TY  - JOUR
AU  - Simin, Stanislav
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Stefanov, Marijana
AU  - Savović, Milan
AU  - Gajić, Bojan
AU  - Lalošević, Vesna
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1477
AB  - The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Microorganisms
T1  - Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans
IS  - 2
SP  - 233
VL  - 12
DO  - 10.3390/microorganisms12020233
ER  - 

@article{
author = "Simin, Stanislav and Tomanović, Snežana and Sukara, Ratko and Stefanov, Marijana and Savović, Milan and Gajić, Bojan and Lalošević, Vesna",
year = "2024",
abstract = "The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Microorganisms",
title = "Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans",
number = "2",
pages = "233",
volume = "12",
doi = "10.3390/microorganisms12020233"
}

Simin, S., Tomanović, S., Sukara, R., Stefanov, M., Savović, M., Gajić, B.,& Lalošević, V.. (2024). Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans. in Microorganisms
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 233.
https://doi.org/10.3390/microorganisms12020233

Simin S, Tomanović S, Sukara R, Stefanov M, Savović M, Gajić B, Lalošević V. Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans. in Microorganisms. 2024;12(2):233.
doi:10.3390/microorganisms12020233 .

Simin, Stanislav, Tomanović, Snežana, Sukara, Ratko, Stefanov, Marijana, Savović, Milan, Gajić, Bojan, Lalošević, Vesna, "Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans" in Microorganisms, 12, no. 2 (2024):233,
https://doi.org/10.3390/microorganisms12020233 . .

TY  - JOUR
AU  - Kapo, Naida
AU  - Zuber Bogdanović, Ivana
AU  - Gagović, Ema
AU  - Žekić, Marina
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Mihaljica, Darko
AU  - Adžić, Bojan
AU  - Kadriaj, Përparim
AU  - Cvetkovikj, Aleksandar
AU  - Đađovski, Igor
AU  - Potkonjak, Aleksandar
AU  - Velo, Enkelejda
AU  - Savić, Sara
AU  - Tomanović, Snežana
AU  - Omeragić, Jasmin
AU  - Beck, Relja
AU  - Hodžić, Adnan
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1461
AB  - Ixodid ticks are distributed across all countries of the Western Balkans, with a high diversity of species. Many of these species serve as vectors of pathogens of veterinary and medical importance. Given the scattered data from Western Balkan countries, we have conducted a comprehensive review of available literature, including some historical data, with the aim to compile information about all recorded tick species and associated zoonotic pathogens in this region. Based on the collected data, the tick fauna of the Western Balkans encompasses 32 tick species belonging to five genera: Ixodes, Haemaphysalis, Dermacentor, Rhipicephalus and Hyalomma. A range of pathogens responsible for human diseases has also been documented, including viruses, bacteria and parasites. In this review, we emphasize the necessity for integrated surveillance and reporting, urging authorities to foster research by providing financial support. Additionally, international and interdisciplinary collaborations should be encouraged that include the exchange of expertise, experiences and resources. The present collaborative effort can effectively address gaps in our knowledge of ticks and tick-borne diseases.
PB  - Springer Nature
T2  - Parasites & Vectors
T2  - Parasites & VectorsParasites & Vectors
T1  - Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans
IS  - 1
SP  - 45
VL  - 17
DO  - 10.1186/s13071-023-06116-1
ER  - 

@article{
author = "Kapo, Naida and Zuber Bogdanović, Ivana and Gagović, Ema and Žekić, Marina and Veinović, Gorana and Sukara, Ratko and Mihaljica, Darko and Adžić, Bojan and Kadriaj, Përparim and Cvetkovikj, Aleksandar and Đađovski, Igor and Potkonjak, Aleksandar and Velo, Enkelejda and Savić, Sara and Tomanović, Snežana and Omeragić, Jasmin and Beck, Relja and Hodžić, Adnan",
year = "2024",
abstract = "Ixodid ticks are distributed across all countries of the Western Balkans, with a high diversity of species. Many of these species serve as vectors of pathogens of veterinary and medical importance. Given the scattered data from Western Balkan countries, we have conducted a comprehensive review of available literature, including some historical data, with the aim to compile information about all recorded tick species and associated zoonotic pathogens in this region. Based on the collected data, the tick fauna of the Western Balkans encompasses 32 tick species belonging to five genera: Ixodes, Haemaphysalis, Dermacentor, Rhipicephalus and Hyalomma. A range of pathogens responsible for human diseases has also been documented, including viruses, bacteria and parasites. In this review, we emphasize the necessity for integrated surveillance and reporting, urging authorities to foster research by providing financial support. Additionally, international and interdisciplinary collaborations should be encouraged that include the exchange of expertise, experiences and resources. The present collaborative effort can effectively address gaps in our knowledge of ticks and tick-borne diseases.",
publisher = "Springer Nature",
journal = "Parasites & Vectors, Parasites & VectorsParasites & Vectors",
title = "Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans",
number = "1",
pages = "45",
volume = "17",
doi = "10.1186/s13071-023-06116-1"
}

Kapo, N., Zuber Bogdanović, I., Gagović, E., Žekić, M., Veinović, G., Sukara, R., Mihaljica, D., Adžić, B., Kadriaj, P., Cvetkovikj, A., Đađovski, I., Potkonjak, A., Velo, E., Savić, S., Tomanović, S., Omeragić, J., Beck, R.,& Hodžić, A.. (2024). Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans. in Parasites & Vectors
Springer Nature., 17(1), 45.
https://doi.org/10.1186/s13071-023-06116-1

Kapo N, Zuber Bogdanović I, Gagović E, Žekić M, Veinović G, Sukara R, Mihaljica D, Adžić B, Kadriaj P, Cvetkovikj A, Đađovski I, Potkonjak A, Velo E, Savić S, Tomanović S, Omeragić J, Beck R, Hodžić A. Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans. in Parasites & Vectors. 2024;17(1):45.
doi:10.1186/s13071-023-06116-1 .

Kapo, Naida, Zuber Bogdanović, Ivana, Gagović, Ema, Žekić, Marina, Veinović, Gorana, Sukara, Ratko, Mihaljica, Darko, Adžić, Bojan, Kadriaj, Përparim, Cvetkovikj, Aleksandar, Đađovski, Igor, Potkonjak, Aleksandar, Velo, Enkelejda, Savić, Sara, Tomanović, Snežana, Omeragić, Jasmin, Beck, Relja, Hodžić, Adnan, "Ixodid ticks and zoonotic tick-borne pathogens of the Western Balkans" in Parasites & Vectors, 17, no. 1 (2024):45,
https://doi.org/10.1186/s13071-023-06116-1 . .

TY  - JOUR
AU  - Ćirković, Valentina
AU  - Veinović, Gorana
AU  - Stanković, Daliborka
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Tomanović, Snežana
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1465
AB  - Background: Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato, associated with lizards as reservoirs and Ixodes ricinus as its main vector. Borrelia lusitaniae is predominantly distributed in Central and Southeast Europe, and in countries of the Mediterranean basin, such as Portugal, Morocco, Tunisia, and Italy where this spirochete appears to infect vector ticks more frequently than other genospecies. Evolution of this zoonotic tick-borne microparasite is shaped by different environmental factors. Comprehensive phylogenetic analysis may give insight into how B. lusitaniae spreads to new geographic locations.  Aim: We applied Bayesian statistical methods to B. lusitaniae multilocus sequence typing (MLST) data to study the migration routes of B. lusitaniae and its potential for further spread.  Results: The discrete phylogeographic analysis placed origins of B. lusitaniae in Southeast Europe and identified at least two introductions of B. lusitaniae from Europe to North Africa. Estimated effective reproductive potential (Re), as a key indicator for a pathogen spread, suggested potential for further spread.  Conclusion: The results of this study can provide beneficial information about the potential for further spread of B. lusitaniae in Europe and North Africa and estimation of necessity for the development of strategies to monitor and control Lyme borreliosis.
PB  - Frontiers Media SA
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Evolutionary dynamics and geographical dispersal of Borrelia lusitaniae
SP  - 1330914
VL  - 15
DO  - 10.3389/fmicb.2024.1330914
ER  - 

@article{
author = "Ćirković, Valentina and Veinović, Gorana and Stanković, Daliborka and Mihaljica, Darko and Sukara, Ratko and Tomanović, Snežana",
year = "2024",
abstract = "Background: Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato, associated with lizards as reservoirs and Ixodes ricinus as its main vector. Borrelia lusitaniae is predominantly distributed in Central and Southeast Europe, and in countries of the Mediterranean basin, such as Portugal, Morocco, Tunisia, and Italy where this spirochete appears to infect vector ticks more frequently than other genospecies. Evolution of this zoonotic tick-borne microparasite is shaped by different environmental factors. Comprehensive phylogenetic analysis may give insight into how B. lusitaniae spreads to new geographic locations.  Aim: We applied Bayesian statistical methods to B. lusitaniae multilocus sequence typing (MLST) data to study the migration routes of B. lusitaniae and its potential for further spread.  Results: The discrete phylogeographic analysis placed origins of B. lusitaniae in Southeast Europe and identified at least two introductions of B. lusitaniae from Europe to North Africa. Estimated effective reproductive potential (Re), as a key indicator for a pathogen spread, suggested potential for further spread.  Conclusion: The results of this study can provide beneficial information about the potential for further spread of B. lusitaniae in Europe and North Africa and estimation of necessity for the development of strategies to monitor and control Lyme borreliosis.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Evolutionary dynamics and geographical dispersal of Borrelia lusitaniae",
pages = "1330914",
volume = "15",
doi = "10.3389/fmicb.2024.1330914"
}

Ćirković, V., Veinović, G., Stanković, D., Mihaljica, D., Sukara, R.,& Tomanović, S.. (2024). Evolutionary dynamics and geographical dispersal of Borrelia lusitaniae. in Frontiers in Microbiology
Frontiers Media SA., 15, 1330914.
https://doi.org/10.3389/fmicb.2024.1330914

Ćirković V, Veinović G, Stanković D, Mihaljica D, Sukara R, Tomanović S. Evolutionary dynamics and geographical dispersal of Borrelia lusitaniae. in Frontiers in Microbiology. 2024;15:1330914.
doi:10.3389/fmicb.2024.1330914 .

Ćirković, Valentina, Veinović, Gorana, Stanković, Daliborka, Mihaljica, Darko, Sukara, Ratko, Tomanović, Snežana, "Evolutionary dynamics and geographical dispersal of Borrelia lusitaniae" in Frontiers in Microbiology, 15 (2024):1330914,
https://doi.org/10.3389/fmicb.2024.1330914 . .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Andrić, Nenad
AU  - Francuski Andrić, Jelena
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Ranković, Vladan
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1270
AB  - Abstract Background  The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives  Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods  Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results  The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions  The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.
PB  - Wiley-Blackwell
T2  - Veterinary Medicine and Science
T1  - Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia
EP  - 118
IS  - 1
SP  - 111
VL  - 9
DO  - 10.1002/vms3.1061
ER  - 

@article{
author = "Sukara, Ratko and Andrić, Nenad and Francuski Andrić, Jelena and Mihaljica, Darko and Veinović, Gorana and Ranković, Vladan and Tomanović, Snežana",
year = "2023",
abstract = "Abstract Background  The epidemiological status concerning many canine tick-borne diseases (TBDs) in Serbia is still insufficiently known. Objectives  Our study aimed to investigate the presence of tick-borne pathogens of the family Anaplasmataceae and Hepatozoon spp., as a cause of illnesses accompanied by clinical signs that can occur in dogs with anaplasmosis, ehrlichiosis and hepatozoonosis. Methods  Dogs are included in the study based on the presence of a minimum of three clinical and/or pathological findings that could be associated with anaplasmosis, ehrlichiosis and hepatozoonosis. During the study (April–October 2018), 11 dogs met the conditions to be included in the survey. Identification of the causative agent in the blood of diseased dogs was performed by conventional PCR followed by sequencing. Results  The presence of the pathogens was confirmed in three animals (3/11, 27.3%). The presence of Ehrlichia canis was confirmed in 3-month-old female Rottweiler puppy, an 8-year old Miniature Schnauzer female was positive for Hepatozoon canis infection, while 4-year-old mixed breed male dog was co-infected with both mentioned pathogens. These are the first cases of autochthonous infection with E. canis and H. canis in dogs from Serbia confirmed by molecular methods. Conclusions  The results of our study indicate the importance of molecular methods to establish a reliable diagnosis of TBDs. Also, the confirmed presence of causative agents of canine monocytic ehrlichiosis and hepatozoonosis in Serbia appeals to veterinary practitioners that it is necessary to exclude the presence of those diseases in suspicious dogs.",
publisher = "Wiley-Blackwell",
journal = "Veterinary Medicine and Science",
title = "Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia",
pages = "118-111",
number = "1",
volume = "9",
doi = "10.1002/vms3.1061"
}

Sukara, R., Andrić, N., Francuski Andrić, J., Mihaljica, D., Veinović, G., Ranković, V.,& Tomanović, S.. (2023). Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia. in Veterinary Medicine and Science
Wiley-Blackwell., 9(1), 111-118.
https://doi.org/10.1002/vms3.1061

Sukara R, Andrić N, Francuski Andrić J, Mihaljica D, Veinović G, Ranković V, Tomanović S. Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia. in Veterinary Medicine and Science. 2023;9(1):111-118.
doi:10.1002/vms3.1061 .

Sukara, Ratko, Andrić, Nenad, Francuski Andrić, Jelena, Mihaljica, Darko, Veinović, Gorana, Ranković, Vladan, Tomanović, Snežana, "Autochthonous infection with Ehrlichia Canis and Hepatozoon Canis in dogs from Serbia" in Veterinary Medicine and Science, 9, no. 1 (2023):111-118,
https://doi.org/10.1002/vms3.1061 . .

TY  - CONF
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Vasić, Ana
AU  - Đurić Maslovara, I
AU  - Vukićević Radić, O
AU  - Rajković, M
AU  - Zlatić Sibinović, R
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1422
AB  - Introduction: Zoonotic tick-borne diseases (TBDs) represent an increasing threat in urban areas, 
including city parks and green recreational areas. Borrelia miyamotoi is pathogenic to humans but 
there is still little information about its circulation in nature and potential local impact on human health, while tick-borne encephalitis virus (TBEV) is endemic in many European countries and sporadic autochthonous cases in humans have been reported in Serbia.
The objective of our study was to determine the prevalence of two emerging zoonotic tick-borne 
pathogens (TBPs) − B. miyamotoi and TBEV in urban areas in the city of Belgrade (24 localities), 
four localities in Eastern Serbia, one in the province of the Vojvodina.
Methods: At 29 localities, a total of 480 ticks were collected from March to June 2021, and identified to the species level by using morphological keys. DNA and RNA were extracted from individual tick samples, while for molecular detection, probe-based qPCR and nested PCR were applied.Results: Among 480 ticks, 445 were identified as Ixodes ricinus (213 males, 176 females, 56 nymphs), 30 as Dermacentor reticulatus (18 females, 12 males), three Dermacentor marginatus females, one Haemaphysalis concinna female, and one Haemaphysalis punctata male. Using probe-based qPCR, the prevalence of B. miyamotoi in ticks was 1.04% (DNA was detected in five I. ricinus, two females and two males collected from three Belgrade localities and one male from Eastern Serbia), while sequencing was successful in samples of two I. ricinus females collected from two Belgrade localities and one male from Eastern Serbia. TBEV RNA was not detected in any sample. Conclusions: This is the first report on the presence of the emerging zoonotic pathogen B. miyamotoi in I. ricinus ticks in urban green areas in Serbia, indicating the risk of B. miyamotoi disease. Risk areas within cities should be identified and knowledge regarding TBPs and TBDs among the general population in urban areas should be increased.
C3  - 15th International Symposium on Ticks and Tick-borne Diseases 29–31 March 2023, Leonardo Hotel Weimar(Germany)
T1  - Molecular screening of Borrelia miyamotoi and Tick-Borne Encephalitis Virus in ixodid ticks in urban green areas in Serbia
SP  - P 087
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1422
ER  - 

@conference{
author = "Veinović, Gorana and Mihaljica, Darko and Sukara, Ratko and Vasić, Ana and Đurić Maslovara, I and Vukićević Radić, O and Rajković, M and Zlatić Sibinović, R and Tomanović, Snežana",
year = "2023",
abstract = "Introduction: Zoonotic tick-borne diseases (TBDs) represent an increasing threat in urban areas, 
including city parks and green recreational areas. Borrelia miyamotoi is pathogenic to humans but 
there is still little information about its circulation in nature and potential local impact on human health, while tick-borne encephalitis virus (TBEV) is endemic in many European countries and sporadic autochthonous cases in humans have been reported in Serbia.
The objective of our study was to determine the prevalence of two emerging zoonotic tick-borne 
pathogens (TBPs) − B. miyamotoi and TBEV in urban areas in the city of Belgrade (24 localities), 
four localities in Eastern Serbia, one in the province of the Vojvodina.
Methods: At 29 localities, a total of 480 ticks were collected from March to June 2021, and identified to the species level by using morphological keys. DNA and RNA were extracted from individual tick samples, while for molecular detection, probe-based qPCR and nested PCR were applied.Results: Among 480 ticks, 445 were identified as Ixodes ricinus (213 males, 176 females, 56 nymphs), 30 as Dermacentor reticulatus (18 females, 12 males), three Dermacentor marginatus females, one Haemaphysalis concinna female, and one Haemaphysalis punctata male. Using probe-based qPCR, the prevalence of B. miyamotoi in ticks was 1.04% (DNA was detected in five I. ricinus, two females and two males collected from three Belgrade localities and one male from Eastern Serbia), while sequencing was successful in samples of two I. ricinus females collected from two Belgrade localities and one male from Eastern Serbia. TBEV RNA was not detected in any sample. Conclusions: This is the first report on the presence of the emerging zoonotic pathogen B. miyamotoi in I. ricinus ticks in urban green areas in Serbia, indicating the risk of B. miyamotoi disease. Risk areas within cities should be identified and knowledge regarding TBPs and TBDs among the general population in urban areas should be increased.",
journal = "15th International Symposium on Ticks and Tick-borne Diseases 29–31 March 2023, Leonardo Hotel Weimar(Germany)",
title = "Molecular screening of Borrelia miyamotoi and Tick-Borne Encephalitis Virus in ixodid ticks in urban green areas in Serbia",
pages = "P 087",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1422"
}

Veinović, G., Mihaljica, D., Sukara, R., Vasić, A., Đurić Maslovara, I., Vukićević Radić, O., Rajković, M., Zlatić Sibinović, R.,& Tomanović, S.. (2023). Molecular screening of Borrelia miyamotoi and Tick-Borne Encephalitis Virus in ixodid ticks in urban green areas in Serbia. in 15th International Symposium on Ticks and Tick-borne Diseases 29–31 March 2023, Leonardo Hotel Weimar(Germany), P 087.
https://hdl.handle.net/21.15107/rcub_rimi_1422

Veinović G, Mihaljica D, Sukara R, Vasić A, Đurić Maslovara I, Vukićević Radić O, Rajković M, Zlatić Sibinović R, Tomanović S. Molecular screening of Borrelia miyamotoi and Tick-Borne Encephalitis Virus in ixodid ticks in urban green areas in Serbia. in 15th International Symposium on Ticks and Tick-borne Diseases 29–31 March 2023, Leonardo Hotel Weimar(Germany). 2023;:P 087.
https://hdl.handle.net/21.15107/rcub_rimi_1422 .

Veinović, Gorana, Mihaljica, Darko, Sukara, Ratko, Vasić, Ana, Đurić Maslovara, I, Vukićević Radić, O, Rajković, M, Zlatić Sibinović, R, Tomanović, Snežana, "Molecular screening of Borrelia miyamotoi and Tick-Borne Encephalitis Virus in ixodid ticks in urban green areas in Serbia" in 15th International Symposium on Ticks and Tick-borne Diseases 29–31 March 2023, Leonardo Hotel Weimar(Germany) (2023):P 087,
https://hdl.handle.net/21.15107/rcub_rimi_1422 .

TY  - CONF
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Vajić, Una Jovana
AU  - Petrović, Predrag
AU  - Božić, Dragana
AU  - Antić-Stanković, Jelena
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1423
PB  - Novi Sad: Poljoprivredni fakultet
C3  - XIV Simpozijum entomologa Srbije sa međunarodnim učešćem Novi Sad, 13-16. IX 2023 Poljoprivredni fakultet Univerziteta u Novom Sadu, Novi Sad
T1  - Antimikrobna aktivnost jaja i voska poreklom od jaja krpelja vrsta Ixodes ricinus i Dermacentor reticulatus
EP  - 54
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1423
ER  - 

@conference{
author = "Veinović, Gorana and Mihaljica, Darko and Sukara, Ratko and Vajić, Una Jovana and Petrović, Predrag and Božić, Dragana and Antić-Stanković, Jelena and Tomanović, Snežana",
year = "2023",
publisher = "Novi Sad: Poljoprivredni fakultet",
journal = "XIV Simpozijum entomologa Srbije sa međunarodnim učešćem Novi Sad, 13-16. IX 2023 Poljoprivredni fakultet Univerziteta u Novom Sadu, Novi Sad",
title = "Antimikrobna aktivnost jaja i voska poreklom od jaja krpelja vrsta Ixodes ricinus i Dermacentor reticulatus",
pages = "54-53",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1423"
}

Veinović, G., Mihaljica, D., Sukara, R., Vajić, U. J., Petrović, P., Božić, D., Antić-Stanković, J.,& Tomanović, S.. (2023). Antimikrobna aktivnost jaja i voska poreklom od jaja krpelja vrsta Ixodes ricinus i Dermacentor reticulatus. in XIV Simpozijum entomologa Srbije sa međunarodnim učešćem Novi Sad, 13-16. IX 2023 Poljoprivredni fakultet Univerziteta u Novom Sadu, Novi Sad
Novi Sad: Poljoprivredni fakultet., 53-54.
https://hdl.handle.net/21.15107/rcub_rimi_1423

Veinović G, Mihaljica D, Sukara R, Vajić UJ, Petrović P, Božić D, Antić-Stanković J, Tomanović S. Antimikrobna aktivnost jaja i voska poreklom od jaja krpelja vrsta Ixodes ricinus i Dermacentor reticulatus. in XIV Simpozijum entomologa Srbije sa međunarodnim učešćem Novi Sad, 13-16. IX 2023 Poljoprivredni fakultet Univerziteta u Novom Sadu, Novi Sad. 2023;:53-54.
https://hdl.handle.net/21.15107/rcub_rimi_1423 .

Veinović, Gorana, Mihaljica, Darko, Sukara, Ratko, Vajić, Una Jovana, Petrović, Predrag, Božić, Dragana, Antić-Stanković, Jelena, Tomanović, Snežana, "Antimikrobna aktivnost jaja i voska poreklom od jaja krpelja vrsta Ixodes ricinus i Dermacentor reticulatus" in XIV Simpozijum entomologa Srbije sa međunarodnim učešćem Novi Sad, 13-16. IX 2023 Poljoprivredni fakultet Univerziteta u Novom Sadu, Novi Sad (2023):53-54,
https://hdl.handle.net/21.15107/rcub_rimi_1423 .

TY  - CONF
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Božić Nedeljković, Biljana
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Vučetić, Dušan
AU  - Krstić, Milena
AU  - Mladenović, Jovan
AU  - Atanasievska, Sonja
AU  - Protić Đokić, Vesna
AU  - Đorđevski, Nikoleta
AU  - Ristanović, Elizabeta
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1446
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad
T1  - Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi
EP  - 71
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1446
ER  - 

@conference{
author = "Mihaljica, Darko and Marković, Dragana and Božić Nedeljković, Biljana and Veinović, Gorana and Sukara, Ratko and Vučetić, Dušan and Krstić, Milena and Mladenović, Jovan and Atanasievska, Sonja and Protić Đokić, Vesna and Đorđevski, Nikoleta and Ristanović, Elizabeta and Tomanović, Snežana",
year = "2023",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad",
title = "Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi",
pages = "71-70",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1446"
}

Mihaljica, D., Marković, D., Božić Nedeljković, B., Veinović, G., Sukara, R., Vučetić, D., Krstić, M., Mladenović, J., Atanasievska, S., Protić Đokić, V., Đorđevski, N., Ristanović, E.,& Tomanović, S.. (2023). Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 70-71.
https://hdl.handle.net/21.15107/rcub_rimi_1446

Mihaljica D, Marković D, Božić Nedeljković B, Veinović G, Sukara R, Vučetić D, Krstić M, Mladenović J, Atanasievska S, Protić Đokić V, Đorđevski N, Ristanović E, Tomanović S. Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad. 2023;:70-71.
https://hdl.handle.net/21.15107/rcub_rimi_1446 .

Mihaljica, Darko, Marković, Dragana, Božić Nedeljković, Biljana, Veinović, Gorana, Sukara, Ratko, Vučetić, Dušan, Krstić, Milena, Mladenović, Jovan, Atanasievska, Sonja, Protić Đokić, Vesna, Đorđevski, Nikoleta, Ristanović, Elizabeta, Tomanović, Snežana, "Detekcija markera uboda krpelja u serumu dobrovoljnih davaoca krvi" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani) - Zbornik kratkih sadržaja 24-26. april, 2023, Novi Sad (2023):70-71,
https://hdl.handle.net/21.15107/rcub_rimi_1446 .

TY  - CONF
AU  - Tomanović, Snežana
AU  - Veinović, Gorana
AU  - Malinić, Jovan
AU  - Sukara, Ratko
AU  - Mihaljica, Darko
AU  - Nikolić, Nataša
AU  - Katanić, Nataša
AU  - Poluga, Jasmina
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1447
AB  - Tropical Diseases of the University Clinical Centre of Serbia with a clinical presentation of disseminated erythema migrans. The patient and the mother could not recall if there had been a tick bite. A sample of blood was taken, and antibiotic therapy with amoxicillin was started immediately. Human serum sample was checked for the presence of IgM antibody against Borrelia burgdorferi sensu lato by commercial ELISA test and the sample was positive for IgM.
Blood was collected into the sterile K2EDTA tube, immediately transported to the Laboratory at the Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, and centrifuged twice at 2200 rpm for 17 min at room temperature. After centrifugation, one part of the serum was served for DNA extraction using ammonium hydroxide, ethanol, and sodium acetate while the sediment was inoculated into a 6 mL tube with Barbour-Stoenner-Kelly-H (BSK-H) medium under aseptic conditions and incubated as 33°C. After 16 days of incubation, viable, motile, and spiral-shaped microorganisms were observed in the initial BSK-H culture under dark-field microscopy, and incubation was prolonged for 29 days. DNA from the culture was extracted using centrifugation, dissolving the sediment in the water, and heating at 95°C for 10 minutes. After extracting DNA from the human serum and the culture, rrf-rrl rDNA intergenic spacer and flagellin gene (flaB) were amplified by conventional PCR, and sequencing of obtained PCR products was performed commercially (Macrogen, Amsterdam, the Netherlands).
After analysis of the sequences obtained, Borrelia lusitaniae was confirmed in human serum and culture. This is the first isolate of B. lusitaniae from a human blood sample that confirms that B. lusitaniae can disseminate via the hematogenous route.
C3  - International Symposium on TickBorne Pathogens and Disease ITPD 2023, 22 to 25 October 2023, Vienna, Austria - Book of Abstracts
T1  - Isolation of Borrelia lusitaniae from the blood of a patient with multiple erythema migrans
EP  - 111
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1447
ER  - 

@conference{
author = "Tomanović, Snežana and Veinović, Gorana and Malinić, Jovan and Sukara, Ratko and Mihaljica, Darko and Nikolić, Nataša and Katanić, Nataša and Poluga, Jasmina",
year = "2023",
abstract = "Tropical Diseases of the University Clinical Centre of Serbia with a clinical presentation of disseminated erythema migrans. The patient and the mother could not recall if there had been a tick bite. A sample of blood was taken, and antibiotic therapy with amoxicillin was started immediately. Human serum sample was checked for the presence of IgM antibody against Borrelia burgdorferi sensu lato by commercial ELISA test and the sample was positive for IgM.
Blood was collected into the sterile K2EDTA tube, immediately transported to the Laboratory at the Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, and centrifuged twice at 2200 rpm for 17 min at room temperature. After centrifugation, one part of the serum was served for DNA extraction using ammonium hydroxide, ethanol, and sodium acetate while the sediment was inoculated into a 6 mL tube with Barbour-Stoenner-Kelly-H (BSK-H) medium under aseptic conditions and incubated as 33°C. After 16 days of incubation, viable, motile, and spiral-shaped microorganisms were observed in the initial BSK-H culture under dark-field microscopy, and incubation was prolonged for 29 days. DNA from the culture was extracted using centrifugation, dissolving the sediment in the water, and heating at 95°C for 10 minutes. After extracting DNA from the human serum and the culture, rrf-rrl rDNA intergenic spacer and flagellin gene (flaB) were amplified by conventional PCR, and sequencing of obtained PCR products was performed commercially (Macrogen, Amsterdam, the Netherlands).
After analysis of the sequences obtained, Borrelia lusitaniae was confirmed in human serum and culture. This is the first isolate of B. lusitaniae from a human blood sample that confirms that B. lusitaniae can disseminate via the hematogenous route.",
journal = "International Symposium on TickBorne Pathogens and Disease ITPD 2023, 22 to 25 October 2023, Vienna, Austria - Book of Abstracts",
title = "Isolation of Borrelia lusitaniae from the blood of a patient with multiple erythema migrans",
pages = "111-111",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1447"
}

Tomanović, S., Veinović, G., Malinić, J., Sukara, R., Mihaljica, D., Nikolić, N., Katanić, N.,& Poluga, J.. (2023). Isolation of Borrelia lusitaniae from the blood of a patient with multiple erythema migrans. in International Symposium on TickBorne Pathogens and Disease ITPD 2023, 22 to 25 October 2023, Vienna, Austria - Book of Abstracts, 111-111.
https://hdl.handle.net/21.15107/rcub_rimi_1447

Tomanović S, Veinović G, Malinić J, Sukara R, Mihaljica D, Nikolić N, Katanić N, Poluga J. Isolation of Borrelia lusitaniae from the blood of a patient with multiple erythema migrans. in International Symposium on TickBorne Pathogens and Disease ITPD 2023, 22 to 25 October 2023, Vienna, Austria - Book of Abstracts. 2023;:111-111.
https://hdl.handle.net/21.15107/rcub_rimi_1447 .

Tomanović, Snežana, Veinović, Gorana, Malinić, Jovan, Sukara, Ratko, Mihaljica, Darko, Nikolić, Nataša, Katanić, Nataša, Poluga, Jasmina, "Isolation of Borrelia lusitaniae from the blood of a patient with multiple erythema migrans" in International Symposium on TickBorne Pathogens and Disease ITPD 2023, 22 to 25 October 2023, Vienna, Austria - Book of Abstracts (2023):111-111,
https://hdl.handle.net/21.15107/rcub_rimi_1447 .

TY  - CONF
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Tomanović, Snežana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1451
AB  - Kompleks Borrelia burgdorferi sensu lato trenutno obuhvata dvadest i jednu opisanu vrstu borelija a
karakteriše ih značajna varijabilnost u pogledu geografske distribucije kao i specifičnosti na relaciji
domaćini rezervoari- kompetentni vektor. Poznato je da samo određeni sojevi vrsta borelija izazivaju
bolest kod ljudi. U Severnoj Americi, lajmsku boreliozu uzrokuje samo jedna vrsta borelija-Borrelia
burgdorferi sensu stricto (s.s.), dok u Evropi infekciju kod ljudi može uzrokovati nekoliko različitih vrsta
borelija-Borrelia afzelii, Borrelia garinii, Borrelia bavariensis, Borrelia spielmanii i Borrelia burgdorferi s.s.
Vrste Borrelia valaisiana, Borrelia lusitaniae i Borrelia bissettii identifikovane su samo u pojedinačnim
slučajevima i prepoznate su kao potencijalni patogeni. Različite vrste borelija povezane su sa različitim
kliničkim manifestacijama lajmske borelioze; B. afzelii obično ostaje lokalizovana u koži, B. garinii i
B. bavariensis se obično povezuju s poremećajima nervnog sistema, dok se B. burgdorferi s.s. često
povezuje sa razvojem artritisa. Iako postoje registrovani slučajevi lajmske borelioze u Srbiji, ne postoje
podaci o tome koje vrste borelija izazivaju određene kliničke manifestacije lajmske borelioze.
Region Srbije karakteriše visoka rasprostranjenost i raznovrsnost vrsta borelija u krpeljima i njihovim
domaćinima. Istraživanja prisustva borelija u krpeljima kao i mikrobiološka izolacija sojeva borelija iz
krpelja u Srbiji, ukazala su na dominaciju vrste B. lusitaniae, slede vrste B. afzelii, B. bavariensis, B.
garinii, B. valaisiana i B. burgdorferi s.s. Takođe, B. lusitaniae je detektovana u krpeljima sakupljenim sa
guštera i u uzorcima slezine lisica, dok je B. valaisiana detektovana u krpeljima sakupljenim sa šakala
i ptica. Ispitivanje in vitro osetljivosti lokalnih sojeva borelija (izolovanih iz krpelja vrste Ixodes ricinus
sakupljenih sa vegetacije) na ljudski serum, pokazalo je da su sojevi B. valaisiana otporni na komplement,
dok su određeni sojevi B. lusitaniae (u prisustvu komplementa) podjednako pokretljvi kao i jedini
postojeći soj B. lusitaniae izolovan iz hronične kožne lezije, poreklom iz Portugala. Iako su lokalni sojevi
borelija osetljivi na antibiotike in vitro, ostaje nepoznato koje kliničke manifestacije lajmske borelioze
mogu izazvati vrste B. valaisiana i B. lusitaniae. Potrebno je da se razjasne mehanizami rezistencije
sojeva B. valaisiana i B. lusitaniae (koji cirkulišu u ovoj oblasti) u prisustvu komplementa.
AB  - The twenty-one described Borrelia species with considerable variability in host and vector associations,
and geographical distribution have been classified to the Borrelia burgdorferi sensu lato complex.
Only a subset of Borrelia species is known to cause human illness. In North America, Lyme borreliosis (LB)
is predominantly caused by the Borrelia burgdorferi sensu stricto (s.s.), while in Europe, the overall
diversity of Borrelia species is higher and LB can be caused by Borrelia afzelii, Borrelia garinii, Borrelia
bavariensis, Borrelia spielmanii, and Borrelia burgdorferi s.s., while Borrelia valaisiana, Borrelia lusitaniae
and Borrelia bissettii have been identified in single cases only and are recognized as potential
pathogens. Different Borrelia species are involved in distinct clinical manifestations of LB; B. afzelii
usually remains localized in the skin, B. garinii and B. bavariensis are usually associated with nervous
system disorders, while B. burgdorferi s.s. is commonly associated with development of the arthritis.
Although there are registered cases of LB in Serbia, there is no data on which Borrelia species cause
certain clinical manifestations of LB. Region of Serbia is characterized by high prevalence and diversity
of Borrelia species in ticks and their vertebrate hosts. The studies on the presence and isolation of Borrelia
from ticks from Serbia pointed to the domination of B. lusitaniae followed by B. afzelii, B. bavariensis,
B. garinii, B. valaisiana and B. burgdorferi s.s. Also, B. lusitaniae was detected in ticks collected
from lizards and in spleen samples collected from red foxes, while B. valaisiana was detected in ticks
collected from golden jackals and birds. In vitro susceptibility of local Borrelia strains isolated from
ticks to human complement, showed B. valaisiana strains resisted complement-mediated killing, while
certain B. lusitaniae strains (in the presence of complement) were equally motile as the only existing
one B. lusitaniae human isolate from Portugal. Although local Borrelia strains are susceptible to antibiotics
in vitro, it remains unknown what clinical manifestations of LB can be caused by B. valaisiana
and B. lusitaniae. It is needed to elucidate the mechanism behind B. valaisiana and B. lusitaniae strains
(circulating in the area) survival in the presence of human complement.
PB  - Belgrade: Serbian Society for Microbiology
C3  - 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
T1  - Borrelia lusitaniae i Borrelia valaisiana – potencijal za uzrokovanje lajmske borelioze u Srbiji
T1  - Borrelia lusitaniae and Borrelia valaisiana – potential for causing Lyme borreliosis in Serbia
EP  - 75
SP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1451
ER  - 

@conference{
author = "Veinović, Gorana and Mihaljica, Darko and Sukara, Ratko and Tomanović, Snežana",
year = "2023",
abstract = "Kompleks Borrelia burgdorferi sensu lato trenutno obuhvata dvadest i jednu opisanu vrstu borelija a
karakteriše ih značajna varijabilnost u pogledu geografske distribucije kao i specifičnosti na relaciji
domaćini rezervoari- kompetentni vektor. Poznato je da samo određeni sojevi vrsta borelija izazivaju
bolest kod ljudi. U Severnoj Americi, lajmsku boreliozu uzrokuje samo jedna vrsta borelija-Borrelia
burgdorferi sensu stricto (s.s.), dok u Evropi infekciju kod ljudi može uzrokovati nekoliko različitih vrsta
borelija-Borrelia afzelii, Borrelia garinii, Borrelia bavariensis, Borrelia spielmanii i Borrelia burgdorferi s.s.
Vrste Borrelia valaisiana, Borrelia lusitaniae i Borrelia bissettii identifikovane su samo u pojedinačnim
slučajevima i prepoznate su kao potencijalni patogeni. Različite vrste borelija povezane su sa različitim
kliničkim manifestacijama lajmske borelioze; B. afzelii obično ostaje lokalizovana u koži, B. garinii i
B. bavariensis se obično povezuju s poremećajima nervnog sistema, dok se B. burgdorferi s.s. često
povezuje sa razvojem artritisa. Iako postoje registrovani slučajevi lajmske borelioze u Srbiji, ne postoje
podaci o tome koje vrste borelija izazivaju određene kliničke manifestacije lajmske borelioze.
Region Srbije karakteriše visoka rasprostranjenost i raznovrsnost vrsta borelija u krpeljima i njihovim
domaćinima. Istraživanja prisustva borelija u krpeljima kao i mikrobiološka izolacija sojeva borelija iz
krpelja u Srbiji, ukazala su na dominaciju vrste B. lusitaniae, slede vrste B. afzelii, B. bavariensis, B.
garinii, B. valaisiana i B. burgdorferi s.s. Takođe, B. lusitaniae je detektovana u krpeljima sakupljenim sa
guštera i u uzorcima slezine lisica, dok je B. valaisiana detektovana u krpeljima sakupljenim sa šakala
i ptica. Ispitivanje in vitro osetljivosti lokalnih sojeva borelija (izolovanih iz krpelja vrste Ixodes ricinus
sakupljenih sa vegetacije) na ljudski serum, pokazalo je da su sojevi B. valaisiana otporni na komplement,
dok su određeni sojevi B. lusitaniae (u prisustvu komplementa) podjednako pokretljvi kao i jedini
postojeći soj B. lusitaniae izolovan iz hronične kožne lezije, poreklom iz Portugala. Iako su lokalni sojevi
borelija osetljivi na antibiotike in vitro, ostaje nepoznato koje kliničke manifestacije lajmske borelioze
mogu izazvati vrste B. valaisiana i B. lusitaniae. Potrebno je da se razjasne mehanizami rezistencije
sojeva B. valaisiana i B. lusitaniae (koji cirkulišu u ovoj oblasti) u prisustvu komplementa., The twenty-one described Borrelia species with considerable variability in host and vector associations,
and geographical distribution have been classified to the Borrelia burgdorferi sensu lato complex.
Only a subset of Borrelia species is known to cause human illness. In North America, Lyme borreliosis (LB)
is predominantly caused by the Borrelia burgdorferi sensu stricto (s.s.), while in Europe, the overall
diversity of Borrelia species is higher and LB can be caused by Borrelia afzelii, Borrelia garinii, Borrelia
bavariensis, Borrelia spielmanii, and Borrelia burgdorferi s.s., while Borrelia valaisiana, Borrelia lusitaniae
and Borrelia bissettii have been identified in single cases only and are recognized as potential
pathogens. Different Borrelia species are involved in distinct clinical manifestations of LB; B. afzelii
usually remains localized in the skin, B. garinii and B. bavariensis are usually associated with nervous
system disorders, while B. burgdorferi s.s. is commonly associated with development of the arthritis.
Although there are registered cases of LB in Serbia, there is no data on which Borrelia species cause
certain clinical manifestations of LB. Region of Serbia is characterized by high prevalence and diversity
of Borrelia species in ticks and their vertebrate hosts. The studies on the presence and isolation of Borrelia
from ticks from Serbia pointed to the domination of B. lusitaniae followed by B. afzelii, B. bavariensis,
B. garinii, B. valaisiana and B. burgdorferi s.s. Also, B. lusitaniae was detected in ticks collected
from lizards and in spleen samples collected from red foxes, while B. valaisiana was detected in ticks
collected from golden jackals and birds. In vitro susceptibility of local Borrelia strains isolated from
ticks to human complement, showed B. valaisiana strains resisted complement-mediated killing, while
certain B. lusitaniae strains (in the presence of complement) were equally motile as the only existing
one B. lusitaniae human isolate from Portugal. Although local Borrelia strains are susceptible to antibiotics
in vitro, it remains unknown what clinical manifestations of LB can be caused by B. valaisiana
and B. lusitaniae. It is needed to elucidate the mechanism behind B. valaisiana and B. lusitaniae strains
(circulating in the area) survival in the presence of human complement.",
publisher = "Belgrade: Serbian Society for Microbiology",
journal = "23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book",
title = "Borrelia lusitaniae i Borrelia valaisiana – potencijal za uzrokovanje lajmske borelioze u Srbiji, Borrelia lusitaniae and Borrelia valaisiana – potential for causing Lyme borreliosis in Serbia",
pages = "75-74",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1451"
}

Veinović, G., Mihaljica, D., Sukara, R.,& Tomanović, S.. (2023). Borrelia lusitaniae i Borrelia valaisiana – potencijal za uzrokovanje lajmske borelioze u Srbiji. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book
Belgrade: Serbian Society for Microbiology., 74-75.
https://hdl.handle.net/21.15107/rcub_rimi_1451

Veinović G, Mihaljica D, Sukara R, Tomanović S. Borrelia lusitaniae i Borrelia valaisiana – potencijal za uzrokovanje lajmske borelioze u Srbiji. in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book. 2023;:74-75.
https://hdl.handle.net/21.15107/rcub_rimi_1451 .

Veinović, Gorana, Mihaljica, Darko, Sukara, Ratko, Tomanović, Snežana, "Borrelia lusitaniae i Borrelia valaisiana – potencijal za uzrokovanje lajmske borelioze u Srbiji" in 23 UMS Series "Emerging infectious diseases: Are we ready for new evolutionary challenges?”, 30 March - 01 April, 2023, Belgrade, Serbia – E Abstract Book (2023):74-75,
https://hdl.handle.net/21.15107/rcub_rimi_1451 .

TY  - JOUR
AU  - Bilbija, Branka
AU  - Spitzweg, Cäcilia
AU  - Papoušek, Ivo
AU  - Fritz, Uwe
AU  - Földvári, Gábor
AU  - Mullett, Martin
AU  - Ihlow, Flora
AU  - Sprong, Hein
AU  - Civáňová Křížová, Kristína
AU  - Anisimov, Nikolay
AU  - Belova, Oxana A.
AU  - Bonnet, Sarah I.
AU  - Bychkova, Elizabeth
AU  - Czułowska, Aleksandra
AU  - Duscher, Georg G.
AU  - Fonville, Manoj
AU  - Kahl, Olaf
AU  - Karbowiak, Grzegorz
AU  - Kholodilov, Ivan S.
AU  - Kiewra, Dorota
AU  - Krčmar, Stjepan
AU  - Kumisbek, Gulzina
AU  - Livanova, Natalya
AU  - Majláth, Igor
AU  - Manfredi, Maria Teresa
AU  - Mihalca, Andrei D.
AU  - Miró, Guadalupe
AU  - Moutailler, Sara
AU  - Nebogatkin, Igor V.
AU  - Tomanović, Snežana
AU  - Vatansever, Zati
AU  - Yakovich, Marya
AU  - Zanzani, Sergio
AU  - Široký, Pavel
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1311
AB  - The ornate dog tick (Dermacentor reticulatus) shows a recently expanding geographic distribution. Knowledge on its intraspecific variability, population structure, rate of genetic diversity and divergence, including its evolution and geographic distribution, is crucial to understand its dispersal capacity. All such information would help to evaluate the potential risk of future spread of associated pathogens of medical and veterinary concern. A set of 865 D. reticulatus ticks was collected from 65 localities across 21 countries, from Portugal in the west to Kazakhstan and southern Russia in the east. Cluster analyses of 16 microsatellite loci were combined with nuclear (ITS2, 18S) and mitochondrial (12S, 16S, COI) sequence data to uncover the ticks’ population structures and geographical patterns. Approximate Bayesian computation was applied to model evolutionary relationships among the found clusters. Low variability and a weak phylogenetic signal showing an east–west cline were detected both for mitochondrial and nuclear sequence markers. Microsatellite analyses revealed three genetic clusters, where the eastern and western cluster gradient was supplemented by a third, northern cluster. Alternative scenarios could explain such a tripartite population structure by independent formation of clusters in separate refugia, limited gene flow connected with isolation by distance causing a “bipolar pattern”, and the northern cluster deriving from admixture between the eastern and western populations. The best supported demographic scenario of this tick species indicates that the northern cluster derived from admixture between the eastern and western populations 441 (median) to 224 (mode) generations ago, suggesting a possible link with the end of the Little Ice Age in Europe.
PB  - Elsevier
T2  - International Journal for Parasitology
T2  - International Journal for Parasitology
T1  - Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population
EP  - 101
IS  - 2
SP  - 91
VL  - 53
DO  - 10.1016/j.ijpara.2022.11.002
ER  - 

@article{
author = "Bilbija, Branka and Spitzweg, Cäcilia and Papoušek, Ivo and Fritz, Uwe and Földvári, Gábor and Mullett, Martin and Ihlow, Flora and Sprong, Hein and Civáňová Křížová, Kristína and Anisimov, Nikolay and Belova, Oxana A. and Bonnet, Sarah I. and Bychkova, Elizabeth and Czułowska, Aleksandra and Duscher, Georg G. and Fonville, Manoj and Kahl, Olaf and Karbowiak, Grzegorz and Kholodilov, Ivan S. and Kiewra, Dorota and Krčmar, Stjepan and Kumisbek, Gulzina and Livanova, Natalya and Majláth, Igor and Manfredi, Maria Teresa and Mihalca, Andrei D. and Miró, Guadalupe and Moutailler, Sara and Nebogatkin, Igor V. and Tomanović, Snežana and Vatansever, Zati and Yakovich, Marya and Zanzani, Sergio and Široký, Pavel",
year = "2023",
abstract = "The ornate dog tick (Dermacentor reticulatus) shows a recently expanding geographic distribution. Knowledge on its intraspecific variability, population structure, rate of genetic diversity and divergence, including its evolution and geographic distribution, is crucial to understand its dispersal capacity. All such information would help to evaluate the potential risk of future spread of associated pathogens of medical and veterinary concern. A set of 865 D. reticulatus ticks was collected from 65 localities across 21 countries, from Portugal in the west to Kazakhstan and southern Russia in the east. Cluster analyses of 16 microsatellite loci were combined with nuclear (ITS2, 18S) and mitochondrial (12S, 16S, COI) sequence data to uncover the ticks’ population structures and geographical patterns. Approximate Bayesian computation was applied to model evolutionary relationships among the found clusters. Low variability and a weak phylogenetic signal showing an east–west cline were detected both for mitochondrial and nuclear sequence markers. Microsatellite analyses revealed three genetic clusters, where the eastern and western cluster gradient was supplemented by a third, northern cluster. Alternative scenarios could explain such a tripartite population structure by independent formation of clusters in separate refugia, limited gene flow connected with isolation by distance causing a “bipolar pattern”, and the northern cluster deriving from admixture between the eastern and western populations. The best supported demographic scenario of this tick species indicates that the northern cluster derived from admixture between the eastern and western populations 441 (median) to 224 (mode) generations ago, suggesting a possible link with the end of the Little Ice Age in Europe.",
publisher = "Elsevier",
journal = "International Journal for Parasitology, International Journal for Parasitology",
title = "Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population",
pages = "101-91",
number = "2",
volume = "53",
doi = "10.1016/j.ijpara.2022.11.002"
}

Bilbija, B., Spitzweg, C., Papoušek, I., Fritz, U., Földvári, G., Mullett, M., Ihlow, F., Sprong, H., Civáňová Křížová, K., Anisimov, N., Belova, O. A., Bonnet, S. I., Bychkova, E., Czułowska, A., Duscher, G. G., Fonville, M., Kahl, O., Karbowiak, G., Kholodilov, I. S., Kiewra, D., Krčmar, S., Kumisbek, G., Livanova, N., Majláth, I., Manfredi, M. T., Mihalca, A. D., Miró, G., Moutailler, S., Nebogatkin, I. V., Tomanović, S., Vatansever, Z., Yakovich, M., Zanzani, S.,& Široký, P.. (2023). Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population. in International Journal for Parasitology
Elsevier., 53(2), 91-101.
https://doi.org/10.1016/j.ijpara.2022.11.002

Bilbija B, Spitzweg C, Papoušek I, Fritz U, Földvári G, Mullett M, Ihlow F, Sprong H, Civáňová Křížová K, Anisimov N, Belova OA, Bonnet SI, Bychkova E, Czułowska A, Duscher GG, Fonville M, Kahl O, Karbowiak G, Kholodilov IS, Kiewra D, Krčmar S, Kumisbek G, Livanova N, Majláth I, Manfredi MT, Mihalca AD, Miró G, Moutailler S, Nebogatkin IV, Tomanović S, Vatansever Z, Yakovich M, Zanzani S, Široký P. Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population. in International Journal for Parasitology. 2023;53(2):91-101.
doi:10.1016/j.ijpara.2022.11.002 .

Bilbija, Branka, Spitzweg, Cäcilia, Papoušek, Ivo, Fritz, Uwe, Földvári, Gábor, Mullett, Martin, Ihlow, Flora, Sprong, Hein, Civáňová Křížová, Kristína, Anisimov, Nikolay, Belova, Oxana A., Bonnet, Sarah I., Bychkova, Elizabeth, Czułowska, Aleksandra, Duscher, Georg G., Fonville, Manoj, Kahl, Olaf, Karbowiak, Grzegorz, Kholodilov, Ivan S., Kiewra, Dorota, Krčmar, Stjepan, Kumisbek, Gulzina, Livanova, Natalya, Majláth, Igor, Manfredi, Maria Teresa, Mihalca, Andrei D., Miró, Guadalupe, Moutailler, Sara, Nebogatkin, Igor V., Tomanović, Snežana, Vatansever, Zati, Yakovich, Marya, Zanzani, Sergio, Široký, Pavel, "Dermacentor reticulatus – a tick on its way from glacial refugia to a panmictic Eurasian population" in International Journal for Parasitology, 53, no. 2 (2023):91-101,
https://doi.org/10.1016/j.ijpara.2022.11.002 . .

TY  - JOUR
AU  - Vasić, Ana
AU  - Bjekić, Jovana
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Poluga, Jasmina
AU  - Filipović, Saša
AU  - Tomanović, Snežana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1213
AB  - This study assessed the level of knowledge, attitudes, and practices (KAP) regarding tick-borne encephalitis virus (TBEV) and tick-borne diseases (TBDs) among different groups of people in Serbia. Professionally tick-exposed persons (PTEPs), health care workers (HCWs), and the general population (GP) were subjected to an anonymous, voluntary, online questionnaire using Microsoft Forms. A total of 663 questionnaire responses were collected (February–March 2021), while 642 were included in the analysis. The significant difference in knowledge in TBDs existed between GP and PTEPs, and HCWs (p < 0.001). The perception of risk-to-tick exposure and TBDs was generally high (42.4 (95% CI: 33.6–51.2) within GP, 44.9 (95% CI: 35.8–53.9) within PTEPs and 46.2 (95% CI: 38.0–54.5) within HCWs), while fear was low (13.7 (95% CI: 7.9–19.5) within GP, 12.6 (95% CI: 7.3–19.9) within PTEPs, and 13.5 (95% CI: 7.4–19.5) within HCWs). Protective practices differed across groups (F (2639) = 12.920, p < 0.001, η2 = 0.039), with both PTEPs (t = 3.621, Cohen d = 0.332, p < 0.001) and HCWs (t = 4.644, Cohen d = 0.468, p < 0.001) adhering to more protective practices than the GP, without differences between PTEPs and HCWs (t = 1.256, Cohen d = 0.137, p = 0.421). Further education about TBDs in Serbia is required and critical points were identified in this study.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - International Journal of Environmental Research and Public Health
T1  - Knowledge, Attitudes, and Practices on Tick-Borne Encephalitis Virus and Tick-Borne Diseases within Professionally Tick-Exposed Persons, Health Care Workers, and General Population in Serbia: A Questionnaire-Based Study
IS  - 2
SP  - 867
VL  - 19
DO  - 10.3390/ijerph19020867
ER  - 

@article{
author = "Vasić, Ana and Bjekić, Jovana and Veinović, Gorana and Mihaljica, Darko and Sukara, Ratko and Poluga, Jasmina and Filipović, Saša and Tomanović, Snežana",
year = "2022",
abstract = "This study assessed the level of knowledge, attitudes, and practices (KAP) regarding tick-borne encephalitis virus (TBEV) and tick-borne diseases (TBDs) among different groups of people in Serbia. Professionally tick-exposed persons (PTEPs), health care workers (HCWs), and the general population (GP) were subjected to an anonymous, voluntary, online questionnaire using Microsoft Forms. A total of 663 questionnaire responses were collected (February–March 2021), while 642 were included in the analysis. The significant difference in knowledge in TBDs existed between GP and PTEPs, and HCWs (p < 0.001). The perception of risk-to-tick exposure and TBDs was generally high (42.4 (95% CI: 33.6–51.2) within GP, 44.9 (95% CI: 35.8–53.9) within PTEPs and 46.2 (95% CI: 38.0–54.5) within HCWs), while fear was low (13.7 (95% CI: 7.9–19.5) within GP, 12.6 (95% CI: 7.3–19.9) within PTEPs, and 13.5 (95% CI: 7.4–19.5) within HCWs). Protective practices differed across groups (F (2639) = 12.920, p < 0.001, η2 = 0.039), with both PTEPs (t = 3.621, Cohen d = 0.332, p < 0.001) and HCWs (t = 4.644, Cohen d = 0.468, p < 0.001) adhering to more protective practices than the GP, without differences between PTEPs and HCWs (t = 1.256, Cohen d = 0.137, p = 0.421). Further education about TBDs in Serbia is required and critical points were identified in this study.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "International Journal of Environmental Research and Public Health",
title = "Knowledge, Attitudes, and Practices on Tick-Borne Encephalitis Virus and Tick-Borne Diseases within Professionally Tick-Exposed Persons, Health Care Workers, and General Population in Serbia: A Questionnaire-Based Study",
number = "2",
pages = "867",
volume = "19",
doi = "10.3390/ijerph19020867"
}

Vasić, A., Bjekić, J., Veinović, G., Mihaljica, D., Sukara, R., Poluga, J., Filipović, S.,& Tomanović, S.. (2022). Knowledge, Attitudes, and Practices on Tick-Borne Encephalitis Virus and Tick-Borne Diseases within Professionally Tick-Exposed Persons, Health Care Workers, and General Population in Serbia: A Questionnaire-Based Study. in International Journal of Environmental Research and Public Health
Multidisciplinary Digital Publishing Institute (MDPI)., 19(2), 867.
https://doi.org/10.3390/ijerph19020867

Vasić A, Bjekić J, Veinović G, Mihaljica D, Sukara R, Poluga J, Filipović S, Tomanović S. Knowledge, Attitudes, and Practices on Tick-Borne Encephalitis Virus and Tick-Borne Diseases within Professionally Tick-Exposed Persons, Health Care Workers, and General Population in Serbia: A Questionnaire-Based Study. in International Journal of Environmental Research and Public Health. 2022;19(2):867.
doi:10.3390/ijerph19020867 .

Vasić, Ana, Bjekić, Jovana, Veinović, Gorana, Mihaljica, Darko, Sukara, Ratko, Poluga, Jasmina, Filipović, Saša, Tomanović, Snežana, "Knowledge, Attitudes, and Practices on Tick-Borne Encephalitis Virus and Tick-Borne Diseases within Professionally Tick-Exposed Persons, Health Care Workers, and General Population in Serbia: A Questionnaire-Based Study" in International Journal of Environmental Research and Public Health, 19, no. 2 (2022):867,
https://doi.org/10.3390/ijerph19020867 . .

TY  - JOUR
AU  - Kuručki, Milica
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Ćirović, Duško
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1334
AB  - Wild canids are globally recognised as hosts and reservoirs of a large number of ecto- and endoparasites. Data that reveal the importance of the grey wolf (Canis lupus L.1758) in the spread of hepatozoonosis are very scarce. There are a large number of different potential host species that can be infected by Hepatozoon canis, but the most common are domestic and wild carnivores, such as dogs, jackals, foxes, and wolves. In this study, the epidemiological significance of the grey wolf as a host for the pathogen was analysed for the first time in Serbia, as well as the genetic variability of H. canis. The presence of H. canis in wolf spleens has been demonstrated using molecular methods. A total of 107 wolf spleen samples from 30 localities in Serbia were analysed. The presence of H. canis was confirmed in 62 (57.94%) individuals from 26 out of 30 localities. According to the analysis, the sampled H. canis sequences were found to be characterised by a certain heterogeneity. Based on five mutated nucleotide sites in the sequences, H. canis could be divided into five sequence types, S1 to S5. The five sequence types can potentially circulate in grey wolf populations as well as among other domestic and wild canids. This study is the first confirmation of the presence of H. canis in grey wolf populations in Serbia. Considering that the role of this vector-borne disease is poorly researched in wild carnivores, it is very important to indicate the role of this species in the circulation of this pathogen in natural ecosystems.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Animals
T2  - Animals
T1  - High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia
IS  - 23
SP  - 3335
VL  - 12
DO  - 10.3390/ani12233335
ER  - 

@article{
author = "Kuručki, Milica and Tomanović, Snežana and Sukara, Ratko and Ćirović, Duško",
year = "2022",
abstract = "Wild canids are globally recognised as hosts and reservoirs of a large number of ecto- and endoparasites. Data that reveal the importance of the grey wolf (Canis lupus L.1758) in the spread of hepatozoonosis are very scarce. There are a large number of different potential host species that can be infected by Hepatozoon canis, but the most common are domestic and wild carnivores, such as dogs, jackals, foxes, and wolves. In this study, the epidemiological significance of the grey wolf as a host for the pathogen was analysed for the first time in Serbia, as well as the genetic variability of H. canis. The presence of H. canis in wolf spleens has been demonstrated using molecular methods. A total of 107 wolf spleen samples from 30 localities in Serbia were analysed. The presence of H. canis was confirmed in 62 (57.94%) individuals from 26 out of 30 localities. According to the analysis, the sampled H. canis sequences were found to be characterised by a certain heterogeneity. Based on five mutated nucleotide sites in the sequences, H. canis could be divided into five sequence types, S1 to S5. The five sequence types can potentially circulate in grey wolf populations as well as among other domestic and wild canids. This study is the first confirmation of the presence of H. canis in grey wolf populations in Serbia. Considering that the role of this vector-borne disease is poorly researched in wild carnivores, it is very important to indicate the role of this species in the circulation of this pathogen in natural ecosystems.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Animals, Animals",
title = "High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia",
number = "23",
pages = "3335",
volume = "12",
doi = "10.3390/ani12233335"
}

Kuručki, M., Tomanović, S., Sukara, R.,& Ćirović, D.. (2022). High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia. in Animals
Multidisciplinary Digital Publishing Institute (MDPI)., 12(23), 3335.
https://doi.org/10.3390/ani12233335

Kuručki M, Tomanović S, Sukara R, Ćirović D. High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia. in Animals. 2022;12(23):3335.
doi:10.3390/ani12233335 .

Kuručki, Milica, Tomanović, Snežana, Sukara, Ratko, Ćirović, Duško, "High Prevalence and Genetic Variability of Hepatozoon canis in Grey Wolf (Canis lupus L. 1758) Population in Serbia" in Animals, 12, no. 23 (2022):3335,
https://doi.org/10.3390/ani12233335 . .

TY  - JOUR
AU  - Mihaljica, Darko
AU  - Marković, Dragana
AU  - Repac, Jelena
AU  - Božić, Bojan
AU  - Radulović, Željko
AU  - Veinović, Gorana
AU  - Sukara, Ratko
AU  - Ristanović, Elizabeta
AU  - Chochlakis, Dimosthenis
AU  - Nedeljković Božić, Biljana 
AU  - Tomanović, Snežana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1160
AB  - In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.
PB  - Springer
T2  - Experimental and Applied Acarology
T1  - Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization
EP  - 99
IS  - 1
SP  - 83
VL  - 85
DO  - 10.1007/s10493-021-00653-z
ER  - 

@article{
author = "Mihaljica, Darko and Marković, Dragana and Repac, Jelena and Božić, Bojan and Radulović, Željko and Veinović, Gorana and Sukara, Ratko and Ristanović, Elizabeta and Chochlakis, Dimosthenis and Nedeljković Božić, Biljana  and Tomanović, Snežana",
year = "2021",
abstract = "In order to determine whether conserved tick salivary protein AV422 is immunogenic, the goal of our study was to detect specific IgG response within at-risk populations. Study groups included 76 individuals, differing in occurrence of recently recorded tick bites and health status. Western blotting with recombinant (r) protein derived from Ixodes ricinus (Ir) was performed. IgG response to Borrelia/Rickettsia, as indicators of previous tick infestations, was also assessed. Additionally, a detailed in silico AV422 protein sequence analysis was performed, followed by modelling of the interactions between peptides and corresponding MHC II molecules by molecular docking. Anti-rIrAV422 seroprevalences among individuals exposed to ticks were high (62.5, 57.9 and 66.7%) and anti-Borrelia/Rickettsia seroprevalences were 54.2, 15.8 and 44.4% among individuals with/without recent tick bite and patients suspected of tick-borne disease, respectively. In silico analysis of AV422 protein sequence showed a high level of conservation across tick genera, including also the predicted antigenic determinants specific for T and B cells. Docking to the restricted MHC II molecules was performed for all predicted AV422 T cell epitopes, and the most potent (highly immunogenic) epitope determinants were suggested. The epitope prediction reveals that tick salivary protein AV422 may elicit humoral immune response in humans, which is consistent with the high anti-rIrAV422 seroprevalence in tested at-risk subjects. Tick-borne diseases are a growing public health concern worldwide, and AV422 is potentially useful in clinical practice and epidemiological studies.",
publisher = "Springer",
journal = "Experimental and Applied Acarology",
title = "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization",
pages = "99-83",
number = "1",
volume = "85",
doi = "10.1007/s10493-021-00653-z"
}

Mihaljica, D., Marković, D., Repac, J., Božić, B., Radulović, Ž., Veinović, G., Sukara, R., Ristanović, E., Chochlakis, D., Nedeljković Božić, B.,& Tomanović, S.. (2021). Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology
Springer., 85(1), 83-99.
https://doi.org/10.1007/s10493-021-00653-z

Mihaljica D, Marković D, Repac J, Božić B, Radulović Ž, Veinović G, Sukara R, Ristanović E, Chochlakis D, Nedeljković Božić B, Tomanović S. Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization. in Experimental and Applied Acarology. 2021;85(1):83-99.
doi:10.1007/s10493-021-00653-z .

Mihaljica, Darko, Marković, Dragana, Repac, Jelena, Božić, Bojan, Radulović, Željko, Veinović, Gorana, Sukara, Ratko, Ristanović, Elizabeta, Chochlakis, Dimosthenis, Nedeljković Božić, Biljana , Tomanović, Snežana, "Exploring immunogenicity of tick salivary AV422 protein in persons exposed to ticks: prospects for utilization" in Experimental and Applied Acarology, 85, no. 1 (2021):83-99,
https://doi.org/10.1007/s10493-021-00653-z . .

TY  - JOUR
AU  - Veinović, Gorana
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ružić-Sabljić, Eva
AU  - Tomanović, Snežana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1415
AB  - In the present study, the effectiveness of six antimicrobial agents have been tested against 24 borrelia strains isolated from Ixodes ricinus ticks (11 Borrelia lusitaniae, eight Borrelia afzelii, three Borrelia garinii and two Borrelia valaisiana) and one B. lusitaniae strain isolated from human skin. The minimum inhibitory concentration range of antimicrobial agents was as follows: amoxicillin, 0.125–2 mg/L; doxycycline, 0.125–1 mg/L, ceftriaxone, 0.016–0.063 mg/L; cefuroxime, 0.063–1 mg/L; azithromycin, 0.0017–0.11 mg/L; amikacin 32–512 mg/L. Potentially pathogenic B. lusitaniae and B. valaisiana species were more susceptible to amoxicillin and azithromycin than pathogenic B. afzelii and B. garinii (P < 0.05); B. garinii, B. lusitaniae and B. valaisiana were more susceptible to doxycycline than B. afzelii (P < 0.05) while all species showed same susceptibility to ceftriaxone and cefuroxime (P > 0.05). This study is the first report on in vitro susceptibility of isolates from Serbia to antimicrobial agents and the first report on susceptibility of larger number of isolates of potentially pathogenic species B. lusitaniae. We showed that antimicrobial agents in vitro inhibit growth of borrelia strains very effectively, indicating the potential of their equally beneficial use in the treatment of Lyme borreliosis.
PB  - Akademiai Kiado
T2  - Acta Microbiologica Et Immunologica Hungarica
T1  - In vitro efficacy of antibiotics against different Borrelia isolates
EP  - 202
IS  - 3
SP  - 195
VL  - 68
DO  - 10.1556/030.2021.01441
ER  - 

@article{
author = "Veinović, Gorana and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Ružić-Sabljić, Eva and Tomanović, Snežana",
year = "2021",
abstract = "In the present study, the effectiveness of six antimicrobial agents have been tested against 24 borrelia strains isolated from Ixodes ricinus ticks (11 Borrelia lusitaniae, eight Borrelia afzelii, three Borrelia garinii and two Borrelia valaisiana) and one B. lusitaniae strain isolated from human skin. The minimum inhibitory concentration range of antimicrobial agents was as follows: amoxicillin, 0.125–2 mg/L; doxycycline, 0.125–1 mg/L, ceftriaxone, 0.016–0.063 mg/L; cefuroxime, 0.063–1 mg/L; azithromycin, 0.0017–0.11 mg/L; amikacin 32–512 mg/L. Potentially pathogenic B. lusitaniae and B. valaisiana species were more susceptible to amoxicillin and azithromycin than pathogenic B. afzelii and B. garinii (P < 0.05); B. garinii, B. lusitaniae and B. valaisiana were more susceptible to doxycycline than B. afzelii (P < 0.05) while all species showed same susceptibility to ceftriaxone and cefuroxime (P > 0.05). This study is the first report on in vitro susceptibility of isolates from Serbia to antimicrobial agents and the first report on susceptibility of larger number of isolates of potentially pathogenic species B. lusitaniae. We showed that antimicrobial agents in vitro inhibit growth of borrelia strains very effectively, indicating the potential of their equally beneficial use in the treatment of Lyme borreliosis.",
publisher = "Akademiai Kiado",
journal = "Acta Microbiologica Et Immunologica Hungarica",
title = "In vitro efficacy of antibiotics against different Borrelia isolates",
pages = "202-195",
number = "3",
volume = "68",
doi = "10.1556/030.2021.01441"
}

Veinović, G., Ćakić, S., Mihaljica, D., Sukara, R., Ružić-Sabljić, E.,& Tomanović, S.. (2021). In vitro efficacy of antibiotics against different Borrelia isolates. in Acta Microbiologica Et Immunologica Hungarica
Akademiai Kiado., 68(3), 195-202.
https://doi.org/10.1556/030.2021.01441

Veinović G, Ćakić S, Mihaljica D, Sukara R, Ružić-Sabljić E, Tomanović S. In vitro efficacy of antibiotics against different Borrelia isolates. in Acta Microbiologica Et Immunologica Hungarica. 2021;68(3):195-202.
doi:10.1556/030.2021.01441 .

Veinović, Gorana, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Ružić-Sabljić, Eva, Tomanović, Snežana, "In vitro efficacy of antibiotics against different Borrelia isolates" in Acta Microbiologica Et Immunologica Hungarica, 68, no. 3 (2021):195-202,
https://doi.org/10.1556/030.2021.01441 . .

TY  - CONF
AU  - Repac, Jelena
AU  - Mihaljica, Darko
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
AU  - Tomanović, Snežana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1416
PB  - Belgrade:Serbian Society for Parasitology
C3  - 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts
T1  - In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity
EP  - 112
SP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1416
ER  - 

@conference{
author = "Repac, Jelena and Mihaljica, Darko and Božić, Bojan and Božić Nedeljković, Biljana and Tomanović, Snežana",
year = "2021",
publisher = "Belgrade:Serbian Society for Parasitology",
journal = "13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts",
title = "In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity",
pages = "112-112",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1416"
}

Repac, J., Mihaljica, D., Božić, B., Božić Nedeljković, B.,& Tomanović, S.. (2021). In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity. in 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts
Belgrade:Serbian Society for Parasitology., 112-112.
https://hdl.handle.net/21.15107/rcub_rimi_1416

Repac J, Mihaljica D, Božić B, Božić Nedeljković B, Tomanović S. In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity. in 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts. 2021;:112-112.
https://hdl.handle.net/21.15107/rcub_rimi_1416 .

Repac, Jelena, Mihaljica, Darko, Božić, Bojan, Božić Nedeljković, Biljana, Tomanović, Snežana, "In silico characterization of the Ixodes ricinus AV422 salivary protein immunogenicity" in 13th European Multicolloquium of Parasitology- EMOP October 12-16, 2021, Belgrade - Programme & Book of Abstracts (2021):112-112,
https://hdl.handle.net/21.15107/rcub_rimi_1416 .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1002
AB  - Wild canids are recognized as important reservoir hosts for several vector-borne pathogens and are a good sentinel species for epidemiological surveillance. Identifying the reservoir hosts for particular zoonotic pathogens in a specific area enables the implementation of adequate measures in order to prevent the occurrence of zoonotic diseases in humans and domestic animals. The present review aims to summarize the role of wild canids in the epidemiology of tick-borne zoonoses in Serbia based on previously published papers and to discuss the available data from an epidemiological point of view. The results of previously published studies indicate that wild canids are potential reservoir hosts for several zoonotic tick-borne pathogens in Serbia, and hosts for several tick species which are proven competent vectors. Based on molecular studies conducted so far, the presence of Anaplasma phagocytophilum, was confirmed in jackals while the presence of Candidatus Neoehrlichia micurensis (FU98), Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia lusitaniae was proven by PCR in analyzed red foxes. There is a need for continuous epidemiological monitoring of diseases caused by pathogens previously confirmed in wild canids. Also, further research is needed to reveal the role of other wild and domestic animals in the epidemiology of tick-borne zoonoses in Serbia.
AB  - Divlje kanide su prepoznate kao važni domaćini rezervoari za nekoliko vektorima prenosivih zoonotskih patogena i predstavljaju dobre sentinel vrste za epidemiološki nadzor. Identifikacija domaćina rezervoara za pojedine zoonotske patogene na određenom području omogućava primenu odgovarajućih mera kako bi se sprečila pojava zoonoza kod ljudi i domaćih životinja. Cilj ove studije bio je da se predstavi uloga divljih kanida u epidemiologiji zoonoza koje se prenose vektorima u Srbiji na temelju prethodno objavljenih radova i da se dostupni podaci prodiskutuju sa epidemiološkog stanovišta. Na osnovu dosadašnjih molekularnih istraživanja u Srbiji, divlje kanide su prepoznate kao potencijalni rezervoari za nekoliko krpeljima prenosivih zoonotskih patogena. Kod šakala je potvrđeno prisustvo Anaplasma phagocytophilum, dok je prisustvo Candidatus Neoehrlichia micurensis (FU98), Borrelia burgdorferi sensu stricto, Borrelia garinii i Borrelia lusitaniae molekularnim metodama potvrđeno kod lisica. Rezultati pokazuju da su divlje kanide potencijalni domaćini rezervoari i izvor zoonotskih patogena u Srbiji i da predstavljaju dobre domaćine za više vrsta krpelja koji su potvrđeni kompetentni vektori pojedinih patogena. Potrebno je kontinuirano epidemiološko praćenje bolesti uzrokovanih patogenima čije je prisustvo prethodno potvrđeno kod divljih kanida. Takođe, potrebna su dodatna istraživanja kako bi se otkrila uloga drugih divljih i domaćih životinja u epidemiologiji zoonoza koje se prenose vektorima u našoj zemlji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia
T1  - Divlje kanide kao domaćini za vektorima prenosive zoonozne patogene u Srbiji
EP  - 153
IS  - 2
SP  - 144
VL  - 74
DO  - 10.2298/VETGL200810017S
ER  - 

@article{
author = "Sukara, Ratko and Mihaljica, Darko and Veinović, Gorana and Ćirović, Duško and Tomanović, Snežana",
year = "2020",
abstract = "Wild canids are recognized as important reservoir hosts for several vector-borne pathogens and are a good sentinel species for epidemiological surveillance. Identifying the reservoir hosts for particular zoonotic pathogens in a specific area enables the implementation of adequate measures in order to prevent the occurrence of zoonotic diseases in humans and domestic animals. The present review aims to summarize the role of wild canids in the epidemiology of tick-borne zoonoses in Serbia based on previously published papers and to discuss the available data from an epidemiological point of view. The results of previously published studies indicate that wild canids are potential reservoir hosts for several zoonotic tick-borne pathogens in Serbia, and hosts for several tick species which are proven competent vectors. Based on molecular studies conducted so far, the presence of Anaplasma phagocytophilum, was confirmed in jackals while the presence of Candidatus Neoehrlichia micurensis (FU98), Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia lusitaniae was proven by PCR in analyzed red foxes. There is a need for continuous epidemiological monitoring of diseases caused by pathogens previously confirmed in wild canids. Also, further research is needed to reveal the role of other wild and domestic animals in the epidemiology of tick-borne zoonoses in Serbia., Divlje kanide su prepoznate kao važni domaćini rezervoari za nekoliko vektorima prenosivih zoonotskih patogena i predstavljaju dobre sentinel vrste za epidemiološki nadzor. Identifikacija domaćina rezervoara za pojedine zoonotske patogene na određenom području omogućava primenu odgovarajućih mera kako bi se sprečila pojava zoonoza kod ljudi i domaćih životinja. Cilj ove studije bio je da se predstavi uloga divljih kanida u epidemiologiji zoonoza koje se prenose vektorima u Srbiji na temelju prethodno objavljenih radova i da se dostupni podaci prodiskutuju sa epidemiološkog stanovišta. Na osnovu dosadašnjih molekularnih istraživanja u Srbiji, divlje kanide su prepoznate kao potencijalni rezervoari za nekoliko krpeljima prenosivih zoonotskih patogena. Kod šakala je potvrđeno prisustvo Anaplasma phagocytophilum, dok je prisustvo Candidatus Neoehrlichia micurensis (FU98), Borrelia burgdorferi sensu stricto, Borrelia garinii i Borrelia lusitaniae molekularnim metodama potvrđeno kod lisica. Rezultati pokazuju da su divlje kanide potencijalni domaćini rezervoari i izvor zoonotskih patogena u Srbiji i da predstavljaju dobre domaćine za više vrsta krpelja koji su potvrđeni kompetentni vektori pojedinih patogena. Potrebno je kontinuirano epidemiološko praćenje bolesti uzrokovanih patogenima čije je prisustvo prethodno potvrđeno kod divljih kanida. Takođe, potrebna su dodatna istraživanja kako bi se otkrila uloga drugih divljih i domaćih životinja u epidemiologiji zoonoza koje se prenose vektorima u našoj zemlji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia, Divlje kanide kao domaćini za vektorima prenosive zoonozne patogene u Srbiji",
pages = "153-144",
number = "2",
volume = "74",
doi = "10.2298/VETGL200810017S"
}

Sukara, R., Mihaljica, D., Veinović, G., Ćirović, D.,& Tomanović, S.. (2020). Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(2), 144-153.
https://doi.org/10.2298/VETGL200810017S

Sukara R, Mihaljica D, Veinović G, Ćirović D, Tomanović S. Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia. in Veterinarski glasnik. 2020;74(2):144-153.
doi:10.2298/VETGL200810017S .

Sukara, Ratko, Mihaljica, Darko, Veinović, Gorana, Ćirović, Duško, Tomanović, Snežana, "Wild canids as hosts for ticks and tick-borne zoonotic pathogens in Serbia" in Veterinarski glasnik, 74, no. 2 (2020):144-153,
https://doi.org/10.2298/VETGL200810017S . .

TY  - JOUR
AU  - Veinović, Gorana
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Tomanović, Snežana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1044
AB  - Barbour-Stoenner-Kelly II (BSK-II) and BSK-H media were used for cultivation and isolation of fastidiousBorreliaspecies - the causative agents of Lyme borreliosis. Culture media have a limited shelf life and require adequate storage. Our goal was to assess how the growth ofBorreliawould be affected by prolonged storage of media and inadequate storage conditions (BSK-H stored at +4 degrees C for 2.5 years and BSK-II stored at -20 degrees C for 11 years). Growth of differentBorrelia afzelii,Borrelia garinii,Borrelia lusitaniaeandBorrelia valaisianastrains was assessed during 2 weeks of incubation at 33 degrees C. Monitored parameters included cell count per mL, morphology and motility. The results of this study have shown weaker growth of borrelia strains in BSK-H at +4 degrees C (median final cell number of 1.5 x 10(6)/mL) than in BSK-II at -20 degrees C (median final cell number of 7.75 x 10(6)/mL) and in fresh BSK-H media (median final cell number of 8.95 x 10(6)/mL). Duration of storage of media had no impact onBorreliamorphology and motility. Our results indicate that temperature of -20 degrees C is optimal for long-term storage of medium, BSK-II stored for 11 years provided effective support to growth ofBorreliaand may be employed for cultivation.
PB  - Wiley, Hoboken
T2  - APMIS
T1  - Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods
EP  - 557
IS  - 10
SP  - 552
VL  - 128
DO  - 10.1111/apm.13069
ER  - 

@article{
author = "Veinović, Gorana and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Tomanović, Snežana",
year = "2020",
abstract = "Barbour-Stoenner-Kelly II (BSK-II) and BSK-H media were used for cultivation and isolation of fastidiousBorreliaspecies - the causative agents of Lyme borreliosis. Culture media have a limited shelf life and require adequate storage. Our goal was to assess how the growth ofBorreliawould be affected by prolonged storage of media and inadequate storage conditions (BSK-H stored at +4 degrees C for 2.5 years and BSK-II stored at -20 degrees C for 11 years). Growth of differentBorrelia afzelii,Borrelia garinii,Borrelia lusitaniaeandBorrelia valaisianastrains was assessed during 2 weeks of incubation at 33 degrees C. Monitored parameters included cell count per mL, morphology and motility. The results of this study have shown weaker growth of borrelia strains in BSK-H at +4 degrees C (median final cell number of 1.5 x 10(6)/mL) than in BSK-II at -20 degrees C (median final cell number of 7.75 x 10(6)/mL) and in fresh BSK-H media (median final cell number of 8.95 x 10(6)/mL). Duration of storage of media had no impact onBorreliamorphology and motility. Our results indicate that temperature of -20 degrees C is optimal for long-term storage of medium, BSK-II stored for 11 years provided effective support to growth ofBorreliaand may be employed for cultivation.",
publisher = "Wiley, Hoboken",
journal = "APMIS",
title = "Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods",
pages = "557-552",
number = "10",
volume = "128",
doi = "10.1111/apm.13069"
}

Veinović, G., Ćakić, S., Mihaljica, D., Sukara, R.,& Tomanović, S.. (2020). Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods. in APMIS
Wiley, Hoboken., 128(10), 552-557.
https://doi.org/10.1111/apm.13069

Veinović G, Ćakić S, Mihaljica D, Sukara R, Tomanović S. Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods. in APMIS. 2020;128(10):552-557.
doi:10.1111/apm.13069 .

Veinović, Gorana, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Tomanović, Snežana, "Comparison of growth and morphology of Borrelia burgdorferi sensu lato in BSK-H and BSK-II media stored for prolonged periods" in APMIS, 128, no. 10 (2020):552-557,
https://doi.org/10.1111/apm.13069 . .

TY  - CONF
AU  - Veinović, Gorana
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Tomanović, Snežana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1414
PB  - Federation of European Microbiological Societies (FEMS)
C3  - FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book
T1  - Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis
SP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1414
ER  - 

@conference{
author = "Veinović, Gorana and Ćakić, Sanja and Mihaljica, Darko and Sukara, Ratko and Tomanović, Snežana",
year = "2020",
publisher = "Federation of European Microbiological Societies (FEMS)",
journal = "FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book",
title = "Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis",
pages = "220",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1414"
}

Veinović, G., Ćakić, S., Mihaljica, D., Sukara, R.,& Tomanović, S.. (2020). Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis. in FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book
Federation of European Microbiological Societies (FEMS)., 220.
https://hdl.handle.net/21.15107/rcub_rimi_1414

Veinović G, Ćakić S, Mihaljica D, Sukara R, Tomanović S. Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis. in FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book. 2020;:220.
https://hdl.handle.net/21.15107/rcub_rimi_1414 .

Veinović, Gorana, Ćakić, Sanja, Mihaljica, Darko, Sukara, Ratko, Tomanović, Snežana, "Evaluation of in vitro antibiotic efficacy against local strains of Borrelia burgdorferi sensu lato - the causative agents of Lyme borreliosis" in FEMS (Federation of European Microbiological Societies) online conference on microbiology, October 28-31, 2020 - Electronic abstract book (2020):220,
https://hdl.handle.net/21.15107/rcub_rimi_1414 .

Potkonjak, A., Savić, S., Spasojević-Kosić, L., Tasić-Otašević, S., Tomanović, S.,& Kovačević-Filipović, M.. (2020). Consensus statement on the epidemiological situation and expected frequency of canine vector-borne diseases in Serbia. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(2), 211-215.
https://doi.org/10.2298/VETGL201124016P

Potkonjak A, Savić S, Spasojević-Kosić L, Tasić-Otašević S, Tomanović S, Kovačević-Filipović M. Consensus statement on the epidemiological situation and expected frequency of canine vector-borne diseases in Serbia. in Veterinarski glasnik. 2020;74(2):211-215.
doi:10.2298/VETGL201124016P .

Potkonjak, Aleksandar, Savić, Sara, Spasojević-Kosić, Ljubica, Tasić-Otašević, Suzana, Tomanović, Snežana, Kovačević-Filipović, Milica, "Consensus statement on the epidemiological situation and expected frequency of canine vector-borne diseases in Serbia" in Veterinarski glasnik, 74, no. 2 (2020):211-215,
https://doi.org/10.2298/VETGL201124016P . .

TY  - JOUR
AU  - Simin, Verica
AU  - Lalošević, Dušan
AU  - Mijatović, Dragana
AU  - Tomanović, Snežana
AU  - Miljević, Milan
AU  - Čabrilo, Borislav
AU  - Bogdan, Ivana
AU  - Banović, Pavle
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1006
AB  - The primary objectives of this study were (1) to determine the presence of Borrelia burgdorferi infection in ticks removed from patients for the purpose of singling out sites with increased risk of Lyme borreliosis, and (2) to determine the presence of IgM and/or IgG antibodies against B. burgdorferi sensu lato (s. l.) complex in sera of patients who had ticks removed. From 108 ticks removed from patients, all were examined zoologically and a sub-sample of 91 ticks was tested using PCR analysis to determine the presence of DNA indicating B. burgdorferi infection. To detect anti-Borrelia IgM and/ or IgG antibodies in 61 patients bitten by ticks, we used line recombinant immunoblot test. The most common tick identified was Ixodes ricinus. B. burgdorferi s. l. was present in 37 of 91 tested ticks (40.7%). Seroconversion against B. burgdorferi s. l. antigen was detected in 12 of 61 patients (19.7%). Most of the infected ticks were from the province of Vojvodina (11 municipalities), with the city of Novi Sad proving to be the site with the highest number of infected ticks, 6 in total.
AB  - Glavni cilj ove studije je bio da se ispita prisustvo B. burgdorferi infekcije u krpeljma uklonjenih sa pacijenata radi utvđivanja lokaliteta sa povećanim rizikom za obolevanje od lajm borelioze, kao i ispitivanje prisustva IgM i/ili IgG antitela usmerenih protiv B. burgdorferi s. l. kompleksa u serumu pacijenata infestiranih krpeljima. Sa pacijenata je prikupljeno i zoološki ispitano 108 krpelja. PCR analiza krpelja korišc'ena je za utvrđivanje prisustva B. burgdorferi s. l. kompleks kod 91 krpelja od ukupnih 108. Za otkrivanje IgM i/ili IgG antitela protiv B. burgdorferi s. l. korišćeni su linijski rekombinantni imunoblot testovi. Najčešc'i identifikovani krpelj je Ixodes ricinus. B. burgdorferi s. l. je bila prisutna u 37 od 91 testiranog krpelja (40,7%). Prisustvo IgM/IgG antitela protiv B. burgdorferi s. l. otkriveno je kod 12 od 61 pacijenta (19,7%). Najviše inficiarnih krpelja je sa teritorije AP Vojvodine (11 opština), gde je urbani deo Novog Sada lokalitet sa najvećim brojem inficiranih krpelja - 6.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Borellia burgdorferi infection in removed ticks and anti-Borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad
T1  - B. burgdorferi infekcija u krpeljma uklonjenih sa ljudi i prisustvo antitela protiv borelija kod pacijenata infestiranih krpeljima
EP  - 177
IS  - 2
SP  - 164
VL  - 74
DO  - 10.2298/VETGL200527008S
ER  - 

@article{
author = "Simin, Verica and Lalošević, Dušan and Mijatović, Dragana and Tomanović, Snežana and Miljević, Milan and Čabrilo, Borislav and Bogdan, Ivana and Banović, Pavle",
year = "2020",
abstract = "The primary objectives of this study were (1) to determine the presence of Borrelia burgdorferi infection in ticks removed from patients for the purpose of singling out sites with increased risk of Lyme borreliosis, and (2) to determine the presence of IgM and/or IgG antibodies against B. burgdorferi sensu lato (s. l.) complex in sera of patients who had ticks removed. From 108 ticks removed from patients, all were examined zoologically and a sub-sample of 91 ticks was tested using PCR analysis to determine the presence of DNA indicating B. burgdorferi infection. To detect anti-Borrelia IgM and/ or IgG antibodies in 61 patients bitten by ticks, we used line recombinant immunoblot test. The most common tick identified was Ixodes ricinus. B. burgdorferi s. l. was present in 37 of 91 tested ticks (40.7%). Seroconversion against B. burgdorferi s. l. antigen was detected in 12 of 61 patients (19.7%). Most of the infected ticks were from the province of Vojvodina (11 municipalities), with the city of Novi Sad proving to be the site with the highest number of infected ticks, 6 in total., Glavni cilj ove studije je bio da se ispita prisustvo B. burgdorferi infekcije u krpeljma uklonjenih sa pacijenata radi utvđivanja lokaliteta sa povećanim rizikom za obolevanje od lajm borelioze, kao i ispitivanje prisustva IgM i/ili IgG antitela usmerenih protiv B. burgdorferi s. l. kompleksa u serumu pacijenata infestiranih krpeljima. Sa pacijenata je prikupljeno i zoološki ispitano 108 krpelja. PCR analiza krpelja korišc'ena je za utvrđivanje prisustva B. burgdorferi s. l. kompleks kod 91 krpelja od ukupnih 108. Za otkrivanje IgM i/ili IgG antitela protiv B. burgdorferi s. l. korišćeni su linijski rekombinantni imunoblot testovi. Najčešc'i identifikovani krpelj je Ixodes ricinus. B. burgdorferi s. l. je bila prisutna u 37 od 91 testiranog krpelja (40,7%). Prisustvo IgM/IgG antitela protiv B. burgdorferi s. l. otkriveno je kod 12 od 61 pacijenta (19,7%). Najviše inficiarnih krpelja je sa teritorije AP Vojvodine (11 opština), gde je urbani deo Novog Sada lokalitet sa najvećim brojem inficiranih krpelja - 6.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Borellia burgdorferi infection in removed ticks and anti-Borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad, B. burgdorferi infekcija u krpeljma uklonjenih sa ljudi i prisustvo antitela protiv borelija kod pacijenata infestiranih krpeljima",
pages = "177-164",
number = "2",
volume = "74",
doi = "10.2298/VETGL200527008S"
}

Simin, V., Lalošević, D., Mijatović, D., Tomanović, S., Miljević, M., Čabrilo, B., Bogdan, I.,& Banović, P.. (2020). Borellia burgdorferi infection in removed ticks and anti-Borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(2), 164-177.
https://doi.org/10.2298/VETGL200527008S

Simin V, Lalošević D, Mijatović D, Tomanović S, Miljević M, Čabrilo B, Bogdan I, Banović P. Borellia burgdorferi infection in removed ticks and anti-Borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad. in Veterinarski glasnik. 2020;74(2):164-177.
doi:10.2298/VETGL200527008S .

Simin, Verica, Lalošević, Dušan, Mijatović, Dragana, Tomanović, Snežana, Miljević, Milan, Čabrilo, Borislav, Bogdan, Ivana, Banović, Pavle, "Borellia burgdorferi infection in removed ticks and anti-Borrelia antibodies in infested patients admitted to the Pasteur institute, Novi Sad" in Veterinarski glasnik, 74, no. 2 (2020):164-177,
https://doi.org/10.2298/VETGL200527008S . .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Juwaid, Salem
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Radojicić, Sonja
AU  - Hodzić, Adnan
AU  - Duscher, Gerhard Georg
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/929
AB  - Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia
EP  - 324
IS  - 3
SP  - 312
VL  - 69
DO  - 10.2478/acve-2019-0026
ER  - 

@article{
author = "Sukara, Ratko and Juwaid, Salem and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Veinović, Gorana and Radojicić, Sonja and Hodzić, Adnan and Duscher, Gerhard Georg and Tomanović, Snežana",
year = "2019",
abstract = "Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia",
pages = "324-312",
number = "3",
volume = "69",
doi = "10.2478/acve-2019-0026"
}

Sukara, R., Juwaid, S., Ćirović, D., Penezić, A., Mihaljica, D., Veinović, G., Radojicić, S., Hodzić, A., Duscher, G. G.,& Tomanović, S.. (2019). Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3), 312-324.
https://doi.org/10.2478/acve-2019-0026

Sukara R, Juwaid S, Ćirović D, Penezić A, Mihaljica D, Veinović G, Radojicić S, Hodzić A, Duscher GG, Tomanović S. Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd. 2019;69(3):312-324.
doi:10.2478/acve-2019-0026 .

Sukara, Ratko, Juwaid, Salem, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Veinović, Gorana, Radojicić, Sonja, Hodzić, Adnan, Duscher, Gerhard Georg, Tomanović, Snežana, "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia" in Acta Veterinaria-Beograd, 69, no. 3 (2019):312-324,
https://doi.org/10.2478/acve-2019-0026 . .

TY  - JOUR
AU  - Ćakić, Sanja
AU  - Veinović, Gorana
AU  - Cerar, T.
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ruzic-Sabljić, E.
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/934
AB  - Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.
PB  - Wiley, Hoboken
T2  - Medical & Veterinary Entomology
T1  - Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia
EP  - 520
IS  - 4
SP  - 512
VL  - 33
DO  - 10.1111/mve.12392
ER  - 

@article{
author = "Ćakić, Sanja and Veinović, Gorana and Cerar, T. and Mihaljica, Darko and Sukara, Ratko and Ruzic-Sabljić, E. and Tomanović, Snežana",
year = "2019",
abstract = "Spirochetes from the Borrelia burgdorferi sensu lato (s.l.). (Spirochaetales: Spirochaetaceae) species complex, including the causative agents of Lyme borreliosis, have been isolated from ticks, vertebrate reservoirs and humans. Previous analyses based on direct molecular detection in ticks indicated a considerable diversity of B. burgdorferi s.l. complex in Serbia. The present study aimed (a) to isolate borrelia strains from Serbia; (b) to determine their genotypic characteristics; and (c) to establish a collection of viable B. burgdorferi s.l. strains for further biological, ecological and genetic studies. For the present study, 231 adult Ixodes ricinus (Ixodida: Ixodidae) ticks from 16 ecologically different localities in Serbia were individually processed to cultivate B. burgdorferi s.l. This led to the isolation of 36 strains. A hbb gene quantitative real-time polymerase chain reaction (PCR) based on melting temperature determination and ospA gene sequencing were used to genotype the isolated spirochetes. The species identified based on the hbb gene real-time PCR were: Borrelia lusitaniae (44.4%), Borrelia afzelii (36.1%), Borrelia garinii (13.9%) and Borrelia valaisiana (5.6%), whereas the ospA sequence analysis revealed the occurrence of Borrelia bavariensis. This is the first report of the isolation of B. lusitaniae, B. garinii, B. bavariensis and B. valaisiana strains in Serbia.",
publisher = "Wiley, Hoboken",
journal = "Medical & Veterinary Entomology",
title = "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia",
pages = "520-512",
number = "4",
volume = "33",
doi = "10.1111/mve.12392"
}

Ćakić, S., Veinović, G., Cerar, T., Mihaljica, D., Sukara, R., Ruzic-Sabljić, E.,& Tomanović, S.. (2019). Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology
Wiley, Hoboken., 33(4), 512-520.
https://doi.org/10.1111/mve.12392

Ćakić S, Veinović G, Cerar T, Mihaljica D, Sukara R, Ruzic-Sabljić E, Tomanović S. Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia. in Medical & Veterinary Entomology. 2019;33(4):512-520.
doi:10.1111/mve.12392 .

Ćakić, Sanja, Veinović, Gorana, Cerar, T., Mihaljica, Darko, Sukara, Ratko, Ruzic-Sabljić, E., Tomanović, Snežana, "Diversity of Lyme borreliosis spirochetes isolated from ticks in Serbia" in Medical & Veterinary Entomology, 33, no. 4 (2019):512-520,
https://doi.org/10.1111/mve.12392 . .

TY  - JOUR
AU  - Juwaid, Salem
AU  - Sukara, Ratko
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Kavallieratos, Nickolas G.
AU  - Ćirović, Duško
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/940
AB  - Tick-borne haematozoans cause severe diseases in domestic animals, and some of them have zoonotic potential. The results of previous studies in Europe point to the important role of foxes in natural endemic cycles of several tick-borne pathogens, including protozoa. The aim of the present research was to acquire information on the prevalence and distribution of tick-borne protozoan parasites among foxes in Serbia. Legally hunted foxes from 14 localities throughout Serbia were analysed. Spleen samples were collected from 129 animals and tested for the presence of Babesia spp. and Hepatozoon spp. by PCR. In total, 79/129 (61.2%) of the tested foxes were positive for H. canis, while the presence of two Babesia species was confirmed: B. vulpes (37/129, 28.7%) and B. canis (1/129, 0.8%). Co-infection with B. vulpes and H. canis was present in 26/129 (20.2%) foxes and one animal (1/129, 0.8%) was co-infected by B. canis and H. canis. The results of this study indicate the important role of foxes in the epizootiology of B. vulpes and H. canis in the Republic of Serbia and stress the need for further research to clarify all elements of the enzootic cycle of the detected pathogens, including other reservoirs, vectors, and transmission routes.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia
EP  - 80
IS  - 1
SP  - 70
VL  - 67
DO  - 10.1556/004.2019.008
ER  - 

@article{
author = "Juwaid, Salem and Sukara, Ratko and Penezić, Aleksandra and Mihaljica, Darko and Veinović, Gorana and Kavallieratos, Nickolas G. and Ćirović, Duško and Tomanović, Snežana",
year = "2019",
abstract = "Tick-borne haematozoans cause severe diseases in domestic animals, and some of them have zoonotic potential. The results of previous studies in Europe point to the important role of foxes in natural endemic cycles of several tick-borne pathogens, including protozoa. The aim of the present research was to acquire information on the prevalence and distribution of tick-borne protozoan parasites among foxes in Serbia. Legally hunted foxes from 14 localities throughout Serbia were analysed. Spleen samples were collected from 129 animals and tested for the presence of Babesia spp. and Hepatozoon spp. by PCR. In total, 79/129 (61.2%) of the tested foxes were positive for H. canis, while the presence of two Babesia species was confirmed: B. vulpes (37/129, 28.7%) and B. canis (1/129, 0.8%). Co-infection with B. vulpes and H. canis was present in 26/129 (20.2%) foxes and one animal (1/129, 0.8%) was co-infected by B. canis and H. canis. The results of this study indicate the important role of foxes in the epizootiology of B. vulpes and H. canis in the Republic of Serbia and stress the need for further research to clarify all elements of the enzootic cycle of the detected pathogens, including other reservoirs, vectors, and transmission routes.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia",
pages = "80-70",
number = "1",
volume = "67",
doi = "10.1556/004.2019.008"
}

Juwaid, S., Sukara, R., Penezić, A., Mihaljica, D., Veinović, G., Kavallieratos, N. G., Ćirović, D.,& Tomanović, S.. (2019). First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 67(1), 70-80.
https://doi.org/10.1556/004.2019.008

Juwaid S, Sukara R, Penezić A, Mihaljica D, Veinović G, Kavallieratos NG, Ćirović D, Tomanović S. First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia. in Acta Veterinaria Hungarica. 2019;67(1):70-80.
doi:10.1556/004.2019.008 .

Juwaid, Salem, Sukara, Ratko, Penezić, Aleksandra, Mihaljica, Darko, Veinović, Gorana, Kavallieratos, Nickolas G., Ćirović, Duško, Tomanović, Snežana, "First evidence of tick-borne protozoan pathogens, babesia sp. And hepatozoon canis, in red foxes (vulpes vulpes) in Serbia" in Acta Veterinaria Hungarica, 67, no. 1 (2019):70-80,
https://doi.org/10.1556/004.2019.008 . .

TY  - CONF
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Sukara, Ratko
AU  - Ćakić, Sanja
AU  - Tomanović, Snežana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1411
PB  - Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)
C3  - International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts
T1  - Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia
EP  - 112
SP  - P77(112)
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1411
ER  - 

@conference{
author = "Veinović, Gorana and Mihaljica, Darko and Sukara, Ratko and Ćakić, Sanja and Tomanović, Snežana",
year = "2019",
publisher = "Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)",
journal = "International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts",
title = "Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia",
pages = "112-P77(112)",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1411"
}

Veinović, G., Mihaljica, D., Sukara, R., Ćakić, S.,& Tomanović, S.. (2019). Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia. in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts
Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)., P77(112)-112.
https://hdl.handle.net/21.15107/rcub_rimi_1411

Veinović G, Mihaljica D, Sukara R, Ćakić S, Tomanović S. Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia. in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts. 2019;:P77(112)-112.
https://hdl.handle.net/21.15107/rcub_rimi_1411 .

Veinović, Gorana, Mihaljica, Darko, Sukara, Ratko, Ćakić, Sanja, Tomanović, Snežana, "Complement-mediated serum susceptibility of Borrelia burgdorferi sensu lato strains from Serbia" in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts (2019):P77(112)-112,
https://hdl.handle.net/21.15107/rcub_rimi_1411 .

TY  - CONF
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Juwaid, Salem
AU  - Veinović, Gorana
AU  - Mihaljica, Darko
AU  - Penezić, Aleksandra
AU  - Ćirović, Duško
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1412
PB  - Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)
C3  - International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts
T1  - Lyme borreliosis spirochaetes in red foxes (Vulpes vulpes) from Serbia
SP  - P83
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1412
ER  - 

@conference{
author = "Tomanović, Snežana and Sukara, Ratko and Juwaid, Salem and Veinović, Gorana and Mihaljica, Darko and Penezić, Aleksandra and Ćirović, Duško",
year = "2019",
publisher = "Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)",
journal = "International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts",
title = "Lyme borreliosis spirochaetes in red foxes (Vulpes vulpes) from Serbia",
pages = "P83",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1412"
}

Tomanović, S., Sukara, R., Juwaid, S., Veinović, G., Mihaljica, D., Penezić, A.,& Ćirović, D.. (2019). Lyme borreliosis spirochaetes in red foxes (Vulpes vulpes) from Serbia. in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts
Austrian Society of Hygiene and Preventive Medicine (ÖGHMP), ESCMID Study Group for Lyme Borreliosis (ESGBOR)., P83.
https://hdl.handle.net/21.15107/rcub_rimi_1412

Tomanović S, Sukara R, Juwaid S, Veinović G, Mihaljica D, Penezić A, Ćirović D. Lyme borreliosis spirochaetes in red foxes (Vulpes vulpes) from Serbia. in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts. 2019;:P83.
https://hdl.handle.net/21.15107/rcub_rimi_1412 .

Tomanović, Snežana, Sukara, Ratko, Juwaid, Salem, Veinović, Gorana, Mihaljica, Darko, Penezić, Aleksandra, Ćirović, Duško, "Lyme borreliosis spirochaetes in red foxes (Vulpes vulpes) from Serbia" in International Symposium on Tick-Borne Pathogens and Disease, ITPD 2019, 8-11th September 2019, Vienna, Austria - Book of Abstracts (2019):P83,
https://hdl.handle.net/21.15107/rcub_rimi_1412 .