TY  - CONF
AU  - Subotički, Tijana
AU  - Beleslin-Čokić, Bojana
AU  - Đikić, Dragoslava
AU  - Mojsilović, S.
AU  - Sefer, Dijana
AU  - Bjelica, Sunčica
AU  - Santibanez, Juan F.
AU  - Čokić, Vladan
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/814
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - The inhibition of jak/stat signaling is compensated by activation of mapk pathway in myeloproliferative neoplasms
EP  - 537
SP  - 536
VL  - 102
UR  - https://hdl.handle.net/21.15107/rcub_rimi_814
ER  - 

@conference{
author = "Subotički, Tijana and Beleslin-Čokić, Bojana and Đikić, Dragoslava and Mojsilović, S. and Sefer, Dijana and Bjelica, Sunčica and Santibanez, Juan F. and Čokić, Vladan",
year = "2017",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "The inhibition of jak/stat signaling is compensated by activation of mapk pathway in myeloproliferative neoplasms",
pages = "537-536",
volume = "102",
url = "https://hdl.handle.net/21.15107/rcub_rimi_814"
}

Subotički, T., Beleslin-Čokić, B., Đikić, D., Mojsilović, S., Sefer, D., Bjelica, S., Santibanez, J. F.,& Čokić, V.. (2017). The inhibition of jak/stat signaling is compensated by activation of mapk pathway in myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 102, 536-537.
https://hdl.handle.net/21.15107/rcub_rimi_814

Subotički T, Beleslin-Čokić B, Đikić D, Mojsilović S, Sefer D, Bjelica S, Santibanez JF, Čokić V. The inhibition of jak/stat signaling is compensated by activation of mapk pathway in myeloproliferative neoplasms. in Haematologica. 2017;102:536-537.
https://hdl.handle.net/21.15107/rcub_rimi_814 .

Subotički, Tijana, Beleslin-Čokić, Bojana, Đikić, Dragoslava, Mojsilović, S., Sefer, Dijana, Bjelica, Sunčica, Santibanez, Juan F., Čokić, Vladan, "The inhibition of jak/stat signaling is compensated by activation of mapk pathway in myeloproliferative neoplasms" in Haematologica, 102 (2017):536-537,
https://hdl.handle.net/21.15107/rcub_rimi_814 .

TY  - CONF
AU  - Subotički, Tijana
AU  - Beleslin-Čokić, Bojana
AU  - Leković, Danijela
AU  - Mojsilović, S.
AU  - Mitrović-Ajtić, Olivera
AU  - Kovačić, Marijana
AU  - Diklić, Miloš
AU  - Gotić, Mirjana
AU  - Čokić, Vladan
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/817
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Proinflammatory cytokine il-6 stimulation of angiogenic factors and DNA replication is blocked by jak-stat pathway inhibition in myeloproliferative neoplasms
EP  - 541
SP  - 540
VL  - 102
UR  - https://hdl.handle.net/21.15107/rcub_rimi_817
ER  - 

@conference{
author = "Subotički, Tijana and Beleslin-Čokić, Bojana and Leković, Danijela and Mojsilović, S. and Mitrović-Ajtić, Olivera and Kovačić, Marijana and Diklić, Miloš and Gotić, Mirjana and Čokić, Vladan",
year = "2017",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Proinflammatory cytokine il-6 stimulation of angiogenic factors and DNA replication is blocked by jak-stat pathway inhibition in myeloproliferative neoplasms",
pages = "541-540",
volume = "102",
url = "https://hdl.handle.net/21.15107/rcub_rimi_817"
}

Subotički, T., Beleslin-Čokić, B., Leković, D., Mojsilović, S., Mitrović-Ajtić, O., Kovačić, M., Diklić, M., Gotić, M.,& Čokić, V.. (2017). Proinflammatory cytokine il-6 stimulation of angiogenic factors and DNA replication is blocked by jak-stat pathway inhibition in myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 102, 540-541.
https://hdl.handle.net/21.15107/rcub_rimi_817

Subotički T, Beleslin-Čokić B, Leković D, Mojsilović S, Mitrović-Ajtić O, Kovačić M, Diklić M, Gotić M, Čokić V. Proinflammatory cytokine il-6 stimulation of angiogenic factors and DNA replication is blocked by jak-stat pathway inhibition in myeloproliferative neoplasms. in Haematologica. 2017;102:540-541.
https://hdl.handle.net/21.15107/rcub_rimi_817 .

Subotički, Tijana, Beleslin-Čokić, Bojana, Leković, Danijela, Mojsilović, S., Mitrović-Ajtić, Olivera, Kovačić, Marijana, Diklić, Miloš, Gotić, Mirjana, Čokić, Vladan, "Proinflammatory cytokine il-6 stimulation of angiogenic factors and DNA replication is blocked by jak-stat pathway inhibition in myeloproliferative neoplasms" in Haematologica, 102 (2017):540-541,
https://hdl.handle.net/21.15107/rcub_rimi_817 .

TY  - CONF
AU  - Subotički, Tijana
AU  - Mitrović, Olivera
AU  - Diklić, Miloš
AU  - Mićić, Mileva
AU  - Mojsilović, S.
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/557
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis
EP  - 729
SP  - 728
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimi_557
ER  - 

@conference{
author = "Subotički, Tijana and Mitrović, Olivera and Diklić, Miloš and Mićić, Mileva and Mojsilović, S. and Vignjević, Sanja and Budeč, Mirela and Jovčić, Gordana and Čokić, Vladan",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis",
pages = "729-728",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_rimi_557"
}

Subotički, T., Mitrović, O., Diklić, M., Mićić, M., Mojsilović, S., Vignjević, S., Budeč, M., Jovčić, G.,& Čokić, V.. (2014). Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 728-729.
https://hdl.handle.net/21.15107/rcub_rimi_557

Subotički T, Mitrović O, Diklić M, Mićić M, Mojsilović S, Vignjević S, Budeč M, Jovčić G, Čokić V. Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis. in Haematologica. 2014;99:728-729.
https://hdl.handle.net/21.15107/rcub_rimi_557 .

Subotički, Tijana, Mitrović, Olivera, Diklić, Miloš, Mićić, Mileva, Mojsilović, S., Vignjević, Sanja, Budeč, Mirela, Jovčić, Gordana, Čokić, Vladan, "Hydroxycarbamide demonstrates nitric oxide synthase dependence in proliferation and apoptosis" in Haematologica, 99 (2014):728-729,
https://hdl.handle.net/21.15107/rcub_rimi_557 .

TY  - CONF
AU  - Diklić, Miloš
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Milanović, S.
AU  - Mojsilović, S.
AU  - Jovčić, Gordana
AU  - Čokić, Vladan
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/556
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Oxidative stress in myeloproliferative neoplasms
EP  - 662
SP  - 662
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1087
ER  - 

@conference{
author = "Diklić, Miloš and Marković, Dragana and Đikić, Dragoslava and Milanović, S. and Mojsilović, S. and Jovčić, Gordana and Čokić, Vladan",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Oxidative stress in myeloproliferative neoplasms",
pages = "662-662",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1087"
}

Diklić, M., Marković, D., Đikić, D., Milanović, S., Mojsilović, S., Jovčić, G.,& Čokić, V.. (2014). Oxidative stress in myeloproliferative neoplasms. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 662-662.
https://hdl.handle.net/21.15107/rcub_veterinar_1087

Diklić M, Marković D, Đikić D, Milanović S, Mojsilović S, Jovčić G, Čokić V. Oxidative stress in myeloproliferative neoplasms. in Haematologica. 2014;99:662-662.
https://hdl.handle.net/21.15107/rcub_veterinar_1087 .

Diklić, Miloš, Marković, Dragana, Đikić, Dragoslava, Milanović, S., Mojsilović, S., Jovčić, Gordana, Čokić, Vladan, "Oxidative stress in myeloproliferative neoplasms" in Haematologica, 99 (2014):662-662,
https://hdl.handle.net/21.15107/rcub_veterinar_1087 .

TY  - CONF
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Marković, Dragana
AU  - Mitrović, Olivera
AU  - Mojsilović, S.
AU  - Stošić-Grujičić, Stanislava
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/564
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress
EP  - 179
SP  - 179
VL  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimi_564
ER  - 

@conference{
author = "Budeč, Mirela and Vignjević, Sanja and Marković, Dragana and Mitrović, Olivera and Mojsilović, S. and Stošić-Grujičić, Stanislava and Čokić, Vladan and Jovčić, Gordana",
year = "2014",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress",
pages = "179-179",
volume = "99",
url = "https://hdl.handle.net/21.15107/rcub_rimi_564"
}

Budeč, M., Vignjević, S., Marković, D., Mitrović, O., Mojsilović, S., Stošić-Grujičić, S., Čokić, V.,& Jovčić, G.. (2014). Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress. in Haematologica
Ferrata Storti Foundation, Pavia., 99, 179-179.
https://hdl.handle.net/21.15107/rcub_rimi_564

Budeč M, Vignjević S, Marković D, Mitrović O, Mojsilović S, Stošić-Grujičić S, Čokić V, Jovčić G. Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress. in Haematologica. 2014;99:179-179.
https://hdl.handle.net/21.15107/rcub_rimi_564 .

Budeč, Mirela, Vignjević, Sanja, Marković, Dragana, Mitrović, Olivera, Mojsilović, S., Stošić-Grujičić, Stanislava, Čokić, Vladan, Jovčić, Gordana, "Macrophage migration inhibitory factor inhibits overexpansion of immature erythroid cells in the spleen during chronic psychological stress" in Haematologica, 99 (2014):179-179,
https://hdl.handle.net/21.15107/rcub_rimi_564 .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Jauković, Aleksandra
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/599
AB  - Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Mesenchymal stem cells isolated from human periodontal ligament
EP  - 271
IS  - 1
SP  - 261
VL  - 66
DO  - 10.2298/ABS1401261M
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okić Đorđević, Ivana and Kukolj, Tamara and Jauković, Aleksandra and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2014",
abstract = "Mesenchymal stem cells (MSCs) were isolated from human periodontal ligament (hPDL-MSCs) and characterized by their morphology, clonogenic efficiency, proliferation and differentiation capabilities. hPDL-MSCs, derived from normal impacted third molars, possessed all of the properties of MSC, including clonogenic ability, high proliferation rate and multi-lineage (osteogenic, chondrogenic, adipogenic, myogenic) differentiation potential. Moreover, hPDL-MSCs expressed a typical MSC epitope profile, being positive for mesenchymal cell markers (CD44H, CD90, CD105, CD73, CD29, Stro-1, fibronectin, vimentin, alpha-SMA), and negative for hematopoietic stem cell markers (CD34, CD11b, CD45, Glycophorin-CD235a). Additionally, hPDL-MSCs, as primitive and highly multipotent cells, showed high expression of embryonic markers (Nanog, Sox2, SSEA4). The data obtained provided yet further proof that cells with mesenchymal properties can be obtained from periodontal ligament tissue. Although these cells should be further investigated to determine their clinical significance, hPDL-MSCs are believed to provide a renewable and promising cell source for new therapeutic strategies in the treatment of periodontal defects.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cells isolated from human periodontal ligament",
pages = "271-261",
number = "1",
volume = "66",
doi = "10.2298/ABS1401261M"
}

Miletić, M., Mojsilović, S., Okić Đorđević, I., Kukolj, T., Jauković, A., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2014). Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(1), 261-271.
https://doi.org/10.2298/ABS1401261M

Miletić M, Mojsilović S, Okić Đorđević I, Kukolj T, Jauković A, Santibanez JF, Jovčić G, Bugarski D. Mesenchymal stem cells isolated from human periodontal ligament. in Archives of Biological Sciences. 2014;66(1):261-271.
doi:10.2298/ABS1401261M .

Miletić, Maja, Mojsilović, S., Okić Đorđević, Ivana, Kukolj, Tamara, Jauković, Aleksandra, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cells isolated from human periodontal ligament" in Archives of Biological Sciences, 66, no. 1 (2014):261-271,
https://doi.org/10.2298/ABS1401261M . .

TY  - JOUR
AU  - Miletić, Maja
AU  - Mojsilović, S.
AU  - Okić Đorđević, Ivana
AU  - Maletić, Dejan
AU  - Puač, Nevena
AU  - Lazović, Saša
AU  - Malović, Gordana
AU  - Milenković, P.
AU  - Petrović, Zoran Lj.
AU  - Bugarski, Diana
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/487
AB  - Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.
PB  - Iop Publishing Ltd, Bristol
T2  - Journal of Physics D-Applied Physics
T1  - Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells
IS  - 34
VL  - 46
DO  - 10.1088/0022-3727/46/34/345401
ER  - 

@article{
author = "Miletić, Maja and Mojsilović, S. and Okić Đorđević, Ivana and Maletić, Dejan and Puač, Nevena and Lazović, Saša and Malović, Gordana and Milenković, P. and Petrović, Zoran Lj. and Bugarski, Diana",
year = "2013",
abstract = "Here we investigate the influences of non-thermal atmospheric plasma on human mesenchymal stem cells isolated from periodontal ligament (hPDL-MSCs). A specially redesigned plasma needle was used as the source of low-temperature plasma and its effects on different hPDL-MSC functions were investigated. Cell cultures were obtained from extracted normal impacted third molars and characterized for their phenotype and multi-potential differentiation. The hPDL-MSCs possessed all the typical MSC properties, including clonogenic ability, high proliferation rate, specific phenotype and multilineage differentiation. The data regarding the interaction of plasma with hPDL-MSCs demonstrated that plasma treatment inhibited the migration of hPDL-MSCs and induced some detachment, while not affecting their viability. Additionally, plasma significantly attenuated hPDL-MSCs' proliferation, but promoted their osteogenic differentiation. The results of this study indicated that a non-thermal plasma offers specific activity with non-destructive properties that can be advantageous for future dental applications.",
publisher = "Iop Publishing Ltd, Bristol",
journal = "Journal of Physics D-Applied Physics",
title = "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells",
number = "34",
volume = "46",
doi = "10.1088/0022-3727/46/34/345401"
}

Miletić, M., Mojsilović, S., Okić Đorđević, I., Maletić, D., Puač, N., Lazović, S., Malović, G., Milenković, P., Petrović, Z. Lj.,& Bugarski, D.. (2013). Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D-Applied Physics
Iop Publishing Ltd, Bristol., 46(34).
https://doi.org/10.1088/0022-3727/46/34/345401

Miletić M, Mojsilović S, Okić Đorđević I, Maletić D, Puač N, Lazović S, Malović G, Milenković P, Petrović ZL, Bugarski D. Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells. in Journal of Physics D-Applied Physics. 2013;46(34).
doi:10.1088/0022-3727/46/34/345401 .

Miletić, Maja, Mojsilović, S., Okić Đorđević, Ivana, Maletić, Dejan, Puač, Nevena, Lazović, Saša, Malović, Gordana, Milenković, P., Petrović, Zoran Lj., Bugarski, Diana, "Effects of non-thermal atmospheric plasma on human periodontal ligament mesenchymal stem cells" in Journal of Physics D-Applied Physics, 46, no. 34 (2013),
https://doi.org/10.1088/0022-3727/46/34/345401 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Trivanović, Drenka
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Mojsilović, S.
AU  - Santibanez, Juan F.
AU  - Terzić, Milan
AU  - Vesović, D.
AU  - Bugarski, Diana
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/491
AB  - Purpose: Natural products have been investigated for promising new leads in pharmaceutical development. The purpose of this study was to analyze the biological effect of GE132+Natural, a novel supplement consisting of 5 compounds: Resveratrol, Ganoderma lucidum, Sulforaphane, Lycopene and Royal jelly. Methods: The antiproliferative activity of GE132+Natural was tested on 3 different human cancer cell lines: MCF7 (breast cancer cells), PC3 (prostate cancer cells), and SW480 (colon cancer cells), as well as on EA.hy 926 (normal human endothelial cell line). In addition, the cytotoxicity of GE132+Natural on the proliferation of primary human mesenchymal stem cells isolated from dental pulp (DP-MSC), along with its in vitro impact on different peripheral blood parameters, was determined. Results: The results revealed high antiproliferative activity of GE132+Natural on all tested cancer cell lines (PC3, MCF7 and SW480), as well as on the EA.hy 926 endothelial cell line in a dose-dependent manner. However, applied in a wide range of concentrations GE132+Natural did not affect both the proliferation of primary mesenchymal stem cells and the peripheral blood cells counts. Conclusion: The data obtained demonstrated that GE132+Natural is effective in inhibiting cancer cell proliferation, indicating its potential beneficial health effects. In addition, the results pointed that adult mesenchymal stem cells might be valuable as a test system for evaluating the toxicity and efficacy of new medicines or chemicals.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells
EP  - 510
IS  - 2
SP  - 504
VL  - 18
UR  - https://hdl.handle.net/21.15107/rcub_rimi_491
ER  - 

@article{
author = "Okić Đorđević, Ivana and Trivanović, Drenka and Krstić, Jelena and Jauković, Aleksandra and Mojsilović, S. and Santibanez, Juan F. and Terzić, Milan and Vesović, D. and Bugarski, Diana",
year = "2013",
abstract = "Purpose: Natural products have been investigated for promising new leads in pharmaceutical development. The purpose of this study was to analyze the biological effect of GE132+Natural, a novel supplement consisting of 5 compounds: Resveratrol, Ganoderma lucidum, Sulforaphane, Lycopene and Royal jelly. Methods: The antiproliferative activity of GE132+Natural was tested on 3 different human cancer cell lines: MCF7 (breast cancer cells), PC3 (prostate cancer cells), and SW480 (colon cancer cells), as well as on EA.hy 926 (normal human endothelial cell line). In addition, the cytotoxicity of GE132+Natural on the proliferation of primary human mesenchymal stem cells isolated from dental pulp (DP-MSC), along with its in vitro impact on different peripheral blood parameters, was determined. Results: The results revealed high antiproliferative activity of GE132+Natural on all tested cancer cell lines (PC3, MCF7 and SW480), as well as on the EA.hy 926 endothelial cell line in a dose-dependent manner. However, applied in a wide range of concentrations GE132+Natural did not affect both the proliferation of primary mesenchymal stem cells and the peripheral blood cells counts. Conclusion: The data obtained demonstrated that GE132+Natural is effective in inhibiting cancer cell proliferation, indicating its potential beneficial health effects. In addition, the results pointed that adult mesenchymal stem cells might be valuable as a test system for evaluating the toxicity and efficacy of new medicines or chemicals.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells",
pages = "510-504",
number = "2",
volume = "18",
url = "https://hdl.handle.net/21.15107/rcub_rimi_491"
}

Okić Đorđević, I., Trivanović, D., Krstić, J., Jauković, A., Mojsilović, S., Santibanez, J. F., Terzić, M., Vesović, D.,& Bugarski, D.. (2013). GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 18(2), 504-510.
https://hdl.handle.net/21.15107/rcub_rimi_491

Okić Đorđević I, Trivanović D, Krstić J, Jauković A, Mojsilović S, Santibanez JF, Terzić M, Vesović D, Bugarski D. GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells. in Journal of BUON. 2013;18(2):504-510.
https://hdl.handle.net/21.15107/rcub_rimi_491 .

Okić Đorđević, Ivana, Trivanović, Drenka, Krstić, Jelena, Jauković, Aleksandra, Mojsilović, S., Santibanez, Juan F., Terzić, Milan, Vesović, D., Bugarski, Diana, "GE132+Natural: Novel promising dietetic supplement with antiproliferative influence on prostate, colon, and breast cancer cells" in Journal of BUON, 18, no. 2 (2013):504-510,
https://hdl.handle.net/21.15107/rcub_rimi_491 .

TY  - JOUR
AU  - Krstić, Aleksandra
AU  - Kocić, Jelena
AU  - Ilić, Vesna
AU  - Mojsilović, S.
AU  - Okić Đorđević, Ivana
AU  - Trivanović, Drenka
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/403
AB  - Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in murine bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors.
PB  - Biolife Sas, Silva Marina (Te)
T2  - Journal of Biological Regulators & Homeostatic Agents
T1  - Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors
EP  - 652
IS  - 4
SP  - 641
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimi_403
ER  - 

@article{
author = "Krstić, Aleksandra and Kocić, Jelena and Ilić, Vesna and Mojsilović, S. and Okić Đorđević, Ivana and Trivanović, Drenka and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2012",
abstract = "Interleukin-17 is Th17 cell cytokine implicated in regulation of hematopoiesis and inflammation. Besides promoting granulopoiesis, we have previously shown that IL-17 also affects erythropoiesis stimulating the development of early erythroid progenitors, BFU-E, but suppressing, at least partly via p38 MAPK, the growth of late stage erythroid progenitors, CFU-E. The aim of the present study was to investigate the involvement of other MAPKs, JNK and ERK1/2, as well as GATA transcription factors, in IL-17-mediated effects on murine bone marrow erythroid progenitors. Data obtained by use of specific MAPKs inhibitors indicated that MEK1/2-ERK1/2 MAPK signaling mediates IL-17-induced CFU-E inhibition, as well as that JNK and/or MEK1/2-ERK1/2 MAPKs activation underlies IL-17-induced stimulation of BFU-E growth. Furthermore, Western blot analyses demonstrated no effect on early hematopoiesis transcription factor, GATA-2, and enhanced expression level of erythroid-specific factor GATA-1 in murine bone marrow cells after IL-17 stimulation, which in light of previous reports that GATA-1 overexpression inhibits erythroid differentiation, could be related to IL-17-mediated inhibition of CFU-E growth. Although, no contribution for p38, JNK and ERK MAPKs in IL-17-induced GATA-1 expression was shown, data obtained using specific inhibitors pointed to the role of JNK and MEK1/2-ERK1/2 in GATA-1 downregulation. Overall, obtained data gave an insight into the mechanisms by which IL-17 exerts its effects on erythropoiesis, implying the involvement of JNK and ERK MAPKs, as well as GATA-1, in IL-17-regulated growth of erythroid progentors.",
publisher = "Biolife Sas, Silva Marina (Te)",
journal = "Journal of Biological Regulators & Homeostatic Agents",
title = "Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors",
pages = "652-641",
number = "4",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimi_403"
}

Krstić, A., Kocić, J., Ilić, V., Mojsilović, S., Okić Đorđević, I., Trivanović, D., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2012). Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors. in Journal of Biological Regulators & Homeostatic Agents
Biolife Sas, Silva Marina (Te)., 26(4), 641-652.
https://hdl.handle.net/21.15107/rcub_rimi_403

Krstić A, Kocić J, Ilić V, Mojsilović S, Okić Đorđević I, Trivanović D, Santibanez JF, Jovčić G, Bugarski D. Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors. in Journal of Biological Regulators & Homeostatic Agents. 2012;26(4):641-652.
https://hdl.handle.net/21.15107/rcub_rimi_403 .

Krstić, Aleksandra, Kocić, Jelena, Ilić, Vesna, Mojsilović, S., Okić Đorđević, Ivana, Trivanović, Drenka, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Effects of il-17 on erythroid progenitors growth: involvement of mapks and gata transcription factors" in Journal of Biological Regulators & Homeostatic Agents, 26, no. 4 (2012):641-652,
https://hdl.handle.net/21.15107/rcub_rimi_403 .

TY  - CONF
AU  - Maslovarić, Irina
AU  - Mojsilović, S.
AU  - Trivanović, Drenka
AU  - Bugarski, Diana
AU  - Vučić, Vesna M.
AU  - Ilić, Vesna
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/432
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology & Medicine
T1  - Release of reactive oxygen species and production of nitric oxide in the cultures of peripheral blood leukocytes of young athletes
EP  - S184
SP  - S183
VL  - 53
DO  - 10.1016/j.freeradbiomed.2012.08.386
ER  - 

@conference{
author = "Maslovarić, Irina and Mojsilović, S. and Trivanović, Drenka and Bugarski, Diana and Vučić, Vesna M. and Ilić, Vesna",
year = "2012",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology & Medicine",
title = "Release of reactive oxygen species and production of nitric oxide in the cultures of peripheral blood leukocytes of young athletes",
pages = "S184-S183",
volume = "53",
doi = "10.1016/j.freeradbiomed.2012.08.386"
}

Maslovarić, I., Mojsilović, S., Trivanović, D., Bugarski, D., Vučić, V. M.,& Ilić, V.. (2012). Release of reactive oxygen species and production of nitric oxide in the cultures of peripheral blood leukocytes of young athletes. in Free Radical Biology & Medicine
Elsevier Science Inc, New York., 53, S183-S184.
https://doi.org/10.1016/j.freeradbiomed.2012.08.386

Maslovarić I, Mojsilović S, Trivanović D, Bugarski D, Vučić VM, Ilić V. Release of reactive oxygen species and production of nitric oxide in the cultures of peripheral blood leukocytes of young athletes. in Free Radical Biology & Medicine. 2012;53:S183-S184.
doi:10.1016/j.freeradbiomed.2012.08.386 .

Maslovarić, Irina, Mojsilović, S., Trivanović, Drenka, Bugarski, Diana, Vučić, Vesna M., Ilić, Vesna, "Release of reactive oxygen species and production of nitric oxide in the cultures of peripheral blood leukocytes of young athletes" in Free Radical Biology & Medicine, 53 (2012):S183-S184,
https://doi.org/10.1016/j.freeradbiomed.2012.08.386 . .

TY  - JOUR
AU  - Nikolić, N.
AU  - Krstić, Aleksandra
AU  - Trivanović, Drenka
AU  - Mojsilović, S.
AU  - Kocić, Jelena
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2011
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/350
AB  - In the present study we have isolated and identified mesenchymal stem cells (MSCs) from the exfoliated deciduous teeth dental pulp (DP-MSCs), as plastic-adherent, spindle-shaped cells with a high proliferative potential. Immunophenotype analyses revealed that DP-MSCs were positive for mesenchymal cell markers (CD90, CD44, CD105, STRO-1, vimentin and α-SMA), and negative for hematopoietic stem cell markers (CD11b, CD33, CD34, CD45, CD235a). DPMSCs were also capable of differentiating into adipogenic, chondrogenic, myogenic and osteogenic lineages, fulfilling the functional criterion for their characterization. These results demonstrate that DP-MSCs offer a valuable, readily accessible source to obtain and store adult stem cells for future use.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Mesenchymal stem cell properties of dental pulp cells from deciduous teeth
EP  - 942
IS  - 4
SP  - 933
VL  - 63
DO  - 10.2298/ABS1104933N
ER  - 

@article{
author = "Nikolić, N. and Krstić, Aleksandra and Trivanović, Drenka and Mojsilović, S. and Kocić, Jelena and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2011",
abstract = "In the present study we have isolated and identified mesenchymal stem cells (MSCs) from the exfoliated deciduous teeth dental pulp (DP-MSCs), as plastic-adherent, spindle-shaped cells with a high proliferative potential. Immunophenotype analyses revealed that DP-MSCs were positive for mesenchymal cell markers (CD90, CD44, CD105, STRO-1, vimentin and α-SMA), and negative for hematopoietic stem cell markers (CD11b, CD33, CD34, CD45, CD235a). DPMSCs were also capable of differentiating into adipogenic, chondrogenic, myogenic and osteogenic lineages, fulfilling the functional criterion for their characterization. These results demonstrate that DP-MSCs offer a valuable, readily accessible source to obtain and store adult stem cells for future use.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Mesenchymal stem cell properties of dental pulp cells from deciduous teeth",
pages = "942-933",
number = "4",
volume = "63",
doi = "10.2298/ABS1104933N"
}

Nikolić, N., Krstić, A., Trivanović, D., Mojsilović, S., Kocić, J., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2011). Mesenchymal stem cell properties of dental pulp cells from deciduous teeth. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(4), 933-942.
https://doi.org/10.2298/ABS1104933N

Nikolić N, Krstić A, Trivanović D, Mojsilović S, Kocić J, Santibanez JF, Jovčić G, Bugarski D. Mesenchymal stem cell properties of dental pulp cells from deciduous teeth. in Archives of Biological Sciences. 2011;63(4):933-942.
doi:10.2298/ABS1104933N .

Nikolić, N., Krstić, Aleksandra, Trivanović, Drenka, Mojsilović, S., Kocić, Jelena, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Mesenchymal stem cell properties of dental pulp cells from deciduous teeth" in Archives of Biological Sciences, 63, no. 4 (2011):933-942,
https://doi.org/10.2298/ABS1104933N . .

TY  - JOUR
AU  - Krstić, Aleksandra
AU  - Santibanez, Juan F.
AU  - Okić, Ivana
AU  - Mojsilović, S.
AU  - Kocić, Jelena
AU  - Jovčić, Gordana
AU  - Milenković, P.
AU  - Bugarski, Diana
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/266
AB  - Aim: The study was undertaken to extend our investigation concerning both the in vivo activity of interleukin (IL)-17 and the specific role of nitric oxide (NO) in IL-17-induced effects in the process of haematopoiesis. Methods: CBA mice were simultaneously treated with IL-17 and/or nitric oxide synthase (NOS) inhibitor, l-NAME, for 5 days and changes within various haematopoietic cell lineages in bone marrow, spleen and peripheral blood were analysed. Results: Findings showed that administration of both IL-17 and l-NAME stimulated increase in net haematopoiesis in normal mice. IL-17-enhanced myelopoiesis was characterized by stimulation of both femoral and splenic haematopoietic progenitor cells and morphologically recognizable granulocytes. Additionally, IL-17 induced alterations in the frequency of erythroid progenitor cells in both bone marrow and spleen, accompanied with their mobilization to the peripheral blood. As a consequence of these changes in the erythroid cell compartments, significant reticulocytosis was observed, which evidenced that in IL-17-treated mice effective erythropoiesis occurred. Exposure of mice to NOS inhibitor also increased the number of both granulocyte-macrophage and erythroid progenitors in bone marrow and spleens, and these alterations were followed by the mobilization of erythroid progenitors and elevated content of reticulocytes in peripheral blood. The specific role of NO in IL-17-induced haematopoiesis was demonstrated only in the IL-17-reducing effect on bone marrow late stage erythroid progenitors, CFU-E. Conclusion: The results demonstrated the involvement of both IL-17 and NO in the regulation of haematopoietic cell activity in various haematopoietic compartments. They further suggest that IL-17 effects are differentially mediated depending on the haematopoietic microenvironments.
PB  - Wiley, Hoboken
T2  - Acta Physiologica
T1  - Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice
EP  - 41
IS  - 1
SP  - 31
VL  - 199
DO  - 10.1111/j.1748-1716.2010.02082.x
ER  - 

@article{
author = "Krstić, Aleksandra and Santibanez, Juan F. and Okić, Ivana and Mojsilović, S. and Kocić, Jelena and Jovčić, Gordana and Milenković, P. and Bugarski, Diana",
year = "2010",
abstract = "Aim: The study was undertaken to extend our investigation concerning both the in vivo activity of interleukin (IL)-17 and the specific role of nitric oxide (NO) in IL-17-induced effects in the process of haematopoiesis. Methods: CBA mice were simultaneously treated with IL-17 and/or nitric oxide synthase (NOS) inhibitor, l-NAME, for 5 days and changes within various haematopoietic cell lineages in bone marrow, spleen and peripheral blood were analysed. Results: Findings showed that administration of both IL-17 and l-NAME stimulated increase in net haematopoiesis in normal mice. IL-17-enhanced myelopoiesis was characterized by stimulation of both femoral and splenic haematopoietic progenitor cells and morphologically recognizable granulocytes. Additionally, IL-17 induced alterations in the frequency of erythroid progenitor cells in both bone marrow and spleen, accompanied with their mobilization to the peripheral blood. As a consequence of these changes in the erythroid cell compartments, significant reticulocytosis was observed, which evidenced that in IL-17-treated mice effective erythropoiesis occurred. Exposure of mice to NOS inhibitor also increased the number of both granulocyte-macrophage and erythroid progenitors in bone marrow and spleens, and these alterations were followed by the mobilization of erythroid progenitors and elevated content of reticulocytes in peripheral blood. The specific role of NO in IL-17-induced haematopoiesis was demonstrated only in the IL-17-reducing effect on bone marrow late stage erythroid progenitors, CFU-E. Conclusion: The results demonstrated the involvement of both IL-17 and NO in the regulation of haematopoietic cell activity in various haematopoietic compartments. They further suggest that IL-17 effects are differentially mediated depending on the haematopoietic microenvironments.",
publisher = "Wiley, Hoboken",
journal = "Acta Physiologica",
title = "Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice",
pages = "41-31",
number = "1",
volume = "199",
doi = "10.1111/j.1748-1716.2010.02082.x"
}

Krstić, A., Santibanez, J. F., Okić, I., Mojsilović, S., Kocić, J., Jovčić, G., Milenković, P.,& Bugarski, D.. (2010). Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice. in Acta Physiologica
Wiley, Hoboken., 199(1), 31-41.
https://doi.org/10.1111/j.1748-1716.2010.02082.x

Krstić A, Santibanez JF, Okić I, Mojsilović S, Kocić J, Jovčić G, Milenković P, Bugarski D. Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice. in Acta Physiologica. 2010;199(1):31-41.
doi:10.1111/j.1748-1716.2010.02082.x .

Krstić, Aleksandra, Santibanez, Juan F., Okić, Ivana, Mojsilović, S., Kocić, Jelena, Jovčić, Gordana, Milenković, P., Bugarski, Diana, "Combined effect of IL-17 and blockade of nitric oxide biosynthesis on haematopoiesis in mice" in Acta Physiologica, 199, no. 1 (2010):31-41,
https://doi.org/10.1111/j.1748-1716.2010.02082.x . .

TY  - CONF
AU  - Krstić, Aleksandra
AU  - Kocić, Jelena
AU  - Mojsilović, S.
AU  - Okić, Ivana
AU  - Ilić, Vesna
AU  - Čokić, Vladan
AU  - Santibanez, Juan F.
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/298
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Erythropoietic effects of il-17: involvement of mapks and gata transcription factors
EP  - 36
SP  - 36
VL  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rimi_298
ER  - 

@conference{
author = "Krstić, Aleksandra and Kocić, Jelena and Mojsilović, S. and Okić, Ivana and Ilić, Vesna and Čokić, Vladan and Santibanez, Juan F. and Jovčić, Gordana and Bugarski, Diana",
year = "2010",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Erythropoietic effects of il-17: involvement of mapks and gata transcription factors",
pages = "36-36",
volume = "95",
url = "https://hdl.handle.net/21.15107/rcub_rimi_298"
}

Krstić, A., Kocić, J., Mojsilović, S., Okić, I., Ilić, V., Čokić, V., Santibanez, J. F., Jovčić, G.,& Bugarski, D.. (2010). Erythropoietic effects of il-17: involvement of mapks and gata transcription factors. in Haematologica
Ferrata Storti Foundation, Pavia., 95, 36-36.
https://hdl.handle.net/21.15107/rcub_rimi_298

Krstić A, Kocić J, Mojsilović S, Okić I, Ilić V, Čokić V, Santibanez JF, Jovčić G, Bugarski D. Erythropoietic effects of il-17: involvement of mapks and gata transcription factors. in Haematologica. 2010;95:36-36.
https://hdl.handle.net/21.15107/rcub_rimi_298 .

Krstić, Aleksandra, Kocić, Jelena, Mojsilović, S., Okić, Ivana, Ilić, Vesna, Čokić, Vladan, Santibanez, Juan F., Jovčić, Gordana, Bugarski, Diana, "Erythropoietic effects of il-17: involvement of mapks and gata transcription factors" in Haematologica, 95 (2010):36-36,
https://hdl.handle.net/21.15107/rcub_rimi_298 .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Okić, Ivana
AU  - Petrićević, Tanja
AU  - Mojsilović, S.
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Milenković, Pavle B.
AU  - Petakov, Marijana
AU  - Radovanović, Anita
AU  - Božić, Tatjana
AU  - Ivanović, Z.
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/261
AB  - Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia.
AB  - Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cultivation of hamster bone marrow haematopoietic stem and progenitor cells
T1  - Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka
EP  - 14
IS  - 1
SP  - 3
VL  - 60
DO  - 10.2298/AVB1001003K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Okić, Ivana and Petrićević, Tanja and Mojsilović, S. and Krstić, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Milenković, Pavle B. and Petakov, Marijana and Radovanović, Anita and Božić, Tatjana and Ivanović, Z.",
year = "2010",
abstract = "Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia., Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells, Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka",
pages = "14-3",
number = "1",
volume = "60",
doi = "10.2298/AVB1001003K"
}

Kovačević-Filipović, M., Okić, I., Petrićević, T., Mojsilović, S., Krstić, A., Jovčić, G., Bugarski, D., Milenković, P. B., Petakov, M., Radovanović, A., Božić, T.,& Ivanović, Z.. (2010). Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1), 3-14.
https://doi.org/10.2298/AVB1001003K

Kovačević-Filipović M, Okić I, Petrićević T, Mojsilović S, Krstić A, Jovčić G, Bugarski D, Milenković PB, Petakov M, Radovanović A, Božić T, Ivanović Z. Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd. 2010;60(1):3-14.
doi:10.2298/AVB1001003K .

Kovačević-Filipović, Milica, Okić, Ivana, Petrićević, Tanja, Mojsilović, S., Krstić, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Milenković, Pavle B., Petakov, Marijana, Radovanović, Anita, Božić, Tatjana, Ivanović, Z., "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells" in Acta Veterinaria-Beograd, 60, no. 1 (2010):3-14,
https://doi.org/10.2298/AVB1001003K . .

TY  - CONF
AU  - Bugarski, Diana
AU  - Mojsilović, S.
AU  - Ilić, Vesna
AU  - Jovčić, Gordana
AU  - Krstić, Aleksandra
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/304
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Il-17 and fgf signaling involved in mesenchymal stem cell proliferation
EP  - 660
SP  - 660
VL  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rimi_304
ER  - 

@conference{
author = "Bugarski, Diana and Mojsilović, S. and Ilić, Vesna and Jovčić, Gordana and Krstić, Aleksandra",
year = "2010",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Il-17 and fgf signaling involved in mesenchymal stem cell proliferation",
pages = "660-660",
volume = "95",
url = "https://hdl.handle.net/21.15107/rcub_rimi_304"
}

Bugarski, D., Mojsilović, S., Ilić, V., Jovčić, G.,& Krstić, A.. (2010). Il-17 and fgf signaling involved in mesenchymal stem cell proliferation. in Haematologica
Ferrata Storti Foundation, Pavia., 95, 660-660.
https://hdl.handle.net/21.15107/rcub_rimi_304

Bugarski D, Mojsilović S, Ilić V, Jovčić G, Krstić A. Il-17 and fgf signaling involved in mesenchymal stem cell proliferation. in Haematologica. 2010;95:660-660.
https://hdl.handle.net/21.15107/rcub_rimi_304 .

Bugarski, Diana, Mojsilović, S., Ilić, Vesna, Jovčić, Gordana, Krstić, Aleksandra, "Il-17 and fgf signaling involved in mesenchymal stem cell proliferation" in Haematologica, 95 (2010):660-660,
https://hdl.handle.net/21.15107/rcub_rimi_304 .

TY  - CONF
AU  - Čokić, Vladan
AU  - Mojsilović, S.
AU  - Miloksević, V.
AU  - Kraguljac-Kurtović, Nada
AU  - Bogdanović, Andrija
AU  - Jovčić, Gordana
AU  - Milenković, P.
AU  - Gotić, Mirjana
AU  - Noguchi, Constance T.
AU  - Schechter, Alan N.
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/312
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia
EP  - 335
SP  - 334
VL  - 95
UR  - https://hdl.handle.net/21.15107/rcub_rimi_312
ER  - 

@conference{
author = "Čokić, Vladan and Mojsilović, S. and Miloksević, V. and Kraguljac-Kurtović, Nada and Bogdanović, Andrija and Jovčić, Gordana and Milenković, P. and Gotić, Mirjana and Noguchi, Constance T. and Schechter, Alan N.",
year = "2010",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia",
pages = "335-334",
volume = "95",
url = "https://hdl.handle.net/21.15107/rcub_rimi_312"
}

Čokić, V., Mojsilović, S., Miloksević, V., Kraguljac-Kurtović, N., Bogdanović, A., Jovčić, G., Milenković, P., Gotić, M., Noguchi, C. T.,& Schechter, A. N.. (2010). Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia. in Haematologica
Ferrata Storti Foundation, Pavia., 95, 334-335.
https://hdl.handle.net/21.15107/rcub_rimi_312

Čokić V, Mojsilović S, Miloksević V, Kraguljac-Kurtović N, Bogdanović A, Jovčić G, Milenković P, Gotić M, Noguchi CT, Schechter AN. Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia. in Haematologica. 2010;95:334-335.
https://hdl.handle.net/21.15107/rcub_rimi_312 .

Čokić, Vladan, Mojsilović, S., Miloksević, V., Kraguljac-Kurtović, Nada, Bogdanović, Andrija, Jovčić, Gordana, Milenković, P., Gotić, Mirjana, Noguchi, Constance T., Schechter, Alan N., "Gene expression profile of hematopoietic progenitor cells vs. Granulocytes in chronic myeloid leukemia" in Haematologica, 95 (2010):334-335,
https://hdl.handle.net/21.15107/rcub_rimi_312 .

TY  - CONF
AU  - Cokić, P.
AU  - Han, Jing
AU  - Mojsilović, S.
AU  - Beleslin-Čokić, Bojana
AU  - Mirković, K.
AU  - Kraguljac-Kurtović, Nada
AU  - Damjanović, Svetozar
AU  - Gotić, Mirjana
AU  - Skoda, R.
AU  - Puri, Raj K.
AU  - Noguchi, Constance T.
AU  - Schechter, Alan N.
PY  - 2009
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/251
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Gene expression profiling of cd34(+) cells in myeloproliferative disorders by cdna microarray technology
EP  - 352
SP  - 352
VL  - 94
UR  - https://hdl.handle.net/21.15107/rcub_rimi_251
ER  - 

@conference{
author = "Cokić, P. and Han, Jing and Mojsilović, S. and Beleslin-Čokić, Bojana and Mirković, K. and Kraguljac-Kurtović, Nada and Damjanović, Svetozar and Gotić, Mirjana and Skoda, R. and Puri, Raj K. and Noguchi, Constance T. and Schechter, Alan N.",
year = "2009",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Gene expression profiling of cd34(+) cells in myeloproliferative disorders by cdna microarray technology",
pages = "352-352",
volume = "94",
url = "https://hdl.handle.net/21.15107/rcub_rimi_251"
}

Cokić, P., Han, J., Mojsilović, S., Beleslin-Čokić, B., Mirković, K., Kraguljac-Kurtović, N., Damjanović, S., Gotić, M., Skoda, R., Puri, R. K., Noguchi, C. T.,& Schechter, A. N.. (2009). Gene expression profiling of cd34(+) cells in myeloproliferative disorders by cdna microarray technology. in Haematologica
Ferrata Storti Foundation, Pavia., 94, 352-352.
https://hdl.handle.net/21.15107/rcub_rimi_251

Cokić P, Han J, Mojsilović S, Beleslin-Čokić B, Mirković K, Kraguljac-Kurtović N, Damjanović S, Gotić M, Skoda R, Puri RK, Noguchi CT, Schechter AN. Gene expression profiling of cd34(+) cells in myeloproliferative disorders by cdna microarray technology. in Haematologica. 2009;94:352-352.
https://hdl.handle.net/21.15107/rcub_rimi_251 .

Cokić, P., Han, Jing, Mojsilović, S., Beleslin-Čokić, Bojana, Mirković, K., Kraguljac-Kurtović, Nada, Damjanović, Svetozar, Gotić, Mirjana, Skoda, R., Puri, Raj K., Noguchi, Constance T., Schechter, Alan N., "Gene expression profiling of cd34(+) cells in myeloproliferative disorders by cdna microarray technology" in Haematologica, 94 (2009):352-352,
https://hdl.handle.net/21.15107/rcub_rimi_251 .

TY  - JOUR
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Krstić, A.
AU  - Vlaški, Marija
AU  - Petakov, Marijana
AU  - Mojsilović, S.
AU  - Stojanović, N.
AU  - Milenković, P.
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/160
AB  - To evaluate whether the response of hematopoietic cells to interleukin-17 (IL-17) depends on the tissue microenvironment in which hematopoiesis occurs, the influence of recombinant mouse IL-17 on spleen hematopoietic cells and cytokine release was assessed in normal mice in vitro and in vivo. In vitro, IL-17 did not significantly affect the growth of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E and CFU-E) derived colonies. A single injection of IL-17 in vivo exhibited stimulatory effects on hematopoietic cells from both granulocytic and erythroid lineages. The increased number of metamyelocytes 48 h after treatment imply to the IL-17-induced stimulation of granulopoiesis. The number of BFU-E was increased at 24 h, while the number of CFU-E increased 6 h and 24 h after treatment. Since the same treatment in the bone marrow decreased the number of CFU-E, it may be concluded that the local microenvironment plays an important role in IL-17-mediated effects on CFU-E. IL-17 increased the release of IL-6 both in vitro and in vivo, but showed tendency to suppress the constitutive secretion of IL-10 by spleen cells. Our results suggest the complexity of target cell response and interplay of secondary induced cytokines by IL-17 in different hematopoietic organs.
PB  - Acad Sciences Czech Republic, Inst Physiology, Prague 4
T2  - Physiological Research
T1  - The effect of interieukin-17 on hematopoietic cells and cytokine release in mouse spleen
EP  - 339
IS  - 3
SP  - 331
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_rimi_160
ER  - 

@article{
author = "Jovčić, Gordana and Bugarski, Diana and Krstić, A. and Vlaški, Marija and Petakov, Marijana and Mojsilović, S. and Stojanović, N. and Milenković, P.",
year = "2007",
abstract = "To evaluate whether the response of hematopoietic cells to interleukin-17 (IL-17) depends on the tissue microenvironment in which hematopoiesis occurs, the influence of recombinant mouse IL-17 on spleen hematopoietic cells and cytokine release was assessed in normal mice in vitro and in vivo. In vitro, IL-17 did not significantly affect the growth of granulocyte-macrophage (CFU-GM) and erythroid (BFU-E and CFU-E) derived colonies. A single injection of IL-17 in vivo exhibited stimulatory effects on hematopoietic cells from both granulocytic and erythroid lineages. The increased number of metamyelocytes 48 h after treatment imply to the IL-17-induced stimulation of granulopoiesis. The number of BFU-E was increased at 24 h, while the number of CFU-E increased 6 h and 24 h after treatment. Since the same treatment in the bone marrow decreased the number of CFU-E, it may be concluded that the local microenvironment plays an important role in IL-17-mediated effects on CFU-E. IL-17 increased the release of IL-6 both in vitro and in vivo, but showed tendency to suppress the constitutive secretion of IL-10 by spleen cells. Our results suggest the complexity of target cell response and interplay of secondary induced cytokines by IL-17 in different hematopoietic organs.",
publisher = "Acad Sciences Czech Republic, Inst Physiology, Prague 4",
journal = "Physiological Research",
title = "The effect of interieukin-17 on hematopoietic cells and cytokine release in mouse spleen",
pages = "339-331",
number = "3",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_rimi_160"
}

Jovčić, G., Bugarski, D., Krstić, A., Vlaški, M., Petakov, M., Mojsilović, S., Stojanović, N.,& Milenković, P.. (2007). The effect of interieukin-17 on hematopoietic cells and cytokine release in mouse spleen. in Physiological Research
Acad Sciences Czech Republic, Inst Physiology, Prague 4., 56(3), 331-339.
https://hdl.handle.net/21.15107/rcub_rimi_160

Jovčić G, Bugarski D, Krstić A, Vlaški M, Petakov M, Mojsilović S, Stojanović N, Milenković P. The effect of interieukin-17 on hematopoietic cells and cytokine release in mouse spleen. in Physiological Research. 2007;56(3):331-339.
https://hdl.handle.net/21.15107/rcub_rimi_160 .

Jovčić, Gordana, Bugarski, Diana, Krstić, A., Vlaški, Marija, Petakov, Marijana, Mojsilović, S., Stojanović, N., Milenković, P., "The effect of interieukin-17 on hematopoietic cells and cytokine release in mouse spleen" in Physiological Research, 56, no. 3 (2007):331-339,
https://hdl.handle.net/21.15107/rcub_rimi_160 .

TY  - CONF
AU  - Dokmanovic-Miletić, M. P.
AU  - Stojanović, R.M.
AU  - Pajić, O.
AU  - Bugarski, Diana
AU  - Mojsilović, S.
AU  - Milenković, Pavle B.
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/183
PB  - BMJ Publishing Group, London
C3  - Annals of the Rheumatic Diseases
T1  - Concentrations of IL-17 and autoantibodies in sera of patients with primary Sjogren's syndrome
EP  - 140
SP  - 140
VL  - 66
UR  - https://hdl.handle.net/21.15107/rcub_rimi_183
ER  - 

@conference{
author = "Dokmanovic-Miletić, M. P. and Stojanović, R.M. and Pajić, O. and Bugarski, Diana and Mojsilović, S. and Milenković, Pavle B.",
year = "2007",
publisher = "BMJ Publishing Group, London",
journal = "Annals of the Rheumatic Diseases",
title = "Concentrations of IL-17 and autoantibodies in sera of patients with primary Sjogren's syndrome",
pages = "140-140",
volume = "66",
url = "https://hdl.handle.net/21.15107/rcub_rimi_183"
}

Dokmanovic-Miletić, M. P., Stojanović, R.M., Pajić, O., Bugarski, D., Mojsilović, S.,& Milenković, P. B.. (2007). Concentrations of IL-17 and autoantibodies in sera of patients with primary Sjogren's syndrome. in Annals of the Rheumatic Diseases
BMJ Publishing Group, London., 66, 140-140.
https://hdl.handle.net/21.15107/rcub_rimi_183

Dokmanovic-Miletić MP, Stojanović R, Pajić O, Bugarski D, Mojsilović S, Milenković PB. Concentrations of IL-17 and autoantibodies in sera of patients with primary Sjogren's syndrome. in Annals of the Rheumatic Diseases. 2007;66:140-140.
https://hdl.handle.net/21.15107/rcub_rimi_183 .

Dokmanovic-Miletić, M. P., Stojanović, R.M., Pajić, O., Bugarski, Diana, Mojsilović, S., Milenković, Pavle B., "Concentrations of IL-17 and autoantibodies in sera of patients with primary Sjogren's syndrome" in Annals of the Rheumatic Diseases, 66 (2007):140-140,
https://hdl.handle.net/21.15107/rcub_rimi_183 .

TY  - JOUR
AU  - Lukić, A.
AU  - Vasilijić, S.
AU  - Majstorović, Ivana
AU  - Vucević, D.
AU  - Mojsilović, S.
AU  - Gazivoda, D.
AU  - Danilović, V.
AU  - Petrović, R.
AU  - Colić, M.
PY  - 2006
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/149
AB  - Aim To analyse phenotypic characteristics of antigen-presenting cells (APC), isolated from human periapical lesions by flow cytometry and immunocytochemistry. Methodology Sixteen periapical lesions were digested for 15 min with 0.05% collagenase. Mononuclear cells, separated from other inflammatory cells by density centrifugation, were processed for flow cytometry and/or immunocytochemistry. Single and double immunostainings were performed using monoclonal antibodies specific for human CD45, CD3, CD19, CD14, HLA-DR, CD1a, CD83 and CD123. Results Antigen-presenting cells (HLA-DR+ cells) represented 32.9 +/- 17.8% of total mononuclear cells. Amongst them, B cells (HLA-DR+ CD19(+)) were the predominant APC population, followed by activated macrophages (HLA-DR+ CD14(+)), dendritic cells (DC) (HLA-DR+ CD14(-) CD19(-) CD3(-)) and activated T cells (HLA-DR+ CD3(+)). Based on the predominance of T cells (CD3(+)) or B cells and plasma cells (CD19(+) and CD19(lo), respectively) amongst mononuclear cell infiltrates, lesions were divided into T- and B-types. The percentage of DC in T-type lesions (27.1 +/- 6.8% of total HLA-DR+ cells) was higher, compared with B-type lesions (10.3 +/- 5.2%) (P  lt  0.01). Within the DC population, the percentages of CD1a (Langerhans cell type) and CD123 (probably plasmacytoid DC type) did not differ significantly between the groups (P  gt  0.05). However, the percentage of mature DC (CD83(+)) was significantly higher in T-type periapical lesions (P  lt  0.05). Conclusion Flow cytometry and immunocytochemistry are suitable methods for phenotypic analysis of APC after their isolation from human periapical lesions. APC, that were phenotypically heterogeneous, constituted a significant component of infiltrating cells. Lesions with the predominance of T cells were characterized by a higher proportion of mature DC (HLA-DR(+)CD83(+) cells) than lesions with predominance of B cells/plasma cells.
PB  - Wiley, Hoboken
T2  - International Endodontic Journal
T1  - Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry
EP  - 636
IS  - 8
SP  - 626
VL  - 39
DO  - 10.1111/j.1365-2591.2006.01125.x
ER  - 

@article{
author = "Lukić, A. and Vasilijić, S. and Majstorović, Ivana and Vucević, D. and Mojsilović, S. and Gazivoda, D. and Danilović, V. and Petrović, R. and Colić, M.",
year = "2006",
abstract = "Aim To analyse phenotypic characteristics of antigen-presenting cells (APC), isolated from human periapical lesions by flow cytometry and immunocytochemistry. Methodology Sixteen periapical lesions were digested for 15 min with 0.05% collagenase. Mononuclear cells, separated from other inflammatory cells by density centrifugation, were processed for flow cytometry and/or immunocytochemistry. Single and double immunostainings were performed using monoclonal antibodies specific for human CD45, CD3, CD19, CD14, HLA-DR, CD1a, CD83 and CD123. Results Antigen-presenting cells (HLA-DR+ cells) represented 32.9 +/- 17.8% of total mononuclear cells. Amongst them, B cells (HLA-DR+ CD19(+)) were the predominant APC population, followed by activated macrophages (HLA-DR+ CD14(+)), dendritic cells (DC) (HLA-DR+ CD14(-) CD19(-) CD3(-)) and activated T cells (HLA-DR+ CD3(+)). Based on the predominance of T cells (CD3(+)) or B cells and plasma cells (CD19(+) and CD19(lo), respectively) amongst mononuclear cell infiltrates, lesions were divided into T- and B-types. The percentage of DC in T-type lesions (27.1 +/- 6.8% of total HLA-DR+ cells) was higher, compared with B-type lesions (10.3 +/- 5.2%) (P  lt  0.01). Within the DC population, the percentages of CD1a (Langerhans cell type) and CD123 (probably plasmacytoid DC type) did not differ significantly between the groups (P  gt  0.05). However, the percentage of mature DC (CD83(+)) was significantly higher in T-type periapical lesions (P  lt  0.05). Conclusion Flow cytometry and immunocytochemistry are suitable methods for phenotypic analysis of APC after their isolation from human periapical lesions. APC, that were phenotypically heterogeneous, constituted a significant component of infiltrating cells. Lesions with the predominance of T cells were characterized by a higher proportion of mature DC (HLA-DR(+)CD83(+) cells) than lesions with predominance of B cells/plasma cells.",
publisher = "Wiley, Hoboken",
journal = "International Endodontic Journal",
title = "Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry",
pages = "636-626",
number = "8",
volume = "39",
doi = "10.1111/j.1365-2591.2006.01125.x"
}

Lukić, A., Vasilijić, S., Majstorović, I., Vucević, D., Mojsilović, S., Gazivoda, D., Danilović, V., Petrović, R.,& Colić, M.. (2006). Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry. in International Endodontic Journal
Wiley, Hoboken., 39(8), 626-636.
https://doi.org/10.1111/j.1365-2591.2006.01125.x

Lukić A, Vasilijić S, Majstorović I, Vucević D, Mojsilović S, Gazivoda D, Danilović V, Petrović R, Colić M. Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry. in International Endodontic Journal. 2006;39(8):626-636.
doi:10.1111/j.1365-2591.2006.01125.x .

Lukić, A., Vasilijić, S., Majstorović, Ivana, Vucević, D., Mojsilović, S., Gazivoda, D., Danilović, V., Petrović, R., Colić, M., "Characterization of antigen-presenting cells in human apical periodontitis lesions by flow cytometry and immunocytochemistry" in International Endodontic Journal, 39, no. 8 (2006):626-636,
https://doi.org/10.1111/j.1365-2591.2006.01125.x . .

TY  - JOUR
AU  - Colić, M.
AU  - Mojsilović, S.
AU  - Pavlović, B.
AU  - Vucićević, D.
AU  - Majstorović, Ivana
AU  - Bufan, Bijana
AU  - Stojić-Vukanić, Z.
AU  - Vasilijić, S.
AU  - Vucević, D.
AU  - Gasić, S.
AU  - Balint, Bela
PY  - 2004
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/107
AB  - BACKGROUND: Dendritic cells (DC) have been used for immunotherapy of malignant tumors, different kinds of infections, and other clinical conditions. For that purpose, optimal conditions for the generation of functionally mature DC in vitro are required. Two different protocols for the induction of maturation of monocyte-derived DC (MDDC) were compared in this study. METHODS: MDDC were generated in vitro by cultivating adherent monocytes of healthy volunteers with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) during 6-days period. The immature DC thus prepared were induced to mature using two protocols. DC were stimulated for 2 days with lipopolysaccharide (LPS), or with a cocktail of proinflammatory mediators (PM) containing IL-1beta, IL-6, tumor necrosis factor alpha (TNFalpha), and prostaglandin E2 (PGE2), respectively. Phenotypic characteristics of MDDC and their endocytic activity were studied by flow cytometry. Allostimulatory activity of these cells was tested in the mixed leukocyte reaction (MLR), whereas the production of cytokines was determined by ELISA kits. RESULTS: MDDC matured with PM (PM-DC) were predominantly non-adherent cells, while about 30% of LPS-matured DC were adherent cells. In comparison with LPS-DC, PM-DC expressed higher levels of CD86 and CD83, had lower endocytic activity, produced higher levels of IL-10 and lower levels of IL-12, and more strongly stimulated proliferation of allogeneic lymphocytes. CONCLUSION: The protocol based on the combination of proinflammatory cytokines and PGE2 is better for the induction of maturation of human MDDC in vitro than the protocol using LPS alone.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro.
EP  - 478
IS  - 5
SP  - 471
VL  - 61
DO  - 10.2298/VSP0405471C
ER  - 

@article{
author = "Colić, M. and Mojsilović, S. and Pavlović, B. and Vucićević, D. and Majstorović, Ivana and Bufan, Bijana and Stojić-Vukanić, Z. and Vasilijić, S. and Vucević, D. and Gasić, S. and Balint, Bela",
year = "2004",
abstract = "BACKGROUND: Dendritic cells (DC) have been used for immunotherapy of malignant tumors, different kinds of infections, and other clinical conditions. For that purpose, optimal conditions for the generation of functionally mature DC in vitro are required. Two different protocols for the induction of maturation of monocyte-derived DC (MDDC) were compared in this study. METHODS: MDDC were generated in vitro by cultivating adherent monocytes of healthy volunteers with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) during 6-days period. The immature DC thus prepared were induced to mature using two protocols. DC were stimulated for 2 days with lipopolysaccharide (LPS), or with a cocktail of proinflammatory mediators (PM) containing IL-1beta, IL-6, tumor necrosis factor alpha (TNFalpha), and prostaglandin E2 (PGE2), respectively. Phenotypic characteristics of MDDC and their endocytic activity were studied by flow cytometry. Allostimulatory activity of these cells was tested in the mixed leukocyte reaction (MLR), whereas the production of cytokines was determined by ELISA kits. RESULTS: MDDC matured with PM (PM-DC) were predominantly non-adherent cells, while about 30% of LPS-matured DC were adherent cells. In comparison with LPS-DC, PM-DC expressed higher levels of CD86 and CD83, had lower endocytic activity, produced higher levels of IL-10 and lower levels of IL-12, and more strongly stimulated proliferation of allogeneic lymphocytes. CONCLUSION: The protocol based on the combination of proinflammatory cytokines and PGE2 is better for the induction of maturation of human MDDC in vitro than the protocol using LPS alone.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro.",
pages = "478-471",
number = "5",
volume = "61",
doi = "10.2298/VSP0405471C"
}

Colić, M., Mojsilović, S., Pavlović, B., Vucićević, D., Majstorović, I., Bufan, B., Stojić-Vukanić, Z., Vasilijić, S., Vucević, D., Gasić, S.,& Balint, B.. (2004). Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro.. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 61(5), 471-478.
https://doi.org/10.2298/VSP0405471C

Colić M, Mojsilović S, Pavlović B, Vucićević D, Majstorović I, Bufan B, Stojić-Vukanić Z, Vasilijić S, Vucević D, Gasić S, Balint B. Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro.. in Vojnosanitetski pregled. 2004;61(5):471-478.
doi:10.2298/VSP0405471C .

Colić, M., Mojsilović, S., Pavlović, B., Vucićević, D., Majstorović, Ivana, Bufan, Bijana, Stojić-Vukanić, Z., Vasilijić, S., Vucević, D., Gasić, S., Balint, Bela, "Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro." in Vojnosanitetski pregled, 61, no. 5 (2004):471-478,
https://doi.org/10.2298/VSP0405471C . .