TY  - JOUR
AU  - Janjić, Filip
AU  - Beletić, Anđelo
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Diklić, Miloš
AU  - Andrić, Jelena Francuski
AU  - Radonjić, Vladimir
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1214
AB  - The highest number of acute Babesia canis cases in dogs is recorded over the February-May (Feb-May) period, which also represents the optimal climate conditions for tick activity in Belgrade, Serbia. A possibility that the acute phase response is more intense in dogs developing the disease in the Feb-May period compared with the response in other time periods of the year was tested. A total of 63 client-owned dogs with acute B. canis infection were enrolled and the routine hematology and biochemistry parameters-serum amyloid A (SAA), IgG against B. canis, level of parasitemia, ceruloplasmin (CER), paraoxonase-1 (PON-1), and fibrinogen-were measured. Acute phase indexes (API) were calculated as (SAA×CER)/(Iron×PON-1) and (SAA×CER)/(Albumin×Iron). Statistics included Kruskal-Wallis test and logistic regression analysis. The results showed that in the Feb-May period, the following parameters were lower: creatinine, albumin, iron, and level of parasitemia. Furthermore, increased API values were more probable in the Feb-May than in the other periods. Together, higher acute phase response intensity and presumptive hemodilution in the Feb-May period indicate a more acute course of B. canis infection than in other time periods of the year.
PB  - Springer
T2  - International Journal of Biometeorology
T1  - Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis
EP  - 698
IS  - 4
SP  - 691
VL  - 66
DO  - 10.1007/s00484-021-02228-4
ER  - 

@article{
author = "Janjić, Filip and Beletić, Anđelo and Radaković, Milena and Spariosu, Kristina and Diklić, Miloš and Andrić, Jelena Francuski and Radonjić, Vladimir and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2022",
abstract = "The highest number of acute Babesia canis cases in dogs is recorded over the February-May (Feb-May) period, which also represents the optimal climate conditions for tick activity in Belgrade, Serbia. A possibility that the acute phase response is more intense in dogs developing the disease in the Feb-May period compared with the response in other time periods of the year was tested. A total of 63 client-owned dogs with acute B. canis infection were enrolled and the routine hematology and biochemistry parameters-serum amyloid A (SAA), IgG against B. canis, level of parasitemia, ceruloplasmin (CER), paraoxonase-1 (PON-1), and fibrinogen-were measured. Acute phase indexes (API) were calculated as (SAA×CER)/(Iron×PON-1) and (SAA×CER)/(Albumin×Iron). Statistics included Kruskal-Wallis test and logistic regression analysis. The results showed that in the Feb-May period, the following parameters were lower: creatinine, albumin, iron, and level of parasitemia. Furthermore, increased API values were more probable in the Feb-May than in the other periods. Together, higher acute phase response intensity and presumptive hemodilution in the Feb-May period indicate a more acute course of B. canis infection than in other time periods of the year.",
publisher = "Springer",
journal = "International Journal of Biometeorology",
title = "Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis",
pages = "698-691",
number = "4",
volume = "66",
doi = "10.1007/s00484-021-02228-4"
}

Janjić, F., Beletić, A., Radaković, M., Spariosu, K., Diklić, M., Andrić, J. F., Radonjić, V., Ajtić, J.,& Kovačević-Filipović, M.. (2022). Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis. in International Journal of Biometeorology
Springer., 66(4), 691-698.
https://doi.org/10.1007/s00484-021-02228-4

Janjić F, Beletić A, Radaković M, Spariosu K, Diklić M, Andrić JF, Radonjić V, Ajtić J, Kovačević-Filipović M. Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis. in International Journal of Biometeorology. 2022;66(4):691-698.
doi:10.1007/s00484-021-02228-4 .

Janjić, Filip, Beletić, Anđelo, Radaković, Milena, Spariosu, Kristina, Diklić, Miloš, Andrić, Jelena Francuski, Radonjić, Vladimir, Ajtić, Jelena, Kovačević-Filipović, Milica, "Seasonal differences in the intensity of acute phase response in dogs infected with Babesia canis" in International Journal of Biometeorology, 66, no. 4 (2022):691-698,
https://doi.org/10.1007/s00484-021-02228-4 . .

Potkonjak, A., Savić, S., Spasojević-Kosić, L., Tasić-Otašević, S., Tomanović, S.,& Kovačević-Filipović, M.. (2020). Consensus statement on the epidemiological situation and expected frequency of canine vector-borne diseases in Serbia. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(2), 211-215.
https://doi.org/10.2298/VETGL201124016P

Potkonjak A, Savić S, Spasojević-Kosić L, Tasić-Otašević S, Tomanović S, Kovačević-Filipović M. Consensus statement on the epidemiological situation and expected frequency of canine vector-borne diseases in Serbia. in Veterinarski glasnik. 2020;74(2):211-215.
doi:10.2298/VETGL201124016P .

Potkonjak, Aleksandar, Savić, Sara, Spasojević-Kosić, Ljubica, Tasić-Otašević, Suzana, Tomanović, Snežana, Kovačević-Filipović, Milica, "Consensus statement on the epidemiological situation and expected frequency of canine vector-borne diseases in Serbia" in Veterinarski glasnik, 74, no. 2 (2020):211-215,
https://doi.org/10.2298/VETGL201124016P . .

TY  - JOUR
AU  - Janjić, Filip
AU  - Sarvan, Darko
AU  - Tomanović, Snežana
AU  - Cuk, Jelena
AU  - Krstić, Vanja
AU  - Radonjić, Vladimir
AU  - Kovačević-Filipović, Milica
AU  - Ajtić, Jelena
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/908
AB  - Belgrade, the capital of the Republic of Serbia, is an endemic location for canine babesiosis caused by Babesia canis. This tick-borne disease occurs seasonally in regions with moderate continental climate. However, recent clinical data show that 1) some cases of canine babesiosis have been recorded during the winter, and 2) canine babesiosis is spreading to the northern parts of Europe with a colder climate, which is a region previously free of this disease. Our study investigates the occurrence of canine babesiosis in different seasons over 2013-2016, and explores a short-term link between meteorological parameters and number of diagnosed cases of canine babesiosis in Belgrade. It also looks into possible long-term correlations that span one year before the onset of the disease. Based on 872 recorded cases over four years, our results show a bimodal seasonal distribution of canine babesiosis, with a pronounced peak in the spring, and a less conspicuous one in the autumn. Throughout the year, even over the coldest and warmest periods, there is a broad range of temperatures and relative humidifies when the disease is recorded. Over one year prior to the spring and autumn onset of the disease, we found a noticeable impact of temperature and relative humidity, and to a lesser extent, of atmospheric pressure and cloud cover, on the number of diagnosed cases. These findings imply short-term and long-term relationships between occurrence of acute canine babesiosis and certain meteorological parameters, and they open further questions that need to be investigated in order to understand the epidemiology of this disease.
PB  - Elsevier Gmbh, Munich
T2  - Ticks & Tick-Borne Diseases
T1  - A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia
IS  - 6
VL  - 10
DO  - 10.1016/j.ttbdis.2019.101273
ER  - 

@article{
author = "Janjić, Filip and Sarvan, Darko and Tomanović, Snežana and Cuk, Jelena and Krstić, Vanja and Radonjić, Vladimir and Kovačević-Filipović, Milica and Ajtić, Jelena",
year = "2019",
abstract = "Belgrade, the capital of the Republic of Serbia, is an endemic location for canine babesiosis caused by Babesia canis. This tick-borne disease occurs seasonally in regions with moderate continental climate. However, recent clinical data show that 1) some cases of canine babesiosis have been recorded during the winter, and 2) canine babesiosis is spreading to the northern parts of Europe with a colder climate, which is a region previously free of this disease. Our study investigates the occurrence of canine babesiosis in different seasons over 2013-2016, and explores a short-term link between meteorological parameters and number of diagnosed cases of canine babesiosis in Belgrade. It also looks into possible long-term correlations that span one year before the onset of the disease. Based on 872 recorded cases over four years, our results show a bimodal seasonal distribution of canine babesiosis, with a pronounced peak in the spring, and a less conspicuous one in the autumn. Throughout the year, even over the coldest and warmest periods, there is a broad range of temperatures and relative humidifies when the disease is recorded. Over one year prior to the spring and autumn onset of the disease, we found a noticeable impact of temperature and relative humidity, and to a lesser extent, of atmospheric pressure and cloud cover, on the number of diagnosed cases. These findings imply short-term and long-term relationships between occurrence of acute canine babesiosis and certain meteorological parameters, and they open further questions that need to be investigated in order to understand the epidemiology of this disease.",
publisher = "Elsevier Gmbh, Munich",
journal = "Ticks & Tick-Borne Diseases",
title = "A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia",
number = "6",
volume = "10",
doi = "10.1016/j.ttbdis.2019.101273"
}

Janjić, F., Sarvan, D., Tomanović, S., Cuk, J., Krstić, V., Radonjić, V., Kovačević-Filipović, M.,& Ajtić, J.. (2019). A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia. in Ticks & Tick-Borne Diseases
Elsevier Gmbh, Munich., 10(6).
https://doi.org/10.1016/j.ttbdis.2019.101273

Janjić F, Sarvan D, Tomanović S, Cuk J, Krstić V, Radonjić V, Kovačević-Filipović M, Ajtić J. A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia. in Ticks & Tick-Borne Diseases. 2019;10(6).
doi:10.1016/j.ttbdis.2019.101273 .

Janjić, Filip, Sarvan, Darko, Tomanović, Snežana, Cuk, Jelena, Krstić, Vanja, Radonjić, Vladimir, Kovačević-Filipović, Milica, Ajtić, Jelena, "A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia" in Ticks & Tick-Borne Diseases, 10, no. 6 (2019),
https://doi.org/10.1016/j.ttbdis.2019.101273 . .

TY  - JOUR
AU  - Martačić, Jasmina
AU  - Kovačević-Filipović, Milica
AU  - Borozan, Sunčica
AU  - Cvetković, Zorica
AU  - Popović, Tamara B.
AU  - Arsić, Aleksandra
AU  - Takić, Marija
AU  - Vučić, Vesna M.
AU  - Glibetić, Marija
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/866
AB  - Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in comparison to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro.
PB  - Springer Heidelberg, Heidelberg
T2  - Clinical Oral Investigations
T1  - N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro
EP  - 2903
IS  - 8
SP  - 2897
VL  - 22
DO  - 10.1007/s00784-018-2377-2
ER  - 

@article{
author = "Martačić, Jasmina and Kovačević-Filipović, Milica and Borozan, Sunčica and Cvetković, Zorica and Popović, Tamara B. and Arsić, Aleksandra and Takić, Marija and Vučić, Vesna M. and Glibetić, Marija",
year = "2018",
abstract = "Objectives The aim of our study was to investigate whether N-acetyl-L-cysteine (NAC) could protect stem cells from exfoliated deciduous teeth (SHED) against oxidative damage, during in vitro cultivation, to preserve regenerative potential of these cells. Accordingly, we examined the potential of cell culture supplementation with NAC in prevention of lipid peroxidation, unfavorable changes of total lipids fatty acid composition, and the effects on the activity of antioxidant enzymes. Material and methods We analyzed the extent of oxidative damage in SHED after 48 h treatment with different NAC concentrations. Cellular lipid peroxidation was determined upon reaction with thiobarbituric acid. All enzyme activities were measured spectrophotometrically, based on published methods. Fatty acid methyl esters were analyzed by gas-liquid chromatography. Results Concentration of 0.1 mM NAC showed the most profound effects on SHED, significantly decreasing levels of lipid peroxidation in comparison to control. This dose also diminished the activities of antioxidant enzymes. Furthermore, NAC treatment significantly changed fatty acid composition of cells, reducing levels of oleic acid and monounsaturated fatty acids and increasing linoleic acid, n-6, and total polyunsaturated fatty acid (PUFA) proportions. Conclusion Low dose of NAC significantly decreased lipid peroxidation and altered fatty acid composition towards increasing PUFA. The reduced oxidative damage of cellular lipids could be strongly related to improved SHED survival in vitro.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Clinical Oral Investigations",
title = "N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro",
pages = "2903-2897",
number = "8",
volume = "22",
doi = "10.1007/s00784-018-2377-2"
}

Martačić, J., Kovačević-Filipović, M., Borozan, S., Cvetković, Z., Popović, T. B., Arsić, A., Takić, M., Vučić, V. M.,& Glibetić, M.. (2018). N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro. in Clinical Oral Investigations
Springer Heidelberg, Heidelberg., 22(8), 2897-2903.
https://doi.org/10.1007/s00784-018-2377-2

Martačić J, Kovačević-Filipović M, Borozan S, Cvetković Z, Popović TB, Arsić A, Takić M, Vučić VM, Glibetić M. N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro. in Clinical Oral Investigations. 2018;22(8):2897-2903.
doi:10.1007/s00784-018-2377-2 .

Martačić, Jasmina, Kovačević-Filipović, Milica, Borozan, Sunčica, Cvetković, Zorica, Popović, Tamara B., Arsić, Aleksandra, Takić, Marija, Vučić, Vesna M., Glibetić, Marija, "N-acetyl-L-cysteine protects dental tissue stem cells against oxidative stress in vitro" in Clinical Oral Investigations, 22, no. 8 (2018):2897-2903,
https://doi.org/10.1007/s00784-018-2377-2 . .

TY  - JOUR
AU  - Debeljak-Martačić, Jasmina
AU  - Borozan, Sunčica
AU  - Radovanović, Anita
AU  - Popadić, Dusan
AU  - Mojsilović, Slavko
AU  - Vučić, Vesna M.
AU  - Todorović, Vera
AU  - Kovačević-Filipović, Milica
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/693
AB  - Objective: Obtaining high number of stem cells is of interest for cell based therapies. N-Acetyl-L-cysteine (NAC) acts as a source of sulfhydryl groups and an anti-oxidative agent. The aim of this study was to test different NAC concentration on proliferation and differentiation of deciduous teeth dental pulp stem cells (DTSCs) in vitro as well as to define the possible underlining mechanism of its effect. Design: Number of viable, apoptotic and senescent DTSCs was determined after addition of NAC (0.1 mM, 1.0 mM, 2.0 mM). Also, cell cycle analysis, HIF1-alpha expression, LDH isoenzymes, superoxide-dismutase (SOD) and catalase (CAT) activity, sulfhydryl groups content, the level of lipids' and proteins' oxidative damage and differentiation capacity of NAC treated DTSCs was determined. Results: DTSCs expressed HIP-1 alpha in all conditions. The lowest NAC dose (0.1 mM) increased the number of DTSCs by one fifth comparing to the control, most likely stimulating entry of cells into S phase of cell cycle and enhancing the activity of LDH5 isoenzyme. The highest NAC dose (2 mM) inhibited DTSCs proliferation. Also, DTSCs had the lowest level of oxidative damage with 0.1 mM NAC. All tested NAC concentrations enhanced DTSCs osteo-chondrogenesis. Conclusion: The lowest NAC dose exerted significant positive effect on DTSCs proliferation as well as antioxidative protection creating beneficial environment for stem cells in vitro cultivation especially when their clinical use is important for stimulation of osteo-chondrogenesis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Archives of Oral Biology
T1  - N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells
EP  - 38
SP  - 32
VL  - 70
DO  - 10.1016/j.archoralbio.2016.06.002
ER  - 

@article{
author = "Debeljak-Martačić, Jasmina and Borozan, Sunčica and Radovanović, Anita and Popadić, Dusan and Mojsilović, Slavko and Vučić, Vesna M. and Todorović, Vera and Kovačević-Filipović, Milica",
year = "2016",
abstract = "Objective: Obtaining high number of stem cells is of interest for cell based therapies. N-Acetyl-L-cysteine (NAC) acts as a source of sulfhydryl groups and an anti-oxidative agent. The aim of this study was to test different NAC concentration on proliferation and differentiation of deciduous teeth dental pulp stem cells (DTSCs) in vitro as well as to define the possible underlining mechanism of its effect. Design: Number of viable, apoptotic and senescent DTSCs was determined after addition of NAC (0.1 mM, 1.0 mM, 2.0 mM). Also, cell cycle analysis, HIF1-alpha expression, LDH isoenzymes, superoxide-dismutase (SOD) and catalase (CAT) activity, sulfhydryl groups content, the level of lipids' and proteins' oxidative damage and differentiation capacity of NAC treated DTSCs was determined. Results: DTSCs expressed HIP-1 alpha in all conditions. The lowest NAC dose (0.1 mM) increased the number of DTSCs by one fifth comparing to the control, most likely stimulating entry of cells into S phase of cell cycle and enhancing the activity of LDH5 isoenzyme. The highest NAC dose (2 mM) inhibited DTSCs proliferation. Also, DTSCs had the lowest level of oxidative damage with 0.1 mM NAC. All tested NAC concentrations enhanced DTSCs osteo-chondrogenesis. Conclusion: The lowest NAC dose exerted significant positive effect on DTSCs proliferation as well as antioxidative protection creating beneficial environment for stem cells in vitro cultivation especially when their clinical use is important for stimulation of osteo-chondrogenesis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Archives of Oral Biology",
title = "N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells",
pages = "38-32",
volume = "70",
doi = "10.1016/j.archoralbio.2016.06.002"
}

Debeljak-Martačić, J., Borozan, S., Radovanović, A., Popadić, D., Mojsilović, S., Vučić, V. M., Todorović, V.,& Kovačević-Filipović, M.. (2016). N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells. in Archives of Oral Biology
Pergamon-Elsevier Science Ltd, Oxford., 70, 32-38.
https://doi.org/10.1016/j.archoralbio.2016.06.002

Debeljak-Martačić J, Borozan S, Radovanović A, Popadić D, Mojsilović S, Vučić VM, Todorović V, Kovačević-Filipović M. N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells. in Archives of Oral Biology. 2016;70:32-38.
doi:10.1016/j.archoralbio.2016.06.002 .

Debeljak-Martačić, Jasmina, Borozan, Sunčica, Radovanović, Anita, Popadić, Dusan, Mojsilović, Slavko, Vučić, Vesna M., Todorović, Vera, Kovačević-Filipović, Milica, "N-Acetyl-L-cysteine enhances ex-vivo amplification of deciduous teeth dental pulp stem cells" in Archives of Oral Biology, 70 (2016):32-38,
https://doi.org/10.1016/j.archoralbio.2016.06.002 . .

TY  - JOUR
AU  - Francuski, Jelena
AU  - Debeljak-Martačić, Jasmina
AU  - Radovanović, Anita
AU  - Andrić, Nenad
AU  - Sourice-Petit, Sophie
AU  - Guicheux, Jerome
AU  - Mojsilović, Slavko
AU  - Kovačević-Filipović, Milica
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/665
AB  - The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets' slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Proliferation and differentiation potential of canine synovial fluid cells
EP  - 78
IS  - 1
SP  - 66
VL  - 65
DO  - 10.1515/acve-2015-0005
ER  - 

@article{
author = "Francuski, Jelena and Debeljak-Martačić, Jasmina and Radovanović, Anita and Andrić, Nenad and Sourice-Petit, Sophie and Guicheux, Jerome and Mojsilović, Slavko and Kovačević-Filipović, Milica",
year = "2015",
abstract = "The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets' slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Proliferation and differentiation potential of canine synovial fluid cells",
pages = "78-66",
number = "1",
volume = "65",
doi = "10.1515/acve-2015-0005"
}

Francuski, J., Debeljak-Martačić, J., Radovanović, A., Andrić, N., Sourice-Petit, S., Guicheux, J., Mojsilović, S.,& Kovačević-Filipović, M.. (2015). Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 66-78.
https://doi.org/10.1515/acve-2015-0005

Francuski J, Debeljak-Martačić J, Radovanović A, Andrić N, Sourice-Petit S, Guicheux J, Mojsilović S, Kovačević-Filipović M. Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd. 2015;65(1):66-78.
doi:10.1515/acve-2015-0005 .

Francuski, Jelena, Debeljak-Martačić, Jasmina, Radovanović, Anita, Andrić, Nenad, Sourice-Petit, Sophie, Guicheux, Jerome, Mojsilović, Slavko, Kovačević-Filipović, Milica, "Proliferation and differentiation potential of canine synovial fluid cells" in Acta Veterinaria-Beograd, 65, no. 1 (2015):66-78,
https://doi.org/10.1515/acve-2015-0005 . .

TY  - JOUR
AU  - Debeljak-Martačić, Jasmina
AU  - Francuski, Jelena
AU  - Lužajić, Tijana
AU  - Vuković, Nemanja
AU  - Mojsilović, Slavko
AU  - Drndarević, Neda
AU  - Petakov, Marijana
AU  - Glibetić, Marija
AU  - Marković, Danica
AU  - Radovanović, Anita
AU  - Todorović, Vera
AU  - Kovačević-Filipović, Milica
PY  - 2014
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/528
AB  - Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix characteristics. Results. According to immunophenotype analyses all undifferentiated cells were positive for the mesenchymal stem cell markers: CD29 and CD73. Some cells expressed CD146 and CD106. The hematopoietic cell marker, CD34, was not detected. In passage one, incidence of CFU-F was 4.7 ± 0.5/100. Population doubling time did not change significantly during cell subcultivation and was in average 25 h. After induction of differentiation, the multicolony derived cell population had a tri-lineage differentiation potential, since mineralized matrix, cartilage-like tissue and adipocytes were successfully formed after three weeks of incubation. Conclusion. Altogether, these data suggest that remnants of deciduous teeth dental pulp contained cell populations with mesenchymal stem cell-like features, with a high proliferation and tri- lineage differentiation potential and that these cultures are suitable for further in vitro evaluation of cell based therapies.
AB  - Uvod/Cilj. Prošla dekada je bila posebno obeležena naporima na polju korišćenja ex vivo razvijenih i usmeravanih mezenhimskih matičnih ćelija (MSCs), kao sredstva za različite tipove regenerativne terapije. Cilj ove studije bio je da se utvrdi imunofenotip i potencijal za proliferaciju i diferencijaciju ćelija izolovanih iz zubne pulpe mlečnih zuba dece eksfoliranih u periodu kada je koren zuba bio u poslednjoj fazi resorpcije. Metode. Primarna adherentna populacija ćelija poreklom od pet donora dobijena je metodom eksplanta. Prisustvo progenitorskih ćelija koje obrazuju kolonije fibroblasta (CFU-F) pokazano je u prvoj pasaži. Do treće pasaže ćelije su ekspandirane, a potom korišćene za analiziranje. Imunofenotip je određen korišćenjem protočne citometrije. Proliferativni potencijal i vreme udvajanja ćelija (PDT) u kulturi je definisano na osnovu apsolutnog broja ćelija na početku i na kraju svake pasaže. Posle tronedeljne kultivacije ćelija u komercijalnim medijumima za stimulaciju osteogeneze, hondrogeneze i adipogeneze, citološkim i histološkim metodama je određena morfologija ćelija i karakteristike vanćelijskog matriksa. Rezultati. Antigeni koji karakterišu mezenhimske matične ćelije CD29 i CD73 su bili eksprimirani na svim nediferenciranim ćelijama, dok su antigeni CD146 i CD106 bili eksprimirani na ograničenom broju ćelija. Antigen CD34 (karakterističan za ćelije hematopoetske loze) nije bio eksprimiran. Incidencija CFU-F bila je 4,7 ± 0,5/100 ćelija. PDT se nije menjao tokom osam pasaža i u proseku je iznosio 25 h. Posle tronedeljne stimulacije diferencijacije u kulturama sa adipogenim medijumom došlo je do stvaranja ćelija sa masnim kapljicama, a u kulturama sa osteogenim medijumom došlo je do formiranja vanćelijskog matriksa sa deponovanim kalcijumovim solima. U kulturama sa hondrogenim medijumom došlo je do stvaranja tkiva sličnog hrskavici i vanćelijskog matriksa sa glikozaminoglikanima i kolagenom II. Zaključak. Zubna pulpa mlečnih zuba dece sadrži ćelijsku populaciju koja odgovara mezenhimskim matičnim ćelijama prema svojim karakteristikama, ima visok proliferativni potencijal i potencijal da se diferencira u tri ćelijske linije što je čini pogodnom za dalje in vitro analize i evaluaciju ćelijske terapije.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Characterization of deciduous teeth stem cells isolated from crown dental pulp
T1  - Karakterizacija matičnih ćelija izolovanih iz zubne pulpe mlečnih zuba dece
EP  - 741
IS  - 8
SP  - 735
VL  - 71
DO  - 10.2298/VSP1408735D
ER  - 

@article{
author = "Debeljak-Martačić, Jasmina and Francuski, Jelena and Lužajić, Tijana and Vuković, Nemanja and Mojsilović, Slavko and Drndarević, Neda and Petakov, Marijana and Glibetić, Marija and Marković, Danica and Radovanović, Anita and Todorović, Vera and Kovačević-Filipović, Milica",
year = "2014",
abstract = "Background/Aim. The last decade has been profoundly marked by persistent attempts to use ex vivo expanded and manipulated mesenchymal stem cells (MSCs), as a tool in different types of regenerative therapy. In the present study we described immunophenotype and the proliferative and differentiation potential of cells isolated from pulp remnants of exfoliated deciduous teeth in the final phase of root resorption. Methods. The initial adherent cell population from five donors was obtained by the outgrowth method. Colony forming unit-fibroblast (CFU-F) assay was performed in passage one. Cell expansion was performed until passage three and all tests were done until passage eight. Cells were labeled for early mesenchymal stem cells markers and analysis have been done using flow cytometry. The proliferative potential was assessed by cell counting in defined time points and population doubling time was calculated. Commercial media were used to induce osteoblastic, chondrogenic and adipogenic differentiation. Cytology and histology methods were used for analysis of differentiated cell morphology and extracellular matrix characteristics. Results. According to immunophenotype analyses all undifferentiated cells were positive for the mesenchymal stem cell markers: CD29 and CD73. Some cells expressed CD146 and CD106. The hematopoietic cell marker, CD34, was not detected. In passage one, incidence of CFU-F was 4.7 ± 0.5/100. Population doubling time did not change significantly during cell subcultivation and was in average 25 h. After induction of differentiation, the multicolony derived cell population had a tri-lineage differentiation potential, since mineralized matrix, cartilage-like tissue and adipocytes were successfully formed after three weeks of incubation. Conclusion. Altogether, these data suggest that remnants of deciduous teeth dental pulp contained cell populations with mesenchymal stem cell-like features, with a high proliferation and tri- lineage differentiation potential and that these cultures are suitable for further in vitro evaluation of cell based therapies., Uvod/Cilj. Prošla dekada je bila posebno obeležena naporima na polju korišćenja ex vivo razvijenih i usmeravanih mezenhimskih matičnih ćelija (MSCs), kao sredstva za različite tipove regenerativne terapije. Cilj ove studije bio je da se utvrdi imunofenotip i potencijal za proliferaciju i diferencijaciju ćelija izolovanih iz zubne pulpe mlečnih zuba dece eksfoliranih u periodu kada je koren zuba bio u poslednjoj fazi resorpcije. Metode. Primarna adherentna populacija ćelija poreklom od pet donora dobijena je metodom eksplanta. Prisustvo progenitorskih ćelija koje obrazuju kolonije fibroblasta (CFU-F) pokazano je u prvoj pasaži. Do treće pasaže ćelije su ekspandirane, a potom korišćene za analiziranje. Imunofenotip je određen korišćenjem protočne citometrije. Proliferativni potencijal i vreme udvajanja ćelija (PDT) u kulturi je definisano na osnovu apsolutnog broja ćelija na početku i na kraju svake pasaže. Posle tronedeljne kultivacije ćelija u komercijalnim medijumima za stimulaciju osteogeneze, hondrogeneze i adipogeneze, citološkim i histološkim metodama je određena morfologija ćelija i karakteristike vanćelijskog matriksa. Rezultati. Antigeni koji karakterišu mezenhimske matične ćelije CD29 i CD73 su bili eksprimirani na svim nediferenciranim ćelijama, dok su antigeni CD146 i CD106 bili eksprimirani na ograničenom broju ćelija. Antigen CD34 (karakterističan za ćelije hematopoetske loze) nije bio eksprimiran. Incidencija CFU-F bila je 4,7 ± 0,5/100 ćelija. PDT se nije menjao tokom osam pasaža i u proseku je iznosio 25 h. Posle tronedeljne stimulacije diferencijacije u kulturama sa adipogenim medijumom došlo je do stvaranja ćelija sa masnim kapljicama, a u kulturama sa osteogenim medijumom došlo je do formiranja vanćelijskog matriksa sa deponovanim kalcijumovim solima. U kulturama sa hondrogenim medijumom došlo je do stvaranja tkiva sličnog hrskavici i vanćelijskog matriksa sa glikozaminoglikanima i kolagenom II. Zaključak. Zubna pulpa mlečnih zuba dece sadrži ćelijsku populaciju koja odgovara mezenhimskim matičnim ćelijama prema svojim karakteristikama, ima visok proliferativni potencijal i potencijal da se diferencira u tri ćelijske linije što je čini pogodnom za dalje in vitro analize i evaluaciju ćelijske terapije.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Characterization of deciduous teeth stem cells isolated from crown dental pulp, Karakterizacija matičnih ćelija izolovanih iz zubne pulpe mlečnih zuba dece",
pages = "741-735",
number = "8",
volume = "71",
doi = "10.2298/VSP1408735D"
}

Debeljak-Martačić, J., Francuski, J., Lužajić, T., Vuković, N., Mojsilović, S., Drndarević, N., Petakov, M., Glibetić, M., Marković, D., Radovanović, A., Todorović, V.,& Kovačević-Filipović, M.. (2014). Characterization of deciduous teeth stem cells isolated from crown dental pulp. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 71(8), 735-741.
https://doi.org/10.2298/VSP1408735D

Debeljak-Martačić J, Francuski J, Lužajić T, Vuković N, Mojsilović S, Drndarević N, Petakov M, Glibetić M, Marković D, Radovanović A, Todorović V, Kovačević-Filipović M. Characterization of deciduous teeth stem cells isolated from crown dental pulp. in Vojnosanitetski pregled. 2014;71(8):735-741.
doi:10.2298/VSP1408735D .

Debeljak-Martačić, Jasmina, Francuski, Jelena, Lužajić, Tijana, Vuković, Nemanja, Mojsilović, Slavko, Drndarević, Neda, Petakov, Marijana, Glibetić, Marija, Marković, Danica, Radovanović, Anita, Todorović, Vera, Kovačević-Filipović, Milica, "Characterization of deciduous teeth stem cells isolated from crown dental pulp" in Vojnosanitetski pregled, 71, no. 8 (2014):735-741,
https://doi.org/10.2298/VSP1408735D . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Ilić, Vesna
AU  - Vujcić, Zoran
AU  - Dojnov, Biljana
AU  - Stevanov-Pavlović, Marija
AU  - Mijacević, Zora
AU  - Božić, Tatjana
PY  - 2012
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/447
AB  - Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.
PB  - Elsevier, Amsterdam
T2  - Veterinary Immunology & Immunopathology
T1  - Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis
EP  - 128
IS  - 1-2
SP  - 120
VL  - 145
DO  - 10.1016/j.vetimm.2011.10.015
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Ilić, Vesna and Vujcić, Zoran and Dojnov, Biljana and Stevanov-Pavlović, Marija and Mijacević, Zora and Božić, Tatjana",
year = "2012",
abstract = "Serum amyloid A proteins (SAA) are very sensitive acute phase proteins, displaying multiple isoforms in plasma and different body fluids. They are currently under investigation as biomarkers of diseases. The aim of the present study was to compare the concentration and isoform expression of SAA in serum and milk of cows with bacteriologically negative milk (control group) and naturally occurring Staphylococcus aureus (S. aureus) subclinical mastitis (subclinical mastitis group). Somatic cell count (SCC) and bacteriological analyses were performed to establish the control and subclinical mastitis group. SAA concentration was evaluated using a commercial ELISA kit, while expression of different isoforms (serum A-SAA and milk M-SAA3 isoforms) was visualized by denaturing isoelectrical focusing and immunoblotting. The SAA concentrations in sera and milk of cows in the subclinical mastitis group were three and 100 times higher than in those from the control group of cows, respectively. Cows in the subclinical mastitis group had more acidic SAA isoforms in serum with the most prominent one at pI 5.5. This isoform was not detected in sera from the control group. Milk samples in the subclinical mastitis group contained abundant highly alkaline M-SAA3 isoforms and most of the serum isoforms, except for that at pI 5.5. In the subclinical mastitis group SAA isoforms with equivalent pI as serum isoforms accounted for 20% of the total SAA concentration in milk. There were significant differences in the concentrations and isoform patterns of SAA in serum and milk between the control and subclinical mastitis groups of cows. Also, we demonstrated that serum SAA isoforms were not transferred to milk proportion to their plasma content.",
publisher = "Elsevier, Amsterdam",
journal = "Veterinary Immunology & Immunopathology",
title = "Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis",
pages = "128-120",
number = "1-2",
volume = "145",
doi = "10.1016/j.vetimm.2011.10.015"
}

Kovačević-Filipović, M., Ilić, V., Vujcić, Z., Dojnov, B., Stevanov-Pavlović, M., Mijacević, Z.,& Božić, T.. (2012). Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. in Veterinary Immunology & Immunopathology
Elsevier, Amsterdam., 145(1-2), 120-128.
https://doi.org/10.1016/j.vetimm.2011.10.015

Kovačević-Filipović M, Ilić V, Vujcić Z, Dojnov B, Stevanov-Pavlović M, Mijacević Z, Božić T. Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis. in Veterinary Immunology & Immunopathology. 2012;145(1-2):120-128.
doi:10.1016/j.vetimm.2011.10.015 .

Kovačević-Filipović, Milica, Ilić, Vesna, Vujcić, Zoran, Dojnov, Biljana, Stevanov-Pavlović, Marija, Mijacević, Zora, Božić, Tatjana, "Serum amyloid A isoforms in serum and milk from cows with Staphylococcus aureus subclinical mastitis" in Veterinary Immunology & Immunopathology, 145, no. 1-2 (2012):120-128,
https://doi.org/10.1016/j.vetimm.2011.10.015 . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Stevanović, Jelka
AU  - Stevanov-Pavlović, Marija
AU  - Debeljak-Martačić, Jasmina
AU  - Knežević, Milijana
AU  - Mijačević, Zora
AU  - Božić, Tatjana
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/307
AB  - Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs) with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30) were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group), second group were cows with one quarter infected with Staph. aureus (SaQ1) and third group were cows with two quarters infected (SaQ2). Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA) and somatic cell count (SCC). Serum samples where analyzed for SAA, haptoglobin (Hp), ceruloplasmin (Cp) and albumin concentration. Also, complete blood count (CBC) was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved.
AB  - Lokalna i sistemska zapaljenjska reakcija predstavljaju kompleksne procese koji uključuju sintezu proteina akutne faze sa mnogobrojnim funkcijama u regulaciji samog zapaljenskog procesa. Cilj ovog rada je bio da se definiše lokalni i sistemski odgovor proteina akutne faze, koji je podstaknut prirodnom subkliničkom infekcijom mlečne žlezde sa Staph. aureus kod mlečnih krava, ali sa različitim brojem inficiranih četvrti. Mlečne krave (n=30) u sredini laktacionog perioda su podeljene u tri grupe. U prvoj grupi su bile krave sa bakteriološki negativnim uzorcima mleka (BN grupa), u drugoj krave sa jednom četvrti vimena inficiranom sa Staph. aureus (SaQ1), dok su u trećoj grupi bile krave sa dve četvrti vimena inficiranim sa Staph. aureus (SaQ2). Uzorke mleka smo analizirali na sledeće pokazatelje zapaljenja: serum amiloid A (SAA) i broj somatskih ćelija (BSĆ). U uzorcima krvnog seruma su ispitivani SAA, haptoglobin (Hp), ceruloplazmin (Cp) i koncentracija albumina. Takođe je urađena kompletna krvna slika. BSĆ i SAA su bili najniži u mleku iz BN grupe i najviši u SaQ2 grupi. U uzorcima krvnog seruma SAA, Hp, Cp i koncentracija albumina bili su značajno viši samo u SaQ2 grupi u poređenju sa BN grupom. Broj leukocita kao i koncentracija haptoglobina su bili u fiziološkim granicama kod sve tri grupe krava. Ovi rezultati ukazuju da krave koje imaju subkliničku infekciju sa Staph. aureus u dve četvrti vimena imaju i jače izraženi sistemski odgovor proteina akutne faze nego krave kojima je inficirana samo jedna četvrt vimena čime je pokazano da opseg povrede tkiva ima uticaj na koncentraciju proteina akutne faze u serumu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Acute phase protein response in cows with staphylococcus aureus subclinical mastitis
T1  - Reakcija proteina akutne faze kod krava sa subkliničkim mastitisom izazvanim staphilococcus aureus-om
EP  - 216
IS  - 2-3
SP  - 205
VL  - 60
DO  - 10.2298/AVB1003205K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Stevanović, Jelka and Stevanov-Pavlović, Marija and Debeljak-Martačić, Jasmina and Knežević, Milijana and Mijačević, Zora and Božić, Tatjana",
year = "2010",
abstract = "Inflammation at a local and systemic level is a complex process that involves the synthesis of acute phase proteins (APPs) with multiple functions in the regulation of the inflammatory process itself. The aim of this work was to define local and systemic APPs response induced by natural Staph. aureus subclinical infection of the mammary gland in dairy cows with a different number of quarters involved. Midlactation dairy cows (n=30) were devided into three groups. First group were cows with bacteriologically negative milk samples (BN group), second group were cows with one quarter infected with Staph. aureus (SaQ1) and third group were cows with two quarters infected (SaQ2). Milk samples were analyzed for inflammation indicators: serum amyloid A (SAA) and somatic cell count (SCC). Serum samples where analyzed for SAA, haptoglobin (Hp), ceruloplasmin (Cp) and albumin concentration. Also, complete blood count (CBC) was done. SCC and SAA increased in quarter milk samples, being lowest in the BN group and highest in the SaQ2 group. In serum samples, SAA, Hp, Cp and albumin concentrations were significantly higher only in the SaQ2 group comparing with BN group. The leukocyte number, as well as hemoglobin concentration were in the physiological range in all three groups of cows. These results confirm that the magnitude of tissue injury has an impact on APPs concentration. They also demonstrate that cows having Staph. aureus sublinical infections of two mammary quarters have a more pronounced systemic APP response than cows with only one quarter involved., Lokalna i sistemska zapaljenjska reakcija predstavljaju kompleksne procese koji uključuju sintezu proteina akutne faze sa mnogobrojnim funkcijama u regulaciji samog zapaljenskog procesa. Cilj ovog rada je bio da se definiše lokalni i sistemski odgovor proteina akutne faze, koji je podstaknut prirodnom subkliničkom infekcijom mlečne žlezde sa Staph. aureus kod mlečnih krava, ali sa različitim brojem inficiranih četvrti. Mlečne krave (n=30) u sredini laktacionog perioda su podeljene u tri grupe. U prvoj grupi su bile krave sa bakteriološki negativnim uzorcima mleka (BN grupa), u drugoj krave sa jednom četvrti vimena inficiranom sa Staph. aureus (SaQ1), dok su u trećoj grupi bile krave sa dve četvrti vimena inficiranim sa Staph. aureus (SaQ2). Uzorke mleka smo analizirali na sledeće pokazatelje zapaljenja: serum amiloid A (SAA) i broj somatskih ćelija (BSĆ). U uzorcima krvnog seruma su ispitivani SAA, haptoglobin (Hp), ceruloplazmin (Cp) i koncentracija albumina. Takođe je urađena kompletna krvna slika. BSĆ i SAA su bili najniži u mleku iz BN grupe i najviši u SaQ2 grupi. U uzorcima krvnog seruma SAA, Hp, Cp i koncentracija albumina bili su značajno viši samo u SaQ2 grupi u poređenju sa BN grupom. Broj leukocita kao i koncentracija haptoglobina su bili u fiziološkim granicama kod sve tri grupe krava. Ovi rezultati ukazuju da krave koje imaju subkliničku infekciju sa Staph. aureus u dve četvrti vimena imaju i jače izraženi sistemski odgovor proteina akutne faze nego krave kojima je inficirana samo jedna četvrt vimena čime je pokazano da opseg povrede tkiva ima uticaj na koncentraciju proteina akutne faze u serumu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Acute phase protein response in cows with staphylococcus aureus subclinical mastitis, Reakcija proteina akutne faze kod krava sa subkliničkim mastitisom izazvanim staphilococcus aureus-om",
pages = "216-205",
number = "2-3",
volume = "60",
doi = "10.2298/AVB1003205K"
}

Kovačević-Filipović, M., Stevanović, J., Stevanov-Pavlović, M., Debeljak-Martačić, J., Knežević, M., Mijačević, Z.,& Božić, T.. (2010). Acute phase protein response in cows with staphylococcus aureus subclinical mastitis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 205-216.
https://doi.org/10.2298/AVB1003205K

Kovačević-Filipović M, Stevanović J, Stevanov-Pavlović M, Debeljak-Martačić J, Knežević M, Mijačević Z, Božić T. Acute phase protein response in cows with staphylococcus aureus subclinical mastitis. in Acta Veterinaria-Beograd. 2010;60(2-3):205-216.
doi:10.2298/AVB1003205K .

Kovačević-Filipović, Milica, Stevanović, Jelka, Stevanov-Pavlović, Marija, Debeljak-Martačić, Jasmina, Knežević, Milijana, Mijačević, Zora, Božić, Tatjana, "Acute phase protein response in cows with staphylococcus aureus subclinical mastitis" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):205-216,
https://doi.org/10.2298/AVB1003205K . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Okić, Ivana
AU  - Petrićević, Tanja
AU  - Mojsilović, S.
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Milenković, Pavle B.
AU  - Petakov, Marijana
AU  - Radovanović, Anita
AU  - Božić, Tatjana
AU  - Ivanović, Z.
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/261
AB  - Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia.
AB  - Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Cultivation of hamster bone marrow haematopoietic stem and progenitor cells
T1  - Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka
EP  - 14
IS  - 1
SP  - 3
VL  - 60
DO  - 10.2298/AVB1001003K
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Okić, Ivana and Petrićević, Tanja and Mojsilović, S. and Krstić, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Milenković, Pavle B. and Petakov, Marijana and Radovanović, Anita and Božić, Tatjana and Ivanović, Z.",
year = "2010",
abstract = "Hamster, a hibernating animal, is an important experimental model in research on the influence of hypothermia on different physiological processes. A simple procedure for cultivation and identification of hamster hematopoetic stem cells (HSC) and hematopoetic progenitor cells (HPC) is a premise for a successful investigation upon hypothermia effects on hematopoiesis. The aim of this work was to evaluate the utilization of commercially available methylcellulose media (MC) and recombinant mouse and human cytokines for hamster HSC and HPC assays, in order to enable further studies on these cells. Hamster bone marrow mononuclear cells (BMMNC) were plated in MC containing cytokines that support mouse or human HPC growth. Also, BMMNC were resuspended in cytokine supplemented liquid media and incubated for 5 weeks with a four day monitoring of viable cell number. We demonstrated that hamster hematopoietic progenitor cells committed for erythroid lineage and myeloid lineage successfully formed recognizable colonies in both mouse and human MC, while multipotent progenitor cells formed colonies only in mouse MC. We also defined conditions for the evaluation of hamster HSC activity in liquid cultures, based on continuous 5 weeks HSC proliferation. The obtained results verify the utilization of mouse specific MC for further research on hamster HPC biology during hypothermia., Fiziološka hibernacija u koju hrčci ulaze prilikom izlaganja niskim temperaturama, čini ove životinje zanimljivim eksperimentalnim modelom za ispitivanje hematopoeze u uslovima hipotermije. Preduslov za ovo ispitivanje je postojanje jednostavne metode za kultivaciju i identifikaciju hematopoetskih ćelija hrčka. Cilj ovog rada je bio da se ispita mogućnost kultivacije progenitorskih ćelija hematopoeze hrčka u kompletnoj metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, kao i da se odrede optimalni uslovi za kultivaciju matičnih ćelija hematopoeze hrčka u tečnoj kulturi. Mononuklearne ćelije kostne srži hrčka su posađene u metil celulozu i u tečnu kulturu. Oba medijuma su sadržala kombinacije rekombinantnih mišijih i/ili humanih citokina. Kolonije progenitorskih ćelija opredeljenih za mijelopoezu i opredeljenih za eritropoezu su se formirale u metil celulozi dizajniranoj za kultivaciju mišijih i humanih hematopoetskih ćelija, dok su se primitivnije kolonije sastavljene od oba tipa ćelija (mijeloidna i eritrocitna loza) formirale samo u metil celulozi dizajniranoj za kultivaciju mišijih hematopoetskih ćelija. Osim toga, populacija matičnih ćelija hematopoeze hrčka je proliferisala u tečnim kulturama tokom 5 nedelja bez znakova opadanja proliferativnog potencijala. Ova istraživanja pokazuju da se primenjene metode mogu uspešno koristiti za ispitivanje hematopoeze kod hrčka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells, Kultivacija matičnih i progenitorskih ćelija hematopoeze iz kostne srži hrčka",
pages = "14-3",
number = "1",
volume = "60",
doi = "10.2298/AVB1001003K"
}

Kovačević-Filipović, M., Okić, I., Petrićević, T., Mojsilović, S., Krstić, A., Jovčić, G., Bugarski, D., Milenković, P. B., Petakov, M., Radovanović, A., Božić, T.,& Ivanović, Z.. (2010). Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1), 3-14.
https://doi.org/10.2298/AVB1001003K

Kovačević-Filipović M, Okić I, Petrićević T, Mojsilović S, Krstić A, Jovčić G, Bugarski D, Milenković PB, Petakov M, Radovanović A, Božić T, Ivanović Z. Cultivation of hamster bone marrow haematopoietic stem and progenitor cells. in Acta Veterinaria-Beograd. 2010;60(1):3-14.
doi:10.2298/AVB1001003K .

Kovačević-Filipović, Milica, Okić, Ivana, Petrićević, Tanja, Mojsilović, S., Krstić, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Milenković, Pavle B., Petakov, Marijana, Radovanović, Anita, Božić, Tatjana, Ivanović, Z., "Cultivation of hamster bone marrow haematopoietic stem and progenitor cells" in Acta Veterinaria-Beograd, 60, no. 1 (2010):3-14,
https://doi.org/10.2298/AVB1001003K . .

TY  - JOUR
AU  - Jović, Slavoljub
AU  - Aleksić, Jelena
AU  - Krstić, Aleksandra
AU  - Stevanović, Jelka
AU  - Kovačević-Filipović, Milica
AU  - Borozan, Sunčica
AU  - Božić, Tatjana
AU  - Popović, Dragana
PY  - 2009
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/236
AB  - The paper presents the results of several health status parameters of sheep bred in the area exposed to depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. The blood samples of sheep were collected randomly in the region of Bujanovac, in 2004. Complete blood count was performed according to standard laboratory procedures. Concentration of red blood cells malondialdehyde (RBC MDA) and activity of erythrocyte superoxid dismutase (SOD) were determined spectrophotometrically, while the functional activity of leukocytes was investigated by NBT reduction and adhesion test. The results of complete red blood cells count indicated normocytic hypochromic anaemia. The total number of leukocytes and the differential leukocytes count were within the physiological range. Although the leukocytes adhesiveness was not changed in DU exposed animals, their increased NBT reduction revealed stimulated leukocytes' oxidative burst. This finding, together with significantly increased RBC MDA concentrations, as well as the activity of RBC antioxidant SOD, point to the existence of oxidative stress. Although the results indicated that animals were under oxidative stress, still there are no conclusive data that it was due to the exposure of DU that entered the environment during military operations.
AB  - U ovom radu su prikazani rezultati ispitivanja zdravstvenog stanja ovaca odgajanih u regionu koji je bio izložen delovanju municije sa osiromašenim uranijumom (DU) tokom bombardovanja Srbije i Crne Gore 1999. godine, od strane NATO pakta. Uzorci krvi su uzeti tokom 2004. godine od ovaca u regionu Bujanovca, metodom slučajnog izbora. Kompletan pregled krvi obavljen je standardnim laboratorijskim procedurama. Koncentracija malondialdehida u ertirocitima (RBC MDA) i aktivnost eritrocitne superoksid dismutaze (SOD) određene su spektrofotometrijski, dok je funkcionalna aktivnost leukocita ispitana NBT testom redukcije i adhezije. Rezultati kompletnog pregleda eritrocita su ukazali na postojanje normocitne hipohromne anemije. Ukupan broj leukocita i leukocitna formula su bili u fiziološkim okvirima. Kod životinja izloženih delovanju osiromašenog uranijuma nije bila izmenjena adhezivnost leukocita, ali je pojačana NBT redukcija ukazivala na to da je bio podstaknut njihov oksidativni prasak. Ovaj nalaz, uz značajan porast koncentracije RBC MDA i aktivnosti eritrocitnog antioksidativnog enzima SOD, ukazivali su na postojanje oksidativnog stresa. Rezultati dobijeni ovim ispitivanjima su upućivali na to da su životinje bile pod oksidativnim stresom, ali nemamo sigurne podatke da je on bio posledica izlaganja dejstvu osiromašenog uranijuma (DU) koji je dospeo u njihovu životnu sredinu tokom vojne akcije u tom regionu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition
T1  - Ispitivanje oksidativnog stresa i krvne slike ovaca odgajanih u regiji koja je bila izložena delovanju municije sa osiromašenim urnijumom (DU)
EP  - 488
IS  - 5-6
SP  - 481
VL  - 59
DO  - 10.2298/AVB0906481J
ER  - 

@article{
author = "Jović, Slavoljub and Aleksić, Jelena and Krstić, Aleksandra and Stevanović, Jelka and Kovačević-Filipović, Milica and Borozan, Sunčica and Božić, Tatjana and Popović, Dragana",
year = "2009",
abstract = "The paper presents the results of several health status parameters of sheep bred in the area exposed to depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. The blood samples of sheep were collected randomly in the region of Bujanovac, in 2004. Complete blood count was performed according to standard laboratory procedures. Concentration of red blood cells malondialdehyde (RBC MDA) and activity of erythrocyte superoxid dismutase (SOD) were determined spectrophotometrically, while the functional activity of leukocytes was investigated by NBT reduction and adhesion test. The results of complete red blood cells count indicated normocytic hypochromic anaemia. The total number of leukocytes and the differential leukocytes count were within the physiological range. Although the leukocytes adhesiveness was not changed in DU exposed animals, their increased NBT reduction revealed stimulated leukocytes' oxidative burst. This finding, together with significantly increased RBC MDA concentrations, as well as the activity of RBC antioxidant SOD, point to the existence of oxidative stress. Although the results indicated that animals were under oxidative stress, still there are no conclusive data that it was due to the exposure of DU that entered the environment during military operations., U ovom radu su prikazani rezultati ispitivanja zdravstvenog stanja ovaca odgajanih u regionu koji je bio izložen delovanju municije sa osiromašenim uranijumom (DU) tokom bombardovanja Srbije i Crne Gore 1999. godine, od strane NATO pakta. Uzorci krvi su uzeti tokom 2004. godine od ovaca u regionu Bujanovca, metodom slučajnog izbora. Kompletan pregled krvi obavljen je standardnim laboratorijskim procedurama. Koncentracija malondialdehida u ertirocitima (RBC MDA) i aktivnost eritrocitne superoksid dismutaze (SOD) određene su spektrofotometrijski, dok je funkcionalna aktivnost leukocita ispitana NBT testom redukcije i adhezije. Rezultati kompletnog pregleda eritrocita su ukazali na postojanje normocitne hipohromne anemije. Ukupan broj leukocita i leukocitna formula su bili u fiziološkim okvirima. Kod životinja izloženih delovanju osiromašenog uranijuma nije bila izmenjena adhezivnost leukocita, ali je pojačana NBT redukcija ukazivala na to da je bio podstaknut njihov oksidativni prasak. Ovaj nalaz, uz značajan porast koncentracije RBC MDA i aktivnosti eritrocitnog antioksidativnog enzima SOD, ukazivali su na postojanje oksidativnog stresa. Rezultati dobijeni ovim ispitivanjima su upućivali na to da su životinje bile pod oksidativnim stresom, ali nemamo sigurne podatke da je on bio posledica izlaganja dejstvu osiromašenog uranijuma (DU) koji je dospeo u njihovu životnu sredinu tokom vojne akcije u tom regionu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition, Ispitivanje oksidativnog stresa i krvne slike ovaca odgajanih u regiji koja je bila izložena delovanju municije sa osiromašenim urnijumom (DU)",
pages = "488-481",
number = "5-6",
volume = "59",
doi = "10.2298/AVB0906481J"
}

Jović, S., Aleksić, J., Krstić, A., Stevanović, J., Kovačević-Filipović, M., Borozan, S., Božić, T.,& Popović, D.. (2009). A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 481-488.
https://doi.org/10.2298/AVB0906481J

Jović S, Aleksić J, Krstić A, Stevanović J, Kovačević-Filipović M, Borozan S, Božić T, Popović D. A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition. in Acta Veterinaria-Beograd. 2009;59(5-6):481-488.
doi:10.2298/AVB0906481J .

Jović, Slavoljub, Aleksić, Jelena, Krstić, Aleksandra, Stevanović, Jelka, Kovačević-Filipović, Milica, Borozan, Sunčica, Božić, Tatjana, Popović, Dragana, "A study on oxidative stress and complete blood count of sheep bred in the area exposed to depleted uranium (DU) ammunition" in Acta Veterinaria-Beograd, 59, no. 5-6 (2009):481-488,
https://doi.org/10.2298/AVB0906481J . .

TY  - JOUR
AU  - Kovačević-Filipović, Milica
AU  - Petakov, Marijana
AU  - Hermitte, Francis
AU  - Debeissat, Christelle
AU  - Krstić, Aleksandra
AU  - Jovčić, Gordana
AU  - Biligarski, Dijana
AU  - Lafarge, Xavier
AU  - Milenković, Pavle B.
AU  - Praloran, Vincent
AU  - Ivanović, Zoran
PY  - 2007
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/171
AB  - Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.
PB  - Wiley, Hoboken
T2  - Journal of Cellular Physiology
T1  - Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)
EP  - 75
IS  - 1
SP  - 68
VL  - 212
DO  - 10.1002/jcp.21003
ER  - 

@article{
author = "Kovačević-Filipović, Milica and Petakov, Marijana and Hermitte, Francis and Debeissat, Christelle and Krstić, Aleksandra and Jovčić, Gordana and Biligarski, Dijana and Lafarge, Xavier and Milenković, Pavle B. and Praloran, Vincent and Ivanović, Zoran",
year = "2007",
abstract = "Low O-2 concentration (1%) favors the self-renewal of hematopoietic stem cells and inhibits committed progenitors (CFC). Since IL-6 influences both stem cells and committed progenitors at 20% O-2, we studied its effects in cultures at 1% O-2. The pre-CFC activity in Lin- population of mouse bone marrow was analyzed following 10 days of serum-free culture in medium (LCI) supplemented with IL-3 with and without IL-6, at 20 and 1% O-2 and phenotypic differentiation and proliferative history monitored. The IL-6 receptor expression and initiation of VEGF-A synthesis were also investigated. At 20% O-2, the effects of IL-6 on pre-CFC were negligible but effects on CFC were apparent; conversely, at 1% O-2, the IL-6 enhances activity of pre-CFC but not of CFC. Unlike at 20% O-2, at 1% O-2 a subpopulation of cells remained Lin- in spite of extensive proliferation. However, the absolute number of Lin- cells, did not correlate with pre-CFC activity. A relative increase in VEGF transcripts at 1% O-2 in presence of IL-3 alone was enhanced by the addition of IL-6. IL-6 enhanced pre-CFC activity at 1% O-2 and this was correlated to the induction of VEGF. These data reinforce the concept that physiologically low oxygenation of bone marrow is a regulator of stem cell maintenance. Since the 20% O-2 does not exist in tissues in vivo, further studies in vitro at lower O-2 concentrations should revise our knowledge relating to cytokine effects on stem and progenitor cells.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)",
pages = "75-68",
number = "1",
volume = "212",
doi = "10.1002/jcp.21003"
}

Kovačević-Filipović, M., Petakov, M., Hermitte, F., Debeissat, C., Krstić, A., Jovčić, G., Biligarski, D., Lafarge, X., Milenković, P. B., Praloran, V.,& Ivanović, Z.. (2007). Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology
Wiley, Hoboken., 212(1), 68-75.
https://doi.org/10.1002/jcp.21003

Kovačević-Filipović M, Petakov M, Hermitte F, Debeissat C, Krstić A, Jovčić G, Biligarski D, Lafarge X, Milenković PB, Praloran V, Ivanović Z. Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC). in Journal of Cellular Physiology. 2007;212(1):68-75.
doi:10.1002/jcp.21003 .

Kovačević-Filipović, Milica, Petakov, Marijana, Hermitte, Francis, Debeissat, Christelle, Krstić, Aleksandra, Jovčić, Gordana, Biligarski, Dijana, Lafarge, Xavier, Milenković, Pavle B., Praloran, Vincent, Ivanović, Zoran, "Interleukin-6 (IL-6) and low O-2 concentration (1%) synergize to improve the maintenance of hematopoietic stem cells (Pre-CFC)" in Journal of Cellular Physiology, 212, no. 1 (2007):68-75,
https://doi.org/10.1002/jcp.21003 . .

TY  - JOUR
AU  - Ilić, Vesna
AU  - Petakov, Marijana
AU  - Stojanović, Nevenka
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Grbović, Tatjana
AU  - Božić, Tatjana
AU  - Kovačević-Filipović, Milica
PY  - 2006
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/151
AB  - Serum iron concentration and iron saturation of transferrin (Trf) are measures of body iron stores after administration of iron supplements. In clinical and experimental research, the complex determination of Trf was replaced by the simple determination of total iron binding capacity (TIBC). The objective of this work was to define if TIBC could be an adequate measure for Trf in neonatal piglets after i.m. iron administration. Treated piglets received 150 mg of iron-dextran i.m. the first day of life, and were compared to the untreated control group. Prior to iron administration, as well as on days 2, 8 and 12 after iron administration, serum iron and TIBC concentration were analyzed by an automatized chemical analyzer and Trf was determined by densitometry of electrophoretic strips. Our results show that regardless of iron treatment, TIBC is not a measure of Trf concentration in neonatal piglets two days after birth. At day 8 of their life a high correlation coefficient of these two parameters was established in non-treated animals, while in iron-treated piglets the same correlation was established 12 days after iron treatment. Thus, we suggest that in neonatal piglets, TIBC could be used as a measure of Trf concentration only 12 days after i.m. iron treatment.
AB  - Određivanje statusa gvožđa u organizmu jedinke posle primene određenog preparata ovog mikroelementa moguće je utvrditi određivanjem njegove koncentracije u serumu i zasićenja transferina (Trf) gvožđem. U kliničkoj i eksperimentalnoj praksi složeno određivanje koncentracije Trf zamenjeno je jednostavnim određ ivanjem ukupnog serumskog kapaciteta za vezivanje gvožđa (TIBC). Cilj ovog rada je bio da se na modelu porasta serumskog Fe po i.m. aplikaciji Fe-dextrana novorođenoj prasadi, utvrdi odnos TIBC i Trf po aplikaciji ovog mikroelementa, kako bi se utvrdilo da li visoke doze gvožđa u serumu utiču na vrednost TIBC kao mere za određivanje koncentracije Trf. Vrednosti za serumsko Fe, TIBC i Trf poređene su između grupe životinja koja je odmah po rođenju dobila 150 mg Fe-dextrana i kontrolne grupe u kojoj životoinje nisu tretirane Fe-dextranom. Krv je uzorkovana pre aplikacije Fe-dextrana, drugog, osmog i dvanaestog dana po aplikaciji preparata gvožđa. Koncentracija gvožđa u serumu i TIBC su određivani standarnim kliničkim biohemijskim analizama, dok je koncenracija Trf određena denzitometrijom elektroforetskih traka. Dobijeni rezultati ukazuju da bez obzira na primenu preparata Fe, TIBC nije adekvatna mera za Trf kod novorođene prasadi u prva tri dana po rođenju. Osmog dana života prasadi, utvrđen je visoki stepen korelacije ova dva parametra kod životinja koje nisu bile tretirane, dok je kod tretiranih jedinki taj stepen korelacije postignut dvanaestog dana. Na osnovu izloženih rezultata se može zaključiti da se kod novorođene prasadi TIBC može koristiti kao mera za Trf tek 12 dana nakon i.m. tretmana Fe-dextranom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran
T1  - Odnos između totalnog kapaciteta za vezivanje gvožđa i koncentracije transferina kod novorođene prasadi tretirane gvožđe-dekstranom
EP  - 242
IS  - 2-3
SP  - 235
VL  - 56
DO  - 10.2298/AVB0603235I
ER  - 

@article{
author = "Ilić, Vesna and Petakov, Marijana and Stojanović, Nevenka and Jovčić, Gordana and Bugarski, Diana and Grbović, Tatjana and Božić, Tatjana and Kovačević-Filipović, Milica",
year = "2006",
abstract = "Serum iron concentration and iron saturation of transferrin (Trf) are measures of body iron stores after administration of iron supplements. In clinical and experimental research, the complex determination of Trf was replaced by the simple determination of total iron binding capacity (TIBC). The objective of this work was to define if TIBC could be an adequate measure for Trf in neonatal piglets after i.m. iron administration. Treated piglets received 150 mg of iron-dextran i.m. the first day of life, and were compared to the untreated control group. Prior to iron administration, as well as on days 2, 8 and 12 after iron administration, serum iron and TIBC concentration were analyzed by an automatized chemical analyzer and Trf was determined by densitometry of electrophoretic strips. Our results show that regardless of iron treatment, TIBC is not a measure of Trf concentration in neonatal piglets two days after birth. At day 8 of their life a high correlation coefficient of these two parameters was established in non-treated animals, while in iron-treated piglets the same correlation was established 12 days after iron treatment. Thus, we suggest that in neonatal piglets, TIBC could be used as a measure of Trf concentration only 12 days after i.m. iron treatment., Određivanje statusa gvožđa u organizmu jedinke posle primene određenog preparata ovog mikroelementa moguće je utvrditi određivanjem njegove koncentracije u serumu i zasićenja transferina (Trf) gvožđem. U kliničkoj i eksperimentalnoj praksi složeno određivanje koncentracije Trf zamenjeno je jednostavnim određ ivanjem ukupnog serumskog kapaciteta za vezivanje gvožđa (TIBC). Cilj ovog rada je bio da se na modelu porasta serumskog Fe po i.m. aplikaciji Fe-dextrana novorođenoj prasadi, utvrdi odnos TIBC i Trf po aplikaciji ovog mikroelementa, kako bi se utvrdilo da li visoke doze gvožđa u serumu utiču na vrednost TIBC kao mere za određivanje koncentracije Trf. Vrednosti za serumsko Fe, TIBC i Trf poređene su između grupe životinja koja je odmah po rođenju dobila 150 mg Fe-dextrana i kontrolne grupe u kojoj životoinje nisu tretirane Fe-dextranom. Krv je uzorkovana pre aplikacije Fe-dextrana, drugog, osmog i dvanaestog dana po aplikaciji preparata gvožđa. Koncentracija gvožđa u serumu i TIBC su određivani standarnim kliničkim biohemijskim analizama, dok je koncenracija Trf određena denzitometrijom elektroforetskih traka. Dobijeni rezultati ukazuju da bez obzira na primenu preparata Fe, TIBC nije adekvatna mera za Trf kod novorođene prasadi u prva tri dana po rođenju. Osmog dana života prasadi, utvrđen je visoki stepen korelacije ova dva parametra kod životinja koje nisu bile tretirane, dok je kod tretiranih jedinki taj stepen korelacije postignut dvanaestog dana. Na osnovu izloženih rezultata se može zaključiti da se kod novorođene prasadi TIBC može koristiti kao mera za Trf tek 12 dana nakon i.m. tretmana Fe-dextranom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran, Odnos između totalnog kapaciteta za vezivanje gvožđa i koncentracije transferina kod novorođene prasadi tretirane gvožđe-dekstranom",
pages = "242-235",
number = "2-3",
volume = "56",
doi = "10.2298/AVB0603235I"
}

Ilić, V., Petakov, M., Stojanović, N., Jovčić, G., Bugarski, D., Grbović, T., Božić, T.,& Kovačević-Filipović, M.. (2006). Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(2-3), 235-242.
https://doi.org/10.2298/AVB0603235I

Ilić V, Petakov M, Stojanović N, Jovčić G, Bugarski D, Grbović T, Božić T, Kovačević-Filipović M. Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran. in Acta Veterinaria-Beograd. 2006;56(2-3):235-242.
doi:10.2298/AVB0603235I .

Ilić, Vesna, Petakov, Marijana, Stojanović, Nevenka, Jovčić, Gordana, Bugarski, Diana, Grbović, Tatjana, Božić, Tatjana, Kovačević-Filipović, Milica, "Relationship between total iron binding capacity and transferrin concentration in neonatal piglets treated with iron-dextran" in Acta Veterinaria-Beograd, 56, no. 2-3 (2006):235-242,
https://doi.org/10.2298/AVB0603235I . .

TY  - JOUR
AU  - Kovačević, Milica
AU  - Božić, Tatjana
AU  - Jovčić, Gordana
AU  - Petakov, Marijana
AU  - Bugarski, Diana
AU  - Stanković, Jelena
AU  - Ivanović, Z.
PY  - 2001
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/69
AB  - The pig is widely used as a large animal model for biomedical research and could be an interesting experimental model for studies of the hematopoietic system and its response in physiological and pathological conditions. With the intention of using the pig as a large animal model in hematopoietic research, a clonal assay in methylcellulose was developed and standardized for detection of committed progenitors of the granulocyte-macrophage lineage from adult pig bone marrow and peripheral blood. Progenitor cells were stimulated to proliferate and differentiate in vitro by adding pig leukocyte conditioned medium (LCM) as a source of homologous growth factors. The number of CFU-GM (Colony Forming Unit -Granulocyte-Macrophage) directly depended on the concentration of LCM. The proliferative rate of CFU-GM progenitor cells was determined by the cytosine arabinoside suicide technique. The percentage of bone marrow and peripheral blood CFU-GM cells in S phase of the cell cycle was 34.7% and 22.2%, respectively. The data obtained regarding the number and characteristics of pig bone marrow and peripheral blood CFU-GM confirmed that the organization of the pig CFU-GM progenitor cell compartment is similar and comparable to that in miniature swine, other animal species and humans.
AB  - Svinja je životinja koja se koristi kao model u različitim biomedicinskim istraživanjima, a mogia bi biti i interesantan model u ispitivanju fiziologije i patolofizioloških promena hematopoetskog sistema. U cilju razvoja eksperimentalnog modela svinje u istraživanju hematopoeze, stanadardizovan je esej za odredivanje i karakterizaciju opredeljenih matičnih ćelija granulocitno-monocitne loze iz kostne srži i krvi odrasle svinje. Stimulacija proliferacije i diferencijacije ovih matičnih ćelija postignuta je dodavanjem medijuma kondicioniranog leukocitima (LCM - Leukocyte conditioned medium) bogatog faktorima rasta. Broj CFU-GM (Colony forming unit- granulocyte-macrophage) je direktno zavisio od koncentracije LCM-a. Procenat CFU-GM ćelija u S fazi ćelijskog cikiusa odredjivan je tehnikom 'suicida' korišćenjem citozin arabinozida (Ara-C) i iznosio je 34.7% za CFU-GM iz kostne srži i 22.2% za CFU-GM iz periferne krvi. Podaci dobijeni za broj i karakteristike CFU-GM iz kostne srži i periferne krvi potvrđuju da je ovaj odeljak matičnih ćelija kod odraslih svinja organizovan na isti način kao i kod minijaturnih svinja, drugih vrsta životinja i ljudi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells
T1  - Matične ćelije opredeljene za granulocitno-monocitnu lozu kostne srži i periferne krvi svinja
EP  - 26
IS  - 1
SP  - 15
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_169
ER  - 

@article{
author = "Kovačević, Milica and Božić, Tatjana and Jovčić, Gordana and Petakov, Marijana and Bugarski, Diana and Stanković, Jelena and Ivanović, Z.",
year = "2001",
abstract = "The pig is widely used as a large animal model for biomedical research and could be an interesting experimental model for studies of the hematopoietic system and its response in physiological and pathological conditions. With the intention of using the pig as a large animal model in hematopoietic research, a clonal assay in methylcellulose was developed and standardized for detection of committed progenitors of the granulocyte-macrophage lineage from adult pig bone marrow and peripheral blood. Progenitor cells were stimulated to proliferate and differentiate in vitro by adding pig leukocyte conditioned medium (LCM) as a source of homologous growth factors. The number of CFU-GM (Colony Forming Unit -Granulocyte-Macrophage) directly depended on the concentration of LCM. The proliferative rate of CFU-GM progenitor cells was determined by the cytosine arabinoside suicide technique. The percentage of bone marrow and peripheral blood CFU-GM cells in S phase of the cell cycle was 34.7% and 22.2%, respectively. The data obtained regarding the number and characteristics of pig bone marrow and peripheral blood CFU-GM confirmed that the organization of the pig CFU-GM progenitor cell compartment is similar and comparable to that in miniature swine, other animal species and humans., Svinja je životinja koja se koristi kao model u različitim biomedicinskim istraživanjima, a mogia bi biti i interesantan model u ispitivanju fiziologije i patolofizioloških promena hematopoetskog sistema. U cilju razvoja eksperimentalnog modela svinje u istraživanju hematopoeze, stanadardizovan je esej za odredivanje i karakterizaciju opredeljenih matičnih ćelija granulocitno-monocitne loze iz kostne srži i krvi odrasle svinje. Stimulacija proliferacije i diferencijacije ovih matičnih ćelija postignuta je dodavanjem medijuma kondicioniranog leukocitima (LCM - Leukocyte conditioned medium) bogatog faktorima rasta. Broj CFU-GM (Colony forming unit- granulocyte-macrophage) je direktno zavisio od koncentracije LCM-a. Procenat CFU-GM ćelija u S fazi ćelijskog cikiusa odredjivan je tehnikom 'suicida' korišćenjem citozin arabinozida (Ara-C) i iznosio je 34.7% za CFU-GM iz kostne srži i 22.2% za CFU-GM iz periferne krvi. Podaci dobijeni za broj i karakteristike CFU-GM iz kostne srži i periferne krvi potvrđuju da je ovaj odeljak matičnih ćelija kod odraslih svinja organizovan na isti način kao i kod minijaturnih svinja, drugih vrsta životinja i ljudi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells, Matične ćelije opredeljene za granulocitno-monocitnu lozu kostne srži i periferne krvi svinja",
pages = "26-15",
number = "1",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_169"
}

Kovačević, M., Božić, T., Jovčić, G., Petakov, M., Bugarski, D., Stanković, J.,& Ivanović, Z.. (2001). Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 51(1), 15-26.
https://hdl.handle.net/21.15107/rcub_veterinar_169

Kovačević M, Božić T, Jovčić G, Petakov M, Bugarski D, Stanković J, Ivanović Z. Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells. in Acta Veterinaria-Beograd. 2001;51(1):15-26.
https://hdl.handle.net/21.15107/rcub_veterinar_169 .

Kovačević, Milica, Božić, Tatjana, Jovčić, Gordana, Petakov, Marijana, Bugarski, Diana, Stanković, Jelena, Ivanović, Z., "Pig bone marrow and peripheral blood granulocyte-macrophage progenitor cells" in Acta Veterinaria-Beograd, 51, no. 1 (2001):15-26,
https://hdl.handle.net/21.15107/rcub_veterinar_169 .

TY  - JOUR
AU  - Kovačević, Milica
AU  - Božić, Tatjana
AU  - Pavlović, V.
AU  - Petakov, Marijana
AU  - Bugarski, Diana
AU  - Jovčić, Gordana
AU  - Ivanović, Z.
PY  - 2000
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/57
AB  - The proliferative rate of hemopoietic progenitor cells, i.e. the percentage of cells in the synthetic (S) phase of the cell cycle is a very sensitive parameter for detecting the steady-state misbalance arising in response to stimulatory or inhibitory molecules, or in postmyeloablative repopulation of hemopoiesis. This parameter is also predictive for the sensitivity of these cells to irradiation injury and specific cytotoxic drugs. In spite of the fact that miniature inbred and domestic pig breeds have been used as large animal models in hemopoietic research and in experimental bone marrow transplantation, data concerning the proliferative rate of porcine progenitor cells are still lacking. The aim of this study was to examine the steady-state proliferative rate of bone marrow and peripheral blood erythroid progenitor cells of adult pigs. The percentage of cells in S phase of the cell cycle of both immature, BFU-E (Burst Forming Unit-Erythroid) and mature, CFU-E (Colony Forming Unit-Erythmid) erythroid progenitor cells was determined by the suicide technique based on the proportion of these cells killed after in vitro treatment of the cells with cytosine arabinoside (Ara-C). The results revealed different relative numbers of immature and mature erythroid progenitor cells in S phase of the cell cycle in the bone marrow, namely, 33.3% for BFU-E and 55.1% for CFU-E. In peripheral blood the proliferative rate of BFU-E progenitors was 26.3%. The data obtained show that the proliferative rate of porcine erythroid progenitor cells is similar to the values determined for other mammalian species.
AB  - Proliferativna aktivnost opredeljenih matičnih ćelija hematopoeze, odnosno procenat ovih ćelija u S (sintetskoj) fazi ćelijskog ciklusa je veoma osetljiv parametar za određivanje poremećajafizioloških vrednosti koje nastaju dejstvom stimulatomih ili inhibitornih molekula, ili kod repopulacije hematopoeze posle mijeloablacije. Ovaj parametar je takođe vredan u ocenjivanju osetljivosti matičnih ćelija na radijacione povrede ili određene citotoksične lekove. Uprkos činjenici da su kao eksperimentalni modeli u hematopoetskim istraživanjima i u eksperimentalnoj transplantaciji kostne srži korišćeni kako visoko-srodni sojevi minijaturnih, tako i industrijske rase svinja, literaturni podaci o proliferativnoj aktivnosti opredeljenih matičnih ćelija još uvek nedostaju. Cilj ovog istraživanja je bio da se odrede proliferativne aktivnosti opredeljenih matičnih ćelija eritropoeze iz kostne srži i periferne krvi odraslih svinja u fiziološkim uslovima. Procenat opredeljenih matičnih ćelija za eritropoezu, BFU-E (Burst forming unit-erythroid) i CFU-E (Colony forming unit-erythroid) u S fazi ćelijskog ciklusa je određivan tehnikom 'suicida'. Metoda se zasniva na in vitro 'ubijanju' ćelija u S fazi ćelijskog ciklusa citozin arabinozidom (Ara-C). Rezultati su pokazali da je procenat ranih i zrelih opredeljenih matičnih ćelija za eritropoezu u S fazi ćelijskog ciklusa različit, i iznosio je 33,3% za BFU-E i 55,1 % za CFU-E ćelije. U perifernoj krvi proliferativna aktivnost BFU-E je iznosila 26,3%. Dobijeni rezultati pokazuju da je proliferativna aktivnost opredeljenih matičnih ćelija eritropoeze kod svinja slična vrednostima dobijenim kod drugih vrsta sisara.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle
T1  - Opredeljene matične ćelije eritropoeze kostne srži i periferne krvi svinja u S fazi ćelijskog ciklusa
EP  - 214
IS  - 4
SP  - 207
VL  - 50
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_146
ER  - 

@article{
author = "Kovačević, Milica and Božić, Tatjana and Pavlović, V. and Petakov, Marijana and Bugarski, Diana and Jovčić, Gordana and Ivanović, Z.",
year = "2000",
abstract = "The proliferative rate of hemopoietic progenitor cells, i.e. the percentage of cells in the synthetic (S) phase of the cell cycle is a very sensitive parameter for detecting the steady-state misbalance arising in response to stimulatory or inhibitory molecules, or in postmyeloablative repopulation of hemopoiesis. This parameter is also predictive for the sensitivity of these cells to irradiation injury and specific cytotoxic drugs. In spite of the fact that miniature inbred and domestic pig breeds have been used as large animal models in hemopoietic research and in experimental bone marrow transplantation, data concerning the proliferative rate of porcine progenitor cells are still lacking. The aim of this study was to examine the steady-state proliferative rate of bone marrow and peripheral blood erythroid progenitor cells of adult pigs. The percentage of cells in S phase of the cell cycle of both immature, BFU-E (Burst Forming Unit-Erythroid) and mature, CFU-E (Colony Forming Unit-Erythmid) erythroid progenitor cells was determined by the suicide technique based on the proportion of these cells killed after in vitro treatment of the cells with cytosine arabinoside (Ara-C). The results revealed different relative numbers of immature and mature erythroid progenitor cells in S phase of the cell cycle in the bone marrow, namely, 33.3% for BFU-E and 55.1% for CFU-E. In peripheral blood the proliferative rate of BFU-E progenitors was 26.3%. The data obtained show that the proliferative rate of porcine erythroid progenitor cells is similar to the values determined for other mammalian species., Proliferativna aktivnost opredeljenih matičnih ćelija hematopoeze, odnosno procenat ovih ćelija u S (sintetskoj) fazi ćelijskog ciklusa je veoma osetljiv parametar za određivanje poremećajafizioloških vrednosti koje nastaju dejstvom stimulatomih ili inhibitornih molekula, ili kod repopulacije hematopoeze posle mijeloablacije. Ovaj parametar je takođe vredan u ocenjivanju osetljivosti matičnih ćelija na radijacione povrede ili određene citotoksične lekove. Uprkos činjenici da su kao eksperimentalni modeli u hematopoetskim istraživanjima i u eksperimentalnoj transplantaciji kostne srži korišćeni kako visoko-srodni sojevi minijaturnih, tako i industrijske rase svinja, literaturni podaci o proliferativnoj aktivnosti opredeljenih matičnih ćelija još uvek nedostaju. Cilj ovog istraživanja je bio da se odrede proliferativne aktivnosti opredeljenih matičnih ćelija eritropoeze iz kostne srži i periferne krvi odraslih svinja u fiziološkim uslovima. Procenat opredeljenih matičnih ćelija za eritropoezu, BFU-E (Burst forming unit-erythroid) i CFU-E (Colony forming unit-erythroid) u S fazi ćelijskog ciklusa je određivan tehnikom 'suicida'. Metoda se zasniva na in vitro 'ubijanju' ćelija u S fazi ćelijskog ciklusa citozin arabinozidom (Ara-C). Rezultati su pokazali da je procenat ranih i zrelih opredeljenih matičnih ćelija za eritropoezu u S fazi ćelijskog ciklusa različit, i iznosio je 33,3% za BFU-E i 55,1 % za CFU-E ćelije. U perifernoj krvi proliferativna aktivnost BFU-E je iznosila 26,3%. Dobijeni rezultati pokazuju da je proliferativna aktivnost opredeljenih matičnih ćelija eritropoeze kod svinja slična vrednostima dobijenim kod drugih vrsta sisara.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle, Opredeljene matične ćelije eritropoeze kostne srži i periferne krvi svinja u S fazi ćelijskog ciklusa",
pages = "214-207",
number = "4",
volume = "50",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_146"
}

Kovačević, M., Božić, T., Pavlović, V., Petakov, M., Bugarski, D., Jovčić, G.,& Ivanović, Z.. (2000). Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 50(4), 207-214.
https://hdl.handle.net/21.15107/rcub_veterinar_146

Kovačević M, Božić T, Pavlović V, Petakov M, Bugarski D, Jovčić G, Ivanović Z. Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle. in Acta Veterinaria-Beograd. 2000;50(4):207-214.
https://hdl.handle.net/21.15107/rcub_veterinar_146 .

Kovačević, Milica, Božić, Tatjana, Pavlović, V., Petakov, Marijana, Bugarski, Diana, Jovčić, Gordana, Ivanović, Z., "Pig bone marrow and peripheral blood erythroid progenitor cells in S phase of the cell cycle" in Acta Veterinaria-Beograd, 50, no. 4 (2000):207-214,
https://hdl.handle.net/21.15107/rcub_veterinar_146 .