TY  - CONF
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Životić, Maja
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1377
AB  - Prethodne studije su pokazale da klotho poseduje anti-inflamatornu, antifibrotičku i antioksidativnu ulogu, i da postoji direktna pozitivna korelacija izmedu klotho-a i albuminurije kod nekih animalnih modela hronične bubrežne bolesti (CKD). Fokalno segmentna glomeruloskleroza (FSGS) je oblik CKD, koji se odlikuje prisutnom proteinurijom, hipoalbuminemijom i oksidativnim stresom. Ranije smo pokazali da blokada receptora angiotenzina II tipa 1 losartanom (L) smanjuje proteinuriju, glomerulosklerozu, tubulointersticijsku inflamaciju i fibrozu u animalnom modelu FSGS izazvane adriamicinom (ADR, antitumorski lek) kod spontano hipertenzivnih pacova (SHR), ali ove promene u bubrezima su i dalje bile veće od kontrolnih vrednosti. Ekstrakt lista masline (Olea europaea L.) (OLE), dobro poznat kao prirodni antioksidans, bogat je fenolnim jedinjenjima koja se smatraju odgovornim za snažna antioksidativna i anti-inflamatorna dejstva ekstrakta. Stoga smo ispitivali da li tretman losartanom u kombinaciji sa ekstraktom lista masline može biti efikasniji u usporavanju progresije FSGS. Odrasle ženke SHR su podeljene u četiri grupe. Kontrolni pacovi su primili fiziološki rastvor. SHADR, SHADR+L i SHADR+L+OLE grupe su primile ADR (2 mg/kg telesne mase i.v.) dva puta u intervalu od 3 nedelje. Nakon druge injekcije, SHADR+L i SHADR+L+OLE grupe su primale gavažom L (10 mg/kg/dan), odnosno L+OLE (10+80 mg/kg/dan). Na kraju 6-nedeljnog tretmana analizirali smo albuminuriju, nivo azot monoksida (NO,), 6-nitrotriptofan (marker nitracije proteina), klotho i histopatologiju bubrega. U model grupi uočeno je mačajno povećanje albuminurije i indeksa oitećenja bubrega, ito je bilo praćeno značajno smanjenom ekspresijom klotho proteina i 6-nitortriptofana, kao i nivoa NO, u homogenatu bubrega u poredenju sa ovim vrednostima u kontrolnoj grupi. Tretman losartanom je mačajno smanjio albuminuriju, glomerulosklerozu i tubulointersticijska oštećenja u poredenju sa model grupom, ali su oštećenja bubrega i dalje bila mačajno veća nego u kontrolnoj grupi. Takode, vrednost NO, je vraćena na kontrolni nivo, a proteinska ekspresija 6-nitrotriptofana je mačajno povećana, dok je klotho-a ostala nepromenjena, u poredenju sa model grupom. Interesantno, kada je tretmanu losartanom dodat ekstrakt lista masline primećeno je mačajno i još jače smanjenje albuminurije, glomeruloskleroze, tubulointersticijske inflamacije i fibroze u poredenju sa model grupom. Takode, ovi parametri su sniženi do nivoa koji se nisu mačajno razlikovali od onih u kontroli. Pored toga, ekspresija proteina klotho-a u bubrezima je mačajno povećana u poredenju sa grupama SHADR+L i model Naši rezultati su pokazali da je ekstrakt lista masline kombinovan sa losartanom bio efikasniji od samog tretmana losartanom u usporavanju progresije FSGS kod SHR, a ovaj povoljan efekat uključivao je Klotho signalni put.
AB  - Previous studies have shown the anti-inflammatory, antifibrotic and antioxidant properties of klotho, and a direct positive correlation between klotho and albuminuria in some chronic kidney disease (CKD) animal models. Focal segmented glomerulosclerosis (FSGS), a form of CKD, is distinguished by proteinuria, hypoalbuminemia, and oxidative stress. We previously showed that blockade of angiotensin Õ type 1 receptor with losartan (L) decreased proteinuria, glomerulosclerosis, tubulointerstitial inflammation, and fibrosis in animal model of FSGS induced by Adriamycin (ADR, anti-cancer agent) in spontaneously hypertensive rats (SHR), but these renal alterations were still higher than control values. Olive (Olea europaea L.) leaf extract (OLE), well-known as natural antioxidant, is rich in phenolic compounds that are thought to be responsible for its powerful antioxidant and anti-inflammatory properties. Here, we examined if losartan treatment in combination with olive leaf extract consumption could be more effective in slowing down the progression of FSGS. Adult female SHRs were divided in four groups. Control rats received vehicle. SHADR, SHADR+L and SHADR+L+OLE groups received ADR (2 mg/kg body weight i.v.) twice in 3-week-interval. After the second injection, SHADR+L, and SHADR+L+OLE groups received by gavage L (10 mg/kg/day), and L+OLE (10+80 mg/kg/day), respectively. At the end of6-week treatment, we analysed albuminuria, renal nitric oxide level (NO,), 6-nitrotryptophan (marker ofnitration of proteins), klotho, and renal histopathology In model group we found significantly increased albuininuria and renal injury score, followed by significantly decreased klotho protein expressions, NOx level, and 6- nitortryptophan in comparison to these values in control group. Losartan treatment significantly reduced albuminuria, glomerulosclerosis, and tubulointerstitial injuries compared to model group, but renal alterations were still significantly higher than in control group. Furthermore, NO, level reversed to control and 6-nitrotryptophan protein expression significantly increased, but klotho remained unchanged, compared to model group. Interestingly, when olive leaf extract was added to losartan treatment, a significant and even stronger decrease of albuminuria, glomerulosclerosis, tubulointerstitial inflammation and fibrosis were observed in comparison to model group. Also, these parameters were lowered to the levels not significantly different than in control. Furthermore, renal protein expression of klotho was significantly increased compared to SHADR+L and model groups. Our results showed that olive leaf extract in addition to losartan treatment was more effective than losartan alone in slowing down the progression of FSGS in SHR, and that beneficial effect involved the klotho signalling pathway.
PB  - Niš: Punta
C3  - Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book
T1  - Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho
T1  - Ekstrakt lista masline u kombinaciji sa Losartanom ima pozitivan efekat na albuminuriju i progresivnu fokalnu segmentalnu glomerulosklerozu u hipertenzivnih miševa: uloga Klotho
EP  - 159
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1377
ER  - 

@conference{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Grujić-Milanović, Jelica and Životić, Maja and Dekanski, Dragana and Jovović, Đurđica",
year = "2023",
abstract = "Prethodne studije su pokazale da klotho poseduje anti-inflamatornu, antifibrotičku i antioksidativnu ulogu, i da postoji direktna pozitivna korelacija izmedu klotho-a i albuminurije kod nekih animalnih modela hronične bubrežne bolesti (CKD). Fokalno segmentna glomeruloskleroza (FSGS) je oblik CKD, koji se odlikuje prisutnom proteinurijom, hipoalbuminemijom i oksidativnim stresom. Ranije smo pokazali da blokada receptora angiotenzina II tipa 1 losartanom (L) smanjuje proteinuriju, glomerulosklerozu, tubulointersticijsku inflamaciju i fibrozu u animalnom modelu FSGS izazvane adriamicinom (ADR, antitumorski lek) kod spontano hipertenzivnih pacova (SHR), ali ove promene u bubrezima su i dalje bile veće od kontrolnih vrednosti. Ekstrakt lista masline (Olea europaea L.) (OLE), dobro poznat kao prirodni antioksidans, bogat je fenolnim jedinjenjima koja se smatraju odgovornim za snažna antioksidativna i anti-inflamatorna dejstva ekstrakta. Stoga smo ispitivali da li tretman losartanom u kombinaciji sa ekstraktom lista masline može biti efikasniji u usporavanju progresije FSGS. Odrasle ženke SHR su podeljene u četiri grupe. Kontrolni pacovi su primili fiziološki rastvor. SHADR, SHADR+L i SHADR+L+OLE grupe su primile ADR (2 mg/kg telesne mase i.v.) dva puta u intervalu od 3 nedelje. Nakon druge injekcije, SHADR+L i SHADR+L+OLE grupe su primale gavažom L (10 mg/kg/dan), odnosno L+OLE (10+80 mg/kg/dan). Na kraju 6-nedeljnog tretmana analizirali smo albuminuriju, nivo azot monoksida (NO,), 6-nitrotriptofan (marker nitracije proteina), klotho i histopatologiju bubrega. U model grupi uočeno je mačajno povećanje albuminurije i indeksa oitećenja bubrega, ito je bilo praćeno značajno smanjenom ekspresijom klotho proteina i 6-nitortriptofana, kao i nivoa NO, u homogenatu bubrega u poredenju sa ovim vrednostima u kontrolnoj grupi. Tretman losartanom je mačajno smanjio albuminuriju, glomerulosklerozu i tubulointersticijska oštećenja u poredenju sa model grupom, ali su oštećenja bubrega i dalje bila mačajno veća nego u kontrolnoj grupi. Takode, vrednost NO, je vraćena na kontrolni nivo, a proteinska ekspresija 6-nitrotriptofana je mačajno povećana, dok je klotho-a ostala nepromenjena, u poredenju sa model grupom. Interesantno, kada je tretmanu losartanom dodat ekstrakt lista masline primećeno je mačajno i još jače smanjenje albuminurije, glomeruloskleroze, tubulointersticijske inflamacije i fibroze u poredenju sa model grupom. Takode, ovi parametri su sniženi do nivoa koji se nisu mačajno razlikovali od onih u kontroli. Pored toga, ekspresija proteina klotho-a u bubrezima je mačajno povećana u poredenju sa grupama SHADR+L i model Naši rezultati su pokazali da je ekstrakt lista masline kombinovan sa losartanom bio efikasniji od samog tretmana losartanom u usporavanju progresije FSGS kod SHR, a ovaj povoljan efekat uključivao je Klotho signalni put., Previous studies have shown the anti-inflammatory, antifibrotic and antioxidant properties of klotho, and a direct positive correlation between klotho and albuminuria in some chronic kidney disease (CKD) animal models. Focal segmented glomerulosclerosis (FSGS), a form of CKD, is distinguished by proteinuria, hypoalbuminemia, and oxidative stress. We previously showed that blockade of angiotensin Õ type 1 receptor with losartan (L) decreased proteinuria, glomerulosclerosis, tubulointerstitial inflammation, and fibrosis in animal model of FSGS induced by Adriamycin (ADR, anti-cancer agent) in spontaneously hypertensive rats (SHR), but these renal alterations were still higher than control values. Olive (Olea europaea L.) leaf extract (OLE), well-known as natural antioxidant, is rich in phenolic compounds that are thought to be responsible for its powerful antioxidant and anti-inflammatory properties. Here, we examined if losartan treatment in combination with olive leaf extract consumption could be more effective in slowing down the progression of FSGS. Adult female SHRs were divided in four groups. Control rats received vehicle. SHADR, SHADR+L and SHADR+L+OLE groups received ADR (2 mg/kg body weight i.v.) twice in 3-week-interval. After the second injection, SHADR+L, and SHADR+L+OLE groups received by gavage L (10 mg/kg/day), and L+OLE (10+80 mg/kg/day), respectively. At the end of6-week treatment, we analysed albuminuria, renal nitric oxide level (NO,), 6-nitrotryptophan (marker ofnitration of proteins), klotho, and renal histopathology In model group we found significantly increased albuininuria and renal injury score, followed by significantly decreased klotho protein expressions, NOx level, and 6- nitortryptophan in comparison to these values in control group. Losartan treatment significantly reduced albuminuria, glomerulosclerosis, and tubulointerstitial injuries compared to model group, but renal alterations were still significantly higher than in control group. Furthermore, NO, level reversed to control and 6-nitrotryptophan protein expression significantly increased, but klotho remained unchanged, compared to model group. Interestingly, when olive leaf extract was added to losartan treatment, a significant and even stronger decrease of albuminuria, glomerulosclerosis, tubulointerstitial inflammation and fibrosis were observed in comparison to model group. Also, these parameters were lowered to the levels not significantly different than in control. Furthermore, renal protein expression of klotho was significantly increased compared to SHADR+L and model groups. Our results showed that olive leaf extract in addition to losartan treatment was more effective than losartan alone in slowing down the progression of FSGS in SHR, and that beneficial effect involved the klotho signalling pathway.",
publisher = "Niš: Punta",
journal = "Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book",
title = "Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho, Ekstrakt lista masline u kombinaciji sa Losartanom ima pozitivan efekat na albuminuriju i progresivnu fokalnu segmentalnu glomerulosklerozu u hipertenzivnih miševa: uloga Klotho",
pages = "159-153",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1377"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Vajić, U. J., Grujić-Milanović, J., Životić, M., Dekanski, D.,& Jovović, Đ.. (2023). Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book
Niš: Punta., 153-159.
https://hdl.handle.net/21.15107/rcub_rimi_1377

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Vajić UJ, Grujić-Milanović J, Životić M, Dekanski D, Jovović Đ. Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book. 2023;:153-159.
https://hdl.handle.net/21.15107/rcub_rimi_1377 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Grujić-Milanović, Jelica, Životić, Maja, Dekanski, Dragana, Jovović, Đurđica, "Olive leaf extract in addition to losartan treatment improves albuminuria and progression of focal segmental glomerulosclerosis in hypertensive rats: the role of Klotho" in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia - Abstract Book (2023):153-159,
https://hdl.handle.net/21.15107/rcub_rimi_1377 .

TY  - CONF
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Vajić, Una Jovana
AU  - Miloradović, Zoran
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1433
PB  - Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije
C3  - Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
T1  - Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima
EP  - 76
IS  - 1 (supplement 1)
SP  - 75
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1433
ER  - 

@conference{
author = "Mihailović-Stanojević, Nevena and Karanović, Danijela and Grujić-Milanović, Jelica and Ivanov, Milan and Jovović, Đurđica and Vajić, Una Jovana and Miloradović, Zoran",
year = "2023",
publisher = "Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije",
journal = "Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023",
title = "Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima",
pages = "76-75",
number = "1 (supplement 1)",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1433"
}

Mihailović-Stanojević, N., Karanović, D., Grujić-Milanović, J., Ivanov, M., Jovović, Đ., Vajić, U. J.,& Miloradović, Z.. (2023). Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023
Beograd : Klinički centar Srbije - Klinika za psihijatriju i Udruženje psihijatara Srbije., 45(1 (supplement 1)), 75-76.
https://hdl.handle.net/21.15107/rcub_rimi_1433

Mihailović-Stanojević N, Karanović D, Grujić-Milanović J, Ivanov M, Jovović Đ, Vajić UJ, Miloradović Z. Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima. in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023. 2023;45(1 (supplement 1)):75-76.
https://hdl.handle.net/21.15107/rcub_rimi_1433 .

Mihailović-Stanojević, Nevena, Karanović, Danijela, Grujić-Milanović, Jelica, Ivanov, Milan, Jovović, Đurđica, Vajić, Una Jovana, Miloradović, Zoran, "Terapijski potencijal ruzmarinske kiseline u kardiovaskularnim bolestima" in Engrami - 15. Kongres farmakologa Srbije, Vrnjačka Banja, 14 - 16. septembar 2023, 45, no. 1 (supplement 1) (2023):75-76,
https://hdl.handle.net/21.15107/rcub_rimi_1433 .

TY  - CONF
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Životić, Maja
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1373
PB  - "Victor Babes" University of medicine and pharmacy from Timisoara
C3  - 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
T1  - Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?
EP  - 134
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1373
ER  - 

@conference{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Grujić-Milanović, Jelica and Životić, Maja and Dekanski, Dragana and Jovović, Đurđica",
year = "2023",
publisher = ""Victor Babes" University of medicine and pharmacy from Timisoara",
journal = "9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book",
title = "Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1373"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Vajić, U. J., Grujić-Milanović, J., Životić, M., Dekanski, D.,& Jovović, Đ.. (2023). Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
"Victor Babes" University of medicine and pharmacy from Timisoara., 134-134.
https://hdl.handle.net/21.15107/rcub_rimi_1373

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Vajić UJ, Grujić-Milanović J, Životić M, Dekanski D, Jovović Đ. Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book. 2023;:134-134.
https://hdl.handle.net/21.15107/rcub_rimi_1373 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Grujić-Milanović, Jelica, Životić, Maja, Dekanski, Dragana, Jovović, Đurđica, "Natural versus synthetic antioxidant supplementation of losartan treatment in experimental model of hypertension and chronic kidney disease: Is together better?" in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book (2023):134-134,
https://hdl.handle.net/21.15107/rcub_rimi_1373 .

TY  - JOUR
AU  - Kurćubić, Ivana
AU  - Đuriš, Jelena
AU  - Cvijić, Sandra
AU  - Crevar, Milkica
AU  - Ibrić, Svetlana
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Vajić, Una Jovana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1316
AB  - In order to exploit the advantage of drug delivery through the buccal mucosa, mucoadhesive buccal films with propranolol hydrochloride based on polyethylene oxide, hydroxypropyl methylcellulose, and polyvinyl alcohol have been developed. The aim of this study was the development, pharmaceutical-technological (uniformity of mass and drug content, film thickness, potential interactions between polymers and propranolol hydrochloride, in vitro drug permeation and drug release, mechanical and mucoadhesive properties), and biopharmaceutical characterization of prepared mucoadhesive buccal films through conducting in vivo study in spontaneously hypertensive rats and in silico modeling of intraoral and gastrointestinal drug absorption. For in vivo study formulation F2 (hydroxypropylmethyl cellulose 0.5%, polyethylene oxide 3.5%, polyvinyl alcohol 1.5%, propylene glycol 3%, and propranolol hydrochloride 2%) was selected, which showed the highest values of tensile strength and percentage of elongation, as well as the highest value of the force of adhesion. Results of pharmacokinetic in rats and in silico modeling confirmed the superiority of the mucoadhesive buccal films over immediate-release tablets (in rats: AUC0→24h 66.13 ± 18.03 vs. 24.61 ± 5.52 μg⋅h/mL, AUC0→∞ 111.82 ± 39.04 vs. 47.85 ± 11.67 μg⋅h/mL; in silico: AUC0→24h 200.17 vs.74.58 ng⋅h/mL, AUC0→∞ 204.04 vs. 75.64 ng⋅h/mL). Hemodynamic measurements have shown that mucoadhesive buccal films, compared to immediate-release tablets, provide a more pronounced decrease primarily in heart rate (28-51%), but also in diastolic pressure (up to 33%), as well as a longer heart rate reduction that was maintained for up to 12th h. Therefore, mucoadhesive buccal films increased the degree of absorbed drug and thus contributed to better therapeutic outcomes compared to immediate-release tablets for peroral administration.
PB  - Elsevier
T2  - Journal of Drug Delivery Science and Technology
T1  - Integrated in vitro – in vivo – in silico studies in the pharmaceutical development of propranolol hydrochloride mucoadhesive buccal films
SP  - 104715
VL  - 86
DO  - 10.1016/j.jddst.2023.104715
ER  - 

@article{
author = "Kurćubić, Ivana and Đuriš, Jelena and Cvijić, Sandra and Crevar, Milkica and Ibrić, Svetlana and Miloradović, Zoran and Mihailović-Stanojević, Nevena and Karanović, Danijela and Ivanov, Milan and Jovović, Đurđica and Vajić, Una Jovana",
year = "2023",
abstract = "In order to exploit the advantage of drug delivery through the buccal mucosa, mucoadhesive buccal films with propranolol hydrochloride based on polyethylene oxide, hydroxypropyl methylcellulose, and polyvinyl alcohol have been developed. The aim of this study was the development, pharmaceutical-technological (uniformity of mass and drug content, film thickness, potential interactions between polymers and propranolol hydrochloride, in vitro drug permeation and drug release, mechanical and mucoadhesive properties), and biopharmaceutical characterization of prepared mucoadhesive buccal films through conducting in vivo study in spontaneously hypertensive rats and in silico modeling of intraoral and gastrointestinal drug absorption. For in vivo study formulation F2 (hydroxypropylmethyl cellulose 0.5%, polyethylene oxide 3.5%, polyvinyl alcohol 1.5%, propylene glycol 3%, and propranolol hydrochloride 2%) was selected, which showed the highest values of tensile strength and percentage of elongation, as well as the highest value of the force of adhesion. Results of pharmacokinetic in rats and in silico modeling confirmed the superiority of the mucoadhesive buccal films over immediate-release tablets (in rats: AUC0→24h 66.13 ± 18.03 vs. 24.61 ± 5.52 μg⋅h/mL, AUC0→∞ 111.82 ± 39.04 vs. 47.85 ± 11.67 μg⋅h/mL; in silico: AUC0→24h 200.17 vs.74.58 ng⋅h/mL, AUC0→∞ 204.04 vs. 75.64 ng⋅h/mL). Hemodynamic measurements have shown that mucoadhesive buccal films, compared to immediate-release tablets, provide a more pronounced decrease primarily in heart rate (28-51%), but also in diastolic pressure (up to 33%), as well as a longer heart rate reduction that was maintained for up to 12th h. Therefore, mucoadhesive buccal films increased the degree of absorbed drug and thus contributed to better therapeutic outcomes compared to immediate-release tablets for peroral administration.",
publisher = "Elsevier",
journal = "Journal of Drug Delivery Science and Technology",
title = "Integrated in vitro – in vivo – in silico studies in the pharmaceutical development of propranolol hydrochloride mucoadhesive buccal films",
pages = "104715",
volume = "86",
doi = "10.1016/j.jddst.2023.104715"
}

Kurćubić, I., Đuriš, J., Cvijić, S., Crevar, M., Ibrić, S., Miloradović, Z., Mihailović-Stanojević, N., Karanović, D., Ivanov, M., Jovović, Đ.,& Vajić, U. J.. (2023). Integrated in vitro – in vivo – in silico studies in the pharmaceutical development of propranolol hydrochloride mucoadhesive buccal films. in Journal of Drug Delivery Science and Technology
Elsevier., 86, 104715.
https://doi.org/10.1016/j.jddst.2023.104715

Kurćubić I, Đuriš J, Cvijić S, Crevar M, Ibrić S, Miloradović Z, Mihailović-Stanojević N, Karanović D, Ivanov M, Jovović Đ, Vajić UJ. Integrated in vitro – in vivo – in silico studies in the pharmaceutical development of propranolol hydrochloride mucoadhesive buccal films. in Journal of Drug Delivery Science and Technology. 2023;86:104715.
doi:10.1016/j.jddst.2023.104715 .

Kurćubić, Ivana, Đuriš, Jelena, Cvijić, Sandra, Crevar, Milkica, Ibrić, Svetlana, Miloradović, Zoran, Mihailović-Stanojević, Nevena, Karanović, Danijela, Ivanov, Milan, Jovović, Đurđica, Vajić, Una Jovana, "Integrated in vitro – in vivo – in silico studies in the pharmaceutical development of propranolol hydrochloride mucoadhesive buccal films" in Journal of Drug Delivery Science and Technology, 86 (2023):104715,
https://doi.org/10.1016/j.jddst.2023.104715 . .

TY  - CONF
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Vajić, Una Jovana
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Miloradović, Zoran
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1371
PB  - "Victor Babes" University of medicine and pharmacy from Timisoara
C3  - 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
T1  - Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1371
ER  - 

@conference{
author = "Mihailović-Stanojević, Nevena and Karanović, Danijela and Grujić-Milanović, Jelica and Vajić, Una Jovana and Ivanov, Milan and Jovović, Đurđica and Miloradović, Zoran",
year = "2023",
publisher = ""Victor Babes" University of medicine and pharmacy from Timisoara",
journal = "9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book",
title = "Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1371"
}

Mihailović-Stanojević, N., Karanović, D., Grujić-Milanović, J., Vajić, U. J., Ivanov, M., Jovović, Đ.,& Miloradović, Z.. (2023). Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
"Victor Babes" University of medicine and pharmacy from Timisoara., 82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1371

Mihailović-Stanojević N, Karanović D, Grujić-Milanović J, Vajić UJ, Ivanov M, Jovović Đ, Miloradović Z. Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book. 2023;:82-82.
https://hdl.handle.net/21.15107/rcub_rimi_1371 .

Mihailović-Stanojević, Nevena, Karanović, Danijela, Grujić-Milanović, Jelica, Vajić, Una Jovana, Ivanov, Milan, Jovović, Đurđica, Miloradović, Zoran, "Caffeic acid improves antioxidant defense and attenuates oxidative stress in heart of spontaneously hypersensitive rat" in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book (2023):82-82,
https://hdl.handle.net/21.15107/rcub_rimi_1371 .

TY  - CONF
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1372
PB  - "Victor Babes" University of medicine and pharmacy from Timisoara
C3  - 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
T1  - Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats
EP  - 131
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1372
ER  - 

@conference{
author = "Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Ivanov, Milan and Karanović, Danijela and Jovović, Đurđica and Grujić-Milanović, Jelica and Miloradović, Zoran",
year = "2023",
publisher = ""Victor Babes" University of medicine and pharmacy from Timisoara",
journal = "9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book",
title = "Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats",
pages = "131-131",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1372"
}

Vajić, U. J., Mihailović-Stanojević, N., Ivanov, M., Karanović, D., Jovović, Đ., Grujić-Milanović, J.,& Miloradović, Z.. (2023). Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book
"Victor Babes" University of medicine and pharmacy from Timisoara., 131-131.
https://hdl.handle.net/21.15107/rcub_rimi_1372

Vajić UJ, Mihailović-Stanojević N, Ivanov M, Karanović D, Jovović Đ, Grujić-Milanović J, Miloradović Z. Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats. in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book. 2023;:131-131.
https://hdl.handle.net/21.15107/rcub_rimi_1372 .

Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Ivanov, Milan, Karanović, Danijela, Jovović, Đurđica, Grujić-Milanović, Jelica, Miloradović, Zoran, "Effects of chronic supplementation with Urtica dioica L. leaf extract and chlorogenic acid on hemodynamic and biochemical parameters in spontaneously hypertensive rats" in 9th European Section Meeting of the International Academy of Cardiovascular Sciences, October 4th – 7th 2023, Timișoara, Romania - Programme & Abstract Book (2023):131-131,
https://hdl.handle.net/21.15107/rcub_rimi_1372 .

TY  - CONF
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Životić, Maja
AU  - Ivanov, Milan
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1378
PB  - Niš: Punta
C3  - Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
T1  - The role of NADPH oxidase and angiotensin II receptor type 2 in developing acute kidney injury in experimental hypertension
T1  - Uloga NADPH oksidaze i receptora za angiotenzin H tip 2 u razvoju akutne bubrežne slabosti u eksperimentalnoj hipertenziji
EP  - 150
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1378
ER  - 

@conference{
author = "Miloradović, Zoran and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Životić, Maja and Ivanov, Milan",
year = "2023",
publisher = "Niš: Punta",
journal = "Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia",
title = "The role of NADPH oxidase and angiotensin II receptor type 2 in developing acute kidney injury in experimental hypertension, Uloga NADPH oksidaze i receptora za angiotenzin H tip 2 u razvoju akutne bubrežne slabosti u eksperimentalnoj hipertenziji",
pages = "150-149",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1378"
}

Miloradović, Z., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ., Životić, M.,& Ivanov, M.. (2023). The role of NADPH oxidase and angiotensin II receptor type 2 in developing acute kidney injury in experimental hypertension. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
Niš: Punta., 149-150.
https://hdl.handle.net/21.15107/rcub_rimi_1378

Miloradović Z, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Životić M, Ivanov M. The role of NADPH oxidase and angiotensin II receptor type 2 in developing acute kidney injury in experimental hypertension. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia. 2023;:149-150.
https://hdl.handle.net/21.15107/rcub_rimi_1378 .

Miloradović, Zoran, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Životić, Maja, Ivanov, Milan, "The role of NADPH oxidase and angiotensin II receptor type 2 in developing acute kidney injury in experimental hypertension" in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia (2023):149-150,
https://hdl.handle.net/21.15107/rcub_rimi_1378 .

TY  - CONF
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Mihailović-Stanojević, Nevena
AU  - Grujić-Milanović, Jelica
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Marković-Lipkovski, Jasmina
AU  - Jovović, Đurđica
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1376
AB  - Hipertenzija, koja se izmedu ostalog karakteriše i deficitom vasodilatatornog azotnog monoksida
(AM), mačajno pogoršava kliničku sliku postishernične akutne bubrežne slabosti (ABS) kod ljudi. Ponovno vraćanje krvi u bubrežno tkivo, samo po sebi dovodi do masovne produkcije slobodnih vrsta kiseonika (SVK) i pogoršanja ishemijske povrede. Ispitivali smo potencijalni zaštitni efekat primene donora AM-a, kao i uklanjanja SVK na sistemske i bubrežne hemodinamske parametre, kao i na strukturu i funkciju bubrega pacova sa urodenom hipertenzijom (PUH), kojima je izazivana postishemična ABS. Za navedene potrebe, PUH su dobijali donor AM, L-arginin, prirodni sakupljač SRK vitamin C (Vit C) ili sintetski mimetik enzima superoksid dismutase TEMPOL, tokom ishemije-reperfuzije bubrega u trajanju od 40 minuta. Tretman L-argininom je poboljšao sistemsku i bubreżnu hemodinarniku, ali nije uspeo da smanji ośtećenje bubrega. Nasuprot tome, intenzitet nekroze i dilatacije tubula su bili mačajno smanjeni kod pacova u ABS+Vit C grupi u poredenju sa kontrolnom ABS grupom. Uprkos smanjenju lipidne peroksidacije u plazmi, kao i renalnog vaskularnog otpora, tretman TEMPOL-om nije uspeo da ublaži reperfuzijom nastala oštećenja bubreżnog tkiva kod PUH. Naši rezultati ukazuju na to da SVK učestvuju u reperfuzionim oštećenjima bubrega, a da su sintetski mimetik superoksid dismutaze i donor AM neefikasni u zaštiti glomerula i tubula nakon nastanka ABS kod PUH. Sa druge strane, naši rezultati sugerišu daje Vit C, prirodni antioksidant, hemoprotektant umerenog zaśtitnog dejstva protiv ishemijsko-reperfuzionih povreda bubrega u hipertenzivnim okolnostima. Ova zaštita je većinski povezana sa direktnim uklanjanjem SVK.
AB  - Hypertension, which is among others characterized by reducing of nitric oxide (NO) mediated vasodilatation, strongly aggravates postischemic acute kidney injury (AKI) in humans. Kidney reperfusion itself increases ischemic injury through the massive production of oxygen-free radicals. We investigated protective potential of NO supplementation and superoxide radical scavenging on systemic and renal haemodynamic parameters as well as kidney function and structure in spontaneously hypertensive rats (SHR) with induced postischemic AKI. For these purposes, SHR were subjected to NO donor L-arginine, natural oxidant scavenger vitamin C (Vit C) or synthetic superoxide dismutase mimetic TEMPOL during 40 minutes kidney ischaemia-reperfusion period. L-arginine supplementation improves systemic and renal hemodynamic but failed to reduce kidney tubular injuries. In opposite, intensity of tubular necrosis and dilatation was markedly reduced in AKI+Vit C group in comparison to AKI control. Despite blunting of lipid peroxidation in plasma and diminishing of renal vascular resistance, TEMPOL treatment also failed to reduce kidney tissue injuries induced by reperfusion in SHR. Our results suggest that superoxide radicals participate in kidney reperfusion injuries while synthetic superoxide dismutase and NO supplementation are ineffective in glomerular and tubular protection after AKI episode in hypertensive rats. On the other hand, our results promote natural antioxidant, vitamin C, as a moderately effective chemoprotectant against kidney ischemia-reperfusion injuries in hypertensive surrounding. Protection is mainly related to the direct superoxide radical scavenging.
PB  - Niš : Punta
C3  - Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
T1  - Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension
T1  - Bioraspoloživost azotnog monoksida i antioksidativna odbrana kao ciljevi terapije u udruženom modelu akutne bubrežne slabosti i hipertenzije
EP  - 22
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1376
ER  - 

@conference{
author = "Miloradović, Zoran and Ivanov, Milan and Mihailović-Stanojević, Nevena and Grujić-Milanović, Jelica and Karanović, Danijela and Vajić, Una Jovana and Marković-Lipkovski, Jasmina and Jovović, Đurđica",
year = "2023",
abstract = "Hipertenzija, koja se izmedu ostalog karakteriše i deficitom vasodilatatornog azotnog monoksida
(AM), mačajno pogoršava kliničku sliku postishernične akutne bubrežne slabosti (ABS) kod ljudi. Ponovno vraćanje krvi u bubrežno tkivo, samo po sebi dovodi do masovne produkcije slobodnih vrsta kiseonika (SVK) i pogoršanja ishemijske povrede. Ispitivali smo potencijalni zaštitni efekat primene donora AM-a, kao i uklanjanja SVK na sistemske i bubrežne hemodinamske parametre, kao i na strukturu i funkciju bubrega pacova sa urodenom hipertenzijom (PUH), kojima je izazivana postishemična ABS. Za navedene potrebe, PUH su dobijali donor AM, L-arginin, prirodni sakupljač SRK vitamin C (Vit C) ili sintetski mimetik enzima superoksid dismutase TEMPOL, tokom ishemije-reperfuzije bubrega u trajanju od 40 minuta. Tretman L-argininom je poboljšao sistemsku i bubreżnu hemodinarniku, ali nije uspeo da smanji ośtećenje bubrega. Nasuprot tome, intenzitet nekroze i dilatacije tubula su bili mačajno smanjeni kod pacova u ABS+Vit C grupi u poredenju sa kontrolnom ABS grupom. Uprkos smanjenju lipidne peroksidacije u plazmi, kao i renalnog vaskularnog otpora, tretman TEMPOL-om nije uspeo da ublaži reperfuzijom nastala oštećenja bubreżnog tkiva kod PUH. Naši rezultati ukazuju na to da SVK učestvuju u reperfuzionim oštećenjima bubrega, a da su sintetski mimetik superoksid dismutaze i donor AM neefikasni u zaštiti glomerula i tubula nakon nastanka ABS kod PUH. Sa druge strane, naši rezultati sugerišu daje Vit C, prirodni antioksidant, hemoprotektant umerenog zaśtitnog dejstva protiv ishemijsko-reperfuzionih povreda bubrega u hipertenzivnim okolnostima. Ova zaštita je većinski povezana sa direktnim uklanjanjem SVK., Hypertension, which is among others characterized by reducing of nitric oxide (NO) mediated vasodilatation, strongly aggravates postischemic acute kidney injury (AKI) in humans. Kidney reperfusion itself increases ischemic injury through the massive production of oxygen-free radicals. We investigated protective potential of NO supplementation and superoxide radical scavenging on systemic and renal haemodynamic parameters as well as kidney function and structure in spontaneously hypertensive rats (SHR) with induced postischemic AKI. For these purposes, SHR were subjected to NO donor L-arginine, natural oxidant scavenger vitamin C (Vit C) or synthetic superoxide dismutase mimetic TEMPOL during 40 minutes kidney ischaemia-reperfusion period. L-arginine supplementation improves systemic and renal hemodynamic but failed to reduce kidney tubular injuries. In opposite, intensity of tubular necrosis and dilatation was markedly reduced in AKI+Vit C group in comparison to AKI control. Despite blunting of lipid peroxidation in plasma and diminishing of renal vascular resistance, TEMPOL treatment also failed to reduce kidney tissue injuries induced by reperfusion in SHR. Our results suggest that superoxide radicals participate in kidney reperfusion injuries while synthetic superoxide dismutase and NO supplementation are ineffective in glomerular and tubular protection after AKI episode in hypertensive rats. On the other hand, our results promote natural antioxidant, vitamin C, as a moderately effective chemoprotectant against kidney ischemia-reperfusion injuries in hypertensive surrounding. Protection is mainly related to the direct superoxide radical scavenging.",
publisher = "Niš : Punta",
journal = "Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia",
title = "Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension, Bioraspoloživost azotnog monoksida i antioksidativna odbrana kao ciljevi terapije u udruženom modelu akutne bubrežne slabosti i hipertenzije",
pages = "22-16",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1376"
}

Miloradović, Z., Ivanov, M., Mihailović-Stanojević, N., Grujić-Milanović, J., Karanović, D., Vajić, U. J., Marković-Lipkovski, J.,& Jovović, Đ.. (2023). Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
Niš : Punta., 16-22.
https://hdl.handle.net/21.15107/rcub_rimi_1376

Miloradović Z, Ivanov M, Mihailović-Stanojević N, Grujić-Milanović J, Karanović D, Vajić UJ, Marković-Lipkovski J, Jovović Đ. Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia. 2023;:16-22.
https://hdl.handle.net/21.15107/rcub_rimi_1376 .

Miloradović, Zoran, Ivanov, Milan, Mihailović-Stanojević, Nevena, Grujić-Milanović, Jelica, Karanović, Danijela, Vajić, Una Jovana, Marković-Lipkovski, Jasmina, Jovović, Đurđica, "Nitric oxide bioavailability and antioxidant defence as therapeutic targets in synergistic model of acute kidney injury and hypertension" in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia (2023):16-22,
https://hdl.handle.net/21.15107/rcub_rimi_1376 .

TY  - JOUR
AU  - Nešović-Ostojić, Jelena
AU  - Životić, Maja
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Brkić, Predrag
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Radojević-Škodrić, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1295
AB  - Ischemia–reperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variants’ (H2AX, γH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemia–reperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of γH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of γH2AX. In addition, we observed that different structures dominated when γH2AX and H2AXY142ph expression levels were compared. γH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acid–Schiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition.
T2  - International Journal of Molecular Sciences
T1  - Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury
IS  - 9
SP  - 8085
VL  - 24
DO  - 10.3390/ijms24098085
ER  - 

@article{
author = "Nešović-Ostojić, Jelena and Životić, Maja and Kovačević, Sanjin and Ivanov, Milan and Brkić, Predrag and Mihailović-Stanojević, Nevena and Karanović, Danijela and Vajić, Una Jovana and Miloradović, Zoran and Jovović, Đurđica and Radojević-Škodrić, Sanja",
year = "2023",
abstract = "Ischemia–reperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variants’ (H2AX, γH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemia–reperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of γH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of γH2AX. In addition, we observed that different structures dominated when γH2AX and H2AXY142ph expression levels were compared. γH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acid–Schiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition.",
journal = "International Journal of Molecular Sciences",
title = "Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury",
number = "9",
pages = "8085",
volume = "24",
doi = "10.3390/ijms24098085"
}

Nešović-Ostojić, J., Životić, M., Kovačević, S., Ivanov, M., Brkić, P., Mihailović-Stanojević, N., Karanović, D., Vajić, U. J., Miloradović, Z., Jovović, Đ.,& Radojević-Škodrić, S.. (2023). Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury. in International Journal of Molecular Sciences, 24(9), 8085.
https://doi.org/10.3390/ijms24098085

Nešović-Ostojić J, Životić M, Kovačević S, Ivanov M, Brkić P, Mihailović-Stanojević N, Karanović D, Vajić UJ, Miloradović Z, Jovović Đ, Radojević-Škodrić S. Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury. in International Journal of Molecular Sciences. 2023;24(9):8085.
doi:10.3390/ijms24098085 .

Nešović-Ostojić, Jelena, Životić, Maja, Kovačević, Sanjin, Ivanov, Milan, Brkić, Predrag, Mihailović-Stanojević, Nevena, Karanović, Danijela, Vajić, Una Jovana, Miloradović, Zoran, Jovović, Đurđica, Radojević-Škodrić, Sanja, "Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury" in International Journal of Molecular Sciences, 24, no. 9 (2023):8085,
https://doi.org/10.3390/ijms24098085 . .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Jaćević, Vesna
AU  - Miloradović, Zoran
AU  - Milanović, Slađan
AU  - Jovović, Đurđica
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1263
AB  - Objective Background: The main cause of death among patients with malignant hypertension is a kidney failure. The promising field in essential and malignant hypertension therapy could be centered on the amelioration of oxidative stress using antioxidant molecules like resveratrol. Resveratrol is a potent antioxidative agent naturally occurred in many plants that possess health-promoting properties. Methods: In the present study, we investigated the therapeutic potential of resveratrol, a polyphenol with anti-oxidative activity, in NG-L-Arginine Methyl Ester (L-NAME) treated spontaneously hypertensive rats (SHR) - malignantly hypertensive rats (MHR). Results: Resveratrol significantly improves oxidative damages by modulation of antioxidant enzymes and suppression of prooxidant factors in the kidney tissue of MHR. Enhanced antioxidant defense in the kidney improves renal function and ameliorates the morphological changes in this target organ. Besides, protective properties of resveratrol are followed by the restoration of the nitrogen oxide (NO) pathway. 4) Conclusion: Antioxidant therapy with resveratrol could represent promising therapeutical approach in hypertension, especially malignant, against kidney damage.
T2  - Biomedicine & Pharmacotherapy
T1  - Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability
SP  - 113642
VL  - 154
DO  - 10.1016/j.biopha.2022.113642
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Jaćević, Vesna and Miloradović, Zoran and Milanović, Slađan and Jovović, Đurđica and Ivanov, Milan and Karanović, Danijela and Vajić, Una Jovana and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "Objective Background: The main cause of death among patients with malignant hypertension is a kidney failure. The promising field in essential and malignant hypertension therapy could be centered on the amelioration of oxidative stress using antioxidant molecules like resveratrol. Resveratrol is a potent antioxidative agent naturally occurred in many plants that possess health-promoting properties. Methods: In the present study, we investigated the therapeutic potential of resveratrol, a polyphenol with anti-oxidative activity, in NG-L-Arginine Methyl Ester (L-NAME) treated spontaneously hypertensive rats (SHR) - malignantly hypertensive rats (MHR). Results: Resveratrol significantly improves oxidative damages by modulation of antioxidant enzymes and suppression of prooxidant factors in the kidney tissue of MHR. Enhanced antioxidant defense in the kidney improves renal function and ameliorates the morphological changes in this target organ. Besides, protective properties of resveratrol are followed by the restoration of the nitrogen oxide (NO) pathway. 4) Conclusion: Antioxidant therapy with resveratrol could represent promising therapeutical approach in hypertension, especially malignant, against kidney damage.",
journal = "Biomedicine & Pharmacotherapy",
title = "Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability",
pages = "113642",
volume = "154",
doi = "10.1016/j.biopha.2022.113642"
}

Grujić-Milanović, J., Jaćević, V., Miloradović, Z., Milanović, S., Jovović, Đ., Ivanov, M., Karanović, D., Vajić, U. J.,& Mihailović-Stanojević, N.. (2022). Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability. in Biomedicine & Pharmacotherapy, 154, 113642.
https://doi.org/10.1016/j.biopha.2022.113642

Grujić-Milanović J, Jaćević V, Miloradović Z, Milanović S, Jovović Đ, Ivanov M, Karanović D, Vajić UJ, Mihailović-Stanojević N. Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability. in Biomedicine & Pharmacotherapy. 2022;154:113642.
doi:10.1016/j.biopha.2022.113642 .

Grujić-Milanović, Jelica, Jaćević, Vesna, Miloradović, Zoran, Milanović, Slađan, Jovović, Đurđica, Ivanov, Milan, Karanović, Danijela, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, "Resveratrol improved kidney function and structure in malignantly hypertensive rats by restoration of antioxidant capacity and nitric oxide bioavailability" in Biomedicine & Pharmacotherapy, 154 (2022):113642,
https://doi.org/10.1016/j.biopha.2022.113642 . .

TY  - CONF
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1440
AB  - Objective: 
Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). 4-hydroxynonenal (4-HNE) is a product of lipid peroxidation and may serve as non-invasive biomarker of oxidative stress while neutrophil gelatinase-associated lipocalin (NGAL) is a biomarker of AKI. Hypoxia-inducible factors (HIFs) are the “master’’ transcription factors responsible for gene expression in hypoxia, which play an important role in kidney injury and repair by regulating HIF target genes, including microRNAs.
Design and method: 
The study was conducted on male SHR rats, randomly divided into following groups: sham-operated group (SHAM) AKI control group, AKI group with apocynin treatment (AKI+APO), group with HBO preconditioning before AKI induction (AKI+HBO) and group with HBO preconditioning before and apocynin treatment after AKI induction (AKI+APO+HBO). HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24 h before AKI induction. Apocynin, NADPH oxidase inhibitor was injected 5 minutes before reperfusion.
Results: 
AKI induction significantly decreased creatinine (CCr), urea (CU) and phosphate (CPhos) clearances when compared to SHAM group. Remarkable increase in CCr, CU, CPhos levels were observed in all treated groups (AKI+APO, AKI+HBO, AKI+APO+HBO) in comparison to AKI group. Sham – operated rats did not express 4-HNE and NGAL in any parenchymal structure. AKI induced abundant and strong glomerular expression of 4-HNE along with expression in interstitial compartment. All treatments (AKI+APO, AKI+HBO, AKI+APO+HBO) significantly decreased 4-HNE expression both in glomeruli and interstitium. Also, AKI stimulated widespread NGAL expression in renal epithelial tubular cells with fine granular appearance on the apical surface of the cells, affecting entire circumference of tubular cross-sectioning, which was decreased in all treated groups. In SHAM group, the expression of HIF1 alpha and HIF2 alpha was focal with weak intensity in glomeruli and interstitium. In AKI group, the expression was diffuse and strong, with remarkable improvement in all treated groups.
Conclusions: 
Considering our results, we can conclude that apocynin treatment, as well as HBO preconditioning may have protective effects in postischemic acute kidney injury induced in spontaneously hypertensive rats.
PB  - Wolters Kluwer Health, Inc.
C3  - Journal of Hypertension
T1  - Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats
IS  - Suppl 1
SP  - e251
VL  - 40
DO  - 10.1097/01.hjh.0000838064.87501.71
ER  - 

@conference{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2022",
abstract = "Objective: 
Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). 4-hydroxynonenal (4-HNE) is a product of lipid peroxidation and may serve as non-invasive biomarker of oxidative stress while neutrophil gelatinase-associated lipocalin (NGAL) is a biomarker of AKI. Hypoxia-inducible factors (HIFs) are the “master’’ transcription factors responsible for gene expression in hypoxia, which play an important role in kidney injury and repair by regulating HIF target genes, including microRNAs.
Design and method: 
The study was conducted on male SHR rats, randomly divided into following groups: sham-operated group (SHAM) AKI control group, AKI group with apocynin treatment (AKI+APO), group with HBO preconditioning before AKI induction (AKI+HBO) and group with HBO preconditioning before and apocynin treatment after AKI induction (AKI+APO+HBO). HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24 h before AKI induction. Apocynin, NADPH oxidase inhibitor was injected 5 minutes before reperfusion.
Results: 
AKI induction significantly decreased creatinine (CCr), urea (CU) and phosphate (CPhos) clearances when compared to SHAM group. Remarkable increase in CCr, CU, CPhos levels were observed in all treated groups (AKI+APO, AKI+HBO, AKI+APO+HBO) in comparison to AKI group. Sham – operated rats did not express 4-HNE and NGAL in any parenchymal structure. AKI induced abundant and strong glomerular expression of 4-HNE along with expression in interstitial compartment. All treatments (AKI+APO, AKI+HBO, AKI+APO+HBO) significantly decreased 4-HNE expression both in glomeruli and interstitium. Also, AKI stimulated widespread NGAL expression in renal epithelial tubular cells with fine granular appearance on the apical surface of the cells, affecting entire circumference of tubular cross-sectioning, which was decreased in all treated groups. In SHAM group, the expression of HIF1 alpha and HIF2 alpha was focal with weak intensity in glomeruli and interstitium. In AKI group, the expression was diffuse and strong, with remarkable improvement in all treated groups.
Conclusions: 
Considering our results, we can conclude that apocynin treatment, as well as HBO preconditioning may have protective effects in postischemic acute kidney injury induced in spontaneously hypertensive rats.",
publisher = "Wolters Kluwer Health, Inc.",
journal = "Journal of Hypertension",
title = "Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats",
number = "Suppl 1",
pages = "e251",
volume = "40",
doi = "10.1097/01.hjh.0000838064.87501.71"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović Ostojić, J.. (2022). Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats. in Journal of Hypertension
Wolters Kluwer Health, Inc.., 40(Suppl 1), e251.
https://doi.org/10.1097/01.hjh.0000838064.87501.71

Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Nešović Ostojić J. Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats. in Journal of Hypertension. 2022;40(Suppl 1):e251.
doi:10.1097/01.hjh.0000838064.87501.71 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović Ostojić, Jelena, "Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats" in Journal of Hypertension, 40, no. Suppl 1 (2022):e251,
https://doi.org/10.1097/01.hjh.0000838064.87501.71 . .

TY  - CONF
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Grujić-Milanović, Jelica
AU  - Vajić, Una Jovana
AU  - Jovović, Đurđica
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1438
AB  - Uvod. Reaktivne vrste kiseonika (RVK), važni signalni molekuli, u uslovima prekomerne produkcije dovode do poremećaja funkcije i strukture organa i tkiva, uključujući bubreg. RVK doprinose dijabetičnim nefropatijama, hipertenzijom i ishemijom izazvanim bolestima bubrega, kao i toksičnim nefropatijama. Cilj i metode. Proučavali smo, na eksperimentalnim modelima, uticaj RVK na nastanak i razvoj fokalno-segmentne glomerulo-skleroze i postishemične akutne bubrežne slabosti. Rezultati. Naši rezultati su pokazali štetno delovanje reperfuzijom indukovane produkcije RVK na protok krvi kroz bubrežnu arteriju, glomerusku i tubulsku funkciju bubrega, kao i strukturu bubrežnog tkiva. Sa druge strane, uočili smo brojne interakcije ovih molekula sa azotnim monoksidom, sa kojim formiraju visoko reaktivne peroksinitrite i oštećuju tkivo bubrega. Evidentna je i povezanost RVK sa renin angiotenzin sistemom, jer je blokada receptora za angiotenzin 2 dovela do poboljšanja strukture i funcije bubrega u oba modela nefropatija. Akutno uklanjanje RVK mimeticima superoksid dismutaze, kao i pretretman hiperbaričnim kiseonikom, su takođe dovodili do usporavanja toka i razvoja nefropatija, sa određenim, neočekivanim, odstupanjima u ishodima kombinovane terapije. Zaključak. RVK igraju značajnu ulogu u patofiziološkim mehanizmima nefropatija, a strategije njihovog neutralisanja predstavljaju obećavajući pristup terapiji ovih poremećaja.
PB  - Beograd: Srpsko biološko društvo
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.
T1  - Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1438
ER  - 

@conference{
author = "Miloradović, Zoran and Mihailović-Stanojević, Nevena and Ivanov, Milan and Karanović, Danijela and Grujić-Milanović, Jelica and Vajić, Una Jovana and Jovović, Đurđica",
year = "2022",
abstract = "Uvod. Reaktivne vrste kiseonika (RVK), važni signalni molekuli, u uslovima prekomerne produkcije dovode do poremećaja funkcije i strukture organa i tkiva, uključujući bubreg. RVK doprinose dijabetičnim nefropatijama, hipertenzijom i ishemijom izazvanim bolestima bubrega, kao i toksičnim nefropatijama. Cilj i metode. Proučavali smo, na eksperimentalnim modelima, uticaj RVK na nastanak i razvoj fokalno-segmentne glomerulo-skleroze i postishemične akutne bubrežne slabosti. Rezultati. Naši rezultati su pokazali štetno delovanje reperfuzijom indukovane produkcije RVK na protok krvi kroz bubrežnu arteriju, glomerusku i tubulsku funkciju bubrega, kao i strukturu bubrežnog tkiva. Sa druge strane, uočili smo brojne interakcije ovih molekula sa azotnim monoksidom, sa kojim formiraju visoko reaktivne peroksinitrite i oštećuju tkivo bubrega. Evidentna je i povezanost RVK sa renin angiotenzin sistemom, jer je blokada receptora za angiotenzin 2 dovela do poboljšanja strukture i funcije bubrega u oba modela nefropatija. Akutno uklanjanje RVK mimeticima superoksid dismutaze, kao i pretretman hiperbaričnim kiseonikom, su takođe dovodili do usporavanja toka i razvoja nefropatija, sa određenim, neočekivanim, odstupanjima u ishodima kombinovane terapije. Zaključak. RVK igraju značajnu ulogu u patofiziološkim mehanizmima nefropatija, a strategije njihovog neutralisanja predstavljaju obećavajući pristup terapiji ovih poremećaja.",
publisher = "Beograd: Srpsko biološko društvo",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.",
title = "Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja",
pages = "6",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1438"
}

Miloradović, Z., Mihailović-Stanojević, N., Ivanov, M., Karanović, D., Grujić-Milanović, J., Vajić, U. J.,& Jovović, Đ.. (2022). Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.
Beograd: Srpsko biološko društvo., 6.
https://hdl.handle.net/21.15107/rcub_rimi_1438

Miloradović Z, Mihailović-Stanojević N, Ivanov M, Karanović D, Grujić-Milanović J, Vajić UJ, Jovović Đ. Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija.. 2022;:6.
https://hdl.handle.net/21.15107/rcub_rimi_1438 .

Miloradović, Zoran, Mihailović-Stanojević, Nevena, Ivanov, Milan, Karanović, Danijela, Grujić-Milanović, Jelica, Vajić, Una Jovana, Jovović, Đurđica, "Reaktivne vrste kiseonika u patofiziologiji bubrežnih oboljenja" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Srbija. (2022):6,
https://hdl.handle.net/21.15107/rcub_rimi_1438 .

TY  - CONF
AU  - Ivanov, Milan
AU  - Kovačević, Sanjin
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1437
AB  - Kidney disease represents a serious global health problem. Free radicals and prooxidants produced during ischemic acute kidney injury (AKI) may further aggravate the course of the disease and play a role in the pathogenesis of subsequent complications. The aims of our study were to examine the importance of NADPH oxidase blockade and to determine the effect of hyperbaric oxygen preconditioning on the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. NADPH oxidase blockade was performed by Apocynin (40 mg/kg body weigh)t, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning,with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, NADPH oxidase blockade and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
PB  - European Section of the International Academy of Cardiovascular Sciences
C3  - 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book
T1  - Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat
EP  - 55
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1437
ER  - 

@conference{
author = "Ivanov, Milan and Kovačević, Sanjin and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2022",
abstract = "Kidney disease represents a serious global health problem. Free radicals and prooxidants produced during ischemic acute kidney injury (AKI) may further aggravate the course of the disease and play a role in the pathogenesis of subsequent complications. The aims of our study were to examine the importance of NADPH oxidase blockade and to determine the effect of hyperbaric oxygen preconditioning on the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. NADPH oxidase blockade was performed by Apocynin (40 mg/kg body weigh)t, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning,with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, NADPH oxidase blockade and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.",
publisher = "European Section of the International Academy of Cardiovascular Sciences",
journal = "8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book",
title = "Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat",
pages = "55-55",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1437"
}

Ivanov, M., Kovačević, S., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović Ostojić, J.. (2022). Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat. in 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book
European Section of the International Academy of Cardiovascular Sciences., 55-55.
https://hdl.handle.net/21.15107/rcub_rimi_1437

Ivanov M, Kovačević S, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Nešović Ostojić J. Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat. in 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book. 2022;:55-55.
https://hdl.handle.net/21.15107/rcub_rimi_1437 .

Ivanov, Milan, Kovačević, Sanjin, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović Ostojić, Jelena, "Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat" in 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book (2022):55-55,
https://hdl.handle.net/21.15107/rcub_rimi_1437 .

TY  - CONF
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Miloradović, Zoran
AU  - Brkić, Predrag
AU  - Jeremić, Rada
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1436
C3  - 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book
T1  - Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive
SP  - P-29
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1436
ER  - 

@conference{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Miloradović, Zoran and Brkić, Predrag and Jeremić, Rada and Karanović, Danijela and Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2022",
journal = "46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book",
title = "Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive",
pages = "P-29",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1436"
}

Kovačević, S., Ivanov, M., Životić, M., Miloradović, Z., Brkić, P., Jeremić, R., Karanović, D., Vajić, U. J., Mihailović-Stanojević, N., Jovović, Đ.,& Nešović Ostojić, J.. (2022). Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive. in 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book, P-29.
https://hdl.handle.net/21.15107/rcub_rimi_1436

Kovačević S, Ivanov M, Životić M, Miloradović Z, Brkić P, Jeremić R, Karanović D, Vajić UJ, Mihailović-Stanojević N, Jovović Đ, Nešović Ostojić J. Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive. in 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book. 2022;:P-29.
https://hdl.handle.net/21.15107/rcub_rimi_1436 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Miloradović, Zoran, Brkić, Predrag, Jeremić, Rada, Karanović, Danijela, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Jovović, Đurđica, Nešović Ostojić, Jelena, "Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive" in 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book (2022):P-29,
https://hdl.handle.net/21.15107/rcub_rimi_1436 .

TY  - JOUR
AU  - Vajić, Una Jovana
AU  - Živković, Jelena
AU  - Ivanov, Milan
AU  - Jovović, Đurđica
AU  - Šavikin, Katarina
AU  - Bugarski, Branko
AU  - Mihailović-Stanojević, Nevena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1269
AB  - The  aim  of  this  study  was  to  optimize  the  parameters  for  the  extraction  of  total  flavonoids  from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless  of  extraction  time,  aqueous  methanolicextracts  had  higher  total  flavonoid  content  than  did aqueous ethanolic extracts. So, full factorial design and response  surface  methodology (RSM)  were  em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol  content  in  the  solvent  influenced  significantly  total  flavonoid  content  and FRAP  (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous  methanol  and  67  min,  and  65%  aqueous  methanol  and  83  min  for  maximal FRAPantioxidantcapacity.  DPPH  (2,2-diphenyl-1-picrylhydrazyl)  antioxidant  capacity  was  not  significantly  affected  by extraction time or methanol percentage in the solvent.
PB  - Society of Chemists and Technologists of Macedonia and  Ss. Cyril and Methodius University in Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology
EP  - 128
IS  - 1
SP  - 119
VL  - 41
DO  - 10.20450/mjcce.2022.2238
ER  - 

@article{
author = "Vajić, Una Jovana and Živković, Jelena and Ivanov, Milan and Jovović, Đurđica and Šavikin, Katarina and Bugarski, Branko and Mihailović-Stanojević, Nevena",
year = "2022",
abstract = "The  aim  of  this  study  was  to  optimize  the  parameters  for  the  extraction  of  total  flavonoids  from stinging nettle leaf. Comparison of the effects of different solvents on total flavonoid content showed that, regardless  of  extraction  time,  aqueous  methanolicextracts  had  higher  total  flavonoid  content  than  did aqueous ethanolic extracts. So, full factorial design and response  surface  methodology (RSM)  were  em-ployed to estimate the effects of methanol content (50, 75and 100%) and extraction time (30, 60and 90 min) on the total flavonoid content and antioxidant capacities of the extracts. RSM analysis showed that methanol  content  in  the  solvent  influenced  significantly  total  flavonoid  content  and FRAP  (ferric-reducing antioxidant power) antioxidant capacity, while extraction time had no significant effect on either of these responses. Extraction parameters for maximal total flavonoid content were estimated to be 69% aqueous  methanol  and  67  min,  and  65%  aqueous  methanol  and  83  min  for  maximal FRAPantioxidantcapacity.  DPPH  (2,2-diphenyl-1-picrylhydrazyl)  antioxidant  capacity  was  not  significantly  affected  by extraction time or methanol percentage in the solvent.",
publisher = "Society of Chemists and Technologists of Macedonia and  Ss. Cyril and Methodius University in Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology",
pages = "128-119",
number = "1",
volume = "41",
doi = "10.20450/mjcce.2022.2238"
}

Vajić, U. J., Živković, J., Ivanov, M., Jovović, Đ., Šavikin, K., Bugarski, B.,& Mihailović-Stanojević, N.. (2022). Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering
Society of Chemists and Technologists of Macedonia and  Ss. Cyril and Methodius University in Skopje., 41(1), 119-128.
https://doi.org/10.20450/mjcce.2022.2238

Vajić UJ, Živković J, Ivanov M, Jovović Đ, Šavikin K, Bugarski B, Mihailović-Stanojević N. Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology. in Macedonian Journal of Chemistry and Chemical Engineering. 2022;41(1):119-128.
doi:10.20450/mjcce.2022.2238 .

Vajić, Una Jovana, Živković, Jelena, Ivanov, Milan, Jovović, Đurđica, Šavikin, Katarina, Bugarski, Branko, Mihailović-Stanojević, Nevena, "Optimization of the extraction of antioxidants from stinging nettle leaf using response surface methodology" in Macedonian Journal of Chemistry and Chemical Engineering, 41, no. 1 (2022):119-128,
https://doi.org/10.20450/mjcce.2022.2238 . .

TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Ostojić, Jelena Nešović
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1251
T2  - Journal of Hypertension
T2  - Journal of Hypertension
T1  - EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS
IS  - Suppl 1
SP  - e251
VL  - 40
DO  - 10.1097/01.hjh.0000838064.87501.71
ER  - 

@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Ostojić, Jelena Nešović",
year = "2022",
journal = "Journal of Hypertension, Journal of Hypertension",
title = "EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS",
number = "Suppl 1",
pages = "e251",
volume = "40",
doi = "10.1097/01.hjh.0000838064.87501.71"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Ostojić, J. N.. (2022). EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS. in Journal of Hypertension, 40(Suppl 1), e251.
https://doi.org/10.1097/01.hjh.0000838064.87501.71

Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Ostojić JN. EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS. in Journal of Hypertension. 2022;40(Suppl 1):e251.
doi:10.1097/01.hjh.0000838064.87501.71 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Ostojić, Jelena Nešović, "EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS" in Journal of Hypertension, 40, no. Suppl 1 (2022):e251,
https://doi.org/10.1097/01.hjh.0000838064.87501.71 . .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Jaćević, Vesna
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Milosavljević, Ivica
AU  - Milanović, Slađan
AU  - Mihailović-Stanojević, Nevena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1137
AB  - Hypertension is one of the most prevalent and powerful contributors of cardiovascular diseases. Malignant hypertension is a relatively rare but extremely severe form of hypertension accompanied with heart, brain, and renal impairment. Resveratrol, a recently described grape-derived, polyphenolic antioxidant molecule, has been proposed as an effective agent in the prevention of cardiovascular diseases. This study was designed to examine chronic resveratrol administration on blood pressure, oxidative stress, and inflammation, with special emphasis on cardiac structure and function in two models of experimental hypertension. The experiments were performed in spontaneously (SHRs) and malignantly hypertensive rats (MHRs). The chronic administration of resveratrol significantly decreased blood pressure in both spontaneously and malignant hypertensive animals. The resveratrol treatment ameliorated morphological changes in the heart tissue. The immunohistochemistry of the heart tissue after resveratrol treatment showed that both TGF-β and Bax were not present in the myocytes of SHRs and were present mainly in the myocytes of MHRs. Resveratrol suppressed lipid peroxidation and significantly improved oxidative status and release of NO. These results suggest that resveratrol prevents hypertrophic and apoptotic consequences induced by high blood pressure with more pronounced effects in malignant hypertension.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties
IS  - 9
SP  - 5006
VL  - 22
DO  - 10.3390/ijms22095006
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Jaćević, Vesna and Miloradović, Zoran and Jovović, Đurđica and Milosavljević, Ivica and Milanović, Slađan and Mihailović-Stanojević, Nevena",
year = "2021",
abstract = "Hypertension is one of the most prevalent and powerful contributors of cardiovascular diseases. Malignant hypertension is a relatively rare but extremely severe form of hypertension accompanied with heart, brain, and renal impairment. Resveratrol, a recently described grape-derived, polyphenolic antioxidant molecule, has been proposed as an effective agent in the prevention of cardiovascular diseases. This study was designed to examine chronic resveratrol administration on blood pressure, oxidative stress, and inflammation, with special emphasis on cardiac structure and function in two models of experimental hypertension. The experiments were performed in spontaneously (SHRs) and malignantly hypertensive rats (MHRs). The chronic administration of resveratrol significantly decreased blood pressure in both spontaneously and malignant hypertensive animals. The resveratrol treatment ameliorated morphological changes in the heart tissue. The immunohistochemistry of the heart tissue after resveratrol treatment showed that both TGF-β and Bax were not present in the myocytes of SHRs and were present mainly in the myocytes of MHRs. Resveratrol suppressed lipid peroxidation and significantly improved oxidative status and release of NO. These results suggest that resveratrol prevents hypertrophic and apoptotic consequences induced by high blood pressure with more pronounced effects in malignant hypertension.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties",
number = "9",
pages = "5006",
volume = "22",
doi = "10.3390/ijms22095006"
}

Grujić-Milanović, J., Jaćević, V., Miloradović, Z., Jovović, Đ., Milosavljević, I., Milanović, S.,& Mihailović-Stanojević, N.. (2021). Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties. in International Journal of Molecular Sciences
MDPI., 22(9), 5006.
https://doi.org/10.3390/ijms22095006

Grujić-Milanović J, Jaćević V, Miloradović Z, Jovović Đ, Milosavljević I, Milanović S, Mihailović-Stanojević N. Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties. in International Journal of Molecular Sciences. 2021;22(9):5006.
doi:10.3390/ijms22095006 .

Grujić-Milanović, Jelica, Jaćević, Vesna, Miloradović, Zoran, Jovović, Đurđica, Milosavljević, Ivica, Milanović, Slađan, Mihailović-Stanojević, Nevena, "Resveratrol Protects Cardiac Tissue in Experimental Malignant Hypertension Due to Antioxidant, Anti-Inflammatory, and Anti-Apoptotic Properties" in International Journal of Molecular Sciences, 22, no. 9 (2021):5006,
https://doi.org/10.3390/ijms22095006 . .

TY  - JOUR
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Vajić, Una Jovana
AU  - Grujić-Milanović, Jelica
AU  - Marković-Lipkovski, Jasmina
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/5
AB  - Olive (Olea europaea L.) leaf extract (OLE) possesses powerful antioxidant, antihyperlipidemic, and anti-inflammatory properties. The aim was to investigated the effects of OLE on the hyperlipidemia, antioxidant defense, heme oxygenase/biliverdin reductase (HO/BVR) pathway, inflammation, and fibrosis in spontaneously hypertensive rats with focal segmental glomerulosclerosis (FSGS, a progressive form of chronic kidney disease) induced by adriamycin (2 mg/kg, i.v., twice in a 21-day period). Daily treatment of OLE (80 mg/kg, p.o.) for 6 weeks suppressed protein oxidation and lipid peroxidation (p  lt  .01 and p  lt  .001, respectively), significantly increased antioxidant enzymes activities and normalized antioxidant capacity, leading to the improvement of antioxidant defense independently of the HO/BVR pathway. Furthermore, the values of triglycerides (p  lt  .01), total, and low-density lipoprotein cholesterol (p  lt  .05, both) were improved by OLE. OLE strongly prevented glomerulosclerosis, interstitial inflammation, and fibrosis (renal injury score, FSGS: 8 +/- 0.45 vs. FSGS+OLE: 4.20 +/- 1.07; p  lt  .01), as evidenced by normalized fibronectin content (p  lt  .001), suppressed interstitial inflammatory cells infiltration and collagen deposition, without changing cytokines expressions. OLE decreased blood pressure with a tendency to reduce urine albumin loss. These data suggest that OLE may be effective in slowing down the progression of FSGS.
PB  - Wiley, Hoboken
T2  - Phytotherapy Research
T1  - Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis
EP  - 1545
IS  - 3
SP  - 1534
VL  - 35
DO  - 10.1002/ptr.6920
ER  - 

@article{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Vajić, Una Jovana and Grujić-Milanović, Jelica and Marković-Lipkovski, Jasmina and Dekanski, Dragana and Jovović, Đurđica",
year = "2021",
abstract = "Olive (Olea europaea L.) leaf extract (OLE) possesses powerful antioxidant, antihyperlipidemic, and anti-inflammatory properties. The aim was to investigated the effects of OLE on the hyperlipidemia, antioxidant defense, heme oxygenase/biliverdin reductase (HO/BVR) pathway, inflammation, and fibrosis in spontaneously hypertensive rats with focal segmental glomerulosclerosis (FSGS, a progressive form of chronic kidney disease) induced by adriamycin (2 mg/kg, i.v., twice in a 21-day period). Daily treatment of OLE (80 mg/kg, p.o.) for 6 weeks suppressed protein oxidation and lipid peroxidation (p  lt  .01 and p  lt  .001, respectively), significantly increased antioxidant enzymes activities and normalized antioxidant capacity, leading to the improvement of antioxidant defense independently of the HO/BVR pathway. Furthermore, the values of triglycerides (p  lt  .01), total, and low-density lipoprotein cholesterol (p  lt  .05, both) were improved by OLE. OLE strongly prevented glomerulosclerosis, interstitial inflammation, and fibrosis (renal injury score, FSGS: 8 +/- 0.45 vs. FSGS+OLE: 4.20 +/- 1.07; p  lt  .01), as evidenced by normalized fibronectin content (p  lt  .001), suppressed interstitial inflammatory cells infiltration and collagen deposition, without changing cytokines expressions. OLE decreased blood pressure with a tendency to reduce urine albumin loss. These data suggest that OLE may be effective in slowing down the progression of FSGS.",
publisher = "Wiley, Hoboken",
journal = "Phytotherapy Research",
title = "Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis",
pages = "1545-1534",
number = "3",
volume = "35",
doi = "10.1002/ptr.6920"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Vajić, U. J., Grujić-Milanović, J., Marković-Lipkovski, J., Dekanski, D.,& Jovović, Đ.. (2021). Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis. in Phytotherapy Research
Wiley, Hoboken., 35(3), 1534-1545.
https://doi.org/10.1002/ptr.6920

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Vajić UJ, Grujić-Milanović J, Marković-Lipkovski J, Dekanski D, Jovović Đ. Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis. in Phytotherapy Research. 2021;35(3):1534-1545.
doi:10.1002/ptr.6920 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Vajić, Una Jovana, Grujić-Milanović, Jelica, Marković-Lipkovski, Jasmina, Dekanski, Dragana, Jovović, Đurđica, "Olive leaf extract attenuates adriamycin-induced focal segmental glomerulosclerosis in spontaneously hypertensive rats via suppression of oxidative stress, hyperlipidemia, and fibrosis" in Phytotherapy Research, 35, no. 3 (2021):1534-1545,
https://doi.org/10.1002/ptr.6920 . .

TY  - JOUR
AU  - Nešović-Ostojić, Jelena
AU  - Ivanov, Milan
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Kovacević, Sanjin
AU  - Brkić, Predrag
AU  - Životić, Maja
AU  - Vajić, Una Jovana
AU  - Jovović, Đurđica
AU  - Jeremić, Rada
AU  - Ljubojević-Holzer, Senka
AU  - Miloradović, Zoran
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1083
AB  - Renal ischemia and reperfusion (I/R) injury is the most common cause of acute kidney injury (AKI). Pathogenesis of postischemic AKI involves hemodynamic changes, oxidative stress, inflammation process, calcium ion overloading, apoptosis and necrosis. Up to date, therapeutic approaches to treat AKI are extremely limited. Thus, the aim of this study was to evaluate the effects of hyperbaric oxygen (HBO) preconditioning on citoprotective enzyme, heme oxygenase-1 (HO-1), pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins expression, in postischemic AKI induced in normotensive Wistar and spontaneously hypertensive rats (SHR). The animals were randomly divided into six experimental groups: SHAM-operated Wistar rats (W-SHAM), Wistar rats with induced postischemic AKI (W-AKI) and Wistar group with HBO preconditioning before AKI induction (W-AKI + HBO). On the other hand, SHR rats were also divided into same three groups: SHR-SHAM, SHR-AKI and SHR-AKI + HBO. We demonstrated that HBO preconditioning upregulated HO-1 and anti-apoptotic Bcl-2 protein expression, in both Wistar and SH rats. In addition, HBO preconditioning improved glomerular filtration rate, supporting by significant increase in creatinine, urea and phosphate clearances in both rat strains. Considering our results, we can also say that even in hypertensive conditions, we can expect protective effects of HBO preconditioning in experimental model of AKI.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury
IS  - 3
SP  - 1382
VL  - 22
DO  - 10.3390/ijms22031382
ER  - 

@article{
author = "Nešović-Ostojić, Jelena and Ivanov, Milan and Mihailović-Stanojević, Nevena and Karanović, Danijela and Kovacević, Sanjin and Brkić, Predrag and Životić, Maja and Vajić, Una Jovana and Jovović, Đurđica and Jeremić, Rada and Ljubojević-Holzer, Senka and Miloradović, Zoran",
year = "2021",
abstract = "Renal ischemia and reperfusion (I/R) injury is the most common cause of acute kidney injury (AKI). Pathogenesis of postischemic AKI involves hemodynamic changes, oxidative stress, inflammation process, calcium ion overloading, apoptosis and necrosis. Up to date, therapeutic approaches to treat AKI are extremely limited. Thus, the aim of this study was to evaluate the effects of hyperbaric oxygen (HBO) preconditioning on citoprotective enzyme, heme oxygenase-1 (HO-1), pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins expression, in postischemic AKI induced in normotensive Wistar and spontaneously hypertensive rats (SHR). The animals were randomly divided into six experimental groups: SHAM-operated Wistar rats (W-SHAM), Wistar rats with induced postischemic AKI (W-AKI) and Wistar group with HBO preconditioning before AKI induction (W-AKI + HBO). On the other hand, SHR rats were also divided into same three groups: SHR-SHAM, SHR-AKI and SHR-AKI + HBO. We demonstrated that HBO preconditioning upregulated HO-1 and anti-apoptotic Bcl-2 protein expression, in both Wistar and SH rats. In addition, HBO preconditioning improved glomerular filtration rate, supporting by significant increase in creatinine, urea and phosphate clearances in both rat strains. Considering our results, we can also say that even in hypertensive conditions, we can expect protective effects of HBO preconditioning in experimental model of AKI.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury",
number = "3",
pages = "1382",
volume = "22",
doi = "10.3390/ijms22031382"
}

Nešović-Ostojić, J., Ivanov, M., Mihailović-Stanojević, N., Karanović, D., Kovacević, S., Brkić, P., Životić, M., Vajić, U. J., Jovović, Đ., Jeremić, R., Ljubojević-Holzer, S.,& Miloradović, Z.. (2021). Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury. in International Journal of Molecular Sciences
MDPI, Basel., 22(3), 1382.
https://doi.org/10.3390/ijms22031382

Nešović-Ostojić J, Ivanov M, Mihailović-Stanojević N, Karanović D, Kovacević S, Brkić P, Životić M, Vajić UJ, Jovović Đ, Jeremić R, Ljubojević-Holzer S, Miloradović Z. Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury. in International Journal of Molecular Sciences. 2021;22(3):1382.
doi:10.3390/ijms22031382 .

Nešović-Ostojić, Jelena, Ivanov, Milan, Mihailović-Stanojević, Nevena, Karanović, Danijela, Kovacević, Sanjin, Brkić, Predrag, Životić, Maja, Vajić, Una Jovana, Jovović, Đurđica, Jeremić, Rada, Ljubojević-Holzer, Senka, Miloradović, Zoran, "Hyperbaric Oxygen Preconditioning Upregulates Heme OxyGenase-1 and Anti-Apoptotic Bcl-2 Protein Expression in Spontaneously Hypertensive Rats with Induced Postischemic Acute Kidney Injury" in International Journal of Molecular Sciences, 22, no. 3 (2021):1382,
https://doi.org/10.3390/ijms22031382 . .

TY  - JOUR
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Banjac, Vojislav V.
AU  - Vidosavljević, Strahinja
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Jovović, Đurđica
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1132
AB  - Lifestyle modifications such as increase in high-fat food consumption importantly increases the risks for cardiovascular disease. The principal objective of this study is to analyze effects of different high fat diet (HFD) sources on haemodynamic parameters, lipid and oxidative profile, myeloperoxidase activity, and markers of inflammation (IL-6/pentraxin-3). HFD containing 20% of fat, provided by lard (saturated) or soybean oil (unsaturated), as well as control diet were administering to three groups (L, SO and C). Food efficiency ratio and plasma lipids were significantly elevated in both HFD groups. However, only SO group showed an increase in systolic arterial pressure, oxidative stress index, myeloperoxidase activity, liver lipids as well as markers of inflammation: IL-6 and pentraxin-3 (PTX3). In summary, these results indicate inflammogenic potential of excessive soybean oil consumption in triggering liver damage.
PB  - Elsevier
T2  - Biomedicine & Pharmacotherapy
T1  - Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats
SP  - 111691
VL  - 139
DO  - 10.1016/j.biopha.2021.111691
ER  - 

@article{
author = "Grujić-Milanović, Jelica and Miloradović, Zoran and Mihailović-Stanojević, Nevena and Banjac, Vojislav V. and Vidosavljević, Strahinja and Ivanov, Milan and Karanović, Danijela and Vajić, Una Jovana and Jovović, Đurđica",
year = "2021",
abstract = "Lifestyle modifications such as increase in high-fat food consumption importantly increases the risks for cardiovascular disease. The principal objective of this study is to analyze effects of different high fat diet (HFD) sources on haemodynamic parameters, lipid and oxidative profile, myeloperoxidase activity, and markers of inflammation (IL-6/pentraxin-3). HFD containing 20% of fat, provided by lard (saturated) or soybean oil (unsaturated), as well as control diet were administering to three groups (L, SO and C). Food efficiency ratio and plasma lipids were significantly elevated in both HFD groups. However, only SO group showed an increase in systolic arterial pressure, oxidative stress index, myeloperoxidase activity, liver lipids as well as markers of inflammation: IL-6 and pentraxin-3 (PTX3). In summary, these results indicate inflammogenic potential of excessive soybean oil consumption in triggering liver damage.",
publisher = "Elsevier",
journal = "Biomedicine & Pharmacotherapy",
title = "Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats",
pages = "111691",
volume = "139",
doi = "10.1016/j.biopha.2021.111691"
}

Grujić-Milanović, J., Miloradović, Z., Mihailović-Stanojević, N., Banjac, V. V., Vidosavljević, S., Ivanov, M., Karanović, D., Vajić, U. J.,& Jovović, Đ.. (2021). Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats. in Biomedicine & Pharmacotherapy
Elsevier., 139, 111691.
https://doi.org/10.1016/j.biopha.2021.111691

Grujić-Milanović J, Miloradović Z, Mihailović-Stanojević N, Banjac VV, Vidosavljević S, Ivanov M, Karanović D, Vajić UJ, Jovović Đ. Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats. in Biomedicine & Pharmacotherapy. 2021;139:111691.
doi:10.1016/j.biopha.2021.111691 .

Grujić-Milanović, Jelica, Miloradović, Zoran, Mihailović-Stanojević, Nevena, Banjac, Vojislav V., Vidosavljević, Strahinja, Ivanov, Milan, Karanović, Danijela, Vajić, Una Jovana, Jovović, Đurđica, "Excesive consumption of unsaturated fatty acids leads to oxidative and inflammatory instability in Wistar rats" in Biomedicine & Pharmacotherapy, 139 (2021):111691,
https://doi.org/10.1016/j.biopha.2021.111691 . .

TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović-Ostojić, Jelena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1155
AB  - Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Antioxidants
T1  - Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
IS  - 8
SP  - 1163
VL  - 10
DO  - 10.3390/antiox10081163
ER  - 

@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović-Ostojić, Jelena",
year = "2021",
abstract = "Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Antioxidants",
title = "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats",
number = "8",
pages = "1163",
volume = "10",
doi = "10.3390/antiox10081163"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović-Ostojić, J.. (2021). Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants
Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 1163.
https://doi.org/10.3390/antiox10081163

Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Nešović-Ostojić J. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants. 2021;10(8):1163.
doi:10.3390/antiox10081163 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović-Ostojić, Jelena, "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats" in Antioxidants, 10, no. 8 (2021):1163,
https://doi.org/10.3390/antiox10081163 . .

TY  - JOUR
AU  - Kurćubić, Ivana
AU  - Vajić, Una Jovana
AU  - Cvijić, Sandra
AU  - Crevar-Sakač, Milkica
AU  - Bogavac-Stanojević, Nataša
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Đuriš, Jelena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1188
AB  - The objective of this study was to formulate extended-release mucoadhesive buccal tablets of propranolol hydrochloride in order to provide a prolonged absorption of propranolol hydrochloride from the buccal mucosa and to reduce presystemic metabolism and thus provide a better therapeutic effect. Besides, the aim was to perform comparative in vivo pharmacokinetic and hemodynamic studies of the developed extended-release (ER) propranolol hydrochloride 10 mg mucoadhesive buccal tablets and commercial immediate-release (IR) propranolol hydrochloride 10 mg tablets in spontaneously hypertensive rats. Formulation with 15% polyethylene oxide showed the highest degree of propranolol hydrochloride permeation, satisfactory mucoadhesiveness, and extended-release of propranolol hydrochloride, thus it was selected for further in vivo study. The pharmacokinetic study in rats showed the superiority of ER mucoadhesive buccal tablets over IR tablets in terms of propranolol hydrochloride absorption extent (AUC values: 70.32 ± 19.56 versus 31.69 ± 6.97 µg·h/mL), although lower maximum plasma propranolol hydrochloride concentration (Cmax) was achieved. However, no statistically significant difference was observed in Cmax between these treatments. The hemodynamic study showed that ER mucoadhesive buccal tablets provide a more pronounced decrease primarily in heart rate, but also in systolic and diastolic arterial pressure, as well as a longer heart rate reduction compared to IR tablets.
PB  - Elsevier
T2  - International Journal of Pharmaceutics
T1  - Mucoadhesive buccal tablets with propranolol hydrochloride: Formulation development and in vivo performances in experimental essential hypertension
SP  - 121266
VL  - 610
DO  - 10.1016/j.ijpharm.2021.121266
ER  - 

@article{
author = "Kurćubić, Ivana and Vajić, Una Jovana and Cvijić, Sandra and Crevar-Sakač, Milkica and Bogavac-Stanojević, Nataša and Miloradović, Zoran and Mihailović-Stanojević, Nevena and Ivanov, Milan and Karanović, Danijela and Jovović, Đurđica and Đuriš, Jelena",
year = "2021",
abstract = "The objective of this study was to formulate extended-release mucoadhesive buccal tablets of propranolol hydrochloride in order to provide a prolonged absorption of propranolol hydrochloride from the buccal mucosa and to reduce presystemic metabolism and thus provide a better therapeutic effect. Besides, the aim was to perform comparative in vivo pharmacokinetic and hemodynamic studies of the developed extended-release (ER) propranolol hydrochloride 10 mg mucoadhesive buccal tablets and commercial immediate-release (IR) propranolol hydrochloride 10 mg tablets in spontaneously hypertensive rats. Formulation with 15% polyethylene oxide showed the highest degree of propranolol hydrochloride permeation, satisfactory mucoadhesiveness, and extended-release of propranolol hydrochloride, thus it was selected for further in vivo study. The pharmacokinetic study in rats showed the superiority of ER mucoadhesive buccal tablets over IR tablets in terms of propranolol hydrochloride absorption extent (AUC values: 70.32 ± 19.56 versus 31.69 ± 6.97 µg·h/mL), although lower maximum plasma propranolol hydrochloride concentration (Cmax) was achieved. However, no statistically significant difference was observed in Cmax between these treatments. The hemodynamic study showed that ER mucoadhesive buccal tablets provide a more pronounced decrease primarily in heart rate, but also in systolic and diastolic arterial pressure, as well as a longer heart rate reduction compared to IR tablets.",
publisher = "Elsevier",
journal = "International Journal of Pharmaceutics",
title = "Mucoadhesive buccal tablets with propranolol hydrochloride: Formulation development and in vivo performances in experimental essential hypertension",
pages = "121266",
volume = "610",
doi = "10.1016/j.ijpharm.2021.121266"
}

Kurćubić, I., Vajić, U. J., Cvijić, S., Crevar-Sakač, M., Bogavac-Stanojević, N., Miloradović, Z., Mihailović-Stanojević, N., Ivanov, M., Karanović, D., Jovović, Đ.,& Đuriš, J.. (2021). Mucoadhesive buccal tablets with propranolol hydrochloride: Formulation development and in vivo performances in experimental essential hypertension. in International Journal of Pharmaceutics
Elsevier., 610, 121266.
https://doi.org/10.1016/j.ijpharm.2021.121266

Kurćubić I, Vajić UJ, Cvijić S, Crevar-Sakač M, Bogavac-Stanojević N, Miloradović Z, Mihailović-Stanojević N, Ivanov M, Karanović D, Jovović Đ, Đuriš J. Mucoadhesive buccal tablets with propranolol hydrochloride: Formulation development and in vivo performances in experimental essential hypertension. in International Journal of Pharmaceutics. 2021;610:121266.
doi:10.1016/j.ijpharm.2021.121266 .

Kurćubić, Ivana, Vajić, Una Jovana, Cvijić, Sandra, Crevar-Sakač, Milkica, Bogavac-Stanojević, Nataša, Miloradović, Zoran, Mihailović-Stanojević, Nevena, Ivanov, Milan, Karanović, Danijela, Jovović, Đurđica, Đuriš, Jelena, "Mucoadhesive buccal tablets with propranolol hydrochloride: Formulation development and in vivo performances in experimental essential hypertension" in International Journal of Pharmaceutics, 610 (2021):121266,
https://doi.org/10.1016/j.ijpharm.2021.121266 . .

TY  - JOUR
AU  - Bruić, Marija
AU  - Grujić-Milanović, Jelica
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Živković, Lada
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Spremo-Potparević, Biljana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1164
AB  - Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.
PB  - Elsevier
T2  - The International Journal of Biochemistry & Cell Biology
T1  - DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats
SP  - 106088
VL  - 141
DO  - 10.1016/j.biocel.2021.106088
ER  - 

@article{
author = "Bruić, Marija and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Živković, Lada and Mihailović-Stanojević, Nevena and Karanović, Danijela and Spremo-Potparević, Biljana",
year = "2021",
abstract = "Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.",
publisher = "Elsevier",
journal = "The International Journal of Biochemistry & Cell Biology",
title = "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats",
pages = "106088",
volume = "141",
doi = "10.1016/j.biocel.2021.106088"
}

Bruić, M., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Živković, L., Mihailović-Stanojević, N., Karanović, D.,& Spremo-Potparević, B.. (2021). DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in The International Journal of Biochemistry & Cell Biology
Elsevier., 141, 106088.
https://doi.org/10.1016/j.biocel.2021.106088

Bruić M, Grujić-Milanović J, Miloradović Z, Jovović Đ, Živković L, Mihailović-Stanojević N, Karanović D, Spremo-Potparević B. DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in The International Journal of Biochemistry & Cell Biology. 2021;141:106088.
doi:10.1016/j.biocel.2021.106088 .

Bruić, Marija, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Živković, Lada, Mihailović-Stanojević, Nevena, Karanović, Danijela, Spremo-Potparević, Biljana, "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats" in The International Journal of Biochemistry & Cell Biology, 141 (2021):106088,
https://doi.org/10.1016/j.biocel.2021.106088 . .

TY  - CONF
AU  - Karanović, Danijela
AU  - Mihailović-Stanojević, Nevena
AU  - Miloradović, Zoran
AU  - Ivanov, Milan
AU  - Grujić-Milanović, Jelica
AU  - Vajić, Una Jovana
AU  - Dekanski, Dragana
AU  - Jovović, Đurđica
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1048
PB  - Oxford Univ Press, Oxford
C3  - Nephrology Dialysis Transplantation
T1  - Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis
EP  - 1242
SP  - 1242
VL  - 35
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1048
ER  - 

@conference{
author = "Karanović, Danijela and Mihailović-Stanojević, Nevena and Miloradović, Zoran and Ivanov, Milan and Grujić-Milanović, Jelica and Vajić, Una Jovana and Dekanski, Dragana and Jovović, Đurđica",
year = "2020",
publisher = "Oxford Univ Press, Oxford",
journal = "Nephrology Dialysis Transplantation",
title = "Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis",
pages = "1242-1242",
volume = "35",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1048"
}

Karanović, D., Mihailović-Stanojević, N., Miloradović, Z., Ivanov, M., Grujić-Milanović, J., Vajić, U. J., Dekanski, D.,& Jovović, Đ.. (2020). Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis. in Nephrology Dialysis Transplantation
Oxford Univ Press, Oxford., 35, 1242-1242.
https://hdl.handle.net/21.15107/rcub_rimi_1048

Karanović D, Mihailović-Stanojević N, Miloradović Z, Ivanov M, Grujić-Milanović J, Vajić UJ, Dekanski D, Jovović Đ. Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis. in Nephrology Dialysis Transplantation. 2020;35:1242-1242.
https://hdl.handle.net/21.15107/rcub_rimi_1048 .

Karanović, Danijela, Mihailović-Stanojević, Nevena, Miloradović, Zoran, Ivanov, Milan, Grujić-Milanović, Jelica, Vajić, Una Jovana, Dekanski, Dragana, Jovović, Đurđica, "Olive leaf extract improves renal antioxidant defense without altering the heme oxygenase-1/biliverdin reductase pathway in hypertensive rats with focal segmental glomerulosclerosis" in Nephrology Dialysis Transplantation, 35 (2020):1242-1242,
https://hdl.handle.net/21.15107/rcub_rimi_1048 .

TY  - CONF
AU  - Ivanov, Milan
AU  - Miloradović, Zoran
AU  - Mihailović-Stanojević, Nevena
AU  - Jovović, Đurđica
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Jeremić, Rada
AU  - Đelić, Marina
AU  - Brkić, Predrag
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1049
PB  - Oxford Univ Press, Oxford
C3  - Nephrology Dialysis Transplantation
T1  - Hyperbaric oxygen preconditioning increases heme oxygenase 1 expression in kidney tissue and improves kidney function in spontaneously hypertensive rats with ischemic acute kidney injury
EP  - 877
SP  - 877
VL  - 35
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1049
ER  - 

@conference{
author = "Ivanov, Milan and Miloradović, Zoran and Mihailović-Stanojević, Nevena and Jovović, Đurđica and Karanović, Danijela and Vajić, Una Jovana and Jeremić, Rada and Đelić, Marina and Brkić, Predrag",
year = "2020",
publisher = "Oxford Univ Press, Oxford",
journal = "Nephrology Dialysis Transplantation",
title = "Hyperbaric oxygen preconditioning increases heme oxygenase 1 expression in kidney tissue and improves kidney function in spontaneously hypertensive rats with ischemic acute kidney injury",
pages = "877-877",
volume = "35",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1049"
}

Ivanov, M., Miloradović, Z., Mihailović-Stanojević, N., Jovović, Đ., Karanović, D., Vajić, U. J., Jeremić, R., Đelić, M.,& Brkić, P.. (2020). Hyperbaric oxygen preconditioning increases heme oxygenase 1 expression in kidney tissue and improves kidney function in spontaneously hypertensive rats with ischemic acute kidney injury. in Nephrology Dialysis Transplantation
Oxford Univ Press, Oxford., 35, 877-877.
https://hdl.handle.net/21.15107/rcub_rimi_1049

Ivanov M, Miloradović Z, Mihailović-Stanojević N, Jovović Đ, Karanović D, Vajić UJ, Jeremić R, Đelić M, Brkić P. Hyperbaric oxygen preconditioning increases heme oxygenase 1 expression in kidney tissue and improves kidney function in spontaneously hypertensive rats with ischemic acute kidney injury. in Nephrology Dialysis Transplantation. 2020;35:877-877.
https://hdl.handle.net/21.15107/rcub_rimi_1049 .

Ivanov, Milan, Miloradović, Zoran, Mihailović-Stanojević, Nevena, Jovović, Đurđica, Karanović, Danijela, Vajić, Una Jovana, Jeremić, Rada, Đelić, Marina, Brkić, Predrag, "Hyperbaric oxygen preconditioning increases heme oxygenase 1 expression in kidney tissue and improves kidney function in spontaneously hypertensive rats with ischemic acute kidney injury" in Nephrology Dialysis Transplantation, 35 (2020):877-877,
https://hdl.handle.net/21.15107/rcub_rimi_1049 .