TY  - JOUR
AU  - Čokić, Vladan
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Kraguljac-Kurtović, Nada
AU  - Mojsilović, Sonja
AU  - Sefer, Dijana
AU  - Mitrović-Ajtić, Olivera
AU  - Milošević, Violeta
AU  - Bogdanović, Andrija
AU  - Đikić, Dragoslava
AU  - Milenković, Pavle B.
AU  - Puri, Raj K.
PY  - 2015
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/631
AB  - Purpose: We compared the gene expression profile of peripheral blood CD34(+) cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34(+) cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012 +/- SD of CD34(+) cells/mu l in peripheral blood. Results: The gene expression profile was more prominent in CML CD34(+) cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34(+) cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34(+) cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34(+) cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Blood Cells Molecules & Diseases
T1  - Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia
EP  - 381
IS  - 4
SP  - 373
VL  - 55
DO  - 10.1016/j.bcmd.2015.08.002
ER  - 

@article{
author = "Čokić, Vladan and Mojsilović, Slavko and Jauković, Aleksandra and Kraguljac-Kurtović, Nada and Mojsilović, Sonja and Sefer, Dijana and Mitrović-Ajtić, Olivera and Milošević, Violeta and Bogdanović, Andrija and Đikić, Dragoslava and Milenković, Pavle B. and Puri, Raj K.",
year = "2015",
abstract = "Purpose: We compared the gene expression profile of peripheral blood CD34(+) cells and granulocytes in subjects with chronic myeloid leukemia (CML), with the accent on signaling pathways affected by BCR-ABL oncogene. Methods: The microarray analyses have been performed in circulating CD34(+) cells and granulocytes from peripheral blood of 7 subjects with CML and 7 healthy donors. All studied BCR-ABL positive CML patients were in chronic phase, with a mean value of 2012 +/- SD of CD34(+) cells/mu l in peripheral blood. Results: The gene expression profile was more prominent in CML CD34(+) cells (3553 genes) compared to granulocytes (2701 genes). The 41 and 39 genes were significantly upregulated in CML CD34(+) cells (HINT1, TXN, SERBP1) and granulocytes, respectively. BCR-ABL oncogene activated PI3K/AKT and MAPK signaling through significant upregulation of PTPN11, CDK4/6, and MYC and reduction of E2F1, KRAS, and NFKBIA gene expression in CD34(+) cells. Among genes linked to the inhibition of cellular proliferation by BCR-ABL inhibitor Imatinib, the FOS and STAT1 demonstrated significantly decreased expression in CML. Conclusion: The presence of BCR-ABL fusion gene doubled the expression quantity of genes involved in the regulation of cell cycle, proliferation and apoptosis of CD34(+) cells. These results determined the modified genes in PI3K/AKT and MAPK signaling of CML subjects.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Blood Cells Molecules & Diseases",
title = "Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia",
pages = "381-373",
number = "4",
volume = "55",
doi = "10.1016/j.bcmd.2015.08.002"
}

Čokić, V., Mojsilović, S., Jauković, A., Kraguljac-Kurtović, N., Mojsilović, S., Sefer, D., Mitrović-Ajtić, O., Milošević, V., Bogdanović, A., Đikić, D., Milenković, P. B.,& Puri, R. K.. (2015). Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia. in Blood Cells Molecules & Diseases
Academic Press Inc Elsevier Science, San Diego., 55(4), 373-381.
https://doi.org/10.1016/j.bcmd.2015.08.002

Čokić V, Mojsilović S, Jauković A, Kraguljac-Kurtović N, Mojsilović S, Sefer D, Mitrović-Ajtić O, Milošević V, Bogdanović A, Đikić D, Milenković PB, Puri RK. Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia. in Blood Cells Molecules & Diseases. 2015;55(4):373-381.
doi:10.1016/j.bcmd.2015.08.002 .

Čokić, Vladan, Mojsilović, Slavko, Jauković, Aleksandra, Kraguljac-Kurtović, Nada, Mojsilović, Sonja, Sefer, Dijana, Mitrović-Ajtić, Olivera, Milošević, Violeta, Bogdanović, Andrija, Đikić, Dragoslava, Milenković, Pavle B., Puri, Raj K., "Gene expression profile of circulating CD34(+) cells and granulocytes in chronic myeloid leukemia" in Blood Cells Molecules & Diseases, 55, no. 4 (2015):373-381,
https://doi.org/10.1016/j.bcmd.2015.08.002 . .