TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Abadjieva, Desislava
AU  - Trivanović, Drenka
AU  - Stoyanova, Elena
AU  - Kostadinova, Milena
AU  - Pashova, Shina
AU  - Kestendjieva, Snejana
AU  - Kukolj, Tamara
AU  - Ješeta, Michal
AU  - Kistanova, Elena
AU  - Mourdjeva, Milena
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1055
AB  - Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.
PB  - Springer, New York
T2  - Stem Cell Reviews & Reports
T1  - Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties
EP  - 875
IS  - 5
SP  - 853
VL  - 16
DO  - 10.1007/s12015-020-10006-9
ER  - 

@article{
author = "Jauković, Aleksandra and Abadjieva, Desislava and Trivanović, Drenka and Stoyanova, Elena and Kostadinova, Milena and Pashova, Shina and Kestendjieva, Snejana and Kukolj, Tamara and Ješeta, Michal and Kistanova, Elena and Mourdjeva, Milena",
year = "2020",
abstract = "Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.",
publisher = "Springer, New York",
journal = "Stem Cell Reviews & Reports",
title = "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties",
pages = "875-853",
number = "5",
volume = "16",
doi = "10.1007/s12015-020-10006-9"
}

Jauković, A., Abadjieva, D., Trivanović, D., Stoyanova, E., Kostadinova, M., Pashova, S., Kestendjieva, S., Kukolj, T., Ješeta, M., Kistanova, E.,& Mourdjeva, M.. (2020). Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports
Springer, New York., 16(5), 853-875.
https://doi.org/10.1007/s12015-020-10006-9

Jauković A, Abadjieva D, Trivanović D, Stoyanova E, Kostadinova M, Pashova S, Kestendjieva S, Kukolj T, Ješeta M, Kistanova E, Mourdjeva M. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. in Stem Cell Reviews & Reports. 2020;16(5):853-875.
doi:10.1007/s12015-020-10006-9 .

Jauković, Aleksandra, Abadjieva, Desislava, Trivanović, Drenka, Stoyanova, Elena, Kostadinova, Milena, Pashova, Shina, Kestendjieva, Snejana, Kukolj, Tamara, Ješeta, Michal, Kistanova, Elena, Mourdjeva, Milena, "Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties" in Stem Cell Reviews & Reports, 16, no. 5 (2020):853-875,
https://doi.org/10.1007/s12015-020-10006-9 . .