Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration
2015
Аутори
Krstić, JelenaObradović, Hristina
Jauković, Aleksandra
Okić Đorđević, Ivana
Trivanović, Drenka
Kukolj, Tamara
Mojsilović, Slavko
Ilić, Vesna
Santibanez, Juan F.
Bugarski, Diana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB...-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.
Кључне речи:
Interleukin-17 / Peripheral blood mesenchymal stem cells / Urokinase type plasminogen activator / Migration / Transendothelial migrationИзвор:
Biochimica et Biophysica Acta-Molecular Cell Research, 2015, 1853, 2, 431-444Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Регенеративни и модулаторни потенцијал адултних матичних ћелија (RS-MESTD-Basic Research (BR or ON)-175062)
DOI: 10.1016/j.bbamcr.2014.11.025
ISSN: 0167-4889
PubMed: 25433194
WoS: 000348554600017
Scopus: 2-s2.0-84916910734
Институција/група
Institut za medicinska istraživanjaTY - JOUR AU - Krstić, Jelena AU - Obradović, Hristina AU - Jauković, Aleksandra AU - Okić Đorđević, Ivana AU - Trivanović, Drenka AU - Kukolj, Tamara AU - Mojsilović, Slavko AU - Ilić, Vesna AU - Santibanez, Juan F. AU - Bugarski, Diana PY - 2015 UR - http://rimi.imi.bg.ac.rs/handle/123456789/666 AB - Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development. PB - Elsevier Science Bv, Amsterdam T2 - Biochimica et Biophysica Acta-Molecular Cell Research T1 - Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration EP - 444 IS - 2 SP - 431 VL - 1853 DO - 10.1016/j.bbamcr.2014.11.025 ER -
@article{ author = "Krstić, Jelena and Obradović, Hristina and Jauković, Aleksandra and Okić Đorđević, Ivana and Trivanović, Drenka and Kukolj, Tamara and Mojsilović, Slavko and Ilić, Vesna and Santibanez, Juan F. and Bugarski, Diana", year = "2015", abstract = "Mesenchymal stem cells (MSCs) have the potential to migrate toward damaged tissues increasing tissue regeneration. Interleukin-17 (IL-17) is a proinflammatory cytokine with pleiotropic effects associated with many inflammatory diseases. Although IL-17 can modulate MSC functions, its capacity to regulate MSC migration is not well elucidated so far. Here, we studied the role of IL-17 on peripheral blood (PB) derived MSC migration and transmigration across endothelial cells. IL-17 increased PB-MSC migration in a wound healing assay as well as cell mobilization from collagen gel. Concomitantly IL-17 induced the expression of urokinase type plasminogen activator (uPA) without affecting matrix metalloproteinase expression. The incremented uPA expression mediated the capacity of IL-17 to enhance PB-MSC migration in a ERK1,2 MAPK dependent way. Also, IL-17 induced PB-MSC migration alongside with changes in cell polarization and uPA localization in cell protrusions. Moreover, IL-17 increased PB-MSC adhesion to endothelial cells and transendothelial migration, as well as increased the capacity of PB-MSC adhesion to fibronectin, in an uPA-dependent fashion. Therefore, our data suggested that IL-17 may act as chemotropic factor for PB-MSCs by incrementing cell motility and uPA expression during inflammation development.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Biochimica et Biophysica Acta-Molecular Cell Research", title = "Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration", pages = "444-431", number = "2", volume = "1853", doi = "10.1016/j.bbamcr.2014.11.025" }
Krstić, J., Obradović, H., Jauković, A., Okić Đorđević, I., Trivanović, D., Kukolj, T., Mojsilović, S., Ilić, V., Santibanez, J. F.,& Bugarski, D.. (2015). Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration. in Biochimica et Biophysica Acta-Molecular Cell Research Elsevier Science Bv, Amsterdam., 1853(2), 431-444. https://doi.org/10.1016/j.bbamcr.2014.11.025
Krstić J, Obradović H, Jauković A, Okić Đorđević I, Trivanović D, Kukolj T, Mojsilović S, Ilić V, Santibanez JF, Bugarski D. Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration. in Biochimica et Biophysica Acta-Molecular Cell Research. 2015;1853(2):431-444. doi:10.1016/j.bbamcr.2014.11.025 .
Krstić, Jelena, Obradović, Hristina, Jauković, Aleksandra, Okić Đorđević, Ivana, Trivanović, Drenka, Kukolj, Tamara, Mojsilović, Slavko, Ilić, Vesna, Santibanez, Juan F., Bugarski, Diana, "Urokinase type plasminogen activator mediates Interleukin-17-induced peripheral blood mesenchymal stem cell motility and transendothelial migration" in Biochimica et Biophysica Acta-Molecular Cell Research, 1853, no. 2 (2015):431-444, https://doi.org/10.1016/j.bbamcr.2014.11.025 . .