TY  - JOUR
AU  - Simin, Stanislav
AU  - Tomanović, Snežana
AU  - Sukara, Ratko
AU  - Stefanov, Marijana
AU  - Savović, Milan
AU  - Gajić, Bojan
AU  - Lalošević, Vesna
PY  - 2024
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1477
AB  - The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Microorganisms
T1  - Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans
IS  - 2
SP  - 233
VL  - 12
DO  - 10.3390/microorganisms12020233
ER  - 

@article{
author = "Simin, Stanislav and Tomanović, Snežana and Sukara, Ratko and Stefanov, Marijana and Savović, Milan and Gajić, Bojan and Lalošević, Vesna",
year = "2024",
abstract = "The “beautiful viviparous fly”, Wohlfahrtia magnifica, may have a magnificent appearance due to its striking morphology; however, it is a potentially deadly agent of obligate traumatic myiasis in humans and animals, with a serious impact on welfare and economics. The fly is found across the Palearctic realm, including the Western Balkan region, with reports from former Yugoslavian countries from the first half of the 20th century. In this paper, a recent case of wohlfahrtiosis recorded in Northern Serbia is evidenced using morphological and molecular techniques. Larvae were collected from two adult sheep with severe hoof myiasis and two young sheep with genital and interdigital myiasis. Morphological identification was performed for adults bred from the infested vulva and third-stage larvae (L3) collected from the hoof wounds, supported with barcoding sequences of the COI gene obtained from larval pairs from the hoof wounds of older and the genitalia of younger sheep. W. magnifica was identified according to the appearance of male fly terminalia and the morphology of L3, which was confirmed after the comparison of representative sequences of the COI gene (deposited in GenBank™ under accession numbers MT027108–MT027114) to those available in GenBank™. This finding represents the first reported case of wohlfahrtiosis in the Western Balkans in 80 years, highlighting the need to re-inform relevant stakeholders to achieve adequate disease control.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Microorganisms",
title = "Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans",
number = "2",
pages = "233",
volume = "12",
doi = "10.3390/microorganisms12020233"
}

Simin, S., Tomanović, S., Sukara, R., Stefanov, M., Savović, M., Gajić, B.,& Lalošević, V.. (2024). Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans. in Microorganisms
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 233.
https://doi.org/10.3390/microorganisms12020233

Simin S, Tomanović S, Sukara R, Stefanov M, Savović M, Gajić B, Lalošević V. Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans. in Microorganisms. 2024;12(2):233.
doi:10.3390/microorganisms12020233 .

Simin, Stanislav, Tomanović, Snežana, Sukara, Ratko, Stefanov, Marijana, Savović, Milan, Gajić, Bojan, Lalošević, Vesna, "Long Time No Hear, Magnificent Wohlfahrtia! Morphological and Molecular Evidence of Almost Forgotten Flesh Fly in Serbia and Western Balkans" in Microorganisms, 12, no. 2 (2024):233,
https://doi.org/10.3390/microorganisms12020233 . .