TY  - JOUR
AU  - Vignjević, Sanja
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Mojsilović, Slavko
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Beleslin-Čokić, Bojana
AU  - Čokić, Vladan
AU  - Jovčić, Gordana
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/575
AB  - Psychological stress affects different physiological processes including haematopoiesis. However, erythropoietic effects of chronic psychological stress remain largely unknown. The adult spleen contains a distinct microenvironment favourable for rapid expansion of erythroid progenitors in response to stressful stimuli, and emerging evidence suggests that inappropriate activation of stress erythropoiesis may predispose to leukaemic transformation. We used a mouse model to study the influence of chronic psychological stress on erythropoiesis in the spleen and to investigate potential mediators of observed effects. Adult mice were subjected to 2hrs daily restraint stress for 7 or 14 consecutive days. Our results showed that chronic exposure to restraint stress decreased the concentration of haemoglobin in the blood, elevated circulating levels of erythropoietin and corticosterone, and resulted in markedly increased number of erythroid progenitors and precursors in the spleen. Western blot analysis revealed significantly decreased expression of both erythropoietin receptor and glucocorticoid receptor in the spleen of restrained mice. Furthermore, chronic stress enhanced the expression of stem cell factor receptor in the red pulp. Moreover, chronically stressed animals exhibited significantly increased expression of bone morphogenetic protein 4 (BMP4) in the red pulp as well as substantially enhanced mRNA expression levels of its receptors in the spleen. These findings demonstrate for the first time that chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis and leads to the prolonged activation of stress erythropoiesis pathways. Prolonged activation of these pathways along with an excessive production of immature erythroid cells may predispose chronically stressed subjects to a higher risk of leukaemic transformation.
PB  - Wiley, Hoboken
T2  - Journal of Cellular & Molecular Medicine
T1  - Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis
EP  - 103
IS  - 1
SP  - 91
VL  - 18
DO  - 10.1111/jcmm.12167
ER  - 

@article{
author = "Vignjević, Sanja and Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Mojsilović, Slavko and Vranješ-Đurić, Sanja and Koko, Vesna and Beleslin-Čokić, Bojana and Čokić, Vladan and Jovčić, Gordana",
year = "2014",
abstract = "Psychological stress affects different physiological processes including haematopoiesis. However, erythropoietic effects of chronic psychological stress remain largely unknown. The adult spleen contains a distinct microenvironment favourable for rapid expansion of erythroid progenitors in response to stressful stimuli, and emerging evidence suggests that inappropriate activation of stress erythropoiesis may predispose to leukaemic transformation. We used a mouse model to study the influence of chronic psychological stress on erythropoiesis in the spleen and to investigate potential mediators of observed effects. Adult mice were subjected to 2hrs daily restraint stress for 7 or 14 consecutive days. Our results showed that chronic exposure to restraint stress decreased the concentration of haemoglobin in the blood, elevated circulating levels of erythropoietin and corticosterone, and resulted in markedly increased number of erythroid progenitors and precursors in the spleen. Western blot analysis revealed significantly decreased expression of both erythropoietin receptor and glucocorticoid receptor in the spleen of restrained mice. Furthermore, chronic stress enhanced the expression of stem cell factor receptor in the red pulp. Moreover, chronically stressed animals exhibited significantly increased expression of bone morphogenetic protein 4 (BMP4) in the red pulp as well as substantially enhanced mRNA expression levels of its receptors in the spleen. These findings demonstrate for the first time that chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis and leads to the prolonged activation of stress erythropoiesis pathways. Prolonged activation of these pathways along with an excessive production of immature erythroid cells may predispose chronically stressed subjects to a higher risk of leukaemic transformation.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular & Molecular Medicine",
title = "Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis",
pages = "103-91",
number = "1",
volume = "18",
doi = "10.1111/jcmm.12167"
}

Vignjević, S., Budeč, M., Marković, D., Đikić, D., Mitrović, O., Mojsilović, S., Vranješ-Đurić, S., Koko, V., Beleslin-Čokić, B., Čokić, V.,& Jovčić, G.. (2014). Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. in Journal of Cellular & Molecular Medicine
Wiley, Hoboken., 18(1), 91-103.
https://doi.org/10.1111/jcmm.12167

Vignjević S, Budeč M, Marković D, Đikić D, Mitrović O, Mojsilović S, Vranješ-Đurić S, Koko V, Beleslin-Čokić B, Čokić V, Jovčić G. Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis. in Journal of Cellular & Molecular Medicine. 2014;18(1):91-103.
doi:10.1111/jcmm.12167 .

Vignjević, Sanja, Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Mitrović, Olivera, Mojsilović, Slavko, Vranješ-Đurić, Sanja, Koko, Vesna, Beleslin-Čokić, Bojana, Čokić, Vladan, Jovčić, Gordana, "Chronic psychological stress activates BMP4-dependent extramedullary erythropoiesis" in Journal of Cellular & Molecular Medicine, 18, no. 1 (2014):91-103,
https://doi.org/10.1111/jcmm.12167 . .

TY  - JOUR
AU  - Petrović-Kosanović, Dragana
AU  - Ukropina, Mirela
AU  - Čakić-Milošević, Maja
AU  - Budeč, Mirela
AU  - Milošević, Verica
AU  - Koko, Vesna
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/519
AB  - Immunohistochemistry revealed the presence of Vasoactive Intestinal Peptide (VIP), Neuropeptide Y (NPY), and the absence of Substance P (SP) immunoreactivity in the rat adrenal cortex. VIP- and NPY-immunoreactivity were detected in nerve fibers around the small blood vessels projecting into the capsule and cortical zones surrounding blood vessels and cortical cells. After acute heat stress, VIP- and NPY-immunoreactivities in the nerve fibers were reduced, probably as a result of the release of these peptides.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress
EP  - 320
IS  - 1
SP  - 315
VL  - 65
DO  - 10.2298/ABS1301315K
ER  - 

@article{
author = "Petrović-Kosanović, Dragana and Ukropina, Mirela and Čakić-Milošević, Maja and Budeč, Mirela and Milošević, Verica and Koko, Vesna",
year = "2013",
abstract = "Immunohistochemistry revealed the presence of Vasoactive Intestinal Peptide (VIP), Neuropeptide Y (NPY), and the absence of Substance P (SP) immunoreactivity in the rat adrenal cortex. VIP- and NPY-immunoreactivity were detected in nerve fibers around the small blood vessels projecting into the capsule and cortical zones surrounding blood vessels and cortical cells. After acute heat stress, VIP- and NPY-immunoreactivities in the nerve fibers were reduced, probably as a result of the release of these peptides.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress",
pages = "320-315",
number = "1",
volume = "65",
doi = "10.2298/ABS1301315K"
}

Petrović-Kosanović, D., Ukropina, M., Čakić-Milošević, M., Budeč, M., Milošević, V.,& Koko, V.. (2013). Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(1), 315-320.
https://doi.org/10.2298/ABS1301315K

Petrović-Kosanović D, Ukropina M, Čakić-Milošević M, Budeč M, Milošević V, Koko V. Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress. in Archives of Biological Sciences. 2013;65(1):315-320.
doi:10.2298/ABS1301315K .

Petrović-Kosanović, Dragana, Ukropina, Mirela, Čakić-Milošević, Maja, Budeč, Mirela, Milošević, Verica, Koko, Vesna, "Immunohistochemical evidence for the presence of a Vasoactive Intestinal Peptide, Neuropeptide Y, and Substance P, in rat adrenal cortex after acute heat stress" in Archives of Biological Sciences, 65, no. 1 (2013):315-320,
https://doi.org/10.2298/ABS1301315K . .

TY  - JOUR
AU  - Mitrović, Olivera
AU  - Mićić, Mileva
AU  - Čokić, Vladan
AU  - Koko, Vesna
AU  - Đikić, Dragoslava
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Breković, Tijana
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/465
AB  - We studied TOP2A amplification (using FISH methods), and TOP2A and BRCA1 protein overexpression (immunohistochemistry) in four molecular subtypes of breast cancer. Of 53 patients, 32 showed TOP2A and 38 showed BRCA1 overexpression. The highest percentage of TOP2A amplification (47.8%) and deletion (13%) was detected in Luminal B subtypes. Of 11 Luminal B tumors with TOP2A amplification, 9 (81.8%) overexpressed TOP2A. BRCA1 protein overexpression showed significant positive correlation with TOP2A protein expression. BRCA1 and TOP2A proteins exhibited similar patterns of expression in Luminal B and triple-negative breast cancer, suggesting the same prognosis in those patients.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer
EP  - 518
IS  - 2
SP  - 511
VL  - 65
DO  - 10.2298/ABS1302511M
ER  - 

@article{
author = "Mitrović, Olivera and Mićić, Mileva and Čokić, Vladan and Koko, Vesna and Đikić, Dragoslava and Budeč, Mirela and Vignjević, Sanja and Breković, Tijana",
year = "2013",
abstract = "We studied TOP2A amplification (using FISH methods), and TOP2A and BRCA1 protein overexpression (immunohistochemistry) in four molecular subtypes of breast cancer. Of 53 patients, 32 showed TOP2A and 38 showed BRCA1 overexpression. The highest percentage of TOP2A amplification (47.8%) and deletion (13%) was detected in Luminal B subtypes. Of 11 Luminal B tumors with TOP2A amplification, 9 (81.8%) overexpressed TOP2A. BRCA1 protein overexpression showed significant positive correlation with TOP2A protein expression. BRCA1 and TOP2A proteins exhibited similar patterns of expression in Luminal B and triple-negative breast cancer, suggesting the same prognosis in those patients.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer",
pages = "518-511",
number = "2",
volume = "65",
doi = "10.2298/ABS1302511M"
}

Mitrović, O., Mićić, M., Čokić, V., Koko, V., Đikić, D., Budeč, M., Vignjević, S.,& Breković, T.. (2013). BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(2), 511-518.
https://doi.org/10.2298/ABS1302511M

Mitrović O, Mićić M, Čokić V, Koko V, Đikić D, Budeč M, Vignjević S, Breković T. BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer. in Archives of Biological Sciences. 2013;65(2):511-518.
doi:10.2298/ABS1302511M .

Mitrović, Olivera, Mićić, Mileva, Čokić, Vladan, Koko, Vesna, Đikić, Dragoslava, Budeč, Mirela, Vignjević, Sanja, Breković, Tijana, "BRCA1 and TOP2A gene amplification and protein expression in four molecular subtypes of breast cancer" in Archives of Biological Sciences, 65, no. 2 (2013):511-518,
https://doi.org/10.2298/ABS1302511M . .

TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Budeč, Mirela
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Vignjević, Sanja
AU  - Mitrović, Olivera
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/505
AB  - Objective. Although it is known that nitric oxide modulates the activity of hypothalamic-pituitary-adrenal axis, its functional significance has not been elucidated. Additionally, there is no information on the effect of nitric oxide on cortical expression of glucocorticoid receptor. The purpose of this study was to investigate the influence of endogenous nitric oxide on structure and function of rat adrenal cortex and adrenocortical expression of glucocorticoid receptor. Methods. Adult female Wistar rats showing diestrus day 1 were treated with Nω-nitro-L-arginine-methyl ester (LNAME), an inhibitor of all three isoforms of nitric oxide synthase, at a dose of 30 mg/kg, subcutaneously. The concentrations of adrenocorticotropic hormone (ACTH) and corticosterone were determined by radioimmunoassay. Stereological and immunohistochemical analyses were performed on paraffin sections. Results. Blockade of nitric oxide production significantly increased blood levels of ACTH and corticosterone. Stereological analysis showed that treatment with L-NAME significantly decreased numerical density of the cells in all cortical zones. Consistent with the decreased numerical density, L-NAME significantly increased the volume of cells in cortical zones. Inhibition of nitric oxide synthesis decreased expression of glucocorticoid receptor in zona fasciculata and zona reticularis. Conclusion. Obtained results indicate that endogenous nitric oxide inhibits activity of adrenal cortex and modulates expression of glucocorticoid receptor.
AB  - Cilj. Iako je poznato da azot-monoksid moduliše aktivnost hipotalamo-hipofizno-nadbubrežne osovine, funkcionalni značaj tog delovanja nije rasvetljen. Pored toga, dejstvo azot-monoksida na kortikalnu ekspresiju glukokortikoidnog receptora nije istraženo. Cilj ovog rada bio je da se ispita uticaj endogenog azot-monoksida na strukturu i funkciju kore nadbubrežne žlezde pacova i adrenokortikalnu ekspresiju glukokortikoidnog receptora. Metode. Odrasle ženke pacova Wistar soja u metestrusnoj fazi ciklusa tretirane su Nω-nitro-L-arginin metil esterom (L-NAME), inhibitorom sve tri izoforme azotmonoksid sintaze, u dozi 30 mg/kg, subkutano. Koncentracije adrenokortikotropnog hormona (ACTH) i kortikosterona u krvi određene su radioimunološkom metodom. Na parafinskim presecima nadbubrežne žlezde izvršena je stereološka i imunohistohemijska analiza korteksa. Rezultati. Blokada sinteze azot-monoksida značajno povećava koncentracije ACTH i kortikosterona u krvi. Stereološka analiza je pokazala da tretman sa L-NAME značajno smanjuje numeričku gustinu ćelija u svim kortikalnim zonama. U skladu sa smanjenom numeričkom gustinom, L-NAME značajno povećava volumen ćelija u sve tri zone korteksa. Inhibicija sinteze azot-monoksida smanjuje ekspresiju glukokortikoidnog receptora u zoni fascikulati i zoni retikularis. Zaključak. Dobijeni rezultati ukazuju na mogućnost da endogeni azot-monoksid inhibiše aktivnost kore nadbubrežne žlezde i da moduliše ekspresiju glukokortikoidnog receptora.
PB  - Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac
T2  - Medicinski časopis
T1  - Blockade of nitric oxide synthesis stimulates the activity of adrenal cortex
T1  - Blokada sinteze azot-monoksida stimuliše aktivnost korteksa nadbubrežne žlezde
EP  - 11
IS  - 1
SP  - 7
VL  - 47
DO  - 10.5937/mckg47-3046
ER  - 

@article{
author = "Đikić, Dragoslava and Budeč, Mirela and Vranješ-Đurić, Sanja and Koko, Vesna and Vignjević, Sanja and Mitrović, Olivera",
year = "2013",
abstract = "Objective. Although it is known that nitric oxide modulates the activity of hypothalamic-pituitary-adrenal axis, its functional significance has not been elucidated. Additionally, there is no information on the effect of nitric oxide on cortical expression of glucocorticoid receptor. The purpose of this study was to investigate the influence of endogenous nitric oxide on structure and function of rat adrenal cortex and adrenocortical expression of glucocorticoid receptor. Methods. Adult female Wistar rats showing diestrus day 1 were treated with Nω-nitro-L-arginine-methyl ester (LNAME), an inhibitor of all three isoforms of nitric oxide synthase, at a dose of 30 mg/kg, subcutaneously. The concentrations of adrenocorticotropic hormone (ACTH) and corticosterone were determined by radioimmunoassay. Stereological and immunohistochemical analyses were performed on paraffin sections. Results. Blockade of nitric oxide production significantly increased blood levels of ACTH and corticosterone. Stereological analysis showed that treatment with L-NAME significantly decreased numerical density of the cells in all cortical zones. Consistent with the decreased numerical density, L-NAME significantly increased the volume of cells in cortical zones. Inhibition of nitric oxide synthesis decreased expression of glucocorticoid receptor in zona fasciculata and zona reticularis. Conclusion. Obtained results indicate that endogenous nitric oxide inhibits activity of adrenal cortex and modulates expression of glucocorticoid receptor., Cilj. Iako je poznato da azot-monoksid moduliše aktivnost hipotalamo-hipofizno-nadbubrežne osovine, funkcionalni značaj tog delovanja nije rasvetljen. Pored toga, dejstvo azot-monoksida na kortikalnu ekspresiju glukokortikoidnog receptora nije istraženo. Cilj ovog rada bio je da se ispita uticaj endogenog azot-monoksida na strukturu i funkciju kore nadbubrežne žlezde pacova i adrenokortikalnu ekspresiju glukokortikoidnog receptora. Metode. Odrasle ženke pacova Wistar soja u metestrusnoj fazi ciklusa tretirane su Nω-nitro-L-arginin metil esterom (L-NAME), inhibitorom sve tri izoforme azotmonoksid sintaze, u dozi 30 mg/kg, subkutano. Koncentracije adrenokortikotropnog hormona (ACTH) i kortikosterona u krvi određene su radioimunološkom metodom. Na parafinskim presecima nadbubrežne žlezde izvršena je stereološka i imunohistohemijska analiza korteksa. Rezultati. Blokada sinteze azot-monoksida značajno povećava koncentracije ACTH i kortikosterona u krvi. Stereološka analiza je pokazala da tretman sa L-NAME značajno smanjuje numeričku gustinu ćelija u svim kortikalnim zonama. U skladu sa smanjenom numeričkom gustinom, L-NAME značajno povećava volumen ćelija u sve tri zone korteksa. Inhibicija sinteze azot-monoksida smanjuje ekspresiju glukokortikoidnog receptora u zoni fascikulati i zoni retikularis. Zaključak. Dobijeni rezultati ukazuju na mogućnost da endogeni azot-monoksid inhibiše aktivnost kore nadbubrežne žlezde i da moduliše ekspresiju glukokortikoidnog receptora.",
publisher = "Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac",
journal = "Medicinski časopis",
title = "Blockade of nitric oxide synthesis stimulates the activity of adrenal cortex, Blokada sinteze azot-monoksida stimuliše aktivnost korteksa nadbubrežne žlezde",
pages = "11-7",
number = "1",
volume = "47",
doi = "10.5937/mckg47-3046"
}

Đikić, D., Budeč, M., Vranješ-Đurić, S., Koko, V., Vignjević, S.,& Mitrović, O.. (2013). Blockade of nitric oxide synthesis stimulates the activity of adrenal cortex. in Medicinski časopis
Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac., 47(1), 7-11.
https://doi.org/10.5937/mckg47-3046

Đikić D, Budeč M, Vranješ-Đurić S, Koko V, Vignjević S, Mitrović O. Blockade of nitric oxide synthesis stimulates the activity of adrenal cortex. in Medicinski časopis. 2013;47(1):7-11.
doi:10.5937/mckg47-3046 .

Đikić, Dragoslava, Budeč, Mirela, Vranješ-Đurić, Sanja, Koko, Vesna, Vignjević, Sanja, Mitrović, Olivera, "Blockade of nitric oxide synthesis stimulates the activity of adrenal cortex" in Medicinski časopis, 47, no. 1 (2013):7-11,
https://doi.org/10.5937/mckg47-3046 . .

TY  - CONF
AU  - Budeč, Mirela
AU  - Vignjević, Sanja
AU  - Marković, Dragana
AU  - Jovčić, Gordana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Čokić, Vladan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/520
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica
T1  - Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling
EP  - 418
SP  - 418
VL  - 98
UR  - https://hdl.handle.net/21.15107/rcub_rimi_520
ER  - 

@conference{
author = "Budeč, Mirela and Vignjević, Sanja and Marković, Dragana and Jovčić, Gordana and Đikić, Dragoslava and Mitrović, Olivera and Vranješ-Đurić, Sanja and Koko, Vesna and Čokić, Vladan",
year = "2013",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica",
title = "Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling",
pages = "418-418",
volume = "98",
url = "https://hdl.handle.net/21.15107/rcub_rimi_520"
}

Budeč, M., Vignjević, S., Marković, D., Jovčić, G., Đikić, D., Mitrović, O., Vranješ-Đurić, S., Koko, V.,& Čokić, V.. (2013). Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling. in Haematologica
Ferrata Storti Foundation, Pavia., 98, 418-418.
https://hdl.handle.net/21.15107/rcub_rimi_520

Budeč M, Vignjević S, Marković D, Jovčić G, Đikić D, Mitrović O, Vranješ-Đurić S, Koko V, Čokić V. Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling. in Haematologica. 2013;98:418-418.
https://hdl.handle.net/21.15107/rcub_rimi_520 .

Budeč, Mirela, Vignjević, Sanja, Marković, Dragana, Jovčić, Gordana, Đikić, Dragoslava, Mitrović, Olivera, Vranješ-Đurić, Sanja, Koko, Vesna, Čokić, Vladan, "Effects of chronic psychological stress on extra-medullary erythropoiesis: involvement of epor, gr, c-kit and bmp4 signaling" in Haematologica, 98 (2013):418-418,
https://hdl.handle.net/21.15107/rcub_rimi_520 .

TY  - JOUR
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Vignjević, Sanja
AU  - Mitrović, Olivera
AU  - Đikić, Dragoslava
AU  - Koko, Vesna
AU  - Čokić, Vladan
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/523
AB  - Aims: We showed previously that the acute effect of ethanol on intestinal immunoglobulin A (IgA) expression might be mediated by endogenous nitric oxide (NO). To extend these findings, this study was designed to investigate a possible role of neuronal NO synthase (nNOS) in the observed phenomenon, using 7-nitroindazole (7-NI), a selective inhibitor of its activity. Methods: Adult male Wistar rats were treated with: (a) ethanol (4 g/kg, intraperitoneally, i.p.), (b) 7-NI (25 mg/kg, i.p.) followed by ethanol (4 g/kg, i.p.) 30 min later and (c) 7-NI (25 mg/kg, i.p.) followed by saline 30 min later. Untreated rats were used as controls. The concentrations of serum and intestinal IgA were measured by enzyme-linked immunosorbent assay, while the expression of nNOS was determined using western blot and immunohistochemistry. Results: Acute ethanol treatment significantly increased the concentration of IgA in the ileal extracts, whereas it decreased its serum level. Inhibition of nNOS activity by 7-NI abolished this action of alcohol on IgA. Additionally, western blot analysis revealed that the acute alcohol administration induced an increase in the expression of intestinal nNOS. Furthermore, nNOS-immunoreactive cells, observed within the lamina propria of small intestine, were numerous in ethanol-treated rats. Conclusion: Taken together, these results extended our previous findings suggesting that nNOS mediates the acute effect of ethanol on IgA and supported an immunomodulatory role of this enzyme isoform.
PB  - Oxford Univ Press, Oxford
T2  - Alcohol & Alcoholism
T1  - Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA
EP  - 58
IS  - 1
SP  - 53
VL  - 48
DO  - 10.1093/alcalc/ags110
ER  - 

@article{
author = "Budeč, Mirela and Marković, Dragana and Vignjević, Sanja and Mitrović, Olivera and Đikić, Dragoslava and Koko, Vesna and Čokić, Vladan",
year = "2013",
abstract = "Aims: We showed previously that the acute effect of ethanol on intestinal immunoglobulin A (IgA) expression might be mediated by endogenous nitric oxide (NO). To extend these findings, this study was designed to investigate a possible role of neuronal NO synthase (nNOS) in the observed phenomenon, using 7-nitroindazole (7-NI), a selective inhibitor of its activity. Methods: Adult male Wistar rats were treated with: (a) ethanol (4 g/kg, intraperitoneally, i.p.), (b) 7-NI (25 mg/kg, i.p.) followed by ethanol (4 g/kg, i.p.) 30 min later and (c) 7-NI (25 mg/kg, i.p.) followed by saline 30 min later. Untreated rats were used as controls. The concentrations of serum and intestinal IgA were measured by enzyme-linked immunosorbent assay, while the expression of nNOS was determined using western blot and immunohistochemistry. Results: Acute ethanol treatment significantly increased the concentration of IgA in the ileal extracts, whereas it decreased its serum level. Inhibition of nNOS activity by 7-NI abolished this action of alcohol on IgA. Additionally, western blot analysis revealed that the acute alcohol administration induced an increase in the expression of intestinal nNOS. Furthermore, nNOS-immunoreactive cells, observed within the lamina propria of small intestine, were numerous in ethanol-treated rats. Conclusion: Taken together, these results extended our previous findings suggesting that nNOS mediates the acute effect of ethanol on IgA and supported an immunomodulatory role of this enzyme isoform.",
publisher = "Oxford Univ Press, Oxford",
journal = "Alcohol & Alcoholism",
title = "Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA",
pages = "58-53",
number = "1",
volume = "48",
doi = "10.1093/alcalc/ags110"
}

Budeč, M., Marković, D., Vignjević, S., Mitrović, O., Đikić, D., Koko, V.,& Čokić, V.. (2013). Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA. in Alcohol & Alcoholism
Oxford Univ Press, Oxford., 48(1), 53-58.
https://doi.org/10.1093/alcalc/ags110

Budeč M, Marković D, Vignjević S, Mitrović O, Đikić D, Koko V, Čokić V. Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA. in Alcohol & Alcoholism. 2013;48(1):53-58.
doi:10.1093/alcalc/ags110 .

Budeč, Mirela, Marković, Dragana, Vignjević, Sanja, Mitrović, Olivera, Đikić, Dragoslava, Koko, Vesna, Čokić, Vladan, "Neuronal Nitric Oxide Synthase Mediates the Effect of Ethanol on IgA" in Alcohol & Alcoholism, 48, no. 1 (2013):53-58,
https://doi.org/10.1093/alcalc/ags110 . .

TY  - JOUR
AU  - Petrovic-Kosanović, Dragana
AU  - Čakić-Milošević, Maja
AU  - Budeč, Mirela
AU  - Koko, Vesna
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/414
AB  - Isolated rat adrenal medulla was analyzed by light and electron microscope after an acute (60 min) exposure to high ambient temperature (38A degrees C). Under these conditions there was a significant rise in plasma adrenaline and noradrenaline. Stereological investigation by light microscopy showed a significant decrease in volume density of cells and an increase in the interstitium. At the ultrastructural level, the profile area of cells, nuclei and cytoplasm of adrenaline cells were significantly decreased. After the heat stress numbers of resting granules in adre naline and noradrenaline cells were significantly reduced, while the numbers of altered granules and empty containers in both types of adrenomedullar cells were significantly increased.
PB  - Sciendo, Warsaw
T2  - Central European Journal of Biology
T1  - Effect of acute heat stress on rat adrenal medulla - a morphological and ultrastructural study
EP  - 610
IS  - 4
SP  - 603
VL  - 7
DO  - 10.2478/s11535-012-0044-1
ER  - 

@article{
author = "Petrovic-Kosanović, Dragana and Čakić-Milošević, Maja and Budeč, Mirela and Koko, Vesna",
year = "2012",
abstract = "Isolated rat adrenal medulla was analyzed by light and electron microscope after an acute (60 min) exposure to high ambient temperature (38A degrees C). Under these conditions there was a significant rise in plasma adrenaline and noradrenaline. Stereological investigation by light microscopy showed a significant decrease in volume density of cells and an increase in the interstitium. At the ultrastructural level, the profile area of cells, nuclei and cytoplasm of adrenaline cells were significantly decreased. After the heat stress numbers of resting granules in adre naline and noradrenaline cells were significantly reduced, while the numbers of altered granules and empty containers in both types of adrenomedullar cells were significantly increased.",
publisher = "Sciendo, Warsaw",
journal = "Central European Journal of Biology",
title = "Effect of acute heat stress on rat adrenal medulla - a morphological and ultrastructural study",
pages = "610-603",
number = "4",
volume = "7",
doi = "10.2478/s11535-012-0044-1"
}

Petrovic-Kosanović, D., Čakić-Milošević, M., Budeč, M.,& Koko, V.. (2012). Effect of acute heat stress on rat adrenal medulla - a morphological and ultrastructural study. in Central European Journal of Biology
Sciendo, Warsaw., 7(4), 603-610.
https://doi.org/10.2478/s11535-012-0044-1

Petrovic-Kosanović D, Čakić-Milošević M, Budeč M, Koko V. Effect of acute heat stress on rat adrenal medulla - a morphological and ultrastructural study. in Central European Journal of Biology. 2012;7(4):603-610.
doi:10.2478/s11535-012-0044-1 .

Petrovic-Kosanović, Dragana, Čakić-Milošević, Maja, Budeč, Mirela, Koko, Vesna, "Effect of acute heat stress on rat adrenal medulla - a morphological and ultrastructural study" in Central European Journal of Biology, 7, no. 4 (2012):603-610,
https://doi.org/10.2478/s11535-012-0044-1 . .

TY  - JOUR
AU  - Đikić, Dragoslava
AU  - Budeč, Mirela
AU  - Vranješ-Đurić, Sanja
AU  - Koko, Vesna
AU  - Vignjević, Sanja
AU  - Mitrović, Olivera
PY  - 2011
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/335
AB  - Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.
PB  - Oxford Univ Press, Oxford
T2  - Alcohol & Alcoholism
T1  - The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide
EP  - 528
IS  - 5
SP  - 523
VL  - 46
DO  - 10.1093/alcalc/agr054
ER  - 

@article{
author = "Đikić, Dragoslava and Budeč, Mirela and Vranješ-Đurić, Sanja and Koko, Vesna and Vignjević, Sanja and Mitrović, Olivera",
year = "2011",
abstract = "Aims: The present study was designed to investigate a possible role of endogenous nitric oxide (NO) in the adrenal response to an acute alcohol administration in female rats. To this end, N(omega)-nitro-l-arginine-methyl ester (l-NAME), a competitive inhibitor of all isoforms of NO synthase, was used. Methods: Adult female Wistar rats showing diestrus Day 1 were treated with: (a) ethanol (2 or 4 g/kg, intraperitoneally); (b) l-NAME (30 or 50 mg/kg, subcutaneously) followed by either ethanol or saline 3 h later. Untreated and saline-injected rats were used as controls. The animals were killed 30 min after last injection. Adrenal cortex was analyzed morphometrically, and plasma levels of adrenocorticotropic hormone (ACTH) and serum concentrations of corticosterone were determined. Results: Acute ethanol treatment enhanced the levels of ACTH and corticosterone in a dose-dependent manner. Stereological analysis revealed that acute alcohol administration induced a significant increase in absolute volume of the cortex and the zona fasciculata (ZF). In addition, ethanol at a dose of 4 g/kg increased volume density and length of the capillaries in the ZF. However, other stereological parameters were unaffected by alcohol exposure. Pretreatment with both doses of l-NAME had no effect on ethanol-induced changes. Conclusion: Obtained findings indicate that acute ethanol treatment stimulates the activity of the adrenal cortex and that this effect is not mediated by endogenous NO in female rats under these experimental conditions.",
publisher = "Oxford Univ Press, Oxford",
journal = "Alcohol & Alcoholism",
title = "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide",
pages = "528-523",
number = "5",
volume = "46",
doi = "10.1093/alcalc/agr054"
}

Đikić, D., Budeč, M., Vranješ-Đurić, S., Koko, V., Vignjević, S.,& Mitrović, O.. (2011). The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol & Alcoholism
Oxford Univ Press, Oxford., 46(5), 523-528.
https://doi.org/10.1093/alcalc/agr054

Đikić D, Budeč M, Vranješ-Đurić S, Koko V, Vignjević S, Mitrović O. The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide. in Alcohol & Alcoholism. 2011;46(5):523-528.
doi:10.1093/alcalc/agr054 .

Đikić, Dragoslava, Budeč, Mirela, Vranješ-Đurić, Sanja, Koko, Vesna, Vignjević, Sanja, Mitrović, Olivera, "The Acute Effect of Ethanol on Adrenal Cortex in Female Rats-Possible Role of Nitric Oxide" in Alcohol & Alcoholism, 46, no. 5 (2011):523-528,
https://doi.org/10.1093/alcalc/agr054 . .

TY  - JOUR
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Mitrović, Olivera
AU  - Drndarević, Neda
AU  - Koko, Vesna
AU  - Todorović, Vera
PY  - 2009
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/219
AB  - Objective: Nitric oxide (NO) is known as a regulator of inflammation and immunity. The purpose of this study was to investigate the influence of this signal molecule on the rat immunoglobulin A (IgA) system using N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of NO synthase. Methods: The experiments were performed on adult female Wistar rats showing diestrus day 1 that were treated with L-NAME (30 or 50 mg/kg, s.c.). Untreated and saline-injected animals were used as controls. The rats were sacrificed 3 h following L-NAME or saline administration. The concentration of IgA in serum and intestinal extracts was determined by a sandwich enzyme-linked immunosorbent assay. The number of IgA-expressing cells per area unit of Peyer's patches and the intestinal lamina propria was evaluated using stereological analysis. Results: The results showed that L-NAME decreased the level of IgA in serum and elevated its concentration in intestinal extracts. Additionally, the increased number of IgA+ cells was found in the intestinal lamina propria in both experimental groups. Conclusion: Obtained findings imply that endogenous NO may modulate the IgA system in the rat. Copyright (C) 2009 S. Karger AG, Basel
PB  - Karger, Basel
T2  - Neuroimmunomodulation
T1  - Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A
EP  - 161
IS  - 3
SP  - 155
VL  - 16
DO  - 10.1159/000204228
ER  - 

@article{
author = "Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Mitrović, Olivera and Drndarević, Neda and Koko, Vesna and Todorović, Vera",
year = "2009",
abstract = "Objective: Nitric oxide (NO) is known as a regulator of inflammation and immunity. The purpose of this study was to investigate the influence of this signal molecule on the rat immunoglobulin A (IgA) system using N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of NO synthase. Methods: The experiments were performed on adult female Wistar rats showing diestrus day 1 that were treated with L-NAME (30 or 50 mg/kg, s.c.). Untreated and saline-injected animals were used as controls. The rats were sacrificed 3 h following L-NAME or saline administration. The concentration of IgA in serum and intestinal extracts was determined by a sandwich enzyme-linked immunosorbent assay. The number of IgA-expressing cells per area unit of Peyer's patches and the intestinal lamina propria was evaluated using stereological analysis. Results: The results showed that L-NAME decreased the level of IgA in serum and elevated its concentration in intestinal extracts. Additionally, the increased number of IgA+ cells was found in the intestinal lamina propria in both experimental groups. Conclusion: Obtained findings imply that endogenous NO may modulate the IgA system in the rat. Copyright (C) 2009 S. Karger AG, Basel",
publisher = "Karger, Basel",
journal = "Neuroimmunomodulation",
title = "Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A",
pages = "161-155",
number = "3",
volume = "16",
doi = "10.1159/000204228"
}

Budeč, M., Marković, D., Đikić, D., Mitrović, O., Drndarević, N., Koko, V.,& Todorović, V.. (2009). Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A. in Neuroimmunomodulation
Karger, Basel., 16(3), 155-161.
https://doi.org/10.1159/000204228

Budeč M, Marković D, Đikić D, Mitrović O, Drndarević N, Koko V, Todorović V. Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A. in Neuroimmunomodulation. 2009;16(3):155-161.
doi:10.1159/000204228 .

Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Mitrović, Olivera, Drndarević, Neda, Koko, Vesna, Todorović, Vera, "Blockade of Nitric Oxide Synthesis Modulates Rat Immunoglobulin A" in Neuroimmunomodulation, 16, no. 3 (2009):155-161,
https://doi.org/10.1159/000204228 . .

TY  - CONF
AU  - Budeč, Mirela
AU  - Marković, Dragana
AU  - Đikić, Dragoslava
AU  - Koko, Vesna
AU  - Todorović, Vera
PY  - 2008
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/198
PB  - Elsevier Science Bv, Amsterdam
C3  - Journal of Neuroimmunology
T1  - Blockade of nitric oxide formation modulates intestinal IgA
EP  - 172
IS  - 2
SP  - 172
VL  - 197
UR  - https://hdl.handle.net/21.15107/rcub_rimi_198
ER  - 

@conference{
author = "Budeč, Mirela and Marković, Dragana and Đikić, Dragoslava and Koko, Vesna and Todorović, Vera",
year = "2008",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Blockade of nitric oxide formation modulates intestinal IgA",
pages = "172-172",
number = "2",
volume = "197",
url = "https://hdl.handle.net/21.15107/rcub_rimi_198"
}

Budeč, M., Marković, D., Đikić, D., Koko, V.,& Todorović, V.. (2008). Blockade of nitric oxide formation modulates intestinal IgA. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 197(2), 172-172.
https://hdl.handle.net/21.15107/rcub_rimi_198

Budeč M, Marković D, Đikić D, Koko V, Todorović V. Blockade of nitric oxide formation modulates intestinal IgA. in Journal of Neuroimmunology. 2008;197(2):172-172.
https://hdl.handle.net/21.15107/rcub_rimi_198 .

Budeč, Mirela, Marković, Dragana, Đikić, Dragoslava, Koko, Vesna, Todorović, Vera, "Blockade of nitric oxide formation modulates intestinal IgA" in Journal of Neuroimmunology, 197, no. 2 (2008):172-172,
https://hdl.handle.net/21.15107/rcub_rimi_198 .

TY  - JOUR
AU  - Budeč, Mirela
AU  - Koko, Vesna
AU  - Todorović, Vera
AU  - Marković, Dragana
AU  - Poštić, Marija M.
AU  - Drndarević, Neda
AU  - Spasić, Anđelka
AU  - Mitrović, Olivera
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/170
AB  - The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.
PB  - Elsevier Science Bv, Amsterdam
T2  - International Immunopharmacology
T1  - Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat
EP  - 863
IS  - 6
SP  - 858
VL  - 7
DO  - 10.1016/j.intimp.2007.02.010
ER  - 

@article{
author = "Budeč, Mirela and Koko, Vesna and Todorović, Vera and Marković, Dragana and Poštić, Marija M. and Drndarević, Neda and Spasić, Anđelka and Mitrović, Olivera",
year = "2007",
abstract = "The purpose of this study was to investigate the possible mechanism of acute effect of ethanol on IgA expression in rat intestine. To this end, adult female Wistar rats showing diestrus day I were treated with (a) ethanol (2 or 4 g/kg, i.p.); (b) N omega-nitro-L-arginine-methyl ester (L-NAME), which inhibits the activity of all isoforms of nitric oxide synthase, (30 mg/kg, s.c.) followed by ethanol 3 h later-, and (C) L-NAME (30 mg/kg, s.c.) followed by saline 3 h later. Saline-injected and untreated rats were used as controls. The animals were sacrificed 0.5 h after ethanol administration. Intestinal expression of IgA was evaluated by both immunohistochemistry and Western immunoblotting. Morphometric analysis showed that acute ethanol treatment increased the number of IgA-immunoreactive cells in a dose-dependent manner. Pretreatment with L-NAME abolished this action of alcohol. Injection of L-NAME followed by saline had no influence on the number of IgA+cells. The results, obtained by Western immunoblotting, paralleled our immunohistochemical findings. Taken together, these data suggest that acute effect of ethanol on intestinal IgA might be mediated by endogenous nitric oxide.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "International Immunopharmacology",
title = "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat",
pages = "863-858",
number = "6",
volume = "7",
doi = "10.1016/j.intimp.2007.02.010"
}

Budeč, M., Koko, V., Todorović, V., Marković, D., Poštić, M. M., Drndarević, N., Spasić, A.,& Mitrović, O.. (2007). Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat. in International Immunopharmacology
Elsevier Science Bv, Amsterdam., 7(6), 858-863.
https://doi.org/10.1016/j.intimp.2007.02.010

Budeč M, Koko V, Todorović V, Marković D, Poštić MM, Drndarević N, Spasić A, Mitrović O. Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat. in International Immunopharmacology. 2007;7(6):858-863.
doi:10.1016/j.intimp.2007.02.010 .

Budeč, Mirela, Koko, Vesna, Todorović, Vera, Marković, Dragana, Poštić, Marija M., Drndarević, Neda, Spasić, Anđelka, Mitrović, Olivera, "Possible mechanism of acute effect of ethanol on intestinal IgA expression in rat" in International Immunopharmacology, 7, no. 6 (2007):858-863,
https://doi.org/10.1016/j.intimp.2007.02.010 . .

TY  - JOUR
AU  - Milovanović, T
AU  - Budeč, Mirela
AU  - Balint-Perić, L
AU  - Koko, Vesna
AU  - Todorović, Vera
PY  - 2003
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/88
AB  - Objective: The purpose of this study was to investigate the effect of a single dose of ethanol on rat adrenal cortex and to determine whether the estrous cycle can influence this effect of ethanol. Method: Adult female Wistar rats showing proestrus or diestrus Day I (n = 12) were treated intraperitoneally with ethanol (4 g/kg body weight). Untreated (n = 15) and saline-injected (n = 14) rats were used as controls. The animals were sacrificed by decapitation 0.5 hour after ethanol administration. Stereological analysis was performed on paraffin sections of adrenal glands stained with AZAN, and the following parameters were determined: absolute volume of the zona glomerulosa, the zona fasciculata and the zona reticularis, numerical density, volume and the mean diameter of adrenocortical cells and of their nuclei, and diameter and length of capillaries. Results: The diameter and volume of adrenocortical cells in the zona fasciculata and the zona reticularis were significantly increased by acute ethanol treatment at proestrus. In the same group of animals, a single dose of ethanol induced significant decrease in numerical density of adrenocortical cells and of their nuclei in all three zones. Increased length of capillaries of the zona fasciculata as well as enhanced level of serum corticosterone was found in ethanol-treated rats at both phases of the estrous cycle, proestrus and diestrus Day 1. Conclusions: The obtained results indicate that a single dose of ethanol activates adrenal cortex in female rats and that the effect is more pronounced on morphometric parameters at proestrus.
PB  - Alcohol Res Documentation Inc Cent Alcohol Stud Rutgers Univ, Piscataway
T2  - Journal of Studies on Alcohol
T1  - Effects of acute administration of ethanol on the rat adrenal cortex
EP  - 668
IS  - 5
SP  - 662
VL  - 64
DO  - 10.15288/jsa.2003.64.662
ER  - 

@article{
author = "Milovanović, T and Budeč, Mirela and Balint-Perić, L and Koko, Vesna and Todorović, Vera",
year = "2003",
abstract = "Objective: The purpose of this study was to investigate the effect of a single dose of ethanol on rat adrenal cortex and to determine whether the estrous cycle can influence this effect of ethanol. Method: Adult female Wistar rats showing proestrus or diestrus Day I (n = 12) were treated intraperitoneally with ethanol (4 g/kg body weight). Untreated (n = 15) and saline-injected (n = 14) rats were used as controls. The animals were sacrificed by decapitation 0.5 hour after ethanol administration. Stereological analysis was performed on paraffin sections of adrenal glands stained with AZAN, and the following parameters were determined: absolute volume of the zona glomerulosa, the zona fasciculata and the zona reticularis, numerical density, volume and the mean diameter of adrenocortical cells and of their nuclei, and diameter and length of capillaries. Results: The diameter and volume of adrenocortical cells in the zona fasciculata and the zona reticularis were significantly increased by acute ethanol treatment at proestrus. In the same group of animals, a single dose of ethanol induced significant decrease in numerical density of adrenocortical cells and of their nuclei in all three zones. Increased length of capillaries of the zona fasciculata as well as enhanced level of serum corticosterone was found in ethanol-treated rats at both phases of the estrous cycle, proestrus and diestrus Day 1. Conclusions: The obtained results indicate that a single dose of ethanol activates adrenal cortex in female rats and that the effect is more pronounced on morphometric parameters at proestrus.",
publisher = "Alcohol Res Documentation Inc Cent Alcohol Stud Rutgers Univ, Piscataway",
journal = "Journal of Studies on Alcohol",
title = "Effects of acute administration of ethanol on the rat adrenal cortex",
pages = "668-662",
number = "5",
volume = "64",
doi = "10.15288/jsa.2003.64.662"
}

Milovanović, T., Budeč, M., Balint-Perić, L., Koko, V.,& Todorović, V.. (2003). Effects of acute administration of ethanol on the rat adrenal cortex. in Journal of Studies on Alcohol
Alcohol Res Documentation Inc Cent Alcohol Stud Rutgers Univ, Piscataway., 64(5), 662-668.
https://doi.org/10.15288/jsa.2003.64.662

Milovanović T, Budeč M, Balint-Perić L, Koko V, Todorović V. Effects of acute administration of ethanol on the rat adrenal cortex. in Journal of Studies on Alcohol. 2003;64(5):662-668.
doi:10.15288/jsa.2003.64.662 .

Milovanović, T, Budeč, Mirela, Balint-Perić, L, Koko, Vesna, Todorović, Vera, "Effects of acute administration of ethanol on the rat adrenal cortex" in Journal of Studies on Alcohol, 64, no. 5 (2003):662-668,
https://doi.org/10.15288/jsa.2003.64.662 . .

TY  - JOUR
AU  - Todorović, Vera
AU  - Koko, Vesna
AU  - Petakov, Marijana
AU  - Jovčić, Gordana
AU  - Stojanović, N.
AU  - Bugarski, Diana
AU  - Perić, P
PY  - 1999
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/52
AB  - The specific influence of malnutrition on the pathophysiologic changes induced by chronic alcoholism is controversial. In an attempt to determine and demarcate the effects of protein malnutrition from those produced by alcoholism and to evaluate the precise effect of alcohol per se on cytochemical and ultrastructural properties of rat polymorphonuclear neutrophil (PMN) granules, we investigated the influence of chronic protein malnutrition or chronic alcoholism alone and in combination, in rats, After a 4 month experimental period various PMN properties, such as cytochemical, morphometrical and ultrastructural, as well, as neutrophil functions were studied. It was found that the degree of damage of PMNs induced either by ethanol or protein malnutrition alone was similar whereas their combination led to worsening of all markers of PMN functional ability. Ultrastructural changes of neutrophil granules including reduction, redistribution and atypical accumulation as well as appearance of autophagic vacuoles, confirmed their alteration which was emphasised by the additive pathophysiological interaction of alcoholism and chronic hypoprotein malnutrition.
PB  - Wolters Kluwer Medknow Publications, Mumbai
T2  - Indian Journal of Medical Research
T1  - Cytochemical & ultrastructural alteration of cytoplasmic granules of rat peripheral blood neutrophils induced by chronic alcoholism & malnutrition
EP  - 114
SP  - 105
VL  - 109
UR  - https://hdl.handle.net/21.15107/rcub_rimi_52
ER  - 

@article{
author = "Todorović, Vera and Koko, Vesna and Petakov, Marijana and Jovčić, Gordana and Stojanović, N. and Bugarski, Diana and Perić, P",
year = "1999",
abstract = "The specific influence of malnutrition on the pathophysiologic changes induced by chronic alcoholism is controversial. In an attempt to determine and demarcate the effects of protein malnutrition from those produced by alcoholism and to evaluate the precise effect of alcohol per se on cytochemical and ultrastructural properties of rat polymorphonuclear neutrophil (PMN) granules, we investigated the influence of chronic protein malnutrition or chronic alcoholism alone and in combination, in rats, After a 4 month experimental period various PMN properties, such as cytochemical, morphometrical and ultrastructural, as well, as neutrophil functions were studied. It was found that the degree of damage of PMNs induced either by ethanol or protein malnutrition alone was similar whereas their combination led to worsening of all markers of PMN functional ability. Ultrastructural changes of neutrophil granules including reduction, redistribution and atypical accumulation as well as appearance of autophagic vacuoles, confirmed their alteration which was emphasised by the additive pathophysiological interaction of alcoholism and chronic hypoprotein malnutrition.",
publisher = "Wolters Kluwer Medknow Publications, Mumbai",
journal = "Indian Journal of Medical Research",
title = "Cytochemical & ultrastructural alteration of cytoplasmic granules of rat peripheral blood neutrophils induced by chronic alcoholism & malnutrition",
pages = "114-105",
volume = "109",
url = "https://hdl.handle.net/21.15107/rcub_rimi_52"
}

Todorović, V., Koko, V., Petakov, M., Jovčić, G., Stojanović, N., Bugarski, D.,& Perić, P.. (1999). Cytochemical & ultrastructural alteration of cytoplasmic granules of rat peripheral blood neutrophils induced by chronic alcoholism & malnutrition. in Indian Journal of Medical Research
Wolters Kluwer Medknow Publications, Mumbai., 109, 105-114.
https://hdl.handle.net/21.15107/rcub_rimi_52

Todorović V, Koko V, Petakov M, Jovčić G, Stojanović N, Bugarski D, Perić P. Cytochemical & ultrastructural alteration of cytoplasmic granules of rat peripheral blood neutrophils induced by chronic alcoholism & malnutrition. in Indian Journal of Medical Research. 1999;109:105-114.
https://hdl.handle.net/21.15107/rcub_rimi_52 .

Todorović, Vera, Koko, Vesna, Petakov, Marijana, Jovčić, Gordana, Stojanović, N., Bugarski, Diana, Perić, P, "Cytochemical & ultrastructural alteration of cytoplasmic granules of rat peripheral blood neutrophils induced by chronic alcoholism & malnutrition" in Indian Journal of Medical Research, 109 (1999):105-114,
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