TY  - JOUR
AU  - Pires, Ana Salomé
AU  - Bollini, Sveva
AU  - Botelho, Maria Filomena
AU  - Lang-Olip, Ingrid
AU  - Ponsaerts, Peter
AU  - Balbi, Carolina
AU  - Lange-Consiglio, Anna
AU  - Fénelon, Mathilde
AU  - Mojsilović, Slavko
AU  - Berishvili, Ekaterine
AU  - Cremonesi, Fausto
AU  - Gazouli, Maria
AU  - Bugarski, Diana
AU  - Gellhaus, Alexandra
AU  - Kerdjoudj, Halima
AU  - Schoeberlein, Andreina
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1297
AB  - The last 18 years have brought an increasing interest in the therapeutic use of perinatal derivatives (PnD). Preclinical studies used to assess the potential of PnD therapy include a broad range of study designs. The COST SPRINT Action (CA17116) aims to provide systematic and comprehensive reviews of preclinical studies for the understanding of the therapeutic potential and mechanisms of PnD in diseases and injuries that benefit from PnD therapy. Here we describe the publication search and data mining, extraction, and synthesis strategies employed to collect and prepare the published data selected for meta-analyses and reviews of the efficacy of PnD therapies for different diseases and injuries. A coordinated effort was made to prepare the data suitable to make statements for the treatment efficacy of the different types of PnD, routes, time points, and frequencies of administration, and the dosage based on clinically relevant effects resulting in clear increase, recovery or amelioration of the specific tissue or organ function. According to recently proposed guidelines, the harmonization of the nomenclature of PnD types will allow for the assessment of the most efficient treatments in various disease models. Experts within the COST SPRINT Action (CA17116), together with external collaborators, are doing the meta-analyses and reviews using the data prepared with the strategies presented here in the relevant disease or research fields. Our final aim is to provide standards to assess the safety and clinical benefit of PnD and to minimize redundancy in the use of animal models following the 3R principles for animal experimentation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Methods and Protocols
T1  - Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives
IS  - 3
SP  - 45
VL  - 6
DO  - 10.3390/mps6030045
ER  - 

@article{
author = "Pires, Ana Salomé and Bollini, Sveva and Botelho, Maria Filomena and Lang-Olip, Ingrid and Ponsaerts, Peter and Balbi, Carolina and Lange-Consiglio, Anna and Fénelon, Mathilde and Mojsilović, Slavko and Berishvili, Ekaterine and Cremonesi, Fausto and Gazouli, Maria and Bugarski, Diana and Gellhaus, Alexandra and Kerdjoudj, Halima and Schoeberlein, Andreina",
year = "2023",
abstract = "The last 18 years have brought an increasing interest in the therapeutic use of perinatal derivatives (PnD). Preclinical studies used to assess the potential of PnD therapy include a broad range of study designs. The COST SPRINT Action (CA17116) aims to provide systematic and comprehensive reviews of preclinical studies for the understanding of the therapeutic potential and mechanisms of PnD in diseases and injuries that benefit from PnD therapy. Here we describe the publication search and data mining, extraction, and synthesis strategies employed to collect and prepare the published data selected for meta-analyses and reviews of the efficacy of PnD therapies for different diseases and injuries. A coordinated effort was made to prepare the data suitable to make statements for the treatment efficacy of the different types of PnD, routes, time points, and frequencies of administration, and the dosage based on clinically relevant effects resulting in clear increase, recovery or amelioration of the specific tissue or organ function. According to recently proposed guidelines, the harmonization of the nomenclature of PnD types will allow for the assessment of the most efficient treatments in various disease models. Experts within the COST SPRINT Action (CA17116), together with external collaborators, are doing the meta-analyses and reviews using the data prepared with the strategies presented here in the relevant disease or research fields. Our final aim is to provide standards to assess the safety and clinical benefit of PnD and to minimize redundancy in the use of animal models following the 3R principles for animal experimentation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Methods and Protocols",
title = "Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives",
number = "3",
pages = "45",
volume = "6",
doi = "10.3390/mps6030045"
}

Pires, A. S., Bollini, S., Botelho, M. F., Lang-Olip, I., Ponsaerts, P., Balbi, C., Lange-Consiglio, A., Fénelon, M., Mojsilović, S., Berishvili, E., Cremonesi, F., Gazouli, M., Bugarski, D., Gellhaus, A., Kerdjoudj, H.,& Schoeberlein, A.. (2023). Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives. in Methods and Protocols
Multidisciplinary Digital Publishing Institute (MDPI)., 6(3), 45.
https://doi.org/10.3390/mps6030045

Pires AS, Bollini S, Botelho MF, Lang-Olip I, Ponsaerts P, Balbi C, Lange-Consiglio A, Fénelon M, Mojsilović S, Berishvili E, Cremonesi F, Gazouli M, Bugarski D, Gellhaus A, Kerdjoudj H, Schoeberlein A. Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives. in Methods and Protocols. 2023;6(3):45.
doi:10.3390/mps6030045 .

Pires, Ana Salomé, Bollini, Sveva, Botelho, Maria Filomena, Lang-Olip, Ingrid, Ponsaerts, Peter, Balbi, Carolina, Lange-Consiglio, Anna, Fénelon, Mathilde, Mojsilović, Slavko, Berishvili, Ekaterine, Cremonesi, Fausto, Gazouli, Maria, Bugarski, Diana, Gellhaus, Alexandra, Kerdjoudj, Halima, Schoeberlein, Andreina, "Guidelines to Analyze Preclinical Studies Using Perinatal Derivatives" in Methods and Protocols, 6, no. 3 (2023):45,
https://doi.org/10.3390/mps6030045 . .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Vujačić, Marko
AU  - Arsić, Aleksandra
AU  - Bogosavljević, Nikola
AU  - Kovačić, Marijana
AU  - Drvenica, Ivana
AU  - Mojsilović, Slavko
AU  - Baščarević, Zoran
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1408
AB  - Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients
IS  - 3
SP  - P140
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1408
ER  - 

@conference{
author = "Trivanović, Drenka and Vujačić, Marko and Arsić, Aleksandra and Bogosavljević, Nikola and Kovačić, Marijana and Drvenica, Ivana and Mojsilović, Slavko and Baščarević, Zoran and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Bone marrow (BM) adipose tissue (BMAT) has been described as lipotoxic factor with negative impacts on skeletal system regeneration and repair. As BMAT undergoes metabolic and cellular 
adaptations with age and disease, we assumed that investigation of BMAT-associated lipid profile and cellularity at different skeletal locations in osteoarthritis (OA) patients might contribute to understanding of lipid involvement in OA development and progression.Acetabular and femoral BM, and femoral subcutaneous adipose tissue (fSAT) were obtained from 
matched patients (n=11, 5 women, 6 men; age: 65±11 years; BMI: 27.89±4.42 kg/m2) undergoing hip arthroplasty surgery (Ethical approval I-97/11). BM, BMAT and fSAT were explored at the levels of total lipids, fatty acids, and cells, by using thin layer and gas chromatography and ex vivo cellular 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License assays. Statistical significance was estimated by non-parametric tests and Spearman’s rank correlation (r) was calculated.BMAT content was significantly higher in femoral (0.262±0.088 mL/g) than in acetabular BM (0.063±0.051 mL/g) (n=11, p=0.016). Negative associations with BMI of patients were found for femoral BM (r=-0.783, p=0.017, n=11) and BMAT (n=9, r=-1.000, p=0.017) tissue cellularity. 
Additionally, femoral BMAT cellularity declined with age (r=-0.675, n=10, p=0.037). Total lipid analyses revealed significantly lower triglyceride content in femoral than in acetabular BMAT and fSAT. Frequency of saturated palmitic, myristic and stearic acids were higher in femoral than in acetabular BMAT and fSAT, where palmitoleic, linoleic, oleic acids were more dominant. BMAT associated compartments from both locations host lower frequency of non-hematopoietic CD45- neutral lipid-loaded cells when compared to BM. This associated with higher incidence of clonogenic mesenchymal stromal (stem) cells in acetabular (0.032± 0.04%) and femoral (0.021± 0.028%) BMATs and fSAT (0.031 ± 0.016%) than in their BM counterparts.
Collectively, our results indicate that the lipid profiles of hip BMAT impose significantly different BM microenvironments and distribution of cells with regenerative potential in OA patients.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients",
number = "3",
pages = "P140",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1408"
}

Trivanović, D., Vujačić, M., Arsić, A., Bogosavljević, N., Kovačić, M., Drvenica, I., Mojsilović, S., Baščarević, Z., Bugarski, D.,& Jauković, A.. (2023). Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408

Trivanović D, Vujačić M, Arsić A, Bogosavljević N, Kovačić M, Drvenica I, Mojsilović S, Baščarević Z, Bugarski D, Jauković A. Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P140.
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

Trivanović, Drenka, Vujačić, Marko, Arsić, Aleksandra, Bogosavljević, Nikola, Kovačić, Marijana, Drvenica, Ivana, Mojsilović, Slavko, Baščarević, Zoran, Bugarski, Diana, Jauković, Aleksandra, "Lipid and cellular profiles of acetabular and femoral bone marrow adipose tissues are distinct in hip osteoarthritis patients" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P140,
https://hdl.handle.net/21.15107/rcub_rimi_1408 .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Labella, Rossella
AU  - Tratwal, Josefine
AU  - Bugarski, Diana
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1268
PB  - Frontiers Media S.A.
T2  - Frontiers in Cell and Developmental Biology
T1  - Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease
SP  - 1095235
VL  - 10
DO  - 10.3389/fcell.2022.1095235
ER  - 

@article{
author = "Trivanović, Drenka and Labella, Rossella and Tratwal, Josefine and Bugarski, Diana",
year = "2023",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cell and Developmental Biology",
title = "Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease",
pages = "1095235",
volume = "10",
doi = "10.3389/fcell.2022.1095235"
}

Trivanović, D., Labella, R., Tratwal, J.,& Bugarski, D.. (2023). Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease. in Frontiers in Cell and Developmental Biology
Frontiers Media S.A.., 10, 1095235.
https://doi.org/10.3389/fcell.2022.1095235

Trivanović D, Labella R, Tratwal J, Bugarski D. Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease. in Frontiers in Cell and Developmental Biology. 2023;10:1095235.
doi:10.3389/fcell.2022.1095235 .

Trivanović, Drenka, Labella, Rossella, Tratwal, Josefine, Bugarski, Diana, "Editorial: Regional and molecular fingerprint of adipogenesis in aging and disease" in Frontiers in Cell and Developmental Biology, 10 (2023):1095235,
https://doi.org/10.3389/fcell.2022.1095235 . .

TY  - CONF
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Živanović, Milena
AU  - Vujačić, Marko
AU  - Bogosavljević, Nikola
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2023
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1409
AB  - Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.
PB  - American Society for Bone and Mineral Research
C3  - JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
T1  - Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation
IS  - 3
SP  - P141
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_rimi_1409
ER  - 

@conference{
author = "Trivanović, Drenka and Okić Đorđević, Ivana and Živanović, Milena and Vujačić, Marko and Bogosavljević, Nikola and Bugarski, Diana and Jauković, Aleksandra",
year = "2023",
abstract = "Aging and disease-induced adipogenesis in skeletal system has been described as detrimental process for bone tissue metabolism. Dynamic of adipogenic program is controlled by microenvironmental factors and activity of bone marrow (BM) mesenchymal stromal (stem) cells (MSC)s. As different skeletal locations are not affected by extrinsic factors in same manner, we assumed that marrow adipogenic program can be distinct in acetabular (aMSCs) and femoral MSCs (fMSCs). Here, we compared expanded aMSCs and fMSCs from matched patients undergoing hip arthroplasty (n=6, Ethical approval I-97/11). Cellular and molecular assays were performed to investigate differences in MSC features. Statistical significance was estimated by ANOVA. Results showed that adipogenic stimuli triggered stronger adipogenesis in fMSCs when compared to acetabular counterparts (p=0.036). Tissue non-specific alkaline phosphatase (TNAP) activity and protein expression was higher in fMSCs than in aMSCs, along with significantly higher TNAP levels detected in mitochondrial-enriched fraction proteins in fMSCs. Stronger expression of mitochondrial electron transport chain (ETC) proteins, supercomplexes I and V was found in fMSCs than in aMSCs. This coincided with increased β-galactosidase and total intracellular reactive oxygen species (ROS) production in fMSCs. Lipid droplet accumulation was followed by upregulated tissue beta-galactosidase and TNAP activities, expression of glyceraldehyde 3-phosphate dehydrogenase 24734039, 2023, S3, Downloaded from https://asbmr.onlinelibrary.wiley.com/doi/10.1002/jbm4.10738 by Readcube (Labtiva Inc.), Wiley Online Library on [23/01/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License (GAPDH), in parallel with stimulated ROS and mitochondrial superoxide production in both MSCs. Presence of fatty acid oxidation (FAO) inhibitor etomoxir increased gene expression of fatty acid binding protein (Fabp)4, while decreased protein and gene expression of GAPDH in both populations. Although etomoxir supported adipogenic differentiation and β-galactosidase activity in aMSCs only, TNAP activity and ROS content stayed unaltered.These results indicate that mitochondrial pathways required for energy production, ETC and FAO are bone-specific, and differently affect marrow adipogenesis in acetabular and femoral regions. Further elucidation of marrow adipogenesis can contribute to development of pharmacologic strategies to support skeletal and metabolic health.",
publisher = "American Society for Bone and Mineral Research",
journal = "JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool",
title = "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation",
number = "3",
pages = "P141",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_rimi_1409"
}

Trivanović, D., Okić Đorđević, I., Živanović, M., Vujačić, M., Bogosavljević, N., Bugarski, D.,& Jauković, A.. (2023). Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool
American Society for Bone and Mineral Research., 7(3), P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409

Trivanović D, Okić Đorđević I, Živanović M, Vujačić M, Bogosavljević N, Bugarski D, Jauković A. Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation. in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool. 2023;7(3):P141.
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

Trivanović, Drenka, Okić Đorđević, Ivana, Živanović, Milena, Vujačić, Marko, Bogosavljević, Nikola, Bugarski, Diana, Jauković, Aleksandra, "Region-specific differences of marrow adipogenesis in mesenchymal stromal (stem) cells of human acetabulum and femur: involvement of fatty acid oxidation" in JBMR PlusVolume 7: Abstracts of the 50th ECTS Congress featuring BRS Annual Meeting, 15-18 April 2023, Liverpool, 7, no. 3 (2023):P141,
https://hdl.handle.net/21.15107/rcub_rimi_1409 .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Miletić, Maja
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1184
AB  - Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.
PB  - Srpsko biološko društvo
T2  - Archives of Biological Sciences
T1  - Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway
EP  - 24
IS  - 1
SP  - 15
VL  - 74
DO  - 10.2298/ABS210929048O
ER  - 

@article{
author = "Okić Đorđević, Ivana and Kukolj, Tamara and Obradović, Hristina and Trivanović, Drenka and Petrović, Anđelija and Mojsilović, Slavko and Miletić, Maja and Bugarski, Diana and Jauković, Aleksandra",
year = "2022",
abstract = "Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.",
publisher = "Srpsko biološko društvo",
journal = "Archives of Biological Sciences",
title = "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway",
pages = "24-15",
number = "1",
volume = "74",
doi = "10.2298/ABS210929048O"
}

Okić Đorđević, I., Kukolj, T., Obradović, H., Trivanović, D., Petrović, A., Mojsilović, S., Miletić, M., Bugarski, D.,& Jauković, A.. (2022). Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences
Srpsko biološko društvo., 74(1), 15-24.
https://doi.org/10.2298/ABS210929048O

Okić Đorđević I, Kukolj T, Obradović H, Trivanović D, Petrović A, Mojsilović S, Miletić M, Bugarski D, Jauković A. Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway. in Archives of Biological Sciences. 2022;74(1):15-24.
doi:10.2298/ABS210929048O .

Okić Đorđević, Ivana, Kukolj, Tamara, Obradović, Hristina, Trivanović, Drenka, Petrović, Anđelija, Mojsilović, Slavko, Miletić, Maja, Bugarski, Diana, Jauković, Aleksandra, "Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway" in Archives of Biological Sciences, 74, no. 1 (2022):15-24,
https://doi.org/10.2298/ABS210929048O . .

TY  - JOUR
AU  - Borojević, Ana
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Mojsilović, Slavko
AU  - Obradović, Hristina
AU  - Trivanović, Drenka
AU  - Živanović, Milena
AU  - Zečević, Željko
AU  - Simić, Marija
AU  - Gobeljić, Borko
AU  - Vujić, Dragana
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1207
AB  - The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Biomolecules
T1  - Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
IS  - 2
SP  - 323
VL  - 12
DO  - 10.3390/biom12020323
ER  - 

@article{
author = "Borojević, Ana and Jauković, Aleksandra and Kukolj, Tamara and Mojsilović, Slavko and Obradović, Hristina and Trivanović, Drenka and Živanović, Milena and Zečević, Željko and Simić, Marija and Gobeljić, Borko and Vujić, Dragana and Bugarski, Diana",
year = "2022",
abstract = "The biology of vitamin D3 is well defined, as are the effects of its active metabolites on various cells, including mesenchymal stromal/stem cells (MSCs). However, the biological potential of its precursor, cholecalciferol (VD3), has not been sufficiently investigated, although its significance in regenerative medicine—mainly in combination with various biomaterial matrices—has been recognized. Given that VD3 preconditioning might also contribute to the improvement of cellular regenerative potential, the aim of this study was to investigate its effects on bone marrow (BM) MSC functions and the signaling pathways involved. For that purpose, the influence of VD3 on BM-MSCs obtained from young human donors was determined via MTT test, flow cytometric analysis, immunocytochemistry, and qRT-PCR. Our results revealed that VD3, following a 5-day treatment, stimulated proliferation, expression of pluripotency markers (NANOG, SOX2, and Oct4), and osteogenic differentiation potential in BM-MSCs, while it reduced their senescence. Moreover, increased sirtuin 1 (SIRT1) expression was detected upon treatment with VD3, which mediated VD3-promoted osteogenesis and, partially, the stemness features through NANOG and SOX2 upregulation. In contrast, the effects of VD3 on proliferation, Oct4 expression, and senescence were SIRT1-independent. Altogether, these data indicate that VD3 has strong potential to modulate BM-MSCs’ features, partially through SIRT1 signaling, although the precise mechanisms merit further investigation.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Biomolecules",
title = "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling",
number = "2",
pages = "323",
volume = "12",
doi = "10.3390/biom12020323"
}

Borojević, A., Jauković, A., Kukolj, T., Mojsilović, S., Obradović, H., Trivanović, D., Živanović, M., Zečević, Ž., Simić, M., Gobeljić, B., Vujić, D.,& Bugarski, D.. (2022). Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules
Multidisciplinary Digital Publishing Institute (MDPI)., 12(2), 323.
https://doi.org/10.3390/biom12020323

Borojević A, Jauković A, Kukolj T, Mojsilović S, Obradović H, Trivanović D, Živanović M, Zečević Ž, Simić M, Gobeljić B, Vujić D, Bugarski D. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. in Biomolecules. 2022;12(2):323.
doi:10.3390/biom12020323 .

Borojević, Ana, Jauković, Aleksandra, Kukolj, Tamara, Mojsilović, Slavko, Obradović, Hristina, Trivanović, Drenka, Živanović, Milena, Zečević, Željko, Simić, Marija, Gobeljić, Borko, Vujić, Dragana, Bugarski, Diana, "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling" in Biomolecules, 12, no. 2 (2022):323,
https://doi.org/10.3390/biom12020323 . .

TY  - JOUR
AU  - Vignjević-Petrinović, Sanja
AU  - Jauković, Aleksandra
AU  - Milošević, Maja
AU  - Bugarski, Diana
AU  - Budeč, Mirela
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1241
AB  - Cancer-related anemia (CRA) is a common multifactorial disorder that adversely affects the quality of life and overall prognosis in patients with cancer. Safety concerns associated with the most common CRA treatment options, including intravenous iron therapy and erythropoietic-stimulating agents, have often resulted in no or suboptimal anemia management for many cancer patients. Chronic anemia creates a vital need to restore normal erythropoietic output and therefore activates the mechanisms of stress erythropoiesis (SE). A growing body of evidence demonstrates that bone morphogenetic protein 4 (BMP4) signaling, along with glucocorticoids, erythropoietin, stem cell factor, growth differentiation factor 15 (GDF15) and hypoxia-inducible factors, plays a pivotal role in SE. Nevertheless, a chronic state of SE may lead to ineffective erythropoiesis, characterized by the expansion of erythroid progenitor pool, that largely fails to differentiate and give rise to mature red blood cells, further aggravating CRA. In this review, we summarize the current state of knowledge on the emerging roles for stress erythroid progenitors and activated SE pathways in tumor progression, highlighting the urgent need to suppress ineffective erythropoiesis in cancer patients and develop an optimal treatment strategy as well as a personalized approach to CRA management.
PB  - Frontiers Media S.A
T2  - Frontiers in Physiology
T1  - Targeting Stress Erythropoiesis Pathways in Cancer
SP  - 844042
VL  - 13
DO  - 10.3389/fphys.2022.844042
ER  - 

@article{
author = "Vignjević-Petrinović, Sanja and Jauković, Aleksandra and Milošević, Maja and Bugarski, Diana and Budeč, Mirela",
year = "2022",
abstract = "Cancer-related anemia (CRA) is a common multifactorial disorder that adversely affects the quality of life and overall prognosis in patients with cancer. Safety concerns associated with the most common CRA treatment options, including intravenous iron therapy and erythropoietic-stimulating agents, have often resulted in no or suboptimal anemia management for many cancer patients. Chronic anemia creates a vital need to restore normal erythropoietic output and therefore activates the mechanisms of stress erythropoiesis (SE). A growing body of evidence demonstrates that bone morphogenetic protein 4 (BMP4) signaling, along with glucocorticoids, erythropoietin, stem cell factor, growth differentiation factor 15 (GDF15) and hypoxia-inducible factors, plays a pivotal role in SE. Nevertheless, a chronic state of SE may lead to ineffective erythropoiesis, characterized by the expansion of erythroid progenitor pool, that largely fails to differentiate and give rise to mature red blood cells, further aggravating CRA. In this review, we summarize the current state of knowledge on the emerging roles for stress erythroid progenitors and activated SE pathways in tumor progression, highlighting the urgent need to suppress ineffective erythropoiesis in cancer patients and develop an optimal treatment strategy as well as a personalized approach to CRA management.",
publisher = "Frontiers Media S.A",
journal = "Frontiers in Physiology",
title = "Targeting Stress Erythropoiesis Pathways in Cancer",
pages = "844042",
volume = "13",
doi = "10.3389/fphys.2022.844042"
}

Vignjević-Petrinović, S., Jauković, A., Milošević, M., Bugarski, D.,& Budeč, M.. (2022). Targeting Stress Erythropoiesis Pathways in Cancer. in Frontiers in Physiology
Frontiers Media S.A., 13, 844042.
https://doi.org/10.3389/fphys.2022.844042

Vignjević-Petrinović S, Jauković A, Milošević M, Bugarski D, Budeč M. Targeting Stress Erythropoiesis Pathways in Cancer. in Frontiers in Physiology. 2022;13:844042.
doi:10.3389/fphys.2022.844042 .

Vignjević-Petrinović, Sanja, Jauković, Aleksandra, Milošević, Maja, Bugarski, Diana, Budeč, Mirela, "Targeting Stress Erythropoiesis Pathways in Cancer" in Frontiers in Physiology, 13 (2022):844042,
https://doi.org/10.3389/fphys.2022.844042 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Lazarević, Jasmina
AU  - Borojević, Ana
AU  - Ralević, Uroš
AU  - Vujić, Dragana
AU  - Jauković, Aleksandra
AU  - Lazarević, Nenad
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1235
AB  - The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity
IS  - 9
SP  - 4915
VL  - 23
DO  - 10.3390/ijms23094915
ER  - 

@article{
author = "Kukolj, Tamara and Lazarević, Jasmina and Borojević, Ana and Ralević, Uroš and Vujić, Dragana and Jauković, Aleksandra and Lazarević, Nenad and Bugarski, Diana",
year = "2022",
abstract = "The heterogeneity of stem cells represents the main challenge in regenerative medicine development. This issue is particularly pronounced when it comes to the use of primary mesenchymal stem/stromal cells (MSCs) due to a lack of identification markers. Considering the need for additional approaches in MSCs characterization, we applied Raman spectroscopy to investigate inter-individual differences between bone marrow MSCs (BM-MSCs). Based on standard biological tests, BM-MSCs of analyzed donors fulfill all conditions for their characterization, while no donor-related specifics were observed in terms of BM-MSCs morphology, phenotype, multilineage differentiation potential, colony-forming capacity, expression of pluripotency-associated markers or proliferative capacity. However, examination of BM-MSCs at a single-cell level by Raman spectroscopy revealed that despite similar biochemical background, fine differences in the Raman spectra of BM-MSCs of each donor can be detected. After extensive principal component analysis (PCA) of Raman spectra, our study revealed the possibility of this method to diversify BM-MSCs populations, whereby the grouping of cell populations was most prominent when cell populations were analyzed in pairs. These results indicate that Raman spectroscopy, as a label-free assay, could have a huge potential in understanding stem cell heterogeneity and sorting cell populations with a similar biochemical background that can be significant for the development of personalized therapy approaches.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity",
number = "9",
pages = "4915",
volume = "23",
doi = "10.3390/ijms23094915"
}

Kukolj, T., Lazarević, J., Borojević, A., Ralević, U., Vujić, D., Jauković, A., Lazarević, N.,& Bugarski, D.. (2022). A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences
MDPI., 23(9), 4915.
https://doi.org/10.3390/ijms23094915

Kukolj T, Lazarević J, Borojević A, Ralević U, Vujić D, Jauković A, Lazarević N, Bugarski D. A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity. in International Journal of Molecular Sciences. 2022;23(9):4915.
doi:10.3390/ijms23094915 .

Kukolj, Tamara, Lazarević, Jasmina, Borojević, Ana, Ralević, Uroš, Vujić, Dragana, Jauković, Aleksandra, Lazarević, Nenad, Bugarski, Diana, "A Single-Cell Raman Spectroscopy Analysis of Bone Marrow Mesenchymal Stem/Stromal Cells to Identify Inter-Individual Diversity" in International Journal of Molecular Sciences, 23, no. 9 (2022):4915,
https://doi.org/10.3390/ijms23094915 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Ilić, Vesna
AU  - Bugarski, Branko
AU  - Bugarski, Diana
PY  - 2022
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1187
AB  - Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.
PB  - MDPI
T2  - Processes
T1  - Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin
IS  - 1
SP  - 32
VL  - 10
DO  - 10.3390/pr10010032
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Ilić, Vesna and Bugarski, Branko and Bugarski, Diana",
year = "2022",
abstract = "Exploring the potential usage of the acellular preparation of porcine hemoglobin (PHb) isolated from slaughterhouse blood as a cell culture media component, we have tested its effects on the functional characteristics of stromal cells of mesodermal origin. Human peripheral blood mesenchymal stromal cells (PB-MSCs) were used in this study as a primary cell model system, along with three mouse cell lines (ATDC5, MC3T3-E1, and 3T3-L1), which represent more uniform model systems. We investigated the effect of PHb at concentrations of 0.1, 1, and 10 μM on these cells’ proliferation, cycle, and clonogenic and migratory potential, and found that PHb’s effect depended on both the cell type and its concentration. At the lowest concentration used (0.1 μM), PHb showed the least evident impact on the cell growth and migration; hence, we analyzed its effect on mesenchymal cell multilineage differentiation capacity at this concentration. Even under conditions that induce a specific type of MSC differentiation (cultivation in particular differentiation media), PHb modulated chondrogenic, osteogenic, and adipogenic differentiation, making it a potential candidate for a supplement of MSC culture. Through a model of porcine hemoglobin, these findings also contribute to improving the knowledge of extracellular hemoglobin’s influence on MSCs in vivo.",
publisher = "MDPI",
journal = "Processes",
title = "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin",
number = "1",
pages = "32",
volume = "10",
doi = "10.3390/pr10010032"
}

Stančić, A., Drvenica, I., Ilić, V., Bugarski, B.,& Bugarski, D.. (2022). Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes
MDPI., 10(1), 32.
https://doi.org/10.3390/pr10010032

Stančić A, Drvenica I, Ilić V, Bugarski B, Bugarski D. Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin. in Processes. 2022;10(1):32.
doi:10.3390/pr10010032 .

Stančić, Ana, Drvenica, Ivana, Ilić, Vesna, Bugarski, Branko, Bugarski, Diana, "Modulation of Functional Characteristics of Mesenchymal Stromal Cells by Acellular Preparation of Porcine Hemoglobin" in Processes, 10, no. 1 (2022):32,
https://doi.org/10.3390/pr10010032 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Drvenica, Ivana
AU  - Stančić, Ana
AU  - Mojsilović, Slavko
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Saso, Luciano
AU  - Nicoletti, Marcello
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1086
AB  - Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration  gt = 500 mu g/mL. The antioxidant activity of hennosides (concentration  gt = 100 mu g/mL) was determined by FRAP and ABTS assays. At concentration of 500 mu g/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 +/- 0.08, 0.62 +/- 0.28 and 0.35 +/- 0.03 mM FeSO4 x 7H(2)O, and 0.15 +/- 0.01, 0.30 +/- 0.01 and 0.09 +/- 0.01 mM Trolox. Hennosides at 100 mu g/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.
PB  - MDPI
T2  - Plants-Basel
T1  - Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead
IS  - 2
SP  - 237
VL  - 10
DO  - 10.3390/plants10020237
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Drvenica, Ivana and Stančić, Ana and Mojsilović, Slavko and Kukolj, Tamara and Bugarski, Diana and Saso, Luciano and Nicoletti, Marcello",
year = "2021",
abstract = "Henna is the current name of the dye prepared from the dry leaf powder of Lawsonia inermis (Lythraceae). Several studies have focused on the chemistry and pharmacology of the henna dyeing active compound, lawsone, obtained from the main constituents of leaves, hennosides, during the processing of plant material. However, knowledge regarding the biological activity of hennosides is largely lacking. In this paper, the redox activity of three hennoside isomers is reported. The pro-oxidative activity was confirmed by their ability to induce mild lysis of erythrocytes and to increase the level of methemoglobin at the concentration  gt = 500 mu g/mL. The antioxidant activity of hennosides (concentration  gt = 100 mu g/mL) was determined by FRAP and ABTS assays. At concentration of 500 mu g/mL, antioxidant activity of hennoside isomers was equivalent to 0.46 +/- 0.08, 0.62 +/- 0.28 and 0.35 +/- 0.03 mM FeSO4 x 7H(2)O, and 0.15 +/- 0.01, 0.30 +/- 0.01 and 0.09 +/- 0.01 mM Trolox. Hennosides at 100 mu g/mL concentration did not influence viability of human breast cancer cell lines MDA231 and MCF-7 and primary human peripheral blood and periodontal ligament-mesenchymal stem cells, but produced a modest increase in concentration of antioxidants in the cell culture supernatants. The evidenced antioxidant and pro-oxidant activities indicate their potential to act as redox balance regulator, which opens up the possibility of using hennosides in commercial phytomedicines.",
publisher = "MDPI",
journal = "Plants-Basel",
title = "Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead",
number = "2",
pages = "237",
volume = "10",
doi = "10.3390/plants10020237"
}

Maslovarić, I., Ilić, V., Drvenica, I., Stančić, A., Mojsilović, S., Kukolj, T., Bugarski, D., Saso, L.,& Nicoletti, M.. (2021). Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. in Plants-Basel
MDPI., 10(2), 237.
https://doi.org/10.3390/plants10020237

Maslovarić I, Ilić V, Drvenica I, Stančić A, Mojsilović S, Kukolj T, Bugarski D, Saso L, Nicoletti M. Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead. in Plants-Basel. 2021;10(2):237.
doi:10.3390/plants10020237 .

Maslovarić, Irina, Ilić, Vesna, Drvenica, Ivana, Stančić, Ana, Mojsilović, Slavko, Kukolj, Tamara, Bugarski, Diana, Saso, Luciano, Nicoletti, Marcello, "Insight into the Biological Activity of Hennosides-Glucosides Isolated from Lawsonia inermis (henna): Could They Be Regarded as Active Constituents Instead" in Plants-Basel, 10, no. 2 (2021):237,
https://doi.org/10.3390/plants10020237 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Miletić, Maja
AU  - Petrović, Vanja
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1149
AB  - Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.
PB  - Wiley-Blackwell
T2  - Journal of Cellular Physiology
T1  - Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF
EP  - 7341
IS  - 11
SP  - 7322
VL  - 236
DO  - 10.1002/jcp.30399
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Obradović, Hristina and Miletić, Maja and Petrović, Vanja and Mojsilović, Slavko and Bugarski, Diana",
year = "2021",
abstract = "Mesenchymal stem cells (MSCs) have been identified within dental pulp tissues of exfoliated deciduous (SHEDs) and permanent (DPSCs) teeth. Although differences in their proliferative and differentiation properties were revealed, variability in SHEDs and DPSCs responsiveness to growth factors and cytokines have not been studied before. Here, we investigated the influence of interleukin-17 (IL-17) and basic fibroblast growth factor (bFGF) on stemness features of SHEDs and DPSCs by analyzing their proliferation, clonogenicity, cell cycle progression, pluripotency markers expression and differentiation after 7-day treatment. Results indicated that IL-17 and bFGF differently affected SHEDs and DPSCs proliferation and clonogenicity, since bFGF increased proliferative and clonogenic potential of both cell types, while IL-17 similarly affected SHEDs, exerting no effects on adult counterparts DPSCs. In addition, both factors stimulated NANOG, OCT4, and SOX2 pluripotency markers expression in SHEDs and DPSCs showing diverse intracellular expression patterns dependent on MSCs type. As for the differentiation capacity, both factors displayed comparable effects on SHEDs and DPSCs, including stimulatory effect of IL-17 on early osteogenesis in contrast to the strong inhibitory effect showed for bFGF, while having no impact on SHEDs and DPSCs chondrogenesis. Moreover, bFGF combined with IL-17 reduced CD90 and stimulated CD73 expression on both types of MSCs, whereas each factor induced IL-6 expression indicating its' role in IL-17/bFGF-modulated properties of SHEDs and DPSCs. All these data demonstrated that dental pulp MSCs from primary and permanent teeth exert intrinsic features, providing novel evidence on how IL-17 and bFGF affect stem cell properties important for regeneration of dental pulp at different ages.",
publisher = "Wiley-Blackwell",
journal = "Journal of Cellular Physiology",
title = "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF",
pages = "7341-7322",
number = "11",
volume = "236",
doi = "10.1002/jcp.30399"
}

Jauković, A., Kukolj, T., Trivanović, D., Okić Đorđević, I., Obradović, H., Miletić, M., Petrović, V., Mojsilović, S.,& Bugarski, D.. (2021). Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology
Wiley-Blackwell., 236(11), 7322-7341.
https://doi.org/10.1002/jcp.30399

Jauković A, Kukolj T, Trivanović D, Okić Đorđević I, Obradović H, Miletić M, Petrović V, Mojsilović S, Bugarski D. Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF. in Journal of Cellular Physiology. 2021;236(11):7322-7341.
doi:10.1002/jcp.30399 .

Jauković, Aleksandra, Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Obradović, Hristina, Miletić, Maja, Petrović, Vanja, Mojsilović, Slavko, Bugarski, Diana, "Modulating stemness of mesenchymal stem cells from exfoliated deciduous and permanent teeth by IL-17 and bFGF" in Journal of Cellular Physiology, 236, no. 11 (2021):7322-7341,
https://doi.org/10.1002/jcp.30399 . .

TY  - JOUR
AU  - Mojsilović, Slavko
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Petrović, Anđelija
AU  - Bugarski, Diana
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1175
AB  - As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.
PB  - Multidisciplinary Digital Publishing Institute (MDPI)
T2  - Journal of Personalized Medicine
T1  - Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy
IS  - 11
SP  - 1133
VL  - 11
DO  - 10.3390/jpm11111133
ER  - 

@article{
author = "Mojsilović, Slavko and Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Petrović, Anđelija and Bugarski, Diana",
year = "2021",
abstract = "As an organism ages, many physiological processes change, including the immune system. This process, called immunosenescence, characterized by abnormal activation and imbalance of innate and adaptive immunity, leads to a state of chronic low-grade systemic inflammation, termed inflammaging. Aging and inflammaging are considered to be the root of many diseases of the elderly, as infections, autoimmune and chronic inflammatory diseases, degenerative diseases, and cancer. The role of mesenchymal stromal/stem cells (MSCs) in the inflammaging process and the age-related diseases is not completely established, although numerous features of aging MSCs, including altered immunomodulatory properties, impeded MSC niche supporting functions, and senescent MSC secretory repertoire are consistent with inflammaging development. Although senescence has its physiological function and can represent a mechanism of tumor prevention, in most cases it eventually transforms into a deleterious (para-)inflammatory process that promotes tumor growth. In this review we are going through current literature, trying to explore the role of senescent MSCs in making and/or sustaining a microenvironment permissive to tumor development and to analyze the therapeutic options that could target this process.",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
journal = "Journal of Personalized Medicine",
title = "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy",
number = "11",
pages = "1133",
volume = "11",
doi = "10.3390/jpm11111133"
}

Mojsilović, S., Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Petrović, A.,& Bugarski, D.. (2021). Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine
Multidisciplinary Digital Publishing Institute (MDPI)., 11(11), 1133.
https://doi.org/10.3390/jpm11111133

Mojsilović S, Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Petrović A, Bugarski D. Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy. in Journal of Personalized Medicine. 2021;11(11):1133.
doi:10.3390/jpm11111133 .

Mojsilović, Slavko, Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Petrović, Anđelija, Bugarski, Diana, "Tumorigenic Aspects of MSC Senescence—Implication in Cancer Development and Therapy" in Journal of Personalized Medicine, 11, no. 11 (2021):1133,
https://doi.org/10.3390/jpm11111133 . .

TY  - JOUR
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Kukolj, Tamara
AU  - Petrović, Anđelija
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2021
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1183
AB  - Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.
PB  - Baishideng Publishing Group Inc
T2  - World Journal of Stem Cells
T1  - Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy
EP  - 1880
IS  - 12
SP  - 1863
VL  - 13
DO  - 10.4252/wjsc.v13.i12.1863
ER  - 

@article{
author = "Okić Đorđević, Ivana and Obradović, Hristina and Kukolj, Tamara and Petrović, Anđelija and Mojsilović, Slavko and Bugarski, Diana and Jauković, Aleksandra",
year = "2021",
abstract = "Current research data reveal microenvironment as a significant modifier of physical functions, pathologic changes, as well as the therapeutic effects of stem cells. When comparing regeneration potential of various stem cell types used for cytotherapy and tissue engineering, mesenchymal stem cells (MSCs) are currently the most attractive cell source for bone and tooth regeneration due to their differentiation and immunomodulatory potential and lack of ethical issues associated with their use. The microenvironment of donors and recipients selected in cytotherapy plays a crucial role in regenerative potential of transplanted MSCs, indicating interactions of cells with their microenvironment indispensable in MSC-mediated bone and dental regeneration. Since a variety of MSC populations have been procured from different parts of the tooth and tooth-supporting tissues, MSCs of dental origin and their achievements in capacity to reconstitute various dental tissues have gained attention of many research groups over the years. This review discusses recent advances in comparative analyses of dental MSC regeneration potential with regards to their tissue origin and specific microenvironmental conditions, giving additional insight into the current clinical application of these cells.",
publisher = "Baishideng Publishing Group Inc",
journal = "World Journal of Stem Cells",
title = "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy",
pages = "1880-1863",
number = "12",
volume = "13",
doi = "10.4252/wjsc.v13.i12.1863"
}

Okić Đorđević, I., Obradović, H., Kukolj, T., Petrović, A., Mojsilović, S., Bugarski, D.,& Jauković, A.. (2021). Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells
Baishideng Publishing Group Inc., 13(12), 1863-1880.
https://doi.org/10.4252/wjsc.v13.i12.1863

Okić Đorđević I, Obradović H, Kukolj T, Petrović A, Mojsilović S, Bugarski D, Jauković A. Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy. in World Journal of Stem Cells. 2021;13(12):1863-1880.
doi:10.4252/wjsc.v13.i12.1863 .

Okić Đorđević, Ivana, Obradović, Hristina, Kukolj, Tamara, Petrović, Anđelija, Mojsilović, Slavko, Bugarski, Diana, Jauković, Aleksandra, "Dental mesenchymal stromal/stem cells in different microenvironments — implications in regenerative therapy" in World Journal of Stem Cells, 13, no. 12 (2021):1863-1880,
https://doi.org/10.4252/wjsc.v13.i12.1863 . .

TY  - JOUR
AU  - Jauković, Aleksandra
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/993
AB  - Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Stem Cells
T1  - Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators
EP  - 937
IS  - 9
SP  - 922
VL  - 12
DO  - 10.4252/wjsc.v12.i9.922
ER  - 

@article{
author = "Jauković, Aleksandra and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Bugarski, Diana",
year = "2020",
abstract = "Mesenchymal stromal/stem cells (MSCs) are adult stem cells of stromal origin that possess self-renewal capacity and the ability to differentiate into multiple mesodermal cell lineages. They play a critical role in tissue homeostasis and wound healing, as well as in regulating the inflammatory microenvironment through interactions with immune cells. Hence, MSCs have garnered great attention as promising candidates for tissue regeneration and cell therapy. Because the inflammatory niche plays a key role in triggering the reparative and immunomodulatory functions of MSCs, priming of MSCs with bioactive molecules has been proposed as a way to foster the therapeutic potential of these cells. In this paper, we review how soluble mediators of the inflammatory niche (cytokines and alarmins) influence the regenerative and immunomodulatory capacity of MSCs, highlighting the major advantages and concerns regarding the therapeutic potential of these inflammatory primed MSCs. The data summarized in this review may provide a significant starting point for future research on priming MSCs and establishing standardized methods for the application of preconditioned MSCs in cell therapy.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Stem Cells",
title = "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators",
pages = "937-922",
number = "9",
volume = "12",
doi = "10.4252/wjsc.v12.i9.922"
}

Jauković, A., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S.,& Bugarski, D.. (2020). Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells
Baishideng Publishing Group Inc, Pleasanton., 12(9), 922-937.
https://doi.org/10.4252/wjsc.v12.i9.922

Jauković A, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Bugarski D. Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators. in World Journal of Stem Cells. 2020;12(9):922-937.
doi:10.4252/wjsc.v12.i9.922 .

Jauković, Aleksandra, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Bugarski, Diana, "Inflammatory niche: Mesenchymal stromal cell priming by soluble mediators" in World Journal of Stem Cells, 12, no. 9 (2020):922-937,
https://doi.org/10.4252/wjsc.v12.i9.922 . .

TY  - JOUR
AU  - Maslovarić, Irina
AU  - Ilić, Vesna
AU  - Stančić, Ana
AU  - Santibanez, Juan F.
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Krstić, Jelena
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1003
AB  - Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function.
AB  - Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts
T1  - Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta
EP  - 33
IS  - 1
SP  - 18
VL  - 74
DO  - 10.2298/VETGL190414019M
ER  - 

@article{
author = "Maslovarić, Irina and Ilić, Vesna and Stančić, Ana and Santibanez, Juan F. and Trivanović, Drenka and Drvenica, Ivana and Krstić, Jelena and Mojsilović, Slavko and Okić Đorđević, Ivana and Bugarski, Diana",
year = "2020",
abstract = "Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months' frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20°C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters  gt  dancers  gt  football players; mean migratory capacity was: weightlifters = dancers  gt  football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players  gt  dancers  gt  weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, longterm, frozen-stored PPP preserves its potential to modify myoblast morphology and function., Uvod. Krvna plazma obogaćena leukocitima, plazma sa niskim sadržajem leukocita i plazma sa niskim sadržajem trombocita (platelet poor plasma; PPP) su produkti krvi koji se koriste za stimulaciju regeneracije mišića. U ovom radu smo ispitivali da li zamrzavana PPP osoba koje se bave različitim tipovima fizičke aktivnosti, usmerava C2C12 myoblaste u pravcu povećane proliferacije, migracije i miogene diferencijacije, i da li utiče na morfologiju/izgled miotuba. Materijal i metode. PPP osoba muškog pola starih 15-19 godina je izolovana iz krvi dizača tegova, fudbalera i profesionalnih igrača folklora, 12 sati nakon treninga. Uzorci PPP su čuvani šest meseci na -20ºC. Uticaj PPP na proliferaciju C2C12 ćelija je analiziran MTT testom, na migraciju "scratch" testom, a uticaj na miogenu diferencijaciju je analiziran na osnovu sposobnosti PPP da indukuju formiranje miotuba. Želatinolitička aktivnost PPP je analizirana gel-zimografijom. Rezultati i zaključak. Uzorci PPP su indukovali proliferaciju i migraciju C2C12 ćelija, a kapacitet da stimulišu proliferaciju je bio: dizači tegova  gt  igrači  gt  fudbaleri. Kapacitet PPP da utiču na migraciju C2C12 ćelija je bio: dizači tegova = igrači  gt  fudbaleri. Svi uzorci PPP su indukovali formiranje miotuba, ali su zapažene značajne interindividualne varijacije. PPP dizača tegova su indukovali formiranje okruglih miotuba, dok su miotube formirane u prisustvu PPP igrača i fudbalera bile izdužene. Površina miotuba se, zavisno od tipa fizičke aktivnosti, menjala po sledećem rasporedu: fudbaleri  gt  igrači  gt  dizači tegova. Želatinolitička aktivnost PPP je nagativno korelirala sa proliferacijom C2C12 ćelija. Rezultati ove studije pokazuju da PPP osoba koje se bave određenim tipom fizičke aktivnosti mogu da na specifičan način modulišu morfologiju/funciju mioblasta. Ovaj rezultat je od značaja za objašnjnje fiziološkog odgovora i adaptacije na vežbanje. On pokazuje i da PPP nakon dugotrajnog zamrzavanja imaju očuvanu spospbnost modifikovanja morfologije i funkcije mioblasta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts, Platelet-poor plazma sportista kao potencijalni induktor miogene diferencijacije C2C12 mioblasta",
pages = "33-18",
number = "1",
volume = "74",
doi = "10.2298/VETGL190414019M"
}

Maslovarić, I., Ilić, V., Stančić, A., Santibanez, J. F., Trivanović, D., Drvenica, I., Krstić, J., Mojsilović, S., Okić Đorđević, I.,& Bugarski, D.. (2020). Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 74(1), 18-33.
https://doi.org/10.2298/VETGL190414019M

Maslovarić I, Ilić V, Stančić A, Santibanez JF, Trivanović D, Drvenica I, Krstić J, Mojsilović S, Okić Đorđević I, Bugarski D. Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts. in Veterinarski glasnik. 2020;74(1):18-33.
doi:10.2298/VETGL190414019M .

Maslovarić, Irina, Ilić, Vesna, Stančić, Ana, Santibanez, Juan F., Trivanović, Drenka, Drvenica, Ivana, Krstić, Jelena, Mojsilović, Slavko, Okić Đorđević, Ivana, Bugarski, Diana, "Platelet-poor plasma of athletes is a potent inducer of Myogenic differentiation of C2C12 myoblasts" in Veterinarski glasnik, 74, no. 1 (2020):18-33,
https://doi.org/10.2298/VETGL190414019M . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Vignjević-Petrinović, Sanja
AU  - Okić Đorđević, Ivana
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Jauković, Aleksandra
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/995
AB  - Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cell & Developmental Biology
T1  - Adipogenesis in Different Body Depots and Tumor Development
VL  - 8
DO  - 10.3389/fcell.2020.571648
ER  - 

@article{
author = "Trivanović, Drenka and Vignjević-Petrinović, Sanja and Okić Đorđević, Ivana and Kukolj, Tamara and Bugarski, Diana and Jauković, Aleksandra",
year = "2020",
abstract = "Adipose tissue (AT) forms depots at different anatomical locations throughout the body, being in subcutaneous and visceral regions, as well as the bone marrow. These ATs differ in the adipocyte functional profile, their insulin sensitivity, adipokines' production, lipolysis, and response to pathologic conditions. Despite the recent advances in lineage tracing, which have demonstrated that individual adipose depots are composed of adipocytes derived from distinct progenitor populations, the cellular and molecular dissection of the adipose clonogenic stem cell niche is still a great challenge. Additional complexity in AT regulation is associated with tumor-induced changes that affect adipocyte phenotype. As an integrative unit of cell differentiation, AT microenvironment regulates various phenotype outcomes of differentiating adipogenic lineages, which consequently may contribute to the neoplastic phenotype manifestations. Particularly interesting is the capacity of AT to impose and support the aberrant potency of stem cells that accompanies tumor development. In this review, we summarize the current findings on the communication between adipocytes and their progenitors with tumor cells, pointing out to the co-existence of healthy and neoplastic stem cell niches developed during tumor evolution. We also discuss tumor-induced adaptations in mature adipocytes and the involvement of alternative differentiation programs.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cell & Developmental Biology",
title = "Adipogenesis in Different Body Depots and Tumor Development",
volume = "8",
doi = "10.3389/fcell.2020.571648"
}

Trivanović, D., Vignjević-Petrinović, S., Okić Đorđević, I., Kukolj, T., Bugarski, D.,& Jauković, A.. (2020). Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fcell.2020.571648

Trivanović D, Vignjević-Petrinović S, Okić Đorđević I, Kukolj T, Bugarski D, Jauković A. Adipogenesis in Different Body Depots and Tumor Development. in Frontiers in Cell & Developmental Biology. 2020;8.
doi:10.3389/fcell.2020.571648 .

Trivanović, Drenka, Vignjević-Petrinović, Sanja, Okić Đorđević, Ivana, Kukolj, Tamara, Bugarski, Diana, Jauković, Aleksandra, "Adipogenesis in Different Body Depots and Tumor Development" in Frontiers in Cell & Developmental Biology, 8 (2020),
https://doi.org/10.3389/fcell.2020.571648 . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/983
AB  - Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation
EP  - 391
IS  - 3
SP  - 379
VL  - 72
DO  - 10.2298/ABS200625032S
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "Functional characteristics of satellite cells (SCs) that act as myogenesis initiators and have emerged as a promising target for cell therapy, are dependent on their microenvironment. The aim of this study was to investigate the effect of cell-free hemoglobin, as a part of the microenvironment of SCs, on their functional characteristics. The C2C12 cell line served as the experimental model of SCs; hemoglobin isolated from porcine (PHb) and bovine (BHb) slaughterhouse blood served as the experimental model for extracellular hemoglobin. The proliferation rate of C2C12 cells was assessed by the MTT test, migration capacity by the scratch assay, and myogenic differentiation capacity by histochemical staining and RT-PCR analysis of the expression of genes specific for myogenic lineage. The effect of hemoglobin on the proliferation and migration of C2C12 cells was dependent on its concentration and the animal species it was isolated from, but the effect of BHb was more prominent. Both PHb and BHb decreased the expression levels of myogenin and muscle specific creatine kinase at a 10 mu M concentration. While PHb had no effect on the morphometric parameters of C2C12 myotubes, BHb modified the area and length of C2C12 myotubes cultivated in DMEM/2% horse serum and DMEM/10% fetal calf serum. While PHb and BHb had no effect on heme oxygenase 1 (Hmox1) expression, they stimulated the expression of hypoxia-inducible factor 1-alpha (Hif1 alpha) at a concentration of 10 mu M. The mainly inhibitory effect of cell-free hemoglobin on myogenic differentiation suggests that it could be a relevant factor in the outcome of cell therapy of muscle injury.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation",
pages = "391-379",
number = "3",
volume = "72",
doi = "10.2298/ABS200625032S"
}

Stančić, A., Drvenica, I., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 72(3), 379-391.
https://doi.org/10.2298/ABS200625032S

Stančić A, Drvenica I, Bugarski B, Ilić V, Bugarski D. Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation. in Archives of Biological Sciences. 2020;72(3):379-391.
doi:10.2298/ABS200625032S .

Stančić, Ana, Drvenica, Ivana, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Extracellular xenogeneic hemoglobin suppresses the capacity for C2C12 myoblast myogenic differentiation" in Archives of Biological Sciences, 72, no. 3 (2020):379-391,
https://doi.org/10.2298/ABS200625032S . .

TY  - JOUR
AU  - Stančić, Ana
AU  - Drvenica, Ivana
AU  - Obradović, Hristina
AU  - Bugarski, Branko
AU  - Ilić, Vesna
AU  - Bugarski, Diana
PY  - 2020
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/1038
AB  - We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro
EP  - 920
SP  - 909
VL  - 144
DO  - 10.1016/j.ijbiomac.2019.09.167
ER  - 

@article{
author = "Stančić, Ana and Drvenica, Ivana and Obradović, Hristina and Bugarski, Branko and Ilić, Vesna and Bugarski, Diana",
year = "2020",
abstract = "We have tested in vitro effects of hemoglobin from bovine slaughterhouse blood (BHb) on stromal cells of mesodermal origin, with an aim to explore its use as a component of cell culture media. Human peripheral blood mesenchymal stromal cells (PB-MSCs) and three mouse cell lines (ATDC5, MC3T3-E1 and 3T3-L1) were employed to study BHb effects on their growth and migration. The cells multilineage differentiation capacity in the presence of BHb was evaluated after induced differentiation, by histochemical staining and by RT-PCR analysis of the expression of genes specific for chondrogenic, adipogenic and osteogenic lineages. The effects of BHb on the cell proliferation and motility were dependent on both, cell type and BHb concentration (0.1 mu M,1 mu M and 10 mu M). In the lowest concentration (0.1 mu M) BHb showed the least prominent effect on the cell proliferation and migration. In this concentration BHb reduced the differentiation capacity of all tested cells and its effect was dependent of composition of induction medium and the culture period. Obtained data suggest that BHb has the potential to be used as a component of cell culture media through maintaining proliferation and reducing differentiation capacity of mesenchymal stromal cells.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro",
pages = "920-909",
volume = "144",
doi = "10.1016/j.ijbiomac.2019.09.167"
}

Stančić, A., Drvenica, I., Obradović, H., Bugarski, B., Ilić, V.,& Bugarski, D.. (2020). Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 144, 909-920.
https://doi.org/10.1016/j.ijbiomac.2019.09.167

Stančić A, Drvenica I, Obradović H, Bugarski B, Ilić V, Bugarski D. Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro. in International Journal of Biological Macromolecules. 2020;144:909-920.
doi:10.1016/j.ijbiomac.2019.09.167 .

Stančić, Ana, Drvenica, Ivana, Obradović, Hristina, Bugarski, Branko, Ilić, Vesna, Bugarski, Diana, "Native bovine hemoglobin reduces differentiation capacity of mesenchymal stromal cells in vitro" in International Journal of Biological Macromolecules, 144 (2020):909-920,
https://doi.org/10.1016/j.ijbiomac.2019.09.167 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić Đorđević, Ivana
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/950
AB  - Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.
PB  - Wiley, Hoboken
T2  - Cell Proliferation
T1  - IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells
IS  - 1
VL  - 52
DO  - 10.1111/cpr.12533
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Mojsilović, Slavko and Okić Đorđević, Ivana and Obradović, Hristina and Krstić, Jelena and Jauković, Aleksandra and Bugarski, Diana",
year = "2019",
abstract = "Objectives Soluble IL-33 (interleukin (IL)-1-like cytokine) acts as endogenous alarm signal (alarmin). Since alarmins, besides activating immune system, act to restore tissue homeostasis, we investigated whether IL-33 exerts beneficial effects on oral stem cell pull. Materials and Methods Clonogenicity, proliferation, differentiation and senescence of stem cells derived from human periodontal ligament (PDLSCs) and dental pulp (DPSCs) were determined after in vitro exposure to IL-33. Cellular changes were detected by flow cytometry, Western blot, immunocytochemistry and semiquantitative RT-PCR. Results IL-33 stimulated proliferation, clonogenicity and expression of pluripotency markers, OCT-4, SOX-2 and NANOG, but it inhibited ALP activity and mineralization in both PDLSCs and DPSCs. Higher Ki67 expression and reduced beta-galactosidase activity in IL-33-treated cells were demonstrated, whereas these trends were more conspicuous in osteogenic medium. However, after 7-day IL-33 pretreatment, differentiation capacity of IL-33-pretreated cells was retained, and increased ALP activity was observed in both cell types. Results showed that IL-33 regulates NF-kappa B and beta-catenin signalling, indicating the association of these molecules with changes observed in IL-33-treated PDLSCs and DPSCs, particularly their proliferation, pluripotency-associated marker expression and osteogenesis. Conclusions IL-33 treatment impairs osteogenesis of PDLSCs and DPSCs, while increases their clonogenicity, proliferation and pluripotency marker expression. After exposure to IL-33, osteogenic capacity of cells stayed intact. NF-kappa B and beta-catenin are implicated in the effects achieved by IL-33 in PDLSCs and DPSCs.",
publisher = "Wiley, Hoboken",
journal = "Cell Proliferation",
title = "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells",
number = "1",
volume = "52",
doi = "10.1111/cpr.12533"
}

Kukolj, T., Trivanović, D., Mojsilović, S., Okić Đorđević, I., Obradović, H., Krstić, J., Jauković, A.,& Bugarski, D.. (2019). IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation
Wiley, Hoboken., 52(1).
https://doi.org/10.1111/cpr.12533

Kukolj T, Trivanović D, Mojsilović S, Okić Đorđević I, Obradović H, Krstić J, Jauković A, Bugarski D. IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells. in Cell Proliferation. 2019;52(1).
doi:10.1111/cpr.12533 .

Kukolj, Tamara, Trivanović, Drenka, Mojsilović, Slavko, Okić Đorđević, Ivana, Obradović, Hristina, Krstić, Jelena, Jauković, Aleksandra, Bugarski, Diana, "IL-33 guides osteogenesis and increases proliferation and pluripotency marker expression in dental stem cells" in Cell Proliferation, 52, no. 1 (2019),
https://doi.org/10.1111/cpr.12533 . .

TY  - JOUR
AU  - Obradović, Hristina
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Mojsilović, Slavko
AU  - Okić, Ivana
AU  - Kukolj, Tamara
AU  - Ilić, Vesna
AU  - Jauković, Aleksandra
AU  - Terzić, Milan
AU  - Bugarski, Diana
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/969
AB  - Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.
PB  - W B Saunders Co Ltd, London
T2  - Placenta
T1  - Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche
EP  - 34
SP  - 25
VL  - 82
DO  - 10.1016/j.placenta.2019.05.005
ER  - 

@article{
author = "Obradović, Hristina and Krstić, Jelena and Trivanović, Drenka and Mojsilović, Slavko and Okić, Ivana and Kukolj, Tamara and Ilić, Vesna and Jauković, Aleksandra and Terzić, Milan and Bugarski, Diana",
year = "2019",
abstract = "Introduction: Mesenchymal stem cells from Wharton's Jelly of a human umbilical cord (WJ-MSCs) are a potential tool in regenerative medicine based on their availability, proliferative potential and differentiation capacity. Since their physiological niche contains low oxygen levels, we investigated whether cultivation of WJ-MSCs at 3% O-2 affects their main features. Methods: WJ-MSCs were cultured under 21% and 3% O-2. Proliferation rate was followed by short and long term proliferation assays, clonogenic capacity by CFU-F assay and cell cycle and death by flow cytometry. Differentiation capacity was investigated by histochemical staining after induced differentiation. Pluripotency and differentiation markers' expression was determined by RT-PCR. Migration capacity was followed by scratch assay and mobilization from collagen, and the activity of proteolytic enzymes by zymography. Specific inhibitors of MAPK and Wnt/beta-catenin pathways were used to investigate underlying molecular mechanisms. Results: Compared to standard 21% O-2, cultivation of WJ-MSCs at 3% O-2 did not influence their immunophenotype, while it modulated their differentiation process and enhanced their clonogenic and expansion capacity. 3% O-2 induced transient change in cell cycle and prevented cell death. The expression of NANOG, OCT4A, OCT4B and SOX2 was increased at 3% O-2. Both cultivation and preculturing of WJ-MSCs at 3% O-2 increased their in vitro migratory capacity and enhanced the activity of proteolytic enzymes. ERK1/2 mediated WJ-MSCs' mobilization from collagen regardless of oxygen levels, while Wnt/beta-catenin pathway was activated during migration and mobilization at standard conditions. Conclusion: Culturing of WJ-MSCs under 3% O-2 should be considered a credible condition when investigating their properties and potential use.",
publisher = "W B Saunders Co Ltd, London",
journal = "Placenta",
title = "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche",
pages = "34-25",
volume = "82",
doi = "10.1016/j.placenta.2019.05.005"
}

Obradović, H., Krstić, J., Trivanović, D., Mojsilović, S., Okić, I., Kukolj, T., Ilić, V., Jauković, A., Terzić, M.,& Bugarski, D.. (2019). Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta
W B Saunders Co Ltd, London., 82, 25-34.
https://doi.org/10.1016/j.placenta.2019.05.005

Obradović H, Krstić J, Trivanović D, Mojsilović S, Okić I, Kukolj T, Ilić V, Jauković A, Terzić M, Bugarski D. Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche. in Placenta. 2019;82:25-34.
doi:10.1016/j.placenta.2019.05.005 .

Obradović, Hristina, Krstić, Jelena, Trivanović, Drenka, Mojsilović, Slavko, Okić, Ivana, Kukolj, Tamara, Ilić, Vesna, Jauković, Aleksandra, Terzić, Milan, Bugarski, Diana, "Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche" in Placenta, 82 (2019):25-34,
https://doi.org/10.1016/j.placenta.2019.05.005 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/960
AB  - We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy
EP  - 390
SP  - 384
VL  - 213
DO  - 10.1016/j.saa.2019.01.069
ER  - 

@article{
author = "Lazarević, J. J. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "We have employed micro-Raman spectroscopy to get insight into intrinsic biomolecular profile of individual mesenchymal stem cell isolated from periodontal ligament. Furthermore, these cells were stimulated towards adipogenic, chondrogenic, and osteogenic lineages and their status of differentiation was assessed using micro-Raman spectroscopy. In both cases, glass coverslips were used as substrates, due to their wide availability and cost effectiveness. In all sample groups, the same type of behavior was observed, manifested as changes in Raman spectra: the increase of relative intensity of protein/lipid bands and decrease of nucleic acid bands. Comprehensive statistical analysis in the form of principal component analysis was performed, which revealed noticeable grouping of cells with the similar features. Despite the inhomogeneity of primary stem cells and their differentiated lineages, we demonstrated that micro-Raman spectroscopy is sufficient for distinguishing cells' status, which can be valuable for medical and clinical application.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy",
pages = "390-384",
volume = "213",
doi = "10.1016/j.saa.2019.01.069"
}

Lazarević, J. J., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 213, 384-390.
https://doi.org/10.1016/j.saa.2019.01.069

Lazarević JJ, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;213:384-390.
doi:10.1016/j.saa.2019.01.069 .

Lazarević, J. J., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Probing primary mesenchymal stem cells differentiation status by micro-Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 213 (2019):384-390,
https://doi.org/10.1016/j.saa.2019.01.069 . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Ralević, U.
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Lazarević, N.
AU  - Bugarski, Branko
AU  - Popović, Z., V
PY  - 2019
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/966
AB  - In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
T1  - Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy
EP  - 178
SP  - 173
VL  - 216
DO  - 10.1016/j.saa.2019.03.012
ER  - 

@article{
author = "Lazarević, J. J. and Ralević, U. and Kukolj, Tamara and Bugarski, Diana and Lazarević, N. and Bugarski, Branko and Popović, Z., V",
year = "2019",
abstract = "In investigation of (patho)physiological processes, cells represent frequently used analyte as an exceptional source of information. However, spectroscopic analysis of live cells is still very seldom in clinics, as well as in research studies. Among others, the reasons are long acquisition time during which autolysis process is activated, necessity of specified technical equipment, and inability to perform analysis in a moment of sample preparation. Hence, an optimal method of preserving cells in the existing state is of extreme importance, having in mind that selection of fixative is cell lineage dependent. In this study, two commonly used chemical fixatives, formaldehyde and methanol, are used for preserving primary mesenchymal stem cells extracted from periodontal ligament, which are valuable cell source for reconstructive dentistry. By means of Raman spectroscopy, cell samples were probed and the impact of these fixatives on their Raman response was analyzed and compared. Different chemical mechanisms are the core processes of formaldehyde and methanol fixation and certain Raman bands are shifted and/or of changed intensity when Raman spectra of cells fixed in that manner are compared. In order to get clearer picture, comprehensive statistical analysis was performed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy",
title = "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy",
pages = "178-173",
volume = "216",
doi = "10.1016/j.saa.2019.03.012"
}

Lazarević, J. J., Ralević, U., Kukolj, T., Bugarski, D., Lazarević, N., Bugarski, B.,& Popović, Z., V.. (2019). Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 216, 173-178.
https://doi.org/10.1016/j.saa.2019.03.012

Lazarević JJ, Ralević U, Kukolj T, Bugarski D, Lazarević N, Bugarski B, Popović ZV. Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy. in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy. 2019;216:173-178.
doi:10.1016/j.saa.2019.03.012 .

Lazarević, J. J., Ralević, U., Kukolj, Tamara, Bugarski, Diana, Lazarević, N., Bugarski, Branko, Popović, Z., V, "Influence of chemical fixation process on primary mesenchymal stem cells evidenced by Raman spectroscopy" in Spectrochimica Acta Part A-Molecular & Biomolecular Spectroscopy, 216 (2019):173-178,
https://doi.org/10.1016/j.saa.2019.03.012 . .

TY  - JOUR
AU  - Trivanović, Drenka
AU  - Drvenica, Ivana
AU  - Kukolj, Tamara
AU  - Obradović, Hristina
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Bugarski, Branko
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/835
AB  - Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Artificial Cells Nanomedicine & Biotechnology
T1  - Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells
EP  - S382
SP  - S370
VL  - 46
DO  - 10.1080/21691401.2018.1494183
ER  - 

@article{
author = "Trivanović, Drenka and Drvenica, Ivana and Kukolj, Tamara and Obradović, Hristina and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Bugarski, Branko and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Adipose tissue (AT) homeostasis and expansion are dependent on complex crosstalk between resident adipose stromal/stem cells (ASCs) and AT extracellular matrix (ECM). Although adipose tissue ECM (atECM) is one of the key players in the stem cell niche, data on bidirectional interaction of ASCs and atECM are still scarce. Here, we investigated how atECM guides ASCs' differentiation. atECM altered shape and cytoskeleton organization of ASCs without changing their proliferation, beta-galactosidase activity and adhesion. Cytoskeleton modifications occurred due to fostered parallel organization of F-actin and elevated expression of Vimentin in ASCs. After seven-day cultivation, atECM impaired osteogenesis of ASCs, simultaneously decreasing expression of Runx2. In addition, atECM accelerated early adipogenesis concomitantly with altered Vimentin organization in ASCs, slightly increasing PPAR, while elevated Adiponectin and Vimentin mRNA expression. Early adipogenesis triggered by atECM was followed by upregulated mitochondrial activity and Sirtuin 1 (SIRT1) expression in ASCs. Proadipogenic events induced by atECM were mediated by SIRT1, indicating the supportive role of atECM in adipogenesis-related metabolic state of ASCs. These results provide a closer look at the effects of atECM on ASC physiology and may support the advancement of engineering design in soft tissue reconstruction and fundamental research of AT.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Artificial Cells Nanomedicine & Biotechnology",
title = "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells",
pages = "S382-S370",
volume = "46",
doi = "10.1080/21691401.2018.1494183"
}

Trivanović, D., Drvenica, I., Kukolj, T., Obradović, H., Okić Đorđević, I., Mojsilović, S., Krstić, J., Bugarski, B., Jauković, A.,& Bugarski, D.. (2018). Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology
Taylor & Francis Ltd, Abingdon., 46, S370-S382.
https://doi.org/10.1080/21691401.2018.1494183

Trivanović D, Drvenica I, Kukolj T, Obradović H, Okić Đorđević I, Mojsilović S, Krstić J, Bugarski B, Jauković A, Bugarski D. Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells. in Artificial Cells Nanomedicine & Biotechnology. 2018;46:S370-S382.
doi:10.1080/21691401.2018.1494183 .

Trivanović, Drenka, Drvenica, Ivana, Kukolj, Tamara, Obradović, Hristina, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Bugarski, Branko, Jauković, Aleksandra, Bugarski, Diana, "Adipoinductive effect of extracellular matrix involves cytoskeleton changes and SIRT1 activity in adipose tissue stem/stromal cells" in Artificial Cells Nanomedicine & Biotechnology, 46 (2018):S370-S382,
https://doi.org/10.1080/21691401.2018.1494183 . .

TY  - JOUR
AU  - Kukolj, Tamara
AU  - Trivanović, Drenka
AU  - Okić Đorđević, Ivana
AU  - Mojsilović, Slavko
AU  - Krstić, Jelena
AU  - Obradović, Hristina
AU  - Janković, Srdja
AU  - Santibanez, Juan F.
AU  - Jauković, Aleksandra
AU  - Bugarski, Diana
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/873
AB  - Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (Escherichia coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability, and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP, and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPAR mRNA expression. LPS promoted myofibroblast-like phenotype of PDLSCs, since it significantly enhanced PDLSC contractility, as well as protein and/or gene expression of TGF-beta, fibronectin (FN), alpha-SMA, and NG2. LPS also increased protein and gene expression levels of anti-inflammatory COX-2 and pro-inflammatory IL-6 molecules in PDLSCs. Inhibition of peripheral blood mononuclear cells (MNCs) transendothelial migration in presence of LPS-treated PDLSCs was accompanied by the reduction of CD29 expression within MNCs. However, LPS treatment did not change the inhibitory effect of PDLSCs on mitogen-stimulated proliferation of CD4(+) and the ratio of CD4(+)CD25(high)/CD4(+)CD25(low) lymphocytes. LPS-treated PDLSCs did not change the frequency of CD34(+) and CD45(+) cells, but decreased the frequency of CD33(+) and CD14(+) myeloid cells within MNCs. Moreover, LPS treatment attenuated the stimulatory effect of PDLSCs on CFC activity of MNCs, predominantly the CFU-GM number. The results indicated that LPS-activated ERK1,2 was at least partly involved in the observed effects on PDLSC differentiation capacity, acquisition of myofibroblastic attributes, and changes of their immunomodulatory features.
PB  - Wiley, Hoboken
T2  - Journal of Cellular Physiology
T1  - Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling
EP  - 462
IS  - 1
SP  - 447
VL  - 233
DO  - 10.1002/jcp.25904
ER  - 

@article{
author = "Kukolj, Tamara and Trivanović, Drenka and Okić Đorđević, Ivana and Mojsilović, Slavko and Krstić, Jelena and Obradović, Hristina and Janković, Srdja and Santibanez, Juan F. and Jauković, Aleksandra and Bugarski, Diana",
year = "2018",
abstract = "Lipopolysaccharide (LPS) is a pertinent deleterious factor in oral microenvironment for cells which are carriers of regenerative processes. The aim of this study was to investigate the emerging in vitro effects of LPS (Escherichia coli) on human periodontal ligament stem cell (PDLSC) functions and associated signaling pathways. We demonstrated that LPS did not affect immunophenotype, proliferation, viability, and cell cycle of PDLSCs. However, LPS modified lineage commitment of PDLSCs inhibiting osteogenesis by downregulating Runx2, ALP, and Ocn mRNA expression, while stimulating chondrogenesis and adipogenesis by upregulating Sox9 and PPAR mRNA expression. LPS promoted myofibroblast-like phenotype of PDLSCs, since it significantly enhanced PDLSC contractility, as well as protein and/or gene expression of TGF-beta, fibronectin (FN), alpha-SMA, and NG2. LPS also increased protein and gene expression levels of anti-inflammatory COX-2 and pro-inflammatory IL-6 molecules in PDLSCs. Inhibition of peripheral blood mononuclear cells (MNCs) transendothelial migration in presence of LPS-treated PDLSCs was accompanied by the reduction of CD29 expression within MNCs. However, LPS treatment did not change the inhibitory effect of PDLSCs on mitogen-stimulated proliferation of CD4(+) and the ratio of CD4(+)CD25(high)/CD4(+)CD25(low) lymphocytes. LPS-treated PDLSCs did not change the frequency of CD34(+) and CD45(+) cells, but decreased the frequency of CD33(+) and CD14(+) myeloid cells within MNCs. Moreover, LPS treatment attenuated the stimulatory effect of PDLSCs on CFC activity of MNCs, predominantly the CFU-GM number. The results indicated that LPS-activated ERK1,2 was at least partly involved in the observed effects on PDLSC differentiation capacity, acquisition of myofibroblastic attributes, and changes of their immunomodulatory features.",
publisher = "Wiley, Hoboken",
journal = "Journal of Cellular Physiology",
title = "Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling",
pages = "462-447",
number = "1",
volume = "233",
doi = "10.1002/jcp.25904"
}

Kukolj, T., Trivanović, D., Okić Đorđević, I., Mojsilović, S., Krstić, J., Obradović, H., Janković, S., Santibanez, J. F., Jauković, A.,& Bugarski, D.. (2018). Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. in Journal of Cellular Physiology
Wiley, Hoboken., 233(1), 447-462.
https://doi.org/10.1002/jcp.25904

Kukolj T, Trivanović D, Okić Đorđević I, Mojsilović S, Krstić J, Obradović H, Janković S, Santibanez JF, Jauković A, Bugarski D. Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling. in Journal of Cellular Physiology. 2018;233(1):447-462.
doi:10.1002/jcp.25904 .

Kukolj, Tamara, Trivanović, Drenka, Okić Đorđević, Ivana, Mojsilović, Slavko, Krstić, Jelena, Obradović, Hristina, Janković, Srdja, Santibanez, Juan F., Jauković, Aleksandra, Bugarski, Diana, "Lipopolysaccharide can modify differentiation and immunomodulatory potential of periodontal ligament stem cells via ERK1,2 signaling" in Journal of Cellular Physiology, 233, no. 1 (2018):447-462,
https://doi.org/10.1002/jcp.25904 . .

TY  - JOUR
AU  - Krstić, Jelena
AU  - Trivanović, Drenka
AU  - Obradović, Hristina
AU  - Kukolj, Tamara
AU  - Bugarski, Diana
AU  - Santibanez, Juan F.
PY  - 2018
UR  - http://rimi.imi.bg.ac.rs/handle/123456789/858
AB  - The ability to differentiate into cells of different lineage, such as muscle, bone, cartilage and fat, is the chief value of adult mesenchymal stem cells (MSCs) which can be used with the final aim to regenerate damaged tissue. Due to potential use, as well as importance in tissue development, a number of questions have been raised regarding the molecular mechanisms of MSC differentiation. As one of the crucial mediators in organism development, transforming growth factor beta (TGF-beta) superfamily directs MSCs commitment in the selection of differentiation pathways. In this review we aim to give an overview of the current knowledge on the mechanisms of MSCs differentiation, on the involvement of TGF-beta superfamily in MSCs differentiation with additional insight into the mutual regulation of microRNAs and TGF-beta in MSCs differentiation. Particular focus has been given to the signaling and transcriptional networks governing the differentiation processes.
PB  - Bentham Science Publ Ltd, Sharjah
T2  - Current Protein & Peptide Science
T1  - Regulation of Mesenchymal Stem Cell Differentiation by Transforming Growth Factor Beta Superfamily
EP  - 1154
IS  - 12
SP  - 1138
VL  - 19
DO  - 10.2174/1389203718666171117103418
ER  - 

@article{
author = "Krstić, Jelena and Trivanović, Drenka and Obradović, Hristina and Kukolj, Tamara and Bugarski, Diana and Santibanez, Juan F.",
year = "2018",
abstract = "The ability to differentiate into cells of different lineage, such as muscle, bone, cartilage and fat, is the chief value of adult mesenchymal stem cells (MSCs) which can be used with the final aim to regenerate damaged tissue. Due to potential use, as well as importance in tissue development, a number of questions have been raised regarding the molecular mechanisms of MSC differentiation. As one of the crucial mediators in organism development, transforming growth factor beta (TGF-beta) superfamily directs MSCs commitment in the selection of differentiation pathways. In this review we aim to give an overview of the current knowledge on the mechanisms of MSCs differentiation, on the involvement of TGF-beta superfamily in MSCs differentiation with additional insight into the mutual regulation of microRNAs and TGF-beta in MSCs differentiation. Particular focus has been given to the signaling and transcriptional networks governing the differentiation processes.",
publisher = "Bentham Science Publ Ltd, Sharjah",
journal = "Current Protein & Peptide Science",
title = "Regulation of Mesenchymal Stem Cell Differentiation by Transforming Growth Factor Beta Superfamily",
pages = "1154-1138",
number = "12",
volume = "19",
doi = "10.2174/1389203718666171117103418"
}

Krstić, J., Trivanović, D., Obradović, H., Kukolj, T., Bugarski, D.,& Santibanez, J. F.. (2018). Regulation of Mesenchymal Stem Cell Differentiation by Transforming Growth Factor Beta Superfamily. in Current Protein & Peptide Science
Bentham Science Publ Ltd, Sharjah., 19(12), 1138-1154.
https://doi.org/10.2174/1389203718666171117103418

Krstić J, Trivanović D, Obradović H, Kukolj T, Bugarski D, Santibanez JF. Regulation of Mesenchymal Stem Cell Differentiation by Transforming Growth Factor Beta Superfamily. in Current Protein & Peptide Science. 2018;19(12):1138-1154.
doi:10.2174/1389203718666171117103418 .

Krstić, Jelena, Trivanović, Drenka, Obradović, Hristina, Kukolj, Tamara, Bugarski, Diana, Santibanez, Juan F., "Regulation of Mesenchymal Stem Cell Differentiation by Transforming Growth Factor Beta Superfamily" in Current Protein & Peptide Science, 19, no. 12 (2018):1138-1154,
https://doi.org/10.2174/1389203718666171117103418 . .