Detection and genotyping of Toxoplasma gondii in wild canids in Serbia
Само за регистроване кориснике
2019
Аутори
Uzelac, AleksandraKlun, Ivana
Ćirović, Duško
Penezić, Aleksandra
Ćirković, Vladimir
Đurković-Đaković, Olgica
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in ...golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains.
Кључне речи:
Toxoplasma gondii / Genotyping / Strains / Golden jackal / Red fox / Grey wolfИзвор:
Parasitology International, 2019, 73Издавач:
- Elsevier Ireland Ltd, Clare
Финансирање / пројекти:
- Контрола инфекција апикомплексним патогенима: од нових места деловања лека до предикције (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41019)
- Савремени концепти газдовања популацијама дивљачи у циљу веће економске валоризације (RS-MESTD-Technological Development (TD or TR)-31009)
DOI: 10.1016/j.parint.2019.101973
ISSN: 1383-5769
PubMed: 31404655
WoS: 000487571300006
Scopus: 2-s2.0-85070808415
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Uzelac, Aleksandra AU - Klun, Ivana AU - Ćirović, Duško AU - Penezić, Aleksandra AU - Ćirković, Vladimir AU - Đurković-Đaković, Olgica PY - 2019 UR - http://rimi.imi.bg.ac.rs/handle/123456789/906 AB - To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains. PB - Elsevier Ireland Ltd, Clare T2 - Parasitology International T1 - Detection and genotyping of Toxoplasma gondii in wild canids in Serbia VL - 73 DO - 10.1016/j.parint.2019.101973 ER -
@article{ author = "Uzelac, Aleksandra and Klun, Ivana and Ćirović, Duško and Penezić, Aleksandra and Ćirković, Vladimir and Đurković-Đaković, Olgica", year = "2019", abstract = "To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains.", publisher = "Elsevier Ireland Ltd, Clare", journal = "Parasitology International", title = "Detection and genotyping of Toxoplasma gondii in wild canids in Serbia", volume = "73", doi = "10.1016/j.parint.2019.101973" }
Uzelac, A., Klun, I., Ćirović, D., Penezić, A., Ćirković, V.,& Đurković-Đaković, O.. (2019). Detection and genotyping of Toxoplasma gondii in wild canids in Serbia. in Parasitology International Elsevier Ireland Ltd, Clare., 73. https://doi.org/10.1016/j.parint.2019.101973
Uzelac A, Klun I, Ćirović D, Penezić A, Ćirković V, Đurković-Đaković O. Detection and genotyping of Toxoplasma gondii in wild canids in Serbia. in Parasitology International. 2019;73. doi:10.1016/j.parint.2019.101973 .
Uzelac, Aleksandra, Klun, Ivana, Ćirović, Duško, Penezić, Aleksandra, Ćirković, Vladimir, Đurković-Đaković, Olgica, "Detection and genotyping of Toxoplasma gondii in wild canids in Serbia" in Parasitology International, 73 (2019), https://doi.org/10.1016/j.parint.2019.101973 . .