TY  - JOUR
AU  - Nešović-Ostojić, Jelena
AU  - Životić, Maja
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Brkić, Predrag
AU  - Mihailović-Stanojević, Nevena
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Miloradović, Zoran
AU  - Jovović, Đurđica
AU  - Radojević-Škodrić, Sanja
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1295
AB  - Ischemia–reperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variants’ (H2AX, γH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemia–reperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of γH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of γH2AX. In addition, we observed that different structures dominated when γH2AX and H2AXY142ph expression levels were compared. γH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acid–Schiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition.
T2  - International Journal of Molecular Sciences
T1  - Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury
IS  - 9
SP  - 8085
VL  - 24
DO  - 10.3390/ijms24098085
ER  - 

@article{
author = "Nešović-Ostojić, Jelena and Životić, Maja and Kovačević, Sanjin and Ivanov, Milan and Brkić, Predrag and Mihailović-Stanojević, Nevena and Karanović, Danijela and Vajić, Una Jovana and Miloradović, Zoran and Jovović, Đurđica and Radojević-Škodrić, Sanja",
year = "2023",
abstract = "Ischemia–reperfusion injury (IRI) is a frequent cause of AKI, resulting in vasoconstriction, cellular dysfunction, inflammation and the induction of oxidative stress. DNA damage, including physical DNA strand breaks, is also a potential consequence of renal IRI. The histone H2A variants, primary H2AX and H2AZ participate in DNA damage response pathways to promote genome stability. The aim of this study was to evaluate the immunohistochemical pattern of histone H2A variants’ (H2AX, γH2AX(S139), H2AXY142ph and H2AZ) expression in an experimental model of ischemia–reperfusion-induced acute kidney injury in spontaneously hypertensive rats. Comparing the immunohistochemical nuclear expression of γH2AX(S139) and H2AXY142ph in AKI, we observed that there is an inverse ratio of these two histone H2AX variants. If we follow different regions from the subcapsular structures to the medulla, there is an increasing extent gradient in the nuclear expression of H2AXY142ph, accompanied by a decreasing nuclear expression of γH2AX. In addition, we observed that different structures dominated when γH2AX and H2AXY142ph expression levels were compared. γH2AX was expressed only in the proximal tubule, with the exception of when they were dilated. In the medulla, H2AXY142ph is predominantly expressed in the loop of Henle and the collecting ducts. Our results show moderate sporadic nuclear H2AZ expression mainly in the cells of the distal tubules and the collecting ducts that were surrounded by dilated tubules with PAS (periodic acid–Schiff stain)-positive casts. These findings may indicate the degree of DNA damage, followed by postischemic AKI, with potential clinical and prognostic implications regarding this condition.",
journal = "International Journal of Molecular Sciences",
title = "Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury",
number = "9",
pages = "8085",
volume = "24",
doi = "10.3390/ijms24098085"
}

Nešović-Ostojić, J., Životić, M., Kovačević, S., Ivanov, M., Brkić, P., Mihailović-Stanojević, N., Karanović, D., Vajić, U. J., Miloradović, Z., Jovović, Đ.,& Radojević-Škodrić, S.. (2023). Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury. in International Journal of Molecular Sciences, 24(9), 8085.
https://doi.org/10.3390/ijms24098085

Nešović-Ostojić J, Životić M, Kovačević S, Ivanov M, Brkić P, Mihailović-Stanojević N, Karanović D, Vajić UJ, Miloradović Z, Jovović Đ, Radojević-Škodrić S. Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury. in International Journal of Molecular Sciences. 2023;24(9):8085.
doi:10.3390/ijms24098085 .

Nešović-Ostojić, Jelena, Životić, Maja, Kovačević, Sanjin, Ivanov, Milan, Brkić, Predrag, Mihailović-Stanojević, Nevena, Karanović, Danijela, Vajić, Una Jovana, Miloradović, Zoran, Jovović, Đurđica, Radojević-Škodrić, Sanja, "Immunohistochemical Pattern of Histone H2A Variant Expression in an Experimental Model of Ischemia–Reperfusion-Induced Acute Kidney Injury" in International Journal of Molecular Sciences, 24, no. 9 (2023):8085,
https://doi.org/10.3390/ijms24098085 . .

TY  - CONF
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1440
AB  - Objective: 
Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). 4-hydroxynonenal (4-HNE) is a product of lipid peroxidation and may serve as non-invasive biomarker of oxidative stress while neutrophil gelatinase-associated lipocalin (NGAL) is a biomarker of AKI. Hypoxia-inducible factors (HIFs) are the “master’’ transcription factors responsible for gene expression in hypoxia, which play an important role in kidney injury and repair by regulating HIF target genes, including microRNAs.
Design and method: 
The study was conducted on male SHR rats, randomly divided into following groups: sham-operated group (SHAM) AKI control group, AKI group with apocynin treatment (AKI+APO), group with HBO preconditioning before AKI induction (AKI+HBO) and group with HBO preconditioning before and apocynin treatment after AKI induction (AKI+APO+HBO). HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24 h before AKI induction. Apocynin, NADPH oxidase inhibitor was injected 5 minutes before reperfusion.
Results: 
AKI induction significantly decreased creatinine (CCr), urea (CU) and phosphate (CPhos) clearances when compared to SHAM group. Remarkable increase in CCr, CU, CPhos levels were observed in all treated groups (AKI+APO, AKI+HBO, AKI+APO+HBO) in comparison to AKI group. Sham – operated rats did not express 4-HNE and NGAL in any parenchymal structure. AKI induced abundant and strong glomerular expression of 4-HNE along with expression in interstitial compartment. All treatments (AKI+APO, AKI+HBO, AKI+APO+HBO) significantly decreased 4-HNE expression both in glomeruli and interstitium. Also, AKI stimulated widespread NGAL expression in renal epithelial tubular cells with fine granular appearance on the apical surface of the cells, affecting entire circumference of tubular cross-sectioning, which was decreased in all treated groups. In SHAM group, the expression of HIF1 alpha and HIF2 alpha was focal with weak intensity in glomeruli and interstitium. In AKI group, the expression was diffuse and strong, with remarkable improvement in all treated groups.
Conclusions: 
Considering our results, we can conclude that apocynin treatment, as well as HBO preconditioning may have protective effects in postischemic acute kidney injury induced in spontaneously hypertensive rats.
PB  - Wolters Kluwer Health, Inc.
C3  - Journal of Hypertension
T1  - Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats
IS  - Suppl 1
SP  - e251
VL  - 40
DO  - 10.1097/01.hjh.0000838064.87501.71
ER  - 

@conference{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2022",
abstract = "Objective: 
Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). 4-hydroxynonenal (4-HNE) is a product of lipid peroxidation and may serve as non-invasive biomarker of oxidative stress while neutrophil gelatinase-associated lipocalin (NGAL) is a biomarker of AKI. Hypoxia-inducible factors (HIFs) are the “master’’ transcription factors responsible for gene expression in hypoxia, which play an important role in kidney injury and repair by regulating HIF target genes, including microRNAs.
Design and method: 
The study was conducted on male SHR rats, randomly divided into following groups: sham-operated group (SHAM) AKI control group, AKI group with apocynin treatment (AKI+APO), group with HBO preconditioning before AKI induction (AKI+HBO) and group with HBO preconditioning before and apocynin treatment after AKI induction (AKI+APO+HBO). HBO preconditioning was performed by exposing to pure oxygen (2.026 bar) twice a day for two consecutive days for 60 minutes, and 24 h before AKI induction. Apocynin, NADPH oxidase inhibitor was injected 5 minutes before reperfusion.
Results: 
AKI induction significantly decreased creatinine (CCr), urea (CU) and phosphate (CPhos) clearances when compared to SHAM group. Remarkable increase in CCr, CU, CPhos levels were observed in all treated groups (AKI+APO, AKI+HBO, AKI+APO+HBO) in comparison to AKI group. Sham – operated rats did not express 4-HNE and NGAL in any parenchymal structure. AKI induced abundant and strong glomerular expression of 4-HNE along with expression in interstitial compartment. All treatments (AKI+APO, AKI+HBO, AKI+APO+HBO) significantly decreased 4-HNE expression both in glomeruli and interstitium. Also, AKI stimulated widespread NGAL expression in renal epithelial tubular cells with fine granular appearance on the apical surface of the cells, affecting entire circumference of tubular cross-sectioning, which was decreased in all treated groups. In SHAM group, the expression of HIF1 alpha and HIF2 alpha was focal with weak intensity in glomeruli and interstitium. In AKI group, the expression was diffuse and strong, with remarkable improvement in all treated groups.
Conclusions: 
Considering our results, we can conclude that apocynin treatment, as well as HBO preconditioning may have protective effects in postischemic acute kidney injury induced in spontaneously hypertensive rats.",
publisher = "Wolters Kluwer Health, Inc.",
journal = "Journal of Hypertension",
title = "Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats",
number = "Suppl 1",
pages = "e251",
volume = "40",
doi = "10.1097/01.hjh.0000838064.87501.71"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović Ostojić, J.. (2022). Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats. in Journal of Hypertension
Wolters Kluwer Health, Inc.., 40(Suppl 1), e251.
https://doi.org/10.1097/01.hjh.0000838064.87501.71

Kovačević S, Ivanov M, Životić M, Brkić P, Miloradović Z, Jeremić R, Mihailović-Stanojević N, Vajić UJ, Karanović D, Jovović Đ, Nešović Ostojić J. Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats. in Journal of Hypertension. 2022;40(Suppl 1):e251.
doi:10.1097/01.hjh.0000838064.87501.71 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Brkić, Predrag, Miloradović, Zoran, Jeremić, Rada, Mihailović-Stanojević, Nevena, Vajić, Una Jovana, Karanović, Danijela, Jovović, Đurđica, Nešović Ostojić, Jelena, "Effects of apocynin treatment and HBO preconditioning on immunohistochemical 4-HNE, HIF alpha, HIF2 alpha and NGAL tissue expression in postiscemic acute kidney injury in SHR rats" in Journal of Hypertension, 40, no. Suppl 1 (2022):e251,
https://doi.org/10.1097/01.hjh.0000838064.87501.71 . .

TY  - CONF
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Miloradović, Zoran
AU  - Brkić, Predrag
AU  - Jeremić, Rada
AU  - Karanović, Danijela
AU  - Vajić, Una Jovana
AU  - Mihailović-Stanojević, Nevena
AU  - Jovović, Đurđica
AU  - Nešović Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1436
C3  - 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book
T1  - Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive
SP  - P-29
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1436
ER  - 

@conference{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Miloradović, Zoran and Brkić, Predrag and Jeremić, Rada and Karanović, Danijela and Vajić, Una Jovana and Mihailović-Stanojević, Nevena and Jovović, Đurđica and Nešović Ostojić, Jelena",
year = "2022",
journal = "46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book",
title = "Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive",
pages = "P-29",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1436"
}

Kovačević, S., Ivanov, M., Životić, M., Miloradović, Z., Brkić, P., Jeremić, R., Karanović, D., Vajić, U. J., Mihailović-Stanojević, N., Jovović, Đ.,& Nešović Ostojić, J.. (2022). Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive. in 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book, P-29.
https://hdl.handle.net/21.15107/rcub_rimi_1436

Kovačević S, Ivanov M, Životić M, Miloradović Z, Brkić P, Jeremić R, Karanović D, Vajić UJ, Mihailović-Stanojević N, Jovović Đ, Nešović Ostojić J. Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive. in 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book. 2022;:P-29.
https://hdl.handle.net/21.15107/rcub_rimi_1436 .

Kovačević, Sanjin, Ivanov, Milan, Životić, Maja, Miloradović, Zoran, Brkić, Predrag, Jeremić, Rada, Karanović, Danijela, Vajić, Una Jovana, Mihailović-Stanojević, Nevena, Jovović, Đurđica, Nešović Ostojić, Jelena, "Immunohistochemical expression of heme oxygenase-1, 4-hydroxynonenal and hypoxia inducible factors (1α, 1β, 2α) after HBO preconditioning in postischemic acute kidney injury induced in spontaneously hypertensive" in 46th EUBS Annual Scientific Meeting, 31.08.-03.09.2022. Prague, Czech Republic - Abstract & Conference Book (2022):P-29,
https://hdl.handle.net/21.15107/rcub_rimi_1436 .

TY  - JOUR
AU  - Životić, Maja
AU  - Dunđerović, Duško
AU  - Naumović, Radomir
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Karanović, Danijela
AU  - Nikolić, Gorana
AU  - Marković-Lipkovski, Jasmina
AU  - Radojević-Škodrić, Sanja
AU  - Nešović-Ostojić, Jelena
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1277
AB  - Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed. Presence, location and extent of PAX8 expression were analyzed among 31 human kidney samples of AKI (25 autopsy cases, 5 kidney biopsies with unknown etiology and 1 AKI with confirmed myoglobin cast nephropathy), as well as in animals with induced postischemic AKI. Additionally, expression pattern was analyzed in 20 kidney biopsy samples of CKD. Our study demonstrates that various kidney diseases with chronic disease course that results in the formation of tubular atrophy and interstitial fibrosis, lead to PAX8 expression in the nuclei of proximal tubules. Furthermore, patients with PAX8 detected within the damaged proximal tubuli would be carefully monitored, since deterioration in kidney function was observed during follow-up. We also showed that myoglobin provoked acute kidney injury followed with large extent of renal damage, was associated with strong nuclear expression of PAX8 in proximal tubular cells. These results were supported and followed by data obtained in experimental model of induced postischemic acute kidney injury. Considering these findings, we can assume that PAX8 protein might be involved in regeneration process and recovery after acute kidney injury. Thus, accordingly, all investigation concerning PAX8 immunolabeling should be performed on biopsy samples of the living individuals.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Diagnostics
T1  - Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases
IS  - 9
SP  - 2036
VL  - 12
DO  - 10.3390/diagnostics12092036
ER  - 

@article{
author = "Životić, Maja and Dunđerović, Duško and Naumović, Radomir and Kovačević, Sanjin and Ivanov, Milan and Karanović, Danijela and Nikolić, Gorana and Marković-Lipkovski, Jasmina and Radojević-Škodrić, Sanja and Nešović-Ostojić, Jelena",
year = "2022",
abstract = "Transcription factor PAX8, expressed during embryonic kidney development, has been previously detected in various kidney tumors. In order to investigate expression of PAX8 transcription factor in acute kidney injury (AKI) and chronic kidney diseases (CKD), immunohistochemical analysis was performed. Presence, location and extent of PAX8 expression were analyzed among 31 human kidney samples of AKI (25 autopsy cases, 5 kidney biopsies with unknown etiology and 1 AKI with confirmed myoglobin cast nephropathy), as well as in animals with induced postischemic AKI. Additionally, expression pattern was analyzed in 20 kidney biopsy samples of CKD. Our study demonstrates that various kidney diseases with chronic disease course that results in the formation of tubular atrophy and interstitial fibrosis, lead to PAX8 expression in the nuclei of proximal tubules. Furthermore, patients with PAX8 detected within the damaged proximal tubuli would be carefully monitored, since deterioration in kidney function was observed during follow-up. We also showed that myoglobin provoked acute kidney injury followed with large extent of renal damage, was associated with strong nuclear expression of PAX8 in proximal tubular cells. These results were supported and followed by data obtained in experimental model of induced postischemic acute kidney injury. Considering these findings, we can assume that PAX8 protein might be involved in regeneration process and recovery after acute kidney injury. Thus, accordingly, all investigation concerning PAX8 immunolabeling should be performed on biopsy samples of the living individuals.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Diagnostics",
title = "Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases",
number = "9",
pages = "2036",
volume = "12",
doi = "10.3390/diagnostics12092036"
}

Životić, M., Dunđerović, D., Naumović, R., Kovačević, S., Ivanov, M., Karanović, D., Nikolić, G., Marković-Lipkovski, J., Radojević-Škodrić, S.,& Nešović-Ostojić, J.. (2022). Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases. in Diagnostics
Multidisciplinary Digital Publishing Institute (MDPI)., 12(9), 2036.
https://doi.org/10.3390/diagnostics12092036

Životić M, Dunđerović D, Naumović R, Kovačević S, Ivanov M, Karanović D, Nikolić G, Marković-Lipkovski J, Radojević-Škodrić S, Nešović-Ostojić J. Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases. in Diagnostics. 2022;12(9):2036.
doi:10.3390/diagnostics12092036 .

Životić, Maja, Dunđerović, Duško, Naumović, Radomir, Kovačević, Sanjin, Ivanov, Milan, Karanović, Danijela, Nikolić, Gorana, Marković-Lipkovski, Jasmina, Radojević-Škodrić, Sanja, Nešović-Ostojić, Jelena, "Clinicopathological Relevance of PAX8 Expression Patterns in Acute Kidney Injury and Chronic Kidney Diseases" in Diagnostics, 12, no. 9 (2022):2036,
https://doi.org/10.3390/diagnostics12092036 . .

TY  - JOUR
AU  - Kovačević, Sanjin
AU  - Ivanov, Milan
AU  - Životić, Maja
AU  - Brkić, Predrag
AU  - Miloradović, Zoran
AU  - Jeremić, Rada
AU  - Mihailović-Stanojević, Nevena
AU  - Vajić, Una Jovana
AU  - Karanović, Danijela
AU  - Jovović, Đurđica
AU  - Nešović-Ostojić, Jelena
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1155
AB  - Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Antioxidants
T1  - Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats
IS  - 8
SP  - 1163
VL  - 10
DO  - 10.3390/antiox10081163
ER  - 

@article{
author = "Kovačević, Sanjin and Ivanov, Milan and Životić, Maja and Brkić, Predrag and Miloradović, Zoran and Jeremić, Rada and Mihailović-Stanojević, Nevena and Vajić, Una Jovana and Karanović, Danijela and Jovović, Đurđica and Nešović-Ostojić, Jelena",
year = "2021",
abstract = "Oxidative stress has been considered as a central aggravating factor in the development of postischemic acute kidney injury (AKI). The aim of this study was to perform the immunohistochemical analysis of 4-hydroxynonenal (4-HNE), neutrophil gelatinase-associated lipocalin (NGAL), and heme oxygenase-1 (HO-1) tissue expression after apocynin (APO) treatment and hyperbaric oxygenation (HBO) preconditioning, applied as single or combined protocol, in postischemic acute kidney injury induced in spontaneously hypertensive rats (SHR). Twenty-four hours before AKI induction, HBO preconditioning was carried out by exposing to pure oxygen (2.026 bar) twice a day, for 60 min in two consecutive days. Acute kidney injury was induced by removal of the right kidney while the left renal artery was occluded for 45 min by atraumatic clamp. Apocynin was applied in a dose of 40 mg/kg body weight, intravenously, 5 min before reperfusion. We showed increased 4-HNE renal expression in postischemic AKI compared to Sham-operated (SHAM) group. Apocynin treatment, with or without HBO preconditioning, improved creatinine and phosphate clearances, in postischemic AKI. This improvement in renal function was accompanied with decreased 4-HNE, while HO-1 kidney expression restored close to the control group level. NGAL renal expression was also decreased after apocynin treatment, and HBO preconditioning, with or without APO treatment. Considering our results, we can say that 4-HNE tissue expression can be used as a marker of oxidative stress in postischemic AKI. On the other hand, apocynin treatment and HBO preconditioning reduced oxidative damage, and this protective effect might be expected even in experimental hypertensive condition.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Antioxidants",
title = "Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats",
number = "8",
pages = "1163",
volume = "10",
doi = "10.3390/antiox10081163"
}

Kovačević, S., Ivanov, M., Životić, M., Brkić, P., Miloradović, Z., Jeremić, R., Mihailović-Stanojević, N., Vajić, U. J., Karanović, D., Jovović, Đ.,& Nešović-Ostojić, J.. (2021). Immunohistochemical Analysis of 4-HNE, NGAL, and HO-1 Tissue Expression after Apocynin Treatment and HBO Preconditioning in Postischemic Acute Kidney Injury Induced in Spontaneously Hypertensive Rats. in Antioxidants
Multidisciplinary Digital Publishing Institute (MDPI)., 10(8), 1163.
https://doi.org/10.3390/antiox10081163

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