TY  - JOUR
AU  - Tomanović, Snežana
AU  - Chochlakis, Dimosthenis
AU  - Radulović, Željko
AU  - Milutinović, Marija
AU  - Ćakić, Sanja
AU  - Mihaljica, Darko
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
PY  - 2013
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/516
AB  - Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.
PB  - Springer, Dordrecht
T2  - Experimental & Applied Acarology
T1  - Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia
EP  - 376
IS  - 3
SP  - 367
VL  - 59
DO  - 10.1007/s10493-012-9597-y
ER  - 

@article{
author = "Tomanović, Snežana and Chochlakis, Dimosthenis and Radulović, Željko and Milutinović, Marija and Ćakić, Sanja and Mihaljica, Darko and Tselentis, Yannis and Psaroulaki, Anna",
year = "2013",
abstract = "Past studies in Serbia have reported concurrent infections of Ixodes ricinus ticks with Borrelia burgdorferi sensu lato genospecies, Anaplasma phagocytophilum and Francisella tularensis. As a step forward, this investigation included a broader range of microorganisms and five most common and abundant tick species in Serbia. Five tick species were identified (Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, H. concinna and I. ricinus) and analyzed for the presence of seven pathogens. Anaplasma ovis, A. phagocytophilum, Babesia canis, B. burgdorferi s.l., Coxiella burnetii, Rickettsia helvetica and R. monacensis were detected. Sequencing of samples positive for F. tularensis revealed the presence of Francisella-like endosymbionts. No Bartonella spp. DNA was amplified. Concurrent infections were present in three tick species (D. reticulatus, H. concinna and I. ricinus). The rate of co-infections was highest in I. ricinus (20/27), while this tick species harbored the broadest range of co-infection combinations, with dual, triple and a quadruple infection(s) being detected.",
publisher = "Springer, Dordrecht",
journal = "Experimental & Applied Acarology",
title = "Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia",
pages = "376-367",
number = "3",
volume = "59",
doi = "10.1007/s10493-012-9597-y"
}

Tomanović, S., Chochlakis, D., Radulović, Ž., Milutinović, M., Ćakić, S., Mihaljica, D., Tselentis, Y.,& Psaroulaki, A.. (2013). Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia. in Experimental & Applied Acarology
Springer, Dordrecht., 59(3), 367-376.
https://doi.org/10.1007/s10493-012-9597-y

Tomanović S, Chochlakis D, Radulović Ž, Milutinović M, Ćakić S, Mihaljica D, Tselentis Y, Psaroulaki A. Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia. in Experimental & Applied Acarology. 2013;59(3):367-376.
doi:10.1007/s10493-012-9597-y .

Tomanović, Snežana, Chochlakis, Dimosthenis, Radulović, Željko, Milutinović, Marija, Ćakić, Sanja, Mihaljica, Darko, Tselentis, Yannis, Psaroulaki, Anna, "Analysis of pathogen co-occurrence in host-seeking adult hard ticks from Serbia" in Experimental & Applied Acarology, 59, no. 3 (2013):367-376,
https://doi.org/10.1007/s10493-012-9597-y . .

TY  - JOUR
AU  - Radulović, Željko
AU  - Chochlakis, Dimosthenis
AU  - Tomanović, Snežana
AU  - Milutinović, Marija
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
PY  - 2011
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/361
AB  - Ticks can transmit multiple pathogenic bacteria responsible for diseases in animals and humans such as Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and spotted fever group Rickettsia sp. The current study aimed to investigate the presence of Rickettsiae in ticks collected from seven localities in Serbia. One hundred thirty-one (131) questing ticks belonging to 5 tick species (Dermacentor marginatus, Dermacentor reticulatus, Haemaphysalis punctata, Haemaphysalis Concinna, and Ixodes ricinus) were collected in 2007 and 2009. Ticks were tested by polymerase chain reaction, amplifying gltA, ompA, and 17-kd genes, and sequencing analysis, revealing the presence of Rickettsia helvetica and Rickettsia monacensis in I. ricinus ticks only (infection rates 7.7% and 15.4% for R. helvetica and R. monacensis, respectively). R. helvetica has been isolated from I. ricinus ticks and has been implicated in fatal perimyocarditis. R. monacensis was first identified in I. ricinus samples collected in Germany and has recently been implicated in human infection. The results of the current study make fundamental the need to evaluate the incidence of infection with R. helvetica and R. monacensis among the resident population.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Vector-Borne & Zoonotic Diseases
T1  - First Detection of Spotted Fever Group Rickettsiae in Ticks in Serbia
EP  - 115
IS  - 2
SP  - 111
VL  - 11
DO  - 10.1089/vbz.2009.0254
ER  - 

@article{
author = "Radulović, Željko and Chochlakis, Dimosthenis and Tomanović, Snežana and Milutinović, Marija and Tselentis, Yannis and Psaroulaki, Anna",
year = "2011",
abstract = "Ticks can transmit multiple pathogenic bacteria responsible for diseases in animals and humans such as Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and spotted fever group Rickettsia sp. The current study aimed to investigate the presence of Rickettsiae in ticks collected from seven localities in Serbia. One hundred thirty-one (131) questing ticks belonging to 5 tick species (Dermacentor marginatus, Dermacentor reticulatus, Haemaphysalis punctata, Haemaphysalis Concinna, and Ixodes ricinus) were collected in 2007 and 2009. Ticks were tested by polymerase chain reaction, amplifying gltA, ompA, and 17-kd genes, and sequencing analysis, revealing the presence of Rickettsia helvetica and Rickettsia monacensis in I. ricinus ticks only (infection rates 7.7% and 15.4% for R. helvetica and R. monacensis, respectively). R. helvetica has been isolated from I. ricinus ticks and has been implicated in fatal perimyocarditis. R. monacensis was first identified in I. ricinus samples collected in Germany and has recently been implicated in human infection. The results of the current study make fundamental the need to evaluate the incidence of infection with R. helvetica and R. monacensis among the resident population.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Vector-Borne & Zoonotic Diseases",
title = "First Detection of Spotted Fever Group Rickettsiae in Ticks in Serbia",
pages = "115-111",
number = "2",
volume = "11",
doi = "10.1089/vbz.2009.0254"
}

Radulović, Ž., Chochlakis, D., Tomanović, S., Milutinović, M., Tselentis, Y.,& Psaroulaki, A.. (2011). First Detection of Spotted Fever Group Rickettsiae in Ticks in Serbia. in Vector-Borne & Zoonotic Diseases
Mary Ann Liebert, Inc, New Rochelle., 11(2), 111-115.
https://doi.org/10.1089/vbz.2009.0254

Radulović Ž, Chochlakis D, Tomanović S, Milutinović M, Tselentis Y, Psaroulaki A. First Detection of Spotted Fever Group Rickettsiae in Ticks in Serbia. in Vector-Borne & Zoonotic Diseases. 2011;11(2):111-115.
doi:10.1089/vbz.2009.0254 .

Radulović, Željko, Chochlakis, Dimosthenis, Tomanović, Snežana, Milutinović, Marija, Tselentis, Yannis, Psaroulaki, Anna, "First Detection of Spotted Fever Group Rickettsiae in Ticks in Serbia" in Vector-Borne & Zoonotic Diseases, 11, no. 2 (2011):111-115,
https://doi.org/10.1089/vbz.2009.0254 . .

TY  - JOUR
AU  - Radulović, Željko
AU  - Milutinović, Marija
AU  - Tomanović, Snežana
AU  - Mulenga, Albert
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/274
AB  - We have previously found apparent differences in Gpdh allele frequences between borrelia infected and uninfected Ixodes riotous as revealed by native gel electrophoresis of allozyme polymorphisms. The present study deals with the genetic basis of the observed allozyme polymorphism. Multiple sequence alignment of 36 Gpdh open reading frames identified a total of 40 polymorphic nucleotide sites. Of the 40 polymorphic nucleotide sites, 34 were silent (did not result in amino acid residue change), while six were active causing a change in the amino acid chain. All polymorphic amino acid sites were situated within the N-terminal NAD-binding domain, whereas the C-terminal substrate-binding domain was highly conserved. Analysis of the obtained Gpdh sequences and GPDH allozyme polymorphisms for individual ticks pointed to amino acid changes at positions 61 (glycine-to-glutamic acid), 64 (serine-to-cysteine) and 102 (glycine-to-arginine) as a key for differential mobility of GPDH allozymes in an electric field. Our findings are discussed in the context of the molecular basis of I. ricinus host finding behavior.
PB  - EDP Sciences S A, Les Ulis Cedex A
T2  - Parasite
T1  - Exon variability of gene encoding glycerol-3-phosphate dehydrogenase of ixodes ricinus ticks
EP  - 368
IS  - 4
SP  - 363
VL  - 17
DO  - 10.1051/parasite/2010174363
ER  - 

@article{
author = "Radulović, Željko and Milutinović, Marija and Tomanović, Snežana and Mulenga, Albert",
year = "2010",
abstract = "We have previously found apparent differences in Gpdh allele frequences between borrelia infected and uninfected Ixodes riotous as revealed by native gel electrophoresis of allozyme polymorphisms. The present study deals with the genetic basis of the observed allozyme polymorphism. Multiple sequence alignment of 36 Gpdh open reading frames identified a total of 40 polymorphic nucleotide sites. Of the 40 polymorphic nucleotide sites, 34 were silent (did not result in amino acid residue change), while six were active causing a change in the amino acid chain. All polymorphic amino acid sites were situated within the N-terminal NAD-binding domain, whereas the C-terminal substrate-binding domain was highly conserved. Analysis of the obtained Gpdh sequences and GPDH allozyme polymorphisms for individual ticks pointed to amino acid changes at positions 61 (glycine-to-glutamic acid), 64 (serine-to-cysteine) and 102 (glycine-to-arginine) as a key for differential mobility of GPDH allozymes in an electric field. Our findings are discussed in the context of the molecular basis of I. ricinus host finding behavior.",
publisher = "EDP Sciences S A, Les Ulis Cedex A",
journal = "Parasite",
title = "Exon variability of gene encoding glycerol-3-phosphate dehydrogenase of ixodes ricinus ticks",
pages = "368-363",
number = "4",
volume = "17",
doi = "10.1051/parasite/2010174363"
}

Radulović, Ž., Milutinović, M., Tomanović, S.,& Mulenga, A.. (2010). Exon variability of gene encoding glycerol-3-phosphate dehydrogenase of ixodes ricinus ticks. in Parasite
EDP Sciences S A, Les Ulis Cedex A., 17(4), 363-368.
https://doi.org/10.1051/parasite/2010174363

Radulović Ž, Milutinović M, Tomanović S, Mulenga A. Exon variability of gene encoding glycerol-3-phosphate dehydrogenase of ixodes ricinus ticks. in Parasite. 2010;17(4):363-368.
doi:10.1051/parasite/2010174363 .

Radulović, Željko, Milutinović, Marija, Tomanović, Snežana, Mulenga, Albert, "Exon variability of gene encoding glycerol-3-phosphate dehydrogenase of ixodes ricinus ticks" in Parasite, 17, no. 4 (2010):363-368,
https://doi.org/10.1051/parasite/2010174363 . .

TY  - JOUR
AU  - Tomanović, Snežana
AU  - Radulović, Željko
AU  - Masuzawa, Toshiyuki
AU  - Milutinović, Marija
PY  - 2010
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/271
AB  - The list of tick-borne pathogens is long, varied and includes viruses, bacteria, protozoa and nematodes. As all of these agents can exist in ticks, their co-infections hove been previously reported. We studied co-infections of emerging bacterial pathogens (Borrelia burgdorferi sensu lato, Ana plasma phagocytophilum and Francisella tularensis) in Ixodes ricinus ticks in Serbia. Using PCR technique, we detected species-specific sequences, rrf-rrl rDNA intergenic spacer for B. burgdorferi s.l., p44/msp2 paralogs for A. phagocytophilum, and the 17 kDa lipoprotein gene, TUL4, for F tularensis, respectively, in total DNA extracted from the ticks. Common infections with more than one pathogen were detected in 42 (28.8 %) of 146 infected l. ricinus ticks. Co-infections with two pathogens were present in 39 (26.7 %) of infected ticks. Simultaneous presence of A. phagocytophilum and different genospecies of B. burgdorferi s.l. complex was recorded in 16 ticks, co-infection with different B. burgdorferi s. l. genospecies was found in 15 ticks and eight ticks harbored mixed infections with F tularensis and B. burgdorferi s.l. genospecies. Less common were triple pathogen species infections, detected in three ticks, one infected with A. phagocytophilum / B. burgdorferi s.s. / B. lusitaniae and two infected with F tularensis / B. burgdorferi s.s. / B. lusitaniae. No mixed infections of A. phagocytophilum and F. tularensis were detected.
PB  - EDP Sciences S A, Les Ulis Cedex A
T2  - Parasite
T1  - Coexistence of emerging bacterial pathogens in ixodes ricinus ticks in Serbia
EP  - 217
IS  - 3
SP  - 211
VL  - 17
DO  - 10.1051/parasite/2010173211
ER  - 

@article{
author = "Tomanović, Snežana and Radulović, Željko and Masuzawa, Toshiyuki and Milutinović, Marija",
year = "2010",
abstract = "The list of tick-borne pathogens is long, varied and includes viruses, bacteria, protozoa and nematodes. As all of these agents can exist in ticks, their co-infections hove been previously reported. We studied co-infections of emerging bacterial pathogens (Borrelia burgdorferi sensu lato, Ana plasma phagocytophilum and Francisella tularensis) in Ixodes ricinus ticks in Serbia. Using PCR technique, we detected species-specific sequences, rrf-rrl rDNA intergenic spacer for B. burgdorferi s.l., p44/msp2 paralogs for A. phagocytophilum, and the 17 kDa lipoprotein gene, TUL4, for F tularensis, respectively, in total DNA extracted from the ticks. Common infections with more than one pathogen were detected in 42 (28.8 %) of 146 infected l. ricinus ticks. Co-infections with two pathogens were present in 39 (26.7 %) of infected ticks. Simultaneous presence of A. phagocytophilum and different genospecies of B. burgdorferi s.l. complex was recorded in 16 ticks, co-infection with different B. burgdorferi s. l. genospecies was found in 15 ticks and eight ticks harbored mixed infections with F tularensis and B. burgdorferi s.l. genospecies. Less common were triple pathogen species infections, detected in three ticks, one infected with A. phagocytophilum / B. burgdorferi s.s. / B. lusitaniae and two infected with F tularensis / B. burgdorferi s.s. / B. lusitaniae. No mixed infections of A. phagocytophilum and F. tularensis were detected.",
publisher = "EDP Sciences S A, Les Ulis Cedex A",
journal = "Parasite",
title = "Coexistence of emerging bacterial pathogens in ixodes ricinus ticks in Serbia",
pages = "217-211",
number = "3",
volume = "17",
doi = "10.1051/parasite/2010173211"
}

Tomanović, S., Radulović, Ž., Masuzawa, T.,& Milutinović, M.. (2010). Coexistence of emerging bacterial pathogens in ixodes ricinus ticks in Serbia. in Parasite
EDP Sciences S A, Les Ulis Cedex A., 17(3), 211-217.
https://doi.org/10.1051/parasite/2010173211

Tomanović S, Radulović Ž, Masuzawa T, Milutinović M. Coexistence of emerging bacterial pathogens in ixodes ricinus ticks in Serbia. in Parasite. 2010;17(3):211-217.
doi:10.1051/parasite/2010173211 .

Tomanović, Snežana, Radulović, Željko, Masuzawa, Toshiyuki, Milutinović, Marija, "Coexistence of emerging bacterial pathogens in ixodes ricinus ticks in Serbia" in Parasite, 17, no. 3 (2010):211-217,
https://doi.org/10.1051/parasite/2010173211 . .