TY  - JOUR
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Bogosavljević, Nikola
AU  - Jurišić, Vladimir
AU  - Jauković, Aleksandra
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1460
AB  - Among multiple hemostasis components, platelets hyperactivity plays major roles in cancer progression by providing surface and internal components for intercellular crosstalk as well as by behaving like immune cells. Since platelets participate and regulate immunity in homeostatic and disease states, we assumed that revealing platelets profile might help in conceiving novel anti-cancer immune-based strategies. The goal of this review is to compile and discuss the most recent reports on the nature of cancer-associated platelets and their interference with immunotherapy. An increasing number of studies have emphasized active communication between cancer cells and platelets, with platelets promoting cancer cell survival, growth, and metastasis. The anti-cancer potential of platelet-directed therapy has been intensively investigated, and anti-platelet agents may prevent cancer progression and improve the survival of cancer patients. Platelets can (i) reduce antitumor activity; (ii) support immunoregulatory cells and factors generation; (iii) underpin metastasis and, (iv) interfere with immunotherapy by expressing ligands of immune checkpoint receptors. Mediators produced by tumor cell-induced platelet activation support vein thrombosis, constrain anti-tumor T- and natural killer cell response, while contributing to extravasation of tumor cells, metastatic potential, and neovascularization within the tumor. Recent studies showed that attenuation of immunothrombosis, modulation of platelets and their factors have a good perspective in immunotherapy optimization. Particularly, blockade of intra-tumoral platelet-associated programmed death-ligand 1 might promote anti-tumor T cell-induced cytotoxicity. Collectively, these findings suggest that platelets might represent the source of relevant cancer staging biomarkers, as well as promising targets and carriers in immunotherapeutic approaches for combating cancer.
PB  - Neoplasia Press, Inc.
T2  - Translational Oncology
T2  - Translational OncologyTranslational Oncology
T1  - Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development
SP  - 101871
VL  - 40
DO  - 10.1016/j.tranon.2023.101871
ER  - 

@article{
author = "Trivanović, Drenka and Mojsilović, Slavko and Bogosavljević, Nikola and Jurišić, Vladimir and Jauković, Aleksandra",
year = "2024",
abstract = "Among multiple hemostasis components, platelets hyperactivity plays major roles in cancer progression by providing surface and internal components for intercellular crosstalk as well as by behaving like immune cells. Since platelets participate and regulate immunity in homeostatic and disease states, we assumed that revealing platelets profile might help in conceiving novel anti-cancer immune-based strategies. The goal of this review is to compile and discuss the most recent reports on the nature of cancer-associated platelets and their interference with immunotherapy. An increasing number of studies have emphasized active communication between cancer cells and platelets, with platelets promoting cancer cell survival, growth, and metastasis. The anti-cancer potential of platelet-directed therapy has been intensively investigated, and anti-platelet agents may prevent cancer progression and improve the survival of cancer patients. Platelets can (i) reduce antitumor activity; (ii) support immunoregulatory cells and factors generation; (iii) underpin metastasis and, (iv) interfere with immunotherapy by expressing ligands of immune checkpoint receptors. Mediators produced by tumor cell-induced platelet activation support vein thrombosis, constrain anti-tumor T- and natural killer cell response, while contributing to extravasation of tumor cells, metastatic potential, and neovascularization within the tumor. Recent studies showed that attenuation of immunothrombosis, modulation of platelets and their factors have a good perspective in immunotherapy optimization. Particularly, blockade of intra-tumoral platelet-associated programmed death-ligand 1 might promote anti-tumor T cell-induced cytotoxicity. Collectively, these findings suggest that platelets might represent the source of relevant cancer staging biomarkers, as well as promising targets and carriers in immunotherapeutic approaches for combating cancer.",
publisher = "Neoplasia Press, Inc.",
journal = "Translational Oncology, Translational OncologyTranslational Oncology",
title = "Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development",
pages = "101871",
volume = "40",
doi = "10.1016/j.tranon.2023.101871"
}

Trivanović, D., Mojsilović, S., Bogosavljević, N., Jurišić, V.,& Jauković, A.. (2024). Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development. in Translational Oncology
Neoplasia Press, Inc.., 40, 101871.
https://doi.org/10.1016/j.tranon.2023.101871

Trivanović D, Mojsilović S, Bogosavljević N, Jurišić V, Jauković A. Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development. in Translational Oncology. 2024;40:101871.
doi:10.1016/j.tranon.2023.101871 .

Trivanović, Drenka, Mojsilović, Slavko, Bogosavljević, Nikola, Jurišić, Vladimir, Jauković, Aleksandra, "Revealing profile of cancer-educated platelets and their factors to foster immunotherapy development" in Translational Oncology, 40 (2024):101871,
https://doi.org/10.1016/j.tranon.2023.101871 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1346
AB  - Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T2  - Biomolecules
T1  - The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis
IS  - 10
SP  - 1437
VL  - 13
DO  - 10.3390/biom13101437
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Periodontitis (PD) is a degenerative, bacteria-induced chronic disease of periodontium causing bone resorption and teeth loss. It includes a strong reaction of immune cells through the secretion of proinflammatory factors such as Interleukin-17 (IL-17). PD treatment may consider systemic oral antibiotics application, including doxycycline (Dox), exhibiting antibacterial and anti-inflammatory properties along with supportive activity in wound healing, thus affecting alveolar bone metabolism. In the present study, we aimed to determine whether Dox can affect the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) modulated by IL-17 in terms of cell migration, osteogenic potential, bioenergetics and expression of extracellular matrix metalloproteinase 2 (MMP-2). Our findings indicate that Dox reduces the stimulatory effect of IL-17 on migration and MMP-2 expression in PDLSCs. Furthermore, Dox stimulates osteogenic differentiation of PDLSCs, annulling the inhibitory effect of IL-17 on PDLSCs osteogenesis. In addition, analyses of mitochondrial respiration reveal that Dox decreases oxygen consumption rate in PDLSCs exposed to IL-17, suggesting that changes in metabolic performance can be involved in Dox-mediated effects on PDLSCs. The pro-regenerative properties of Dox in inflammatory microenvironment candidates Dox in terms of regenerative therapy of PD-affected periodontium are observed.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules, Biomolecules",
title = "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis",
number = "10",
pages = "1437",
volume = "13",
doi = "10.3390/biom13101437"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 13(10), 1437.
https://doi.org/10.3390/biom13101437

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis. in Biomolecules. 2023;13(10):1437.
doi:10.3390/biom13101437 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "The Role of Doxycycline and IL-17 in Regenerative Potential of Periodontal Ligament Stem Cells: Implications in Periodontitis" in Biomolecules, 13, no. 10 (2023):1437,
https://doi.org/10.3390/biom13101437 . .

TY  - CONF
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Živanović, Milena
AU  - Momčilović, Sanja
AU  - Obradović, Hristina
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1406
AB  - Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.
PB  - Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije
C3  - Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
T1  - Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1406
ER  - 

@conference{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Živanović, Milena and Momčilović, Sanja and Obradović, Hristina and Petrović, Anđelija and Mojsilović, Slavko and Trivanović, Drenka and Jauković, Aleksandra",
year = "2023",
abstract = "Parodontopatija je hronično progresivno inflamatorno oboljenje tkiva parodoncijuma uzrokovano bakterijama zubnog plaka. Destrukcija parodoncijuma je posledica prekomerne aktivacije inflamatornog odgovora domaćina na bakterijsku infekciju i posledične produkcije citokina uključujući Interleukin-17 (IL-17). Lečenje parodontopatije podrazumeva i sistemsku primenu oralnih antibiotika poput doksociklina. Ovaj antibiotik iz klase tetraciklina ispoljava kako antibakterijska, tako i antiinflamatorna svojstva, a poznato je da utiče i na reparaciju tkiva i metabolizam alveolarnih kostiju. 
Cilj ove studije je bio da utvrdimo da li doksociklin utiče na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma (PDL-MMĆ) tretiranih sa IL-17. Naši rezultati su pokazali da doksociklin značajno smanjuje stimulativni efekat IL-17 na migraciju i ekspresiju matriksne metaloproteinaze 2 u PDL-MMĆ. Pored toga, doksociklin stimuliše osteogenu diferencijaciju PDL-MMĆ poništavajući inhibitorni efekat IL-17 na osteogenezu ovih ćelija. Analize ćelijske respiracije su otkrile da doksociklin smanjuje stopu potrošnje kiseonika i mitohondrijalnu biogenezu u IL-17-tretiranim PDL-MMĆ. Pokazana pro-regenerativna svojstva doksociklina u inflamatornom okruženju ukazuju na potencijal njegove primene u razvoju novih pristupa za terapiju parodontopatije.",
publisher = "Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije",
journal = "Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka",
title = "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1406"
}

Okić Đorđević, I., Kukolj, T., Živanović, M., Momčilović, S., Obradović, H., Petrović, A., Mojsilović, S., Trivanović, D.,& Jauković, A.. (2023). Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka
Beograd: Srpska akademija nauka i umetnosti, Odeljenje medicinskih nauka SANU, Odbor za imunologiju i alergologiju i Društvo imunologa Srbije..
https://hdl.handle.net/21.15107/rcub_rimi_1406

Okić Đorđević I, Kukolj T, Živanović M, Momčilović S, Obradović H, Petrović A, Mojsilović S, Trivanović D, Jauković A. Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma. in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka. 2023;.
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

Okić Đorđević, Ivana, Kukolj, Tamara, Živanović, Milena, Momčilović, Sanja, Obradović, Hristina, Petrović, Anđelija, Mojsilović, Slavko, Trivanović, Drenka, Jauković, Aleksandra, "Uticaj Doksiciklina na regenerativni potencijal mezenhimskih matičnih ćelija periodoncijuma" in Naučni skup Svetski dan imunologije, 27. april 2023. godine Svečana sala SANU - Knjiga sažetaka (2023),
https://hdl.handle.net/21.15107/rcub_rimi_1406 .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Vujačić, Marko
AU  - Arsić, Aleksandra
AU  - Bogosavljević, Nikola
AU  - Kovačić, Marijana
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Baščarević, Zoran
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1408
AB  - Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients
IS  - 3
SP  - P140
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1408
ER  - 

@conference{
author = "Trivanović, Drenka and Vujačić, Marko and Arsić, Aleksandra and Bogosavljević, Nikola and Kovačić, Marijana and Drvenica, Ivana and Mojsilović, Slavko and Baščarević, Zoran and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients",
number = "3",
pages = "P140",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1408"
}

Trivanović, D., Vujačić, M., Arsić, A., Bogosavljević, N., Kovačić, M., Drvenica, I., Mojsilović, S., Baščarević, Z., Bugarski, D.,& Jauković, A.. (2023). Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408

Trivanović D, Vujačić M, Arsić A, Bogosavljević N, Kovačić M, Drvenica I, Mojsilović S, Baščarević Z, Bugarski D, Jauković A. Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

Trivanović, Drenka, Vujačić, Marko, Arsić, Aleksandra, Bogosavljević, Nikola, Kovačić, Marijana, Drvenica, Ivana, Mojsilović, Slavko, Baščarević, Zoran, Bugarski, Diana, Jauković, Aleksandra, "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P140,
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Živanović, Milena
AU  - Vujačić, Marko
AU  - Bogosavljević, Nikola
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1409
AB  - Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation
IS  - 3
SP  - P141
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1409
ER  - 

@conference{
author = "Trivanović, Drenka and Okić Đorđević, Ivana and Živanović, Milena and Vujačić, Marko and Bogosavljević, Nikola and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation",
number = "3",
pages = "P141",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1409"
}

Trivanović, D., Okić Đorđević, I., Živanović, M., Vujačić, M., Bogosavljević, N., Bugarski, D.,& Jauković, A.. (2023). Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409

Trivanović D, Okić Đorđević I, Živanović M, Vujačić M, Bogosavljević N, Bugarski D, Jauković A. Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

Trivanović, Drenka, Okić Đorđević, Ivana, Živanović, Milena, Vujačić, Marko, Bogosavljević, Nikola, Bugarski, Diana, Jauković, Aleksandra, "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P141,
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Miletić, Maja
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1184
AB  - Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
PB  - Srpsko biološko društvo
T2  - Archives of Biological Sciences
T1  - Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
EP  - 24
IS  - 1
SP  - 15
VL  - 74
DO  - 10.2298/ABS210929048O
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Trivanović, Drenka and Petrović, Anđelija and Mojsilović, Slavko and Miletić, Maja and Bugarski, Diana and Jauković, Aleksandra",
year = "2022",
abstract = "Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.",
publisher = "Srpsko biološko društvo",
journal = "Archives of Biological Sciences",
title = "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway",
pages = "24-15",
number = "1",
volume = "74",
doi = "10.2298/ABS210929048O"
}

Okić Đorđević, I., Kukolj, T., Obradović, H., Trivanović, D., Petrović, A., Mojsilović, S., Miletić, M., Bugarski, D.,& Jauković, A.. (2022). Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences
Srpsko biološko društvo., 74(1), 15-24.
https://doi.org/10.2298/ABS210929048O

Okić Đorđević I, Kukolj T, Obradović H, Trivanović D, Petrović A, Mojsilović S, Miletić M, Bugarski D, Jauković A. Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences. 2022;74(1):15-24.
doi:10.2298/ABS210929048O .

Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Trivanović, Drenka, Petrović, Anđelija, Mojsilović, Slavko, Miletić, Maja, Bugarski, Diana, Jauković, Aleksandra, "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway" in Archives of Biological Sciences, 74, no. 1 (2022):15-24,
https://doi.org/10.2298/ABS210929048O . .

TY  - JOUR
AU  - Borojević, Ana
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Mojsilović, Slavko
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Živanović, Milena
AU  - Zečević, Željko
AU  - Simić, Marija
AU  - Gobeljić, Borko
AU  - Vujić, Dragana
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1207
AB  - The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T1  - Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
IS  - 2
SP  - 323
VL  - 12
DO  - 10.3390/biom12020323
ER  - 

@article{
author = "Borojević, Ana and Jauković, Aleksandra and Kukolj, Tamara and Mojsilović, Slavko and Obradović, Hristina and Trivanović, Drenka and Živanović, Milena and Zečević, Željko and Simić, Marija and Gobeljić, Borko and Vujić, Dragana and Bugarski, Diana",
year = "2022",
abstract = "The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules",
title = "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling",
number = "2",
pages = "323",
volume = "12",
doi = "10.3390/biom12020323"
}

Borojević, A., Jauković, A., Kukolj, T., Mojsilović, S., Obradović, H., Trivanović, D., Živanović, M., Zečević, Ž., Simić, M., Gobeljić, B., Vujić, D.,& Bugarski, D.. (2022). Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 323.
https://doi.org/10.3390/biom12020323

Borojević A, Jauković A, Kukolj T, Mojsilović S, Obradović H, Trivanović D, Živanović M, Zečević Ž, Simić M, Gobeljić B, Vujić D, Bugarski D. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules. 2022;12(2):323.
doi:10.3390/biom12020323 .

Borojević, Ana, Jauković, Aleksandra, Kukolj, Tamara, Mojsilović, Slavko, Obradović, Hristina, Trivanović, Drenka, Živanović, Milena, Zečević, Željko, Simić, Marija, Gobeljić, Borko, Vujić, Dragana, Bugarski, Diana, "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling" in Biomolecules, 12, no. 2 (2022):323,
https://doi.org/10.3390/biom12020323 . .

TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Jauković, Aleksandra
AU  - Milošević, Maja
AU  - Bugarski, Diana
AU  - Budeč, Mirela
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1241
AB  - Cancer-related anemia (CRA) is a common multifactorial disorder that adversely affects the quality of life and overall prognosis in patients with cancer. Safety concerns associated with the most common CRA treatment options, including intravenous iron therapy and erythropoietic-stimulating agents, have often resulted in no or suboptimal anemia management for many cancer patients. Chronic anemia creates a vital need to restore normal erythropoietic output and therefore activates the mechanisms of stress erythropoiesis (SE). A growing body of evidence demonstrates that bone morphogenetic protein 4 (BMP4) signaling, along with glucocorticoids, erythropoietin, stem cell factor, growth differentiation factor 15 (GDF15) and hypoxia-inducible factors, plays a pivotal role in SE. Nevertheless, a chronic state of SE may lead to ineffective erythropoiesis, characterized by the expansion of erythroid progenitor pool, that largely fails to differentiate and give rise to mature red blood cells, further aggravating CRA. In this review, we summarize the current state of knowledge on the emerging roles for stress erythroid progenitors and activated SE pathways in tumor progression, highlighting the urgent need to suppress ineffective erythropoiesis in cancer patients and develop an optimal treatment strategy as well as a personalized approach to CRA management.
PB  - Frontiers Media S.A
T2  - Frontiers in Physiology
T1  - Targeting Stress Erythropoiesis Pathways in Cancer
SP  - 844042
VL  - 13
DO  - 10.3389/fphys.2022.844042
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Jauković, Aleksandra and Milošević, Maja and Bugarski, Diana and Budeč, Mirela",
year = "2022",
abstract = "Cancer-related anemia (CRA) is a common multifactorial disorder that adversely affects the quality of life and overall prognosis in patients with cancer. Safety concerns associated with the most common CRA treatment options, including intravenous iron therapy and erythropoietic-stimulating agents, have often resulted in no or suboptimal anemia management for many cancer patients. Chronic anemia creates a vital need to restore normal erythropoietic output and therefore activates the mechanisms of stress erythropoiesis (SE). A growing body of evidence demonstrates that bone morphogenetic protein 4 (BMP4) signaling, along with glucocorticoids, erythropoietin, stem cell factor, growth differentiation factor 15 (GDF15) and hypoxia-inducible factors, plays a pivotal role in SE. Nevertheless, a chronic state of SE may lead to ineffective erythropoiesis, characterized by the expansion of erythroid progenitor pool, that largely fails to differentiate and give rise to mature red blood cells, further aggravating CRA. In this review, we summarize the current state of knowledge on the emerging roles for stress erythroid progenitors and activated SE pathways in tumor progression, highlighting the urgent need to suppress ineffective erythropoiesis in cancer patients and develop an optimal treatment strategy as well as a personalized approach to CRA management.",
publisher = "Frontiers Media S.A",
journal = "Frontiers in Physiology",
title = "Targeting Stress Erythropoiesis Pathways in Cancer",
pages = "844042",
volume = "13",
doi = "10.3389/fphys.2022.844042"
}

Vignjević-Petrinović, S., Jauković, A., Milošević, M., Bugarski, D.,& Budeč, M.. (2022). Targeting Stress Erythropoiesis Pathways in Cancer. in Frontiers in Physiology
Frontiers Media S.A., 13, 844042.
https://doi.org/10.3389/fphys.2022.844042

Vignjević-Petrinović S, Jauković A, Milošević M, Bugarski D, Budeč M. Targeting Stress Erythropoiesis Pathways in Cancer. in Frontiers in Physiology. 2022;13:844042.
doi:10.3389/fphys.2022.844042 .

Vignjević-Petrinović, Sanja, Jauković, Aleksandra, Milošević, Maja, Bugarski, Diana, Budeč, Mirela, "Targeting Stress Erythropoiesis Pathways in Cancer" in Frontiers in Physiology, 13 (2022):844042,
https://doi.org/10.3389/fphys.2022.844042 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Lazarević, Jasmina
AU  - Borojević, Ana
AU  - Ralević, Uroš
AU  - Vujić, Dragana
AU  - Jauković, Aleksandra
AU  - Lazarević, Nenad
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1235
AB  - The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
IS  - 9
SP  - 4915
VL  - 23
DO  - 10.3390/ijms23094915
ER  - 

@article{
author = "Kukolj, Tamara and Lazarević, Jasmina and Borojević, Ana and Ralević, Uroš and Vujić, Dragana and Jauković, Aleksandra and Lazarević, Nenad and Bugarski, Diana",
year = "2022",
abstract = "The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity",
number = "9",
pages = "4915",
volume = "23",
doi = "10.3390/ijms23094915"
}

Kukolj, T., Lazarević, J., Borojević, A., Ralević, U., Vujić, D., Jauković, A., Lazarević, N.,& Bugarski, D.. (2022). A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences
MDPI., 23(9), 4915.
https://doi.org/10.3390/ijms23094915

Kukolj T, Lazarević J, Borojević A, Ralević U, Vujić D, Jauković A, Lazarević N, Bugarski D. A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences. 2022;23(9):4915.
doi:10.3390/ijms23094915 .

Kukolj, Tamara, Lazarević, Jasmina, Borojević, Ana, Ralević, Uroš, Vujić, Dragana, Jauković, Aleksandra, Lazarević, Nenad, Bugarski, Diana, "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity" in International Journal of Molecular Sciences, 23, no. 9 (2022):4915,
https://doi.org/10.3390/ijms23094915 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Miletić, Maja
AU  - Petrović, Vanja
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1149
AB  - Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
PB  - Wiley-Blackwell
T2  - Journal of Cellular Physiology
T1  - Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
EP  - 7341
IS  - 11
SP  - 7322
VL  - 236
DO  - 10.1002/jcp.30399
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Obradović, Hristina and Miletić, Maja and Petrović, Vanja and Mojsilović, Slavko and Bugarski, Diana",
year = "2021",
abstract = "Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.",
publisher = "Wiley-Blackwell",
journal = "Journal of Cellular Physiology",
title = "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF",
pages = "7341-7322",
number = "11",
volume = "236",
doi = "10.1002/jcp.30399"
}

Jauković, A., Kukolj, T., Trivanović, D., Okić Đorđević, I., Obradović, H., Miletić, M., Petrović, V., Mojsilović, S.,& Bugarski, D.. (2021). Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology
Wiley-Blackwell., 236(11), 7322-7341.
https://doi.org/10.1002/jcp.30399

Jauković A, Kukolj T, Trivanović D, Okić Đorđević I, Obradović H, Miletić M, Petrović V, Mojsilović S, Bugarski D. Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology. 2021;236(11):7322-7341.
doi:10.1002/jcp.30399 .

Jauković, Aleksandra, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Obradović, Hristina, Miletić, Maja, Petrović, Vanja, Mojsilović, Slavko, Bugarski, Diana, "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF" in Journal of Cellular Physiology, 236, no. 11 (2021):7322-7341,
https://doi.org/10.1002/jcp.30399 . .

TY  - JOUR
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Petrović, Anđelija
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1175
AB  - As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Journal of Personalized Medicine
T1  - Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy
IS  - 11
SP  - 1133
VL  - 11
DO  - 10.3390/jpm11111133
ER  - 

@article{
author = "Mojsilović, Slavko and Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Petrović, Anđelija and Bugarski, Diana",
year = "2021",
abstract = "As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Journal of Personalized Medicine",
title = "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy",
number = "11",
pages = "1133",
volume = "11",
doi = "10.3390/jpm11111133"
}

Mojsilović, S., Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Petrović, A.,& Bugarski, D.. (2021). Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine
Multidisciplinary Digital Publishing Institute (MDPI)., 11(11), 1133.
https://doi.org/10.3390/jpm11111133

Mojsilović S, Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Petrović A, Bugarski D. Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine. 2021;11(11):1133.
doi:10.3390/jpm11111133 .

Mojsilović, Slavko, Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Petrović, Anđelija, Bugarski, Diana, "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy" in Journal of Personalized Medicine, 11, no. 11 (2021):1133,
https://doi.org/10.3390/jpm11111133 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Kukolj, Tamara
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1183
AB  - Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.
PB  - Baishideng Publishing Group Inc
T2  - World Journal of Stem Cells
T1  - Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy
EP  - 1880
IS  - 12
SP  - 1863
VL  - 13
DO  - 10.4252/wjsc.v13.i12.1863
ER  - 

@article{
author = "Okić Đorđević, Ivana and Obradović, Hristina and Kukolj, Tamara and Petrović, Anđelija and Mojsilović, Slavko and Bugarski, Diana and Jauković, Aleksandra",
year = "2021",
abstract = "Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.",
publisher = "Baishideng Publishing Group Inc",
journal = "World Journal of Stem Cells",
title = "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy",
pages = "1880-1863",
number = "12",
volume = "13",
doi = "10.4252/wjsc.v13.i12.1863"
}

Okić Đorđević, I., Obradović, H., Kukolj, T., Petrović, A., Mojsilović, S., Bugarski, D.,& Jauković, A.. (2021). Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells
Baishideng Publishing Group Inc., 13(12), 1863-1880.
https://doi.org/10.4252/wjsc.v13.i12.1863

Okić Đorđević I, Obradović H, Kukolj T, Petrović A, Mojsilović S, Bugarski D, Jauković A. Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells. 2021;13(12):1863-1880.
doi:10.4252/wjsc.v13.i12.1863 .

Okić Đorđević, Ivana, Obradović, Hristina, Kukolj, Tamara, Petrović, Anđelija, Mojsilović, Slavko, Bugarski, Diana, Jauković, Aleksandra, "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy" in World Journal of Stem Cells, 13, no. 12 (2021):1863-1880,
https://doi.org/10.4252/wjsc.v13.i12.1863 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/993
AB  - Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Stem Cells
T1  - Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators
EP  - 937
IS  - 9
SP  - 922
VL  - 12
DO  - 10.4252/wjsc.v12.i9.922
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Bugarski, Diana",
year = "2020",
abstract = "Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Stem Cells",
title = "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators",
pages = "937-922",
number = "9",
volume = "12",
doi = "10.4252/wjsc.v12.i9.922"
}

Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S.,& Bugarski, D.. (2020). Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells
Baishideng Publishing Group Inc, Pleasanton., 12(9), 922-937.
https://doi.org/10.4252/wjsc.v12.i9.922

Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Bugarski D. Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells. 2020;12(9):922-937.
doi:10.4252/wjsc.v12.i9.922 .

Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Bugarski, Diana, "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators" in World Journal of Stem Cells, 12, no. 9 (2020):922-937,
https://doi.org/10.4252/wjsc.v12.i9.922 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Vignjević-Petrinović, Sanja
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/995
AB  - Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cell & Developmental Biology
T1  - Adipogenesis in Different Body Depots and Tumor Development
VL  - 8
DO  - 10.3389/fcell.2020.571648
ER  - 

@article{
author = "Trivanović, Drenka and Vignjević-Petrinović, Sanja and Okić Đorđević, Ivana and Kukolj, Tamara and Bugarski, Diana and Jauković, Aleksandra",
year = "2020",
abstract = "Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cell & Developmental Biology",
title = "Adipogenesis in Different Body Depots and Tumor Development",
volume = "8",
doi = "10.3389/fcell.2020.571648"
}

Trivanović, D., Vignjević-Petrinović, S., Okić Đorđević, I., Kukolj, T., Bugarski, D.,& Jauković, A.. (2020). Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fcell.2020.571648

Trivanović D, Vignjević-Petrinović S, Okić Đorđević I, Kukolj T, Bugarski D, Jauković A. Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology. 2020;8.
doi:10.3389/fcell.2020.571648 .

Trivanović, Drenka, Vignjević-Petrinović, Sanja, Okić Đorđević, Ivana, Kukolj, Tamara, Bugarski, Diana, Jauković, Aleksandra, "Adipogenesis in Different Body Depots and Tumor Development" in Frontiers in Cell & Developmental Biology, 8 (2020),
https://doi.org/10.3389/fcell.2020.571648 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Abadjieva, Desislava
AU  - Trivanović, Drenka
AU  - Stoyanova, Elena
AU  - Kostadinova, Milena
AU  - Pashova, Shina
AU  - Kestendjieva, Snejana
AU  - Kukolj, Tamara
AU  - Ješeta, Michal
AU  - Kistanova, Elena
AU  - Mourdjeva, Milena
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1055
AB  - Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.
PB  - Springer, New York
T2  - Stem Cell Reviews & Reports
T1  - Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties
EP  - 875
IS  - 5
SP  - 853
VL  - 16
DO  - 10.1007/s12015-020-10006-9
ER  - 

@article{
author = "Jauković, Aleksandra and Abadjieva, Desislava and Trivanović, Drenka and Stoyanova, Elena and Kostadinova, Milena and Pashova, Shina and Kestendjieva, Snejana and Kukolj, Tamara and Ješeta, Michal and Kistanova, Elena and Mourdjeva, Milena",
year = "2020",
abstract = "Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.",
publisher = "Springer, New York",
journal = "Stem Cell Reviews & Reports",
title = "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties",
pages = "875-853",
number = "5",
volume = "16",
doi = "10.1007/s12015-020-10006-9"
}

Jauković, A., Abadjieva, D., Trivanović, D., Stoyanova, E., Kostadinova, M., Pashova, S., Kestendjieva, S., Kukolj, T., Ješeta, M., Kistanova, E.,& Mourdjeva, M.. (2020). Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports
Springer, New York., 16(5), 853-875.
https://doi.org/10.1007/s12015-020-10006-9

Jauković A, Abadjieva D, Trivanović D, Stoyanova E, Kostadinova M, Pashova S, Kestendjieva S, Kukolj T, Ješeta M, Kistanova E, Mourdjeva M. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports. 2020;16(5):853-875.
doi:10.1007/s12015-020-10006-9 .

Jauković, Aleksandra, Abadjieva, Desislava, Trivanović, Drenka, Stoyanova, Elena, Kostadinova, Milena, Pashova, Shina, Kestendjieva, Snejana, Kukolj, Tamara, Ješeta, Michal, Kistanova, Elena, Mourdjeva, Milena, "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties" in Stem Cell Reviews & Reports, 16, no. 5 (2020):853-875,
https://doi.org/10.1007/s12015-020-10006-9 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/950
AB  - Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.
PB  - Wiley, Hoboken
T2  - Cell Proliferation
T1  - IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells
IS  - 1
VL  - 52
DO  - 10.1111/cpr.12533
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Mojsilović, Slavko and Okić Đorđević, Ivana and Obradović, Hristina and Krstić, Jelena and Jauković, Aleksandra and Bugarski, Diana",
year = "2019",
abstract = "Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.",
publisher = "Wiley, Hoboken",
journal = "Cell Proliferation",
title = "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells",
number = "1",
volume = "52",
doi = "10.1111/cpr.12533"
}

Kukolj, T., Trivanović, D., Mojsilović, S., Okić Đorđević, I., Obradović, H., Krstić, J., Jauković, A.,& Bugarski, D.. (2019). IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation
Wiley, Hoboken., 52(1).
https://doi.org/10.1111/cpr.12533

Kukolj T, Trivanović D, Mojsilović S, Okić Đorđević I, Obradović H, Krstić J, Jauković A, Bugarski D. IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation. 2019;52(1).
doi:10.1111/cpr.12533 .

Kukolj, Tamara, Trivanović, Drenka, Mojsilović, Slavko, Okić Đorđević, Ivana, Obradović, Hristina, Krstić, Jelena, Jauković, Aleksandra, Bugarski, Diana, "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells" in Cell Proliferation, 52, no. 1 (2019),
https://doi.org/10.1111/cpr.12533 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić, Ivana
AU  - Kukolj, Tamara
AU  - Ilić, Vesna
AU  - Jauković, Aleksandra
AU  - Terzić, Milan
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/969
AB  - Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.
PB  - W B Saunders Co Ltd, London
T2  - Placenta
T1  - Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche
EP  - 34
SP  - 25
VL  - 82
DO  - 10.1016/j.placenta.2019.05.005
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Trivanović, Drenka and Mojsilović, Slavko and Okić, Ivana and Kukolj, Tamara and Ilić, Vesna and Jauković, Aleksandra and Terzić, Milan and Bugarski, Diana",
year = "2019",
abstract = "Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.",
publisher = "W B Saunders Co Ltd, London",
journal = "Placenta",
title = "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche",
pages = "34-25",
volume = "82",
doi = "10.1016/j.placenta.2019.05.005"
}

Obradović, H., Krstić, J., Trivanović, D., Mojsilović, S., Okić, I., Kukolj, T., Ilić, V., Jauković, A., Terzić, M.,& Bugarski, D.. (2019). Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta
W B Saunders Co Ltd, London., 82, 25-34.
https://doi.org/10.1016/j.placenta.2019.05.005

Obradović H, Krstić J, Trivanović D, Mojsilović S, Okić I, Kukolj T, Ilić V, Jauković A, Terzić M, Bugarski D. Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta. 2019;82:25-34.
doi:10.1016/j.placenta.2019.05.005 .

Obradović, Hristina, Krstić, Jelena, Trivanović, Drenka, Mojsilović, Slavko, Okić, Ivana, Kukolj, Tamara, Ilić, Vesna, Jauković, Aleksandra, Terzić, Milan, Bugarski, Diana, "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche" in Placenta, 82 (2019):25-34,
https://doi.org/10.1016/j.placenta.2019.05.005 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/835
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine & Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine & Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine & Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Obradović, Hristina
AU  - Janković, Srdja
AU  - Santibanez, Juan F.
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/873
AB  - Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (Escherichia coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability, and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP, and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPAR mRNA expression. LPS promoted myofibroblast-like phenotype of PDLSCs, since it significantly enhanced PDLSC contractility, as well as protein and/or gene expression of TGF-beta, fibronectin (FN), alpha-SMA, and NG2. LPS also increased protein and gene expression levels of anti-inflammatory COX-2 and pro-inflammatory IL-6 molecules in PDLSCs. Inhibition of peripheral blood mononuclear cells (MNCs) transendothelial migration in presence of LPS-treated PDLSCs was accompanied by the reduction of CD29 expression within MNCs. However, LPS treatment did not change the inhibitory effect of PDLSCs on mitogen-stimulated proliferation of CD4(+) and the ratio of CD4(+)CD25(high)/CD4(+)CD25(low) lymphocytes. LPS-treated PDLSCs did not change the frequency of CD34(+) and CD45(+) cells, but decreased the frequency of CD33(+) and CD14(+) myeloid cells within MNCs. Moreover, LPS treatment attenuated the stimulatory effect of PDLSCs on CFC activity of MNCs, predominantly the CFU-GM number. The results indicated that LPS-activated ERK1,2 was at least partly involved in the observed effects on PDLSC differentiation capacity, acquisition of myofibroblastic attributes, and changes of their immunomodulatory features.
PB  - Wiley, Hoboken
T2  - Journal of Cellular Physiology
T1  - Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling
EP  - 462
IS  - 1
SP  - 447
VL  - 233
DO  - 10.1002/jcp.25904
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Obradović, Hristina and Janković, Srdja and Santibanez, Juan F. and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (Escherichia coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability, and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP, and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPAR mRNA expression. LPS promoted myofibroblast-like phenotype of PDLSCs, since it significantly enhanced PDLSC contractility, as well as protein and/or gene expression of TGF-beta, fibronectin (FN), alpha-SMA, and NG2. LPS also increased protein and gene expression levels of anti-inflammatory COX-2 and pro-inflammatory IL-6 molecules in PDLSCs. Inhibition of peripheral blood mononuclear cells (MNCs) transendothelial migration in presence of LPS-treated PDLSCs was accompanied by the reduction of CD29 expression within MNCs. However, LPS treatment did not change the inhibitory effect of PDLSCs on mitogen-stimulated proliferation of CD4(+) and the ratio of CD4(+)CD25(high)/CD4(+)CD25(low) lymphocytes. LPS-treated PDLSCs did not change the frequency of CD34(+) and CD45(+) cells, but decreased the frequency of CD33(+) and CD14(+) myeloid cells within MNCs. Moreover, LPS treatment attenuated the stimulatory effect of PDLSCs on CFC activity of MNCs, predominantly the CFU-GM number. The results indicated that LPS-activated ERK1,2 was at least partly involved in the observed effects on PDLSC differentiation capacity, acquisition of myofibroblastic attributes, and changes of their immunomodulatory features.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling",
pages = "462-447",
number = "1",
volume = "233",
doi = "10.1002/jcp.25904"
}

Kukolj, T., Trivanović, D., Okić Đorđević, I., Mojsilović, S., Krstić, J., Obradović, H., Janković, S., Santibanez, J. F., Jauković, A.,& Bugarski, D.. (2018). Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. in Journal of Cellular Physiology
Wiley, Hoboken., 233(1), 447-462.
https://doi.org/10.1002/jcp.25904

Kukolj T, Trivanović D, Okić Đorđević I, Mojsilović S, Krstić J, Obradović H, Janković S, Santibanez JF, Jauković A, Bugarski D. Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. in Journal of Cellular Physiology. 2018;233(1):447-462.
doi:10.1002/jcp.25904 .

Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Obradović, Hristina, Janković, Srdja, Santibanez, Juan F., Jauković, Aleksandra, Bugarski, Diana, "Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling" in Journal of Cellular Physiology, 233, no. 1 (2018):447-462,
https://doi.org/10.1002/jcp.25904 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
AU  - Santibanez, Juan F.
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/871
AB  - Due to coexistence of stromal and epithelial tumor cells, their dynamic interactions have been widely recognized as significant cellular components to the tumor tissue integrity. Initiation and outcome of epithelial to mesenchymal transition (EMT) in tumor cells are dependent on their interaction with adjacent or recruited mesenchymal stromal cells (MSCs). A plethora of mechanisms are involved in MSCs-controlled employment of the developmental processes of EMT that contribute to loss of epithelial cell phenotype and acquisition of stemness, invasiveness and chemoresistance of tumor cells. Interplay of MSCs with tumor cells, including interchange of soluble biomolecules, plasma membrane structures, cytoplasmic content, and organelles, is established through cell-cell contact and/or by means of paracrine signaling. The main focus of this review is to summarize knowledge about involvement of MSCs in cancer cell EMT. Understanding the underlying cellular and molecular mechanism involved in the interplay between MSCs and cancer EMT is essential for development of effective therapy approaches, which in combination with current treatments may improve the control of tumor progression. Developmental Dynamics 247:359-367, 2018.
PB  - Wiley, Hoboken
T2  - Developmental Dynamics
T1  - Mesenchymal stromal cell engagement in cancer cell epithelial to mesenchymal transition
EP  - 367
IS  - 3
SP  - 359
VL  - 247
DO  - 10.1002/dvdy.24583
ER  - 

@article{
author = "Trivanović, Drenka and Krstić, Jelena and Jauković, Aleksandra and Bugarski, Diana and Santibanez, Juan F.",
year = "2018",
abstract = "Due to coexistence of stromal and epithelial tumor cells, their dynamic interactions have been widely recognized as significant cellular components to the tumor tissue integrity. Initiation and outcome of epithelial to mesenchymal transition (EMT) in tumor cells are dependent on their interaction with adjacent or recruited mesenchymal stromal cells (MSCs). A plethora of mechanisms are involved in MSCs-controlled employment of the developmental processes of EMT that contribute to loss of epithelial cell phenotype and acquisition of stemness, invasiveness and chemoresistance of tumor cells. Interplay of MSCs with tumor cells, including interchange of soluble biomolecules, plasma membrane structures, cytoplasmic content, and organelles, is established through cell-cell contact and/or by means of paracrine signaling. The main focus of this review is to summarize knowledge about involvement of MSCs in cancer cell EMT. Understanding the underlying cellular and molecular mechanism involved in the interplay between MSCs and cancer EMT is essential for development of effective therapy approaches, which in combination with current treatments may improve the control of tumor progression. Developmental Dynamics 247:359-367, 2018.",
publisher = "Wiley, Hoboken",
journal = "Developmental Dynamics",
title = "Mesenchymal stromal cell engagement in cancer cell epithelial to mesenchymal transition",
pages = "367-359",
number = "3",
volume = "247",
doi = "10.1002/dvdy.24583"
}

Trivanović, D., Krstić, J., Jauković, A., Bugarski, D.,& Santibanez, J. F.. (2018). Mesenchymal stromal cell engagement in cancer cell epithelial to mesenchymal transition. in Developmental Dynamics
Wiley, Hoboken., 247(3), 359-367.
https://doi.org/10.1002/dvdy.24583

Trivanović D, Krstić J, Jauković A, Bugarski D, Santibanez JF. Mesenchymal stromal cell engagement in cancer cell epithelial to mesenchymal transition. in Developmental Dynamics. 2018;247(3):359-367.
doi:10.1002/dvdy.24583 .

Trivanović, Drenka, Krstić, Jelena, Jauković, Aleksandra, Bugarski, Diana, Santibanez, Juan F., "Mesenchymal stromal cell engagement in cancer cell epithelial to mesenchymal transition" in Developmental Dynamics, 247, no. 3 (2018):359-367,
https://doi.org/10.1002/dvdy.24583 . .

TY  - JOUR
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Santibanez, Juan F.
AU  - Bugarski, Diana
PY  - 2017
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/768
AB  - In addition to providing essential molecules for the overall function of cells, metabolism plays an important role in cell fate and can be affected by microenvironmental stimuli as well as cellular interactions. As a specific niche, tumor microenvironment (TME), consisting of different cell types including stromal/stem cells and immune cells, is characterized by distinct metabolic properties. This review will be focused on the metabolic plasticity of mesenchymal stromal/stem cells (MSC) and macrophages in TME, as well as on how the metabolic state of cancer stem cells (CSC), as key drivers of oncogenesis, affects their generation and persistence. Namely, heterogenic metabolic phenotypes of these cell populations, which include various levels of dependence on glycolysis or oxidative phosphorylation are closely linked to their complex roles in cancer progression. Besides well-known extrinsic factors, such as cytokines and growth factors, the differentiation and activation states of CSC, MSC, and macrophages are coordinated by metabolic reprogramming in TME. The significance of mutual metabolic interaction between tumor stroma and cancer cells in the immune evasion and persistence of CSC is currently under investigation.
PB  - Frontiers Media Sa, Lausanne
T2  - FRONTIERS IN IMMUNOLOGY
T1  - Metabolic Plasticity of Stem Cells and Macrophages in Cancer
VL  - 8
DO  - 10.3389/fimmu.2017.00939
ER  - 

@article{
author = "Krstić, Jelena and Trivanović, Drenka and Jauković, Aleksandra and Santibanez, Juan F. and Bugarski, Diana",
year = "2017",
abstract = "In addition to providing essential molecules for the overall function of cells, metabolism plays an important role in cell fate and can be affected by microenvironmental stimuli as well as cellular interactions. As a specific niche, tumor microenvironment (TME), consisting of different cell types including stromal/stem cells and immune cells, is characterized by distinct metabolic properties. This review will be focused on the metabolic plasticity of mesenchymal stromal/stem cells (MSC) and macrophages in TME, as well as on how the metabolic state of cancer stem cells (CSC), as key drivers of oncogenesis, affects their generation and persistence. Namely, heterogenic metabolic phenotypes of these cell populations, which include various levels of dependence on glycolysis or oxidative phosphorylation are closely linked to their complex roles in cancer progression. Besides well-known extrinsic factors, such as cytokines and growth factors, the differentiation and activation states of CSC, MSC, and macrophages are coordinated by metabolic reprogramming in TME. The significance of mutual metabolic interaction between tumor stroma and cancer cells in the immune evasion and persistence of CSC is currently under investigation.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "FRONTIERS IN IMMUNOLOGY",
title = "Metabolic Plasticity of Stem Cells and Macrophages in Cancer",
volume = "8",
doi = "10.3389/fimmu.2017.00939"
}

Krstić, J., Trivanović, D., Jauković, A., Santibanez, J. F.,& Bugarski, D.. (2017). Metabolic Plasticity of Stem Cells and Macrophages in Cancer. in FRONTIERS IN IMMUNOLOGY
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fimmu.2017.00939

Krstić J, Trivanović D, Jauković A, Santibanez JF, Bugarski D. Metabolic Plasticity of Stem Cells and Macrophages in Cancer. in FRONTIERS IN IMMUNOLOGY. 2017;8.
doi:10.3389/fimmu.2017.00939 .

Krstić, Jelena, Trivanović, Drenka, Jauković, Aleksandra, Santibanez, Juan F., Bugarski, Diana, "Metabolic Plasticity of Stem Cells and Macrophages in Cancer" in FRONTIERS IN IMMUNOLOGY, 8 (2017),
https://doi.org/10.3389/fimmu.2017.00939 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Krstić, Jelena
AU  - Santibanez, Juan F.
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/707
AB  - Stromal vascular fraction of the adipose tissue is recognized as reservoir of the cell populations with stem and progenitor properties. Besides the roles of adipose tissue stem and progenitor cells (ASPCs) in adipose tissue development and homeostasis, particularly interest is given to their functions in adipose tissue expansion and obesity. Obesity-driven low-grade chronic inflammation, reduction of oxygen level in adipose tissue and engagement of bone marrow, support recruitment of various progenitor cells in adipose tissue. Activation of bone marrow-adipose tissue axis regulates hematopoiesis and infiltration of immune cells in adipose tissue. Obesity-associated factors can provoke alteration of ASPC multipotent differentiation and immunosuppressive potential. Determination of underpinning mechanisms in altered (obese) ASPC phenotype may contribute to development of novel approaches for treatment of metabolic disorders and advancement the safety and efficiency of adipose tissue grafting.
T2  - Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry
T1  - Obesity: An emerging importance of progenitors
EP  - 160
IS  - 3
SP  - 148
VL  - 16
DO  - 10.2174/1871522217666170105092757
ER  - 

@article{
author = "Trivanović, Drenka and Krstić, Jelena and Santibanez, Juan F. and Jauković, Aleksandra and Bugarski, Diana",
year = "2016",
abstract = "Stromal vascular fraction of the adipose tissue is recognized as reservoir of the cell populations with stem and progenitor properties. Besides the roles of adipose tissue stem and progenitor cells (ASPCs) in adipose tissue development and homeostasis, particularly interest is given to their functions in adipose tissue expansion and obesity. Obesity-driven low-grade chronic inflammation, reduction of oxygen level in adipose tissue and engagement of bone marrow, support recruitment of various progenitor cells in adipose tissue. Activation of bone marrow-adipose tissue axis regulates hematopoiesis and infiltration of immune cells in adipose tissue. Obesity-associated factors can provoke alteration of ASPC multipotent differentiation and immunosuppressive potential. Determination of underpinning mechanisms in altered (obese) ASPC phenotype may contribute to development of novel approaches for treatment of metabolic disorders and advancement the safety and efficiency of adipose tissue grafting.",
journal = "Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry",
title = "Obesity: An emerging importance of progenitors",
pages = "160-148",
number = "3",
volume = "16",
doi = "10.2174/1871522217666170105092757"
}

Trivanović, D., Krstić, J., Santibanez, J. F., Jauković, A.,& Bugarski, D.. (2016). Obesity: An emerging importance of progenitors. in Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry, 16(3), 148-160.
https://doi.org/10.2174/1871522217666170105092757

Trivanović D, Krstić J, Santibanez JF, Jauković A, Bugarski D. Obesity: An emerging importance of progenitors. in Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry. 2016;16(3):148-160.
doi:10.2174/1871522217666170105092757 .

Trivanović, Drenka, Krstić, Jelena, Santibanez, Juan F., Jauković, Aleksandra, Bugarski, Diana, "Obesity: An emerging importance of progenitors" in Immunology, Endocrine & Metabolic Agents in Medicinal Chemistry, 16, no. 3 (2016):148-160,
https://doi.org/10.2174/1871522217666170105092757 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Jovčić, Gordana
AU  - Bugarski, Diana
AU  - Santibanez, Juan F.
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/710
AB  - Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 inmyoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulateMMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.
PB  - Hindawi Ltd, London
T2  - Mediators of Inflammation
T1  - Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation
VL  - 2016
DO  - 10.1155/2016/2939658
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Mojsilović, Slavko and Jauković, Aleksandra and Jovčić, Gordana and Bugarski, Diana and Santibanez, Juan F.",
year = "2016",
abstract = "Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 inmyoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulateMMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17's capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17.",
publisher = "Hindawi Ltd, London",
journal = "Mediators of Inflammation",
title = "Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation",
volume = "2016",
doi = "10.1155/2016/2939658"
}

Obradović, H., Krstić, J., Kukolj, T., Trivanović, D., Okić Đorđević, I., Mojsilović, S., Jauković, A., Jovčić, G., Bugarski, D.,& Santibanez, J. F.. (2016). Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation. in Mediators of Inflammation
Hindawi Ltd, London., 2016.
https://doi.org/10.1155/2016/2939658

Obradović H, Krstić J, Kukolj T, Trivanović D, Okić Đorđević I, Mojsilović S, Jauković A, Jovčić G, Bugarski D, Santibanez JF. Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation. in Mediators of Inflammation. 2016;2016.
doi:10.1155/2016/2939658 .

Obradović, Hristina, Krstić, Jelena, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Mojsilović, Slavko, Jauković, Aleksandra, Jovčić, Gordana, Bugarski, Diana, Santibanez, Juan F., "Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation" in Mediators of Inflammation, 2016 (2016),
https://doi.org/10.1155/2016/2939658 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Jauković, Aleksandra
AU  - Krstić, Jelena
AU  - Nikolić, Srdjan
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Santibanez, Juan F.
AU  - Bugarski, Diana
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/726
AB  - Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-gamma (IFN-gamma) and/or tumor necrosis factor-alpha (TNF-alpha) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro. IFN-gamma and/or TNF-alpha-primed hASCs produced conditioned media (CM) which induced epithelial to mesenchymal transition (EMT) of MCF-7 cells by reducing E-Cadherin and increasing Vimentin expression. Induced EMT was accompanied by increased invasion, migration, and urokinase type-plasminogen activator (uPA) expression in MCF-7 cells. These effects were mediated by increased expression of transforming growth factor-beta 1(TGF-beta 1) in cytokines-primed hASCs, since inhibition of type I TGF-beta 1 receptor on MCF-7 cells and neutralization of TGF-beta 1 disabled the CM from primed hASCs to increase EMT, cell migration, and uPA expression in MCF-7 cells. Obtained data suggested that IFN-gamma and/or TNF-alpha primed hASCs might enhance the malignancy of MCF-7 cell line by inducing EMT, cell motility and uPA expression in these cells via TGF-beta 1-Smad3 signalization, with potentially important implications in breast cancer progression.
PB  - Wiley, Hoboken
T2  - Iubmb Life
T1  - Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1
EP  - 200
IS  - 3
SP  - 190
VL  - 68
DO  - 10.1002/iub.1473
ER  - 

@article{
author = "Trivanović, Drenka and Jauković, Aleksandra and Krstić, Jelena and Nikolić, Srdjan and Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Mojsilović, Slavko and Ilić, Vesna and Santibanez, Juan F. and Bugarski, Diana",
year = "2016",
abstract = "Mesenchymal stem cells from human adipose tissue (hASCs) are proposed as suitable tools for soft tissue engineering and reconstruction. Although it is known that hASCs have the ability to home to sites of inflammation and tumor niche, the role of inflammatory cytokines in the hASCs-affected tumor development is not understood. We found that interferon-gamma (IFN-gamma) and/or tumor necrosis factor-alpha (TNF-alpha) prime hASCs to produce soluble factors which enhance MCF-7 cell line malignancy in vitro. IFN-gamma and/or TNF-alpha-primed hASCs produced conditioned media (CM) which induced epithelial to mesenchymal transition (EMT) of MCF-7 cells by reducing E-Cadherin and increasing Vimentin expression. Induced EMT was accompanied by increased invasion, migration, and urokinase type-plasminogen activator (uPA) expression in MCF-7 cells. These effects were mediated by increased expression of transforming growth factor-beta 1(TGF-beta 1) in cytokines-primed hASCs, since inhibition of type I TGF-beta 1 receptor on MCF-7 cells and neutralization of TGF-beta 1 disabled the CM from primed hASCs to increase EMT, cell migration, and uPA expression in MCF-7 cells. Obtained data suggested that IFN-gamma and/or TNF-alpha primed hASCs might enhance the malignancy of MCF-7 cell line by inducing EMT, cell motility and uPA expression in these cells via TGF-beta 1-Smad3 signalization, with potentially important implications in breast cancer progression.",
publisher = "Wiley, Hoboken",
journal = "Iubmb Life",
title = "Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1",
pages = "200-190",
number = "3",
volume = "68",
doi = "10.1002/iub.1473"
}

Trivanović, D., Jauković, A., Krstić, J., Nikolić, S., Okić Đorđević, I., Kukolj, T., Obradović, H., Mojsilović, S., Ilić, V., Santibanez, J. F.,& Bugarski, D.. (2016). Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1. in Iubmb Life
Wiley, Hoboken., 68(3), 190-200.
https://doi.org/10.1002/iub.1473

Trivanović D, Jauković A, Krstić J, Nikolić S, Okić Đorđević I, Kukolj T, Obradović H, Mojsilović S, Ilić V, Santibanez JF, Bugarski D. Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1. in Iubmb Life. 2016;68(3):190-200.
doi:10.1002/iub.1473 .

Trivanović, Drenka, Jauković, Aleksandra, Krstić, Jelena, Nikolić, Srdjan, Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Mojsilović, Slavko, Ilić, Vesna, Santibanez, Juan F., Bugarski, Diana, "Inflammatory Cytokines Prime Adipose Tissue Mesenchymal Stem Cells to Enhance Malignancy of MCF-7 Breast Cancer Cells via Transforming Growth Factor-beta 1" in Iubmb Life, 68, no. 3 (2016):190-200,
https://doi.org/10.1002/iub.1473 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Obradović, Hristina
AU  - Santibanez, Juan F.
AU  - Mojsilović, Slavko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2016
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/731
AB  - Periodontal disease (PD), a degenerative bacterially induced disease of periodontium, can lead to bone resorption and teeth loss. Development of PD includes a strong inflammatory reaction, which involves multiple immune cells and their secreting factors including interleukin-17 (IL-17), which is not only an important modulator of immune and hematopoietic responses but also affects bone metabolism. In the present study we aimed to determine whether IL-17 affects the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) by investigating its ability to modulate osteogenic differentiation of these cells in vitro along with associated signaling pathways. Our results revealed that IL-17 inhibited both the proliferation and migration of PDLSCs and decreased their osteogenic differentiation by activating ERK1,2 and JNK mitogen-activated protein kinases. Obtained data suggested that IL-17 might contribute to alveolar bone loss in PD.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - International Journal of Biochemistry & Cell Biology
T1  - The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs
EP  - 101
SP  - 92
VL  - 71
DO  - 10.1016/j.biocel.2015.12.007
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Krstić, Jelena and Trivanović, Drenka and Obradović, Hristina and Santibanez, Juan F. and Mojsilović, Slavko and Ilić, Vesna and Bugarski, Diana and Jauković, Aleksandra",
year = "2016",
abstract = "Periodontal disease (PD), a degenerative bacterially induced disease of periodontium, can lead to bone resorption and teeth loss. Development of PD includes a strong inflammatory reaction, which involves multiple immune cells and their secreting factors including interleukin-17 (IL-17), which is not only an important modulator of immune and hematopoietic responses but also affects bone metabolism. In the present study we aimed to determine whether IL-17 affects the regenerative potential of periodontal ligament mesenchymal stem cells (PDLSCs) by investigating its ability to modulate osteogenic differentiation of these cells in vitro along with associated signaling pathways. Our results revealed that IL-17 inhibited both the proliferation and migration of PDLSCs and decreased their osteogenic differentiation by activating ERK1,2 and JNK mitogen-activated protein kinases. Obtained data suggested that IL-17 might contribute to alveolar bone loss in PD.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "International Journal of Biochemistry & Cell Biology",
title = "The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs",
pages = "101-92",
volume = "71",
doi = "10.1016/j.biocel.2015.12.007"
}

Okić Đorđević, I., Kukolj, T., Krstić, J., Trivanović, D., Obradović, H., Santibanez, J. F., Mojsilović, S., Ilić, V., Bugarski, D.,& Jauković, A.. (2016). The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs. in International Journal of Biochemistry & Cell Biology
Pergamon-Elsevier Science Ltd, Oxford., 71, 92-101.
https://doi.org/10.1016/j.biocel.2015.12.007

Okić Đorđević I, Kukolj T, Krstić J, Trivanović D, Obradović H, Santibanez JF, Mojsilović S, Ilić V, Bugarski D, Jauković A. The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs. in International Journal of Biochemistry & Cell Biology. 2016;71:92-101.
doi:10.1016/j.biocel.2015.12.007 .

Okić Đorđević, Ivana, Kukolj, Tamara, Krstić, Jelena, Trivanović, Drenka, Obradović, Hristina, Santibanez, Juan F., Mojsilović, Slavko, Ilić, Vesna, Bugarski, Diana, Jauković, Aleksandra, "The inhibition of periodontal ligament stem cells osteogenic differentiation by IL-17 is mediated via MAPKs" in International Journal of Biochemistry & Cell Biology, 71 (2016):92-101,
https://doi.org/10.1016/j.biocel.2015.12.007 . .