TY  - JOUR
AU  - Lijeskić, Olivera
AU  - Bauman, Neda
AU  - Marković, Miloš
AU  - Srbljanović, Jelena
AU  - Bobić, Branko
AU  - Zlatković, Đorđe
AU  - Štajner, Tijana
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1462
AB  - The aim of this study was to evaluate immunogenicity and longevity of the humoral immune response within six months after the homologous (BNT162b2/BNT162b2) or heterologous (BBIBP-CorV/BNT162b2) third dose, and to assess breakthrough infections among vaccinees during the Omicron wave in Serbia. Serum samples were analyzed at four timepoints: five months after the primary series; three weeks, three months, and six months after the boost. IgG antibodies against the receptor-binding domain of the spike protein were detected using enzyme-linked fluorescence assay. Both homologous (n = 55) and heterologous group (n = 36) showed a highly significant increase in antibody concentrations (p < 0.001) three weeks after the boost. A moderate inverse correlation between the age of recipients and the antibody levels at three weeks post-boost was observed in the homologous group (p = 0.02, r = −0.37), while the same correlation was not significant for heterologous group (p = 0.55, r = −0.15). Heterologous group had significantly higher antibody concentrations than homologous group at three weeks (Median 851.4(IQR 766.6–894.1); 784.3(676.9–847.4); p = 0.03) and three months post-boost (766.6(534.8–798.9); 496.8(361.6–664.0); p < 0.001). However, a significant decline in antibody response over time was noted for both strategies. The overall incidence of breakthrough cases was estimated at 36.36% (20/55) for homologous, and 16.67% (6/36) for heterologous group, but none of them required hospitalization. Although observed incidence in the homologous group was more than double when compared to the heterologous group, this difference was not statistically significant, most likely due to the small sample size. In conclusion, waning immunity after inactivated vaccine can be recovered by BNT162b2 heterologous boost regardless of the age of recipients, and both boost strategies induced potent humoral immune response and protection against severe COVID-19 during the Omicron wave. However, as the observed incidence of breakthrough infections was higher in the homologous group, although non-significant, this finding could indicate an advantage of heterologous approach.
PB  - Elsevier
T2  - Vaccine
T2  - Vaccine
T1  - SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost
EP  - 1672
IS  - 7
SP  - 1665
VL  - 42
DO  - 10.1016/j.vaccine.2024.01.085
ER  - 

@article{
author = "Lijeskić, Olivera and Bauman, Neda and Marković, Miloš and Srbljanović, Jelena and Bobić, Branko and Zlatković, Đorđe and Štajner, Tijana",
year = "2024",
abstract = "The aim of this study was to evaluate immunogenicity and longevity of the humoral immune response within six months after the homologous (BNT162b2/BNT162b2) or heterologous (BBIBP-CorV/BNT162b2) third dose, and to assess breakthrough infections among vaccinees during the Omicron wave in Serbia. Serum samples were analyzed at four timepoints: five months after the primary series; three weeks, three months, and six months after the boost. IgG antibodies against the receptor-binding domain of the spike protein were detected using enzyme-linked fluorescence assay. Both homologous (n = 55) and heterologous group (n = 36) showed a highly significant increase in antibody concentrations (p < 0.001) three weeks after the boost. A moderate inverse correlation between the age of recipients and the antibody levels at three weeks post-boost was observed in the homologous group (p = 0.02, r = −0.37), while the same correlation was not significant for heterologous group (p = 0.55, r = −0.15). Heterologous group had significantly higher antibody concentrations than homologous group at three weeks (Median 851.4(IQR 766.6–894.1); 784.3(676.9–847.4); p = 0.03) and three months post-boost (766.6(534.8–798.9); 496.8(361.6–664.0); p < 0.001). However, a significant decline in antibody response over time was noted for both strategies. The overall incidence of breakthrough cases was estimated at 36.36% (20/55) for homologous, and 16.67% (6/36) for heterologous group, but none of them required hospitalization. Although observed incidence in the homologous group was more than double when compared to the heterologous group, this difference was not statistically significant, most likely due to the small sample size. In conclusion, waning immunity after inactivated vaccine can be recovered by BNT162b2 heterologous boost regardless of the age of recipients, and both boost strategies induced potent humoral immune response and protection against severe COVID-19 during the Omicron wave. However, as the observed incidence of breakthrough infections was higher in the homologous group, although non-significant, this finding could indicate an advantage of heterologous approach.",
publisher = "Elsevier",
journal = "Vaccine, Vaccine",
title = "SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost",
pages = "1672-1665",
number = "7",
volume = "42",
doi = "10.1016/j.vaccine.2024.01.085"
}

Lijeskić, O., Bauman, N., Marković, M., Srbljanović, J., Bobić, B., Zlatković, Đ.,& Štajner, T.. (2024). SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost. in Vaccine
Elsevier., 42(7), 1665-1672.
https://doi.org/10.1016/j.vaccine.2024.01.085

Lijeskić O, Bauman N, Marković M, Srbljanović J, Bobić B, Zlatković Đ, Štajner T. SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost. in Vaccine. 2024;42(7):1665-1672.
doi:10.1016/j.vaccine.2024.01.085 .

Lijeskić, Olivera, Bauman, Neda, Marković, Miloš, Srbljanović, Jelena, Bobić, Branko, Zlatković, Đorđe, Štajner, Tijana, "SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: Homologous versus heterologous boost" in Vaccine, 42, no. 7 (2024):1665-1672,
https://doi.org/10.1016/j.vaccine.2024.01.085 . .

TY  - JOUR
AU  - Bauman, Neda
AU  - Srbljanović, Jelena
AU  - Čolović Čalovski, Ivana
AU  - Lijeskić, Olivera
AU  - Ćirković, Vladimir
AU  - Trajković, Jelena
AU  - Bobić, Branko
AU  - Ilić, Andjelija Ž
AU  - Štajner, Tijana
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1473
AB  - Toxoplasma gondii is an obligate intracellular parasite existing in three infectious life stages—tachyzoites, bradyzoites, and sporozoites. Rupture of tissue cysts and re-conversion of bradyzoites to tachyzoites leads to reactivated toxoplasmosis (RT) in an immunocompromised host. The aim of this study was to apply ImageJ software for analysis of T. gondii brain cysts obtained from a newly established in vivo model of RT. Mice chronically infected with T. gondii (BGD1 and BGD26 strains) were treated with cyclophosphamide and hydrocortisone (experimental group—EG) or left untreated as infection controls (ICs). RT in mice was confirmed by qPCR (PCR+); mice remaining chronically infected were PCR−. A total of 90 images of cysts were analyzed for fractal dimension (FD), lacunarity (L), diameter (D), circularity (C), and packing density (PD). Circularity was significantly higher in PCR+ compared to IC mice (p < 0.05 for BGD1, p < 0.001 for the BGD26 strain). A significant negative correlation between D and PD was observed only in IC for the BGD1 strain (ρ = −0.384, p = 0.048), while fractal parameters were stable. Significantly higher D, C, and PD and lower lacunarity, L, were noticed in the BGD1 compared to the more aggressive BGD26 strain. In conclusion, these results demonstrate the complexity of structural alterations of T. gondii cysts in an immunocompromised host and emphasize the application potential of ImageJ in the experimental models of toxoplasmosis.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Fractal and Fractional
T2  - Fractal and Fractional
T1  - Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis
IS  - 3
SP  - 175
VL  - 8
DO  - 10.3390/fractalfract8030175
ER  - 

@article{
author = "Bauman, Neda and Srbljanović, Jelena and Čolović Čalovski, Ivana and Lijeskić, Olivera and Ćirković, Vladimir and Trajković, Jelena and Bobić, Branko and Ilić, Andjelija Ž and Štajner, Tijana",
year = "2024",
abstract = "Toxoplasma gondii is an obligate intracellular parasite existing in three infectious life stages—tachyzoites, bradyzoites, and sporozoites. Rupture of tissue cysts and re-conversion of bradyzoites to tachyzoites leads to reactivated toxoplasmosis (RT) in an immunocompromised host. The aim of this study was to apply ImageJ software for analysis of T. gondii brain cysts obtained from a newly established in vivo model of RT. Mice chronically infected with T. gondii (BGD1 and BGD26 strains) were treated with cyclophosphamide and hydrocortisone (experimental group—EG) or left untreated as infection controls (ICs). RT in mice was confirmed by qPCR (PCR+); mice remaining chronically infected were PCR−. A total of 90 images of cysts were analyzed for fractal dimension (FD), lacunarity (L), diameter (D), circularity (C), and packing density (PD). Circularity was significantly higher in PCR+ compared to IC mice (p < 0.05 for BGD1, p < 0.001 for the BGD26 strain). A significant negative correlation between D and PD was observed only in IC for the BGD1 strain (ρ = −0.384, p = 0.048), while fractal parameters were stable. Significantly higher D, C, and PD and lower lacunarity, L, were noticed in the BGD1 compared to the more aggressive BGD26 strain. In conclusion, these results demonstrate the complexity of structural alterations of T. gondii cysts in an immunocompromised host and emphasize the application potential of ImageJ in the experimental models of toxoplasmosis.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Fractal and Fractional, Fractal and Fractional",
title = "Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis",
number = "3",
pages = "175",
volume = "8",
doi = "10.3390/fractalfract8030175"
}

Bauman, N., Srbljanović, J., Čolović Čalovski, I., Lijeskić, O., Ćirković, V., Trajković, J., Bobić, B., Ilić, A. Ž.,& Štajner, T.. (2024). Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis. in Fractal and Fractional
Multidisciplinary Digital Publishing Institute (MDPI)., 8(3), 175.
https://doi.org/10.3390/fractalfract8030175

Bauman N, Srbljanović J, Čolović Čalovski I, Lijeskić O, Ćirković V, Trajković J, Bobić B, Ilić AŽ, Štajner T. Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis. in Fractal and Fractional. 2024;8(3):175.
doi:10.3390/fractalfract8030175 .

Bauman, Neda, Srbljanović, Jelena, Čolović Čalovski, Ivana, Lijeskić, Olivera, Ćirković, Vladimir, Trajković, Jelena, Bobić, Branko, Ilić, Andjelija Ž, Štajner, Tijana, "Structural Characterization of Toxoplasma gondii Brain Cysts in a Model of Reactivated Toxoplasmosis Using Computational Image Analysis" in Fractal and Fractional, 8, no. 3 (2024):175,
https://doi.org/10.3390/fractalfract8030175 . .

TY  - CONF
AU  - Štajner, Tijana
AU  - Vujić, Dragana
AU  - Nestorović, Emilija
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Lijeskić, Olivera
AU  - Zečević, Željko
AU  - Simić, Marija
AU  - Terzić, Duško
AU  - Jovanović, Snežana
AU  - Dakić, Zorica
AU  - Bobić, Branko
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1450
AB  - Toksoplazmoza je česta ali kod pacijenata lečenih transplantacijom uglavnom zanemarena i pogrešno
dijagnostikovana oportunistička infekcija koja može ugroziti engraftment ali može i evoluirati u
životno ugrožavajuću diseminovanu infekciju. Nakon transplantacije, infekcija parazitom Toxoplasma
gondii se može razviti kao reaktivacija hronične infekcije ili može biti preneta graftom.
Naša osmogodišnja prospektivna studija bila je usmerena na dijagnostiku i monitoring toksoplazmatske
infekcije (TI) kod primalaca matičnih ćelija hematopoeze (haematopoietic stem cell
transplant, HSCT) u centru koji primenjuje protokol uzdržavanja od profilakse do engraftmenta, i
kod primalaca transplantata srca (heart transplant, HT) koji su na kontinuiranoj profilaksi trimetoprim-
sulfametoksazolom (TMP-SMX).
Cilj nam je bio utvrđivanje incidence TI u ova dva vrlo različita transplantaciona režima, i to pre nego
što evoluira u klinički manifestnu, potencijalno fatalnu bolest (Toxoplasma disease, TD). Pre-transplantacioni
serološki i qPCR skrining u post-transplantacionom toku zamenjen je redovnim qPCR
monitoringom iz uzoraka periferne krvi (peripheral blood, PB) usmerenim na Toxoplasma 529 bp gen. Kod primalaca HSCT, qPCR je rađen jednom nedeljno dok je kod primalaca HT qPCR rađen jednom
mesečno prva dva meseca post-HT i potom jednom godišnje. TI je dijagnostikovana na bazi
pozitivnog PCR rezultata iz bar jednog uzorka PB. TI je dijagnostikovana kod 21/104 (20.2%) primalaca
HSCT, prevashodno nakon alogene (19/75) i retko nakon autologne HSCT (2/29). Više od
50% slučajeva TI dijagnostikovano je tokom prvog meseca post-HSCT, pre engraftmenta odnosno
tokom uzdržavanja od profilakse. Sa druge strane, TI je dijagnostikovana kod 3/37 (8.1%) primalaca
HT. Uprkos primeni TMP-SMX, qPCR je postao pozitivan godinu dana posle HT kod dva i dve godine
post-HSCT kod trećeg pacijenta. Infekcija je bila preneta graftom kod 2/3 (seronegativni) a reaktivirana
kod 1/3 primalaca HT (seropozitivni primalac HT poreklom od seropozitivnog donora).
Naši rezultati potvrđuju da je sistemski qPCR monitoring iz uzoraka PB dragocen u dijagnostici TI
ne samo kod primalaca HSCT već i kod primalaca solidnih organa, posebno nakon HT. Učestalost
qPCR monitoringa se mora adaptirati shodno specifičnostima transplantacionog protokola, pre
svega primeni profilakse ali i osnovnoj dijagnozi, na način koji omogućava pravovremenu primenu
specifične terapije u svakom slučaju TI.
AB  - Toxoplasmosis is a common but often neglected and misdiagnosed opportunistic infection in transplant
recipients, which can not only compromise the engraftment, but also evolve into life-threatening
disseminated infection. Post-transplantation, Toxoplasma gondii infection can develop as a reactivation
of chronic infection or could be graft-transmitted. We conducted an eight-year-long prospective
study on the diagnosis and monitoring of Toxoplasma infection (TI) in haematopoietic stem cell
transplant (HSCT) recipients in a setting that withholds prophylaxis until engraftment, and in heart
transplant (HT) recipients on continuous trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis.
The objective was to determine the incidence of TI before it evolves into clinical, potentially fatal
Toxoplasma disease (TD), in these two very different transplantation settings. Pre-transplantation
serological and qPCR screening was followed by post-transplantation peripheral blood (PB)-based
qPCR monitoring targeting the Toxoplasma 529 bp gene. In HSCT recipients, qPCR was performed
weekly while in HT recipients, qPCR was performed monthly for two months post-HT and then
yearly. TI was diagnosed based on a positive PCR result in at least one PB sample.
TI was diagnosed in 21/104 (20.2%) HSCT recipients, predominantly after allogeneic (19/75) and
rarely after autologous HSCT (2/29). Over 50% of TI cases were diagnosed during the first month
post-HSCT, while awaiting engraftment without prophylaxis. On the other hand, TI was diagnosed
in 3/37 (8.1%) HT recipients. Regardless of the TMP-SMX prophylaxis, qPCR became positive one
year after HT in two and two years post-HSCT in third patient. Infection was graft-transmitted in 2/3
(seronegative) and reactivated in 1/3 OHT (seropositive recipient of a seropositive donor’s heart
transplant).
The presented results show that systematic PB-based qPCR monitoring is a valuable resource for
the diagnosis of TI not only in HSCT but also in solid organ recipients, especially after HT. Frequency
of qPCR monitoring should be adjusted according to the specificity of the transplantation setting,
especially in terms of prophylaxis but also an underlying diagnosis, in a manner allowing for prompt
introduction of specific treatment in each case of TI.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije
T1  - Transplantation-related risk of Toxoplasma gondii infection: the National Reference Laboratory prospective cohort study results
EP  - 73
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1450
ER  - 

@conference{
author = "Štajner, Tijana and Vujić, Dragana and Nestorović, Emilija and Srbljanović, Jelena and Bauman, Neda and Lijeskić, Olivera and Zečević, Željko and Simić, Marija and Terzić, Duško and Jovanović, Snežana and Dakić, Zorica and Bobić, Branko",
year = "2023",
abstract = "Toksoplazmoza je česta ali kod pacijenata lečenih transplantacijom uglavnom zanemarena i pogrešno
dijagnostikovana oportunistička infekcija koja može ugroziti engraftment ali može i evoluirati u
životno ugrožavajuću diseminovanu infekciju. Nakon transplantacije, infekcija parazitom Toxoplasma
gondii se može razviti kao reaktivacija hronične infekcije ili može biti preneta graftom.
Naša osmogodišnja prospektivna studija bila je usmerena na dijagnostiku i monitoring toksoplazmatske
infekcije (TI) kod primalaca matičnih ćelija hematopoeze (haematopoietic stem cell
transplant, HSCT) u centru koji primenjuje protokol uzdržavanja od profilakse do engraftmenta, i
kod primalaca transplantata srca (heart transplant, HT) koji su na kontinuiranoj profilaksi trimetoprim-
sulfametoksazolom (TMP-SMX).
Cilj nam je bio utvrđivanje incidence TI u ova dva vrlo različita transplantaciona režima, i to pre nego
što evoluira u klinički manifestnu, potencijalno fatalnu bolest (Toxoplasma disease, TD). Pre-transplantacioni
serološki i qPCR skrining u post-transplantacionom toku zamenjen je redovnim qPCR
monitoringom iz uzoraka periferne krvi (peripheral blood, PB) usmerenim na Toxoplasma 529 bp gen. Kod primalaca HSCT, qPCR je rađen jednom nedeljno dok je kod primalaca HT qPCR rađen jednom
mesečno prva dva meseca post-HT i potom jednom godišnje. TI je dijagnostikovana na bazi
pozitivnog PCR rezultata iz bar jednog uzorka PB. TI je dijagnostikovana kod 21/104 (20.2%) primalaca
HSCT, prevashodno nakon alogene (19/75) i retko nakon autologne HSCT (2/29). Više od
50% slučajeva TI dijagnostikovano je tokom prvog meseca post-HSCT, pre engraftmenta odnosno
tokom uzdržavanja od profilakse. Sa druge strane, TI je dijagnostikovana kod 3/37 (8.1%) primalaca
HT. Uprkos primeni TMP-SMX, qPCR je postao pozitivan godinu dana posle HT kod dva i dve godine
post-HSCT kod trećeg pacijenta. Infekcija je bila preneta graftom kod 2/3 (seronegativni) a reaktivirana
kod 1/3 primalaca HT (seropozitivni primalac HT poreklom od seropozitivnog donora).
Naši rezultati potvrđuju da je sistemski qPCR monitoring iz uzoraka PB dragocen u dijagnostici TI
ne samo kod primalaca HSCT već i kod primalaca solidnih organa, posebno nakon HT. Učestalost
qPCR monitoringa se mora adaptirati shodno specifičnostima transplantacionog protokola, pre
svega primeni profilakse ali i osnovnoj dijagnozi, na način koji omogućava pravovremenu primenu
specifične terapije u svakom slučaju TI., Toxoplasmosis is a common but often neglected and misdiagnosed opportunistic infection in transplant
recipients, which can not only compromise the engraftment, but also evolve into life-threatening
disseminated infection. Post-transplantation, Toxoplasma gondii infection can develop as a reactivation
of chronic infection or could be graft-transmitted. We conducted an eight-year-long prospective
study on the diagnosis and monitoring of Toxoplasma infection (TI) in haematopoietic stem cell
transplant (HSCT) recipients in a setting that withholds prophylaxis until engraftment, and in heart
transplant (HT) recipients on continuous trimethoprim-sulfamethoxazole (TMP-SMX) prophylaxis.
The objective was to determine the incidence of TI before it evolves into clinical, potentially fatal
Toxoplasma disease (TD), in these two very different transplantation settings. Pre-transplantation
serological and qPCR screening was followed by post-transplantation peripheral blood (PB)-based
qPCR monitoring targeting the Toxoplasma 529 bp gene. In HSCT recipients, qPCR was performed
weekly while in HT recipients, qPCR was performed monthly for two months post-HT and then
yearly. TI was diagnosed based on a positive PCR result in at least one PB sample.
TI was diagnosed in 21/104 (20.2%) HSCT recipients, predominantly after allogeneic (19/75) and
rarely after autologous HSCT (2/29). Over 50% of TI cases were diagnosed during the first month
post-HSCT, while awaiting engraftment without prophylaxis. On the other hand, TI was diagnosed
in 3/37 (8.1%) HT recipients. Regardless of the TMP-SMX prophylaxis, qPCR became positive one
year after HT in two and two years post-HSCT in third patient. Infection was graft-transmitted in 2/3
(seronegative) and reactivated in 1/3 OHT (seropositive recipient of a seropositive donor’s heart
transplant).
The presented results show that systematic PB-based qPCR monitoring is a valuable resource for
the diagnosis of TI not only in HSCT but also in solid organ recipients, especially after HT. Frequency
of qPCR monitoring should be adjusted according to the specificity of the transplantation setting,
especially in terms of prophylaxis but also an underlying diagnosis, in a manner allowing for prompt
introduction of specific treatment in each case of TI.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije, Transplantation-related risk of Toxoplasma gondii infection: the National Reference Laboratory prospective cohort study results",
pages = "73-70",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1450"
}

Štajner, T., Vujić, D., Nestorović, E., Srbljanović, J., Bauman, N., Lijeskić, O., Zečević, Ž., Simić, M., Terzić, D., Jovanović, S., Dakić, Z.,& Bobić, B.. (2023). Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 70-73.
https://hdl.handle.net/21.15107/rcub_rimi_1450

Štajner T, Vujić D, Nestorović E, Srbljanović J, Bauman N, Lijeskić O, Zečević Ž, Simić M, Terzić D, Jovanović S, Dakić Z, Bobić B. Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:70-73.
https://hdl.handle.net/21.15107/rcub_rimi_1450 .

Štajner, Tijana, Vujić, Dragana, Nestorović, Emilija, Srbljanović, Jelena, Bauman, Neda, Lijeskić, Olivera, Zečević, Željko, Simić, Marija, Terzić, Duško, Jovanović, Snežana, Dakić, Zorica, Bobić, Branko, "Rizik od infekcije parazitom Toxoplasma gondii nakon transplantacije: rezultati prospektivne kohortne studije Nacionalne referentne laboratorije" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):70-73,
https://hdl.handle.net/21.15107/rcub_rimi_1450 .

TY  - CONF
AU  - Lijeskić, Olivera
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Bobić, Branko
AU  - Štajner, Tijana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1452
AB  - Implementacija treće doze vakcine protiv SARS-CoV-2 u preporuke širom sveta otvorila je polje
istraživanja heterologog pristupa revakcinaciji, odnosno kombinacije primarne serije vakcine i treće
doze različite vakcinalne platforme. Iako je literatura bogata radovima na temu heterologog pristupa,
imunogenost i trajanje humoralnog imunskog odgovora nakon kombinacije inaktivisane BBIBPCorV
i iRNK vakcine nisu dovoljno istraženi. Stoga, cilj ove studije bio je ispitivanje razlike u imunogenosti
i dugotrajnosti humoralnog imunskog odgovora u okviru perioda od šest meseci nakon
treće doze kod homologog (tri doze BNT162b2) i heterologog (BBIBP-CorV/BNT162b2) pristupa
revakcinaciji tokom Omikron talasa u Srbiji. U studiju je uključen 91 ispitanik, od kojih se 55 odlučilo
za homologi a 36 za heterologi pristup.
Serumi ispitanika analizirani su u četiri vremenske tačke: šest meseci nakon prve doze, a zatim tri nedelje,
tri meseca i šest meseci nakon treće doze. IgG antitela specifična za receptor-vezujući domen “šiljastog”
(eng. spike) proteina detektovana su BioMerieux VIDAS SARS-CoV-2 IgG testom. Tri nedelje
nakon treće doze, oba pristupa revakcinaciji dovela su do značajnog porasta u koncentraciji antitela
(p<0.0001). Štaviše, ispitanici koji su se opredelili za heterologu kombinaciju imali su statistički
značajno više koncentracije antitela od homologe grupe, u kontrolnim vremenskim tačkama na tri
nedelje i tri meseca nakon treće doze (p=0.025, p=0.0006). Međutim, značajan pad humoralnog
imunskog odgovora zapažen je tokom vremena kod oba pristupa. Većina infekcija nakon vakcinacije
registrovana je u periodu između tri i šest meseci nakon treće doze (n=22), a ukupna incidencija
ovih infekcija za posmatrani period iznosila je 36.36% (20/55) nakon homologog i 16.67% (6/36)
nakon heterologog pristupa.
Međutim, ispitanici sa potvrđenom infekcijom nakon vakcinacije nisu imali pneumoniju niti su bili
hospitalizovani. Iako je heterologi pristup indukovao više koncentracije antitela, naši rezultati ukazuju
da su i heterologi i homologi pristup indukovali potentan humoralni imunski odgovor i odgovarajuću
zaštitu od hospitalizacije i smrtnog ishoda tokom Omikron talasa. Međutim, opadanje imunskog
odgovora opaženo kod oba vakcinalna pristupa u periodu od šest meseci, kao i konstantna opasnost
od pojave novih pretećih varijanti, ukazuje na potrebu preispitivanja trenutne vakcinalne strategije.
AB  - Worldwide implementation of the third dose of vaccine against SARS-CoV-2 opened a new field of
research concerning the heterologous boost i.e., the combination of the primary vaccine series and
a different vaccinal platform for the third dose. Although literature is replete with studies of heterologous
boosts, longevity and immunogenicity of the inactivated BBIBP-CorV and mRNA BNT162b2
combination remains under-explored. Thus, the aim of this study was to evaluate the differences in
immunogenicity and longevity of the humoral immune response within six months after the third
dose in both homologous (BNT162b2) and heterologous (BBIBP-CorV/BNT162b2) vaccination setting,
and to assess the real-life data in the middle of the Omicron surge in Serbia.
A total of 91 individuals were included in this study, of which 55 received homologous and 36 heterologous
boost. Serum samples were analyzed at four timepoints: six months after the first dose;
three weeks, three months, and six months after the third dose. Specific IgG antibodies against the
receptor-binding domain of the spike protein were detected using BioMerieux VIDAS SARS-CoV-2
IgG kit. Both groups showed a highly significant increase in antibody concentrations (p<0.0001)
three weeks after the boost.
Furthermore, comparison per timepoint has shown that recipients of heterologous boost had significantly
higher antibody concentrations than homologous group, at three weeks and three months
after the boost (p=0.025, p=0.0006). However, a significant decline in antibody response over time
was noted for both strategies. The majority of breakthrough infections were registered in the period
between three and six months after the boost (n=22).Furthermore, total incidence was estimated at 36.36% (20/55) for homologous group, and 16.67%
(6/36) for heterologous group. Most importantly, none of the recipients of the third dose developed
pneumonia during the breakthrough infection, and none were hospitalized. In conclusion, although
heterologous approach resulted in higher antibody concentrations, our findings imply that both
homologous and heterologous boost induce potent humoral immune response and adequate protection
against hospitalization and death in the Omicron setting. However, waning immune
response registered for both types of boosts within six months and constant threats of new emerging
variants, calls for an update of vaccine strategy.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji
T1  - SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: homologous versus heterologous boost
EP  - 82
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1452
ER  - 

@conference{
author = "Lijeskić, Olivera and Srbljanović, Jelena and Bauman, Neda and Bobić, Branko and Štajner, Tijana",
year = "2023",
abstract = "Implementacija treće doze vakcine protiv SARS-CoV-2 u preporuke širom sveta otvorila je polje
istraživanja heterologog pristupa revakcinaciji, odnosno kombinacije primarne serije vakcine i treće
doze različite vakcinalne platforme. Iako je literatura bogata radovima na temu heterologog pristupa,
imunogenost i trajanje humoralnog imunskog odgovora nakon kombinacije inaktivisane BBIBPCorV
i iRNK vakcine nisu dovoljno istraženi. Stoga, cilj ove studije bio je ispitivanje razlike u imunogenosti
i dugotrajnosti humoralnog imunskog odgovora u okviru perioda od šest meseci nakon
treće doze kod homologog (tri doze BNT162b2) i heterologog (BBIBP-CorV/BNT162b2) pristupa
revakcinaciji tokom Omikron talasa u Srbiji. U studiju je uključen 91 ispitanik, od kojih se 55 odlučilo
za homologi a 36 za heterologi pristup.
Serumi ispitanika analizirani su u četiri vremenske tačke: šest meseci nakon prve doze, a zatim tri nedelje,
tri meseca i šest meseci nakon treće doze. IgG antitela specifična za receptor-vezujući domen “šiljastog”
(eng. spike) proteina detektovana su BioMerieux VIDAS SARS-CoV-2 IgG testom. Tri nedelje
nakon treće doze, oba pristupa revakcinaciji dovela su do značajnog porasta u koncentraciji antitela
(p<0.0001). Štaviše, ispitanici koji su se opredelili za heterologu kombinaciju imali su statistički
značajno više koncentracije antitela od homologe grupe, u kontrolnim vremenskim tačkama na tri
nedelje i tri meseca nakon treće doze (p=0.025, p=0.0006). Međutim, značajan pad humoralnog
imunskog odgovora zapažen je tokom vremena kod oba pristupa. Većina infekcija nakon vakcinacije
registrovana je u periodu između tri i šest meseci nakon treće doze (n=22), a ukupna incidencija
ovih infekcija za posmatrani period iznosila je 36.36% (20/55) nakon homologog i 16.67% (6/36)
nakon heterologog pristupa.
Međutim, ispitanici sa potvrđenom infekcijom nakon vakcinacije nisu imali pneumoniju niti su bili
hospitalizovani. Iako je heterologi pristup indukovao više koncentracije antitela, naši rezultati ukazuju
da su i heterologi i homologi pristup indukovali potentan humoralni imunski odgovor i odgovarajuću
zaštitu od hospitalizacije i smrtnog ishoda tokom Omikron talasa. Međutim, opadanje imunskog
odgovora opaženo kod oba vakcinalna pristupa u periodu od šest meseci, kao i konstantna opasnost
od pojave novih pretećih varijanti, ukazuje na potrebu preispitivanja trenutne vakcinalne strategije., Worldwide implementation of the third dose of vaccine against SARS-CoV-2 opened a new field of
research concerning the heterologous boost i.e., the combination of the primary vaccine series and
a different vaccinal platform for the third dose. Although literature is replete with studies of heterologous
boosts, longevity and immunogenicity of the inactivated BBIBP-CorV and mRNA BNT162b2
combination remains under-explored. Thus, the aim of this study was to evaluate the differences in
immunogenicity and longevity of the humoral immune response within six months after the third
dose in both homologous (BNT162b2) and heterologous (BBIBP-CorV/BNT162b2) vaccination setting,
and to assess the real-life data in the middle of the Omicron surge in Serbia.
A total of 91 individuals were included in this study, of which 55 received homologous and 36 heterologous
boost. Serum samples were analyzed at four timepoints: six months after the first dose;
three weeks, three months, and six months after the third dose. Specific IgG antibodies against the
receptor-binding domain of the spike protein were detected using BioMerieux VIDAS SARS-CoV-2
IgG kit. Both groups showed a highly significant increase in antibody concentrations (p<0.0001)
three weeks after the boost.
Furthermore, comparison per timepoint has shown that recipients of heterologous boost had significantly
higher antibody concentrations than homologous group, at three weeks and three months
after the boost (p=0.025, p=0.0006). However, a significant decline in antibody response over time
was noted for both strategies. The majority of breakthrough infections were registered in the period
between three and six months after the boost (n=22).Furthermore, total incidence was estimated at 36.36% (20/55) for homologous group, and 16.67%
(6/36) for heterologous group. Most importantly, none of the recipients of the third dose developed
pneumonia during the breakthrough infection, and none were hospitalized. In conclusion, although
heterologous approach resulted in higher antibody concentrations, our findings imply that both
homologous and heterologous boost induce potent humoral immune response and adequate protection
against hospitalization and death in the Omicron setting. However, waning immune
response registered for both types of boosts within six months and constant threats of new emerging
variants, calls for an update of vaccine strategy.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji, SARS-CoV-2 specific antibody response after an mRNA vaccine as the third dose: homologous versus heterologous boost",
pages = "82-79",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1452"
}

Lijeskić, O., Srbljanović, J., Bauman, N., Bobić, B.,& Štajner, T.. (2023). Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 79-82.
https://hdl.handle.net/21.15107/rcub_rimi_1452

Lijeskić O, Srbljanović J, Bauman N, Bobić B, Štajner T. Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:79-82.
https://hdl.handle.net/21.15107/rcub_rimi_1452 .

Lijeskić, Olivera, Srbljanović, Jelena, Bauman, Neda, Bobić, Branko, Štajner, Tijana, "Antitela specifična za SARS-CoV-2 nakon iRNK vakcine kao treće doze: homologi i heterologi pristup revakcinaciji" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):79-82,
https://hdl.handle.net/21.15107/rcub_rimi_1452 .

TY  - CONF
AU  - Srbljanović, Jelena
AU  - Štajner, Tijana
AU  - Bauman, Neda
AU  - Lijeskić, Olivera
AU  - Opsenica, Igor
AU  - Šolaja, Bogdan
AU  - Bobić, Branko
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1453
AB  - With an estimated 247 million cases annually and 619.000 deaths (in 2021) malaria remains a major
disease of the developing world and globally the most important parasitic disease. Because of
widespread resistance to available antimalarials including chloroquine (CQ) and its derivatives, new
drugs are urgently needed. Here we report on the antimalarial efficacy of new 4-aminoquinoline
derivatives, with modifications at the linker and at the quinoline nucleus.
In vitro screening was performed by the lactate dehydrogenase assay, based on measurement of the
plasmodial lactate dehydrogenase activity in both a CQ-sensitive (3D7) and a CQ-resistant (Dd2)
strain of Plasmodium falciparum, with a CQ as a control. In vivo antimalarial activity was investigated
in C57BL/6 mice infected with Plasmodium berghei ANKA strain by the modified Thompson test.
Compounds were first tested for toxicity. A total of 37 compounds were screened in vitro. Of the 22
that passed the first screening, 18 had IC50 values lower than CQ in the Dd2 strain while only one
was efficient in the 3D7 strain. However, even 15 compounds showed in vivo activity, significantly
(P<0.05) prolonging survival of treated vs. untreated mice. Among these, seven compounds afforded
the survival of 20–100% of treated mice up to Day 31, with or without the detection of parasites
in peripheral blood.
Most importantly, three of these, including ClAQ1, FClAQ1 and ClAQ8, afforded survival of 100% of
animals, the first two at 80 and 160 mg/kg/day and the last only at 160 mg/kg/day.Survival was associated with complete parasite clearance, as shown by both microscopy and qPCR.
Of note, continuous monitoring of parasitemia allowed the observation of a potentially important
phenomenon, that a number of compounds were able to confer resistance to cerebral malaria and
afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
By comparing the antimalarial activity of this group of novel compounds, we found that even minor
structural modifications substantially affect activity. The results of this extensive study are important,
as they may guide future work involving structural modifications of aminoquinolines, and as a contribution
to the knowledge in the field of malarial chemotherapy.
AB  - Malarija ostaje globalno najznačajnija parazitska infekcija sa procenjenih 247 miliona slučajeva
i 619.000 smrtnih slučajeva godišnje (2021.). Zbog široko rasprostranjene rezistencije na dostupne
antimalarike, uključujući hlorokvin (CQ) i njegove derivate, hitno su potrebni novi lekovi.
U ovom istraživanju ispitana je potencijalna antimalarijska aktivnost 37 novosintetisanih aminohinolina
sa hemijskim modifikacijama na aminohinolinskom jezgru i bočnom lancu. In vitro skrining aktivnosti
jedinjenja vršen je kolorimetrijskim esejom laktat dehidrogenaze na dva soja Plasmodium falciparum,
osetljivim (3D7) i rezistentnim (Dd2) na CQ, uz CQ kao pozitivnu kontrolu. Aktivnost u in
vivo sistemu je ispitana na ženkama miševa soja C57Bl/6 inficiranim ANKA sojem Plasmodium berghei
primenom modifikovanog Thompson-ovog testa. Ispitivanju aktivnosti jedinjenja prethodila je
faza kliničkog praćenja zdravih životinja terapiranih eksperimentalnim jedinjenjima. Od 37 jedinjenja
ispitanih u fazi in vitro skrininga, 22 koja su inhibirala ≥50% rast bar jednog od dva soja P. falciparum
odabrana su za titraciju do IC50 vrednosti.
Prema soju rezistentnom na CQ, 18 jedinjenja se pokazalo aktivnijim od CQ, dok je među njima samo
jedno jedinjenje bilo aktivnije i prema osetljivom soju. Čak 15 jedinjenja ispitanih u in vivo sistemu
značajno je produžilo život inficiranim životinjama u odnosu na kontrolnu grupu (P < 0.05).
Među njima, sedam jedinjenja je omogućilo preživljavanje 20–100% tretiranih miševa do dana 31, sa
ili bez nalaza parazita u perifernoj krvi. Posebno treba istaći tri jedinjenja koja su dovela do izlečenja
svih tretiranih životinja, ClAQ1 i FClAQ1 (80 i 160 mg/kg/dan) i ClAQ8 (160 mg/kg/dan).Preživljavanje je bilo praćeno i kompletnim klirensom parazita što je dokazano mikroskopskim pregledom
razmaza kao i qPCR analizom krvi i tkiva jetre preživelih životinja. Važno je pomenuti da
je kontinuirano praćenje parazitemije svih tretiranih miševa omogućilo da se zapazi potencijalno
značajan fenomen.
Naime, neka jedinjenja su omogućila da miševi postanu otporni na razvoj cerebralne malarije
i uzrokovala da miševi skloni razvoju neurološkog sindroma tolerišu preživljavanje sa izuzetno velikim
brojem parazita. Poređenjem antimalarijske aktivnosti novosintetisanih aminohinolina uočeno je da
i male strukturne promene u velikoj meri menjaju aktivnost. Rezultati ovog opsežnog istraživanja
su od značaja za buduća istraživanja strukturne modifikacije aminohinolina i doprinose proširenju
znanja u oblasti hemioterapije malarije.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Experimental treatment of malaria – new perspectives
T1  - Eksperimentalna terapija malarije – novi vidici
EP  - 92
SP  - 89
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1453
ER  - 

@conference{
author = "Srbljanović, Jelena and Štajner, Tijana and Bauman, Neda and Lijeskić, Olivera and Opsenica, Igor and Šolaja, Bogdan and Bobić, Branko",
year = "2023",
abstract = "With an estimated 247 million cases annually and 619.000 deaths (in 2021) malaria remains a major
disease of the developing world and globally the most important parasitic disease. Because of
widespread resistance to available antimalarials including chloroquine (CQ) and its derivatives, new
drugs are urgently needed. Here we report on the antimalarial efficacy of new 4-aminoquinoline
derivatives, with modifications at the linker and at the quinoline nucleus.
In vitro screening was performed by the lactate dehydrogenase assay, based on measurement of the
plasmodial lactate dehydrogenase activity in both a CQ-sensitive (3D7) and a CQ-resistant (Dd2)
strain of Plasmodium falciparum, with a CQ as a control. In vivo antimalarial activity was investigated
in C57BL/6 mice infected with Plasmodium berghei ANKA strain by the modified Thompson test.
Compounds were first tested for toxicity. A total of 37 compounds were screened in vitro. Of the 22
that passed the first screening, 18 had IC50 values lower than CQ in the Dd2 strain while only one
was efficient in the 3D7 strain. However, even 15 compounds showed in vivo activity, significantly
(P<0.05) prolonging survival of treated vs. untreated mice. Among these, seven compounds afforded
the survival of 20–100% of treated mice up to Day 31, with or without the detection of parasites
in peripheral blood.
Most importantly, three of these, including ClAQ1, FClAQ1 and ClAQ8, afforded survival of 100% of
animals, the first two at 80 and 160 mg/kg/day and the last only at 160 mg/kg/day.Survival was associated with complete parasite clearance, as shown by both microscopy and qPCR.
Of note, continuous monitoring of parasitemia allowed the observation of a potentially important
phenomenon, that a number of compounds were able to confer resistance to cerebral malaria and
afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
By comparing the antimalarial activity of this group of novel compounds, we found that even minor
structural modifications substantially affect activity. The results of this extensive study are important,
as they may guide future work involving structural modifications of aminoquinolines, and as a contribution
to the knowledge in the field of malarial chemotherapy., Malarija ostaje globalno najznačajnija parazitska infekcija sa procenjenih 247 miliona slučajeva
i 619.000 smrtnih slučajeva godišnje (2021.). Zbog široko rasprostranjene rezistencije na dostupne
antimalarike, uključujući hlorokvin (CQ) i njegove derivate, hitno su potrebni novi lekovi.
U ovom istraživanju ispitana je potencijalna antimalarijska aktivnost 37 novosintetisanih aminohinolina
sa hemijskim modifikacijama na aminohinolinskom jezgru i bočnom lancu. In vitro skrining aktivnosti
jedinjenja vršen je kolorimetrijskim esejom laktat dehidrogenaze na dva soja Plasmodium falciparum,
osetljivim (3D7) i rezistentnim (Dd2) na CQ, uz CQ kao pozitivnu kontrolu. Aktivnost u in
vivo sistemu je ispitana na ženkama miševa soja C57Bl/6 inficiranim ANKA sojem Plasmodium berghei
primenom modifikovanog Thompson-ovog testa. Ispitivanju aktivnosti jedinjenja prethodila je
faza kliničkog praćenja zdravih životinja terapiranih eksperimentalnim jedinjenjima. Od 37 jedinjenja
ispitanih u fazi in vitro skrininga, 22 koja su inhibirala ≥50% rast bar jednog od dva soja P. falciparum
odabrana su za titraciju do IC50 vrednosti.
Prema soju rezistentnom na CQ, 18 jedinjenja se pokazalo aktivnijim od CQ, dok je među njima samo
jedno jedinjenje bilo aktivnije i prema osetljivom soju. Čak 15 jedinjenja ispitanih u in vivo sistemu
značajno je produžilo život inficiranim životinjama u odnosu na kontrolnu grupu (P < 0.05).
Među njima, sedam jedinjenja je omogućilo preživljavanje 20–100% tretiranih miševa do dana 31, sa
ili bez nalaza parazita u perifernoj krvi. Posebno treba istaći tri jedinjenja koja su dovela do izlečenja
svih tretiranih životinja, ClAQ1 i FClAQ1 (80 i 160 mg/kg/dan) i ClAQ8 (160 mg/kg/dan).Preživljavanje je bilo praćeno i kompletnim klirensom parazita što je dokazano mikroskopskim pregledom
razmaza kao i qPCR analizom krvi i tkiva jetre preživelih životinja. Važno je pomenuti da
je kontinuirano praćenje parazitemije svih tretiranih miševa omogućilo da se zapazi potencijalno
značajan fenomen.
Naime, neka jedinjenja su omogućila da miševi postanu otporni na razvoj cerebralne malarije
i uzrokovala da miševi skloni razvoju neurološkog sindroma tolerišu preživljavanje sa izuzetno velikim
brojem parazita. Poređenjem antimalarijske aktivnosti novosintetisanih aminohinolina uočeno je da
i male strukturne promene u velikoj meri menjaju aktivnost. Rezultati ovog opsežnog istraživanja
su od značaja za buduća istraživanja strukturne modifikacije aminohinolina i doprinose proširenju
znanja u oblasti hemioterapije malarije.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Experimental treatment of malaria – new perspectives, Eksperimentalna terapija malarije – novi vidici",
pages = "92-89",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1453"
}

Srbljanović, J., Štajner, T., Bauman, N., Lijeskić, O., Opsenica, I., Šolaja, B.,& Bobić, B.. (2023). Experimental treatment of malaria – new perspectives. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 89-92.
https://hdl.handle.net/21.15107/rcub_rimi_1453

Srbljanović J, Štajner T, Bauman N, Lijeskić O, Opsenica I, Šolaja B, Bobić B. Experimental treatment of malaria – new perspectives. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:89-92.
https://hdl.handle.net/21.15107/rcub_rimi_1453 .

Srbljanović, Jelena, Štajner, Tijana, Bauman, Neda, Lijeskić, Olivera, Opsenica, Igor, Šolaja, Bogdan, Bobić, Branko, "Experimental treatment of malaria – new perspectives" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):89-92,
https://hdl.handle.net/21.15107/rcub_rimi_1453 .

TY  - JOUR
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Ćirković, Vladimir
AU  - Bauman, Neda
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Lijeskić, Olivera
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1190
AB  - In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.
PB  - MDPI
T2  - Microorganisms
T1  - Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange
IS  - 12
SP  - 2526
VL  - 9
DO  - 10.3390/microorganisms9122526
ER  - 

@article{
author = "Uzelac, Aleksandra and Klun, Ivana and Ćirković, Vladimir and Bauman, Neda and Bobić, Branko and Štajner, Tijana and Srbljanović, Jelena and Lijeskić, Olivera and Đurković-Đaković, Olgica",
year = "2021",
abstract = "In Europe, Toxoplasma gondii lineage II is dominant, and ToxoDB#1 the most frequently occurring genotype. The abundance of lineage III genotypes varies geographically and lineage I are rare, yet present in several regions of the continent. Data on the T. gondii population structure in southeastern Europe (SEE) are scarce, yet necessary to appreciate the diversity of the species in Europe. To help fill this gap, we genotyped 67 strains from nine species of intermediate hosts in Serbia by MnPCR-RFLP, determined the population structure, and identified the genotypes using ToxoDB. A neighbor-joining tree was also constructed from the isolates genotyped on nine loci. While 42% of the total genotype population consisted of ToxoDB#1 and ToxoDB#2, variant genotypes of both lineages comprised 46% of the population in wildlife and 28% in domestic animals and humans. One genotype of Africa 4 lineage was detected in a human sample. Interestingly, the findings include one lineage III variant and one II/III recombinant isolate with intercontinental distribution, which appear to be moderately related to South American genotypes. Based on these findings, SEE is a region of underappreciated T. gondii genetic diversity and possible strain exchange between Europe and Africa.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange",
number = "12",
pages = "2526",
volume = "9",
doi = "10.3390/microorganisms9122526"
}

Uzelac, A., Klun, I., Ćirković, V., Bauman, N., Bobić, B., Štajner, T., Srbljanović, J., Lijeskić, O.,& Đurković-Đaković, O.. (2021). Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms
MDPI., 9(12), 2526.
https://doi.org/10.3390/microorganisms9122526

Uzelac A, Klun I, Ćirković V, Bauman N, Bobić B, Štajner T, Srbljanović J, Lijeskić O, Đurković-Đaković O. Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange. in Microorganisms. 2021;9(12):2526.
doi:10.3390/microorganisms9122526 .

Uzelac, Aleksandra, Klun, Ivana, Ćirković, Vladimir, Bauman, Neda, Bobić, Branko, Štajner, Tijana, Srbljanović, Jelena, Lijeskić, Olivera, Đurković-Đaković, Olgica, "Toxoplasma gondii Genotypes Circulating in Serbia—Insight into the Population Structure and Diversity of the Species in Southeastern Europe, a Region of Intercontinental Strain Exchange" in Microorganisms, 9, no. 12 (2021):2526,
https://doi.org/10.3390/microorganisms9122526 . .

TY  - JOUR
AU  - Bobić, Branko
AU  - Ćirković, Vladimir
AU  - Klun, Ivana
AU  - Štajner, Tijana
AU  - Srbljanović, Jelena
AU  - Bauman, Neda
AU  - Đurković-Đaković, Olgica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1267
AB  - Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.
PB  - Cambridge University Press
T2  - Journal of Helminthology
T1  - Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review
SP  - e49
VL  - 95
DO  - 10.1017/S0022149X21000432
ER  - 

@article{
author = "Bobić, Branko and Ćirković, Vladimir and Klun, Ivana and Štajner, Tijana and Srbljanović, Jelena and Bauman, Neda and Đurković-Đaković, Olgica",
year = "2021",
abstract = "Taenia solium is a zoonotic parasite that causes taeniasis and cysticercosis in humans (as final hosts) and cysticercosis in pigs (as intermediate hosts). The Russian Federation (RF) is traditionally considered as endemic for this zoonosis. However, the epidemiological data on T. solium infection have not been reviewed for the past 20 years, in which time dynamic economical and societal changes have occurred in the RF. The aim of this systematic review was to analyse the status of T. solium infection in RF in the 2000–2019 period. A literature search was conducted, which collected published articles, grey literature and official data on the epidemiology of T. solium taeniasis and cysticercosis in the RF published from 2000. From a total of 2021 articles and 24 official reports originally returned by the search, data were extracted from 12 full text articles and 11 official reports. Taenia solium taeniasis was continuously reported in the RF between 2000 and 2019, with a tenfold decrease in the incidence, from 0.2 per 100,000 population in 2000 to 0.023/100,000 in 2019. Also, the number of administrative units where taeniasis was detected continuously decreased. Cysticercosis in pigs had a declining trend after 2006. In conclusion, although decreasing, T. solium infection is still endemic in several regions and suspected to be endemic in most of the RF.",
publisher = "Cambridge University Press",
journal = "Journal of Helminthology",
title = "Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review",
pages = "e49",
volume = "95",
doi = "10.1017/S0022149X21000432"
}

Bobić, B., Ćirković, V., Klun, I., Štajner, T., Srbljanović, J., Bauman, N.,& Đurković-Đaković, O.. (2021). Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review. in Journal of Helminthology
Cambridge University Press., 95, e49.
https://doi.org/10.1017/S0022149X21000432

Bobić B, Ćirković V, Klun I, Štajner T, Srbljanović J, Bauman N, Đurković-Đaković O. Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review. in Journal of Helminthology. 2021;95:e49.
doi:10.1017/S0022149X21000432 .

Bobić, Branko, Ćirković, Vladimir, Klun, Ivana, Štajner, Tijana, Srbljanović, Jelena, Bauman, Neda, Đurković-Đaković, Olgica, "Epidemiology of Taenia solium infection in the Russian Federation in the last 20 years: a systematic review" in Journal of Helminthology, 95 (2021):e49,
https://doi.org/10.1017/S0022149X21000432 . .

TY  - JOUR
AU  - Srbljanović, Jelena
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Uzelac, Aleksandra
AU  - Opsenica, Igor
AU  - Terzić-Jovanović, Nataša
AU  - Bauman, Neda
AU  - Šolaja, Bogdan
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/998
AB  - Objectives: Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. Methods: In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 and CQ-resistant (CQ(R)) P. falciparum strain Dd2. In vivo activity was evaluated by a modified Thompson test using C57BL/6 mice infected with Plasmodium berghei ANKA strain. Results: Nine of the ten compounds had a lower 50% inhibitory concentration (IC50) than CQ against the CQ(R) strain Dd2. Five of these compounds that were available for in vivo evaluation were shown to be nontoxic. All five compounds administered at a dose of 160 mg/kg/day for 3 days prolonged the survival of treated compared with untreated mice. Untreated control mice died by Day 7 with a mean parasitaemia of 15%. Among treated mice, a dichotomous outcome was observed, with a two-third majority of treated mice dying by Day 17 with a low mean parasitaemia of 5%, whilst one-third survived longer with a mean hyperparasitaemia of 70%; specifically, five of these mice survived a mean of 25 days, whilst two even survived past Day 31. Conclusions: The significant antimalarial potential of this aminoquinoline series is illustrated by its excellent in vitro activity against the CQ(R) P. falciparum strain and significant in vivo activity. Interestingly, compounds CIAQ7, CIAQ9 and CIAQ11 were able to confer resistance to cerebral malaria and afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Global Antimicrobial Resistance
T1  - Aminoquinolines afford resistance to cerebral malaria in susceptible mice
EP  - 25
SP  - 20
VL  - 23
DO  - 10.1016/j.jgar.2020.07.027
ER  - 

@article{
author = "Srbljanović, Jelena and Bobić, Branko and Štajner, Tijana and Uzelac, Aleksandra and Opsenica, Igor and Terzić-Jovanović, Nataša and Bauman, Neda and Šolaja, Bogdan and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Objectives: Malaria treatment is impeded by increasing resistance to conventional antimalarial drugs. Here we explored the activity of ten novel benzothiophene, thiophene and benzene aminoquinolines. Methods: In vitro testing was performed by the lactate dehydrogenase assay in chloroquine (CQ)-sensitive Plasmodium falciparum strain 3D7 and CQ-resistant (CQ(R)) P. falciparum strain Dd2. In vivo activity was evaluated by a modified Thompson test using C57BL/6 mice infected with Plasmodium berghei ANKA strain. Results: Nine of the ten compounds had a lower 50% inhibitory concentration (IC50) than CQ against the CQ(R) strain Dd2. Five of these compounds that were available for in vivo evaluation were shown to be nontoxic. All five compounds administered at a dose of 160 mg/kg/day for 3 days prolonged the survival of treated compared with untreated mice. Untreated control mice died by Day 7 with a mean parasitaemia of 15%. Among treated mice, a dichotomous outcome was observed, with a two-third majority of treated mice dying by Day 17 with a low mean parasitaemia of 5%, whilst one-third survived longer with a mean hyperparasitaemia of 70%; specifically, five of these mice survived a mean of 25 days, whilst two even survived past Day 31. Conclusions: The significant antimalarial potential of this aminoquinoline series is illustrated by its excellent in vitro activity against the CQ(R) P. falciparum strain and significant in vivo activity. Interestingly, compounds CIAQ7, CIAQ9 and CIAQ11 were able to confer resistance to cerebral malaria and afford a switch to hyperparasitaemia to mice prone to the neurological syndrome.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Global Antimicrobial Resistance",
title = "Aminoquinolines afford resistance to cerebral malaria in susceptible mice",
pages = "25-20",
volume = "23",
doi = "10.1016/j.jgar.2020.07.027"
}

Srbljanović, J., Bobić, B., Štajner, T., Uzelac, A., Opsenica, I., Terzić-Jovanović, N., Bauman, N., Šolaja, B.,& Đurković-Đaković, O.. (2020). Aminoquinolines afford resistance to cerebral malaria in susceptible mice. in Journal of Global Antimicrobial Resistance
Elsevier Sci Ltd, Oxford., 23, 20-25.
https://doi.org/10.1016/j.jgar.2020.07.027

Srbljanović J, Bobić B, Štajner T, Uzelac A, Opsenica I, Terzić-Jovanović N, Bauman N, Šolaja B, Đurković-Đaković O. Aminoquinolines afford resistance to cerebral malaria in susceptible mice. in Journal of Global Antimicrobial Resistance. 2020;23:20-25.
doi:10.1016/j.jgar.2020.07.027 .

Srbljanović, Jelena, Bobić, Branko, Štajner, Tijana, Uzelac, Aleksandra, Opsenica, Igor, Terzić-Jovanović, Nataša, Bauman, Neda, Šolaja, Bogdan, Đurković-Đaković, Olgica, "Aminoquinolines afford resistance to cerebral malaria in susceptible mice" in Journal of Global Antimicrobial Resistance, 23 (2020):20-25,
https://doi.org/10.1016/j.jgar.2020.07.027 . .

TY  - JOUR
AU  - Bauman, Neda
AU  - Ilić, Anđelija
AU  - Lijeskić, Olivera
AU  - Uzelac, Aleksandra
AU  - Klun, Ivana
AU  - Srbljanović, Jelena
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Štajner, Tijana
AU  - Đurković-Đaković, Olgica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1046
AB  - Toxoplasma gondiiis an obligate intracellular parasite infecting up to one third of the human population. The central event in the pathogenesis of toxoplasmosis is the conversion of tachyzoites into encysted bradyzoites. A novel approach to analyze the structure ofin vivo-derived tissue cysts may be the increasingly used computational image analysis. The objective of this study was to quantify the geometrical complexity ofT.gondiicysts by morphological, particle, and fractal analysis, as well as to determine if it is impacted by parasite strain, cyst age, and host type. A total of 31 images ofT.gondiibrain cysts of four type-2 strains (Me49, and local isolates BGD1, BGD14, and BGD26) was analyzed using ImageJ software. The parameters of interest included diameter, circularity, packing density (PD), fractal dimension (FD), and lacunarity. Although cyst diameter varied widely, its negative correlation with PD was observed. Circularity was remarkably close to 1, indicating a perfectly round shape of the cysts. PD and FD did not vary among cysts of different strains, age, and derived from mice of different genetic background. Conversely, lacunarity, which is a measure of heterogeneity, was significantly lower for BGD1 strain vs. all other strains, and higher for Me49 vs. BGD14 and BGD26, but did not differ among Me49 cysts of different age, or those derived from genetically different mice. The results indicate a highly uniform structure and occupancy of the differentT.gondiitissue cysts. This study furthers the use of image analysis in describing the structural complexity ofT.gondiicyst morphology, and presents the first application of fractal analysis for this purpose. The presented results show that use of a freely available software is a cost-effective approach to advance automated image scoring forT.gondiicysts.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts
IS  - 8
SP  - e0234169
VL  - 15
DO  - 10.1371/journal.pone.0234169
ER  - 

@article{
author = "Bauman, Neda and Ilić, Anđelija and Lijeskić, Olivera and Uzelac, Aleksandra and Klun, Ivana and Srbljanović, Jelena and Ćirković, Vladimir and Bobić, Branko and Štajner, Tijana and Đurković-Đaković, Olgica",
year = "2020",
abstract = "Toxoplasma gondiiis an obligate intracellular parasite infecting up to one third of the human population. The central event in the pathogenesis of toxoplasmosis is the conversion of tachyzoites into encysted bradyzoites. A novel approach to analyze the structure ofin vivo-derived tissue cysts may be the increasingly used computational image analysis. The objective of this study was to quantify the geometrical complexity ofT.gondiicysts by morphological, particle, and fractal analysis, as well as to determine if it is impacted by parasite strain, cyst age, and host type. A total of 31 images ofT.gondiibrain cysts of four type-2 strains (Me49, and local isolates BGD1, BGD14, and BGD26) was analyzed using ImageJ software. The parameters of interest included diameter, circularity, packing density (PD), fractal dimension (FD), and lacunarity. Although cyst diameter varied widely, its negative correlation with PD was observed. Circularity was remarkably close to 1, indicating a perfectly round shape of the cysts. PD and FD did not vary among cysts of different strains, age, and derived from mice of different genetic background. Conversely, lacunarity, which is a measure of heterogeneity, was significantly lower for BGD1 strain vs. all other strains, and higher for Me49 vs. BGD14 and BGD26, but did not differ among Me49 cysts of different age, or those derived from genetically different mice. The results indicate a highly uniform structure and occupancy of the differentT.gondiitissue cysts. This study furthers the use of image analysis in describing the structural complexity ofT.gondiicyst morphology, and presents the first application of fractal analysis for this purpose. The presented results show that use of a freely available software is a cost-effective approach to advance automated image scoring forT.gondiicysts.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts",
number = "8",
pages = "e0234169",
volume = "15",
doi = "10.1371/journal.pone.0234169"
}

Bauman, N., Ilić, A., Lijeskić, O., Uzelac, A., Klun, I., Srbljanović, J., Ćirković, V., Bobić, B., Štajner, T.,& Đurković-Đaković, O.. (2020). Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts. in PLoS One
Public Library Science, San Francisco., 15(8), e0234169.
https://doi.org/10.1371/journal.pone.0234169

Bauman N, Ilić A, Lijeskić O, Uzelac A, Klun I, Srbljanović J, Ćirković V, Bobić B, Štajner T, Đurković-Đaković O. Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts. in PLoS One. 2020;15(8):e0234169.
doi:10.1371/journal.pone.0234169 .

Bauman, Neda, Ilić, Anđelija, Lijeskić, Olivera, Uzelac, Aleksandra, Klun, Ivana, Srbljanović, Jelena, Ćirković, Vladimir, Bobić, Branko, Štajner, Tijana, Đurković-Đaković, Olgica, "Computational image analysis reveals the structural complexity ofToxoplasma gondiitissue cysts" in PLoS One, 15, no. 8 (2020):e0234169,
https://doi.org/10.1371/journal.pone.0234169 . .

TY  - JOUR
AU  - Stopić, Milena
AU  - Bobić, Branko
AU  - Dakić, Zorica
AU  - Srbljanović, Jelena
AU  - Štajner, Tijana
AU  - Konstantinović, Neda M.
AU  - Srećković, Katarina
AU  - Klun, Ivana
AU  - Korac, Miloš
AU  - Đurković-Đaković, Olgica
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/909
AB  - Objectives: As is the case for all of Southeast Europe, Serbia is an area traditionally endemic for Taenia saginata and Taenia solium infections. This study was performed to analyse the epidemiological data on taeniosis and cysticercosis in Serbia for the period 1990-2018. Methods: Data on cases of T. saginata and T. solium infection were collected via a systematic search of published articles, the grey literature, and official reports, as well as by performing clinical observational studies of patients treated in the departments for infectious diseases of hospitals and university clinics in Serbia. Results: A total of 212 cases of taeniosis were reported, all between 1997 and 2004 when taeniosis was notifiable (incidence range 0.04-0.9/100 000 population/year). From 1990 to 2018, 170 cases of cysticercosis (all but one of neurocysticercosis), were registered (incidence range 0-0.29/100 000 population/year), with a strong decrease since 2000 and a single case in the last 9 years. The annual number of cases of both taeniosis (Pearson's r = 0.914, p = 0.001) and cysticercosis (Pearson's r = 0.582, p = 0.014) correlated with the consumer price index. Conclusions: In Serbia, T. saginata and T. solium infections are autochthonous but occur only sporadically. However, the potential for re-emergence exists, depending on the socio-economic state of the country.
PB  - Elsevier Sci Ltd, Oxford
T2  - International Journal of Infectious Diseases
T1  - Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living
EP  - 141
SP  - 135
VL  - 86
DO  - 10.1016/j.ijid.2019.07.010
ER  - 

@article{
author = "Stopić, Milena and Bobić, Branko and Dakić, Zorica and Srbljanović, Jelena and Štajner, Tijana and Konstantinović, Neda M. and Srećković, Katarina and Klun, Ivana and Korac, Miloš and Đurković-Đaković, Olgica",
year = "2019",
abstract = "Objectives: As is the case for all of Southeast Europe, Serbia is an area traditionally endemic for Taenia saginata and Taenia solium infections. This study was performed to analyse the epidemiological data on taeniosis and cysticercosis in Serbia for the period 1990-2018. Methods: Data on cases of T. saginata and T. solium infection were collected via a systematic search of published articles, the grey literature, and official reports, as well as by performing clinical observational studies of patients treated in the departments for infectious diseases of hospitals and university clinics in Serbia. Results: A total of 212 cases of taeniosis were reported, all between 1997 and 2004 when taeniosis was notifiable (incidence range 0.04-0.9/100 000 population/year). From 1990 to 2018, 170 cases of cysticercosis (all but one of neurocysticercosis), were registered (incidence range 0-0.29/100 000 population/year), with a strong decrease since 2000 and a single case in the last 9 years. The annual number of cases of both taeniosis (Pearson's r = 0.914, p = 0.001) and cysticercosis (Pearson's r = 0.582, p = 0.014) correlated with the consumer price index. Conclusions: In Serbia, T. saginata and T. solium infections are autochthonous but occur only sporadically. However, the potential for re-emergence exists, depending on the socio-economic state of the country.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "International Journal of Infectious Diseases",
title = "Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living",
pages = "141-135",
volume = "86",
doi = "10.1016/j.ijid.2019.07.010"
}

Stopić, M., Bobić, B., Dakić, Z., Srbljanović, J., Štajner, T., Konstantinović, N. M., Srećković, K., Klun, I., Korac, M.,& Đurković-Đaković, O.. (2019). Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living. in International Journal of Infectious Diseases
Elsevier Sci Ltd, Oxford., 86, 135-141.
https://doi.org/10.1016/j.ijid.2019.07.010

Stopić M, Bobić B, Dakić Z, Srbljanović J, Štajner T, Konstantinović NM, Srećković K, Klun I, Korac M, Đurković-Đaković O. Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living. in International Journal of Infectious Diseases. 2019;86:135-141.
doi:10.1016/j.ijid.2019.07.010 .

Stopić, Milena, Bobić, Branko, Dakić, Zorica, Srbljanović, Jelena, Štajner, Tijana, Konstantinović, Neda M., Srećković, Katarina, Klun, Ivana, Korac, Miloš, Đurković-Đaković, Olgica, "Taeniosis and cysticercosis in Serbia, 1990-2018: Significance of standard of living" in International Journal of Infectious Diseases, 86 (2019):135-141,
https://doi.org/10.1016/j.ijid.2019.07.010 . .

TY  - JOUR
AU  - Konstantinović, Neda M.
AU  - Guegan, Helene
AU  - Štajner, Tijana
AU  - Belaz, S.
AU  - Robert-Gangneux, Florence
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/959
AB  - Toxoplasmosis is a worldwide parasitic disease infecting about one third of humans, with possible severe outcomes in neonates and immunocompromised patients. Despite continuous and successful efforts to improve diagnosis, therapeutic schemes have barely evolved since many years. This article aims at reviewing the main clinical trials and current treatment practices, and at addressing future perspectives in the light of ongoing researches.
T2  - Food & Waterborne Parasitology
T1  - Treatment of toxoplasmosis: Current options and future perspectives
VL  - 15
DO  - 10.1016/j.fawpar.2019.e00036
ER  - 

@article{
author = "Konstantinović, Neda M. and Guegan, Helene and Štajner, Tijana and Belaz, S. and Robert-Gangneux, Florence",
year = "2019",
abstract = "Toxoplasmosis is a worldwide parasitic disease infecting about one third of humans, with possible severe outcomes in neonates and immunocompromised patients. Despite continuous and successful efforts to improve diagnosis, therapeutic schemes have barely evolved since many years. This article aims at reviewing the main clinical trials and current treatment practices, and at addressing future perspectives in the light of ongoing researches.",
journal = "Food & Waterborne Parasitology",
title = "Treatment of toxoplasmosis: Current options and future perspectives",
volume = "15",
doi = "10.1016/j.fawpar.2019.e00036"
}

Konstantinović, N. M., Guegan, H., Štajner, T., Belaz, S.,& Robert-Gangneux, F.. (2019). Treatment of toxoplasmosis: Current options and future perspectives. in Food & Waterborne Parasitology, 15.
https://doi.org/10.1016/j.fawpar.2019.e00036

Konstantinović NM, Guegan H, Štajner T, Belaz S, Robert-Gangneux F. Treatment of toxoplasmosis: Current options and future perspectives. in Food & Waterborne Parasitology. 2019;15.
doi:10.1016/j.fawpar.2019.e00036 .

Konstantinović, Neda M., Guegan, Helene, Štajner, Tijana, Belaz, S., Robert-Gangneux, Florence, "Treatment of toxoplasmosis: Current options and future perspectives" in Food & Waterborne Parasitology, 15 (2019),
https://doi.org/10.1016/j.fawpar.2019.e00036 . .

TY  - JOUR
AU  - Ćirković, Ivana
AU  - Jocić, Dario
AU  - Božić, Dragana D.
AU  - Đukić, Slobodanka
AU  - Konstantinović, Neda M.
AU  - Radak, Đorđe
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/878
AB  - Biofilm-associated wound infections are a major global health issue, and methicillin-resistant Staphylococcus aureus (MRSA) is among the greatest therapeutic challenges. Vacuum-assisted closure (VAC) therapy is now being revisited as an alternative treatment for both acute and chronic wounds. However, data supporting the concept of its antibiofilm effect remain limited. Using quantitative biofilm-forming assay and a range of genotypic methods (spa, SCCmec, and agr typing), study authors showed that VAC therapy can significantly prevent biofilm formation (P  lt  .01) of a range of MRSA wound isolates differing widely in their biofilm-forming abilities and genetic background. The best effect was presented on CC5-MRSA-SCCmecI-agrII, a dominant MRSA clone among wound isolates worldwide. An assessment of effects of different protocols on dressing changes (1 or 2 times per week) demonstrated significantly greater antibiofilm activity (P  lt  .05) of 3-day dressing changes. These findings support the use of VAC therapy as a topical antibiofilm treatment for the effective management of wound healing.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Advances in Skin & Wound Care
T1  - The Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound Biofilms
EP  - 364
IS  - 8
SP  - 361
VL  - 31
DO  - 10.1097/01.ASW.0000540070.07040.70
ER  - 

@article{
author = "Ćirković, Ivana and Jocić, Dario and Božić, Dragana D. and Đukić, Slobodanka and Konstantinović, Neda M. and Radak, Đorđe",
year = "2018",
abstract = "Biofilm-associated wound infections are a major global health issue, and methicillin-resistant Staphylococcus aureus (MRSA) is among the greatest therapeutic challenges. Vacuum-assisted closure (VAC) therapy is now being revisited as an alternative treatment for both acute and chronic wounds. However, data supporting the concept of its antibiofilm effect remain limited. Using quantitative biofilm-forming assay and a range of genotypic methods (spa, SCCmec, and agr typing), study authors showed that VAC therapy can significantly prevent biofilm formation (P  lt  .01) of a range of MRSA wound isolates differing widely in their biofilm-forming abilities and genetic background. The best effect was presented on CC5-MRSA-SCCmecI-agrII, a dominant MRSA clone among wound isolates worldwide. An assessment of effects of different protocols on dressing changes (1 or 2 times per week) demonstrated significantly greater antibiofilm activity (P  lt  .05) of 3-day dressing changes. These findings support the use of VAC therapy as a topical antibiofilm treatment for the effective management of wound healing.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Advances in Skin & Wound Care",
title = "The Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound Biofilms",
pages = "364-361",
number = "8",
volume = "31",
doi = "10.1097/01.ASW.0000540070.07040.70"
}

Ćirković, I., Jocić, D., Božić, D. D., Đukić, S., Konstantinović, N. M.,& Radak, Đ.. (2018). The Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound Biofilms. in Advances in Skin & Wound Care
Lippincott Williams & Wilkins, Philadelphia., 31(8), 361-364.
https://doi.org/10.1097/01.ASW.0000540070.07040.70

Ćirković I, Jocić D, Božić DD, Đukić S, Konstantinović NM, Radak Đ. The Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound Biofilms. in Advances in Skin & Wound Care. 2018;31(8):361-364.
doi:10.1097/01.ASW.0000540070.07040.70 .

Ćirković, Ivana, Jocić, Dario, Božić, Dragana D., Đukić, Slobodanka, Konstantinović, Neda M., Radak, Đorđe, "The Effect of Vacuum-Assisted Closure Therapy on Methicillin-Resistant Staphylococcus aureus Wound Biofilms" in Advances in Skin & Wound Care, 31, no. 8 (2018):361-364,
https://doi.org/10.1097/01.ASW.0000540070.07040.70 . .

TY  - JOUR
AU  - Konstantinović, Neda M.
AU  - Ćirković, Ivana
AU  - Đukić, Slobodanka
AU  - Marić, Vesna
AU  - Božić, Dragana D.
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/756
AB  - Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Biofilm formation of achromobacter xylosoxidans on contact lens
EP  - 300
IS  - 3
SP  - 293
VL  - 64
DO  - 10.1556/030.64.2017.005
ER  - 

@article{
author = "Konstantinović, Neda M. and Ćirković, Ivana and Đukić, Slobodanka and Marić, Vesna and Božić, Dragana D.",
year = "2017",
abstract = "Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated strain of A. xylosoxidans and reference strains of P. aeruginosa, S. aureus, and H. influenzae on soft CL were analyzed by commonly used microtitre plate method. Our results showed that isolated strain of A. xylosoxidans was capable to form biofilm on the surface of soft contact lens. A. xylosoxidans was strong biofilm producer while all examined reference strains were moderate biofilm producers. A. xylosoxidans appears to be superior biofilm producer on soft CL compared to reference strains.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Biofilm formation of achromobacter xylosoxidans on contact lens",
pages = "300-293",
number = "3",
volume = "64",
doi = "10.1556/030.64.2017.005"
}

Konstantinović, N. M., Ćirković, I., Đukić, S., Marić, V.,& Božić, D. D.. (2017). Biofilm formation of achromobacter xylosoxidans on contact lens. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 64(3), 293-300.
https://doi.org/10.1556/030.64.2017.005

Konstantinović NM, Ćirković I, Đukić S, Marić V, Božić DD. Biofilm formation of achromobacter xylosoxidans on contact lens. in Acta Microbiologica et Immunologica Hungarica. 2017;64(3):293-300.
doi:10.1556/030.64.2017.005 .

Konstantinović, Neda M., Ćirković, Ivana, Đukić, Slobodanka, Marić, Vesna, Božić, Dragana D., "Biofilm formation of achromobacter xylosoxidans on contact lens" in Acta Microbiologica et Immunologica Hungarica, 64, no. 3 (2017):293-300,
https://doi.org/10.1556/030.64.2017.005 . .

TY  - JOUR
AU  - Pavlović, Bojan
AU  - Božić, Dragana D.
AU  - Milovanović, Jovica
AU  - Jotić, Ana
AU  - Đukić, Vojko
AU  - Đukić, Slobodanka
AU  - Konstantinović, Neda M.
AU  - Ćirković, Ivana
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/695
AB  - Objectives: Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints. Methods: Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations. Results: All tested strains formed different amounts of biofilm on SS: 66.7% S. aureus and 93.3% M. catarrhalis were weak biofilm producers and 33.3% S. aureus and 6.7% M. catarrhalis were moderate biofilm producers. S. aureus formed significantly higher quantity of biofilm compared with M. catarrhalis (p  lt  0.05). Multidrug resistant S. aureus produced significantly higher amount of biofilm compared with non-multidrug resistant strains (p  lt  0.05). Conclusion: Quantity of biofilm on SS is highly dependent on bacterial species and their resistance patterns. Future studies are needed to ascertain another therapeutic option for prophylaxis prior to SS placement.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Microbiologica et Immunologica Hungarica
T1  - Quantification of biofilm formation on silicone intranasal splints: an in vitro study
EP  - 311
IS  - 3
SP  - 301
VL  - 63
DO  - 10.1556/030.63.2016.006
ER  - 

@article{
author = "Pavlović, Bojan and Božić, Dragana D. and Milovanović, Jovica and Jotić, Ana and Đukić, Vojko and Đukić, Slobodanka and Konstantinović, Neda M. and Ćirković, Ivana",
year = "2016",
abstract = "Objectives: Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints. Methods: Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations. Results: All tested strains formed different amounts of biofilm on SS: 66.7% S. aureus and 93.3% M. catarrhalis were weak biofilm producers and 33.3% S. aureus and 6.7% M. catarrhalis were moderate biofilm producers. S. aureus formed significantly higher quantity of biofilm compared with M. catarrhalis (p  lt  0.05). Multidrug resistant S. aureus produced significantly higher amount of biofilm compared with non-multidrug resistant strains (p  lt  0.05). Conclusion: Quantity of biofilm on SS is highly dependent on bacterial species and their resistance patterns. Future studies are needed to ascertain another therapeutic option for prophylaxis prior to SS placement.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Microbiologica et Immunologica Hungarica",
title = "Quantification of biofilm formation on silicone intranasal splints: an in vitro study",
pages = "311-301",
number = "3",
volume = "63",
doi = "10.1556/030.63.2016.006"
}

Pavlović, B., Božić, D. D., Milovanović, J., Jotić, A., Đukić, V., Đukić, S., Konstantinović, N. M.,& Ćirković, I.. (2016). Quantification of biofilm formation on silicone intranasal splints: an in vitro study. in Acta Microbiologica et Immunologica Hungarica
Akademiai Kiado Rt, Budapest., 63(3), 301-311.
https://doi.org/10.1556/030.63.2016.006

Pavlović B, Božić DD, Milovanović J, Jotić A, Đukić V, Đukić S, Konstantinović NM, Ćirković I. Quantification of biofilm formation on silicone intranasal splints: an in vitro study. in Acta Microbiologica et Immunologica Hungarica. 2016;63(3):301-311.
doi:10.1556/030.63.2016.006 .

Pavlović, Bojan, Božić, Dragana D., Milovanović, Jovica, Jotić, Ana, Đukić, Vojko, Đukić, Slobodanka, Konstantinović, Neda M., Ćirković, Ivana, "Quantification of biofilm formation on silicone intranasal splints: an in vitro study" in Acta Microbiologica et Immunologica Hungarica, 63, no. 3 (2016):301-311,
https://doi.org/10.1556/030.63.2016.006 . .

TY  - JOUR
AU  - Ćirković, Ivana
AU  - Pavlović, Ljiljana
AU  - Konstantinović, Neda M.
AU  - Kostić, Katarina
AU  - Jovanović, Snežana
AU  - Đukić, Slobodanka
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/546
AB  - Introduction Beta-lactam antibiotics are the most commonly used antibacterial drugs. However, many bacteria have developed resistance to these antibiotics, and the most common form of resistance. is the production of beta-lactamase enzymes. Many members of the Enterobacteriaceae family produce different types of these enzymes. Objective The aim of this study was to perform phenotypic detection of production and identification of beta-lactamase type in Enterobacteriaceae isolated from different clinical specimens from patients hospitalized in the Clinical Center of Serbia. Methods The strains of Enterobacteriaceae were collected between November 2011 and January 2012 in the laboratory of the Clinical Center of Serbia. The isolates were identified according to the standard microbiology procedures and confirmed by the Vitek2 automated system. Antimicrobial susceptibility testing was performed by the disk diffusion method, and the phenotypic detection of production and identification of beta-lactamases was performed according to previously described methodologies. Results In this study, a total of 172 Enterobacteriaceae strains were isolated. Further testing was performed on 54/145 (37.2%) strains showing decreased susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli, 31/46 (67.4%) Klebsiella pneumoniae and 10/14(71.4%) Proteus mirabilis. Among them, 40/145 (27.6%) strains produced extended spectrum beta-lactamases (ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145 (2.8%) - carbapenemases. Carbapenemases were predominantly detected in K. pneumoniae (75%). Conclusion Enterobacteriaceae produce different types of beta-lactamases, and the most common type in our study was ESBLs. Production of carbapenemases detected in Enterobacteriaceae is also an associated problem.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae
EP  - 463
IS  - 7-8
SP  - 457
VL  - 142
DO  - 10.2298/SARH1408457C
ER  - 

@article{
author = "Ćirković, Ivana and Pavlović, Ljiljana and Konstantinović, Neda M. and Kostić, Katarina and Jovanović, Snežana and Đukić, Slobodanka",
year = "2014",
abstract = "Introduction Beta-lactam antibiotics are the most commonly used antibacterial drugs. However, many bacteria have developed resistance to these antibiotics, and the most common form of resistance. is the production of beta-lactamase enzymes. Many members of the Enterobacteriaceae family produce different types of these enzymes. Objective The aim of this study was to perform phenotypic detection of production and identification of beta-lactamase type in Enterobacteriaceae isolated from different clinical specimens from patients hospitalized in the Clinical Center of Serbia. Methods The strains of Enterobacteriaceae were collected between November 2011 and January 2012 in the laboratory of the Clinical Center of Serbia. The isolates were identified according to the standard microbiology procedures and confirmed by the Vitek2 automated system. Antimicrobial susceptibility testing was performed by the disk diffusion method, and the phenotypic detection of production and identification of beta-lactamases was performed according to previously described methodologies. Results In this study, a total of 172 Enterobacteriaceae strains were isolated. Further testing was performed on 54/145 (37.2%) strains showing decreased susceptibility to beta-lactam antibiotics: 13/85 (15.3%) Escherichia coli, 31/46 (67.4%) Klebsiella pneumoniae and 10/14(71.4%) Proteus mirabilis. Among them, 40/145 (27.6%) strains produced extended spectrum beta-lactamases (ESBLs), 9/145 (6.2%) - AmpC, 1/145 (0.7%) - K1 beta-lactamase and 4/145 (2.8%) - carbapenemases. Carbapenemases were predominantly detected in K. pneumoniae (75%). Conclusion Enterobacteriaceae produce different types of beta-lactamases, and the most common type in our study was ESBLs. Production of carbapenemases detected in Enterobacteriaceae is also an associated problem.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae",
pages = "463-457",
number = "7-8",
volume = "142",
doi = "10.2298/SARH1408457C"
}

Ćirković, I., Pavlović, L., Konstantinović, N. M., Kostić, K., Jovanović, S.,& Đukić, S.. (2014). Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(7-8), 457-463.
https://doi.org/10.2298/SARH1408457C

Ćirković I, Pavlović L, Konstantinović NM, Kostić K, Jovanović S, Đukić S. Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae. in Srpski arhiv za celokupno lekarstvo. 2014;142(7-8):457-463.
doi:10.2298/SARH1408457C .

Ćirković, Ivana, Pavlović, Ljiljana, Konstantinović, Neda M., Kostić, Katarina, Jovanović, Snežana, Đukić, Slobodanka, "Phenotypic Detection of Beta-Lactamases Production in Enterobacteriaceae" in Srpski arhiv za celokupno lekarstvo, 142, no. 7-8 (2014):457-463,
https://doi.org/10.2298/SARH1408457C . .