TY  - CONF
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Jeremić, Rada
AU  - Nešović Ostojić, Jelena
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1434
C3  - 47th EUBS Annual Scientific Meeting, 13-16 September Porto, Portugal - Abstract & Conference Book
T1  - Hyperbaric oxygen preconditioning in postischemic acute kidney injury: potential mechanisms of beneficial effect
EP  - 90
SP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1434
ER  - 

@conference{
author = "Kovačević, Sanjin and Ivanov, Milan and Karanović, Danijela and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Jeremić, Rada and Nešović Ostojić, Jelena",
year = "2023",
journal = "47th EUBS Annual Scientific Meeting, 13-16 September Porto, Portugal - Abstract & Conference Book",
title = "Hyperbaric oxygen preconditioning in postischemic acute kidney injury: potential mechanisms of beneficial effect",
pages = "90-90",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1434"
}

Kovačević, S., Ivanov, M., Karanović, D., Životić, M., Brkić, P., Miloradović, Z., Vajić, U. J., Mihailović-Stanojević, N., Jeremić, R.,& Nešović Ostojić, J.. (2023). Hyperbaric oxygen preconditioning in postischemic acute kidney injury: potential mechanisms of beneficial effect. in 47th EUBS Annual Scientific Meeting, 13-16 September Porto, Portugal - Abstract & Conference Book, 90-90.
https://hdl.handle.net/21.15107/rcub_rimi_1434

Kovačević S, Ivanov M, Karanović D, Životić M, Brkić P, Miloradović Z, Vajić UJ, Mihailović-Stanojević N, Jeremić R, Nešović Ostojić J. Hyperbaric oxygen preconditioning in postischemic acute kidney injury: potential mechanisms of beneficial effect. in 47th EUBS Annual Scientific Meeting, 13-16 September Porto, Portugal - Abstract & Conference Book. 2023;:90-90.
https://hdl.handle.net/21.15107/rcub_rimi_1434 .

Kovačević, Sanjin, Ivanov, Milan, Karanović, Danijela, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Jeremić, Rada, Nešović Ostojić, Jelena, "Hyperbaric oxygen preconditioning in postischemic acute kidney injury: potential mechanisms of beneficial effect" in 47th EUBS Annual Scientific Meeting, 13-16 September Porto, Portugal - Abstract & Conference Book (2023):90-90,
https://hdl.handle.net/21.15107/rcub_rimi_1434 .

TY  - CONF
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Brkić, Predrag
AU  - Nešović Ostojić, Jelena
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1379
PB  - Niš: Punta
C3  - Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
T1  - Hyperbaric oxygen preconditioning: Can it be a novel supportive method in postischemic acute kidney injury?
EP  - 152
SP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1379
ER  - 

@conference{
author = "Kovačević, Sanjin and Ivanov, Milan and Brkić, Predrag and Nešović Ostojić, Jelena",
year = "2023",
publisher = "Niš: Punta",
journal = "Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia",
title = "Hyperbaric oxygen preconditioning: Can it be a novel supportive method in postischemic acute kidney injury?",
pages = "152-151",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1379"
}

Kovačević, S., Ivanov, M., Brkić, P.,& Nešović Ostojić, J.. (2023). Hyperbaric oxygen preconditioning: Can it be a novel supportive method in postischemic acute kidney injury?. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia
Niš: Punta., 151-152.
https://hdl.handle.net/21.15107/rcub_rimi_1379

Kovačević S, Ivanov M, Brkić P, Nešović Ostojić J. Hyperbaric oxygen preconditioning: Can it be a novel supportive method in postischemic acute kidney injury?. in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia. 2023;:151-152.
https://hdl.handle.net/21.15107/rcub_rimi_1379 .

Kovačević, Sanjin, Ivanov, Milan, Brkić, Predrag, Nešović Ostojić, Jelena, "Hyperbaric oxygen preconditioning: Can it be a novel supportive method in postischemic acute kidney injury?" in Cardionephrology : 6th International Congress of Cardionephrology - KARNEF 2023, May 29-30, 2023, Pirot, Serbia (2023):151-152,
https://hdl.handle.net/21.15107/rcub_rimi_1379 .

TY  - JOUR
AU  - Nešović-Ostojić, Jelena
AU  - Životić, Maja
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Brkić, Predrag
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Radojević-Škodrić, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1295
AB  - Ischemia–reperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variants’ (H2AX, γH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemia–reperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of γH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of γH2AX. In addition, we observed that different structures dominated when γH2AX and H2AXY142ph expression levels were compared. γH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acid–Schiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition.
T2  - International Journal of Molecular Sciences
T1  - Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury
IS  - 9
SP  - 8085
VL  - 24
DO  - 10.3390/ijms24098085
ER  - 

@article{
author = "Nešović-Ostojić, Jelena and Životić, Maja and Kovačević, Sanjin and Ivanov, Milan and Brkić, Predrag and Mihailović-Stanojević, Nevena and Karanović, Danijela and Vajić, Una Jovana and Miloradović, Zoran and Jovović, Đurđica and Radojević-Škodrić, Sanja",
year = "2023",
abstract = "Ischemia–reperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variants’ (H2AX, γH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemia–reperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of γH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of γH2AX. In addition, we observed that different structures dominated when γH2AX and H2AXY142ph expression levels were compared. γH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acid–Schiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition.",
journal = "International Journal of Molecular Sciences",
title = "Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury",
number = "9",
pages = "8085",
volume = "24",
doi = "10.3390/ijms24098085"
}

Nešović-Ostojić, J., Životić, M., Kovačević, S., Ivanov, M., Brkić, P., Mihailović-Stanojević, N., Karanović, D., Vajić, U. J., Miloradović, Z., Jovović, Đ.,& Radojević-Škodrić, S.. (2023). Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury. in International Journal of Molecular Sciences, 24(9), 8085.
https://doi.org/10.3390/ijms24098085

Nešović-Ostojić J, Životić M, Kovačević S, Ivanov M, Brkić P, Mihailović-Stanojević N, Karanović D, Vajić UJ, Miloradović Z, Jovović Đ, Radojević-Škodrić S. Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury. in International Journal of Molecular Sciences. 2023;24(9):8085.
doi:10.3390/ijms24098085 .

Nešović-Ostojić, Jelena, Životić, Maja, Kovačević, Sanjin, Ivanov, Milan, Brkić, Predrag, Mihailović-Stanojević, Nevena, Karanović, Danijela, Vajić, Una Jovana, Miloradović, Zoran, Jovović, Đurđica, Radojević-Škodrić, Sanja, "Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury" in International Journal of Molecular Sciences, 24, no. 9 (2023):8085,
https://doi.org/10.3390/ijms24098085 . .

TY  - CONF
AU  - Ivanov, Milan
AU  - Kovačević, Sanjin
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1437
AB  - Kidney disease represents a serious global health problem. Free radicals and prooxidants produced during ischemic acute kidney injury (AKI) may further aggravate the course of the disease and play a role in the pathogenesis of subsequent complications. The aims of our study were to examine the importance of NADPH oxidase blockade and to determine the effect of hyperbaric oxygen preconditioning on the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. NADPH oxidase blockade was performed by Apocynin (40 mg/kg body weigh)t, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning,with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, NADPH oxidase blockade and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
PB  - European Section of the International Academy of Cardiovascular Sciences
C3  - 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book
T1  - Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat
EP  - 55
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1437
ER  - 

@conference{
author = "Ivanov, Milan and Kovačević, Sanjin and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2022",
abstract = "Kidney disease represents a serious global health problem. Free radicals and prooxidants produced during ischemic acute kidney injury (AKI) may further aggravate the course of the disease and play a role in the pathogenesis of subsequent complications. The aims of our study were to examine the importance of NADPH oxidase blockade and to determine the effect of hyperbaric oxygen preconditioning on the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. NADPH oxidase blockade was performed by Apocynin (40 mg/kg body weigh)t, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning,with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, NADPH oxidase blockade and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.",
publisher = "European Section of the International Academy of Cardiovascular Sciences",
journal = "8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book",
title = "Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat",
pages = "55-55",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1437"
}

Ivanov, M., Kovačević, S., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović Ostojić, J.. (2022). Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat. in 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book
European Section of the International Academy of Cardiovascular Sciences., 55-55.
https://hdl.handle.net/21.15107/rcub_rimi_1437

Ivanov M, Kovačević S, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Nešović Ostojić J. Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat. in 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book. 2022;:55-55.
https://hdl.handle.net/21.15107/rcub_rimi_1437 .

Ivanov, Milan, Kovačević, Sanjin, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović Ostojić, Jelena, "Effects of NADPH oxidase blockade and hyperbaric oxygen preconditioning on 4-HNE, NGAL, and HO-1 tissue expression in postischemic acute kidney injury induced in spontaneously hypertensive rat" in 8th European Section Meeting of the International Academy of Cardiovascular Sciences (IACS) September 28-October 1, 2022 Szeged, Hungary - Programme and Abstract Book (2022):55-55,
https://hdl.handle.net/21.15107/rcub_rimi_1437 .

TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Ostojić, Jelena Nešović
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1251
T2  - Journal of Hypertension
T2  - Journal of Hypertension
T1  - EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS
IS  - Suppl 1
SP  - e251
VL  - 40
DO  - 10.1097/01.hjh.0000838064.87501.71
ER  - 

@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Ostojić, Jelena Nešović",
year = "2022",
journal = "Journal of Hypertension, Journal of Hypertension",
title = "EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS",
number = "Suppl 1",
pages = "e251",
volume = "40",
doi = "10.1097/01.hjh.0000838064.87501.71"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Ostojić, J. N.. (2022). EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS. in Journal of Hypertension, 40(Suppl 1), e251.
https://doi.org/10.1097/01.hjh.0000838064.87501.71

Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Ostojić JN. EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS. in Journal of Hypertension. 2022;40(Suppl 1):e251.
doi:10.1097/01.hjh.0000838064.87501.71 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Ostojić, Jelena Nešović, "EFFECTS OF APOCYNIN TREATMENT AND HBO PRECONDITIONING ON IMMUNOHISTOCHEMICAL 4-HNE, HIF1 ALPHA, HIF2 ALPHA AND NGAL TISSUE EXPRESSION IN POSTISCEMIC ACUTE KIDNEY INJURY IN SHR RATS" in Journal of Hypertension, 40, no. Suppl 1 (2022):e251,
https://doi.org/10.1097/01.hjh.0000838064.87501.71 . .

TY  - JOUR
AU  - Životić, Maja
AU  - Dunđerović, Duško
AU  - Naumović, Radomir
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Nikolić, Gorana
AU  - Marković-Lipkovski, Jasmina
AU  - Radojević-Škodrić, Sanja
AU  - Nešović-Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1277
AB  - Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed. Presence, location and extent of PAX8 expression were analyzed among 31 human kidney samples of AKI (25 autopsy cases, 5 kidney biopsies with unknown etiology and 1 AKI with confirmed myoglobin cast nephropathy), as well as in animals with induced postischemic AKI. Additionally, expression pattern was analyzed in 20 kidney biopsy samples of CKD. Our study demonstrates that various kidney diseases with chronic disease course that results in the formation of tubular atrophy and interstitial fibrosis, lead to PAX8 expression in the nuclei of proximal tubules. Furthermore, patients with PAX8 detected within the damaged proximal tubuli would be carefully monitored, since deterioration in kidney function was observed during follow-up. We also showed that myoglobin provoked acute kidney injury followed with large extent of renal damage, was associated with strong nuclear expression of PAX8 in proximal tubular cells. These results were supported and followed by data obtained in experimental model of induced postischemic acute kidney injury. Considering these findings, we can assume that PAX8 protein might be involved in regeneration process and recovery after acute kidney injury. Thus, accordingly, all investigation concerning PAX8 immunolabeling should be performed on biopsy samples of the living individuals.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Diagnostics
T1  - Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
IS  - 9
SP  - 2036
VL  - 12
DO  - 10.3390/diagnostics12092036
ER  - 

@article{
author = "Životić, Maja and Dunđerović, Duško and Naumović, Radomir and Kovačević, Sanjin and Ivanov, Milan and Karanović, Danijela and Nikolić, Gorana and Marković-Lipkovski, Jasmina and Radojević-Škodrić, Sanja and Nešović-Ostojić, Jelena",
year = "2022",
abstract = "Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed. Presence, location and extent of PAX8 expression were analyzed among 31 human kidney samples of AKI (25 autopsy cases, 5 kidney biopsies with unknown etiology and 1 AKI with confirmed myoglobin cast nephropathy), as well as in animals with induced postischemic AKI. Additionally, expression pattern was analyzed in 20 kidney biopsy samples of CKD. Our study demonstrates that various kidney diseases with chronic disease course that results in the formation of tubular atrophy and interstitial fibrosis, lead to PAX8 expression in the nuclei of proximal tubules. Furthermore, patients with PAX8 detected within the damaged proximal tubuli would be carefully monitored, since deterioration in kidney function was observed during follow-up. We also showed that myoglobin provoked acute kidney injury followed with large extent of renal damage, was associated with strong nuclear expression of PAX8 in proximal tubular cells. These results were supported and followed by data obtained in experimental model of induced postischemic acute kidney injury. Considering these findings, we can assume that PAX8 protein might be involved in regeneration process and recovery after acute kidney injury. Thus, accordingly, all investigation concerning PAX8 immunolabeling should be performed on biopsy samples of the living individuals.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Diagnostics",
title = "Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases",
number = "9",
pages = "2036",
volume = "12",
doi = "10.3390/diagnostics12092036"
}

Životić, M., Dunđerović, D., Naumović, R., Kovačević, S., Ivanov, M., Karanović, D., Nikolić, G., Marković-Lipkovski, J., Radojević-Škodrić, S.,& Nešović-Ostojić, J.. (2022). Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases. in Diagnostics
Multidisciplinary Digital Publishing Institute (MDPI)., 12(9), 2036.
https://doi.org/10.3390/diagnostics12092036

Životić M, Dunđerović D, Naumović R, Kovačević S, Ivanov M, Karanović D, Nikolić G, Marković-Lipkovski J, Radojević-Škodrić S, Nešović-Ostojić J. Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases. in Diagnostics. 2022;12(9):2036.
doi:10.3390/diagnostics12092036 .

Životić, Maja, Dunđerović, Duško, Naumović, Radomir, Kovačević, Sanjin, Ivanov, Milan, Karanović, Danijela, Nikolić, Gorana, Marković-Lipkovski, Jasmina, Radojević-Škodrić, Sanja, Nešović-Ostojić, Jelena, "Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases" in Diagnostics, 12, no. 9 (2022):2036,
https://doi.org/10.3390/diagnostics12092036 . .

TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović-Ostojić, Jelena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1155
AB  - Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Antioxidants
T1  - Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
IS  - 8
SP  - 1163
VL  - 10
DO  - 10.3390/antiox10081163
ER  - 

@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović-Ostojić, Jelena",
year = "2021",
abstract = "Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Antioxidants",
title = "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats",
number = "8",
pages = "1163",
volume = "10",
doi = "10.3390/antiox10081163"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović-Ostojić, J.. (2021). Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants
Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 1163.
https://doi.org/10.3390/antiox10081163

Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Nešović-Ostojić J. Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants. 2021;10(8):1163.
doi:10.3390/antiox10081163 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović-Ostojić, Jelena, "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats" in Antioxidants, 10, no. 8 (2021):1163,
https://doi.org/10.3390/antiox10081163 . .