimir
AU  - Klun, Ivana
AU  - Utaaker, Kjersti Selstad
AU  - Uzelac, Aleksandra
AU  - Tysnes, Kristoffer R.
AU  - Robertson, Lucy J.
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/999
AB  - Giardiasis and cryptosporidiosis are recognized by the WHO as important emerging diseases of the 21st century. Symptoms are similar and include diarrhoea and vomiting, which may be severe, even life-threatening, for the immunocompromised and children under five years of age. Between 2013 and 2017, the Institute for Public Health in Serbia recorded 10 waterborne epidemics that manifested as gastrointestinal disease. Routine testing for enteropathogenic bacteria and viruses did not identify the aetiological agents of these outbreaks. As water is not examined for the presence of protozoa in Serbia, we performed a pilot study to analyse samples from four major rivers and their tributaries using a newly implemented methodology for detection of Giardia and Cryptosporidium, based on the ISO 15553:2006 standard. Using immunofluorescence microscopy, Giardia was detected in 10 out of the 31 samples, Cryptosporidium in five, while two samples were positive for both. Presence of G. duodenalis gDNA was confirmed by amplification of the beta-giardin gene in eight samples, of which one and two, respectively, were identified by RFLP as potentially zoonotic assemblages A and B. The results suggest that surface water in Serbia may be a potential source of infection and call for more in-depth studies using sophisticated molecular tools.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental Parasitology
T1  - Surface waters as a potential source of Giardia and Cryptosporidium in Serbia
SP  - 107824
VL  - 209
DO  - 10.1016/j.exppara.2019.107824
ER  - 

@article{
author = "Ćirković, Vladimir and Klun, Ivana and Utaaker, Kjersti Selstad and Uzelac, Aleksandra and Tysnes, Kristoffer R. and Robertson, Lucy J. and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Giardiasis and cryptosporidiosis are recognized by the WHO as important emerging diseases of the 21st century. Symptoms are similar and include diarrhoea and vomiting, which may be severe, even life-threatening, for the immunocompromised and children under five years of age. Between 2013 and 2017, the Institute for Public Health in Serbia recorded 10 waterborne epidemics that manifested as gastrointestinal disease. Routine testing for enteropathogenic bacteria and viruses did not identify the aetiological agents of these outbreaks. As water is not examined for the presence of protozoa in Serbia, we performed a pilot study to analyse samples from four major rivers and their tributaries using a newly implemented methodology for detection of Giardia and Cryptosporidium, based on the ISO 15553:2006 standard. Using immunofluorescence microscopy, Giardia was detected in 10 out of the 31 samples, Cryptosporidium in five, while two samples were positive for both. Presence of G. duodenalis gDNA was confirmed by amplification of the beta-giardin gene in eight samples, of which one and two, respectively, were identified by RFLP as potentially zoonotic assemblages A and B. The results suggest that surface water in Serbia may be a potential source of infection and call for more in-depth studies using sophisticated molecular tools.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental Parasitology",
title = "Surface waters as a potential source of Giardia and Cryptosporidium in Serbia",
pages = "107824",
volume = "209",
doi = "10.1016/j.exppara.2019.107824"
}

Ćirković, V., Klun, I., Utaaker, K. S., Uzelac, A., Tysnes, K. R., Robertson, L. J.,& Đurković-Đaković, O.. (2020). Surface waters as a potential source of Giardia and Cryptosporidium in Serbia. in Experimental Parasitology
Academic Press Inc Elsevier Science, San Diego., 209, 107824.
https://doi.org/10.1016/j.exppara.2019.107824

Ćirković V, Klun I, Utaaker KS, Uzelac A, Tysnes KR, Robertson LJ, Đurković-Đaković O. Surface waters as a potential source of Giardia and Cryptosporidium in Serbia. in Experimental Parasitology. 2020;209:107824.
doi:10.1016/j.exppara.2019.107824 .

Ćirković, Vladimir, Klun, Ivana, Utaaker, Kjersti Selstad, Uzelac, Aleksandra, Tysnes, Kristoffer R., Robertson, Lucy J., Đurković-Đaković, Olgica, "Surface waters as a potential source of Giardia and Cryptosporidium in Serbia" in Experimental Parasitology, 209 (2020):107824,
https://doi.org/10.1016/j.exppara.2019.107824 . .

TY  - JOUR
AU  - Ćirković, Vladimir
AU  - Uzelac, Aleksandra
AU  - Miličić, Dragana
AU  - Klun, Ivana
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1009
AB  - Toxoplasma gondii is one of the most successful parasites in the world. Its life form found in the environment, where it may stay viable for a year or more, is the oocyst, excreted by members of the family Felidae as the definite host. During the past decades, several waterborne epidemics of toxoplasmosis worldwide indicated the importance of water as a source of T. gondii infection. In Serbia, detection of oocysts in water has not been previously performed and, therefore, there are no data regarding the presence of T. gondii in Serbian rivers. To fill this gap, surface water samples (n=20) were collected from four major rivers and their tributaries throughout Serbia. The samples were concentrated by filtration and total DNA was extracted from the pellet using a commercial kit. Next, the samples were screened for T. gondii DNA by amplification of the 529 bp repeat fragment by conventional PCR. Three positive samples were detected and all three were confirmed by amplification of GRA6 and SAG2 genes. This first detection of T. gondii in river waters in Serbia suggests that the examined rivers are contaminated with this protozoan, which makes them a potential source of infection. In addition, this study has shown that the used PCR methodology can successfully detect T. gondii in water samples.
PB  - Inst Zoology, Bas, Sofia
T2  - Acta Zoologica Bulgarica
T1  - First Detection of Toxoplasma gondii (Nicolle & Manceaux, 1908) (Eucoccidiorida: Sarcocystidae) in River Waters in Serbia
EP  - 83
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1009
ER  - 

@article{
author = "Ćirković, Vladimir and Uzelac, Aleksandra and Miličić, Dragana and Klun, Ivana and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Toxoplasma gondii is one of the most successful parasites in the world. Its life form found in the environment, where it may stay viable for a year or more, is the oocyst, excreted by members of the family Felidae as the definite host. During the past decades, several waterborne epidemics of toxoplasmosis worldwide indicated the importance of water as a source of T. gondii infection. In Serbia, detection of oocysts in water has not been previously performed and, therefore, there are no data regarding the presence of T. gondii in Serbian rivers. To fill this gap, surface water samples (n=20) were collected from four major rivers and their tributaries throughout Serbia. The samples were concentrated by filtration and total DNA was extracted from the pellet using a commercial kit. Next, the samples were screened for T. gondii DNA by amplification of the 529 bp repeat fragment by conventional PCR. Three positive samples were detected and all three were confirmed by amplification of GRA6 and SAG2 genes. This first detection of T. gondii in river waters in Serbia suggests that the examined rivers are contaminated with this protozoan, which makes them a potential source of infection. In addition, this study has shown that the used PCR methodology can successfully detect T. gondii in water samples.",
publisher = "Inst Zoology, Bas, Sofia",
journal = "Acta Zoologica Bulgarica",
title = "First Detection of Toxoplasma gondii (Nicolle & Manceaux, 1908) (Eucoccidiorida: Sarcocystidae) in River Waters in Serbia",
pages = "83-79",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1009"
}

Ćirković, V., Uzelac, A., Miličić, D., Klun, I.,& Đurković-Đaković, O.. (2020). First Detection of Toxoplasma gondii (Nicolle & Manceaux, 1908) (Eucoccidiorida: Sarcocystidae) in River Waters in Serbia. in Acta Zoologica Bulgarica
Inst Zoology, Bas, Sofia., 79-83.
https://hdl.handle.net/21.15107/rcub_rimi_1009

Ćirković V, Uzelac A, Miličić D, Klun I, Đurković-Đaković O. First Detection of Toxoplasma gondii (Nicolle & Manceaux, 1908) (Eucoccidiorida: Sarcocystidae) in River Waters in Serbia. in Acta Zoologica Bulgarica. 2020;:79-83.
https://hdl.handle.net/21.15107/rcub_rimi_1009 .

Ćirković, Vladimir, Uzelac, Aleksandra, Miličić, Dragana, Klun, Ivana, Đurković-Đaković, Olgica, "First Detection of Toxoplasma gondii (Nicolle & Manceaux, 1908) (Eucoccidiorida: Sarcocystidae) in River Waters in Serbia" in Acta Zoologica Bulgarica (2020):79-83,
https://hdl.handle.net/21.15107/rcub_rimi_1009 .

TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Ćirković, Vladimir
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1015
AB  - Toxoplasma gondii archetypes II and III are mildly virulent, yet virulence of variant strains is largely unknown. While lineage II dominates in humans in Europe, lineage III strains are present in various intermediate hosts. In Serbia, lineage III represents 24% of the population structure and occurs most frequently in domestic animals, implying a significant presence in the human food web. In this study, the virulence of four genetically distinct lineage III variants was assessed in vivo and in vitro. In vivo, two strains were shown to be intermediately virulent and two mildly virulent, with cumulative mortalities of 69.4%, 38.8%, 10.7%, and 6.8%, respectively. The strain with the highest mortality has previously been isolated in Europe and may be endemic; the strain with the lowest mortality matches ToxoDB#54, while the remaining two represent novel genotypes. Identical alleles were detected at ROP5, ROP16, ROP18, and GRA15. A set of in vitro analyses revealed proliferation and plaque formation as virulence factors. Higher levels of expression of ENO2 in intermediately virulent strains point to enhanced metabolism as the underlying mechanism. The results suggest that metabolic attenuation, and possibly stage conversion, may be delayed in virulent strains.
PB  - MDPI, Basel
T2  - Microorganisms
T1  - In Vivo and In Vitro Virulence Analysis of Four Genetically Distinct Toxoplasma gondii Lineage III Isolates
IS  - 11
VL  - 8
DO  - 10.3390/microorganisms8111702
ER  - 

@article{
author = "Uzelac, Aleksandra and Klun, Ivana and Ćirković, Vladimir and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Toxoplasma gondii archetypes II and III are mildly virulent, yet virulence of variant strains is largely unknown. While lineage II dominates in humans in Europe, lineage III strains are present in various intermediate hosts. In Serbia, lineage III represents 24% of the population structure and occurs most frequently in domestic animals, implying a significant presence in the human food web. In this study, the virulence of four genetically distinct lineage III variants was assessed in vivo and in vitro. In vivo, two strains were shown to be intermediately virulent and two mildly virulent, with cumulative mortalities of 69.4%, 38.8%, 10.7%, and 6.8%, respectively. The strain with the highest mortality has previously been isolated in Europe and may be endemic; the strain with the lowest mortality matches ToxoDB#54, while the remaining two represent novel genotypes. Identical alleles were detected at ROP5, ROP16, ROP18, and GRA15. A set of in vitro analyses revealed proliferation and plaque formation as virulence factors. Higher levels of expression of ENO2 in intermediately virulent strains point to enhanced metabolism as the underlying mechanism. The results suggest that metabolic attenuation, and possibly stage conversion, may be delayed in virulent strains.",
publisher = "MDPI, Basel",
journal = "Microorganisms",
title = "In Vivo and In Vitro Virulence Analysis of Four Genetically Distinct Toxoplasma gondii Lineage III Isolates",
number = "11",
volume = "8",
doi = "10.3390/microorganisms8111702"
}

Uzelac, A., Klun, I., Ćirković, V.,& Đurković-Đaković, O.. (2020). In Vivo and In Vitro Virulence Analysis of Four Genetically Distinct Toxoplasma gondii Lineage III Isolates. in Microorganisms
MDPI, Basel., 8(11).
https://doi.org/10.3390/microorganisms8111702

Uzelac A, Klun I, Ćirković V, Đurković-Đaković O. In Vivo and In Vitro Virulence Analysis of Four Genetically Distinct Toxoplasma gondii Lineage III Isolates. in Microorganisms. 2020;8(11).
doi:10.3390/microorganisms8111702 .

Uzelac, Aleksandra, Klun, Ivana, Ćirković, Vladimir, Đurković-Đaković, Olgica, "In Vivo and In Vitro Virulence Analysis of Four Genetically Distinct Toxoplasma gondii Lineage III Isolates" in Microorganisms, 8, no. 11 (2020),
https://doi.org/10.3390/microorganisms8111702 . .

TY  - JOUR
AU  - Kuruca, Ljiljana
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Lalošević, Vesna
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/954
AB  - Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Toxoplasma gondii genotypes circulating in domestic pigs in Serbia
EP  - 211
IS  - 2
SP  - 204
VL  - 67
DO  - 10.1556/004.2019.022
ER  - 

@article{
author = "Kuruca, Ljiljana and Uzelac, Aleksandra and Klun, Ivana and Lalošević, Vesna and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Toxoplasma gondii genotypes circulating in domestic pigs in Serbia",
pages = "211-204",
number = "2",
volume = "67",
doi = "10.1556/004.2019.022"
}

Kuruca, L., Uzelac, A., Klun, I., Lalošević, V.,& Đurković-Đaković, O.. (2019). Toxoplasma gondii genotypes circulating in domestic pigs in Serbia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(2), 204-211.
https://doi.org/10.1556/004.2019.022

Kuruca L, Uzelac A, Klun I, Lalošević V, Đurković-Đaković O. Toxoplasma gondii genotypes circulating in domestic pigs in Serbia. in Acta Veterinaria Hungarica. 2019;67(2):204-211.
doi:10.1556/004.2019.022 .

Kuruca, Ljiljana, Uzelac, Aleksandra, Klun, Ivana, Lalošević, Vesna, Đurković-Đaković, Olgica, "Toxoplasma gondii genotypes circulating in domestic pigs in Serbia" in Acta Veterinaria Hungarica, 67, no. 2 (2019):204-211,
https://doi.org/10.1556/004.2019.022 . .

TY  - JOUR
AU  - Stopić, Milena
AU  - Bobić, Branko
AU  - Dakić, Zorica
AU  - Srbljanović, Jelena
AU  - Štajner, Tijana
AU  - Konstantinović, Neda M.
AU  - Srećković, Katarina
AU  - Klun, Ivana
AU  - Korac, Miloš
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/909
AB  - Objectives: As is the case for all of Southeast Europe, Serbia is an area traditionally endemic for Taenia saginata and Taenia solium infections. This study was performed to analyse the epidemiological data on taeniosis and cysticercosis in Serbia for the period 1990-2018. Methods: Data on cases of T. saginata and T. solium infection were collected via a systematic search of published articles, the grey literature, and official reports, as well as by performing clinical observational studies of patients treated in the departments for infectious diseases of hospitals and university clinics in Serbia. Results: A total of 212 cases of taeniosis were reported, all between 1997 and 2004 when taeniosis was notifiable (incidence range 0.04-0.9/100 000 population/year). From 1990 to 2018, 170 cases of cysticercosis (all but one of neurocysticercosis), were registered (incidence range 0-0.29/100 000 population/year), with a strong decrease since 2000 and a single case in the last 9 years. The annual number of cases of both taeniosis (Pearson's r = 0.914, p = 0.001) and cysticercosis (Pearson's r = 0.582, p = 0.014) correlated with the consumer price index. Conclusions: In Serbia, T. saginata and T. solium infections are autochthonous but occur only sporadically. However, the potential for re-emergence exists, depending on the socio-economic state of the country.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Journal of Infectious Diseases
T1  - Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living
EP  - 141
SP  - 135
VL  - 86
DO  - 10.1016/j.ijid.2019.07.010
ER  - 

@article{
author = "Stopić, Milena and Bobić, Branko and Dakić, Zorica and Srbljanović, Jelena and Štajner, Tijana and Konstantinović, Neda M. and Srećković, Katarina and Klun, Ivana and Korac, Miloš and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Objectives: As is the case for all of Southeast Europe, Serbia is an area traditionally endemic for Taenia saginata and Taenia solium infections. This study was performed to analyse the epidemiological data on taeniosis and cysticercosis in Serbia for the period 1990-2018. Methods: Data on cases of T. saginata and T. solium infection were collected via a systematic search of published articles, the grey literature, and official reports, as well as by performing clinical observational studies of patients treated in the departments for infectious diseases of hospitals and university clinics in Serbia. Results: A total of 212 cases of taeniosis were reported, all between 1997 and 2004 when taeniosis was notifiable (incidence range 0.04-0.9/100 000 population/year). From 1990 to 2018, 170 cases of cysticercosis (all but one of neurocysticercosis), were registered (incidence range 0-0.29/100 000 population/year), with a strong decrease since 2000 and a single case in the last 9 years. The annual number of cases of both taeniosis (Pearson's r = 0.914, p = 0.001) and cysticercosis (Pearson's r = 0.582, p = 0.014) correlated with the consumer price index. Conclusions: In Serbia, T. saginata and T. solium infections are autochthonous but occur only sporadically. However, the potential for re-emergence exists, depending on the socio-economic state of the country.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal of Infectious Diseases",
title = "Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living",
pages = "141-135",
volume = "86",
doi = "10.1016/j.ijid.2019.07.010"
}

Stopić, M., Bobić, B., Dakić, Z., Srbljanović, J., Štajner, T., Konstantinović, N. M., Srećković, K., Klun, I., Korac, M.,& Đurković-Đaković, O.. (2019). Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living. in International Journal of Infectious Diseases
Elsevier Sci Ltd, Oxford., 86, 135-141.
https://doi.org/10.1016/j.ijid.2019.07.010

Stopić M, Bobić B, Dakić Z, Srbljanović J, Štajner T, Konstantinović NM, Srećković K, Klun I, Korac M, Đurković-Đaković O. Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living. in International Journal of Infectious Diseases. 2019;86:135-141.
doi:10.1016/j.ijid.2019.07.010 .

Stopić, Milena, Bobić, Branko, Dakić, Zorica, Srbljanović, Jelena, Štajner, Tijana, Konstantinović, Neda M., Srećković, Katarina, Klun, Ivana, Korac, Miloš, Đurković-Đaković, Olgica, "Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living" in International Journal of Infectious Diseases, 86 (2019):135-141,
https://doi.org/10.1016/j.ijid.2019.07.010 . .

TY  - JOUR
AU  - Trevisan, Chiara
AU  - Sotiraki, Smaragda
AU  - Laranjo-Gonzalez, Minerva
AU  - Dermauw, Veronique
AU  - Wang, Ziqi
AU  - Kaerssin, Age
AU  - Cvetkovikj, Aleksandar
AU  - Winkler, Andrea S.
AU  - Abraham, Annette
AU  - Bobić, Branko
AU  - Lassen, Brian
AU  - Cretu, Carmen Michaela
AU  - Vasile, Cozma
AU  - Arvanitis, Dimitris
AU  - Deksne, Gunita
AU  - Ilievski, Boro
AU  - Kucsera, Istvan
AU  - Karamon, Jacek
AU  - Stefanovska, Jovana
AU  - Koudela, Bretislav
AU  - Jurhar-Pavlova, Maja
AU  - Varady, Marian
AU  - Pavlak, Marina
AU  - Sarkunas, Mindaugas
AU  - Kaminski, Miriam
AU  - Đurković-Đaković, Olgica
AU  - Jokelainen, Pikka
AU  - Jan, Dagny Stojcevic
AU  - Schmidt, Veronika
AU  - Dakić, Zorica
AU  - Gabriel, Sarah
AU  - Dorny, Pierre
AU  - Devleesschauwer, Brecht
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/963
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018)
VL  - 12
DO  - 10.1186/s13071-019-3328-8
ER  - 

@article{
author = "Trevisan, Chiara and Sotiraki, Smaragda and Laranjo-Gonzalez, Minerva and Dermauw, Veronique and Wang, Ziqi and Kaerssin, Age and Cvetkovikj, Aleksandar and Winkler, Andrea S. and Abraham, Annette and Bobić, Branko and Lassen, Brian and Cretu, Carmen Michaela and Vasile, Cozma and Arvanitis, Dimitris and Deksne, Gunita and Ilievski, Boro and Kucsera, Istvan and Karamon, Jacek and Stefanovska, Jovana and Koudela, Bretislav and Jurhar-Pavlova, Maja and Varady, Marian and Pavlak, Marina and Sarkunas, Mindaugas and Kaminski, Miriam and Đurković-Đaković, Olgica and Jokelainen, Pikka and Jan, Dagny Stojcevic and Schmidt, Veronika and Dakić, Zorica and Gabriel, Sarah and Dorny, Pierre and Devleesschauwer, Brecht",
year = "2019",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018)",
volume = "12",
doi = "10.1186/s13071-019-3328-8"
}

Trevisan, C., Sotiraki, S., Laranjo-Gonzalez, M., Dermauw, V., Wang, Z., Kaerssin, A., Cvetkovikj, A., Winkler, A. S., Abraham, A., Bobić, B., Lassen, B., Cretu, C. M., Vasile, C., Arvanitis, D., Deksne, G., Ilievski, B., Kucsera, I., Karamon, J., Stefanovska, J., Koudela, B., Jurhar-Pavlova, M., Varady, M., Pavlak, M., Sarkunas, M., Kaminski, M., Đurković-Đaković, O., Jokelainen, P., Jan, D. S., Schmidt, V., Dakić, Z., Gabriel, S., Dorny, P.,& Devleesschauwer, B.. (2019). Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018). in Parasites & Vectors
BMC, London., 12.
https://doi.org/10.1186/s13071-019-3328-8

Trevisan C, Sotiraki S, Laranjo-Gonzalez M, Dermauw V, Wang Z, Kaerssin A, Cvetkovikj A, Winkler AS, Abraham A, Bobić B, Lassen B, Cretu CM, Vasile C, Arvanitis D, Deksne G, Ilievski B, Kucsera I, Karamon J, Stefanovska J, Koudela B, Jurhar-Pavlova M, Varady M, Pavlak M, Sarkunas M, Kaminski M, Đurković-Đaković O, Jokelainen P, Jan DS, Schmidt V, Dakić Z, Gabriel S, Dorny P, Devleesschauwer B. Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018). in Parasites & Vectors. 2019;12.
doi:10.1186/s13071-019-3328-8 .

Trevisan, Chiara, Sotiraki, Smaragda, Laranjo-Gonzalez, Minerva, Dermauw, Veronique, Wang, Ziqi, Kaerssin, Age, Cvetkovikj, Aleksandar, Winkler, Andrea S., Abraham, Annette, Bobić, Branko, Lassen, Brian, Cretu, Carmen Michaela, Vasile, Cozma, Arvanitis, Dimitris, Deksne, Gunita, Ilievski, Boro, Kucsera, Istvan, Karamon, Jacek, Stefanovska, Jovana, Koudela, Bretislav, Jurhar-Pavlova, Maja, Varady, Marian, Pavlak, Marina, Sarkunas, Mindaugas, Kaminski, Miriam, Đurković-Đaković, Olgica, Jokelainen, Pikka, Jan, Dagny Stojcevic, Schmidt, Veronika, Dakić, Zorica, Gabriel, Sarah, Dorny, Pierre, Devleesschauwer, Brecht, "Epidemiology of taeniosis/cysticercosis in Europe, a systematic review: eastern Europe (vol 11, 569, 2018)" in Parasites & Vectors, 12 (2019),
https://doi.org/10.1186/s13071-019-3328-8 . .

TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Ćirković, Vladimir
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/906
AB  - To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains.
PB  - Elsevier Ireland Ltd, Clare
T2  - Parasitology International
T1  - Detection and genotyping of Toxoplasma gondii in wild canids in Serbia
VL  - 73
DO  - 10.1016/j.parint.2019.101973
ER  - 

@article{
author = "Uzelac, Aleksandra and Klun, Ivana and Ćirović, Duško and Penezić, Aleksandra and Ćirković, Vladimir and Đurković-Đaković, Olgica",
year = "2019",
abstract = "To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Parasitology International",
title = "Detection and genotyping of Toxoplasma gondii in wild canids in Serbia",
volume = "73",
doi = "10.1016/j.parint.2019.101973"
}

Uzelac, A., Klun, I., Ćirović, D., Penezić, A., Ćirković, V.,& Đurković-Đaković, O.. (2019). Detection and genotyping of Toxoplasma gondii in wild canids in Serbia. in Parasitology International
Elsevier Ireland Ltd, Clare., 73.
https://doi.org/10.1016/j.parint.2019.101973

Uzelac A, Klun I, Ćirović D, Penezić A, Ćirković V, Đurković-Đaković O. Detection and genotyping of Toxoplasma gondii in wild canids in Serbia. in Parasitology International. 2019;73.
doi:10.1016/j.parint.2019.101973 .

Uzelac, Aleksandra, Klun, Ivana, Ćirović, Duško, Penezić, Aleksandra, Ćirković, Vladimir, Đurković-Đaković, Olgica, "Detection and genotyping of Toxoplasma gondii in wild canids in Serbia" in Parasitology International, 73 (2019),
https://doi.org/10.1016/j.parint.2019.101973 . .