TY  - JOUR
AU  - Kuruca, Ljiljana
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Lalošević, Vesna
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/954
AB  - Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Toxoplasma gondii genotypes circulating in domestic pigs in Serbia
EP  - 211
IS  - 2
SP  - 204
VL  - 67
DO  - 10.1556/004.2019.022
ER  - 

@article{
author = "Kuruca, Ljiljana and Uzelac, Aleksandra and Klun, Ivana and Lalošević, Vesna and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Consumption of undercooked or raw pork is considered a significant risk factor for human infection with Toxoplasma gondii. In this study, we investigated the genetic structure of 18 T. gondii strains obtained from slaughter pigs from Northern Serbia (mainly Vojvodina). The examined samples originated from eight pigs from large commercial farms, six backyard pigs and four free-range Mangalica pigs, all found to be positive for either viable T. gondii or T. gondii DNA. Genotyping was attempted from both pig tissues and mouse brains from the bioassays using a multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP) method with seven markers (GRA6,.lt. SAG2, PK-1, BTUB, C22-8, CS3 and Apico). Identification was achieved for nine T. gondii isolates. Seven isolates were classified as type II and two as type III. These results are consistent with previous studies on animal isolates from Serbia as well as with previous reports that type III is more frequently found in samples from Southern Europe than in those from other parts of the continent.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Toxoplasma gondii genotypes circulating in domestic pigs in Serbia",
pages = "211-204",
number = "2",
volume = "67",
doi = "10.1556/004.2019.022"
}

Kuruca, L., Uzelac, A., Klun, I., Lalošević, V.,& Đurković-Đaković, O.. (2019). Toxoplasma gondii genotypes circulating in domestic pigs in Serbia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(2), 204-211.
https://doi.org/10.1556/004.2019.022

Kuruca L, Uzelac A, Klun I, Lalošević V, Đurković-Đaković O. Toxoplasma gondii genotypes circulating in domestic pigs in Serbia. in Acta Veterinaria Hungarica. 2019;67(2):204-211.
doi:10.1556/004.2019.022 .

Kuruca, Ljiljana, Uzelac, Aleksandra, Klun, Ivana, Lalošević, Vesna, Đurković-Đaković, Olgica, "Toxoplasma gondii genotypes circulating in domestic pigs in Serbia" in Acta Veterinaria Hungarica, 67, no. 2 (2019):204-211,
https://doi.org/10.1556/004.2019.022 . .

TY  - JOUR
AU  - Klun, Ivana
AU  - Ćosić, Nada
AU  - Ćirović, Duško
AU  - Vasilev, Dragan
AU  - Teodorović, Vlado
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/941
AB  - Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6.2%, 95% CI = 4.0-8.4) animals, including 14 red foxes (4.7%), 7 wild cats (35%), 5 beech martens (4.8%), 2 pine martens (16.7%), and 1 European badger (6.25%). No Trichinella larvae were detected in the examined specimens of European polecats, steppe polecats and European otters. Species identification of the Trichinella larvae performed for 18 positive samples revealed T. spiralis in 77.8% and T. britovi in 22.2% of the isolates. Both species were detected in red foxes and wild cats. The predominance of T. spiralis in wildlife in Serbia indicates the (past or present) spillover of this pathogen from domestic to wild animals.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Trichinella spp. In wild mesocarnivores in an endemic setting
EP  - 39
IS  - 1
SP  - 34
VL  - 67
DO  - 10.1556/004.2019.004
ER  - 

@article{
author = "Klun, Ivana and Ćosić, Nada and Ćirović, Duško and Vasilev, Dragan and Teodorović, Vlado and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Human trichinellosis and Trichinella infection in pigs are both still endemic in the Balkans, including Serbia. Because of the flow between the sylvatic and the domestic cycle of Trichinella spp., monitoring wildlife has been recommended for the risk assessment of Trichinella spp. infection in swine. We have previously shown the presence of Trichinella infection in wild carnivores including the wolf and the golden jackal, and here we report on Trichinella infection in several other mesocarnivore species. From a total of 469 animals collected between 1994 and 2013, Trichinella larvae were detected in 29 (6.2%, 95% CI = 4.0-8.4) animals, including 14 red foxes (4.7%), 7 wild cats (35%), 5 beech martens (4.8%), 2 pine martens (16.7%), and 1 European badger (6.25%). No Trichinella larvae were detected in the examined specimens of European polecats, steppe polecats and European otters. Species identification of the Trichinella larvae performed for 18 positive samples revealed T. spiralis in 77.8% and T. britovi in 22.2% of the isolates. Both species were detected in red foxes and wild cats. The predominance of T. spiralis in wildlife in Serbia indicates the (past or present) spillover of this pathogen from domestic to wild animals.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Trichinella spp. In wild mesocarnivores in an endemic setting",
pages = "39-34",
number = "1",
volume = "67",
doi = "10.1556/004.2019.004"
}

Klun, I., Ćosić, N., Ćirović, D., Vasilev, D., Teodorović, V.,& Đurković-Đaković, O.. (2019). Trichinella spp. In wild mesocarnivores in an endemic setting. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(1), 34-39.
https://doi.org/10.1556/004.2019.004

Klun I, Ćosić N, Ćirović D, Vasilev D, Teodorović V, Đurković-Đaković O. Trichinella spp. In wild mesocarnivores in an endemic setting. in Acta Veterinaria Hungarica. 2019;67(1):34-39.
doi:10.1556/004.2019.004 .

Klun, Ivana, Ćosić, Nada, Ćirović, Duško, Vasilev, Dragan, Teodorović, Vlado, Đurković-Đaković, Olgica, "Trichinella spp. In wild mesocarnivores in an endemic setting" in Acta Veterinaria Hungarica, 67, no. 1 (2019):34-39,
https://doi.org/10.1556/004.2019.004 . .

TY  - JOUR
AU  - Kuruca, Ljiljana
AU  - Klun, Ivana
AU  - Uzelac, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Bobić, Branko
AU  - Simin, Stanislav
AU  - Lalošević, Vesna
AU  - Lalošević, Dušan
AU  - Đurković-Đaković, Olgica
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/793
AB  - Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.
PB  - Springer, New York
T2  - Parasitology Research
T1  - Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia
EP  - 3123
IS  - 11
SP  - 3117
VL  - 116
DO  - 10.1007/s00436-017-5623-7
ER  - 

@article{
author = "Kuruca, Ljiljana and Klun, Ivana and Uzelac, Aleksandra and Nikolić, Aleksandra and Bobić, Branko and Simin, Stanislav and Lalošević, Vesna and Lalošević, Dušan and Đurković-Đaković, Olgica",
year = "2017",
abstract = "Insufficiently cooked pork is considered as an important source of human infection with Toxoplasma gondii. The aim of our study was to investigate the presence of T. gondii in pigs intended for human consumption from Northern Serbia. Blood and diaphragm samples were collected from 182 naturally infected market-weight pigs, originating from both commercial farms and smallholdings. Sera were examined using modified agglutination test (MAT), and diaphragms from seropositive, as well as from some MAT-negative pigs, were bioassayed in mice. In addition, digests were examined for the presence of T. gondii DNA using a real-time polymerase chain reaction (qPCR) which was targeted at the 529 bp repetitive element of the T. gondii genome. The overall seroprevalence of T. gondii in pigs was 17% (31/182), with no difference between pigs from large commercial farms (17.8%) and those raised on smallholdings (16.3%). However, the seroprevalence in farm pigs was largely influenced by the findings on a single farm, where all examined animals tested positive. Parasites and/or parasite DNA were detected in the tissues of 15 of the 45 (25 seropositive and 20 seronegative) animals examined by either direct method. Tissue cysts were isolated in eight bioassays and an additional bioassay was positive by serology; all nine were confirmed positive by qPCR. All positive bioassays originated from seropositive pigs, but no correlation was observed between isolation rate and antibody titer. T. gondii DNA was detected in diaphragm tissues of eight pigs, of which three were seronegative. The results of our study provide further evidence for pork as a source of human T. gondii infection.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia",
pages = "3123-3117",
number = "11",
volume = "116",
doi = "10.1007/s00436-017-5623-7"
}

Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Lalošević, V., Lalošević, D.,& Đurković-Đaković, O.. (2017). Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. in Parasitology Research
Springer, New York., 116(11), 3117-3123.
https://doi.org/10.1007/s00436-017-5623-7

Kuruca L, Klun I, Uzelac A, Nikolić A, Bobić B, Simin S, Lalošević V, Lalošević D, Đurković-Đaković O. Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia. in Parasitology Research. 2017;116(11):3117-3123.
doi:10.1007/s00436-017-5623-7 .

Kuruca, Ljiljana, Klun, Ivana, Uzelac, Aleksandra, Nikolić, Aleksandra, Bobić, Branko, Simin, Stanislav, Lalošević, Vesna, Lalošević, Dušan, Đurković-Đaković, Olgica, "Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia" in Parasitology Research, 116, no. 11 (2017):3117-3123,
https://doi.org/10.1007/s00436-017-5623-7 . .

TY  - JOUR
AU  - Kuruca, Ljiljana
AU  - Klun, Ivana
AU  - Uzelac, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Bobić, Branko
AU  - Simin, Stanislav
AU  - Đurković-Đaković, Olgica
AU  - Lalošević, Vesna
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/713
AB  - Toxoplasma gondii is considered one of the most successful parasites of humans and animals. The ingestion of viable cysts through the consumption of undercooked pork is recognized as a significant route of human infection with T. gondii. The aim of this study was to investigate the presence of viable parasite in tissues of free-range pigs from the Zasavica Special Nature Reserve. All pigs were of the Mangulica breed, raised in a traditional way. The serological screening was performed using a modified agglutination test (MAT). The isolation of viable T. gondii was attempted by a bioassay of pig heart tissue in mice, while the real-time polymerase chain reaction (qPCR) targeted at the 529 bp repetitive element of T. gondii was used to detect parasitic DNA in digested hearts. Specific antibodies were detected in 12 out of 18 pigs examined. The bioassay was performed for five MAT-positive and one MAT-negative pig, and a total of three isolates were obtained. qPCR was performed for all samples, including one MAT-negative sample that was not bioassayed. The presence of T. gondii DNA was confirmed in all hearts with a positive bioassay as well as in one originating from seropositive and one from seronegative pig whose hearts were not bioassayed. The successful isolation of viable cysts, presence of risk factors (such as older age at the time of slaughter) and increased contact with the environment, along with the great appreciation of Serbian consumers towards home-cured Mangulica’s meat, make this breed worthy of consideration as a potentially important reservoir of human infection.
AB  - Toxoplasma gondii predstavlja jednog od najuspešnijih parazita ljudi i životinja. Ingestija vijabilnih cista, putem konzumacije termički nedovoljno obrađenog mesa, smatra se jednim od dominantnih puteva humane infekcije ovim parazitom. Cilj ovog istraživanja bio je da se ispita prisustvo vijabilnih parazita u tkivima slobodno držanih svinja u Specijalnom rezervatu prirode “Zasavica”. Sve ispitane svinje su pripadale rasi mangulica i gajene su na tradicionalan način. Serološka ispitivanja su sprovedena upotrebom testa modifikovane aglutinacije (MAT). Za izolaciju vijabilnih parazita iz tkiva (srca) svinja korišćen je biološki ogled na miševima, dok je reakcija lančane polimeraze u stvarnom vremenu (qPCR), kojom se detektuje 529 bp repetitivni element genoma T. gondii upotrebljena za detekciju parazitske DNK. Specifična antitela su pronađena kod 12 od 18 ispitanih svinja. Biološki ogled je postavljen za 5 MAT-pozitivnih i jednu MAT-negativnu svinju, iz čega su dobijena ukupno tri izolata T. gondii. qPCR je urađen za sve uzorke, uključujući i jedan MAT-negativan uzorak, za koji nije postavljen biološki ogled. DNK T. gondii je dokazana u digestima srca svih svinja iz kojih je parazit uspešno izolovan, kao i kod jedne seropozitivne i jedne seronegativne svinje za čija srca nije postavljen biološki ogled. Izolacija vijabilnih cista, prisustvo faktora rizika kao što je kasnostasnost rase, povećan kontakt sa spoljašnjom sredinom kao i činjenica da su domaći suhomesnati proizvodi od mesa mangulice visoko cenjeni od strane srpskih potrošača, čine ovu rasu svinja potencijalno značajnim rezervoarom T. gondii za ljude.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica
T1  - Detekcija vijabilnog parazita Toxoplasma gondii kod slobodno držanih svinja iz Specijalnog rezervata prirode Zasavica
EP  - 6
IS  - 3-4
SP  - 1
VL  - 65
DO  - 10.1515/contagri-2016-0010
ER  - 

@article{
author = "Kuruca, Ljiljana and Klun, Ivana and Uzelac, Aleksandra and Nikolić, Aleksandra and Bobić, Branko and Simin, Stanislav and Đurković-Đaković, Olgica and Lalošević, Vesna",
year = "2016",
abstract = "Toxoplasma gondii is considered one of the most successful parasites of humans and animals. The ingestion of viable cysts through the consumption of undercooked pork is recognized as a significant route of human infection with T. gondii. The aim of this study was to investigate the presence of viable parasite in tissues of free-range pigs from the Zasavica Special Nature Reserve. All pigs were of the Mangulica breed, raised in a traditional way. The serological screening was performed using a modified agglutination test (MAT). The isolation of viable T. gondii was attempted by a bioassay of pig heart tissue in mice, while the real-time polymerase chain reaction (qPCR) targeted at the 529 bp repetitive element of T. gondii was used to detect parasitic DNA in digested hearts. Specific antibodies were detected in 12 out of 18 pigs examined. The bioassay was performed for five MAT-positive and one MAT-negative pig, and a total of three isolates were obtained. qPCR was performed for all samples, including one MAT-negative sample that was not bioassayed. The presence of T. gondii DNA was confirmed in all hearts with a positive bioassay as well as in one originating from seropositive and one from seronegative pig whose hearts were not bioassayed. The successful isolation of viable cysts, presence of risk factors (such as older age at the time of slaughter) and increased contact with the environment, along with the great appreciation of Serbian consumers towards home-cured Mangulica’s meat, make this breed worthy of consideration as a potentially important reservoir of human infection., Toxoplasma gondii predstavlja jednog od najuspešnijih parazita ljudi i životinja. Ingestija vijabilnih cista, putem konzumacije termički nedovoljno obrađenog mesa, smatra se jednim od dominantnih puteva humane infekcije ovim parazitom. Cilj ovog istraživanja bio je da se ispita prisustvo vijabilnih parazita u tkivima slobodno držanih svinja u Specijalnom rezervatu prirode “Zasavica”. Sve ispitane svinje su pripadale rasi mangulica i gajene su na tradicionalan način. Serološka ispitivanja su sprovedena upotrebom testa modifikovane aglutinacije (MAT). Za izolaciju vijabilnih parazita iz tkiva (srca) svinja korišćen je biološki ogled na miševima, dok je reakcija lančane polimeraze u stvarnom vremenu (qPCR), kojom se detektuje 529 bp repetitivni element genoma T. gondii upotrebljena za detekciju parazitske DNK. Specifična antitela su pronađena kod 12 od 18 ispitanih svinja. Biološki ogled je postavljen za 5 MAT-pozitivnih i jednu MAT-negativnu svinju, iz čega su dobijena ukupno tri izolata T. gondii. qPCR je urađen za sve uzorke, uključujući i jedan MAT-negativan uzorak, za koji nije postavljen biološki ogled. DNK T. gondii je dokazana u digestima srca svih svinja iz kojih je parazit uspešno izolovan, kao i kod jedne seropozitivne i jedne seronegativne svinje za čija srca nije postavljen biološki ogled. Izolacija vijabilnih cista, prisustvo faktora rizika kao što je kasnostasnost rase, povećan kontakt sa spoljašnjom sredinom kao i činjenica da su domaći suhomesnati proizvodi od mesa mangulice visoko cenjeni od strane srpskih potrošača, čine ovu rasu svinja potencijalno značajnim rezervoarom T. gondii za ljude.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica, Detekcija vijabilnog parazita Toxoplasma gondii kod slobodno držanih svinja iz Specijalnog rezervata prirode Zasavica",
pages = "6-1",
number = "3-4",
volume = "65",
doi = "10.1515/contagri-2016-0010"
}

Kuruca, L., Klun, I., Uzelac, A., Nikolić, A., Bobić, B., Simin, S., Đurković-Đaković, O.,& Lalošević, V.. (2016). Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 65(3-4), 1-6.
https://doi.org/10.1515/contagri-2016-0010

Kuruca L, Klun I, Uzelac A, Nikolić A, Bobić B, Simin S, Đurković-Đaković O, Lalošević V. Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica. in Savremena poljoprivreda. 2016;65(3-4):1-6.
doi:10.1515/contagri-2016-0010 .

Kuruca, Ljiljana, Klun, Ivana, Uzelac, Aleksandra, Nikolić, Aleksandra, Bobić, Branko, Simin, Stanislav, Đurković-Đaković, Olgica, Lalošević, Vesna, "Detection of viable Toxoplasma gondii in free-range pigs from the Special nature reserve of Zasavica" in Savremena poljoprivreda, 65, no. 3-4 (2016):1-6,
https://doi.org/10.1515/contagri-2016-0010 . .

TY  - JOUR
AU  - Ćirović, Duško
AU  - Teodorović, Vlado
AU  - Vasilev, Dragan
AU  - Marković, Marija
AU  - Ćosić, Nada
AU  - Dimitrijević, Mirjana
AU  - Klun, Ivana
AU  - Đurković-Đaković, Olgica
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/623
AB  - Over the last decades the golden jackal (Canis aureus) has significantly expanded its range throughout Southeast and Central Europe, and the Balkan Peninsula is considered to be a core area of the species distribution in this part of the range. Due to its increasing number, ability of long distance movement through a wide range of landscapes and opportunistic feeding habits, the golden jackal may represent an important reservoir and transmitter of a variety of zoonotic agents, including parasites. The Balkans, Serbia included, remain an endemic area for various zoonotic parasites including Trichinella spp. Trichinella has recently been recorded in jackals in Serbia, which prompted us to carry out a large-scale survey of its prevalence, distribution and species identification in this host. In cooperation with local hunters, carcasses of a total of 738 legally hunted golden jackals were collected at 24 localities over an 11-year period (2003-2013). Analysis of tongue base tissue revealed Trichinella larvae in 122, indicating a prevalence of infection of 16.5%. No difference in the prevalence of infection was found between genders 116.2% in males and 16.9% in females (chi(2) = 0.05, p = 0.821)], or among the study years (G = 7.22, p = 0.705). Trichinella larvae were found in 13 out of the 24 examined localities. Molecular identification was performed for 90 isolates, and 64(71.1%) larvae were identified as Trichinella spiralis and 25(27.9%) as Trichinella britovi. Mixed infection (T. spiralis and T. britovi) was recorded in a single case. Although T. spiralis was more prevalent, T. britovi had a wider distribution, and was the only recorded species in jackal populations from the mountainous region of eastern Serbia. On the other hand, T. spiralis was dominant in jackals in the lowlands of central and northern Serbia, where domestic pigs are mostly reared. These results show that the golden jackal is involved in both the domestic and sylvatic cycle, and that it has emerged as a major host species in the sylvatic cycle of the Trichinella genus. Therefore, continued monitoring of Trichinella infection in golden jackals in Serbia and the whole of the Balkans is recommended in order to control transmission of this parasite to humans and domestic animals.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia
EP  - 256
IS  - 3-4
SP  - 253
VL  - 212
DO  - 10.1016/j.vetpar.2015.07.022
ER  - 

@article{
author = "Ćirović, Duško and Teodorović, Vlado and Vasilev, Dragan and Marković, Marija and Ćosić, Nada and Dimitrijević, Mirjana and Klun, Ivana and Đurković-Đaković, Olgica",
year = "2015",
abstract = "Over the last decades the golden jackal (Canis aureus) has significantly expanded its range throughout Southeast and Central Europe, and the Balkan Peninsula is considered to be a core area of the species distribution in this part of the range. Due to its increasing number, ability of long distance movement through a wide range of landscapes and opportunistic feeding habits, the golden jackal may represent an important reservoir and transmitter of a variety of zoonotic agents, including parasites. The Balkans, Serbia included, remain an endemic area for various zoonotic parasites including Trichinella spp. Trichinella has recently been recorded in jackals in Serbia, which prompted us to carry out a large-scale survey of its prevalence, distribution and species identification in this host. In cooperation with local hunters, carcasses of a total of 738 legally hunted golden jackals were collected at 24 localities over an 11-year period (2003-2013). Analysis of tongue base tissue revealed Trichinella larvae in 122, indicating a prevalence of infection of 16.5%. No difference in the prevalence of infection was found between genders 116.2% in males and 16.9% in females (chi(2) = 0.05, p = 0.821)], or among the study years (G = 7.22, p = 0.705). Trichinella larvae were found in 13 out of the 24 examined localities. Molecular identification was performed for 90 isolates, and 64(71.1%) larvae were identified as Trichinella spiralis and 25(27.9%) as Trichinella britovi. Mixed infection (T. spiralis and T. britovi) was recorded in a single case. Although T. spiralis was more prevalent, T. britovi had a wider distribution, and was the only recorded species in jackal populations from the mountainous region of eastern Serbia. On the other hand, T. spiralis was dominant in jackals in the lowlands of central and northern Serbia, where domestic pigs are mostly reared. These results show that the golden jackal is involved in both the domestic and sylvatic cycle, and that it has emerged as a major host species in the sylvatic cycle of the Trichinella genus. Therefore, continued monitoring of Trichinella infection in golden jackals in Serbia and the whole of the Balkans is recommended in order to control transmission of this parasite to humans and domestic animals.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia",
pages = "256-253",
number = "3-4",
volume = "212",
doi = "10.1016/j.vetpar.2015.07.022"
}

Ćirović, D., Teodorović, V., Vasilev, D., Marković, M., Ćosić, N., Dimitrijević, M., Klun, I.,& Đurković-Đaković, O.. (2015). A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 212(3-4), 253-256.
https://doi.org/10.1016/j.vetpar.2015.07.022

Ćirović D, Teodorović V, Vasilev D, Marković M, Ćosić N, Dimitrijević M, Klun I, Đurković-Đaković O. A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia. in Veterinary Parasitology. 2015;212(3-4):253-256.
doi:10.1016/j.vetpar.2015.07.022 .

Ćirović, Duško, Teodorović, Vlado, Vasilev, Dragan, Marković, Marija, Ćosić, Nada, Dimitrijević, Mirjana, Klun, Ivana, Đurković-Đaković, Olgica, "A large-scale study of the Trichinella genus in the golden jackal (Canis aureus) population in Serbia" in Veterinary Parasitology, 212, no. 3-4 (2015):253-256,
https://doi.org/10.1016/j.vetpar.2015.07.022 . .

TY  - JOUR
AU  - Teodorović, Vlado
AU  - Vasilev, Dragan
AU  - Ćirović, Duško
AU  - Marković, Marija
AU  - Ćosić, Nada
AU  - Đurić, Spomenka
AU  - Đurković-Đaković, Olgica
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/537
AB  - Wildlife is the most important reservoir of Trichinella spp. worldwide. Although the Balkans are a recognized European endemic region for Trichinella infections, data on wildlife are scarce. To monitor the circulation of these zoonotic parasites in the Central Balkan region, the wolf (Canis lupus) was selected because of its abundance ( gt 2,000 individuals) and because it is at the top of the food chain. A total of 116 carcasses of wolves were collected in Serbia and in the neighboring areas of Macedonia and Bosnia and Herzegovina (B&H) between 2006 and 2013. Trichinella spp. larvae were found in 54 (46.5%) wolves. The great majority (90.5%) originated from Serbia, where 52 of the 105 examined animals were Trichinella positive (49.5%; 95% confidence interval =39.9-59.1). One positive animal each was found in B&H and Macedonia. All larvae were identified as Trichinella britovi. The high prevalence of Trichinella infection in wolves suggests that this carnivore can be a good indicator species for the risk assessment of the sylvatic Trichinella cycle in the Central Balkans.
PB  - Wildlife Disease Assoc, Inc, Lawrence
T2  - Journal of Wildlife Diseases
T1  - The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans
EP  - 915
IS  - 4
SP  - 911
VL  - 50
DO  - 10.7589/2013-12-333
ER  - 

@article{
author = "Teodorović, Vlado and Vasilev, Dragan and Ćirović, Duško and Marković, Marija and Ćosić, Nada and Đurić, Spomenka and Đurković-Đaković, Olgica",
year = "2014",
abstract = "Wildlife is the most important reservoir of Trichinella spp. worldwide. Although the Balkans are a recognized European endemic region for Trichinella infections, data on wildlife are scarce. To monitor the circulation of these zoonotic parasites in the Central Balkan region, the wolf (Canis lupus) was selected because of its abundance ( gt 2,000 individuals) and because it is at the top of the food chain. A total of 116 carcasses of wolves were collected in Serbia and in the neighboring areas of Macedonia and Bosnia and Herzegovina (B&H) between 2006 and 2013. Trichinella spp. larvae were found in 54 (46.5%) wolves. The great majority (90.5%) originated from Serbia, where 52 of the 105 examined animals were Trichinella positive (49.5%; 95% confidence interval =39.9-59.1). One positive animal each was found in B&H and Macedonia. All larvae were identified as Trichinella britovi. The high prevalence of Trichinella infection in wolves suggests that this carnivore can be a good indicator species for the risk assessment of the sylvatic Trichinella cycle in the Central Balkans.",
publisher = "Wildlife Disease Assoc, Inc, Lawrence",
journal = "Journal of Wildlife Diseases",
title = "The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans",
pages = "915-911",
number = "4",
volume = "50",
doi = "10.7589/2013-12-333"
}

Teodorović, V., Vasilev, D., Ćirović, D., Marković, M., Ćosić, N., Đurić, S.,& Đurković-Đaković, O.. (2014). The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans. in Journal of Wildlife Diseases
Wildlife Disease Assoc, Inc, Lawrence., 50(4), 911-915.
https://doi.org/10.7589/2013-12-333

Teodorović V, Vasilev D, Ćirović D, Marković M, Ćosić N, Đurić S, Đurković-Đaković O. The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans. in Journal of Wildlife Diseases. 2014;50(4):911-915.
doi:10.7589/2013-12-333 .

Teodorović, Vlado, Vasilev, Dragan, Ćirović, Duško, Marković, Marija, Ćosić, Nada, Đurić, Spomenka, Đurković-Đaković, Olgica, "The Wolf (Canis lupus) as an Indicator Species for the Sylvatic Trichinella Cycle in the Central Balkans" in Journal of Wildlife Diseases, 50, no. 4 (2014):911-915,
https://doi.org/10.7589/2013-12-333 . .