TY  - JOUR
AU  - Nedisan, Maria E.
AU  - Gyorke, Adriana
AU  - Stefanut, Cristina L.
AU  - Kalmar, Zsuzsa
AU  - Friss, Zsuzsa
AU  - Blaga, Radu
AU  - Blaizot, Amandine
AU  - Toma-Naić, Andra
AU  - Mircean, Viorica
AU  - Schares, Gereon
AU  - Đurković-Đaković, Olgica
AU  - Klun, Ivana
AU  - Villena, Isabelle
AU  - Cozma, Vasile
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1078
AB  - Toxoplasma gondii is a widespread zoonotic protozoan that infects most species of mammals and birds, including poultry. This study aimed to investigate the course of T. gondii infection and the efficacy of diclazuril and Artemisia annua in preventing infection in experimentally infected chickens. Seventy-five 1-month-old chickens, female and male, were randomly divided into five groups (n = 15 each) as follows: (1) uninfected untreated (negative control, NC); (2) infected with T. gondii genotype II/III isolated from a wild cat (group WC); (3) infected with T. gondii genotype II isolated from a domestic cat (group DC); (4) infected with T. gondii domestic cat strain and treated with the anticoccidial diclazuril (group DC-D); and (5) infected with T. gondii domestic cat strain and treated with the medicinal plant Artemisia annua (group DC-A). Clinical signs, body temperature, mortality rate, weight gain, feed conversion ratio, hematological parameters, and the presence of T. gondii-specific IgY antibodies were recorded in all groups. Five chickens per group were euthanized 28 days post-infection (p.i.) and their brains, hearts, and breast muscle tested for T. gondii by mouse bioassay and polymerase chain reaction (PCR). No clinical signs related to the experimental infection were observed throughout the study period. T. gondii-specific antibodies were detected by day 28 p.i., but not in all infected chickens. Overall, T. gondii DNA was detected (bioassay or tissue digests) in all infected and untreated chickens (10/10), while viable parasite (bioassay) was isolated from 7 out of 10 chickens. The parasite was most frequently identified in the brain (7/10). There were no differences in the T. gondii strains regarding clinical infection and the rate of T. gondii detection in tissues. However, higher antibody titers were obtained in chickens infected with T. gondii WC strain (1:192) comparing with T. gondii DC strain (1:48). A. annua reduced replication of the parasite in 3 out of 5 chickens, while diclazuril did not. In conclusion, broiler chickens were resistant to clinical toxoplasmosis, irrespective of the strain (domestic or wild cat strain). The herb A. annua presented prophylactic efficacy by reduced parasite replication. However, further studies are required aiming at the efficacy of diclazuril and A. annua for the prevention of T. gondii infection in chickens using quantitative analysis methods.
PB  - Springer, New York
T2  - Parasitology Research
T1  - Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis
EP  - 603
IS  - 2
SP  - 593
VL  - 120
DO  - 10.1007/s00436-020-06984-x
ER  - 

@article{
author = "Nedisan, Maria E. and Gyorke, Adriana and Stefanut, Cristina L. and Kalmar, Zsuzsa and Friss, Zsuzsa and Blaga, Radu and Blaizot, Amandine and Toma-Naić, Andra and Mircean, Viorica and Schares, Gereon and Đurković-Đaković, Olgica and Klun, Ivana and Villena, Isabelle and Cozma, Vasile",
year = "2021",
abstract = "Toxoplasma gondii is a widespread zoonotic protozoan that infects most species of mammals and birds, including poultry. This study aimed to investigate the course of T. gondii infection and the efficacy of diclazuril and Artemisia annua in preventing infection in experimentally infected chickens. Seventy-five 1-month-old chickens, female and male, were randomly divided into five groups (n = 15 each) as follows: (1) uninfected untreated (negative control, NC); (2) infected with T. gondii genotype II/III isolated from a wild cat (group WC); (3) infected with T. gondii genotype II isolated from a domestic cat (group DC); (4) infected with T. gondii domestic cat strain and treated with the anticoccidial diclazuril (group DC-D); and (5) infected with T. gondii domestic cat strain and treated with the medicinal plant Artemisia annua (group DC-A). Clinical signs, body temperature, mortality rate, weight gain, feed conversion ratio, hematological parameters, and the presence of T. gondii-specific IgY antibodies were recorded in all groups. Five chickens per group were euthanized 28 days post-infection (p.i.) and their brains, hearts, and breast muscle tested for T. gondii by mouse bioassay and polymerase chain reaction (PCR). No clinical signs related to the experimental infection were observed throughout the study period. T. gondii-specific antibodies were detected by day 28 p.i., but not in all infected chickens. Overall, T. gondii DNA was detected (bioassay or tissue digests) in all infected and untreated chickens (10/10), while viable parasite (bioassay) was isolated from 7 out of 10 chickens. The parasite was most frequently identified in the brain (7/10). There were no differences in the T. gondii strains regarding clinical infection and the rate of T. gondii detection in tissues. However, higher antibody titers were obtained in chickens infected with T. gondii WC strain (1:192) comparing with T. gondii DC strain (1:48). A. annua reduced replication of the parasite in 3 out of 5 chickens, while diclazuril did not. In conclusion, broiler chickens were resistant to clinical toxoplasmosis, irrespective of the strain (domestic or wild cat strain). The herb A. annua presented prophylactic efficacy by reduced parasite replication. However, further studies are required aiming at the efficacy of diclazuril and A. annua for the prevention of T. gondii infection in chickens using quantitative analysis methods.",
publisher = "Springer, New York",
journal = "Parasitology Research",
title = "Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis",
pages = "603-593",
number = "2",
volume = "120",
doi = "10.1007/s00436-020-06984-x"
}

Nedisan, M. E., Gyorke, A., Stefanut, C. L., Kalmar, Z., Friss, Z., Blaga, R., Blaizot, A., Toma-Naić, A., Mircean, V., Schares, G., Đurković-Đaković, O., Klun, I., Villena, I.,& Cozma, V.. (2021). Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis. in Parasitology Research
Springer, New York., 120(2), 593-603.
https://doi.org/10.1007/s00436-020-06984-x

Nedisan ME, Gyorke A, Stefanut CL, Kalmar Z, Friss Z, Blaga R, Blaizot A, Toma-Naić A, Mircean V, Schares G, Đurković-Đaković O, Klun I, Villena I, Cozma V. Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis. in Parasitology Research. 2021;120(2):593-603.
doi:10.1007/s00436-020-06984-x .

Nedisan, Maria E., Gyorke, Adriana, Stefanut, Cristina L., Kalmar, Zsuzsa, Friss, Zsuzsa, Blaga, Radu, Blaizot, Amandine, Toma-Naić, Andra, Mircean, Viorica, Schares, Gereon, Đurković-Đaković, Olgica, Klun, Ivana, Villena, Isabelle, Cozma, Vasile, "Experimental infection with Toxoplasma gondii in broiler chickens (Gallus domesticus): seroconversion, tissue cyst distribution, and prophylaxis" in Parasitology Research, 120, no. 2 (2021):593-603,
https://doi.org/10.1007/s00436-020-06984-x . .

TY  - JOUR
AU  - Blaga, Radu
AU  - Aubert, Dominique
AU  - Thébault, A.
AU  - Perret, Catherine
AU  - Geers, R.
AU  - Thomas, M.
AU  - Alliot, A.
AU  - Đokić, Vitomir
AU  - Ortis, N.
AU  - Halos, L.
AU  - Durand, B.
AU  - Mercier, Aurelien
AU  - Villena, Isabelle
AU  - Boireau, Pascal
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/921
AB  - In France, the consumption of cattle and sheep meat appears to be a risk factor for infection of pregnant women with Toxoplasma gondii. Several nation-wide surveys in France have investigated the prevalence of T. gondii in sheep and pig meat, but little is known at present about the prevalence of the parasite in beef. The main objective of the present cross-sectional survey was to estimate the seroprevalence of T. gondii infection in beef consumed in France. A secondary objective was to attempt to isolate T. gondii from cattle tissues and to study the geographical and age variations of this seroprevalence. The overall estimate of seroprevalence of T. gondii in bovine carcasses (n = 2912), for a threshold of 1:6 was 17.38%. A strong age effect was observed (p  lt  0.0001) with a seroprevalence of 5.34% for calves ( lt 8 months) and 23.12% for adults ( gt 8 months). Seroprevalence estimates given by area of birth and area of slaughtering for adults showed that the areas with the highest seroprevalence were not the same between these two variables. Only two strains, corresponding to genotype II, were isolated from heart samples, indicating that there is a limited risk of human infection with T. gondii, which needs to be correlated with the food habit of consuming raw or undercook (bleu or saignant) beef. However, new questions have emerged, especially concerning the isolation of parasites from beef and the precise role of bovines, generally described as poor hosts for T. gondii, in human infection.
PB  - EDP Sciences
T2  - Parasite
T1  - Toxoplasma gondii in beef consumed in France: Regional variation in seroprevalence and parasite isolation
VL  - 26
DO  - 10.1051/parasite/2019076
ER  - 

@article{
author = "Blaga, Radu and Aubert, Dominique and Thébault, A. and Perret, Catherine and Geers, R. and Thomas, M. and Alliot, A. and Đokić, Vitomir and Ortis, N. and Halos, L. and Durand, B. and Mercier, Aurelien and Villena, Isabelle and Boireau, Pascal",
year = "2019",
abstract = "In France, the consumption of cattle and sheep meat appears to be a risk factor for infection of pregnant women with Toxoplasma gondii. Several nation-wide surveys in France have investigated the prevalence of T. gondii in sheep and pig meat, but little is known at present about the prevalence of the parasite in beef. The main objective of the present cross-sectional survey was to estimate the seroprevalence of T. gondii infection in beef consumed in France. A secondary objective was to attempt to isolate T. gondii from cattle tissues and to study the geographical and age variations of this seroprevalence. The overall estimate of seroprevalence of T. gondii in bovine carcasses (n = 2912), for a threshold of 1:6 was 17.38%. A strong age effect was observed (p  lt  0.0001) with a seroprevalence of 5.34% for calves ( lt 8 months) and 23.12% for adults ( gt 8 months). Seroprevalence estimates given by area of birth and area of slaughtering for adults showed that the areas with the highest seroprevalence were not the same between these two variables. Only two strains, corresponding to genotype II, were isolated from heart samples, indicating that there is a limited risk of human infection with T. gondii, which needs to be correlated with the food habit of consuming raw or undercook (bleu or saignant) beef. However, new questions have emerged, especially concerning the isolation of parasites from beef and the precise role of bovines, generally described as poor hosts for T. gondii, in human infection.",
publisher = "EDP Sciences",
journal = "Parasite",
title = "Toxoplasma gondii in beef consumed in France: Regional variation in seroprevalence and parasite isolation",
volume = "26",
doi = "10.1051/parasite/2019076"
}

Blaga, R., Aubert, D., Thébault, A., Perret, C., Geers, R., Thomas, M., Alliot, A., Đokić, V., Ortis, N., Halos, L., Durand, B., Mercier, A., Villena, I.,& Boireau, P.. (2019). Toxoplasma gondii in beef consumed in France: Regional variation in seroprevalence and parasite isolation. in Parasite
EDP Sciences., 26.
https://doi.org/10.1051/parasite/2019076

Blaga R, Aubert D, Thébault A, Perret C, Geers R, Thomas M, Alliot A, Đokić V, Ortis N, Halos L, Durand B, Mercier A, Villena I, Boireau P. Toxoplasma gondii in beef consumed in France: Regional variation in seroprevalence and parasite isolation. in Parasite. 2019;26.
doi:10.1051/parasite/2019076 .

Blaga, Radu, Aubert, Dominique, Thébault, A., Perret, Catherine, Geers, R., Thomas, M., Alliot, A., Đokić, Vitomir, Ortis, N., Halos, L., Durand, B., Mercier, Aurelien, Villena, Isabelle, Boireau, Pascal, "Toxoplasma gondii in beef consumed in France: Regional variation in seroprevalence and parasite isolation" in Parasite, 26 (2019),
https://doi.org/10.1051/parasite/2019076 . .

TY  - JOUR
AU  - Klun, Ivana
AU  - Uzelac, Aleksandra
AU  - Villena, Isabelle
AU  - Mercier, Aurelien
AU  - Bobić, Branko
AU  - Nikolić, Aleksandra
AU  - Rajnpreht, Irena
AU  - Opsteegh, Marieke
AU  - Aubert, Dominique
AU  - Blaga, Radu
AU  - van der Giessen, Joke
AU  - Đurković-Đaković, Olgica
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/829
AB  - Background: Consumption of undercooked or insufficiently cured meat is a major risk factor for human infection with Toxoplasma gondii. Although horsemeat is typically consumed rare or undercooked, information on the risk of T. gondii from infected horse meat to humans is scarce. Here, we present the results of a study to determine the presence of T. gondii infection in slaughter horses in Serbia, and to attempt to isolate viable parasites. Methods: The study included horses from all regions of Serbia slaughtered at two abattoirs between June 2013 and June 2015. Blood sera were tested for the presence of specific IgG T. gondii antibodies by the modified agglutination test (MAT), and samples of trypsin-digested heart tissue were bioassayed in mice. Cyst-positive mouse brain homogenates were subjected to DNA extraction and T. gondii strains were genotyped using 15 microsatellite markers (MS). Results: A total of 105 slaughter horses were sampled. At the 1: 6 cut-off 48.6% of the examined horses were seropositive, with the highest titre being 1: 400. Viable parasites were isolated from two grade type mares; both parasite isolates (RS-Eq39 and RS-Eq40) were T. gondii type III, and both displayed an increased lethality for mice with successive passages. These are the first cases of isolation of T. gondii from horses in Serbia. When compared with a worldwide collection of 61 type III and type III-like strains, isolate RS-Eq39 showed a combination of MS lengths similar to a strain isolated from a duck in Iran, and isolate RS-Eq40 was identical in all markers to three strains isolated from a goat from Gabon, a sheep from France and a pig from Portugal. Interestingly, the source horses were one seronegative and one weakly seropositive. Conclusions: The isolation of viable T. gondii parasites from slaughter horses points to horsemeat as a potential source of human infection, but the fact that viable parasites were isolated from horses with only a serological trace of T. gondii infection presents further evidence that serology may not be adequate to assess the risk of toxoplasmosis from horsemeat consumption. Presence of T. gondii type III in Serbia sheds more light into the potential origin of this archetypal lineage in Europe.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia
IS  - 1
SP  - 167
VL  - 10
DO  - 10.1186/s13071-017-2104-x
ER  - 

@article{
author = "Klun, Ivana and Uzelac, Aleksandra and Villena, Isabelle and Mercier, Aurelien and Bobić, Branko and Nikolić, Aleksandra and Rajnpreht, Irena and Opsteegh, Marieke and Aubert, Dominique and Blaga, Radu and van der Giessen, Joke and Đurković-Đaković, Olgica",
year = "2017",
abstract = "Background: Consumption of undercooked or insufficiently cured meat is a major risk factor for human infection with Toxoplasma gondii. Although horsemeat is typically consumed rare or undercooked, information on the risk of T. gondii from infected horse meat to humans is scarce. Here, we present the results of a study to determine the presence of T. gondii infection in slaughter horses in Serbia, and to attempt to isolate viable parasites. Methods: The study included horses from all regions of Serbia slaughtered at two abattoirs between June 2013 and June 2015. Blood sera were tested for the presence of specific IgG T. gondii antibodies by the modified agglutination test (MAT), and samples of trypsin-digested heart tissue were bioassayed in mice. Cyst-positive mouse brain homogenates were subjected to DNA extraction and T. gondii strains were genotyped using 15 microsatellite markers (MS). Results: A total of 105 slaughter horses were sampled. At the 1: 6 cut-off 48.6% of the examined horses were seropositive, with the highest titre being 1: 400. Viable parasites were isolated from two grade type mares; both parasite isolates (RS-Eq39 and RS-Eq40) were T. gondii type III, and both displayed an increased lethality for mice with successive passages. These are the first cases of isolation of T. gondii from horses in Serbia. When compared with a worldwide collection of 61 type III and type III-like strains, isolate RS-Eq39 showed a combination of MS lengths similar to a strain isolated from a duck in Iran, and isolate RS-Eq40 was identical in all markers to three strains isolated from a goat from Gabon, a sheep from France and a pig from Portugal. Interestingly, the source horses were one seronegative and one weakly seropositive. Conclusions: The isolation of viable T. gondii parasites from slaughter horses points to horsemeat as a potential source of human infection, but the fact that viable parasites were isolated from horses with only a serological trace of T. gondii infection presents further evidence that serology may not be adequate to assess the risk of toxoplasmosis from horsemeat consumption. Presence of T. gondii type III in Serbia sheds more light into the potential origin of this archetypal lineage in Europe.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia",
number = "1",
pages = "167",
volume = "10",
doi = "10.1186/s13071-017-2104-x"
}

Klun, I., Uzelac, A., Villena, I., Mercier, A., Bobić, B., Nikolić, A., Rajnpreht, I., Opsteegh, M., Aubert, D., Blaga, R., van der Giessen, J.,& Đurković-Đaković, O.. (2017). The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia. in Parasites & Vectors
BMC, London., 10(1), 167.
https://doi.org/10.1186/s13071-017-2104-x

Klun I, Uzelac A, Villena I, Mercier A, Bobić B, Nikolić A, Rajnpreht I, Opsteegh M, Aubert D, Blaga R, van der Giessen J, Đurković-Đaković O. The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia. in Parasites & Vectors. 2017;10(1):167.
doi:10.1186/s13071-017-2104-x .

Klun, Ivana, Uzelac, Aleksandra, Villena, Isabelle, Mercier, Aurelien, Bobić, Branko, Nikolić, Aleksandra, Rajnpreht, Irena, Opsteegh, Marieke, Aubert, Dominique, Blaga, Radu, van der Giessen, Joke, Đurković-Đaković, Olgica, "The first isolation and molecular characterization of Toxoplasma gondii from horses in Serbia" in Parasites & Vectors, 10, no. 1 (2017):167,
https://doi.org/10.1186/s13071-017-2104-x . .

TY  - JOUR
AU  - Đokić, Vitomir
AU  - Fablet, C.
AU  - Blaga, Radu
AU  - Rose, N.
AU  - Perret, Catherine
AU  - Đurković-Đaković, Olgica
AU  - Boireau, Pascal
AU  - Durand, B.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/737
AB  - Background: Infection by Toxoplasma gondii postnatally can occur after ingestion of contaminated meat or water (tissue cysts/oocysts). In Europe, percentage of meat borne infections is estimated between 30 and 63 %, out of which pork makes the most important source. The aim of this study was to (i) investigate the seroprevalence of T. gondii in intensive pig farms from western France; and (ii) identify the risk factors associated with seropositivity. Methods: Data were collected between November 2006 and February 2008 in 60 intensive farrow-to-finish farms, where sera were taken from 3595 fattening pigs, weaned and suckling piglets. Information about three classes of potential seropositivity risk factors were obtained through a questionnaire concerning: (i) breeding characteristics; (ii) farm management; and (iii) husbandry and hygiene. The modified agglutination test (MAT) was used for detection of specific anti T. gondii antibodies in pig sera, starting from 1/6 dilution. Results: The overall proportion of seropositive animals was 6.9 %, but the proportion of herds with at least one positive pig was 100 %. Multivariate logistic mixed model showed an increased seropositivity risk in weaned compared to suckling piglets, and a decreasing risk for mid-sized and large farms. The presence of a Danish entry facility, that clearly separates clean and dirty areas, had a protective effect on T. gondii seropositivity as well. Conclusions: The observed proportion of herds with at least one T. gondii seropositive animal provides further evidence that even in confined conditions of pig breeding, infection occurs, and is common. The highest risk for acquiring T. gondii is at the end of weaning period. Smaller confined pig farms demonstrate higher T. gondii seropositivity levels. This study also showed that Danish entry on farm buildings provides effective protection against T. gondii.
PB  - Biomed Central Ltd, London
T2  - Parasites & Vectors
T1  - Factors associated with Toxoplasma gondii infection in confined farrow-to-finish pig herds in western France: an exploratory study in 60 herds
VL  - 9
DO  - 10.1186/s13071-016-1753-5
ER  - 

@article{
author = "Đokić, Vitomir and Fablet, C. and Blaga, Radu and Rose, N. and Perret, Catherine and Đurković-Đaković, Olgica and Boireau, Pascal and Durand, B.",
year = "2016",
abstract = "Background: Infection by Toxoplasma gondii postnatally can occur after ingestion of contaminated meat or water (tissue cysts/oocysts). In Europe, percentage of meat borne infections is estimated between 30 and 63 %, out of which pork makes the most important source. The aim of this study was to (i) investigate the seroprevalence of T. gondii in intensive pig farms from western France; and (ii) identify the risk factors associated with seropositivity. Methods: Data were collected between November 2006 and February 2008 in 60 intensive farrow-to-finish farms, where sera were taken from 3595 fattening pigs, weaned and suckling piglets. Information about three classes of potential seropositivity risk factors were obtained through a questionnaire concerning: (i) breeding characteristics; (ii) farm management; and (iii) husbandry and hygiene. The modified agglutination test (MAT) was used for detection of specific anti T. gondii antibodies in pig sera, starting from 1/6 dilution. Results: The overall proportion of seropositive animals was 6.9 %, but the proportion of herds with at least one positive pig was 100 %. Multivariate logistic mixed model showed an increased seropositivity risk in weaned compared to suckling piglets, and a decreasing risk for mid-sized and large farms. The presence of a Danish entry facility, that clearly separates clean and dirty areas, had a protective effect on T. gondii seropositivity as well. Conclusions: The observed proportion of herds with at least one T. gondii seropositive animal provides further evidence that even in confined conditions of pig breeding, infection occurs, and is common. The highest risk for acquiring T. gondii is at the end of weaning period. Smaller confined pig farms demonstrate higher T. gondii seropositivity levels. This study also showed that Danish entry on farm buildings provides effective protection against T. gondii.",
publisher = "Biomed Central Ltd, London",
journal = "Parasites & Vectors",
title = "Factors associated with Toxoplasma gondii infection in confined farrow-to-finish pig herds in western France: an exploratory study in 60 herds",
volume = "9",
doi = "10.1186/s13071-016-1753-5"
}

Đokić, V., Fablet, C., Blaga, R., Rose, N., Perret, C., Đurković-Đaković, O., Boireau, P.,& Durand, B.. (2016). Factors associated with Toxoplasma gondii infection in confined farrow-to-finish pig herds in western France: an exploratory study in 60 herds. in Parasites & Vectors
Biomed Central Ltd, London., 9.
https://doi.org/10.1186/s13071-016-1753-5

Đokić V, Fablet C, Blaga R, Rose N, Perret C, Đurković-Đaković O, Boireau P, Durand B. Factors associated with Toxoplasma gondii infection in confined farrow-to-finish pig herds in western France: an exploratory study in 60 herds. in Parasites & Vectors. 2016;9.
doi:10.1186/s13071-016-1753-5 .

Đokić, Vitomir, Fablet, C., Blaga, Radu, Rose, N., Perret, Catherine, Đurković-Đaković, Olgica, Boireau, Pascal, Durand, B., "Factors associated with Toxoplasma gondii infection in confined farrow-to-finish pig herds in western France: an exploratory study in 60 herds" in Parasites & Vectors, 9 (2016),
https://doi.org/10.1186/s13071-016-1753-5 . .

TY  - JOUR
AU  - Đokić, Vitomir
AU  - Blaga, Radu
AU  - Aubert, Dominique
AU  - Durand, B.
AU  - Perret, Catherine
AU  - Geers, R.
AU  - Ducry, Tamara
AU  - Vallee, I.
AU  - Đurković-Đaković, Olgica
AU  - Mzabi, A.
AU  - Villena, Isabelle
AU  - Boireau, Pascal
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/716
AB  - The aim of this study was to assess the seroprevalence of the Toxoplasma gondii parasite in pork produced in France, and to determine infection risk factors. An innovative survey was designed based on annual numbers of slaughtered pigs from intensive and outdoor farms in France. A total of 1549 samples of cardiac fluids were collected from pig hearts to determine seroprevalence using a Modified Agglutination Test. Of those, 160 hearts were bio-assayed in mice to isolate live parasites. The overall seroprevalence among fattening pigs was 2.9%. The adjusted seroprevalence in pigs from intensive farms was 3.0%; the highest in sows (13.4%); 2.9% in fattening pigs and 2.6% in piglets. Adjusted seroprevalence in fattening animals from outdoor farms was 6.3%. Strains were isolated from 41 animals and all were genotyped by Restriction Fragment Length Polymorphism as type II. Risk-factor analysis showed that the risk of infection was more than three times higher for outdoor pigs, and that sows' risk was almost five times higher than that of fattening animals. This study provides further evidence of extensive pork infection with T. gondii regardless of breeding systems, indicating that farm conditions are still insufficient to guarantee 'Toxoplasma-free pork'.
PB  - Cambridge Univ Press, New York
T2  - Parasitology
T1  - Toxoplasma gondii infection in pork produced in France
EP  - 567
IS  - 5
SP  - 557
VL  - 143
DO  - 10.1017/S0031182015001870
ER  - 

@article{
author = "Đokić, Vitomir and Blaga, Radu and Aubert, Dominique and Durand, B. and Perret, Catherine and Geers, R. and Ducry, Tamara and Vallee, I. and Đurković-Đaković, Olgica and Mzabi, A. and Villena, Isabelle and Boireau, Pascal",
year = "2016",
abstract = "The aim of this study was to assess the seroprevalence of the Toxoplasma gondii parasite in pork produced in France, and to determine infection risk factors. An innovative survey was designed based on annual numbers of slaughtered pigs from intensive and outdoor farms in France. A total of 1549 samples of cardiac fluids were collected from pig hearts to determine seroprevalence using a Modified Agglutination Test. Of those, 160 hearts were bio-assayed in mice to isolate live parasites. The overall seroprevalence among fattening pigs was 2.9%. The adjusted seroprevalence in pigs from intensive farms was 3.0%; the highest in sows (13.4%); 2.9% in fattening pigs and 2.6% in piglets. Adjusted seroprevalence in fattening animals from outdoor farms was 6.3%. Strains were isolated from 41 animals and all were genotyped by Restriction Fragment Length Polymorphism as type II. Risk-factor analysis showed that the risk of infection was more than three times higher for outdoor pigs, and that sows' risk was almost five times higher than that of fattening animals. This study provides further evidence of extensive pork infection with T. gondii regardless of breeding systems, indicating that farm conditions are still insufficient to guarantee 'Toxoplasma-free pork'.",
publisher = "Cambridge Univ Press, New York",
journal = "Parasitology",
title = "Toxoplasma gondii infection in pork produced in France",
pages = "567-557",
number = "5",
volume = "143",
doi = "10.1017/S0031182015001870"
}

Đokić, V., Blaga, R., Aubert, D., Durand, B., Perret, C., Geers, R., Ducry, T., Vallee, I., Đurković-Đaković, O., Mzabi, A., Villena, I.,& Boireau, P.. (2016). Toxoplasma gondii infection in pork produced in France. in Parasitology
Cambridge Univ Press, New York., 143(5), 557-567.
https://doi.org/10.1017/S0031182015001870

Đokić V, Blaga R, Aubert D, Durand B, Perret C, Geers R, Ducry T, Vallee I, Đurković-Đaković O, Mzabi A, Villena I, Boireau P. Toxoplasma gondii infection in pork produced in France. in Parasitology. 2016;143(5):557-567.
doi:10.1017/S0031182015001870 .

Đokić, Vitomir, Blaga, Radu, Aubert, Dominique, Durand, B., Perret, Catherine, Geers, R., Ducry, Tamara, Vallee, I., Đurković-Đaković, Olgica, Mzabi, A., Villena, Isabelle, Boireau, Pascal, "Toxoplasma gondii infection in pork produced in France" in Parasitology, 143, no. 5 (2016):557-567,
https://doi.org/10.1017/S0031182015001870 . .

TY  - JOUR
AU  - Blaga, Radu
AU  - Aubert, Dominique
AU  - Perret, Catherine
AU  - Geers, R.
AU  - Đokić, Vitomir
AU  - Villena, Isabelle
AU  - Gilot-Fromont, E.
AU  - Mercier, Aurelien
AU  - Boireau, Pascal
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/609
AB  - Toxoplasmosis is a disease widely distributed throughout the world. It is caused by Toxoplasma gondii, a protozoan parasite, having the Felidae as final host (the cat) and warm-blooded vertebrates as intermediate hosts. Human infection is mainly orally, either by the ingestion of oocysts excreted with the cat faeces or by ingestion of tissue cysts which may be present in a wide variety of meat products. Direct transmission from a cat to its owner is probably less common; however the cat population represents the main source of environmental contamination. In France, nationwide studies that have been conducted in fresh mutton, beef and pork meat intended for human consumption, have highlight a seroprevalence of Toxoplasma gondii which varies according to animal species and age from 2.46% for piglets to 69.5% for adult sheep. The role of wildlife as hosts of the parasite should also to be considered in the epidemiology of toxoplasmosis firstly as a vector and secondly as a potential human source of contamination. In French Guyana, the hypothesis of the existence of a wild cycle of Toxoplasma gondii has been present for the last several years, involving the wild felids and their prey, mammals and birds, some of which beeing hunting products for humans.
T2  - Revue Francophone des Laboratoires
T1  - Animal reservoirs of Toxoplasma gondii : An update in France
T1  - Animaux réservoirs de Toxoplasma gondii: état des lieux en France
EP  - 52
IS  - 477
SP  - 35
VL  - 2015
DO  - 10.1016/S1773-035X(15)30315-4
ER  - 

@article{
author = "Blaga, Radu and Aubert, Dominique and Perret, Catherine and Geers, R. and Đokić, Vitomir and Villena, Isabelle and Gilot-Fromont, E. and Mercier, Aurelien and Boireau, Pascal",
year = "2015",
abstract = "Toxoplasmosis is a disease widely distributed throughout the world. It is caused by Toxoplasma gondii, a protozoan parasite, having the Felidae as final host (the cat) and warm-blooded vertebrates as intermediate hosts. Human infection is mainly orally, either by the ingestion of oocysts excreted with the cat faeces or by ingestion of tissue cysts which may be present in a wide variety of meat products. Direct transmission from a cat to its owner is probably less common; however the cat population represents the main source of environmental contamination. In France, nationwide studies that have been conducted in fresh mutton, beef and pork meat intended for human consumption, have highlight a seroprevalence of Toxoplasma gondii which varies according to animal species and age from 2.46% for piglets to 69.5% for adult sheep. The role of wildlife as hosts of the parasite should also to be considered in the epidemiology of toxoplasmosis firstly as a vector and secondly as a potential human source of contamination. In French Guyana, the hypothesis of the existence of a wild cycle of Toxoplasma gondii has been present for the last several years, involving the wild felids and their prey, mammals and birds, some of which beeing hunting products for humans.",
journal = "Revue Francophone des Laboratoires",
title = "Animal reservoirs of Toxoplasma gondii : An update in France, Animaux réservoirs de Toxoplasma gondii: état des lieux en France",
pages = "52-35",
number = "477",
volume = "2015",
doi = "10.1016/S1773-035X(15)30315-4"
}

Blaga, R., Aubert, D., Perret, C., Geers, R., Đokić, V., Villena, I., Gilot-Fromont, E., Mercier, A.,& Boireau, P.. (2015). Animal reservoirs of Toxoplasma gondii : An update in France. in Revue Francophone des Laboratoires, 2015(477), 35-52.
https://doi.org/10.1016/S1773-035X(15)30315-4

Blaga R, Aubert D, Perret C, Geers R, Đokić V, Villena I, Gilot-Fromont E, Mercier A, Boireau P. Animal reservoirs of Toxoplasma gondii : An update in France. in Revue Francophone des Laboratoires. 2015;2015(477):35-52.
doi:10.1016/S1773-035X(15)30315-4 .

Blaga, Radu, Aubert, Dominique, Perret, Catherine, Geers, R., Đokić, Vitomir, Villena, Isabelle, Gilot-Fromont, E., Mercier, Aurelien, Boireau, Pascal, "Animal reservoirs of Toxoplasma gondii : An update in France" in Revue Francophone des Laboratoires, 2015, no. 477 (2015):35-52,
https://doi.org/10.1016/S1773-035X(15)30315-4 . .

TY  - JOUR
AU  - Đokić, Vitomir
AU  - Blaga, Radu
AU  - Rinaldi, Laura
AU  - Le Roux, Delphine
AU  - Ducry, Tamara
AU  - Maurelli, Maria Paola
AU  - Perret, Catherine
AU  - Đurković-Đaković, Olgica
AU  - Cringoli, Giuseppe
AU  - Boireau, Pascal
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/532
AB  - Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P  lt  0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P  lt  0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental Parasitology
T1  - Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates
EP  - 71
SP  - 67
VL  - 147
DO  - 10.1016/j.exppara.2014.10.009
ER  - 

@article{
author = "Đokić, Vitomir and Blaga, Radu and Rinaldi, Laura and Le Roux, Delphine and Ducry, Tamara and Maurelli, Maria Paola and Perret, Catherine and Đurković-Đaković, Olgica and Cringoli, Giuseppe and Boireau, Pascal",
year = "2014",
abstract = "Oocysts of Toxoplasma gondii represent one of the most common environmental contaminants causing the zoonotic infection toxoplasmosis. The aim of the present study was to compare the Mini-FLOTAC device with traditional cell counting plates (Kova Slide) for the detection of T. gondii oocysts from feline feces. Two types of experiments were performed: (i) purified oocysts were counted in different dilutions and (ii) specific pathogen free T. gondii-negative cat feces was inoculated with numbers of purified oocysts and counting was performed directly from feces. Our analysis showed a thousand times higher sensitivity of Mini-FLOTAC (5 x 10(2) oocysts) compared to Kova Slide (5 x 10(5) oocysts). Also, when compared by McNemar's test, counting of the purified oocysts showed a higher sensitivity of Mini-FLOTAC compared to Kova Slide, for a dilution of 10(3) oocysts/ml (chi(2) = 6.1; P  lt  0.05). A better sensitivity was also found with Mini-FLOTAC in dilutions of 10(5) and 10(4) oocysts/ml, when counted from feces (chi(2) = 4.2 and 8.1, respectively, P  lt  0.05). Our results show that Mini-FLOTAC is more sensitive than traditional methods of T. gondii oocysts detection and quantification is more accurate. Furthermore, Mini-FLOTAC simplicity and cost effectiveness allow it to be used with light microscopes in any laboratory or field conditions. We therefore recommend its use for regular screening. Further studies are needed to validate Mini-FLOTAC for the detection of oocysts in soil and water samples in field conditions.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental Parasitology",
title = "Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates",
pages = "71-67",
volume = "147",
doi = "10.1016/j.exppara.2014.10.009"
}

Đokić, V., Blaga, R., Rinaldi, L., Le Roux, D., Ducry, T., Maurelli, M. P., Perret, C., Đurković-Đaković, O., Cringoli, G.,& Boireau, P.. (2014). Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates. in Experimental Parasitology
Academic Press Inc Elsevier Science, San Diego., 147, 67-71.
https://doi.org/10.1016/j.exppara.2014.10.009

Đokić V, Blaga R, Rinaldi L, Le Roux D, Ducry T, Maurelli MP, Perret C, Đurković-Đaković O, Cringoli G, Boireau P. Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates. in Experimental Parasitology. 2014;147:67-71.
doi:10.1016/j.exppara.2014.10.009 .

Đokić, Vitomir, Blaga, Radu, Rinaldi, Laura, Le Roux, Delphine, Ducry, Tamara, Maurelli, Maria Paola, Perret, Catherine, Đurković-Đaković, Olgica, Cringoli, Giuseppe, Boireau, Pascal, "Mini-FLOTAC for counting Toxoplasma gondii oocysts from cat feces - Comparison with cell counting plates" in Experimental Parasitology, 147 (2014):67-71,
https://doi.org/10.1016/j.exppara.2014.10.009 . .