TY  - JOUR
AU  - Kukolj, Tamara
AU  - Lazarević, Jasmina
AU  - Borojević, Ana
AU  - Ralević, Uroš
AU  - Vujić, Dragana
AU  - Jauković, Aleksandra
AU  - Lazarević, Nenad
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1235
AB  - The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
IS  - 9
SP  - 4915
VL  - 23
DO  - 10.3390/ijms23094915
ER  - 

@article{
author = "Kukolj, Tamara and Lazarević, Jasmina and Borojević, Ana and Ralević, Uroš and Vujić, Dragana and Jauković, Aleksandra and Lazarević, Nenad and Bugarski, Diana",
year = "2022",
abstract = "The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity",
number = "9",
pages = "4915",
volume = "23",
doi = "10.3390/ijms23094915"
}

Kukolj, T., Lazarević, J., Borojević, A., Ralević, U., Vujić, D., Jauković, A., Lazarević, N.,& Bugarski, D.. (2022). A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences
MDPI., 23(9), 4915.
https://doi.org/10.3390/ijms23094915

Kukolj T, Lazarević J, Borojević A, Ralević U, Vujić D, Jauković A, Lazarević N, Bugarski D. A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences. 2022;23(9):4915.
doi:10.3390/ijms23094915 .

Kukolj, Tamara, Lazarević, Jasmina, Borojević, Ana, Ralević, Uroš, Vujić, Dragana, Jauković, Aleksandra, Lazarević, Nenad, Bugarski, Diana, "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity" in International Journal of Molecular Sciences, 23, no. 9 (2022):4915,
https://doi.org/10.3390/ijms23094915 . .