TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Mitrović-Ajtić, Olivera
AU  - Jovčić, Gordana
AU  - Milošević, Maja
AU  - Momčilović, Sanja
AU  - Čokić, Vladan
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1029
AB  - Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress exposure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients.
PB  - Springer, New York
T2  - Histochemistry & Cell Biology
T1  - Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress
EP  - 468
IS  - 6
SP  - 457
VL  - 153
DO  - 10.1007/s00418-020-01856-y
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Budeč, Mirela and Marković, Dragana and Mitrović-Ajtić, Olivera and Jovčić, Gordana and Milošević, Maja and Momčilović, Sanja and Čokić, Vladan",
year = "2020",
abstract = "Anaemia occurs frequently in patients with heart failure and its current treatment lacks clear targets. Emerging evidence suggests that erythroid progenitor cell expansion is an integral part of physiological response to anaemia associated with chronic stress. Understanding the underlying mechanism may provide a novel approach to anaemia management. In this study, we aimed to examine a role for nitric oxide (NO) in the regulation of bone marrow erythroid progenitor response to chronic stress. For this purpose, adult male mice were subjected to 2 h daily restraint stress for 7 or 14 consecutive days. The role of NO was assessed by subcutaneous injection with NG-nitro-l-arginine methyl ester, 30 min prior to each restraint. Chronic exposure to stress resulted in significantly increased number of bone marrow erythroid progenitors, and blockade of NO biosynthesis prior to daily stress completely prevented stress-induced erythroid progenitor cell expansion. Furthermore, chronic stress exposure led to altered expression of neural, endothelial and inducible nitric oxide synthases (NOS) in the bone marrow, both on mRNA and protein level. Decreased expression of neural and endothelial NOS, as well as reduced expression of NF-kappaB/p65 in bone marrow nuclear cell fraction, was accompanied by elevated bone marrow expression of inducible NOS in chronically stressed animals. This is the first study to demonstrate a role for NO in adaptive response of erythroid progenitors to chronic stress. Targeting NO production may be beneficial to improve bone marrow dysfunction and reduced erythroid progenitor cell expansion in chronic heart failure patients.",
publisher = "Springer, New York",
journal = "Histochemistry & Cell Biology",
title = "Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress",
pages = "468-457",
number = "6",
volume = "153",
doi = "10.1007/s00418-020-01856-y"
}

Vignjević-Petrinović, S., Budeč, M., Marković, D., Mitrović-Ajtić, O., Jovčić, G., Milošević, M., Momčilović, S.,& Čokić, V.. (2020). Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress. in Histochemistry & Cell Biology
Springer, New York., 153(6), 457-468.
https://doi.org/10.1007/s00418-020-01856-y

Vignjević-Petrinović S, Budeč M, Marković D, Mitrović-Ajtić O, Jovčić G, Milošević M, Momčilović S, Čokić V. Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress. in Histochemistry & Cell Biology. 2020;153(6):457-468.
doi:10.1007/s00418-020-01856-y .

Vignjević-Petrinović, Sanja, Budeč, Mirela, Marković, Dragana, Mitrović-Ajtić, Olivera, Jovčić, Gordana, Milošević, Maja, Momčilović, Sanja, Čokić, Vladan, "Nitric oxide-dependent expansion of erythroid progenitors in a murine model of chronic psychological stress" in Histochemistry & Cell Biology, 153, no. 6 (2020):457-468,
https://doi.org/10.1007/s00418-020-01856-y . .

TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Gotić, Mirjana
AU  - Mitrović-Ajtić, Olivera
AU  - Mojsilović, Slavko
AU  - Stošić-Grujičić, Stanislava
AU  - Ivanov, Milan
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/745
AB  - Macrophage migration inhibitory factor is a well-known proinflammatory cytokine that is released during systemic stress response. Although MIF can affect erythrocyte production, the role of this cytokine in stress-induced erythropoiesis is completely unknown. To extend our previous findings showing that chronic psychological stress stimulates extramedullary erythropoiesis, here we examined whether MIF is involved in the control of stress-induced erythropoietic response. Adult male C57BL/6 wild-type (WT) and MIF-KO (knock-out) mice were subjected to 2-h daily restraint stress for either 7 or 14 consecutive days. The number of erythroid progenitors and CD71/Ter119 profile of erythroid precursors were analyzed in the bone marrow and spleen. Additionally, MIF protein expression was assessed in WT mice. Our results demonstrated that chronic restraint stress enhanced the number of both erythroid progenitors and precursors in the spleen. Stress-induced increase in the number of splenic late erythroid progenitors as well as in the percentage of CD71(+)Ter119(+)-double-positive precursors was significantly more pronounced in MIF-KO mice compared to WT animals. Furthermore, repeatedly stressed WT animals demonstrated an augmented MIF expression in the spleen. Unlike the spleen, the bone marrow of chronically stressed WT mice exhibited less prominent changes in erythropoietic stress response and no significant alteration in MIF expression. In addition, MIF deficiency did not influence the bone marrow erythropoiesis in stressed animals. These findings suggest that MIF regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions.
PB  - Springer, New York
T2  - Histochemistry & Cell Biology
T1  - Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis
EP  - 324
IS  - 3
SP  - 311
VL  - 146
DO  - 10.1007/s00418-016-1442-7
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Budeč, Mirela and Marković, Dragana and Gotić, Mirjana and Mitrović-Ajtić, Olivera and Mojsilović, Slavko and Stošić-Grujičić, Stanislava and Ivanov, Milan and Jovčić, Gordana and Čokić, Vladan",
year = "2016",
abstract = "Macrophage migration inhibitory factor is a well-known proinflammatory cytokine that is released during systemic stress response. Although MIF can affect erythrocyte production, the role of this cytokine in stress-induced erythropoiesis is completely unknown. To extend our previous findings showing that chronic psychological stress stimulates extramedullary erythropoiesis, here we examined whether MIF is involved in the control of stress-induced erythropoietic response. Adult male C57BL/6 wild-type (WT) and MIF-KO (knock-out) mice were subjected to 2-h daily restraint stress for either 7 or 14 consecutive days. The number of erythroid progenitors and CD71/Ter119 profile of erythroid precursors were analyzed in the bone marrow and spleen. Additionally, MIF protein expression was assessed in WT mice. Our results demonstrated that chronic restraint stress enhanced the number of both erythroid progenitors and precursors in the spleen. Stress-induced increase in the number of splenic late erythroid progenitors as well as in the percentage of CD71(+)Ter119(+)-double-positive precursors was significantly more pronounced in MIF-KO mice compared to WT animals. Furthermore, repeatedly stressed WT animals demonstrated an augmented MIF expression in the spleen. Unlike the spleen, the bone marrow of chronically stressed WT mice exhibited less prominent changes in erythropoietic stress response and no significant alteration in MIF expression. In addition, MIF deficiency did not influence the bone marrow erythropoiesis in stressed animals. These findings suggest that MIF regulates extramedullary erythropoiesis by inhibiting an overexpansion of splenic immature erythroid cells during chronic stress and indicate a novel role for this cytokine under chronic stress conditions.",
publisher = "Springer, New York",
journal = "Histochemistry & Cell Biology",
title = "Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis",
pages = "324-311",
number = "3",
volume = "146",
doi = "10.1007/s00418-016-1442-7"
}

Vignjević-Petrinović, S., Budeč, M., Marković, D., Gotić, M., Mitrović-Ajtić, O., Mojsilović, S., Stošić-Grujičić, S., Ivanov, M., Jovčić, G.,& Čokić, V.. (2016). Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. in Histochemistry & Cell Biology
Springer, New York., 146(3), 311-324.
https://doi.org/10.1007/s00418-016-1442-7

Vignjević-Petrinović S, Budeč M, Marković D, Gotić M, Mitrović-Ajtić O, Mojsilović S, Stošić-Grujičić S, Ivanov M, Jovčić G, Čokić V. Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis. in Histochemistry & Cell Biology. 2016;146(3):311-324.
doi:10.1007/s00418-016-1442-7 .

Vignjević-Petrinović, Sanja, Budeč, Mirela, Marković, Dragana, Gotić, Mirjana, Mitrović-Ajtić, Olivera, Mojsilović, Slavko, Stošić-Grujičić, Stanislava, Ivanov, Milan, Jovčić, Gordana, Čokić, Vladan, "Macrophage migration inhibitory factor is an endogenous regulator of stress-induced extramedullary erythropoiesis" in Histochemistry & Cell Biology, 146, no. 3 (2016):311-324,
https://doi.org/10.1007/s00418-016-1442-7 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Santibanez, Juan F.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/710
AB  - Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 inmyoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulateMMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.
PB  - Hindawi Ltd, London
T2  - Mediators of Inflammation
T1  - Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation
VL  - 2016
DO  - 10.1155/2016/2939658
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Mojsilović, Slavko and Jauković, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Santibanez, Juan F.",
year = "2016",
abstract = "Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 inmyoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulateMMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.",
publisher = "Hindawi Ltd, London",
journal = "Mediators of Inflammation",
title = "Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation",
volume = "2016",
doi = "10.1155/2016/2939658"
}

Obradović, H., Krstić, J., Kukolj, T., Trivanović, D., Okić Đorđević, I., Mojsilović, S., Jauković, A., Jovčić, G., Bugarski, D.,& Santibanez, J. F.. (2016). Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation. in Mediators of Inflammation
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/2939658

Obradović H, Krstić J, Kukolj T, Trivanović D, Okić Đorđević I, Mojsilović S, Jauković A, Jovčić G, Bugarski D, Santibanez JF. Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation. in Mediators of Inflammation. 2016;2016.
doi:10.1155/2016/2939658 .

Obradović, Hristina, Krstić, Jelena, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Mojsilović, Slavko, Jauković, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Santibanez, Juan F., "Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation" in Mediators of Inflammation, 2016 (2016),
https://doi.org/10.1155/2016/2939658 . .

TY  - JOUR
AU  - Mitrović-Ajtić, Olivera
AU  - Todorović, Slobodan
AU  - Diklić, Miloš
AU  - Subotički, Tijana
AU  - Beleslin-Čokić, Bojana
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/740
AB  - Background/aim: The purpose of this study was to investigate proliferation and differentiation markers in colorectal adenocarcinoma and their correlation with clinicopathological factors. Materials and methods: Samples were collected from 38 patients with colorectal adenocarcinoma and 10 healthy controls. E-cadherin, carcinoembryonic antigen (mCEA), cyclin B1, vascular endothelial growth factor (VEGF), and erythropoietin (EPO) receptor (EPOR) were examined by immunohistochemistry; VEGF and EPO were examined by real-time PCR. Results: The tumor samples were mostly characterized by large dimension (pT3), moderate level of differentiation (G2), negative lymph node status (N0), and no metastasis. Cyclin B1 and VEGF gene and protein expressions were significantly higher in tumor tissues than in control tissues; E-cadherin expression was significantly decreased in tumor samples and in positive correlation with mCEA. EPO was almost undetectable in tumor tissues of colorectal adenocarcinoma. Significant positive correlation was detected between tumor size and cyclin B1, tumor grade, and lymph node status. Conclusion: Decreased expression of EPO, high levels of VEGF and cyclin B1 expression, predominant moderate tumor differentiation, absence of metastasis, and negative lymph node status may suggest low level of aggressiveness, better prognosis, and longer patient survival.
PB  - Tubitak Scientific & Technical Research Council Turkey, Ankara
T2  - Turkish Journal of Medical Sciences
T1  - Proliferation and differentiation markers of colorectal adenocarcinoma and their correlation with clinicopathological factors
EP  - 1176
IS  - 4
SP  - 1168
VL  - 46
DO  - 10.3906/sag-1412-85
ER  - 

@article{
author = "Mitrović-Ajtić, Olivera and Todorović, Slobodan and Diklić, Miloš and Subotički, Tijana and Beleslin-Čokić, Bojana and Jovčić, Gordana and Čokić, Vladan",
year = "2016",
abstract = "Background/aim: The purpose of this study was to investigate proliferation and differentiation markers in colorectal adenocarcinoma and their correlation with clinicopathological factors. Materials and methods: Samples were collected from 38 patients with colorectal adenocarcinoma and 10 healthy controls. E-cadherin, carcinoembryonic antigen (mCEA), cyclin B1, vascular endothelial growth factor (VEGF), and erythropoietin (EPO) receptor (EPOR) were examined by immunohistochemistry; VEGF and EPO were examined by real-time PCR. Results: The tumor samples were mostly characterized by large dimension (pT3), moderate level of differentiation (G2), negative lymph node status (N0), and no metastasis. Cyclin B1 and VEGF gene and protein expressions were significantly higher in tumor tissues than in control tissues; E-cadherin expression was significantly decreased in tumor samples and in positive correlation with mCEA. EPO was almost undetectable in tumor tissues of colorectal adenocarcinoma. Significant positive correlation was detected between tumor size and cyclin B1, tumor grade, and lymph node status. Conclusion: Decreased expression of EPO, high levels of VEGF and cyclin B1 expression, predominant moderate tumor differentiation, absence of metastasis, and negative lymph node status may suggest low level of aggressiveness, better prognosis, and longer patient survival.",
publisher = "Tubitak Scientific & Technical Research Council Turkey, Ankara",
journal = "Turkish Journal of Medical Sciences",
title = "Proliferation and differentiation markers of colorectal adenocarcinoma and their correlation with clinicopathological factors",
pages = "1176-1168",
number = "4",
volume = "46",
doi = "10.3906/sag-1412-85"
}

Mitrović-Ajtić, O., Todorović, S., Diklić, M., Subotički, T., Beleslin-Čokić, B., Jovčić, G.,& Čokić, V.. (2016). Proliferation and differentiation markers of colorectal adenocarcinoma and their correlation with clinicopathological factors. in Turkish Journal of Medical Sciences
Tubitak Scientific & Technical Research Council Turkey, Ankara., 46(4), 1168-1176.
https://doi.org/10.3906/sag-1412-85

Mitrović-Ajtić O, Todorović S, Diklić M, Subotički T, Beleslin-Čokić B, Jovčić G, Čokić V. Proliferation and differentiation markers of colorectal adenocarcinoma and their correlation with clinicopathological factors. in Turkish Journal of Medical Sciences. 2016;46(4):1168-1176.
doi:10.3906/sag-1412-85 .

Mitrović-Ajtić, Olivera, Todorović, Slobodan, Diklić, Miloš, Subotički, Tijana, Beleslin-Čokić, Bojana, Jovčić, Gordana, Čokić, Vladan, "Proliferation and differentiation markers of colorectal adenocarcinoma and their correlation with clinicopathological factors" in Turkish Journal of Medical Sciences, 46, no. 4 (2016):1168-1176,
https://doi.org/10.3906/sag-1412-85 . .

TY  - JOUR
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Diklić, Miloš
AU  - Subotički, Tijana
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/653
AB  - Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellularity of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.
PB  - Polish Physiological Soc, Grzegorzecka
T2  - Journal of Physiology & Pharmacology
T1  - Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress
EP  - 100
IS  - 1
SP  - 91
VL  - 66
UR  - https://hdl.handle.net/21.15107/rcub_rimi_653
ER  - 

@article{
author = "Vignjević, Sanja and Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Diklić, Miloš and Subotički, Tijana and Čokić, Vladan and Jovčić, Gordana",
year = "2015",
abstract = "Stress evokes an integrated neuroendocrine response perturbing the homeostasis of different physiological systems. In contrast to well established physiologica linteractions between neuroendocrine and immune systems during chronic stress, there has been relatively little information on the effects of psychological stress on erythroid cells. Since stress-induced erythropoiesis occurs predominantly in the spleen, in the current study, we investigated the influence of chronic psychological stress on splenic erythroid progenitors and examined a role of glucocorticoid receptor (OR) in observed effect using a mouse model of restraint. The adult male mice were subjected to 2 hours daily restraint stress for 7 or 14 consecutive days and the role of OR in erythropoietic response to stress was assessed by pretreatment of mice with OR antagonist mifepristone 60 min prior to restraint. The results showed that chronic restraint stress induced an increase in spleen weight as well as in the cellularity of red pulp, as compared to controls. Furthermore, 7 and 14 days of restraint stress resulted in markedly increased number of both splenic early (BFU-E) and late (CFU-E) erythroid progenitors. Blockade of OR with mifepristone did not affect the number of BFU-E in stressed mice, but it completely abolished the effect of repeated psychological stress on CFU-E cells. Additionally, plasma corticosterone concentration was enhanced whereas the OR expression was significantly decreased within splenic red pulp after one and two weeks of stress exposure. Obtained findings suggest for the first time an indispensable role for OR in the expansion of CFU-E progenitors in the spleen under conditions of chronic psychological stress.",
publisher = "Polish Physiological Soc, Grzegorzecka",
journal = "Journal of Physiology & Pharmacology",
title = "Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress",
pages = "100-91",
number = "1",
volume = "66",
url = "https://hdl.handle.net/21.15107/rcub_rimi_653"
}

Vignjević, S., Budeč, M., Marković, D., Đikić, D., Mitrović, O., Diklić, M., Subotički, T., Čokić, V.,& Jovčić, G.. (2015). Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress. in Journal of Physiology & Pharmacology
Polish Physiological Soc, Grzegorzecka., 66(1), 91-100.
https://hdl.handle.net/21.15107/rcub_rimi_653

Vignjević S, Budeč M, Marković D, Đikić D, Mitrović O, Diklić M, Subotički T, Čokić V, Jovčić G. Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress. in Journal of Physiology & Pharmacology. 2015;66(1):91-100.
https://hdl.handle.net/21.15107/rcub_rimi_653 .

Vignjević, Sanja, Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Mitrović, Olivera, Diklić, Miloš, Subotički, Tijana, Čokić, Vladan, Jovčić, Gordana, "Glucocorticoid receptor mediates the expansion of splenic late erythroid progenitors during chronic psychological stress" in Journal of Physiology & Pharmacology, 66, no. 1 (2015):91-100,
https://hdl.handle.net/21.15107/rcub_rimi_653 .

TY  - CONF
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Marković, Dragana
AU  - Čokić, Vladan
AU  - Mitrović-Ajtić, Olivera
AU  - Diklić, Miloš
AU  - Jovčić, Gordana
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/667
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Chronic psychological stress stimulates medullary erythropoiesis by modulating local nitric oxide production
EP  - 129
SP  - 129
VL  - 100
UR  - https://hdl.handle.net/21.15107/rcub_rimi_667
ER  - 

@conference{
author = "Budeč, Mirela and Vignjević, Sanja and Marković, Dragana and Čokić, Vladan and Mitrović-Ajtić, Olivera and Diklić, Miloš and Jovčić, Gordana",
year = "2015",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Chronic psychological stress stimulates medullary erythropoiesis by modulating local nitric oxide production",
pages = "129-129",
volume = "100",
url = "https://hdl.handle.net/21.15107/rcub_rimi_667"
}

Budeč, M., Vignjević, S., Marković, D., Čokić, V., Mitrović-Ajtić, O., Diklić, M.,& Jovčić, G.. (2015). Chronic psychological stress stimulates medullary erythropoiesis by modulating local nitric oxide production. in Haematologica
Ferrata Storti Foundation, Pavia., 100, 129-129.
https://hdl.handle.net/21.15107/rcub_rimi_667

Budeč M, Vignjević S, Marković D, Čokić V, Mitrović-Ajtić O, Diklić M, Jovčić G. Chronic psychological stress stimulates medullary erythropoiesis by modulating local nitric oxide production. in Haematologica. 2015;100:129-129.
https://hdl.handle.net/21.15107/rcub_rimi_667 .

Budeč, Mirela, Vignjević, Sanja, Marković, Dragana, Čokić, Vladan, Mitrović-Ajtić, Olivera, Diklić, Miloš, Jovčić, Gordana, "Chronic psychological stress stimulates medullary erythropoiesis by modulating local nitric oxide production" in Haematologica, 100 (2015):129-129,
https://hdl.handle.net/21.15107/rcub_rimi_667 .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Nikolić, Srdjan
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Okić Đorđević, Ivana
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/602
AB  - Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.
PB  - Wiley, Hoboken
T2  - Cell Biology International
T1  - Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro
EP  - 265
IS  - 2
SP  - 254
VL  - 38
DO  - 10.1002/cbin.10198
ER  - 

@article{
author = "Trivanović, Drenka and Nikolić, Srdjan and Krstić, Jelena and Jauković, Aleksandra and Mojsilović, Slavko and Ilić, Vesna and Okić Đorđević, Ivana and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Adipose tissue is an attractive source of mesenchymal stem/stromal cells (MSCs) with potential applications in reconstructive plastic surgery and regenerative medicine. The aim of this study was to characterise human adipose tissue MSCs (ASCs) derived from healthy individuals and cancer patients and to compare their interactions with tumour cells. ASCs were isolated from adipose tissue of healthy donors, breast cancer-adjacent adipose tissue of breast cancer patients and tumour-adjacent adipose tissue of non-breast cancer patients. Their proliferation, differentiation, immunophenotype and gene expression were assessed and effects on the proliferation of human breast cancer cell line MCF-7 compared. ASCs from all sources exhibited similar morphology, proliferative and differentiation potential, showing the characteristic pattern of mesenchymal surface markers expression (CD90, CD105, CD44H, CD73) and the lack of HLA-DR and hematopoietic markers (CD11a, CD33, CD45, Glycophorin-CD235a), but uneven expression of CD34. ASCs also shared a common positive gene expression of HLA-DR, HLA-A, IL-6, TGF- and HIF-1, but were negative for HLA-G, while the expression levels of Cox-2 and IDO-1 varied. All ASCs significantly stimulated the proliferation of MCF-7 tumour cells in direct mixed co-cultures and transwell system, although their conditioned media displayed antiproliferative activity. Data obtained showed that ASCs with similar characteristics are easily isolated from various donors and sites of origin, although ASCs could both suppress and favour tumour cells growth, emphasising the importance of cellular context within the microenvironment and pointing to the significance of safety studies to exclude any potential clinical risk of their application in regenerative medicine.",
publisher = "Wiley, Hoboken",
journal = "Cell Biology International",
title = "Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro",
pages = "265-254",
number = "2",
volume = "38",
doi = "10.1002/cbin.10198"
}

Trivanović, D., Nikolić, S., Krstić, J., Jauković, A., Mojsilović, S., Ilić, V., Okić Đorđević, I., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. in Cell Biology International
Wiley, Hoboken., 38(2), 254-265.
https://doi.org/10.1002/cbin.10198

Trivanović D, Nikolić S, Krstić J, Jauković A, Mojsilović S, Ilić V, Okić Đorđević I, Santibanez JF, Jovčić G, Bugarski D. Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro. in Cell Biology International. 2014;38(2):254-265.
doi:10.1002/cbin.10198 .

Trivanović, Drenka, Nikolić, Srdjan, Krstić, Jelena, Jauković, Aleksandra, Mojsilović, Slavko, Ilić, Vesna, Okić Đorđević, Ivana, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Characteristics of human adipose mesenchymal stem cells isolated from healthy and cancer affected people and their interactions with human breast cancer cell line MCF-7 in vitro" in Cell Biology International, 38, no. 2 (2014):254-265,
https://doi.org/10.1002/cbin.10198 . .

TY  - CONF
AU  - Diklić, Miloš
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Milanović, S.
AU  - Mojsilović, S.
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/556
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Oxidative stress in myeloproliferative neoplasms
EP  - 662
SP  - 662
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1087
ER  - 

@conference{
author = "Diklić, Miloš and Marković, Dragana and Đikić, Dragoslava and Milanović, S. and Mojsilović, S. and Jovčić, Gordana and Čokić, Vladan",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Oxidative stress in myeloproliferative neoplasms",
pages = "662-662",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1087"
}

Diklić, M., Marković, D., Đikić, D., Milanović, S., Mojsilović, S., Jovčić, G.,& Čokić, V.. (2014). Oxidative stress in myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 662-662.
https://hdl.handle.net/21.15107/rcub_veterinar_1087

Diklić M, Marković D, Đikić D, Milanović S, Mojsilović S, Jovčić G, Čokić V. Oxidative stress in myeloproliferative neoplasms. in Haematologica. 2014;99:662-662.
https://hdl.handle.net/21.15107/rcub_veterinar_1087 .

Diklić, Miloš, Marković, Dragana, Đikić, Dragoslava, Milanović, S., Mojsilović, S., Jovčić, Gordana, Čokić, Vladan, "Oxidative stress in myeloproliferative neoplasms" in Haematologica, 99 (2014):662-662,
https://hdl.handle.net/21.15107/rcub_veterinar_1087 .

TY  - JOUR
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Mojsilović, Slavko
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Beleslin-Čokić, Bojana
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/575
AB  - Psychological stress affects different physiological processes including haematopoiesis. However, erythropoietic effects of chronic psychological stress remain largely unknown. The adult spleen contains a distinct microenvironment favourable for rapid expansion of erythroid progenitors in response to stressful stimuli, and emerging evidence suggests that inappropriate activation of stress erythropoiesis may predispose to leukaemic transformation. We used a mouse model to study the influence of chronic psychological stress on erythropoiesis in the spleen and to investigate potential mediators of observed effects. Adult mice were subjected to 2hrs daily restraint stress for 7 or 14 consecutive days. Our results showed that chronic exposure to restraint stress decreased the concentration of haemoglobin in the blood, elevated circulating levels of erythropoietin and corticosterone, and resulted in markedly increased number of erythroid progenitors and precursors in the spleen. Western blot analysis revealed significantly decreased expression of both erythropoietin receptor and glucocorticoid receptor in the spleen of restrained mice. Furthermore, chronic stress enhanced the expression of stem cell factor receptor in the red pulp. Moreover, chronically stressed animals exhibited significantly increased expression of bone morphogenetic protein 4 (BMP4) in the red pulp as well as substantially enhanced mRNA expression levels of its receptors in the spleen. These findings demonstrate for the first time that chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis and leads to the prolonged activation of stress erythropoiesis pathways. Prolonged activation of these pathways along with an excessive production of immature erythroid cells may predispose chronically stressed subjects to a higher risk of leukaemic transformation.
PB  - Wiley, Hoboken
T2  - Journal of Cellular & Molecular Medicine
T1  - Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis
EP  - 103
IS  - 1
SP  - 91
VL  - 18
DO  - 10.1111/jcmm.12167
ER  - 

@article{
author = "Vignjević, Sanja and Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Mojsilović, Slavko and Vranješ-Đurić, Sanja and Koko, Vesna and Beleslin-Čokić, Bojana and Čokić, Vladan and Jovčić, Gordana",
year = "2014",
abstract = "Psychological stress affects different physiological processes including haematopoiesis. However, erythropoietic effects of chronic psychological stress remain largely unknown. The adult spleen contains a distinct microenvironment favourable for rapid expansion of erythroid progenitors in response to stressful stimuli, and emerging evidence suggests that inappropriate activation of stress erythropoiesis may predispose to leukaemic transformation. We used a mouse model to study the influence of chronic psychological stress on erythropoiesis in the spleen and to investigate potential mediators of observed effects. Adult mice were subjected to 2hrs daily restraint stress for 7 or 14 consecutive days. Our results showed that chronic exposure to restraint stress decreased the concentration of haemoglobin in the blood, elevated circulating levels of erythropoietin and corticosterone, and resulted in markedly increased number of erythroid progenitors and precursors in the spleen. Western blot analysis revealed significantly decreased expression of both erythropoietin receptor and glucocorticoid receptor in the spleen of restrained mice. Furthermore, chronic stress enhanced the expression of stem cell factor receptor in the red pulp. Moreover, chronically stressed animals exhibited significantly increased expression of bone morphogenetic protein 4 (BMP4) in the red pulp as well as substantially enhanced mRNA expression levels of its receptors in the spleen. These findings demonstrate for the first time that chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis and leads to the prolonged activation of stress erythropoiesis pathways. Prolonged activation of these pathways along with an excessive production of immature erythroid cells may predispose chronically stressed subjects to a higher risk of leukaemic transformation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular & Molecular Medicine",
title = "Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis",
pages = "103-91",
number = "1",
volume = "18",
doi = "10.1111/jcmm.12167"
}

Vignjević, S., Budeč, M., Marković, D., Đikić, D., Mitrović, O., Mojsilović, S., Vranješ-Đurić, S., Koko, V., Beleslin-Čokić, B., Čokić, V.,& Jovčić, G.. (2014). Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. in Journal of Cellular & Molecular Medicine
Wiley, Hoboken., 18(1), 91-103.
https://doi.org/10.1111/jcmm.12167

Vignjević S, Budeč M, Marković D, Đikić D, Mitrović O, Mojsilović S, Vranješ-Đurić S, Koko V, Beleslin-Čokić B, Čokić V, Jovčić G. Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. in Journal of Cellular & Molecular Medicine. 2014;18(1):91-103.
doi:10.1111/jcmm.12167 .

Vignjević, Sanja, Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Mitrović, Olivera, Mojsilović, Slavko, Vranješ-Đurić, Sanja, Koko, Vesna, Beleslin-Čokić, Bojana, Čokić, Vladan, Jovčić, Gordana, "Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis" in Journal of Cellular & Molecular Medicine, 18, no. 1 (2014):91-103,
https://doi.org/10.1111/jcmm.12167 . .

TY  - CONF
AU  - Subotički, Tijana
AU  - Mitrović, Olivera
AU  - Diklić, Miloš
AU  - Mićić, Mileva
AU  - Mojsilović, S.
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/557
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis
EP  - 729
SP  - 728
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimi_557
ER  - 

@conference{
author = "Subotički, Tijana and Mitrović, Olivera and Diklić, Miloš and Mićić, Mileva and Mojsilović, S. and Vignjević, Sanja and Budeč, Mirela and Jovčić, Gordana and Čokić, Vladan",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis",
pages = "729-728",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_rimi_557"
}

Subotički, T., Mitrović, O., Diklić, M., Mićić, M., Mojsilović, S., Vignjević, S., Budeč, M., Jovčić, G.,& Čokić, V.. (2014). Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 728-729.
https://hdl.handle.net/21.15107/rcub_rimi_557

Subotički T, Mitrović O, Diklić M, Mićić M, Mojsilović S, Vignjević S, Budeč M, Jovčić G, Čokić V. Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis. in Haematologica. 2014;99:728-729.
https://hdl.handle.net/21.15107/rcub_rimi_557 .

Subotički, Tijana, Mitrović, Olivera, Diklić, Miloš, Mićić, Mileva, Mojsilović, S., Vignjević, Sanja, Budeč, Mirela, Jovčić, Gordana, Čokić, Vladan, "Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis" in Haematologica, 99 (2014):728-729,
https://hdl.handle.net/21.15107/rcub_rimi_557 .

TY  - JOUR
AU  - Čokić, Vladan
AU  - Subotički, Tijana
AU  - Beleslin-Čokić, Bojana
AU  - Diklić, Miloš
AU  - Milenković, Pavle B.
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/590
AB  - Introduction Hydroxycarbamide, used in therapy of hemoglobinopathies, enhances nitric oxide (NO) production both in primary human umbilical vein endothelial cells (HUVECs) and human bone marrow endothelial cell line (TrHBMEC). Moreover, NO increases γ-globin and fetal hemoglobin levels in human erythroid progenitors. Objective In order to find out whether simple physiologic stimulation of NO production by components of hematopoietic microenvironment can increase γ-globin gene expression, the effects of NO-inducer bradykinin were examined in endothelial cells. Methods The study was performed in co-cultures of human erythroid progenitors, TrHBMEC and HUVECs by ozone-based chemiluminescent determination of NO and real-time quantitative RT-PCR. Results In accordance with previous reports, the endogenous factor bradykinin increased endothelial cell production of NO in a dose- and time-dependent manner (0.1-0.6 μM up to 30 minutes). This induction of NO in HUVECs and TrHBMEC by bradykinin was blocked by competitive inhibitors of NO synthase (NOS), demonstrating NOS-dependence. It has been shown that bradykinin significantly reduced endothelial NOS (eNOS) mRNA level and eNOS/s-actin ratio in HUVEC (by twofold). In addition, bradykinin failed to increase γ-globin mRNA expression in erythroid progenitors only, as well as in co-culture studies of erythroid progenitors with TrHBMEC and HUVEC after 24 hours of treatment. Furthermore, bradykinin did not induce γ/β globin ratio in erythroid progenitors in co-cultures with HUVEC. Conclusion Bradykinin mediated eNOS activation leads to short time and low NO production in endothelial cells, insufficient to induce γ-globin gene expression. These results emphasized the significance of elevated and extended NO production in augmentation of γ-globin gene expression.
AB  - Uvod Hidroksikarbamid, koji se koristi u lečenju hemoglobinopatija, podstiče stvaranje azot-monoksida (NO) kako u primarnim ljudskim endotelnim ćelijama pupčane vene (HUVEC), tako i u izmenjenoj endotelnoj ćelijskoj liniji poreklom iz koštane srži (TrHBMEC). Štaviše, NO povećava stvaranje γ-globina i fetalnog hemoglobina u ljudskim progenitorima eritropoeze. Cilj rada Da bismo ustanovili da li jednostavna fiziološka stimulacija stvaranja NO od komponenti mikrosredine hematopoeze može povećati ekspresiju γ-globinskog gena, ispitivali smo efekte bradikinina, već poznatog stimulatora stvaranja NO. Metode rada Studija je izvedena u zajedničkim kulturama ljudskih progenitora eritropoeze sa TrHBMEC ili HUVEC i ispitivana hemiluminiscentnim merenjem NO posredstvom ozona, kao i primenom kvantitativnog RT-PCR na genskom nivou. Rezultati U skladu s prethodnim izveštajima, pokazali smo da endogeni faktor bradikinin povećava stvaranje NO u endotelnim ćelijama na dozno i vremenski zavisan način (0,1-0,6 μM do 30 minuta). Ovo stvaranje NO u HUVEC i TrHBMEC izazvano bradikininom blokirano je od strane konkurentskih inhibitora NO-sintaze (NOS), pokazujući NOS-zavisnost. Utvrdili smo da bradikinin značajno smanjuje stvaranje iRNK endotelne forme NOS (eNOS), kao i odnos eNOS i β-aktina u HUVEC (dvostruko manje). Pored toga, bradikinin ne povećava ekspresiju iRNK γ-globinskog gena ni u zasebnim progenitorima eritropoeze, niti u zajedničkim kulturama progenitora eritropoeze sa TrHBMEC ili HUVEC posle 24 sata tretmana. Bradikinin ne menja ni odnos γ i β globina u zajedničkim kulturama progenitora eritropoeze sa HUVEC. Zaključak Aktivacija eNO_ izazvana bradikininom dovodi do kratkog i malog povećanja NO u endotelnim ćelijama, što je nedovoljno da podstakne ekspresiju gena za γ-globin. Ovi rezultati naglašavaju važnost povećanog i produženog stvaranja NO radi stimulacije ekspresije γ-globina.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Bradykinin stimulation of nitric oxide production is not sufficient for gamma-globin induction
T1  - Stvaranje azot-monoksida izazvano bradikininom nije dovoljno za indukciju gama-globina
EP  - 196
IS  - 3-4
SP  - 189
VL  - 142
DO  - 10.2298/SARH1404189C
ER  - 

@article{
author = "Čokić, Vladan and Subotički, Tijana and Beleslin-Čokić, Bojana and Diklić, Miloš and Milenković, Pavle B. and Jovčić, Gordana",
year = "2014",
abstract = "Introduction Hydroxycarbamide, used in therapy of hemoglobinopathies, enhances nitric oxide (NO) production both in primary human umbilical vein endothelial cells (HUVECs) and human bone marrow endothelial cell line (TrHBMEC). Moreover, NO increases γ-globin and fetal hemoglobin levels in human erythroid progenitors. Objective In order to find out whether simple physiologic stimulation of NO production by components of hematopoietic microenvironment can increase γ-globin gene expression, the effects of NO-inducer bradykinin were examined in endothelial cells. Methods The study was performed in co-cultures of human erythroid progenitors, TrHBMEC and HUVECs by ozone-based chemiluminescent determination of NO and real-time quantitative RT-PCR. Results In accordance with previous reports, the endogenous factor bradykinin increased endothelial cell production of NO in a dose- and time-dependent manner (0.1-0.6 μM up to 30 minutes). This induction of NO in HUVECs and TrHBMEC by bradykinin was blocked by competitive inhibitors of NO synthase (NOS), demonstrating NOS-dependence. It has been shown that bradykinin significantly reduced endothelial NOS (eNOS) mRNA level and eNOS/s-actin ratio in HUVEC (by twofold). In addition, bradykinin failed to increase γ-globin mRNA expression in erythroid progenitors only, as well as in co-culture studies of erythroid progenitors with TrHBMEC and HUVEC after 24 hours of treatment. Furthermore, bradykinin did not induce γ/β globin ratio in erythroid progenitors in co-cultures with HUVEC. Conclusion Bradykinin mediated eNOS activation leads to short time and low NO production in endothelial cells, insufficient to induce γ-globin gene expression. These results emphasized the significance of elevated and extended NO production in augmentation of γ-globin gene expression., Uvod Hidroksikarbamid, koji se koristi u lečenju hemoglobinopatija, podstiče stvaranje azot-monoksida (NO) kako u primarnim ljudskim endotelnim ćelijama pupčane vene (HUVEC), tako i u izmenjenoj endotelnoj ćelijskoj liniji poreklom iz koštane srži (TrHBMEC). Štaviše, NO povećava stvaranje γ-globina i fetalnog hemoglobina u ljudskim progenitorima eritropoeze. Cilj rada Da bismo ustanovili da li jednostavna fiziološka stimulacija stvaranja NO od komponenti mikrosredine hematopoeze može povećati ekspresiju γ-globinskog gena, ispitivali smo efekte bradikinina, već poznatog stimulatora stvaranja NO. Metode rada Studija je izvedena u zajedničkim kulturama ljudskih progenitora eritropoeze sa TrHBMEC ili HUVEC i ispitivana hemiluminiscentnim merenjem NO posredstvom ozona, kao i primenom kvantitativnog RT-PCR na genskom nivou. Rezultati U skladu s prethodnim izveštajima, pokazali smo da endogeni faktor bradikinin povećava stvaranje NO u endotelnim ćelijama na dozno i vremenski zavisan način (0,1-0,6 μM do 30 minuta). Ovo stvaranje NO u HUVEC i TrHBMEC izazvano bradikininom blokirano je od strane konkurentskih inhibitora NO-sintaze (NOS), pokazujući NOS-zavisnost. Utvrdili smo da bradikinin značajno smanjuje stvaranje iRNK endotelne forme NOS (eNOS), kao i odnos eNOS i β-aktina u HUVEC (dvostruko manje). Pored toga, bradikinin ne povećava ekspresiju iRNK γ-globinskog gena ni u zasebnim progenitorima eritropoeze, niti u zajedničkim kulturama progenitora eritropoeze sa TrHBMEC ili HUVEC posle 24 sata tretmana. Bradikinin ne menja ni odnos γ i β globina u zajedničkim kulturama progenitora eritropoeze sa HUVEC. Zaključak Aktivacija eNO_ izazvana bradikininom dovodi do kratkog i malog povećanja NO u endotelnim ćelijama, što je nedovoljno da podstakne ekspresiju gena za γ-globin. Ovi rezultati naglašavaju važnost povećanog i produženog stvaranja NO radi stimulacije ekspresije γ-globina.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Bradykinin stimulation of nitric oxide production is not sufficient for gamma-globin induction, Stvaranje azot-monoksida izazvano bradikininom nije dovoljno za indukciju gama-globina",
pages = "196-189",
number = "3-4",
volume = "142",
doi = "10.2298/SARH1404189C"
}

Čokić, V., Subotički, T., Beleslin-Čokić, B., Diklić, M., Milenković, P. B.,& Jovčić, G.. (2014). Bradykinin stimulation of nitric oxide production is not sufficient for gamma-globin induction. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(3-4), 189-196.
https://doi.org/10.2298/SARH1404189C

Čokić V, Subotički T, Beleslin-Čokić B, Diklić M, Milenković PB, Jovčić G. Bradykinin stimulation of nitric oxide production is not sufficient for gamma-globin induction. in Srpski arhiv za celokupno lekarstvo. 2014;142(3-4):189-196.
doi:10.2298/SARH1404189C .

Čokić, Vladan, Subotički, Tijana, Beleslin-Čokić, Bojana, Diklić, Miloš, Milenković, Pavle B., Jovčić, Gordana, "Bradykinin stimulation of nitric oxide production is not sufficient for gamma-globin induction" in Srpski arhiv za celokupno lekarstvo, 142, no. 3-4 (2014):189-196,
https://doi.org/10.2298/SARH1404189C . .

TY  - JOUR
AU  - Čokić, Vladan
AU  - Diklić, Miloš
AU  - Subotički, Tijana
AU  - Beleslin-Čokić, Bojana
AU  - Marković, Dragana
AU  - Milenković, Pavle B.
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/591
AB  - Purpose: The purpose of this study was to examine the gene expression profile of granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood (mPB)-derived progenitors, used in transplantation. Methods: We correlated gene expression patterns of highly enriched steady-state peripheral blood (PB)- and mPB-derived CD71(+) cells by microarray and ingenuity pathway analyses, to identify the transcriptional program during in vitro erythroid differentiation. Results: The gene expression was more than doubled in mPB-derived (4180 genes) compared to PB-derived erythroid progenitors (1667 genes) while PB-and mPB-derived erythroid progenitors shared 1534 common genes. Comparative analysis of transcript levels showed differential expression of 54 genes between cultured erythroid progenitors of PB and mPB origin, where we identified common 13 downregulated and 30 upregulated genes. The most significant genes in mPB-derived erythroid progenitors were P4HB, DDIA3, ARPC2 and ATP5G3. Regarding G-CSF stimulation the G-CSF receptor CSF2RB (1.1-fold) was linked via STAT3 to erythroid-specific ALAS2 (2.9-fold) and GATA2 (1.3-fold) factors, all upregulated in mPB-derived erythroid progenitors, coupled to common upregulated NUDC gene involved in the proliferation of erythroid cells. Conclusion: This report provides an extensive transcriptional profile of cultured erythroid progenitors and leads to a better understanding of diversity among the progenitor sources.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood
EP  - 314
IS  - 1
SP  - 304
VL  - 19
UR  - https://hdl.handle.net/21.15107/rcub_rimi_591
ER  - 

@article{
author = "Čokić, Vladan and Diklić, Miloš and Subotički, Tijana and Beleslin-Čokić, Bojana and Marković, Dragana and Milenković, Pavle B. and Jovčić, Gordana",
year = "2014",
abstract = "Purpose: The purpose of this study was to examine the gene expression profile of granulocyte colony stimulating factor (G-CSF)-mobilized peripheral blood (mPB)-derived progenitors, used in transplantation. Methods: We correlated gene expression patterns of highly enriched steady-state peripheral blood (PB)- and mPB-derived CD71(+) cells by microarray and ingenuity pathway analyses, to identify the transcriptional program during in vitro erythroid differentiation. Results: The gene expression was more than doubled in mPB-derived (4180 genes) compared to PB-derived erythroid progenitors (1667 genes) while PB-and mPB-derived erythroid progenitors shared 1534 common genes. Comparative analysis of transcript levels showed differential expression of 54 genes between cultured erythroid progenitors of PB and mPB origin, where we identified common 13 downregulated and 30 upregulated genes. The most significant genes in mPB-derived erythroid progenitors were P4HB, DDIA3, ARPC2 and ATP5G3. Regarding G-CSF stimulation the G-CSF receptor CSF2RB (1.1-fold) was linked via STAT3 to erythroid-specific ALAS2 (2.9-fold) and GATA2 (1.3-fold) factors, all upregulated in mPB-derived erythroid progenitors, coupled to common upregulated NUDC gene involved in the proliferation of erythroid cells. Conclusion: This report provides an extensive transcriptional profile of cultured erythroid progenitors and leads to a better understanding of diversity among the progenitor sources.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood",
pages = "314-304",
number = "1",
volume = "19",
url = "https://hdl.handle.net/21.15107/rcub_rimi_591"
}

Čokić, V., Diklić, M., Subotički, T., Beleslin-Čokić, B., Marković, D., Milenković, P. B.,& Jovčić, G.. (2014). Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 19(1), 304-314.
https://hdl.handle.net/21.15107/rcub_rimi_591

Čokić V, Diklić M, Subotički T, Beleslin-Čokić B, Marković D, Milenković PB, Jovčić G. Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood. in Journal of BUON. 2014;19(1):304-314.
https://hdl.handle.net/21.15107/rcub_rimi_591 .

Čokić, Vladan, Diklić, Miloš, Subotički, Tijana, Beleslin-Čokić, Bojana, Marković, Dragana, Milenković, Pavle B., Jovčić, Gordana, "Transcriptional profiling of erythroid progenitors from G-CSF mobilized and nonmobilized peripheral blood" in Journal of BUON, 19, no. 1 (2014):304-314,
https://hdl.handle.net/21.15107/rcub_rimi_591 .

TY  - CONF
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Marković, Dragana
AU  - Mitrović, Olivera
AU  - Mojsilović, S.
AU  - Stošić-Grujičić, Stanislava
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/564
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress
EP  - 179
SP  - 179
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimi_564
ER  - 

@conference{
author = "Budeč, Mirela and Vignjević, Sanja and Marković, Dragana and Mitrović, Olivera and Mojsilović, S. and Stošić-Grujičić, Stanislava and Čokić, Vladan and Jovčić, Gordana",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress",
pages = "179-179",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_rimi_564"
}

Budeč, M., Vignjević, S., Marković, D., Mitrović, O., Mojsilović, S., Stošić-Grujičić, S., Čokić, V.,& Jovčić, G.. (2014). Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 179-179.
https://hdl.handle.net/21.15107/rcub_rimi_564

Budeč M, Vignjević S, Marković D, Mitrović O, Mojsilović S, Stošić-Grujičić S, Čokić V, Jovčić G. Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress. in Haematologica. 2014;99:179-179.
https://hdl.handle.net/21.15107/rcub_rimi_564 .

Budeč, Mirela, Vignjević, Sanja, Marković, Dragana, Mitrović, Olivera, Mojsilović, S., Stošić-Grujičić, Stanislava, Čokić, Vladan, Jovčić, Gordana, "Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress" in Haematologica, 99 (2014):179-179,
https://hdl.handle.net/21.15107/rcub_rimi_564 .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Jauković, Aleksandra
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/599
AB  - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Mesenchymal stem cells isolated from human periodontal ligament
EP  - 271
IS  - 1
SP  - 261
VL  - 66
DO  - 10.2298/ABS1401261M
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okić Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cells isolated from human periodontal ligament",
pages = "271-261",
number = "1",
volume = "66",
doi = "10.2298/ABS1401261M"
}

Miletić, M., Mojsilović, S., Okić Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271.
https://doi.org/10.2298/ABS1401261M

Miletić M, Mojsilović S, Okić Đorđević I, Kukolj T, Jauković A, Santibanez JF, Jovčić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271.
doi:10.2298/ABS1401261M .

Miletić, Maja, Mojsilović, S., Okić Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271,
https://doi.org/10.2298/ABS1401261M . .

TY  - CONF
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Marković, Dragana
AU  - Jovčić, Gordana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Čokić, Vladan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/520
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling
EP  - 418
SP  - 418
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimi_520
ER  - 

@conference{
author = "Budeč, Mirela and Vignjević, Sanja and Marković, Dragana and Jovčić, Gordana and Đikić, Dragoslava and Mitrović, Olivera and Vranješ-Đurić, Sanja and Koko, Vesna and Čokić, Vladan",
year = "2013",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling",
pages = "418-418",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_rimi_520"
}

Budeč, M., Vignjević, S., Marković, D., Jovčić, G., Đikić, D., Mitrović, O., Vranješ-Đurić, S., Koko, V.,& Čokić, V.. (2013). Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling. in Haematologica
Ferrata Storti Foundation, Pavia., 98, 418-418.
https://hdl.handle.net/21.15107/rcub_rimi_520

Budeč M, Vignjević S, Marković D, Jovčić G, Đikić D, Mitrović O, Vranješ-Đurić S, Koko V, Čokić V. Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling. in Haematologica. 2013;98:418-418.
https://hdl.handle.net/21.15107/rcub_rimi_520 .

Budeč, Mirela, Vignjević, Sanja, Marković, Dragana, Jovčić, Gordana, Đikić, Dragoslava, Mitrović, Olivera, Vranješ-Đurić, Sanja, Koko, Vesna, Čokić, Vladan, "Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling" in Haematologica, 98 (2013):418-418,
https://hdl.handle.net/21.15107/rcub_rimi_520 .

TY  - CONF
AU  - Diklić, Miloš
AU  - Subotički, Tijana
AU  - Beleslin-Čokić, Bojana
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/524
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Transcriptional profiling of human erythroid progenitors from g-csf mobilized and nonmobilized peripheral blood
EP  - 189
SP  - 189
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimi_524
ER  - 

@conference{
author = "Diklić, Miloš and Subotički, Tijana and Beleslin-Čokić, Bojana and Jovčić, Gordana and Čokić, Vladan",
year = "2013",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Transcriptional profiling of human erythroid progenitors from g-csf mobilized and nonmobilized peripheral blood",
pages = "189-189",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_rimi_524"
}

Diklić, M., Subotički, T., Beleslin-Čokić, B., Jovčić, G.,& Čokić, V.. (2013). Transcriptional profiling of human erythroid progenitors from g-csf mobilized and nonmobilized peripheral blood. in Haematologica
Ferrata Storti Foundation, Pavia., 98, 189-189.
https://hdl.handle.net/21.15107/rcub_rimi_524

Diklić M, Subotički T, Beleslin-Čokić B, Jovčić G, Čokić V. Transcriptional profiling of human erythroid progenitors from g-csf mobilized and nonmobilized peripheral blood. in Haematologica. 2013;98:189-189.
https://hdl.handle.net/21.15107/rcub_rimi_524 .

Diklić, Miloš, Subotički, Tijana, Beleslin-Čokić, Bojana, Jovčić, Gordana, Čokić, Vladan, "Transcriptional profiling of human erythroid progenitors from g-csf mobilized and nonmobilized peripheral blood" in Haematologica, 98 (2013):189-189,
https://hdl.handle.net/21.15107/rcub_rimi_524 .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Okić Đorđević, Ivana
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/475
AB  - Mesenchymal stem cells (MSCs), beside regenerative potential, possess immunomodulatory properties and their use in managing immune-mediated diseases is intensively studied. We analyzed the effects of MSCs isolated from human adipose tissue (AT-MSCs), dental pulp (DP-MSCs), peripheral blood (PB-MSCs) and umbilical cord Wharton's jelly (UC-MSCs), on the proliferation of allogeneic peripheral blood mononuclear cells (PBMCs). While only AT-MSCs functioned as alloantigen presenting cells, proliferation of PBMCs in response to a phytohemagglutinin (PHA) and alloantigens in mixed lymphocytes reaction (MLR) was inhibited by all MSCs in a cell concentration-dependent manner. Conditioned medium (CM) derived from DP-MSCs, PB-MSCs and UC-MSCs, suppressed the baseline, PHA- and alloantigens-mediated proliferation of PBMC, whereas AT-MSCs-derived CM inhibited MLR, but failed to suppress the spontaneous and PHA-induced PBMCs proliferation. Differences between MSC types were observed in expression of genes related to immunomodulation, including human leukocyte antigens (HLA)-A, HLA-DR, HLA-G5, interleukin 6 (IL)-6, transforming growth factor (TGF)-beta, cyclooxygenase-2 (COX-2) and indoleamine 2,3-dioxygenase (IDO-1), under basal conditions, as well as in response to proinflammatory cytokines, interferon (IFN)-gamma and tumor necrosis factor alpha (TNF)-alpha. While AT-MSCs showed a positive constitutive expression of almost all tested genes that was augmented in response to IFN-gamma and TNF-alpha, only combined cytokine treatment increased HLA-A, COX2 and IL-6 mRNA expression in DP-MSCs and slightly stimulated the expression of HLA-G and TGF-beta in UC-MSCs. Although MSCs from different tissues showed similar potential to suppress proliferation of PBMCs, heterogeneity in the expression of genes related to immunomodulation emphasizes the importance of investigating the role of specific molecular mechanisms in the regulation of immunomodulatory activity of MSCs.
PB  - Termedia Publishing House Ltd, Poznan
T2  - Central European Journal of Immunology
T1  - Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton's jelly
EP  - 429
IS  - 4
SP  - 421
VL  - 38
DO  - 10.5114/ceji.2013.39756
ER  - 

@article{
author = "Trivanović, Drenka and Mojsilović, Slavko and Ilić, Vesna and Krstić, Jelena and Jauković, Aleksandra and Okić Đorđević, Ivana and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2013",
abstract = "Mesenchymal stem cells (MSCs), beside regenerative potential, possess immunomodulatory properties and their use in managing immune-mediated diseases is intensively studied. We analyzed the effects of MSCs isolated from human adipose tissue (AT-MSCs), dental pulp (DP-MSCs), peripheral blood (PB-MSCs) and umbilical cord Wharton's jelly (UC-MSCs), on the proliferation of allogeneic peripheral blood mononuclear cells (PBMCs). While only AT-MSCs functioned as alloantigen presenting cells, proliferation of PBMCs in response to a phytohemagglutinin (PHA) and alloantigens in mixed lymphocytes reaction (MLR) was inhibited by all MSCs in a cell concentration-dependent manner. Conditioned medium (CM) derived from DP-MSCs, PB-MSCs and UC-MSCs, suppressed the baseline, PHA- and alloantigens-mediated proliferation of PBMC, whereas AT-MSCs-derived CM inhibited MLR, but failed to suppress the spontaneous and PHA-induced PBMCs proliferation. Differences between MSC types were observed in expression of genes related to immunomodulation, including human leukocyte antigens (HLA)-A, HLA-DR, HLA-G5, interleukin 6 (IL)-6, transforming growth factor (TGF)-beta, cyclooxygenase-2 (COX-2) and indoleamine 2,3-dioxygenase (IDO-1), under basal conditions, as well as in response to proinflammatory cytokines, interferon (IFN)-gamma and tumor necrosis factor alpha (TNF)-alpha. While AT-MSCs showed a positive constitutive expression of almost all tested genes that was augmented in response to IFN-gamma and TNF-alpha, only combined cytokine treatment increased HLA-A, COX2 and IL-6 mRNA expression in DP-MSCs and slightly stimulated the expression of HLA-G and TGF-beta in UC-MSCs. Although MSCs from different tissues showed similar potential to suppress proliferation of PBMCs, heterogeneity in the expression of genes related to immunomodulation emphasizes the importance of investigating the role of specific molecular mechanisms in the regulation of immunomodulatory activity of MSCs.",
publisher = "Termedia Publishing House Ltd, Poznan",
journal = "Central European Journal of Immunology",
title = "Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton's jelly",
pages = "429-421",
number = "4",
volume = "38",
doi = "10.5114/ceji.2013.39756"
}

Trivanović, D., Mojsilović, S., Ilić, V., Krstić, J., Jauković, A., Okić Đorđević, I., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2013). Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton's jelly. in Central European Journal of Immunology
Termedia Publishing House Ltd, Poznan., 38(4), 421-429.
https://doi.org/10.5114/ceji.2013.39756

Trivanović D, Mojsilović S, Ilić V, Krstić J, Jauković A, Okić Đorđević I, Santibanez JF, Jovčić G, Bugarski D. Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton's jelly. in Central European Journal of Immunology. 2013;38(4):421-429.
doi:10.5114/ceji.2013.39756 .

Trivanović, Drenka, Mojsilović, Slavko, Ilić, Vesna, Krstić, Jelena, Jauković, Aleksandra, Okić Đorđević, Ivana, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Immunomodulatory capacity of human mesenchymal stem cells isolated from adipose tissue, dental pulp, peripheral blood and umbilical cord Wharton's jelly" in Central European Journal of Immunology, 38, no. 4 (2013):421-429,
https://doi.org/10.5114/ceji.2013.39756 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Kocić, Jelena
AU  - Mojsilović, Slavko
AU  - Krstić, Aleksandra
AU  - Ilić, Vesna
AU  - Okić Đorđević, Ivana
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Terzić, Milan
AU  - Bugarski, Diana
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/472
AB  - Introduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton's Jelly (UC-MSCs). Methods. The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. Results. MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and α-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. Conclusion. The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications.
AB  - Uvod. Mezenhimske matične ćelije (MMĆ) su posebno značajne za regenerativnu medicinu. S obzirom na heterogenost njihovih populacija, raznovrsnost izvora i tehnika izolacije, ne postoje jedinstvena obeležja koja određuju MMĆ. Cilj rada. Cilj ove studije bila je uporedna analiza bioloških karakteristika MMĆ izolovanih iz periferne krvi (PK-MMĆ) i Vartonove (Wharton) sluzi pupčane vrpce (VSP-MMĆ). Metode rada. PK-MMĆ i VSP-MMĆ su izolovane na osnovu osobine da prianjaju na plastičnu podlogu, a upoređivane su njihove morfološke odlike, klonogeni i proliferativni kapacitet, imunofenotip i potencijal diferencijacije. Rezultati. Dobijeni rezultati pokazali su da oba tipa MMĆ imaju slične morfološke osobine, sličan proliferativni kapacitet i multipotentni potencijal diferencijacije u ćelije različitih mezenhimskih tkiva (koštanog, masnog, hrskavičavog i mišićnog). Razlike su primećene u klonogenom kapacitetu i imunofenotipu, budući da su VSP-MMĆ ispoljile veći kapacitet za formiranje CFU-F (engl. colony-forming unit-fibroblasts), kao i veću ekspresiju markera tipičnih za embrionalne matične ćelije (Nanog, Sox2, SSEA4). Ispitivanje površinskih antigena tipičnih za MMĆ (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) protočnom citometrijom i imunofluorescentno obeležavanje dodatnih mezenhimskih markera (Vimentin, STRO-1 i alfa-aktina glatkih mišićnih ćelija) nije ukazalo na razlike u ekspresiji ovih markera. Zaključak. Oba tipa MMĆ su pogodan alternativni izvor adultnih MMĆ koje bi se mogle koristiti u ćelijskoj terapiji.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton's jelly
T1  - Osobine mezenhimskih matičnih ćelija izolovanih iz periferne krvi i Vartonove sluzi pupčane vrpce
EP  - 186
IS  - 3-4
SP  - 178
VL  - 141
DO  - 10.2298/SARH1304178T
ER  - 

@article{
author = "Trivanović, Drenka and Kocić, Jelena and Mojsilović, Slavko and Krstić, Aleksandra and Ilić, Vesna and Okić Đorđević, Ivana and Santibanez, Juan F. and Jovčić, Gordana and Terzić, Milan and Bugarski, Diana",
year = "2013",
abstract = "Introduction. Mesenchymal stem cells (MSCs) are a promising tool for regenerative medicine, but due to the heterogeneity of their populations, different sources and isolation techniques, the characteristics defining MSCs are inconsistent. Objective. The aim of this study was to compare the characteristics of MSCs derived from two different human tissues: peripheral blood (PB-MSCs) and umbilical cord Wharton's Jelly (UC-MSCs). Methods. The PB-MSC and UC-MSC were isolated by adherence to plastic after gradient-density separation or an explant culture method, respectively, and compared regarding their morphology, clonogenic efficiency, proliferating rates, immunophenotype and differentiation potential. Results. MSCs derived from both sources exhibit similar morphology, proliferation capacity and multilineage (osteogenic, chondrogenic, adipogenic and myogenic) differentiation potential. Differences were observed in the clonogenic capacity and the immunophenotype, since UC-MSCs showed higher CFU-F (colony-forming units-fibroblastic) cloning efficiency, as well as higher embryonic markers (Nanog, Sox2, SSEA4) expression. When additional surface antigens were analyzed by flow cytometry (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) or immunofluorescent labeling (vimentin, STRO-1 and α-smooth muscle actin), most appeared to have similar epitope profiles irrespective of MSC source. Conclusion. The results obtained demonstrated that both MSCs represent good alternative sources of adult MSCs that could be used in cell therapy applications., Uvod. Mezenhimske matične ćelije (MMĆ) su posebno značajne za regenerativnu medicinu. S obzirom na heterogenost njihovih populacija, raznovrsnost izvora i tehnika izolacije, ne postoje jedinstvena obeležja koja određuju MMĆ. Cilj rada. Cilj ove studije bila je uporedna analiza bioloških karakteristika MMĆ izolovanih iz periferne krvi (PK-MMĆ) i Vartonove (Wharton) sluzi pupčane vrpce (VSP-MMĆ). Metode rada. PK-MMĆ i VSP-MMĆ su izolovane na osnovu osobine da prianjaju na plastičnu podlogu, a upoređivane su njihove morfološke odlike, klonogeni i proliferativni kapacitet, imunofenotip i potencijal diferencijacije. Rezultati. Dobijeni rezultati pokazali su da oba tipa MMĆ imaju slične morfološke osobine, sličan proliferativni kapacitet i multipotentni potencijal diferencijacije u ćelije različitih mezenhimskih tkiva (koštanog, masnog, hrskavičavog i mišićnog). Razlike su primećene u klonogenom kapacitetu i imunofenotipu, budući da su VSP-MMĆ ispoljile veći kapacitet za formiranje CFU-F (engl. colony-forming unit-fibroblasts), kao i veću ekspresiju markera tipičnih za embrionalne matične ćelije (Nanog, Sox2, SSEA4). Ispitivanje površinskih antigena tipičnih za MMĆ (CD44, CD90, CD105, CD33, CD34, CD45, CD11b, CD235a) protočnom citometrijom i imunofluorescentno obeležavanje dodatnih mezenhimskih markera (Vimentin, STRO-1 i alfa-aktina glatkih mišićnih ćelija) nije ukazalo na razlike u ekspresiji ovih markera. Zaključak. Oba tipa MMĆ su pogodan alternativni izvor adultnih MMĆ koje bi se mogle koristiti u ćelijskoj terapiji.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton's jelly, Osobine mezenhimskih matičnih ćelija izolovanih iz periferne krvi i Vartonove sluzi pupčane vrpce",
pages = "186-178",
number = "3-4",
volume = "141",
doi = "10.2298/SARH1304178T"
}

Trivanović, D., Kocić, J., Mojsilović, S., Krstić, A., Ilić, V., Okić Đorđević, I., Santibanez, J. F., Jovčić, G., Terzić, M.,& Bugarski, D.. (2013). Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton's jelly. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 141(3-4), 178-186.
https://doi.org/10.2298/SARH1304178T

Trivanović D, Kocić J, Mojsilović S, Krstić A, Ilić V, Okić Đorđević I, Santibanez JF, Jovčić G, Terzić M, Bugarski D. Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton's jelly. in Srpski arhiv za celokupno lekarstvo. 2013;141(3-4):178-186.
doi:10.2298/SARH1304178T .

Trivanović, Drenka, Kocić, Jelena, Mojsilović, Slavko, Krstić, Aleksandra, Ilić, Vesna, Okić Đorđević, Ivana, Santibanez, Juan F., Jovčić, Gordana, Terzić, Milan, Bugarski, Diana, "Mesenchymal stem cells isolated from peripheral blood and umbilical cord Wharton's jelly" in Srpski arhiv za celokupno lekarstvo, 141, no. 3-4 (2013):178-186,
https://doi.org/10.2298/SARH1304178T . .

TY  - CONF
AU  - Mitrović, Olivera
AU  - Mossuz, Pascal
AU  - Diklić, Miloš
AU  - Sefer, Dijana
AU  - Ilić, B.
AU  - Peruničić, Maja
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/518
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Expression analysis of jak-stat dependent s100 calcium binding proteins a4 and a12 in myeloproliferative neoplasms
EP  - 616
SP  - 616
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimi_518
ER  - 

@conference{
author = "Mitrović, Olivera and Mossuz, Pascal and Diklić, Miloš and Sefer, Dijana and Ilić, B. and Peruničić, Maja and Jovčić, Gordana and Čokić, Vladan",
year = "2013",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Expression analysis of jak-stat dependent s100 calcium binding proteins a4 and a12 in myeloproliferative neoplasms",
pages = "616-616",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_rimi_518"
}

Mitrović, O., Mossuz, P., Diklić, M., Sefer, D., Ilić, B., Peruničić, M., Jovčić, G.,& Čokić, V.. (2013). Expression analysis of jak-stat dependent s100 calcium binding proteins a4 and a12 in myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 98, 616-616.
https://hdl.handle.net/21.15107/rcub_rimi_518

Mitrović O, Mossuz P, Diklić M, Sefer D, Ilić B, Peruničić M, Jovčić G, Čokić V. Expression analysis of jak-stat dependent s100 calcium binding proteins a4 and a12 in myeloproliferative neoplasms. in Haematologica. 2013;98:616-616.
https://hdl.handle.net/21.15107/rcub_rimi_518 .

Mitrović, Olivera, Mossuz, Pascal, Diklić, Miloš, Sefer, Dijana, Ilić, B., Peruničić, Maja, Jovčić, Gordana, Čokić, Vladan, "Expression analysis of jak-stat dependent s100 calcium binding proteins a4 and a12 in myeloproliferative neoplasms" in Haematologica, 98 (2013):616-616,
https://hdl.handle.net/21.15107/rcub_rimi_518 .

TY  - CONF
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Đikić, Dragoslava
AU  - Jovčić, Gordana
AU  - Marković, Dragana
AU  - Mitrović, Olivera
AU  - Diklić, Miloš
AU  - Breković, Tijana
AU  - Čokić, Vladan
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/435
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Glucocorticoid receptor selectively mediates the expansion of erythroid progenitor cells during chronic restraint stress
EP  - 389
SP  - 389
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_rimi_435
ER  - 

@conference{
author = "Vignjević, Sanja and Budeč, Mirela and Đikić, Dragoslava and Jovčić, Gordana and Marković, Dragana and Mitrović, Olivera and Diklić, Miloš and Breković, Tijana and Čokić, Vladan",
year = "2012",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Glucocorticoid receptor selectively mediates the expansion of erythroid progenitor cells during chronic restraint stress",
pages = "389-389",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_rimi_435"
}

Vignjević, S., Budeč, M., Đikić, D., Jovčić, G., Marković, D., Mitrović, O., Diklić, M., Breković, T.,& Čokić, V.. (2012). Glucocorticoid receptor selectively mediates the expansion of erythroid progenitor cells during chronic restraint stress. in Haematologica
Ferrata Storti Foundation, Pavia., 97, 389-389.
https://hdl.handle.net/21.15107/rcub_rimi_435

Vignjević S, Budeč M, Đikić D, Jovčić G, Marković D, Mitrović O, Diklić M, Breković T, Čokić V. Glucocorticoid receptor selectively mediates the expansion of erythroid progenitor cells during chronic restraint stress. in Haematologica. 2012;97:389-389.
https://hdl.handle.net/21.15107/rcub_rimi_435 .

Vignjević, Sanja, Budeč, Mirela, Đikić, Dragoslava, Jovčić, Gordana, Marković, Dragana, Mitrović, Olivera, Diklić, Miloš, Breković, Tijana, Čokić, Vladan, "Glucocorticoid receptor selectively mediates the expansion of erythroid progenitor cells during chronic restraint stress" in Haematologica, 97 (2012):389-389,
https://hdl.handle.net/21.15107/rcub_rimi_435 .

TY  - JOUR
AU  - Krstić, Aleksandra
AU  - Kocić, Jelena
AU  - Ilić, Vesna
AU  - Mojsilović, S.
AU  - Okić Đorđević, Ivana
AU  - Trivanović, Drenka
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/403
AB  - Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in murine bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors.
PB  - Biolife Sas, Silva Marina (Te)
T2  - Journal of Biological Regulators & Homeostatic Agents
T1  - Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors
EP  - 652
IS  - 4
SP  - 641
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimi_403
ER  - 

@article{
author = "Krstić, Aleksandra and Kocić, Jelena and Ilić, Vesna and Mojsilović, S. and Okić Đorđević, Ivana and Trivanović, Drenka and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2012",
abstract = "Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in murine bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors.",
publisher = "Biolife Sas, Silva Marina (Te)",
journal = "Journal of Biological Regulators & Homeostatic Agents",
title = "Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors",
pages = "652-641",
number = "4",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimi_403"
}

Krstić, A., Kocić, J., Ilić, V., Mojsilović, S., Okić Đorđević, I., Trivanović, D., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2012). Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors. in Journal of Biological Regulators & Homeostatic Agents
Biolife Sas, Silva Marina (Te)., 26(4), 641-652.
https://hdl.handle.net/21.15107/rcub_rimi_403

Krstić A, Kocić J, Ilić V, Mojsilović S, Okić Đorđević I, Trivanović D, Santibanez JF, Jovčić G, Bugarski D. Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors. in Journal of Biological Regulators & Homeostatic Agents. 2012;26(4):641-652.
https://hdl.handle.net/21.15107/rcub_rimi_403 .

Krstić, Aleksandra, Kocić, Jelena, Ilić, Vesna, Mojsilović, S., Okić Đorđević, Ivana, Trivanović, Drenka, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors" in Journal of Biological Regulators & Homeostatic Agents, 26, no. 4 (2012):641-652,
https://hdl.handle.net/21.15107/rcub_rimi_403 .

TY  - JOUR
AU  - Krstić, Aleksandra
AU  - Mojsilović, Slavko
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/392
AB  - It has long been known that T cells have the potential to modulate hematopoietic response in different ways. More recently, the importance of interleukin (IL)-17-secreting Th17 cells in T-cell-mediated regulation of hematopoiesis was indicated by the line of evidence that IL-17 links T-cell function and hematopoiesis through stimulation of granulopoiesis and neutrophil trafficking. Furthermore, our data demonstrated that IL-17 also affects other cells of hematopoietic system, such as erythroid progenitors, as well as mesenchymal stem cells. In order to better understand the regulatory role of IL-17 in hematopoiesis, molecular mechanisms underlying the effects of IL-17 on hematopoietic and mesenchymal stem cells were also studied.
PB  - Humana Press Inc, Totowa
T2  - Immunologic Research
T1  - The potential of interleukin-17 to mediate hematopoietic response
EP  - 41
IS  - 1-2
SP  - 34
VL  - 52
DO  - 10.1007/s12026-012-8276-8
ER  - 

@article{
author = "Krstić, Aleksandra and Mojsilović, Slavko and Jovčić, Gordana and Bugarski, Diana",
year = "2012",
abstract = "It has long been known that T cells have the potential to modulate hematopoietic response in different ways. More recently, the importance of interleukin (IL)-17-secreting Th17 cells in T-cell-mediated regulation of hematopoiesis was indicated by the line of evidence that IL-17 links T-cell function and hematopoiesis through stimulation of granulopoiesis and neutrophil trafficking. Furthermore, our data demonstrated that IL-17 also affects other cells of hematopoietic system, such as erythroid progenitors, as well as mesenchymal stem cells. In order to better understand the regulatory role of IL-17 in hematopoiesis, molecular mechanisms underlying the effects of IL-17 on hematopoietic and mesenchymal stem cells were also studied.",
publisher = "Humana Press Inc, Totowa",
journal = "Immunologic Research",
title = "The potential of interleukin-17 to mediate hematopoietic response",
pages = "41-34",
number = "1-2",
volume = "52",
doi = "10.1007/s12026-012-8276-8"
}

Krstić, A., Mojsilović, S., Jovčić, G.,& Bugarski, D.. (2012). The potential of interleukin-17 to mediate hematopoietic response. in Immunologic Research
Humana Press Inc, Totowa., 52(1-2), 34-41.
https://doi.org/10.1007/s12026-012-8276-8

Krstić A, Mojsilović S, Jovčić G, Bugarski D. The potential of interleukin-17 to mediate hematopoietic response. in Immunologic Research. 2012;52(1-2):34-41.
doi:10.1007/s12026-012-8276-8 .

Krstić, Aleksandra, Mojsilović, Slavko, Jovčić, Gordana, Bugarski, Diana, "The potential of interleukin-17 to mediate hematopoietic response" in Immunologic Research, 52, no. 1-2 (2012):34-41,
https://doi.org/10.1007/s12026-012-8276-8 . .

TY  - CONF
AU  - Mitrović, Olivera
AU  - Marković, Dragana
AU  - Beleslin-Čokić, Bojana
AU  - Đikić, Dragoslava
AU  - Peruničić, Maja
AU  - Leković, Danijela
AU  - Jaković, L.
AU  - Budeč, Mirela
AU  - Jovčić, Gordana
AU  - Gotić, Mirjana
AU  - Puri, Raj K.
AU  - Čokić, Vladan
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/434
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Angiogenic factors expression in bone marrow and peripheral blood of myeloproliferative neoplasms
EP  - 628
SP  - 628
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_rimi_434
ER  - 

@conference{
author = "Mitrović, Olivera and Marković, Dragana and Beleslin-Čokić, Bojana and Đikić, Dragoslava and Peruničić, Maja and Leković, Danijela and Jaković, L. and Budeč, Mirela and Jovčić, Gordana and Gotić, Mirjana and Puri, Raj K. and Čokić, Vladan",
year = "2012",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Angiogenic factors expression in bone marrow and peripheral blood of myeloproliferative neoplasms",
pages = "628-628",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_rimi_434"
}

Mitrović, O., Marković, D., Beleslin-Čokić, B., Đikić, D., Peruničić, M., Leković, D., Jaković, L., Budeč, M., Jovčić, G., Gotić, M., Puri, R. K.,& Čokić, V.. (2012). Angiogenic factors expression in bone marrow and peripheral blood of myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 97, 628-628.
https://hdl.handle.net/21.15107/rcub_rimi_434

Mitrović O, Marković D, Beleslin-Čokić B, Đikić D, Peruničić M, Leković D, Jaković L, Budeč M, Jovčić G, Gotić M, Puri RK, Čokić V. Angiogenic factors expression in bone marrow and peripheral blood of myeloproliferative neoplasms. in Haematologica. 2012;97:628-628.
https://hdl.handle.net/21.15107/rcub_rimi_434 .

Mitrović, Olivera, Marković, Dragana, Beleslin-Čokić, Bojana, Đikić, Dragoslava, Peruničić, Maja, Leković, Danijela, Jaković, L., Budeč, Mirela, Jovčić, Gordana, Gotić, Mirjana, Puri, Raj K., Čokić, Vladan, "Angiogenic factors expression in bone marrow and peripheral blood of myeloproliferative neoplasms" in Haematologica, 97 (2012):628-628,
https://hdl.handle.net/21.15107/rcub_rimi_434 .

TY  - JOUR
AU  - Mojsilović, Slavko
AU  - Krstić, Aleksandra
AU  - Ilić, Vesna
AU  - Okić Đorđević, Ivana
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2011
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/353
AB  - The mouse is a suitable experimental model to study the biology of mesenchymal stem cells (MSCs), as well as to be used in biocompatibility studies and tissue engineering models. However, the isolation and purification of murine MSCs is far more challenging than their counterparts from other species. In this study, we isolated, expanded and characterized mouse MSCs from bone marrow (BM-MSCs). Additionally, we analyzed the effects of two regulatory molecules, interleukin 17 (IL-17) and basic fibroblast growth factor (bFGF), on BM-MSCs growth and elucidated the signaling pathways involved. The results revealed that IL-17 increased the frequency of colony-forming units fibroblast (CFU-F) as well as the BM-MSCs proliferation in a dose-dependent manner, while bFGF supplementation had no significant effect on CFU-F frequency but induced an increase in cell proliferation. Their combined usage did not produce additive effects on BM-MSCs proliferation and even induced reduction in the number of CFU-F. Also, the involvement of both p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases (MAPKs) signaling in proliferative activity of IL-17 and bFGF on murine BM-MSCs and, moreover, the increased co-activation of a common signaling molecule, p38 MAPK, were demonstrated. Together, the data presented highlighted the role of IL-17 and bFGF in murine BM-MSCs proliferation and pointed to the complexity and specificity of the signaling networks leading to MSCs proliferation in response to different regulatory molecules.
PB  - Springer, New York
T2  - Cell & Tissue Research
T1  - IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation
EP  - 316
IS  - 3
SP  - 305
VL  - 346
DO  - 10.1007/s00441-011-1284-5
ER  - 

@article{
author = "Mojsilović, Slavko and Krstić, Aleksandra and Ilić, Vesna and Okić Đorđević, Ivana and Krstić, Jelena and Trivanović, Drenka and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2011",
abstract = "The mouse is a suitable experimental model to study the biology of mesenchymal stem cells (MSCs), as well as to be used in biocompatibility studies and tissue engineering models. However, the isolation and purification of murine MSCs is far more challenging than their counterparts from other species. In this study, we isolated, expanded and characterized mouse MSCs from bone marrow (BM-MSCs). Additionally, we analyzed the effects of two regulatory molecules, interleukin 17 (IL-17) and basic fibroblast growth factor (bFGF), on BM-MSCs growth and elucidated the signaling pathways involved. The results revealed that IL-17 increased the frequency of colony-forming units fibroblast (CFU-F) as well as the BM-MSCs proliferation in a dose-dependent manner, while bFGF supplementation had no significant effect on CFU-F frequency but induced an increase in cell proliferation. Their combined usage did not produce additive effects on BM-MSCs proliferation and even induced reduction in the number of CFU-F. Also, the involvement of both p38 and extracellular signal-regulated kinase (ERK) mitogen-activated protein kinases (MAPKs) signaling in proliferative activity of IL-17 and bFGF on murine BM-MSCs and, moreover, the increased co-activation of a common signaling molecule, p38 MAPK, were demonstrated. Together, the data presented highlighted the role of IL-17 and bFGF in murine BM-MSCs proliferation and pointed to the complexity and specificity of the signaling networks leading to MSCs proliferation in response to different regulatory molecules.",
publisher = "Springer, New York",
journal = "Cell & Tissue Research",
title = "IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation",
pages = "316-305",
number = "3",
volume = "346",
doi = "10.1007/s00441-011-1284-5"
}

Mojsilović, S., Krstić, A., Ilić, V., Okić Đorđević, I., Krstić, J., Trivanović, D., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2011). IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation. in Cell & Tissue Research
Springer, New York., 346(3), 305-316.
https://doi.org/10.1007/s00441-011-1284-5

Mojsilović S, Krstić A, Ilić V, Okić Đorđević I, Krstić J, Trivanović D, Santibanez JF, Jovčić G, Bugarski D. IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation. in Cell & Tissue Research. 2011;346(3):305-316.
doi:10.1007/s00441-011-1284-5 .

Mojsilović, Slavko, Krstić, Aleksandra, Ilić, Vesna, Okić Đorđević, Ivana, Krstić, Jelena, Trivanović, Drenka, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "IL-17 and FGF signaling involved in mouse mesenchymal stem cell proliferation" in Cell & Tissue Research, 346, no. 3 (2011):305-316,
https://doi.org/10.1007/s00441-011-1284-5 . .

TY  - JOUR
AU  - Nikolić, N.
AU  - Krstić, Aleksandra
AU  - Trivanović, Drenka
AU  - Mojsilović, S.
AU  - Kocić, Jelena
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2011
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/350
AB  - In the present study we have isolated and identified mesenchymal stem cells (MSCs) from the exfoliated deciduous teeth dental pulp (DP-MSCs), as plastic-adherent, spindle-shaped cells with a high proliferative potential. Immunophenotype analyses revealed that DP-MSCs were positive for mesenchymal cell markers (CD90, CD44, CD105, STRO-1, vimentin and α-SMA), and negative for hematopoietic stem cell markers (CD11b, CD33, CD34, CD45, CD235a). DPMSCs were also capable of differentiating into adipogenic, chondrogenic, myogenic and osteogenic lineages, fulfilling the functional criterion for their characterization. These results demonstrate that DP-MSCs offer a valuable, readily accessible source to obtain and store adult stem cells for future use.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Mesenchymal stem cell properties of dental pulp cells from deciduous teeth
EP  - 942
IS  - 4
SP  - 933
VL  - 63
DO  - 10.2298/ABS1104933N
ER  - 

@article{
author = "Nikolić, N. and Krstić, Aleksandra and Trivanović, Drenka and Mojsilović, S. and Kocić, Jelena and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2011",
abstract = "In the present study we have isolated and identified mesenchymal stem cells (MSCs) from the exfoliated deciduous teeth dental pulp (DP-MSCs), as plastic-adherent, spindle-shaped cells with a high proliferative potential. Immunophenotype analyses revealed that DP-MSCs were positive for mesenchymal cell markers (CD90, CD44, CD105, STRO-1, vimentin and α-SMA), and negative for hematopoietic stem cell markers (CD11b, CD33, CD34, CD45, CD235a). DPMSCs were also capable of differentiating into adipogenic, chondrogenic, myogenic and osteogenic lineages, fulfilling the functional criterion for their characterization. These results demonstrate that DP-MSCs offer a valuable, readily accessible source to obtain and store adult stem cells for future use.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cell properties of dental pulp cells from deciduous teeth",
pages = "942-933",
number = "4",
volume = "63",
doi = "10.2298/ABS1104933N"
}

Nikolić, N., Krstić, A., Trivanović, D., Mojsilović, S., Kocić, J., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2011). Mesenchymal stem cell properties of dental pulp cells from deciduous teeth. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(4), 933-942.
https://doi.org/10.2298/ABS1104933N

Nikolić N, Krstić A, Trivanović D, Mojsilović S, Kocić J, Santibanez JF, Jovčić G, Bugarski D. Mesenchymal stem cell properties of dental pulp cells from deciduous teeth. in Archives of Biological Sciences. 2011;63(4):933-942.
doi:10.2298/ABS1104933N .

Nikolić, N., Krstić, Aleksandra, Trivanović, Drenka, Mojsilović, S., Kocić, Jelena, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cell properties of dental pulp cells from deciduous teeth" in Archives of Biological Sciences, 63, no. 4 (2011):933-942,
https://doi.org/10.2298/ABS1104933N . .