TY  - JOUR
AU  - Zelenović, Nevena
AU  - Kojadinović, Milica
AU  - Filipović, Lidija
AU  - Vučić, Vesna
AU  - Milčić, Miloš
AU  - Arsić, Aleksandra
AU  - Popović, Milica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1309
AB  - Backgound/Objectives: Urolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest. Methods: A powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein. Results: The spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236 ± 5706 and 69653 ± 14922, respectively, while for UROAG and UROBG, these values were 15179 ± 2770 and 9462 ± 1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site. Conclusion: The aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.
PB  - SAGE
T2  - Natural Product Communications
T1  - Interactions of Different Urolithins With Bovine Serum Albumin
IS  - 5
VL  - 18
DO  - 10.1177/1934578X231169366
ER  - 

@article{
author = "Zelenović, Nevena and Kojadinović, Milica and Filipović, Lidija and Vučić, Vesna and Milčić, Miloš and Arsić, Aleksandra and Popović, Milica",
year = "2023",
abstract = "Backgound/Objectives: Urolithins (UROs) are the metabolites derived from the gut microbial action on ellagitannins and ellagic acid-rich foods. Following their absorption in the intestine, UROs are transported through the systemic circulation to various tissues where they can express their biological function as antimicrobial, anti-inflammatory, and anticancer agents. In addition to blood plasma, where they can be found as glucuronide and sulfate conjugates, they are also found in urine. Therefore, the interactions of UROs with serum proteins are of great clinical interest. Methods: A powerful technique for examining these urolithin-serum protein interactions is fluorescence spectroscopy. Bovine serum albumin (BSA) is a particularly suitable model protein because it is readily available, affordable, and similar to human serum albumin. This work aimed to study the binding of UROs (urolithin A, UROA and urolithin B, UROB) and their glucuronide conjugates (UROAG and UROBG) to BSA by quenching the intrinsic fluorescence of protein. Results: The spectra obtained showed that the binding process is influenced by the polyphenol's structure and the conjugation process with the glucuronide. The calculated Stern Vollmer binding constants (Ksv): UROA and UROB Ksv were 59236 ± 5706 and 69653 ± 14922, respectively, while for UROAG and UROBG, these values were 15179 ± 2770 and 9462 ± 1955, respectively, which showed that the binding affinity decreased with glucuronidation. Molecular docking studies confirmed that all of the studied molecules will bind favorably to BSA. The preferential binding site for both UROs and UROGs is Sudlow I, while UROs will also bind to Sudlow II. URO-Gs can bind to BSA in the cleft region with lower binding scores than for the Sudlow I binding site. Conclusion: The aglycone's higher hydrophobicity increases the binding affinity to BSA, thus reducing its bioavailability in the blood.",
publisher = "SAGE",
journal = "Natural Product Communications",
title = "Interactions of Different Urolithins With Bovine Serum Albumin",
number = "5",
volume = "18",
doi = "10.1177/1934578X231169366"
}

Zelenović, N., Kojadinović, M., Filipović, L., Vučić, V., Milčić, M., Arsić, A.,& Popović, M.. (2023). Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications
SAGE., 18(5).
https://doi.org/10.1177/1934578X231169366

Zelenović N, Kojadinović M, Filipović L, Vučić V, Milčić M, Arsić A, Popović M. Interactions of Different Urolithins With Bovine Serum Albumin. in Natural Product Communications. 2023;18(5).
doi:10.1177/1934578X231169366 .

Zelenović, Nevena, Kojadinović, Milica, Filipović, Lidija, Vučić, Vesna, Milčić, Miloš, Arsić, Aleksandra, Popović, Milica, "Interactions of Different Urolithins With Bovine Serum Albumin" in Natural Product Communications, 18, no. 5 (2023),
https://doi.org/10.1177/1934578X231169366 . .

TY  - JOUR
AU  - Filipović, Lidija
AU  - Kojadinović, Milica
AU  - Popović, Milica
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1204
AB  - Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?
SP  - 103057
VL  - 68
DO  - 10.1016/j.jddst.2021.103057
ER  - 

@article{
author = "Filipović, Lidija and Kojadinović, Milica and Popović, Milica",
year = "2022",
abstract = "Exosomes are a sub-group of extracellular vesicles, playing an important part in a cell-cell communication in many physiological and pathological conditions. Their size and competence for transferring material to recipient cells make them a promising nanocarrier for clinical use. Their non-immunogenic nature, similar to the body's own structure make them far superior transporters compared to liposomes and polymeric nanoparticles. This review, will provide an overview of exosome biogenesis, biological role, and purification methods. The focus of this manuscript will be to summarize specific applications of exosomes and exosome-mimetics as drug delivery systems in pharmaceutical drug development. We will describe drug-loading approaches, in vivo and in vitro exosome tracing methods, specific modifications and examples of the delivery of therapeutic and imaging molecules from a variety of biological origins. Challenges in the translation of exosome-based drug carriers to clinical use will also be discussed in this review.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?",
pages = "103057",
volume = "68",
doi = "10.1016/j.jddst.2021.103057"
}

Filipović, L., Kojadinović, M.,& Popović, M.. (2022). Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?. in Journal of Drug Delivery Science and Technology
Elsevier., 68, 103057.
https://doi.org/10.1016/j.jddst.2021.103057

Filipović L, Kojadinović M, Popović M. Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?. in Journal of Drug Delivery Science and Technology. 2022;68:103057.
doi:10.1016/j.jddst.2021.103057 .

Filipović, Lidija, Kojadinović, Milica, Popović, Milica, "Exosomes and exosome-mimetics as targeted drug carriers: Where we stand and what the future holds?" in Journal of Drug Delivery Science and Technology, 68 (2022):103057,
https://doi.org/10.1016/j.jddst.2021.103057 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Ristić-Medić, Danijela
AU  - Krstić, Đurđa
AU  - Rovčanin, Branislav
AU  - Rađen, Slavica
AU  - Terzić, Brankica
AU  - Manojlović, Dragan
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1221
AB  - The status of essential and toxic trace elements in patients with different stages of chronic kidney disease (CKD) is still unclear and not well characterized. The present study examined the circulatory levels of a wide panel of trace elements (Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Cd, Pb, and U) in hemodialysis patients (HD group) and pre-dialysis patients with stage 3 CKD (PD group). Comparisons were made between groups of patients and healthy individuals from the control group (CG). The levels of Al, Mn, Co, Ni, Cu, As, Se, Sr, and Pb were higher, while the levels of Cr, Zn, Rb, Cd, and U were lower in HD patients than in our CG. Higher levels of Al and Se, as well as lower levels of As, Sr, Zn, Rb, and U were significant and distinguished HD from PD. Among other analyzed elements, Co, Se, and U are the only trace elements that did not distinguish PD from CG at a statistically significant level. The HD group had lower serum U levels than the PD group, and this could be a result of hemodialysis. This study also revealed that the Cu/Zn ratio could be used as a marker for early and late detection of renal failure. Marked changes of essential and toxic trace element levels in sera indicate additional pathophysiological events in CKD, which could additionally contribute to the preexisting increased morbidity of HD patients. Measurement of trace elements in HD patients should be performed routinely.
PB  - Wiley-Blackwell
T2  - Biological Trace Element Research
T1  - Circulatory Imbalance of Essential and Toxic Trace Elements in Pre-dialysis and Hemodialysis Patients
EP  - 3125
IS  - 7
SP  - 3117
VL  - 200
DO  - 10.1007/s12011-021-02940-7
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Ristić-Medić, Danijela and Krstić, Đurđa and Rovčanin, Branislav and Rađen, Slavica and Terzić, Brankica and Manojlović, Dragan",
year = "2022",
abstract = "The status of essential and toxic trace elements in patients with different stages of chronic kidney disease (CKD) is still unclear and not well characterized. The present study examined the circulatory levels of a wide panel of trace elements (Al, Cr, Mn, Co, Ni, Cu, Zn, As, Se, Rb, Sr, Cd, Pb, and U) in hemodialysis patients (HD group) and pre-dialysis patients with stage 3 CKD (PD group). Comparisons were made between groups of patients and healthy individuals from the control group (CG). The levels of Al, Mn, Co, Ni, Cu, As, Se, Sr, and Pb were higher, while the levels of Cr, Zn, Rb, Cd, and U were lower in HD patients than in our CG. Higher levels of Al and Se, as well as lower levels of As, Sr, Zn, Rb, and U were significant and distinguished HD from PD. Among other analyzed elements, Co, Se, and U are the only trace elements that did not distinguish PD from CG at a statistically significant level. The HD group had lower serum U levels than the PD group, and this could be a result of hemodialysis. This study also revealed that the Cu/Zn ratio could be used as a marker for early and late detection of renal failure. Marked changes of essential and toxic trace element levels in sera indicate additional pathophysiological events in CKD, which could additionally contribute to the preexisting increased morbidity of HD patients. Measurement of trace elements in HD patients should be performed routinely.",
publisher = "Wiley-Blackwell",
journal = "Biological Trace Element Research",
title = "Circulatory Imbalance of Essential and Toxic Trace Elements in Pre-dialysis and Hemodialysis Patients",
pages = "3125-3117",
number = "7",
volume = "200",
doi = "10.1007/s12011-021-02940-7"
}

Stojsavljević, A., Ristić-Medić, D., Krstić, Đ., Rovčanin, B., Rađen, S., Terzić, B.,& Manojlović, D.. (2022). Circulatory Imbalance of Essential and Toxic Trace Elements in Pre-dialysis and Hemodialysis Patients. in Biological Trace Element Research
Wiley-Blackwell., 200(7), 3117-3125.
https://doi.org/10.1007/s12011-021-02940-7

Stojsavljević A, Ristić-Medić D, Krstić Đ, Rovčanin B, Rađen S, Terzić B, Manojlović D. Circulatory Imbalance of Essential and Toxic Trace Elements in Pre-dialysis and Hemodialysis Patients. in Biological Trace Element Research. 2022;200(7):3117-3125.
doi:10.1007/s12011-021-02940-7 .

Stojsavljević, Aleksandar, Ristić-Medić, Danijela, Krstić, Đurđa, Rovčanin, Branislav, Rađen, Slavica, Terzić, Brankica, Manojlović, Dragan, "Circulatory Imbalance of Essential and Toxic Trace Elements in Pre-dialysis and Hemodialysis Patients" in Biological Trace Element Research, 200, no. 7 (2022):3117-3125,
https://doi.org/10.1007/s12011-021-02940-7 . .

Opsenica, I., Selaković, M., Tot, M., Verbić, T., Srbljanović, J., Štajner, T., Đurković-Đaković, O.,& Šolaja, B.. (2021). New 4-aminoquinolines as moderate inhibitors of P. falciparum malaria. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 86(2), 115-123.
https://doi.org/10.2298/JSC201225005O

Opsenica I, Selaković M, Tot M, Verbić T, Srbljanović J, Štajner T, Đurković-Đaković O, Šolaja B. New 4-aminoquinolines as moderate inhibitors of P. falciparum malaria. in Journal of the Serbian Chemical Society. 2021;86(2):115-123.
doi:10.2298/JSC201225005O .

Opsenica, Igor, Selaković, Milica, Tot, Mikloš, Verbić, Tatjana, Srbljanović, Jelena, Štajner, Tijana, Đurković-Đaković, Olgica, Šolaja, Bogdan, "New 4-aminoquinolines as moderate inhibitors of P. falciparum malaria" in Journal of the Serbian Chemical Society, 86, no. 2 (2021):115-123,
https://doi.org/10.2298/JSC201225005O . .